Areca Nut Research Articles

Potensi Sektor Pertanian Dalam Pembangunan Wilayah Kabupaten Pasaman

Regional development aims to increase regional economic growth, reduce regional disparities and increase regional competitiveness. The agricultural sector is a sector that has an important role in the regional economy. In an effort to accelerate regional development growth, further analysis of the development of the agricultural sector is needed. The objectives of this research are 1) to analyze the development of the agricultural sector in Pasaman Regency, 2) to analyze the pattern and structure of the agricultural sector in Pasaman Regency. The analysis method used is the Entropy Diversity Index (IDE) and Klassen Typology. The results of the analysis are that the agricultural sector in Pasaman Regency is not developing because it has an IDE value < 1, rice, corn, banana and rambutan are included in the leading commodities (quadrant I), durian and palm oil are included in the potential sector (quadrant II), salak, rubber , cocoa, areca nut and coconut are included as commodities. developing (quadrant III), cassava, sweet potatoes, peanuts and oranges are among the underdeveloped sectors (quadrant IV).

Amplification of intense red emission through integrating Li+ ions to V2O5:Eu3+ phosphors: Optical thermometry, cheiloscopy and extraction of level III features for individual identification

This study presents the synthesis of V2O5:Eu3+ phosphors in concentrations ranging from 1 to 9 mol %, using a solution combustion method with Areca Nut (A.N) as a bio-fuel. The samples exhibit an orthorhombic crystal structure, confirmed by powder X-ray diffraction (PXRD), and rod-like morphology as shown by field emission scanning electron microscopy (FE-SEM). Photoluminescence (PL) studies reveal that the emission intensity of the 5D0→7F2 transition at 619 nm increases with the Eu3+ concentration up to 5 mol %, where it peaks before quenching due to quadruple-quadruple interactions, according to the Van Uitert relation. To improve luminescence, Li+ ions are added, with the V2O5:5Eu3+ co-doped with 4Li+ (V2O5:5Eu3+, 4Li+) ions showing the highest PL intensity, about 5.03 times higher than that of Eu3+ alone. This optimized phosphor has Commission Internationale de l’Eclairage (CIE) chromaticity coordinates of (0.6022, 0.3963), closely matching commercial red-emitting phosphors and demonstrating a colour purity (CP) of 88.69 %. Thermal stability assessments indicate that these phosphors have a high activation energy (Ea) of about 0.224 eV, signifying robust thermal properties. The optimized V2O5:5Eu3+, 4Li+ phosphors efficiently reveal level I–III features in latent fingerprints (LFPs) and accurately identify lip prints (LPs) groove patterns of type I–VI under 365 nm UV light, providing high resolution and contrast. These characteristics make the optimized phosphor an excellent candidate for use in photonic devices, advanced forensic and data security applications.

Differential effects of areca nut, tobacco, and lime on oral squamous cell carcinoma progression

Differential effects of areca nut, tobacco, and lime on oral squamous cell carcinoma progression

Oral Submucous Fibrosis - Case Series

Oralsubmucous fibrosis is regarded as a collagen metabolic disorder with an overall increased collagen production and decreased collagen degradation resulting in increased collagen deposition in the oral tissues, and fibrosis due to alkaloid exposure. We report three case reports of oral submucous fibrosis. Clinical findings and histopathologic findings were consistent with the diagnosis of stage 2 oral submucous fibrosis. The patient was educated on the effects of areca nut and chances of malignant transformation. Keywords Malignant Transformation, Oral Submucous Fibrosis, Pathogenesis, Management

Current advances of oral cancer in Taiwan.

