Macropus Rufogriseus Research Articles

No distinct stratification of ingesta particles and no distinct moisture gradient in the fore-stomach of non-ruminants: The wallaby, peccary, hippopotamus, and sloth

Herbivores that digest plant material in the fore-stomach can be divided in ruminants and non-ruminants. This study describes the distribution of feed particles (and inorganic material) and dry matter (DM) in the digestive tract of non-ruminant foregut fermenters. Results from passage trials led us to hypothesize that specific particle-sorting mechanisms, as observed in ruminants, are unlikely in non-ruminants. Therefore, no systematic particle size distribution effects (indicative of a sorting mechanism) should be evident in the fore-stomachs of these animals, but differences in fluid and particle retention suggest that differences in fluid concentration (measured as DM) could occur in the foregut of macropods and hippos. The gut content of eleven Bennett's wallabies (Macropus rufogriseus), six collared peccaries (Pecari tajacu), three pygmy hippos (Hexaprotodon liberiensis), two common hippos (Hippopotamus amphibius) and one two-toed sloth (Choloepus didactylus) were analyzed with an emphasis on the fore-stomach. The ventral and dorsal regions in sacciform compartments, and peripheral and central regions in tubular compartments, were examined. Results were not uniform across the species studied. A potential sedimentation mechanism was observed firstly by the accumulation of sand in the fore-stomach of the peccary and sloth, and secondly by the lower DM content in peripheral versus central and ventral versus dorsal regions of the fore-stomach of the wallabies and common hippos, respectively. However, pair-comparisons for different gut regions of wallabies and peccaries yielded no differences in mean particle size between fore-stomach regions. To conclude, some digesta fractionation does occur in the fore-stomach of the studied groups of non-ruminants, but not in a uniform manner, which in turn is in accordance with morphological dissimilarities of their respective foregut structures. The absence of systematic fractionation effects in non-ruminant foregut fermenters emphasizes the innovative character of the sorting mechanism in ruminants.

Fatal toxoplasmosis associated with an atypical Toxoplasma gondii strain in a Bennett's wallaby (Macropus rufogriseus) in Spain

Toxoplasmosis is often fatal in captive wallabies, but the causes of this high susceptibility are not well understood. Here, we report fatal toxoplasmosis in a Bennet's wallaby (Macropus rufogriseus) due to an atypical Toxoplasma gondii strain for the first time in Europe. The wallaby was from a colony of 7 Bennet's wallabies that died over a 17-month period at a safari-zoological park in northeastern Spain. Only one of these wallabies was examined at necropsy. T. gondii-like organisms were detected by histological examination in several tissues and the diagnosis was confirmed through detection of T. gondii DNA by PCR. A nested PCR-based assay detected the 200- to 300-fold repetitive 529bp DNA fragment of T. gondii in a sample of brain tissue. Genotyping analysis with 15 single-copy microsatellite markers was performed on this positive DNA sample and revealed an atypical genotype. Atypical genotypes are frequently associated with severe forms of toxoplasmosis in humans. The present report highlights the possible implications of the introduction of new atypical, more pathogenic T. gondii strains, to non-endemic areas.

The success of GPS collar deployments on mammals in Australia

Global Positioning System (GPS) wildlife telemetry collars are being used increasingly to understand the movement patterns of wild mammals. However, there are few published studies on which to gauge their general utility and success. This paper highlights issues faced by some of the first researchers to use GPS technology for terrestrial mammal tracking in Australia. Our collated data cover 24 studies where GPS collars were used in 280 deployments on 13 species, including dingoes or other wild dogs (Canis lupus dingo and hybrids), cats (Felis catus), foxes (Vulpes vulpes), kangaroos (Macropus giganteus), koalas (Phascolarctos cinereus), livestock guardian dogs (C. l. familiaris), pademelons (Thylogale billardierii), possums (Trichosurus cunninghami), quolls (Dasyurus geoffroii and D. maculatus), wallabies (Macropus rufogriseus and Petrogale lateralis), and wombats (Vombatus ursinus). Common problems encountered were associated with collar design, the GPS, VHF and timed-release components, and unforseen costs in retrieving and refurbishing collars. We discuss the implications of collar failures for research programs and animal welfare, and suggest how these could be avoided or improved. Our intention is to provide constructive advice so that researchers and manufacturers can make informed decisions about using this technology, and maximise the many benefits of GPS while reducing the risks.

