The latest-generation geostationary meteorological satellite, Fengyun-4B (FY-4B), equipped with the Geostationary Interferometric Infrared Sounder (GIIRS), offers high-spatiotemporal-resolution three-dimensional temperature structures. Its deployment serves as a critical complement to atmospheric temperature profile (ATP) observation in the Tibetan Plateau (TP). Based on radiosonde observation (RAOB) and the fifth-generation ECMWF global climate atmospheric reanalysis (ERA5), this study validates the availability and representativeness of FY-4B/GIIRS ATP products in the eastern TP region. Due to the issue of satellite zenith, this study focuses solely on examining the eastern TP region. Under a clear sky, FY-4B/GIIRS ATP exhibits good consistency with RAOB compared to cloudy conditions, with an average root mean square error (RMSE) of 2.57 K. FY-4B/GIIRS tends to underestimate temperatures in the lower layers while overestimating temperatures in the upper layers. The bias varies across seasons. Except for summer, the horizontal and vertical bias distribution patterns are similar, though there are slight differences in values. Despite the presence of bias, FY-4B/GIIRS ATP maintains a good consistency with observations and reanalysis data, indicating commendable product quality. These results demonstrate that it can play a vital role in augmenting the ATP observation network limited by sparse radiosonde stations in the eastern TP, offering crucial data support for numerical weather prediction, weather monitoring, and related meteorological research in this region.