To provide updated statistics of oral cancer incidence and mortality in Taiwan, and to describe recent developments for oral cancer control to reduce oral cancer incidence and mortality. The Taiwan Cancer Registry Annual Report 2023, the Taiwan Cancer Registry Online Interactive Search System, and the 2023 Health Promotion Administration Annual Report are consulted and summarized. A literature search using PubMed was also conducted to identify Taiwan oral cancer control-related studies. Taiwan, with a long history of areca/betel quid chewing, has battled with oral cancer for decades. In 2021, the male lip and oral cavity cancer incidence is 29.77 (per 100,000 persons) and mortality is 11.25. Over these years, the male chewing prevalence was reduced to 6.2% in 2018. Taiwan has a sustained national oral cancer screening programme provided to high-risk groups biennially. Although the age-standardized incidence rates have been slowly decreasing since 2009, due to Taiwan's ageing population, the actual oral cancer burden is still stringent. The collaboration of multi-ministries and agencies on promoting areca nut and betel quid control, improving oral cancer awareness, and delivering cancer care remains essential to prevent oral cancer and reduce oral cancer deaths.

Intelligent treatment system based on bioinformatics and neuro-immune-digestive tract diseases

There are currently many clinical issues plaguing digestive oncologists, including the impact of dietary habits on the occurrence of early gastrointestinal cancer, the association between irritable bowel syndrome, ulcerative colitis, and early gastrointestinal cancer, the expected changes in gastrointestinal cancer after IBS, IBD, and susceptibility gene mutations, and the mechanisms by which susceptibility gene mutations in gastrointestinal cancer lead to different diseases. Meanwhile, as a carcinogen, betel nut is also prone to cancer and emotional disorders, and there are also many doctor-patient conflicts in the current clinical treatment of digestive tract tumors. To address the aforementioned issues, the research team analyzed dietary habits data using SOM clustering algorithm and isolated forest algorithm. Based on bioinformatics, new therapies have been developed for the pathogenesis and immune pathways of various cancers in the digestive system. Using network pharmacology methods, explore the toxicological mechanism of betel nut and its mechanism in mediating emotional disorders. Molecular docking is used to elucidate drug prediction. Based on four AI algorithms, the occurrence of gastrointestinal cancer was predicted through dietary habits. Using computer vision technology to address the deficiencies in early clinical reporting of cancer. This article provides clinical doctors with new treatment tools.

Transforming Hospital Housekeeping: The Kayakalp Journey.

The Kayakalp guidelines for public healthcare facilities under the Swachh Bharat Abhiyan (Clean India Mission) focus on improving sanitation, cleanliness, and infection control at public hospitals in India. This study was conducted in a 960-bed tertiary-level teaching hospital in eastern India. Housekeeping has been a challenge in public institutions, with factors like overcrowding and resource constraints. Tobacco and betel nut chewing, spitting, poor sanitation practices, and open urination are major challenges in ensuring sanitation at the hospital. The research objective was to study the implementation of the Kayakalp guidelines for quality improvement in housekeeping services at the institution. A pre- and post-interventional study was conducted using the Plan-Do-Check-Act (PDCA) quality tool. Plan phases included the gap assessment using the Kayakalp checklist with numerical scoring. Necessary interventions were done under three headings: structure, processes, and outcomes in the "Do" phase. The "Check" phase included monitoring of the activities followed by the "Act" phase, which included a review of the action plan. External experts nominated by the government conducted the final assessments and recommended it as one of the cleanest hospitals. A 360-degree improvement was observed in hospital services, with assessment score improvement from 73.68% to 95.0%. The institution received the first prize in 2020 and 2021 and runners-up Kayakalp National Award under category "B" (Institute of National Importance) Hospitals in 2019. The implementation of Kayakalp guidelines of the Government of India proved to be efficient in the improvement of housekeeping and infection control practices in the institution.

