Macropus Rufogriseus Research Articles

EVALUATION OF NONINVASIVE OSCILLOMETRIC BLOOD PRESSURE MONITORING IN ANESTHETIZED BENNETT'S WALLABIES (MACROPUS RUFOGRISEUS).

The objective of this study was to determine the accuracy of a noninvasive oscillometric method in relation to invasively measured blood pressure in anesthetized Bennett's wallabies (Macropus rufogriseus) and also to compare the accuracy of two commonly used oscillometric blood pressure monitors (manufactured by Cardell and Datascope). Eleven animals were anesthetized, and each animal was instrumented with an arterial catheter in the right medial metatarsal artery connected to a pressure transducer to obtain invasive measurements of systolic (SAP), diastolic (DAP), and mean (MAP) arterial blood pressure as well as a pressure waveform. A cuff connected to an oscillometric device was placed on the base of the tail for noninvasive measurements. Paired data from noninvasive and invasive blood pressure measurements (SAP, DAP, and MAP) were obtained every 5 min for 60 min. Bland-Altman plots were used to compare invasive and noninvasive measurements and calculate bias and 95% limits of agreement for SAP, DAP, and MAP. For both monitors, the bias of SAP, DAP, and MAP was significant, although the bias of the Cardell was consistently lower than that of the Datascope for all parameters. Limits of agreement were wide for all parameters. In conclusion, when using an oscillometric blood pressure monitor on anesthetized Bennett's wallabies, trends in blood pressure may be monitored, although all displayed readings may not represent the true blood pressure measurement. Indirect measurements of blood pressure made with the oscillometric device cannot substitute for direct measurements.

Activity budget and spatial distribution of Bennett's wallabies (Macropus rufogriseus) in open versus closed exhibit designs.

Although many studies investigating the impacts of zoo exhibit designs on captive animals exist, none have been performed on how they influence the behavior and welfare of captive Bennett's wallabies (Macropus rufogriseus). Here, we assess the impact of exhibit design on the activity budget and spatial distribution of Bennett's wallabies. We compared animal behavior in two open exhibits (i.e. physical interaction between animals and visitors permitted) to two closed exhibits (i.e. physical interaction between animals and visitors prohibited). Behavioral data were collected using focal sampling, and spatial distribution was recorded on exhibit maps at regular time intervals. We found a significant increase in feeding and interactive behaviors in closed exhibits in comparison to open exhibits. However, other behaviors such as resting, locomotion, and vigilance did not vary with design. Functional use of space was similar between both designs; however, the effect of habituation may be relevant to consider in future studies. Although some support for visitor effects were present, our study provided no evidence for strong impacts of exhibit design on Bennett's wallaby welfare. Our study emphasizes the need for additional research into the impacts of how zoo environments affect Bennett's wallaby behavior and welfare.

First report of nymphs of the introduced pentastomid, Linguatula serrata, in red-necked wallabies (Notamacropus rufogriseus) in Australia

The presence of nymphs of the introduced pentastomid parasite Linguatula serrata is verified in a native Australian animal, the red-necked wallaby (Notamacropus rufogriseus), for the first time. Morphological and genetic analyses confirmed the species identification, with a 100% genetic match to samples collected from various introduced animals from the same location in Kosciuszko National Park. Previous reports of pentastomids, including possible Linguatula sp. infections, in native animals are discussed. More research is required to confirm the validity of these reports.

Habitat suitability, live abundance and their link to road mortality of Tasmanian wildlife