PASTEURELLA MULTOCIDACARRIAGE IN RED-NECKED WALLABIES (MACROPUS RUFOGRISEUS)

The isolation of Pasteurella multocida from several red-necked wallabies (Macropus rufogriseus) with purulent to necrotizing gingivitis, mandibular osteomyelitis, and conjunctivitis in a private zoological park prompted a cross-sectional prevalence study in two zoological collections. The study demonstrated a high prevalence of P. multocida carriage in clinically healthy red-necked wallabies. In one collection (n= 7), P. multocida was isolated from the gingival mucosa of two animals (28.6%) and the conjunctiva of one animal (14.3%). In another collection (n = 29), P. multocida was isolated from the buccal mucosa in two animals (6.9%) and the pharyngeal mucosa of eight animals (27.6%). Multilocus sequence typing showed that sequence types varied among, but were identical within, collections.

Redescriptions and descriptions of new species of Austrostrongylus Chandler, 1924 (Nematoda: Trichostrongylina), from Australian marsupials with a comparative study of features of the synlophe

Redescriptions are provided of Austrostrongylus aggregatus Johnston & Mawson, 1940, A. chandleri Mawson, 1973, A.macropodis Chandler, 1924, A. victoriensis Cassone, 1983 and A. wallabiae Johnston & Mawson, 1939 from macropodidmarsupials from eastern Australia. Two new species are described: A. mawsonae sp. nov. from Wallabia bicolor(Desmarest) and A. smalesae sp. nov. from Macropus rufogriseus (Desmarest), M. fuliginosus (Desmarest), M. giganteusShaw and W. bicolor (Desmarest). A. paratypicus Mawson, 1973 is treated as a synonym of A. macropodis. The synlopheis described from serial transverse sections of males and females of each species and the origins and terminations of thecuticular ridges documented for use in specific identification. Females of some species could be distinguished only bydifferences in the synlophe. The geographical and host ranges of all know species are expanded based on newercollections. Based on a study of seven species, comments are provided on the remaining congeners. A. petrogaleBeveridge & Durette-Desset, 1986 and A. safestatus Beveridge & Durette-Desset, 1986 are removed to the genusSutarostrongylus Beveridge & Durette-Desset, 1986 as S. petrogale comb. nov. and S. safestatus comb. nov. Keys are provided to males based on the features of the spicules and to females based on features of the synlophe.

Minimising number killed in long-term vertebrate pest management programmes, and associated economic incentives

AbstractManagement of invasive vertebrate species often requires the use of lethal control tools such as toxins, traps, or shooting. However, because these pest species are sentient and have the capacity to suffer, the application of such tools raises concerns about welfare impacts. To address such concerns, research, policy and regulation have focused most often on the welfare impacts (humaneness) of the tools at the individual animal level (ie the ‘quality’ of the impact) with no attempt to assess welfare at the population level (ie the ‘quantity’ of the impact). Because control programmes often target large numbers of animals, we suggest that when the welfare costs of pest control operations and strategies are being evaluated, the numbers of individuals involved should be considered in addition to the intensity and duration of individual suffering. We explore this concept using a modelling framework and three New Zealand case studies (brushtail possums [Trichosurus vulpecula], ship rats[Rattus rattus], and Bennett's wallabies[Macropus rufogriseus])to assess the extent to which typical control strategies used by land managers influence the numbers of animals killed. We test whether a predicted relationship between numbers killed and position on the population growth curve holds across these scenarios, and identify whether it would be economically viable for end-users to adopt more welfare-friendly control strategies (ie those that kill fewer individuals to achieve the required management outcomes) for these pest species, or whether some form of incentive would be required. Computer modelling showed that for four simulated brushtail possum control strategies, the number of animals killed on a 1,000-ha area over 30 years ranged from approximately 13,000 to 26,000. Similarly, for two ship rat control strategies, numbers killed over a 20-year period were 977 for an aerial strategy versus 1,517 for a ground-based strategy. For both species, the strategies that killed fewest animals generally also cost the least. For Bennett's wallabies, because farmers only carry out control for production benefits, the control strategy they are most likely to select would result in the highest number of wallabies killed. To reduce the number of wallabies killed while allowing farmers to achieve some production benefits, farmers would need to receive some additional financial benefit. The concept of welfare incentive then raises questions such as ‘what willingness is there to pay for increased welfare’ and ‘to what extent can reducing control costs substitute for incentive payments in reducing numbers killed?’