Effect of hybridisation on the mechanical and weathering characteristics on jute-arecanut leaf-epoxy composites

ABSTRACT Natural fibres play a crucial role in composites by offering renewable, sustainable, low cost and lightweight alternatives to synthetic fibres, promoting eco-friendly composites. Combination of natural fibres such as jute and areca nut in fabricating hybrid composites with superior properties forms the basis of this study. It was observed that mechanical properties increased with increasing natural fibre content. However, jute composites exhibited better properties (strength and rigidity) than arecanut leaf fibre composites due to their inherent superior properties. Hybridisation of fibres yielded higher properties showing that the combination of jute and areca nut fibres (30 wt % fibre content) is more effective in reinforcing the composites. Weathering characteristics revealed that the water absorption and swelling occurred in composites upon their exposure to external environment, water and chemicals. Moreover, their impact properties also decreased subsequent to weathering. Finite element analysis was carried out on these composites under tensile and flexural loading conditions yielded similar trends with experimental results. Remarkable mechanical properties exhibited due to hybridisation of these composites give them potential to be used in different domains such as automobile interiors, furniture, packaging, etc., with lower cost due to the use of natural fibres.

AI BASED ARECANUT PLANT DISEASE CLASSIFICATION SYSTEM

Arecanut, commonly known as betel nut, is a vital cash crop in many tropical regions, contributing significantly to the agricultural economy. However, like other crops, arecanut plants are susceptible to various diseases that can severely impact yield and quality. Early detection and accurate classification of these diseases are crucial for timely intervention and effective disease management. In this study, we propose an AI-based arecanut plant disease classification system that leverages deep learning techniques to automatically identify and classify different diseases affecting arecanut plants. Convolutional neural networks (CNNs) are employed for feature extraction and disease classification, with transfer learning techniques used to fine-tune pre-trained models on the specific task of arecanut disease recognition. Keywords: Arecanut, betel nut, plant disease classification, deep learning, transfer learning, agricultural AI, disease management. convolutional neural network (CNN).

Areca nut-induced AREG promote oral epithelial cell proliferation, migration, and EMT.

To analyze the biological effect and mechanism of areca nut extract (ANE) on human oral keratinocyte (HOK) cells. The effect of gradient concentration of ANE on the proliferation activity of HOK cells was analyzed by cell counting kit-8 (CCK-8) assays. The differentially expressed genes between the ANE group and control group HOK cells were analyzed by second-generation transcriptome sequencing. Real-time PCR and western blot were, respectively, used to analyze the expression of AREG gene and protein in HOK cells. After AREG gene overexpression or knockdown, the proliferation, migration, and expression of proteins related to epithelial-mesenchymal transformation (EMT), MAPK signal pathway in HOK cells were, respectively, detected by CCK-8, wound healing, transwell, and western blot assays. ANE (500 μg/mL) promoted the proliferation and migration of HOK cells, ANE (2 mg/mL) promoted the EMT of HOK cells, and ANE (50 mg/mL) inhibited the proliferation of HOK cells. AREG knockdown inhibited ANE-induced proliferation and migration of HOK cells, while AREG overexpression promoted the proliferation and migration of HOK cells. Western blot assay showed that ANE activated MAPK signal pathway by upregulating AREG protein in HOK cells. ANE promoted HOK cell proliferation, migration, and EMT by mediating AREG-MAPK signaling pathway.

MiRNA genetic variations associated with the predisposition of oral squamous cell carcinoma in central Indian population

The disease burden of Oral Squamous Cell Carcinoma (OSCC) is rising day-by-day and is expected to rise 62 % through 2035. The chewing of tobacco, areca nut, and betel leaf, poor oral hygiene, and chronic infection are common risk factors of OSCC, but genetic and epigenetic factors also contribute equally. MicroRNAs (miRNAs) are comprised of small, non-coding endogenous RNA that regulate a plethora of biological activities by targeting messenger RNA through degradation or inhibition. Single Nucleotide Polymorphisms (SNPs) in miRNA genes can regulate the development and progression of OSCC. The present study aimed to determine the association between SNPs in miRNA genes (miRSNPs) with the risk of OSCC. A case-control study involving 225 histo-pathologically confirmed OSCC cases and 225 healthy controls was conducted, where 25 miRSNPs were analyzed by iPLEX MassArray analysis. A SNP rs12220909 in MIR4293 showed a highly protective effect (CC vs GG, OR = 0.0431, 95%CI = 0.005–0.323, p = 3e-6). Whereas three SNPs, namely, rs4705342 in MIR143 (CC vs TT, OR = 2.25, 95%CI = 2.00–2.53, p = 0.0008), rs531564 in MIR124 (CC vs GG, OR = 24.18, 95%CI = 3.22–181.37, p = 3e-6), and rs3746444 in MIR499 (AA vs GG, OR = 2.01, 95%CI = 1.32–3.05, p = 0.001) were significantly associated with a higher risk of OSCC. Additionally, NanoString-based nCounter miRNA expression profiling revealed that miR-499a (Log2FC = −1.07), and miR-143 (Log2FC = −1.56) were aberrantly expressed in OSCC tissue. Taken together, the above miSNPs may contribute to the high incidence of OSCC in central India. However, further studies with large cohorts and ethnic stratification are required to validate our findings.