ContextTasmania has been called the roadkill capital of Australia. However, little is known about the population-level impact of vehicle mortality on native mammals in the island state. AimsThe aims were to investigate the predictability of roadkill on a given route, based on models of species distribution and live animal abundance for three marsupial species in Tasmania – the Tasmanian pademelon (Thylogale billardierii), Bennett’s wallaby (Macropus rufogriseus) and the bare-nosed wombat (Vombatus ursinus) – and to assess the possibility of predicting the magnitude of state-wide road mortality based on live animal abundance. MethodsRoad mortality of the three species was measured on eight 15-km road segments in south-eastern Tasmania, during 16 weeks over the period 2016–17. Climate suitability was predicted using state-wide geographical location records, using species distribution models, and counts of these species from 190 spotlight survey roads. Key resultsThe Tasmanian pademelons were the most frequently killed animal encountered over the study period. Live abundance, predicted by fitting models to spotlight counts, did not correlate with this fatality rate for any species. However, the climate suitability index generated by the species distribution models was strongly predictive for wombat roadkill, and moderately so for pademelons. ConclusionsAlthough distributional and wildlife abundance records are commonly available and well described by models based on climate, vegetation and land-use predictors, this approach to climate suitability modelling has limited predictability for roadkill counts on specific routes. ImplicationsRoad-specific factors, such as characteristics of the road infrastructure, nearby habitats and behavioural traits, seem to be required to explain roadkill frequency. Determining their relative importance will require spatial analysis of roadkill locations.

Diagnostic Approach and Surgical Management of a Maxillary Molar Tooth Root Infection in a Red-Necked Wallaby (Macropus rufogriseus) Carrying a Joey

Diagnostic Approach and Surgical Management of a Maxillary Molar Tooth Root Infection in a Red-Necked Wallaby (Macropus rufogriseus) Carrying a Joey

Current and predicted future distributions of wallabies in mainland New Zealand

ABSTRACTBennett's (Notamacropus rufogriseus) and dama (N. eugenii) wallabies have been increasing in numbers and distribution in mainland New Zealand. Here, we update their current distributions; estimate current rates of spread to predict their future distributions; and describe the extent of suitable habitat for each species. Current distributions based on breeding populations and probable distributions based on outlier confirmed sightings resulting from natural dispersal and illegal liberations suggest that Bennett's and dama wallabies currently occupy between 5322–15,229 km2 (532,200–1,522,900 ha) and 1865–4126 km2 (186,500–412,600 ha), respectively. In 50 years, best- and worst-case estimates predict that they could occupy as much as 5883–44,226 km2 (588,300–4,422,600 ha) and 1912–40,579 km2 (191,200–4,057,900 ha), respectively. Habitat suitability was predicted to be high in the North and South islands, except for areas of high elevation, intensive agriculture with limited woody vegetative cover and large urban centres. In the absence of widespread intensive control, the ranges currently occupied by Bennett's and dama wallabies are predicted to increase by up to 7- and 20-fold (respectively) in 50 years. As their distributions continue to expand, they will become more difficult to control and their impacts more widespread. We suggest that progressively containing wallabies within increasingly smaller distributions and reducing their numbers to minimise damage within those distributions should be considered the top management priorities. If progressive containment is feasible it could lead to eventual eradication.

The distribution and trophic ecology of an introduced, insular population of red-necked wallabies (Notamacropus rufogriseus)

Introduced non-native mammals can have negative impacts on native biota and it is important that their ecologies are quantified so that potential impacts can be understood. Red-necked wallabies (Notamacropus rufogriseus (Desmarest, 1817)) became established on the Isle of Man (IOM), an island with UNESCO Biosphere status, following their escape from zoological collections in the mid-1900s. We estimated wallaby circadial activity and population densities using camera trap surveys and random encounter models. Their range in the IOM was derived from public sightings sourced via social media. Wallaby diet and niche breadth were quantified via microscopic examination of faecal material and compared with those of the European hare (Lepus europaeus Pallas, 1778). The mean (±SE) population density was 26.4 ± 6.9 wallabies/km2, the mean (±SE) population size was 1742 ± 455 individuals, and the species’ range was 282 km2, comprising 49% of the island. Wallaby diets were dominated by grasses, sedges, and rushes; niche breadth of wallabies and hares (0.55 and 0.59, respectively) and overlap (0.60) suggest some potential for interspecific competition and (or) synergistic impacts on rare or vulnerable plant species. The IOM wallaby population is understudied and additional research is required to further describe population parameters, potential impacts on species of conservation interest, and direct and indirect economic costs and benefits.

