Czech family policies have gone through dramatic changes since the 1989 transition to a market economy, resulting into the highest employment gap between women with and without pre-school children in OECD. This paper focuses on the 1995 Czech Parental Benefit reform which extended the payment of universal parental benefits to 4 years instead of 3 without an equivalent extension of job-protected parental leave, leaving to mothers the choice of either guaranteed return to employment or an additional 12 months of benefits. The study relies on a difference-in-differences strategy to assess the net effect of this large-scale reform on mothers’ labour market participation. I find a strong negative impact on mothers’ probability of return to work at the end of parental leave, with a heterogeneous size with respect to their educational attainment. I also find evidence of the persistence of this detrimental effect on mothers’ employment beyond the short-term horizon targeted by the legislators.