Green iron and syngas production via continuous solar-driven agricultural waste biomass gasification combined with iron(III) oxide reduction

The production of renewable synthetic fuels and chemicals from solar energy and agricultural waste biomass is considered. Solar thermochemical conversion processes offer a promising pathway to a sustainable fuel economy and green chemical industry. This study investigates the continuous solar-driven gasification of agricultural biomass (betel nut waste) combined with iron oxide (Fe2O3) reduction to produce carbon-neutral syngas and green metallic iron in a single process. A thermodynamic analysis of the system was initially conducted to predict the distribution of equilibrium products. Then, on-sun continuous processing was experimentally carried out under different operating conditions, including betel/Fe2O3 molar ratios (0.56–1.5) and temperatures (900–1200 °C) to evaluate the process feasibility and reliability. As a result, solar gasification of betel nut waste combined with Fe2O3 reduction performed exceptionally well with continuous reactant particles feeding, demonstrating a feasible pathway for producing green iron and high-quality syngas. The maximum syngas yield reached 63.3 mmol/gdry_betel, approaching its theoretical value, and high-purity Fe was simultaneously produced. The process demonstrated high efficiency, with maximum carbon conversion approaching 98 %, energy upgrade factor up to 1.26, and solar-to-fuel energy conversion efficiency up to 14.4 %, highlighting remarkable conversion performance of biomass and solar energy to chemicals.

Investigation of the properties and weathering behaviour of arecanut sheath fibre reinforced epoxy composites

ABSTRACT Engineered materials having unique elements such as reinforcing phases and matrix materials, such as composites, provide improved stiffness, strength, lightweight characteristics, and corrosion resistance. The utilisation of natural fibre composites, which are derived from sustainable resources such as areca nut sheath, is on the rise because of their affordability, favourable strength-to-weight ratio, and lightweight characteristics. These composites offer a more environmentally friendly option to traditional synthetic fibre composites while also reducing the negative effects of production processes on the environment. The development of these composites has been greatly aided by areca nut sheath fibre epoxy composites, which have applications in construction materials, biodegradable packaging, and other areas. In this research, areca nut sheath reinforced epoxy composites were tested for their mechanical and thermal characteristics along with their durability (weathering). It was observed that areca nut sheath fibre reinforcement improved both the tensile, flexural and impact properties of the composites due to their effective reinforcement, fibre–matrix interaction and load bearing capability. However, this study demonstrates that composites containing 20% areca nut sheath fibres have the best tensile and flexural strengths. Weathering studies revealed that the durability slightly decreased upon exposure to moisture and vermicompost due to moisture intake by the fibres.

TUSC3, p53 and p21 genetic association with development of oral submucous fibrosis and oral squamous cell carcinoma among addictive tobacco chewers of Pakistan

