Beta Values Research Articles

Portfolio Analysis of Financial Institutions in Nepal

In this paper we empirically investigated risk return dynamics of the financial institutions that were selected from the Nepal Stock Exchange for the period of 2016-2022. The objective of this study is to analyze the portfolio of different FIs based on the return and risk parameters using ex-post returns data. This study employed financial and statistical tools to draw the conclusion. The beta values show that commercial banks’ share prices are more volatile than the prices of development banks and finance companies. The alpha coefficients reveal that share prices of all commercial banks and development banks are found overvalued and couple of finance companies is undervalued. The results of correlation coefficient offer a scope of diversification in the Nepalese stock market.

Prognostic effect of DNA methylation of BTG2 gene in Chinese hepatocellular carcinoma

BackgroundThis study aims to develop a prognostic model for overall survival based on potential methylation sites within B-cell translocation gene 2 (BTG2) in Chinese patients with hepatocellular carcinoma (HCC). MethodsThis is a retrospective study. The beta values of nine CpG sites and RSEM normalized count values of BTG2 gene were extracted from the Cancer Genome Atlas-Liver Hepatocellular Carcinoma (TCGA-LIHC) (TCGA-LIHC) dataset, with the beta value representing the methylation level by indicating the ratio of the intensity of the methylated bead type to the combined locus intensity. Pyrosequencing was performed to determine the range of methylation values surrounding cg01798157 site in BTG2 gene. A weighted linear model was developed to predict the overall survival (OS). ResultsThe beta value of cg01798157 was significantly negatively associated with the mRNA expression of BTG2 in the TCGA-LIHC dataset (Spearman's rho = −0.5306, P = 2.27 × 10−27). The methylation level of cg01798157 was significantly associated with OS in the cohort of 51 Chinese HCC patients (Hazard ratio = 0.597, 95% CI: 0.434–0.820, P = 0.001). Multivariate Cox regression analysis identified methylation level of cg01798157, cirrhosis, and microvascular invasion as independent prognostic factors. The prognostic efficiency of death risk score was superior to that of cirrhosis or microvascular invasion alone. ConclusionsThe methylation level of cg01798157 in BTG2 may be an epigenetic biomarker in Chinese patients with resectable HCC.

Association between poverty, low educational level and smoking with adolescent's working memory: cross lagged analysis from longitudinal data.

Working memory is a crucial element of cognitive function. Previous cross-sectional studies have identified various determinants of working memory in children and adolescents. Nonetheless, no study has yet demonstrated the causal relationship of social determinants with working memory in adolescents. This study explores the causal link between the level of education, smoking, and other factors with adolescent's working memory. This study analyzed secondary data from waves 4 and 5 of the Indonesia Family Life Survey (IFLS), utilizing cross-lagged analysis in Jamovi version 2.4.8. The variables included working memory score, maternal education, household income, stress, educational level, smoking, urbanicity, and physical activity. These variables were extracted from IFLS waves 4 and 5, and each dependent variable in IFLS wave four was controlled by the same dependent variables in IFLS 5. Then, we used cross-lagged analysis to assess the causality between each dependent variable and a working memory score in IFLS wave 5. The findings indicate that level of education had a positive impact on working memory in adolescents aged 15-18 years, with a Beta value of 0.18 (95% CI 0.81-0.2; p < 0.001). Smoking and age were negatively associated with working memory, with Beta values of -0.07 (95% CI -0.65 -0.04; p < 0.029) and - 0.10 (95% CI -0.25 -0.05; p < 0.003), respectively. No evidence was found for a significant correlation between poverty and adolescents's working memory. The findings indicate that increased education levels are associated with improved working memory in adolescents aged 15-18. At the same time, smoking has a negative impact on working memory in this age group.

Abstract 1738: Precise detection of benign and malignant renal tumor via epigenetic characteristics of urinary cell-free DNA