Interspecific and intraspecific relationships between body mass and diet quality in a macropodid community

The Jarman-Bell principle seeks to explain why smaller herbivore species tend to select higher-quality forage (high protein and high fiber digestibility) than larger herbivore species. This principle may also provide insight into intraspecific differences in resource use in species with pronounced sexual size dimorphism. We examined the relationship between body mass and diet quality in a sexually dimorphic macropodid community comprising larger (17-55 kg) eastern and western gray kangaroos, Macropus giganteus and M. fuliginosus, and smaller (7-23 kg) red-necked and swamp wallabies, M. rufogriseus and Wallabia bicolor. We determined diet composition through microhistological analysis of fecal pellets, and then assessed diet quality by measuring available nitrogen and fiber (cellulose and hemicellulose) in the plants consumed. We predicted that the smaller species and smaller individuals within species would consume the highest-quality diets. The concentration of fiber consumed differed significantly among species; available nitrogen concentration did not. As predicted, the lowest-fiber diet was consumed by a wallaby (small species) and the highest-fiber diet was consumed by a kangaroo (large species). Contrary to predictions, we detected no sex-based differences in diet quality. However, large male red-necked wallabies consumed diets containing harder-to-digest fiber than small male red-necked wallabies. Body mass and diet type were related between and within species. However, body mass did not account for all differences in diet quality between species; swamp wallabies consumed less fiber than similarly sized red-necked wallabies. These differences are likely related to other adaptations that allow swamp wallabies to better tolerate tannin-rich browse-dominated diets.

Maxillary nerve block as part of a multimodal anaesthesia in a lactating red-necked wallaby (Macropus rufogriseus) undergoing partial maxillectomy

A partial maxillectomy was performed in a four-year-old, 12-kg lactating female red-necked wallaby or Bennett’s wallaby (Macropus rufogriseus) with a diagnosis of oral necrobacillosis or ‘lumpy jaw disease’. After intramuscular...

An adaptive management case study for managing macropods on Maria Island National Park, Tasmania, Australia: adding devils to the detail

Adaptive management is driven by structured decision making and evidence from monitoring in a ‘learning’ framework that guides management actions. In a conservation context, this iterative approach includes evaluation of the impacts on natural processes. On Maria Island National Park, Tasmania, Australia, introduced Forester kangaroo, Bennetts wallaby and Tasmanian pademelon have been intensively managed by an annual cull since 1994. Management actions were triggered by high parasite loads, intense grazing pressure and high juvenile mortality during drought periods. Criticism of the annual cull from animal welfare groups initiated the development of an adaptive management approach for decision making that replaces the historic ‘trial and error’ process. Following a comprehensive review of the existing macropod management program in 2011, an integrated monitoring strategy was established to provide evidence for informed decision making. Assessments of animal health and estimates of population trends are the key indicators for management actions to occur. Maintaining viable macropod populations and protecting natural values form the basis of management objectives. Management actions in each year, for each species, represent ‘treatments’ as spatial replication is not possible at such a small scale. An adaptive management approach for macropod management on Maria Island has resulted in only one species being culled in 2014 and 2015 for the first time in almost 20 years. However the recent introduction of a major predator, the Tasmanian devil, has increased uncertainty for long-term macropod management on Maria Island with no cull occurring in 2016 and 2017.

Dietary partitioning of Australia's two marsupial hypercarnivores, the Tasmanian devil and the spotted-tailed quoll, across their shared distributional range.

Australia’s native marsupial fauna has just two primarily flesh-eating ‘hypercarnivores’, the Tasmanian devil (Sarcophilus harrisii) and the spotted-tailed quoll (Dasyurus maculatus) which coexist only on the island of Tasmania. Devil populations are currently declining due to a fatal transmissible cancer. Our aim was to analyse the diet of both species across their range in Tasmania, as a basis for understanding how devil decline might affect the abundance and distribution of quolls through release from competition. We used faecal analysis to describe diets of one or both species at 13 sites across Tasmania. We compared diet composition and breadth between the two species, and tested for geographic patterns in diets related to rainfall and devil population decline. Dietary items were classified into 6 broad categories: large mammals (≥ 7.0kg), medium-sized mammals (0.5–6.9kg), small mammals (< 0.5kg), birds, reptiles and invertebrates. Diet overlap based on prey-size category was high. Quoll diets were broader than devils at all but one site. Devils consumed more large and medium-sized mammals and quolls more small mammals, reptiles and invertebrates. Medium-sized mammals (mainly Tasmanian pademelon Thylogale billardierii), followed by large mammals (mainly Bennett’s wallaby Macropus rufogriseus) and birds, were the most important prey groups for both species. Diet composition varied across sites, suggesting that both species are flexible and opportunistic foragers, but was not related to rainfall for devils. Quolls included more large mammals but fewer small mammals and invertebrates in their diet in the eastern drier parts of Tasmania where devils have declined. This suggests that a competitive release of quolls may have occurred and the substantial decline of devils has provided more food in the large-mammal category for quolls, perhaps as increased scavenging opportunities. The high diet overlap suggests that if resources become limited in areas of high devil density, interspecific competition could occur.