BackgroundThis study delves into the intricate landscape of oral cancer, a global concern with a high incidence in Asian countries. We focus on oral squamous cell carcinoma (OSCC), primarily driven by the consumption of betel nut and its derivatives. OSCC often arises from premalignant lesions like oral submucous fibrosis (OSF). In Pakistan, OSCC is prevalent among men due to various addictive substances, including smokeless tobacco and chewing materials. Mutations in tumor suppressor genes, such as TP53 and p21, play crucial roles in this malignancy’s development. We also explore the involvement of TUSC3 gene deletion in OSCC and OSF.MethodsIn this study we investigated demographics, TUSC3 gene expression, deletion analysis, and TP53 and p21 genetic alterations in OSCC and OSF patients (blood and tissue of 50 samples in each condition) who had tobacco derivates usage history. The association analysis was carried out mainly through PCR based genotyping.ResultsThe study’s patient cohort (OSCC and OSF) displayed a wide age range from 13 to 65 years (Mean = 32.96 years). Both conditions were more prevalent in males, with a male-female ratio of approximately 2.5:1. Chewing habits analysis revealed high frequencies of gutka use in both OSF and OSCC patients. TUSC3 expression analysis in OSCC cell lines indicated significant downregulation. Genotyping showed no TUSC3 deletion in OSF cases, but a deletion rate of over 22% in OSCC tissue samples. Analysis supported a significant association of TUSC3 deletion with OSCC development but not with OSF. Polymorphism in p53 exon 4 and p21 (rs1801270) were significantly associated with both OSCC and OSF, adding to their pathogenesis. Our findings further revealed a strong correlation between TUSC3 deletion and the excessive use of tobacco and related products, shedding light on the genetic underpinnings of OSCC development.ConclusionsNotably, our study provides a crucial insight into genetic aspects underlying OSCC and OSF in response of addictive consumption of areca nut, betel quid, and tobacco derivatives. A significant association between TUSC3 deletion and OSCC development, along with polymorphisms in TP53 and p21, underscores the importance of further research into the molecular mechanisms driving oral cancer progression for improved diagnosis and treatment outcomes.

Extraction and identification of bioactive compounds from areca nut (Areca catechu L.) and potential for future applications

AbstractAreca (Areca catechu L.) nut is a tropical plantation fruit cultivated mainly in South and Southeast Asia. As a chewing hobby, it has become the most common psychoactive substance in the world, besides tobacco, alcohol, and caffeine. Areca catechu contains abundant nutrients and active components such as alkaloids, polyphenols, polysaccharides, proteins, and vitamins, which have been reported to have anti‐inflammatory, antioxidant, antibacterial, anti‐depressant, anti‐hypertensive, anti‐fatigue, and other biological properties. However, at present, the resource utilization rate of the whole‐plant areca nut is low, which not only causes resource waste but also damages the environment. Establishing effective, safe, and environmentally friendly techniques and methods is necessary for the comprehensive utilization of A. catechu resources. In this review, we summarized the traditional and advanced methods for the extraction and identification of main bioactive substances in A. catechu and compared the advantages and disadvantages of these methods. Furthermore, the possible trends and perspectives for future use of A. catechu are also discussed. Our objective is to extend the application of this bioactive ingredient to improve the added value, provide valuable information for developing new A. catechu products and derivatives, and improve the comprehensive utilization of areca nut resources.

Maternal betel quid use during pregnancy and child growth: a cohort study from rural Bangladesh

ABSTRACT Background Chewing betel quid (BQ) – a preparation commonly containing areca nut and slaked lime wrapped in betel leaf – is entrenched in South Asia. Although BQ consumption during pregnancy has been linked to adverse birth outcomes, its effect on postnatal growth remains largely unexplored. Objective We examined the associations of BQ use during pregnancy with children’s height-for-age and body mass index-for-age z-scores (HAZ and BAZ, respectively) and fat and fat-free mass along with sex-based differences in association in rural Bangladesh. Methods With a prospective cohort design, we assessed BQ use among mothers enrolled in the Preterm and Stillbirth Study, Matlab (n = 3140) with a structured questionnaire around early third trimester. Children born to a subset of 614 women (including 134 daily users) were invited to follow-up between October 2021 and January 2022. HAZ and BAZ were calculated from anthropometric assessment, and fat and fat-free mass were estimated using bioelectric impedance. Overall and sex-specific multiple linear regression models were fitted. Results Growth data were available for 501 children (mean age 4.9 years): 43.3% of them were born to non-users, 35.3% to those using prior to or less-than-daily during the survey, and 21.3% to daily users. No statistically significant associations were observed after adjusting for sex, parity, maternal height and education, and household wealth. Conclusions There was no effect of BQ use during pregnancy on postnatal growth in this study. Longitudinal studies following up those born to heavy users beyond childhood are warranted for capturing long-term implications of prenatal BQ exposure.