Abstract Early detection of clear cell renal cell carcinoma (ccRCC) and accurate differentiation between malignant and benign lesions pose clinical challenges. Profiling the epigenetic pattern of patients through liquid biopsy offers a promising strategy for non-invasive cancer identification. This study utilizes the methylation characteristics of urinary cell-free DNA to enhance the precise diagnosis of ccRCC. In this study, 32 patients with malignant RCC, 20 patients with benign kidney lesions, and 28 age- and sex-matched healthy donors were enrolled. Urine samples were collected and applied by PredicineEPIC assay to analyze the whole genome methylation profiles. We assessed the methylation differences on cancer-specific altered regions and constructed a machine learning model for malignant and benign classification. Beta values were calculated for differentially methylated regions identified through literature mining and our current work. These features carry methylation signals that are recognized by gradient boosting machine and used for classification training. The leave-one-out cross-validation method compensated for the shotage of sample size. The sensitivity and specificity of the optimal model in the training set are both greater than 0.97, and it can achieve an accuracy of 0.95 on the validation set. This performance surpasses traditional diagnostic methods such as contrast-enhanced CT and MRI. This study demonstrated the technical feasibility of leveraging epigenetics profiles to discriminate malignant from benign lesions, suggesting a promising liquid biopsy-based approach for the precise detection of renal cell carcinoma. Citation Format: Wen Kong, Hang Dong, Haoran Tang, Pan Du, Shidong Jia, Jin Zhang. Precise detection of benign and malignant renal tumor via epigenetic characteristics of urinary cell-free DNA [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 1738.

Abstract 886: Integrated deep learning model for predicting DNA methylation and tumor types from histopathology in central nervous system tumors

Abstract Precision in diagnosis of diverse central nervous system (CNS) tumor types is crucial for optimal patient treatment. DNA methylation profiles, which capture the methylation status of thousands of individual CpG sites, are data-driven means to enhance diagnostic accuracy, but this technique is expensive, time-consuming, and not yet routinely available. To address this, we developed DEPLOY, a deep-learning model that predicts 10 major categories of CNS tumors from histopathology. DEPLOY integrates three distinct components: the first classifies CNS tumors directly from histopathology slide images (‘direct model’); the second initially generates predictions for DNA methylation beta values, which are subsequently used for tumor classification (‘indirect model’); and the third classifies tumor types directly from routinely available patient demographics. First, we find that DEPLOY accurately predicted beta values from histopathology images, suggesting that application of inferred methylation data is a promising approach for deep learning histopathology-based classifiers. Using a 10-class model trained on an internal dataset, we apply this model on two independent external test datasets of 1,522 and 348 cases, achieving top-1 accuracies of 96% and 94%, respectively on samples which are predicted with high confidence. Taken together, DEPLOY could assist pathologists in diagnosing CNS tumors, in an equitable very low-cost manner, within a clinically relevant short time frame. Citation Format: Eldad David Shulman, Danh-Tai Hoang, Rust Turakulov, Zied Abdullaev, Omkar Singh, Emma M. Campagnolo, Eric A. Stone, MacLean P. Nasrallah, Eytan Ruppin, Kenneth Aldape. Integrated deep learning model for predicting DNA methylation and tumor types from histopathology in central nervous system tumors [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 886.

Abstract 7011: The DNA methylation landscape across the TCR loci genes in patients with acute myeloid leukemia

Abstract The T-cell receptor (TCR) repertoire is partly shaped by epigenetic modifications, including DNA methylation. Hypomethylating agents like decitabine are used to treat patients with acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). The precise DNA methylation patterns within the TCR loci and their dynamics associated with HMAs treatments remain underexplored. Here, we analyzed methylation patterns across TCR alpha and beta gene loci in T cells from GSE175758 and GSE67170 datasets, comparing patterns between patients with AML and healthy controls. We also investigated the impact of decitabine on the methylation patterns of TCR gene loci in patients with AML and MDS (GSE175758 and GSE80762).The overall levels of methylation between T cells from healthy donors and patients with AML were significantly different within the TRAV (0.55 vs 0.42, P &amp;lt; 0.001) and TRBV regions (0.70 vs 0.59, P &amp;lt; 0.001) with 46 TRAV and 33 TRBV sites differentially methylated positions, respectively. However, methylation patterns in HSCs (GSE63409), T cells from healthy donors and patients with AML and those in AML cells were strongly correlated (R &amp;gt; 0.67, range 0.67-0.99, P &amp;lt; 0.001).Because decitabine preferentially acts on leukemic cells and given the high correlation levels between AML cells and T cells across datasets we analyzed the effects of decitabine on TCR DNA methylation patterns in AML cells. In the leukemic cells decitabine treatment led to significant demethylation across 33 and 30 CpG sites within the TRAV and TRBV genes, respectively. In the pre-treatment AML samples higher methylation beta values were observed in differentially methylated positions (DMPs) compared with non-DMPs (TRAV: 0.847 vs 0.407; P &amp;lt; 0.001; TRBV: 0.771 vs 0.538; P = 0.049). In samples largely enriched of myeloid leukemic blasts, we found that TRAV and TRBV loci methylation patterns differ significantly between AML patients with good, moderate, and poor risk patients (P &amp;lt; 0.001). We found TRBV6-1 position 1 and 2 methylation was lower in patients with good risk, compared with intermediate risk (pos1: 0.07 vs 0.13, P=0.04; pos2: 0.1 vs 0.2, P = 0.002) and poor risk (pos 1: 0.07 vs 0.21, P &amp;lt; 0.001; pos 2: 0.1 vs 0.32, P &amp;lt; 0.001) and was lower in patients with intermediate risk compared with poor risk (pos1: 0.13 vs 0.21, p=0.007; pos 2: 0.21 vs 0.32, P = 0.001). The high correlation of TCR DNA methylation patterns between the T cells and the myeloid cells, suggests the need for similar analyses in the context of AML T cells. The study highlights the presence of a conserved TCR loci methylated signatures which is highly correlated between myeloid leukemic cells and T cells and is associated with clinical outcomes, offering a potential avenue for therapeutic intervention. Hypomethylating agents, such as decitabine, can modulate TCR loci methylation patterns. Citation Format: Mateusz Pospiech, John Beckford, Advaith M. Kumar, Mukund Tamizharasan, Jaqueline Brito, Gangning Liang, Serghei Mangul, Houda Alachkar. The DNA methylation landscape across the TCR loci genes in patients with acute myeloid leukemia [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 7011.