Composition of marsupial zona pellucida: a molecular and phylogenetic approach.

The zona pellucida (ZP) is an extracellular matrix that surrounds mammalian oocytes. In eutherians it is formed from three or four proteins (ZP1, ZP2, ZP3, ZP4). In the few marsupials that have been studied, however, only three of these have been characterised (ZP2, ZP3, ZP4). Nevertheless, the composition in marsupials may be more complex, since a duplication of the ZP3 gene was recently described in one species. The aim of this work was to elucidate the ZP composition in marsupials and relate it to the evolution of the ZP gene family. For that, an in silico and molecular analysis was undertaken, focusing on two South American species (gray short-tailed opossum and common opossum) and five Australian species (brushtail possum, koala, Bennett's wallaby, Tammar wallaby and Tasmanian devil). This analysis identified the presence of ZP1 mRNA and mRNA from two or three paralogues of ZP3 in marsupials. Furthermore, evidence for ZP1 and ZP4 pseudogenes in the South American subfamily Didelphinae and for ZP3 pseudogenes in two marsupials is provided. In conclusion, two different composition models are proposed for marsupials: a model with four proteins (ZP1, ZP2 and ZP3 (two copies)) for the South American species and a model with six proteins (ZP1, ZP2, ZP3 (three copies) and ZP4) for the Australasian species.

Speciation Generates Mosaic Genomes in Kangaroos.

The iconic Australasian kangaroos and wallabies represent a successful marsupial radiation. However, the evolutionary relationship within the two genera, Macropus and Wallabia, is controversial: mitochondrial and nuclear genes, and morphological data have produced conflicting scenarios regarding the phylogenetic relationships, which in turn impact the classification and taxonomy. We sequenced and analyzed the genomes of 11 kangaroos to investigate the evolutionary cause of the observed phylogenetic conflict. A multilocus coalescent analysis using ∼14,900 genome fragments, each 10 kb long, significantly resolved the species relationships between and among the sister-genera Macropus and Wallabia. The phylogenomic approach reconstructed the swamp wallaby (Wallabia) as nested inside Macropus, making this genus paraphyletic. However, the phylogenomic analyses indicate multiple conflicting phylogenetic signals in the swamp wallaby genome. This is interpreted as at least one introgression event between the ancestor of the genus Wallabia and a now extinct ghost lineage outside the genus Macropus. Additional phylogenetic signals must therefore be caused by incomplete lineage sorting and/or introgression, but available statistical methods cannot convincingly disentangle the two processes. In addition, the relationships inside the Macropus subgenus M. (Notamacropus) represent a hard polytomy. Thus, the relationships between tammar, red-necked, agile, and parma wallabies remain unresolvable even with whole-genome data. Even if most methods resolve bifurcating trees from genomic data, hard polytomies, incomplete lineage sorting, and introgression complicate the interpretation of the phylogeny and thus taxonomy.

Treatment of mandibular osteomyelitis in two red-necked wallabies (Macropus rufogriseus) by means of intensive long-term parenteral drug administration and serial computed tomographic monitoring