Methane production by red-necked wallabies (Macropus rufogriseus)

The claimed low production of CH(4) by kangaroos and marsupials in general has been questioned because of a lack of data. The extent of their CH(4) production is of interest both from the point of view of discussing meat production of marsupials and as a basis for developing methods to reduce CH(4) production from ruminants. In the present experiment, the CH(4) production of 8 red-necked wallabies (Macropus rufogriseus) was measured of which 4 were fed 2 different diets in an open-circuit respiration chamber. These results were compared with a newly developed, inexpensive, and simple method that does not influence the behavior of the animal, and where the ratio between CH(4) and CO(2) is measured and used together with the calculated CO(2) to quantify the CH(4) production. The experiment demonstrated that the wallabies produce CH(4). However, the amount of CH(4) produced by these wallabies was between 1.6 and 2.5 L/d equivalent to 1.6 and 2.5% of GE or 2.2% and 3.5% of DE intake and 0.22 L/BW, kg(0.75). This is between 25 and 33% of what can be expected from ruminants fed the same diet. Based on the uneven release of CH(4) with time, it is most likely that the CH(4) is excreted through flatulence and not through breathing as is seen in ruminants. The experiments also showed that a reasonably accurate determination of the CH(4) production of a group of animals can be obtained by simply measuring the CH(4)/CO(2) ratio over a limited time span. This may represent the situation in a natural setting better than measurements in a respiration chamber. It was found that the CH(4)/CO(2) ratio in itself represents a reasonable prediction of the proportion of feed GE that is lost as CH(4), and that this method offers new opportunities for CH(4) measurements on a large number of animals.

Effects of wildlife grazing on the production, ground cover and plant species composition of an established perennial pasture in the Midlands region, Tasmania