Oral cancer in India.

To describe the epidemiological status of oral cancer (OC) in India along with updates on risk factors, advances and gaps in preventive measures, treatment, costs of care and monitoring. Two national estimates of incidence and mortality were cited, one for 2016 by the Global Burden of Disease India Study and one based on projections for 2020 by GLOBOCAN. The National Cancer Registry Project provided local and regional incidence and trends using data from 2012-2016. Scientific literature, reports of the NCRP, the IARC and government press releases were also consulted. Tobacco and areca nut habits are the major risk factors for OC in India. In the 1980s, intervention studies on oral screening, awareness generation and habit cessation in rural areas reduced tobacco use and oral leukoplakia. Lately, adoption of the tobacco control law and mass media communications have resulted in decreased tobacco use. However, the increasing popularity of highly carcinogenic tobacco products containing areca nut, with evasion of their bans, is increasing OC incidence. Despite advances in care, there is growing incidence of OC, persisting poor awareness about the need to quit tobacco/areca nut/alcohol use and go for screening. Major efforts are needed to implement preventive activities.

Evaluation of the Role of Trace Elements in Malignant Transformation of Oral Submucous Fibrosis: A Study in the Population of Krishna District, Andhra Pradesh, India.

Oral submucous fibrosis (OSMF) is a chronic, potentially malignant disorder linked predominantly to areca nut chewing. This study investigates the role of serum trace elements (Cu, Fe, Zn, Se) in the progression and malignant transformation of OSMF. A community-based cross-sectional study involved 80 participants from the Government Dental College, Vijayawada, Krishna District, AP. Participants were divided into four groups: areca nut users without OSMF, areca nut users with OSMF, areca nut users with malignant-transformed OSMF, and healthy controls. Blood samples were analyzed for serum iron, copper, zinc, and selenium levels. Significant differences were found in serum copper(P=0.032) and zinc levels (P=0.006), with elevated levels observed in the malignant-transformed OSMF group compared to controls. No significant differences were observed in serum iron (P=0.542) and selenium levels(P=0.062) across the groups. Elevated serum copper levels can serve as a reliable diagnostic marker for malignant transformation in OSMF. Future research should explore the potential therapeutic benefits of copper chelation in OSMF management.

Addressing Human-Elephant Conflict in Sarpang, Bhutan: Challenges and Practices

Asian elephants face threats from human-elephant conflict (HEC), driven by habitat encroachment and fragmentation. In the foothills of the Himalayas, HEC affects a large number of people, causing significant damage to property and crops. Bhutan, sharing elephant habitats with India, faces similar challenges, particularly in the southern regions in Sarpang. We studied the HEC pattern and mitigation strategies adopted by people through a questionnaire survey. Our primary data from the Sarpang division showed that more than 40 % of households experienced HEC. The elephant largely affected maize and paddy along with cash crops such as areca nut, orange, ginger, and cardamom. The study revealed a strong association between crop-raiding incidents and cultivated areas, with most depredations occurring at night. The majority of respondents indicated that crop depredation has increased over the years, which could be linked to the degradation and fragmentation of forest habitat in the landscape. Mitigation measures, such as electric fencing, are preferred but underutilized due to financial constraints. The study emphasizes the need for transboundary cooperation between India and Bhutan, integrating traditional and advanced mitigation strategies, and community involvement. Effective communication, joint patrolling, and habitat restoration are the keys to managing HEC. Transboundary governance should include political and legal support, regional diplomacy, and innovative land use policies.

A study on knowledge level of farmers regarding cultivation practices of areca nut in Chikmagalur district

A study on knowledge level of farmers regarding cultivation practices of areca nut in Chikmagalur district