CASE DESCRIPTION 2 female red-necked wallabies (Macropus rufogriseus) were evaluated because of sudden-onset mandibular swelling, ptyalism, and hyporexia. CLINICAL FINDINGS Physical examination revealed a mandibular swelling with a fluctuant center in both wallabies. Hematologic analysis revealed leukocytosis with a mature neutrophilia and monocytosis in one wallaby (case 1) and a slight neutrophilia, hyperglobulinemia, and high serum alanine aminotransferase activity in the other (case 2). Cytologic examination of the swelling revealed a uniform population of gram-negative rods in case 1 and neutrophilic inflammation in case 2. Radiography revealed a soft tissue mandibular swelling with osteolucency around mandibular incisor roots in both wallabies. Computed tomography revealed changes consistent with chronic active mandibular osteomyelitis and reactive bone formation, but also sequestra formation not appreciable via radiography. TREATMENT AND OUTCOME Long-term antimicrobial treatment was initiated with clindamycin (17 to 21 mg/kg [7.7 to 9.5 mg/lb], IV, q 12 h for 40 to 55 days) and high-dose benzathine penicillin G (80,000 U/kg [36,364 U/lb], SC, q 12 h for 150 days). Serial CT was performed to evaluate response to treatment and resolution of disease. A CT scan 18 months after the initial evaluation revealed complete resolution of osteomyelitis and sequestra. CLINICAL RELEVANCE Advanced imaging and long-term treatment and management were integral to the successful outcome for these wallabies, given that the osseous changes visible on CT images were not visible on standard radiographs, guiding therapeutic decision-making. This report provides new therapeutic and diagnostic monitoring information to assist clinicians with similar cases.

NEWLY DESCRIBED TOXOPLASMA GONDII STRAIN CAUSES HIGH MORTALITY IN RED NECKED WALLABIES (MACROPUS RUFOGRISEUS) IN A ZOO.

This manuscript describes an outbreak of fatal toxoplasmosis in wallabies. Ten adult red necked wallabies (Macropus rufogriseus) were imported from New Zealand to the Virginia Zoo. Agglutination testing upon admission into quarantine showed all animals to be negative for antibodies to Toxoplasma gondii. Nine of these wallabies died from acute toxoplasmosis within 59-565 (average 224) days after being moved onto exhibit. Clinical signs included lethargy, diarrhea, tachypnea, and ataxia that progressed rapidly; death without premonitory signs occurred in one case. Histopathologic examination revealed interstitial pneumonia, encephalomyelitis, myositis, enteritis, and myocarditis. The diagnosis was confirmed through serologic, histopathologic, and polymerase chain reaction (PCR) testing. Multilocus PCR-RFLP (restriction fragment length polymorphism) genotyping revealed that the first six animals were infected by a previously undiscovered Toxoplasma gondii genotype, designated as ToxoDB PCR-RFLP genotype No. 263. These six cases survived for an average of 118 days on exhibit before succumbing to toxoplasmosis. The other three wallabies were infected with a Toxoplasma gondii strain of ToxoDB PCR-RFLP genotype No. 4, which is a common strain type circulating in wild animals in North America. These three cases survived for an average of 435 days on exhibit before succumbing to toxoplasmosis. The outbreaks of toxoplasmosis in these wallabies are likely from two different sources. Furthermore, the results highlight Toxoplasma gondii PCR-RFLP genotyping in parasite diagnosis and understanding parasite transmission and potential mitigation procedures.

Early expression of manual lateralization in bipedal marsupials.

Robust lateralization in forelimb use has recently been found in bipedal, but not quadrupedal, marsupial mammals. The link between bipedality and handedness, occurring in both marsupials and primates, remains to be investigated. To shed light on the developmental origins of marsupial manual lateralization, infants of macropod marsupials were examined before and shortly after the acquisition of habitual bipedal posture and locomotion. Forelimb preferences were assessed in natural, not artificially evoked, behaviors of infant red-necked wallaby in the wild and infant eastern gray kangaroo in free-ranging captivity. Pouch young of both species showed population-level left-forelimb preference when manipulating food objects, such as leaves and grass blades. This result provides the first report of lateralization in pouch young marsupials and rare evidence of lateralized manual activity in early mammalian ontogenesis. Young-at-foot juveniles of eastern gray kangaroo preferred to use the left forelimb to manipulate the mother's pouch edge as previously shown for red-necked wallaby. In both species, the direction of biases in manipulative behavior for young-at-foot and pouch young was the same as in adults. Forelimb preferences in offspring were positively correlated with the forelimb preferences of their mothers. Our results strongly suggest that the emergence of individual and population-level forelimb preferences in macropod infants precedes the onset of independent standing and locomotion. In all probability, manual lateralization in bipedal marsupials, such as kangaroos and wallabies, is not determined by the acquisition of habitual bipedality in the course of ontogenesis. (PsycINFO Database Record

Pharmacokinetics of intravenous clindamycin phosphate in captive Bennett's wallabies (Macropus rufogriseus).