Context Management of grazing wildlife on private land in Tasmania is a contentious issue for landowners, animal-welfare groups and the Tasmanian Government. Wildlife species known to graze pasture include Tasmanian pademelon (Thylogale billardierii), Bennett’s wallaby (Macropus rufogriseus rufogriseus), forester kangaroo (Macropus giganteus), brushtail possum (Trichosurus vulpecula) and fallow deer (Dama dama). Understanding the spatio-temporal patterns of wildlife grazing is important when considering wildlife-control options to mitigate pasture loss; however, limited research has been undertaken. Aims To quantify the impact of wildlife grazing on pasture production and to assess the spatial and temporal pasture biomass loss from an established pasture; to investigate the effect of protecting pastures from wildlife grazing on species composition of an existing perennial pasture; to determine whether wildlife grazing contributes to a decline in the composition of improved pasture species over time and an increase in-ground cover of less desirable grasses and broadleaf weeds; and to examine whether protecting pastures from wildlife grazing could increase ground cover. Methods Pasture biomass loss to wildlife grazing was determined by a paired exclusion-cage method over a 26-month period from February 2008 to April 2010. A quantitative pasture model was used to simulate pasture growth at the study site. Changes in the botanical composition of the sward in response to wildlife grazing were determined by hand-separation, drying and weighing of harvested material, and also by visual estimation of the ground cover of individual plant species. A wildlife faecal-pellet survey was used to develop an index of wildlife feeding activity. Key results Pasture loss to wildlife grazing varied spatially and temporally. Pasture loss decreased with increasing distance from the edge of cover vegetation. The proportion of pasture lost increased during periods of slow pasture growth. Visual estimates of ground cover showed that grazing by wildlife resulted in an increase in bare ground in unprotected swards, whereas protection from grazing resulted in an increase in production of perennial and annual species, as determined by hand-separation of harvested material, and a decrease in bare ground as determined by visual estimate. Faecal-pellet surveys were found to be strongly correlated with pasture biomass losses. Conclusions The proportion of pasture loss to wildlife grazing was found to be influenced by distance from native vegetation and also by pasture availability, which was seasonal. Wildlife can alter the composition of pastures by reducing the ground cover and yield of improved grasses. Continual grazing of pastures by wildlife in addition to rotational sheep grazing may increase the amount of bare ground. Implications Wildlife-control methods need to be carefully chosen if the intended benefits of alleviating pasture biomass losses are to be achieved. Quantifying the loss of pasture is important because it enables the extent and significance of losses to be determined and may inform decisions about the most appropriate wildlife control measures to adopt. Controlling wildlife during periods of slow pasture growth may be important in preventing damage and yield loss of plant species actively growing during these times. Failure to control wildlife may result in a decrease in the composition of desirable plant species.

Limb preferences in a marsupial, Macropus rufogriseus: evidence for postural effect

In many primate species, bipedal stance is a factor increasing manual laterality. To understand this phenomenon better, there is a need to investigate forelimb preferences in nonprimate mammals with bipedal locomotion as the preferred gait, such as bipedal hopping marsupials. We studied laterality in forelimb use in 27 adult red-necked wallabies during their usual daily activity in five zoos. During feeding from the bipedal position adult wallabies displayed group-level left-forelimb preference with the majority of individuals being lateralized. However, no lateralization was found at the group level during feeding from the quadrupedal position, with only a few animals expressing individual preferences. Wallabies showed significant group-level bias while using the right forelimb for supporting the body in the tripedal stance, with nearly half the individuals being lateralized. On a smaller sample of nine wallabies forelimb preferences in unimanual snout autogrooming were studied: seven individuals preferred to use their left forelimb and two displayed no preference. Additionally, we studied asymmetrical limb use during milk suckling in six young-at-foot wallabies. Here, all subjects more often pulled down the mother’s pouch edge with the left forelimb and simultaneously supported the body with the right paw. Our results show that marsupials can display motor laterality at both individual and group levels, comparable with those observed in placentals. Moreover, in red-necked wallabies, much as in primates, the bipedal posture favours the expression of manual laterality, while its direction depends on the functional nature of the motor action. Evolutionary scenarios such as the postural origin theory of behavioural lateralization are discussed.

Evaluation of Medetomidine-Alfaxalone and Medetomidine-Ketamine in Semi-Free Ranging Bennett's Wallabies (Macropus rufogriseus)

Twenty-six adult semi-free-ranging Bennett's wallabies were anesthetized. Animals in group MA received medetomidine 0.1 mg/kg and alfaxalone 4 mg/kg i.m. in a 5-ml dart, whereas those in group MK received medetomidine 0.1 mg/kg and ketamine 5 mg/kg i.m. in a 3-ml dart. Dosages were based on estimated body weights. The wallabies were allowed to recover spontaneously or, if still nonresponsive at the end of the procedure, were given atipamezole 0.5 mg/kg (half the dose via i.m. and the other half via i.v.). Heart rate and respiratory rate were monitored at 5-min intervals, temperature at 10-min intervals, and two arterial blood samples were taken for blood gas analysis. Statistical analysis was performed by using analysis of variance (P < 0.05). The use of 5-ml darts in group MA compared with 3-ml darts in group MK could potentially increase the risk of iatrogenic trauma and should be considered. Induction and maintenance of anesthesia were satisfactory in both groups. There were no significant differences between the groups in mean time to first effect, recumbency, and approach, or to time to sternal recumbency and standing after reversal with atipamezole. Although bradycardia was present in both groups, no statistical differences were calculated for respiratory rate and heart rate, whereas the mean cloacal temperature was significantly lower in group MA (P = 0.01). Mixed acid-base disturbances occurred in both groups. All but one animal in group MK needed atipamezole at the end of the procedure. No adverse effects were observed after recovery.

