Benefits Of Probiotics Research Articles

Technological Trends Involving Probiotics in the Treatment of Diabetic Neuropathy: A Patent Review (2009-2022).

Diabetic neuropathy (DN) causes neuropathic pain, and current treatments are unsatisfactory. Recently studies have demonstrated an assertive correlation between gut microbiota and pain modulation. Considering the emerging search for new therapies for the control of DN and the growing commercial interest in the probiotics market, this study aimed to provide patents on the use of probiotics in the control of DN. This is a patent prospection performed in the Espacenet Patent database, using the association of keywords and IPC related to probiotics in medical preparations and foods, from 2009 to December 2022. Results have shown that in 2020, there was a boom in patent filing in the area. Asian countries accounted for more than 50% of all 48 inventions (n = 48), with Japan as the only applicant in 2021. Products being developed in recent years point to effects that may represent an advancement in DN treatment, such as reduced concentration of pro-inflammatory mediators, metabolites and neurotransmitters release, and hypoglycemic potential. All effects were more related to the Lactobacillus and Bifidobacterium genera, associated with more than one property mentioned. The mechanisms attributed to the microorganisms suggest the therapeutic potential of probiotics in the non-pharmacological treatment of pain. New applications for probiotics have resulted from great research interest by academia, but also reflect commercial interests despite the paucity of clinical trials. Thus, the present work supports the evolution of research to explore the benefits of probiotics and their clinical use in DN.

Fermentation in aquafeed processing: Achieving sustainability in feeds for global aquaculture production

AbstractImproving feed suitability and bolstering the global upsurge in fish production is a strategic challenge for aquaculture. The utilization of plant and animal derived ingredients in aquafeed for achieving sustainable in finfish aquaculture is affected by various factors, including the presence of antinutritional factors, lowered nutrient bioavailability, indigestible particles and microbial contaminants. Applying fermentation to overcome these problems in aquafeed ingredients has received considerable attention in recent years as fermentation provides health‐promoting probiotic benefits to host organisms. Fermentation has also been shown in many studies to improve nutrient availability and bioavailability of feed, increased palatability and digestibility and eliminate anti‐nutritional compounds in dietary feed ingredients, making them more easily digestible which eventually improve growth and health performance of fish. Therefore, it is imperative to accelerate the use of fermented feedstuffs as aquafeed if sustainable aquaculture is to be achieved. This review reported the various methods of fermentation, characteristics of fermented feed ingredients, factors that are considered during fermentation and overall nutritional quality of fermented feed ingredients for aquaculture production. The role of fermented feed ingredients for various farmed species in terms of growth, feed utilization, gut microbiota composition, immunity and disease resistance in fish is thoroughly discussed. The possible drawbacks associated with the fermentation process are also discussed in the article.

Interpretation of vaginal metagenomic characteristics in different types of vaginitis.

Vaginitis is one of the most common gynecological diseases, mostly caused by infections of pathogens such as Candida albicans and Gardnerella vaginalis. In recent years, it has been found that the stability of the vaginal flora plays an important role in vaginitis. Furthermore, the abundant Lactobacillus-producing rich lactic acid in the vagina provides a healthy acidic environment such as Lactobacillus crispatus. The metabolites of Lactobacillus can inhibit the colonization of pathogens. Here, we collected the vaginal samples of patients with bacterial vaginitis (BV), vulvovaginal candidiasis (VVC), and BV combined with VVC to discover the differences and relationships among the different kinds of vaginitis by metagenomic sequencing. Furthermore, because of the importance of L. crispatus in promoting vaginal health, we isolated multiple strains from vaginal samples of healthy females and chose the most promising strain with potential probiotic benefits to provide clinical implications for treatment strategies.

Screening and identification of probiotics from the intestinal tract of largemouth bass (Micropterus salmoides) for use as a feed additive and bacterial infection control

Probiotics regulate the health of the host by influencing digestion and absorption, intestinal morphology, microbial composition, and the host's defense against infection. The genus Bacillus encompasses several known probiotics capable of surviving harsh feed processing and extended storage conditions. More importantly, these microbes can still confer probiotic benefits even when administered to animals in the form of spores. Therefore, the screening of Bacillus strains with probiotic potential holds significant importance for aquaculture. In our study, we screened a total of 75 Bacillus strains, leading to the identification of a promising candidate strain, Bacillus velezensis (BSG-2), which has the excellent performance of antagonism towards pathogenic bacteria including Aeromonas hydrophila, Edwardsiella piscicida, and Nocardia seriolae. Genome sequencing provided the evidence of the antibacterial-related genes and gene clusters associated with secondary metabolism. In addition, BSG-2 has a prominent ability to endure harsh environment and secret digestive enzymes, which indicated that BSG-2 has the potential to survive and promote digestion in the intestinal tract through the gastrointestinal tract. Importantly, feeding 56 days, BSG-2 increased activities of digestive and immune-related enzymes, and then promoted growth and decreased mortality and tissue damage of largemouth bass after Nocardia seriolae infection. Collectively, our findings demonstrate that Bacillus velezensis BSG-2 is a promising probiotic candidate offering multiple benefits for largemouth bass.