Abstract 6150: MMP14 and MKLN1 as colorectal cancer susceptibility genes and a drug repurposing candidate from genome-wide association study in South Korea

Abstract Background: One of the major topics about colorectal cancer (CRC) is to find susceptible genes. Furthermore, functional interpretation of post-GWAS analysis gains attention and various approaches to find drug-repurposing candidates are used in cancer research. Our study aims to identify susceptible genes for CRC and provide drug-repurposing candidates. Methods: CRC patients in Seoul National University Hospital with prospective follow-up were recruited. From blood-derived DNA, genome-wide SNPs were genotyped using the Korea Biobank Array and 409,063 SNPs were extracted. SNP-based logistic regression model was fit for the event of CRC estimating beta values. Furthermore, post-GWAS analysis was conducted using regulatory function, multiple annotation and gene expression database. Drug-repurposing candidate was identified through the pre-trained deep neural network with susceptible genes. Results: We used 500 participants of CRC cases and 1,500 participants of healthy controls. For GWAS, statistically significant associations were mapped to MKLN1, MMP14, PTPN14 and MKL2 genes. For post-GWAS analysis, strong associations were found for MKLN1 (rs75170436, 7q32.3, Beta=-0.90, P-value=5.90×10-13) and MMP14 (rs3751489, 14q11.2, Beta=-1.91, P-value=2.31×10-12). From the drug-repurposing analysis, PPARδ/β agonist (GW0742; Binding score=3.79(MKLN1), 12.06(MMP14)) was identified for both genes with the highest score. Conclusions: We revealed MKLN1 and MMP14 genes as susceptible genes associated with CRC development. Additionally, we identified PPARδ/β agonist (GW0742) as a drug-repurposing candidate for the treatment of CRC. These findings can promote the understanding of CRC development and provide a novel therapeutic candidate for CRC patients in the future. Citation Format: Dabin Yun, Jung-Ho Yang, Sun-Seong Kweon, Jin-ah Sim, Minjung Kim, Ji Won Park, Seung Yong Jeong, Aesun Shin, Nan Song. MMP14 and MKLN1 as colorectal cancer susceptibility genes and a drug repurposing candidate from genome-wide association study in South Korea [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 6150.

Contribution of community monitoring to knowledge of mammal diversity in voluntarily conservation areas in Southern Mexico

In southern Mexico, Voluntarily Designated Conservation Areas (VCA) represent a biological conservation strategy wherein governance and management are entrusted to the territory. Within the VCAs of the La Chinantla region in the state of Oaxaca, Mexico, community monitoring utilizing camera traps has been conducted with the assistance of government programs. This initiative has yielded a substantial number of records for medium and large mammals. Nevertheless, the available information has not undergone systematic analysis, constraining its utility in strategic planning and the evaluation of biodiversity conservation endeavors. This study seeks to highlight the impact of community monitoring in 18 VCAs on understanding the altitudinal distribution of mammal diversity in La Chinantla. The analysis incorporates data from a community monitoring covering 129 camera trap stations (4,384 camera days) strategically positioned along an elevation gradient ranging from 50 to 2000 m above sea level, over the period 2011–2014. We assessed alpha and beta diversity, as well as the community structure of medium and large mammals within three distinct elevation zones. A total of 26 species of medium-sized mammals were documented, revealing distinct mammal assemblages in each zone. However, 15 species were common across all zones. We found that the highest species richness was observed below 400 m, where tropical rainforest vegetation predominates. We also found that the species turnover component had a significant impact on the total beta value. Despite the considerable involvement of local residents in the monitoring program and their acquisition of social, technical, and ecological knowledge, there is still a need to strengthen their capabilities to enhance community monitoring. Finally, fostering collaboration between local communities, academic institutions, and governmental initiatives is essential for the successful conservation of mammals in La Chinanlta.