Immunome database for marsupials and monotremes

BackgroundTo understand the evolutionary origins of our own immune system, we need to characterise the immune system of our distant relatives, the marsupials and monotremes. The recent sequencing of the genomes of two marsupials (opossum and tammar wallaby) and a monotreme (platypus) provides an opportunity to characterise the immune gene repertoires of these model organisms. This was required as many genes involved in immunity evolve rapidly and fail to be detected by automated gene annotation pipelines.DescriptionWe have developed a database of immune genes from the tammar wallaby, red-necked wallaby, northern brown bandicoot, brush-tail possum, opossum, echidna and platypus. The resource contains 2,235 newly identified sequences and 3,197 sequences which had been described previously. This comprehensive dataset was built from a variety of sources, including EST projects and expert-curated gene predictions generated through a variety of methods including chained-BLAST and sensitive HMMER searches. To facilitate systems-based research we have grouped sequences based on broad Gene Ontology categories as well as by specific functional immune groups. Sequences can be extracted by keyword, gene name, protein domain and organism name. Users can also search the database using BLAST.ConclusionThe Immunome Database for Marsupials and Monotremes (IDMM) is a comprehensive database of all known marsupial and monotreme immune genes. It provides a single point of reference for genomic and transcriptomic datasets. Data from other marsupial and monotreme species will be added to the database as it become available. This resource will be utilized by marsupial and monotreme immunologists as well as researchers interested in the evolution of mammalian immunity.

Effectiveness of cyanide pellets for control of Bennett's wallaby (Macropus rufogriseus rufogriseus) in New Zealand

Bennett's wallaby (Macropus rufogriseus rufogriseus) are an introduced pest requiring control in New Zealand. Historically, sodium fluoroacetate (1080) has been used, but there is political resistance to this method of control from specific interest groups. Previous trials with captive animals have shown that Feratox® cyanide pellets are efficacious for Bennett's wallaby and the aim of this study was to field test Feratox®. A 300-ha treatment block was pre-fed non-toxic bait for 2 weeks followed by the presentation of 200-mg Feratox® pellets. Rates of faecal pellet accumulation were markedly higher in the non-treatment block following control and the Guildford score of wallaby abundance reduced from 5 to 2 in the treatment block. In total, 110 dead wallabies were found less than 50 m from the baiting sites. These results suggest that Feratox® is an effective ground-based alternative to 1080 for Bennett's wallaby control.

First Description of Onychomycosis Caused by Chrysosporium keratinophilum in Captive Bennett's Wallabies (Macropus rufogriseus rufogriseus)

Seven Bennett's wallabies (Macropus rufogriseus rufogriseus) presented within a period of several months with onychodystrophy, onychomadesis, and severe digital tumefaction. Histopathologic findings included a pseudocarcinomatous hyperplasia of the claw matrix surrounding a cavity filled with keratin and septate hyphae stained with periodic acid Schiff reagent. The fungal species Chrysosporium keratinophilum was identified on cultures. The wallabies were orally treated with ketoconazole (15 mg/kg s.i.d.) for 20 wk. Material and enclosures were cleaned and sprayed with 0.2% enilconazole solution once a month over a period of 4 mo. No improvement of advanced cases was observed, but no new case appeared for the next 6 mo. The positive mycological culture and the invasion of tissues on histopathologic examination suggested that the fungal species C. keratinophilum was implicated in this claw disease. This is the first report of onychomycosis caused by C. keratinophilum in animals.