A systematic assessment on the influence of probiotics on patients with Inflammatory Bowel Disorder

Inflammatory bowel disease (IBD) causes persistent mucosal inflammation, and mounting evidence points to the intestinal microbiota's critical involvement in this process. New molecular techniques have also helped to identify dysbiosis in these individuals. Many potential probiotic product action mechanisms counteract IBD etiological causes. The study aimed to explain the benefits of probiotics for IBD and thoroughly evaluate current trials using probiotics to treat IBD in grownups. A specific search was done using specified keywords on October 1, 2011, using the supplied date range. Only English-language, full-text articles on clinical results for adult patients were addressed. Based on disease type and disease activity, the 41 eligible studies were classified. Pooled odds ratios were calculated only when there were many available randomized controlled trials per probiotic for a particular patient group. According to well-designed randomized controlled studies, few probiotics are effective in treating IBD at this time. It was only feasible to conduct meta-analyses on a limited number of studies, which yielded an overall risk ratio of 2.70 for inducing remission in active U.C. with Bifido fermented milk versus placebo or no adjuvant treatment. Very Specific Lactic Acid Bacteria (VSL#3) enables people to go into remission active U.C. compared to placebo. To support the favorable outcomes for inactive ulcerative colitis (U.C.), it is still a need to conduct better-conducted investigations in regard to the variety of drug VSL#3 in patients with both active and dormant U.C. U.C. based on intention-to-treat analyses by many independent research organizations. According to the existing data, probiotic usage in Crohn’s Disease (CD) has yet to be shown. The location and subtypes of certain diseases should be the focus of future research. Selecting probiotic strains for certain disease entities and illness sites will be easier with more knowledge of the mechanisms of probiotic strains and the etiology of IBD.

The Impact of Oral Probiotics on Laboratory Parameters in Patients With Alcohol Toxicity: A Single-blinded Intervention Study

Background: Alcohol toxicity is a significant medical emergency with implications for patient management and outcomes. This single-blinded randomized intervention study investigated the effects of oral probiotics on various laboratory parameters in patients with acute alcohol toxicity. Methods: A total of 30 eligible patients were randomly assigned to either the control (placebo) or intervention (oral probiotics) group. Results: While the study did not reveal a significant impact on the length of hospital stay (LOHS), it did demonstrate notable improvements in laboratory variables, including pH, serum glutathione level, serum vitamin B6 level, and O2 saturation, in the probiotic group. Conclusion: These findings suggest the potential benefits of probiotics in mitigating certain aspects of alcohol toxicity.

Dietary supplementation with probiotics promotes weight loss by reshaping the gut microbiome and energy metabolism in obese dogs.

Obesity and overweight among companion animals are significant concerns, paralleling the issues observed in human populations. Recent research has highlighted the potential benefits of various probiotics in addressing weight-related changes, obesity, and associated pathologies. In this study, we delved into the beneficial probiotic mechanisms in high-fat-induced obese canines, revealing that Enterococcus faecium IDCC 2102 (IDCC 2102) and Bifidobacterium lactis IDCC 4301 (IDCC 4301) have the capacity to mitigate the increase in body weight and lipid accumulation in obese canines subjected to a high-fat diet and hyperlipidemic Caenorhabditis elegans (C. elegans) strain VS29. Both IDCC 2102 and IDCC 4301 demonstrated the ability to reduce systemic inflammation and hormonal disruptions induced by obesity. Notably, these probiotics induced modifications in the microbiota by promoting lactic acid bacteria, including Lactobacillaceae, Ruminococcaceae, and S24-7, with concomitant activation of pyruvate metabolism. IDCC 4301, through the generation of bacterial short-chain fatty acids and carboxylic acids, facilitated glycolysis and contributed to ATP synthesis. Meanwhile, IDCC 2102 produced bacterial metabolites such as acetic acid and butyric acid, exhibiting a particular ability to stimulate dopamine synthesis in a canine model. This stimulation led to the restoration of eating behavior and improvements in glucose and insulin tolerance. In summary, we propose novel probiotics for the treatment of obese animals based on the modifications induced by IDCC 2102 and IDCC 4301. These probiotics enhanced systemic energy utilization in response to high caloric intake, thereby preventing lipid accumulation and restoring stability to the fecal microbiota. Consequently, this intervention resulted in a reduction in systemic inflammation caused by the high-fat diet.IMPORTANCEProbiotic supplementation affected commensal bacterial proliferation, and administering probiotics increased glycolysis and activated pyruvate metabolism in the body, which is related to propanate metabolism as a result of pyruvate metabolism activation boosting bacterial fatty acid production via dopamine and carboxylic acid specialized pathways, hence contributing to increased ATP synthesis and energy metabolism activity.