Abstract P521: Sleep Timing and Duration in Relation to DNA Methylation in Adolescents: An Epigenome-Wide Analysis

Background: Accumulating evidence suggests that both sleep duration and sleep timing can impact metabolic health of adolescents, potentially through epigenetic modification. The objective of this study was to investigate the relationship between sleep duration and timing and DNA methylation, one type of epigenetic modification that may be responsive to changes in sleep. Methods: Cross-sectional epigenome-wide analysis of DNA methylation was conducted to identify sleep-related CpG sites in 269 females and 233 males (14.4±2.1 years on average) participating in the Early Life Exposure in Mexico to ENvironmental Toxicants (ELEMENT) cohort study. Sleep duration and midpoint on weekdays and weekends were assessed using7-day wrist actigraphy (Actigraph GTX-BT), and DNA methylation in blood leukocytes was measured using the Illumina Infinium Methylation EPIC BeadChip. Linear regression was conducted to assess the relationship between sleep variables and DNA methylation at each locus on the array (using beta values), adjusting for sex, age, maternal education, batch effects, monocytes, natural killer cells, B lymphocyte, granulocyte, and naïve CD8 T cells. A false discovery rate (FDR) using the Benjamini-Hochberg method was applied to correct for multiple testing. Sex-stratified associations were also examined. Results: The average sleep duration was 8.5±1.3 hours on weekdays and 9.1±1.3 hours on weekends, and the average sleep midpoint was 3:48AM±1.4 hours on weekdays and 4.48AM±1.3 hours on weekends. At false discovery rate &lt;0.05, there were no significant associations at any of the 762,657 CpG sites. However, at a false discovery rate of &lt;0.2, there were positive associations between weekday sleep duration and 4 CpG sites (i.e., longer sleep duration associated with higher % DNA methylation), including a site in or near CpG islands annotated to the gene DENND2C. Sex-stratified analyses revealed associations were more evident in males. Conclusion: Epigenome-wide analysis identified possible associations between sleep duration and multiple CpG sites, and in a sex-specific manner. Findings suggest that sleep duration may impact the epigenome in a distinct manner from sleep timing.

Pengaruh Praktek Kerja Industri (Prakerin), Informasi Dunia Kerja Dan Motivasi Memasuki Dunia Kerja Terhadap Kesiapan Kerja Siswa SMK

This study aims to analyze the effect of Industrial Work Practice (PRAKERIN), information on the world of work, and motivation to enter the world of work on the work readiness of XII grade students at the Vocational High School for Agricultural Development (SMK PP) Negeri Padang. By using a sample of 105 students, the sampling technique was carried out using Raosoft Sample Size Calculator Software. The results of data analysis show that the variable that has the most influence on the work readiness of vocational students is Industrial Work Practice (PRAKERIN), with a beta value of 0.477. This study highlights the importance of practical experience in shaping vocational students' work readiness, and emphasizes the need for increased information and motivation related to the world of work. The implication of this finding is the need to improve and develop the PRAKERIN program at SMK PP Negeri Padang, as well as the important role of information and motivation in preparing students to enter the world of work. This research contributes to the understanding of the factors that influence vocational students' work readiness, so that it can be the basis for developing more effective educational strategies and policies in the future.

Non-invasive assessment of stimulation-specific changes in cerebral glucose metabolism with functional PET

PurposeFunctional positron emission tomography (fPET) with [18F]FDG allows quantification of stimulation-induced changes in glucose metabolism independent of neurovascular coupling. However, the gold standard for quantification requires invasive arterial blood sampling, limiting its widespread use. Here, we introduce a novel fPET method without the need for an input function.MethodsWe validated the approach using two datasets (DS). For DS1, 52 volunteers (23.2 ± 3.3 years, 24 females) performed Tetris® during a [18F]FDG fPET scan (bolus + constant infusion). For DS2, 18 participants (24.2 ± 4.3 years, 8 females) performed an eyes-open/finger tapping task (constant infusion). Task-specific changes in metabolism were assessed with the general linear model (GLM) and cerebral metabolic rate of glucose (CMRGlu) was quantified with the Patlak plot as reference. We then estimated simplified outcome parameters, including GLM beta values and percent signal change (%SC), and compared them, region and whole-brain-wise.ResultsWe observed higher agreement with the reference for DS1 than DS2. Both DS resulted in strong correlations between regional task-specific beta estimates and CMRGlu (r = 0.763…0.912). %SC of beta values exhibited strong agreement with %SC of CMRGlu (r = 0.909…0.999). Average activation maps showed a high spatial similarity between CMRGlu and beta estimates (Dice = 0.870…0.979) as well as %SC (Dice = 0.932…0.997), respectively.ConclusionThe non-invasive method reliably estimates task-specific changes in glucose metabolism without blood sampling. This streamlines fPET, albeit with the trade-off of being unable to quantify baseline metabolism. The simplification enhances its applicability in research and clinical settings.

