You have accessJournal of UrologyInfertility: Basic Research & Pathophysiology1 Apr 2018MP60-02 MOLECULAR CHANGES INDUCED BY MIRABEGRON IN RAT SERTOLI CELL PRIMARY CULTURE Mikito Tanaka, Koji Chiba, Takaki Ishida, Kenta Sumii, Teruo Fukuda, Keisuke Okada, Kei Matsushita, and Masato Fujisawa Mikito TanakaMikito Tanaka More articles by this author , Koji ChibaKoji Chiba More articles by this author , Takaki IshidaTakaki Ishida More articles by this author , Kenta SumiiKenta Sumii More articles by this author , Teruo FukudaTeruo Fukuda More articles by this author , Keisuke OkadaKeisuke Okada More articles by this author , Kei MatsushitaKei Matsushita More articles by this author , and Masato FujisawaMasato Fujisawa More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1894AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Beta-adrenoceptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Mirabegron is a selective beta3-adrenoceptor (ADRB3) agonist that has been approved for the treatment of overactive bladder. Studies in rats showed that mirabegron was associated with atrophy and/or reduced weights of the accessory reproductive organs, and in females, prolonged diestrus and decreased numbers of live fetuses. In this study, we evaluated the potential effects of mirabegron on the molecular expressions in rat Sertoli cell (SC) primary culture. METHODS SCs were purified from testes of 18-day-old Sprague-Dawley rats. Primary cultures were treated with 1μM mirabegron in the absence or presence of 10μM U0126, a selective non-competitive inhibitor of Mitogen-Activated Protein (MAP) kinase kinase. Immunofluorescent (IF) study was performed using the primary SC culture to determine the expression of ADRB3 in rat SCs. Protein samples were extracted from whole cell homogenates. Western blot (WB) analysis was performed to explore phosphorylation of MAP kinases. Total RNA was also extracted from SCs. Quantitative real-time RT-PCR (q-PCR) analysis was conducted to determine the expression levels of tight junction related mRNAs. RESULTS IF study showed that ADRB3 was present in rat SCs. WB analyses showed that mirabegron treatment induced the significant activation of p44/42 MAPK. Q-PCR showed that mirabegron treatment led to significant increase in claudin-11 mRNA levels. After additional treatment with U0126, mirabegron-induced up-regulation of Claudin-11 had reduced. CONCLUSIONS These data suggest that ADRB3 is present in rat SCs. ADRB3 may be associated with spermatogenesis through p44/42 MAPK induced tight junction molecules alteration in rat SCs. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e790 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Mikito Tanaka More articles by this author Koji Chiba More articles by this author Takaki Ishida More articles by this author Kenta Sumii More articles by this author Teruo Fukuda More articles by this author Keisuke Okada More articles by this author Kei Matsushita More articles by this author Masato Fujisawa More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...