Is the altitudinal distribution of the Broad-toothed rat in Tasmania a result of the effects of Bennett's Wallaby?

The Broad-toothed Rat Mastacomys fuscus is infrequently recorded in Tasmania and was only recently discovered at altitudes >1000 m, although the species occurs on the Australian mainland at >2200 m. A survey of Tasmanian alpine habitat found evidence of M. fuscus within a metre or two of the summit of Cradle Mountain at 1545 m, the highest altitude recorded for M. fuscus within Tasmania. There was a negative relationship between the presence of Bennett's Wallaby (Macropus rufogriseus) and M. fuscus at all sites. This is analogous to the absence of tall alpine herbfield as a plant community in Tasmania as a result of grazing by M. rufogriseus. At Cradle Mountain, M. fuscus occurred in alpine areas above rock barriers that prevented access by M. rufogriseus and hence allowed sufficient growth of grass to support populations of M. fuscus.This study further demonstrates the importance of M. rufogriseus in alpine ecosystems that lack a seasonal snow cover and raises the question of the potential impacts of M. ...

Suitability of acoustics as non-lethal deterrents for macropodids: the influence of origin, delivery and anti-predator behaviour

Context Auditory devices used to deter wildlife are a potentially humane and effective way of minimising deleterious interactions with humans and their livelihoods and have been used successfully for many species around the world. Acoustic cues can be used to manipulate anti-predator behaviour, encouraging animals to forage elsewhere. Employing acoustics derived from natural sources to make use of innate behavioural responses has been suggested to outperform novel or artificial sounds; however, anti-predator strategies vary among sympatric species and will influence the utility of acoustic stimuli for deterring wildlife. Aims We aimed to test the interaction between the source of origin (natural or novel) and species traits (anti-predator strategy – grouping behaviour) on the efficacy of using acoustic stimuli to elicit alarm responses for two species in the family Macropodidae commonly associated with browsing on forest plantation seedlings; the red-necked pademelon (Thylogale thetis) and the red-necked wallaby (Macropus rufogriseus banksianus). Methods We tested these factors in captivity using playback experiments of acoustic stimuli and monitored the behavioural responses of subjects. Results Red-necked pademelons exhibited strong responses to bioacoustic and novel stimuli but did not greatly differentiate among them. Short-term habituation to predator calls was detected whereas responsiveness to novel sounds increased. Red-necked wallabies most strongly responded to conspecific distress calls, showing no sign of short-term habituation. Conclusions Results from the present and other studies suggest that bioacoustic deterrents, particularly those utilising natural conspecific sounds, aimed at communicating danger, have the potential to play an important role in non-lethal wildlife management, although that responsiveness varies with the form of anti-predator strategies employed. Implications If alarm responses translate into subjects vacating targeted areas, then there is some potential to implement non-lethal acoustic deterrents for macropodids alongside other management measures aimed at preventing impacts on primary production. Problematically, our experiments showed that without accurate acoustic delivery, particularly of sounds with infrasonic components, the development of effective bioacoustic deterrents may remain stymied.