Analysis of drug-induced interstitial lung disease caused by herbal medicine using the Japanese Adverse Drug Event Report database

BackgroundDrug-induced interstitial lung disease (DIILD) is a severe adverse event leading to morbidity and mortality. This study evaluated the adverse event indicators of DIILD and time-to-onset profiles following the daily intake of herbal drugs (Scutellariae radix [“ogon” in Japanese], Bupleuri radix [“saiko” in Japanese], and Pinelliae tuber [“hange” in Japanese]) using the Japanese Adverse Drug Event Report database. DIILD was defined in accordance with the Medical Dictionary for Regulatory Activities.MethodsThe Japanese Adverse Drug Event Report database contained 830,079 reports published between April 2004 and April 2023. The association between herbal medicines and DILLD was evaluated using the pharmacovigilance index as the reporting odds ratio (ROR), logistic regression models, propensity score-matching techniques, and Weibull shape parameters.ResultsThe adjusted RORs using multivariate logistic regression models for Scutellariae radix (daily intake), Pinelliae tuber (daily intake), sex (male), age (≥ 60 years), Scutellariae radix (daily intake)*age (≥ 60 years), and Scutellariae radix (daily intake)* Pinelliae tuber (daily intake) were 1.47 (1.36 − 1.59), 1.05 (1.01 − 1.10), 1.45 (1.34 − 1.57), 1.92 (1.74 − 2.11), 3.35 (3.12 − 3.60), and 1.49 (1.46 − 1.53), respectively. DIILD onset profiles were evaluated using the Weibull shape parameter. A logistic plot of daily intake and onset of DIILD was drawn. ROR signals were detected in 32 of 54 herbal medicines, including Scutellariae radix, Bupleuri radix, and Pinelliae tuber. The median duration (days) (interquartile range) to DIILD onset was 36.0 (27.0–63.0) for Saikokaryukotsuboreito, 35.0 (21.0–55.0) for Saireito, and 31.0 (13.5–67.5) for Shosaikoto. The Weibull shape parameter beta (95% confidence interval) values for Saikokaryukotsuboreito, Saireito, and Shosaikoto were 1.36 (1.08–1.67), 1.36 (1.20–1.52), and 1.31 (0.98–1.68), respectively.ConclusionsDIILD demonstrated a dose-dependent to crude drugs. Clinicians should strive for the early detection of DIILD and avoid the inadvertent administration of herbal medicines.

PENGARUH PROMOSI DI MEDIA SOSIAL INSTAGRAM TERHADAP MINAT BELI KONSUMEN JASA MAKEUP ARTIS

This study aims to find out about promotions on Instagram @imdproject whether the Promotion variable (X) affects Consumer Purchase Interest on Instagram social media @imdproject (Y). The population in this study were customers who had ordered makeup artist services on social media Instagram @imdproject with a population of 97 followers using the Non-Probability Sampling technique, saturated sample method with a quantitative approach. Using a questionnaire/questionnaire data collection technique, the analysis technique uses a 5-point Likert scale. Data analysis techniques using instrument analysis testing research, classical assumptions, and hypothesis testing with the help of the SPSS application program version 26. And the results of this study note that the promotion variable (X1) has a significance value of 0.000 with a beta value of unstandardized coefficients of 0.494. These results indicate that the significance value is less than 0.05, which means that H1 supported and H0 rejected. Based on these results, it can be concluded that the promotion variable (X1) is stated to have a positive and significant effect on the purchase intention variable (Y).

Sympathetic Nervous Influences Are Negative Prognostic Factors in Stomach Cancer.