This study was designed to investigate the pharmacokinetics of clindamycin, a lincosamide antibiotic, in Bennett's wallabies. The pharmacokinetic properties of a single intravenous (IV) dose of clindamycin were determined in six wallabies. A single 20-min IV infusion of 20mg/kg of clindamycin was administered, followed by blood collection prior to, and up to 12hr after clindamycin administration. Plasma clindamycin concentrations were determined by high-pressure liquid chromatography (HPLC) with ultraviolet (UV) detection. Pharmacokinetic variables were calculated using a two-compartment model with first order elimination which best fit the data. The mean volume of distribution at steady-state, distribution half-life, and elimination half-life were 898.25ml/kg, 0.16hr, 1.79hr, respectively. No adverse effects were noted after IV administration.

Oesophageal and stomach nematode communities in three sympatric macropodid species in coastal and montane environments in southeastern New South Wales

ABSTRACTThe parasitic nematode communities are described from the oesophagi and stomachs of eastern grey kangaroos, Macropus giganteus (Shaw), red-necked wallabies, Notamacropus rufogriseus (Desmarest) and swamp wallabies, Wallabia bicolor (Desmarest) (Marsupialia: Macropodidae) from coastal and montane habitats in southeastern New South Wales. The number of nematode species recorded for each macropodid species was similar to that recorded in previous studies in Victoria. Overall diversity of nematode communities was greatest in N. rufogriseus and greater in montane than in coastal localities in both N. rufogriseus and M. giganteus. Community similarity between coastal and montane localities was highest in N. rufogriseus compared to M. giganteus and W. bicolor. Few nematode species were shared between host species. There were more significant differences in species prevalences than intensities of infection in M. giganteus between coastal and montane localities, less so for N. rufogriseus but these were not calculated for W. bicolor due to small montane sample size. Community differences between macropodid species were attributable primarily to host-specific parasite species. This is the first comparison of helminth communities in macropodids in a replicated study in different habitats at similar latitudes.

Adaptations of large marsupials to survival in winter snow cover: locomotion and foraging

The small extent of seasonally snow-covered Australian mountains means that there has not been a great selective pressure on the mammalian fauna for adaptations to this environment. Only one large marsupial, the common wombat (Vombatus ursinus (Shaw, 1800)), is widespread above the winter snowline. In the past 20 years, with snow depth and duration declining, the swamp wallaby (Wallabia bicolor (Desmarest, 1804)) has become more common above the winter snowline. The red-necked wallaby (Macropus rufogriseus (Desmarest, 1817)) is common in alpine Tasmania where seasonal snow cover is neither as deep nor as long-lasting as on the mainland, but has only been recorded regularly above the winter snowline in the mainland Snowy Mountains since 2011. This study examines morphological aspects of locomotion of these three herbivorous marsupials in snow. The wombat is the best adapted to snow, with quadrupedal gait and an expanded home range allowing it to locate and feed on the same plant groups as it does at lower elevation. Wallabies are poorly adapted to locomotion in snow, but the browsing swamp wallaby is able to maintain its dietary habit by feeding on exposed shrubs in deep snow, whereas the red-necked wallaby, which depends more on grazing, appears constrained to areas where snow is shallow.

Prothrombin time and activated partial thromboplastin time using a point-of-care analyser (Abaxis VSpro®) in Bennett's wallabies (Macropus rufogriseus).

There are few reports of coagulation times in marsupial species. Blood samples collected from 14 Bennett's wallabies (Macropus rufogriseus) under anaesthesia during routine health assessments were analysed for prothrombin time (PT) and activated partial thromboplastin time (aPTT) using a point-of-care analyser (POC) (Abaxis VSPro®). The wallabies had an aPTT mean of 78.09 s and median of 78.1 s. The PT for all wallabies was greater than 35 s, exceeding the longest time measured on the POC. Although PT was significantly longer, aPTT was similar to the manufacturer's domestic canine reference range.