Terrestrial mammals of a sheep-grazing property on Bruny Island, Tasmania

Land-based mammals were surveyed in a mosaic of dry sclerophyll forests and pasture on a sheep-grazing property on Bruny Island, Tasmania, using a range of methods in August 2010. This is the first mammal survey of a sheep-grazing property in Tasmania and the first large-scale survey of mammals on Bruny Island. Ten species were recorded comprising seven native and three introduced species. The Little Forest Bat, Vespadelus vulturnus , and the Black Rat, Rattus rattus , were recorded for the first time on Bruny Island, although both are probably long-term residents. No mammal species listed as rare or threatened under Tasmanian or Australian legislation were found on the property. Large numbers of Eastern Quolls, Dasyurus viverrinus , Brushtail Possums, Trichosurus vulpecula , Tasmanian Pademelons, Thylogale billardierii , and Bennetts Wallabies, Macropus rufogriseus , were recorded in a range of dry sclerophyll forests and in pasture. Longnosed Potoroos, Potorous tridactylus , were recorded widely on the property in native vegetation with relatively thick ground cover. Eastern Quoll capture rates were highest in pasture areas and in Eucalyptus ovata forest. Brushtail Possums, Long-nosed Potoroos, Tasmanian Pademelons and Bennetts Wallabies were virtually unrecorded from E. tenuiramis forest and woodlands. Given the level of survey effort and their potential to occur on the property it was remarkable that no Tasmanian Bettong, Bettongia gaimardi , Eastern Barred Bandicoot, Perameles gunnii , Southern Brown Bandicoot, Isoodon obesulus , or introduced House Mouse, Mus musculus , were recorded. We found that camera trapping was more cost-efficient than cage trapping for detecting the presence of mammals on Murrayfield. Recommendations for ongoing management and monitoring of mammals are provided.

Sperm motility activation, sperm heterogeneity and sperm–female tract interactions in Bennett's wallaby (Macropus rufogriseus rufogriseus)

Sperm-oviduct interactions in Bennett's wallaby (Macropus rufogriseus rufogriseus) were investigated using in vitro cocultures of cauda epididymal spermatozoa and oviducal epithelial cells. Kidney epithelial cells were used as non-reproductive control tissues. Spermatozoa attached to epithelial cells of both origins, but sperm survival and activity was higher when cocultured with oviducal cells. New findings during live sperm-epithelial interactions included: (1) a high frequency of reversible head movements, from linear (streamlined configuration) to T shape (thumbtack configuration) in swimming spermatozoa immediately after the start of coculture; (2) the loss of sperm tails (tail shedding) increasing with time; and (3) interrupted swimming patterns, where periods of fast movement were interspersed with slower swimming while the spermatozoa interacted with the epithelial cell surface. Sperm motility activation responses were characterised after diluting the epididymal samples in phosphate-buffered saline, medium M199 and Tyrode's medium. The results confirmed that the marsupial oviduct is able to support the viability and motility of a sperm subpopulation for at least 20 h in vitro and suggest that some spermatozoa shed their tails after binding, possibly as a result of a selective process.

Macropod habitat use and response to management interventions in an agricultural - forest mosaic in north-eastern Tasmania as inferred by scat surveys

Context Native pest herbivores often require population numbers to be controlled in landscape settings where agricultural, plantation forests and native forests are juxtaposed. The Tasmanian pademelon Thylogale billardierii and the red-necked wallaby Macropus rufogriseus rufogriseus are among the most abundant native pest herbivore species in Tasmania. Aims We aimed to determine the habitat use of pademelons and wallabies in response to (i) environmental and seasonal variation, and (ii) two different wildlife management interventions (shooting and fencing) in an agricultural–forest mosaic in north-eastern Tasmania. Methods Macropod abundance before and after shooting and fencing management interventions were estimated by changes in the rate of deposition of faecal pellets (scats per unit area per time interval) on an array of permanent transects that were stratified across three habitat types (agricultural land, plantation forest, and native forest). An experiment was also conducted to determine the endurance of fresh scats in the three habitats. Key results More than 90% of scats remained undecomposed for over five months, and more than 50% of scats remained undecomposed for over 11 months across the study site. Decomposition rates were significantly influenced by habitat type, specifically, highest in agricultural land and lowest in native forest for both species. Scat deposition rates showed that species abundance was influenced by habitat type and season. Macropod abundance was highest in agricultural land and lowest in native forest. Compared with summer and early autumn, pademelon scat abundance significantly decreased in late autumn and spring on agricultural land but showed no change for plantation forest or native forest. Wallaby scats showed similar seasonal trends for all three habitats, lower in late autumn and spring compared with summer and early autumn. Following each of the management interventions, macropod scat deposition rates decreased predominantly on agricultural land. This effect decreased with increasing distance from intervention loci. Conclusions We demonstrate that scat monitoring provides a useful survey technique for the assessment of macropod habitat use, and show that macropods select for agricultural habitats. Shooting and fencing interventions reduced the use of agricultural habitats, but this effect was localised. Implications A whole-landscape perspective is required when assessing the impacts of management interventions on pest populations. Results highlight the formidable challenges in controlling native herbivores in habitat mosaics, given the localised effects of management interventions and the importance of environmental and seasonal factors as drivers of habitat use.