(1) Background: The aim of our study was to assess the involvement of the sympathetic nervous system in the progression of patients with gastric carcinoma by analyzing the sympathetic neuronal fibers and beta 2 adrenoreceptors. (2) Methods: We performed a retrospective study in which we analyzed the clinical, biological, and histological data from a total of 104 patients diagnosed with stomach cancer. (3) Results: After analyzing the immunoreactivity of beta 2 adrenoreceptors, we observed increased values in patients with tumors larger than 5 cm in diameter (p = 0.0371), with a deeper degree of tumor invasion T3-4 (p = 0.0159), invasion in more than two lymph nodes (p = 0.0462), or a TNM stage III-IV. Regarding the survival analysis, better survival rates (65%) were observed for patients with a low value of beta 2 adrenoreceptors (B2A-), compared to B2A (+) patients, in which survival at 3 years of follow-up was only 43%. In addition, the analysis of intra-tumoral sympathetic fibers showed a better survival rate (83%) for patients with a low value of density compared to patients with increased density, in whom the survival rate was only 24%. (4) Conclusions: The findings of this study indicate that patients with stomach cancer have a more unfavorable prognosis when they have a higher density of sympathetic nerve fibers and an increased expression of beta 2 adrenergic receptors inside the tumor.

Elucidating drivers of repurchase intention in the e-marketplace through the lens of online trust-building mechanisms

Indonesia has low e-commerce transactions despite high internet usage. This study examines the e-repurchase intention on Lazada Indonesia, an e-marketplace with declining traffic and sales. This study uses the perceived usefulness of institutional-based mechanisms, the perceived usefulness of seller-based mechanisms, and the perceived usefulness of experience-based mechanisms to examine how trust in the e-market and e-seller affect repurchase intention. This quantitative study includes 231 Lazada Indonesia customers from the past three months (the survey was conducted in January 2023). The data were statistically analyzed with partial least squares structural equation modeling (PLS-SEM). 43.72% of the respondents shop one to three times a month, 42.42% – more than three times per month, and 13.85 – less than once per month. Trust in the e-marketplace increased when participants believed institutional-based processes were beneficial (with a beta value of 0.272 and a P value of 0.000) and seller-based mechanisms were valuable (with a beta value of 0.509 and a P value of 0.000). In terms of trust in the e-seller, only the perceived usefulness of seller-based mechanisms has a significant effect (with a beta value of 0.567 and a P value of 0.000), while the perceived usefulness of experience-based mechanisms has no effect. This study has also shown that e-seller trust significantly affects repurchase intention. Finally, with a beta value of -0.055 and a P value of 0.046, e-marketplace trust negatively moderates the relationship between e-seller trust and repurchase intention. Thus, e-marketplace trust can replace e-seller trust in customer repurchase intentions. AcknowledgmentThis study is conducted with the support of Universitas Multimedia Nusantara.

(060) Epigenetic and Gene Expression Profiles in Non-Plaque Tissues from Men with Peyronie’s Disease

Abstract Introduction Peyronie’s Disease (PD) can significantly impact sexual function. A hereditary predisposition to PD suggests that genetic variants may exist, and gene expression may modulate the penetrance of the disease. Identification of changes in epigenetic and gene expression profiles will facilitate a deeper understanding of the disease and provide insight for more targeted therapies. Objective Our objective is to determine if PD is correlated with epigenetic variations and/or gene expression changes, as indicated by DNA methylation assessment and RNA sequencing. Methods Penile tunica albuginea samples (not from fibrotic plaques) were obtained from men with PD undergoing penile prosthesis placement. Control samples were collected from men with erectile dysfunction (ED) without PD undergoing penile prosthesis placement. DNA methylation analyses were performed on homogenized penile tissue samples via an Illumina Human MethylationEPIC BeadChip v2 array. Using the minfi package in R, beta values were produced for all 936,990 CpG sites for each sample and SWAN normalization was applied. Differentially methylated regions (DMR) were found via USEQ with a threshold Wilcoxon FDR score of 40. Gene expression levels were examined in PD and ED samples via RNA-sequencing. Gene expression data was compared against epigenetic data to determine if any epigenetics changes identified affected gene expression profiles. Results A total of 24 tissue samples were collected: 12 ED and 12 PD. USEQ yielded 36 total DMRs with an FDR score of 40 or greater (p-value&amp;lt;0.0001). Of these 36 DMRs, a Stanford GREAT analysis revealed 60 total gene-region associations and five implicated biological processes: anterior/posterior pattern specification, chordate embryonic development, embryo development (ending in birth or egg hatching), somitogenesis, and pattern specification process. Furthermore, differential gene expression analysis identified 76 genes with significant expression changes between PD and ED groups, of which 17 genes were upregulated while 59 were downregulated. Long noncoding RNAs and the keratocan gene showed notable expression differences between PD and ED groups. GO enrichment analysis revealed significant terms related to DNA binding and protein binding. None of the differentially regulated genes overlapped with identified DMRs. Conclusions This study identified distinct gene expression and DNA methylation patterns between PD and ED groups, despite PD samples not being taken from plaque regions, suggesting that more systemic changes may be present. Upregulated genes in PD included long noncoding RNAs and the keratocan gene, suggesting their involvement in disease pathogenesis. Long noncoding RNA overexpression may serve a regulatory function in gene expression or cell processes while overexpression of the keratocan gene may promote collagen accumulation and remodeling, leading to fibrous plaque formation. Likewise, DMRs and their region-gene associations suggest a role in early embryonic development for pathogenesis. Although RNA-seq did not indicate any significant differentially expressed genes that matched with gene-region associations correlated with DMRs, the significance and quantity of DMRs between ED and PD sample groups is indicative of affected biological processes that manifest in distinct pathophysiologies. Further research is needed to investigate if differentially regulated genes exist in DMRs that exhibited changes but did not meet the criteria threshold for inclusion here. Disclosure Any of the authors act as a consultant, employee or shareholder of an industry for: Contraline, Techfields Inc, Alto Neurosciences, Curebase.

Caregiver burden and health-related quality of life: A study of informal caregivers of older adults in Ghana.

Similar to many developing countries, caregiver burden remains high in Ghana which may affect informal caregivers of older adults' health-related quality of life (HRQoL). However, no study has examined the association between caregiver burden and HRQoL among informal caregivers of older adults in Ghanato date. Understanding this association may well help to inform health and social policy measures to improve HRQoL among informal caregivers of older adults in Ghana. Situated within a conceptual model of HRQoL, the purpose of this study was to examine the relationship between caregiver burden and HRQoL among informal caregivers of older adults in Ghana. We obtained cross-sectional data from informal caregiving, health, and healthcare (N = 1853) survey conducted between July and September 2022 among caregivers (≥ 18 years) of older adults (≥ 50years)inthe Ashanti Region of Ghana. The World Health Organization Impact of Caregiving Scale was usedto measure caregiver burden. An 8-item short form Health Survey scale developed by the RAND Corporation and the Medical Outcomes Study was used to measure HRQoL. Generalized Linear Models were employed to estimate the association between caregiver burden and HRQoL. Beta values and standard errors were reported with a significance level of 0.05 or less. The mean age of the informal caregivers was 39.15years and that of the care recipients was 75.08years. In our final model, the results showed that caregiver burden was negatively associated with HRQoL (β = - .286, SE = .0123, p value = 0.001). In line with the conceptual model of HRQoL, we also found that socio-economic, cultural, demographic and healthcare factors were significantly associated with HRQoL. For instance, participants withno formal education (β = -1.204, SE=.4085, p value =0.01), those withprimary level of education (β = -2.390, SE=.5099, p value =0.001) orjunior high school education (β = -1.113, SE=.3903, p value= 0.01) hada significantly decreased HRQoLcompared to those with tertiary level of education. Participants who werebetween the ages of 18-24 (β = 2.960, SE=.6306, p value=0.001), 25-34 (β = 1.728, SE=.5794, p value=0.01) or 35-44 (β = 1.604, SE=.5764, p value= 0.01) years significantly had increased HRQoLcompared to those who were 65 years or above. Also, participants who did not utilize healthcare services in the pastyear before the survey significantly had increased HRQoLcompared to those who utilized healthcare services five or more times in the pastyear (β = 4.786, SE=. 4610, p value=0.001). Consistent with our hypothesis, this study reported asignificant negativeassociation between caregiver burden and HRQoL. Our findings partially support the conceptual model of HRQoLused in this study. We recommend that health and social policy measures to improve HRQoL among informal caregivers of older adultsshould consider caregiver burden as well as other significant socio-economic, cultural, demographic, and healthcare factors.

The Intraday Heterogeneity and Risk Pricing Reversal Between Day and Night: Evidence from China

ABSTRACT Using a large panel of high-frequency trading data, this article investigates the intraday heterogeneity of risk pricing process in the Chinese stock market. Our research reveals that the financial assets in China exhibit intraday beta variation: high/low market risk level assets, with beta values above/below unity, tend to have downward/upward sloping beta trajectories across the intraday trading periods. We confirm a trend reversal of the risk-return relation between day and night in the Chinese market: the assets’ intraday returns are negatively correlated with their market risk level, whereas their overnight returns are positively correlated with their risk level. Further investigation shows a connection between the intraday beta variation and the day-night reversal anomaly, and the risk-return reversal between day and night can be partially explained by the intraday beta variation of financial assets. After controlling for the intraday beta variation, the risk-return reversal anomaly between day and night becomes less pronounced in the Chinese market.