Bennett’s wallaby ( Macropus rufogriseus) marrow quality vs quantity: evaluating human decision-making and seasonal occupation in late Pleistocene Tasmania

A pilot study on the fatty acid composition of the bone marrow, brain tissue and muscle (flesh) of the medium-sized marsupial the Bennett’s wallaby ( Macropus rufogriseus), aimed to explore the mechanisms behind the dominance of this human prey species in late Pleistocene southwest Tasmania. In particular it was designed to investigate why humans frequently selected and transported the wallaby hindquarters, breaking open their longbones and metatarsals to expose the marrow cavity, when these animals could easily have been transported whole. Initial results indicated that the percentage of unsaturated fatty acids particularly palmitoleic acid (C 16:1) and oleic acid (C 18:1) in the bone marrow increased distally away from the body core temperature or the heart, towards the hands and feet. There is a corresponding decrease in the percentage of the saturated fatty acids, particularly palmitic acid (C 16:0) and stearic acid (C 18:0). This is similar to the pattern identified in placental artiodactyls (even-toed ungulates) and rodents. The preliminary study was then expanded to a year-long seasonal experiment involving wallabies from different Tasmanian environmental and climatic zones to see if this can better help explain the apparent seasonal occupation of caves indicated from recent Bennett’s wallaby teeth annuli (Skeletochronological studies). It was found that the Bennett’s wallaby remained a relatively stable and dependable resource throughout the year, with environmental, altitudinal and seasonal variability having only a minor effect on the fatty acid composition or nutritional quality of their bone marrow, muscle and brain tissue. Interestingly, the animals had increased desirable unsaturated fatty acids in their marrow, muscle and brain during the winter and spring with decreased amounts during the summer and autumn. This lends support to an earlier Kidney Fat Index (KFI) analysis for the Bennett’s wallaby which recorded significant seasonal differences within and between regions, indicating an environmental effect on overall body condition. Fatty acid analysis of the Common wombat Vombatus ursinus, the Tasmanian pademelon Thylogale billardierii, and the Forester or Eastern Grey kangaroo Macropus giganteus were also included to ascertain why these animals remained constant, although minor, prey species throughout the late Pleistocene. While the results from these animals were similar to the Bennett’s wallaby, there were some unexpected and surprising patterns that require further investigation. All of these fatty acid analyses have important implications for understanding human decision-making and occupation of southwest Tasmania during the Last Glacial Maximum, for modern dietary and palaeodietary benefits, and for understanding the physiology of these unique Australian marsupials.

Use of a Sustained Release Chlorhexidine Varnish as Treatment of Oral Necrobacillosis in Macropus spp.

Oral necrobacillosis or lumpy jaw is a common cause of morbidity and mortality affecting captive macropods. This article describes several cases of a new treatment regimen using a sustained release chlorhexidine varnish applied locally to the teeth and the gingivae of two Macropus species, eastern grey kangaroos (Macropus gigantus) from Gan-Garoo Australian Park and a red-necked wallaby (Macropus rufogriseus fruticus) from The Tisch Family Zoological Gardens in Jerusalem. The varnish was applied using a horsehair paint brush as three 1- to 2-mm thick layers. The active ingredient in the varnish was the disinfectant chlorhexidine. Results indicated that use of an intraoral sustained release varnish significantly shortens the treatment time and may prevent recurrence.