Impact of Artificial Intelligence (AI) on Selected Human Resource Management (HRM) Functions in Pharmaceutical Industry in India

The study aims to assess artificial intelligence's effects on select HRM functions and to validate the proposed conceptual framework through empirical analysis showing the connection between HRM function as well as artificial intelligence. Questionnaire consisting of closed-ended questionnaires were distributed in order to achieve these goals. The reliability, validity, and correlation analysis were used to assess the factors that make up the suggested model. The hypothesis was further tested and the suggested model was validated using regression analysis. Findings show that all variables account for 89% of HRM explanation, with a R square (R2) of 0.890. The ANOVA values for the regression model, indicate validation at a 95% confidence level. The beta (β) values of all factors are 0.902 and 0.545 in the coefficient summary, which is a reasonable representation of their influence on HRM. These strong AI-based HR apps are a valuable tool for any type of business, even though they lack cognitive capacities of humans. This study will help most businesses to successfully integrate AI-related techniques into hiring, according to our research, as AI will permeate every aspect of HR in the near future and should be seen as a good thing since it makes life better.

(059) Epigenetic Profiles of Men with Peyronie’s Disease

Abstract Introduction Peyronie’s Disease (PD) can significantly impact sexual function. A hereditary predisposition to PD suggests that genetic variants may exist, and gene expression may modulate the penetrance of the disease. Identification of changes in epigenetic or gene expression profiles would facilitate a deeper understanding of the disease and provide insight for more targeted therapies. Objective Our objective is to determine if predisposition to PD is correlated with epigenetic variations and/or gene expression changes, as indicated by DNA methylation assessment and RNA sequencing. Methods Penile tunica albuginea samples were obtained from men with PD undergoing penile prosthesis placement. Penile sample from these men were not from fibrotic plaques. Control samples were collected from men with erectile dysfunction (ED) without PD undergoing penile prosthesis placement. DNA methylation analyses were performed on homogenized penile tissue samples via an Illumina Human MethylationEPIC BeadChip v2 array. Using the minfi package in R, beta values were produced for all 936,990 CpG sites for each sample and SWAN normalization was applied. Differentially methylated regions (DMR) were found via USEQ with a threshold Wilcoxon FDR score of 40. Gene expression levels were examined in PD and ED samples via RNA-sequencing. Gene expression data was compared against epigenetic data to determine if any epigenetics changes identified affected gene expression profiles. Results A total of 24 tissue samples were collected: 12 ED and 12 PD. USEQ yielded 36 total DMRs with a FDR score of 40 or greater (p-value&amp;lt;0.0001). Of these 36 DMRs, a Stanford GREAT analysis revealed 60 total gene-region associations and five implicated biological processes: anterior/posterior pattern specification, chordate embryonic development, embryo development (ending in birth or egg hatching), somitogenesis, and pattern specification process. Furthermore, differential gene expression analysis identified 76 genes with significant expression changes between PD and ED groups, of which 17 genes were upregulated while 59 were downregulated. Long noncoding RNAs and the keratocan gene showed notable expression differences between PD and ED groups. GO enrichment analysis revealed significant terms related to DNA binding and protein binding. None of the differentially regulated genes overlapped with identified DMRs. Conclusions This study identified distinct gene expression and DNA methylation patterns between PD and ED groups, despite PD samples not being taken from plaque regions. Upregulated genes in PD included long noncoding RNAs and the keratocan gene, suggesting their involvement in disease pathogenesis. Long noncoding RNA overexpression may serve a regulatory function in gene expression or cell processes while overexpression of the keratocan gene may promote collagen accumulation and remodeling, leading to fibrous plaque formation in the affected area. Likewise, DMRs and their region-gene associations suggest a role in early embryonic development for pathogenesis. Although RNA-seq did not indicate any significant differentially expressed genes that matched with gene-region associations correlated with DMRs, the significance and quantity of DMRs between ED and PD sample groups is indicative of affected biological processes that manifest in distinct pathophysiologies. Further research is needed to investigate if differentially regulated genes exist in DMRs that exhibited changes but did not meet the criteria threshold for inclusion here. Disclosure Any of the authors act as a consultant, employee or shareholder of an industry for: Paterna bio, Firmtech, Turtle health, Maximus, Carrot, Contraline, endo pharmaceuticals, inherent biosciences, techfields inc, macro trials, alto neurosciences, curebase, vivify medical.