Scrutinizing Local Probiotic Supplements and Drinks Available in the Bangladesh Market

The host's health may benefit from the probiotic microorganisms found in many products available on the market. In addition to food, these products include dietary supplements, food for special medical purposes, medicines, cosmetics, and medical devices. These products have anywhere from one to a dozen strains of bacteria from the same or different species, and sometimes they also have strains of fungi. Since the health benefits of probiotics depend on the strain, the number of cells in a dose, and the absence of pathogenic microorganisms, it is crucial to regulate the quality of probiotics. Depending on how a product is classified, how it looks, and how many microorganisms it has, it is very important to count and identify the microorganisms correctly. We investigated nine probiotic products in this study: five commercially available probiotic supplements and four probiotic drinks. The majority of commercial products did not contain all of the labeled lactic acid bacteria, nor did they contain any possibly harmful microorganisms. To ensure that consumers in Bangladesh obtain good products, probiotic-containing product rules and regulations should be developed. Furthermore, it should be the duty of probiotics-containing product manufacturers to provide consumers with information that is accurate, reliable, and compliant with legal and scientific requirements.

Preparation and characterisation of novel casein-gum Arabic composite microcapsules for targeted in vivo delivery of Lactiplantibacillus plantarum A3.

The health benefits of probiotics in the body are predicated on their ability to remain viable in harsh gastrointestinal conditions and complex pathological microenvironments. Casein and gum Arabic (GA), with dual emulsifying and stabilising effects in colloidal systems. Therefore, the objective of this research was to develop a novel microcapsule to encapsulate Lactiplantibacillus plantarum A3 using casein and GA as wall materials to improve the survival of the bacteria during gastrointestinal digestion, storage and lyophilization. The casein and GA composite microcapsules were prepared and characterised by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM). It was found that the microcapsules had stable morphology, uniform size and spherical shape. The results revealed that the encapsulation of microcapsules significantly improved the survival of L. plantarum A3 in gastrointestinal fluid environment (5.52× 109 cfu/ml) and lyophilization treatment (6.25× 109 cfu/ml). Furthermore, the microencapsulated L. plantarum A3 exhibited an improved ability to regulate intestinal microbiota by effectively increasing the relative abundance of Bacteroidetes, Proteobacteria and Actinobacteria and decreasing the relative abundance of Firmicutes in vivo. The findings of the study will help to design a lactic acid bacteria encapsulation system based on the gastrointestinal environment and provide a basis for the development of probiotic functional products.

Assessment of Knowledge, Perception, and Practices Regarding Probiotics and Prebiotics Among Clinicians in Saudi Arabia: A Pilot Study.

Probiotics and prebiotics are important in preventing different diseases such as irritable bowel syndrome (IBS), which is a functional bowel disorder characterized by changes in bowel habits. Considering the limited studies on this topic, the present study was conducted to assess both gastroenterologist (GI) physicians' and dietitians' practices in recommending probiotics and prebiotics for IBS patients, as well as to measure their professional educational history on probiotics and prebiotics. A descriptive cross-sectional study was performed to investigate the knowledge, use, and perceptions of probiotics and prebiotics of GI physicians and dietitians at governmental hospitals in Jeddah, Saudi Arabia. A validated questionnaire was sent to 42 participants of both genders selected randomly, who were all qualified healthcare practitioners. The majority of GI physicians demonstrated high levels of knowledge about the health benefits of probiotics and prebiotics (76.2%). In contrast, most dietitians had lower levels of knowledge (52.4%), representing a significant difference between both groups (p<0.01). 83.3% of GI physicians believed that the use of probiotics and prebiotics was required for IBS patients, as compared with 50.0% of dietitians. In conclusion, GI physicians were shown to be more knowledgeable and believed more in the benefits of using probiotics and prebiotics in IBS patients than dietitians, but most participants were not aware of the probiotic products that are available in pharmacies in Saudi Arabia.

Sugarcane Fermentation: Probiotics and Gut Benefits

Sugarcane Fermentation: Probiotics and Gut Benefits

Co-administration of the prebiotic 1-kestose and the paraprobiotic Lactiplantibacillus plantarum FM8 in magellanic penguins promotes the activity of intestinal Lactobacillaceae and reduces the plc gene levels encoding Clostridium perfringens toxin.

Despite the well-known potential health benefits of prebiotics and non-viable probiotics (paraprobiotics) in various animal species, research regarding their use in penguins is scarce. Our study aimed to investigate the impact of a combined administration of prebiotics and paraprobiotics (referred to here as "parasynbiotics") on the gut microbiome and overall health of Magellanic penguins (Spheniscus magellanicus). The parasynbiotics consisted of 1-kestose, which is a fructooligosaccharide comprising sucrose and fructose, and heat-killed Lactiplantibacillus plantarum FM8, isolated from pickled vegetables. It was administered to eight penguins aged <3 years (Young-group) and nine penguins aged >17 years (Adult-group) for 8 weeks. Results from 16S rRNA sequencing revealed that compared to baseline, parasynbiotic administration significantly decreased the relative abundance of intestinal Clostridiaceae_222000 in both groups and significantly increased that of Lactobacillaceae in the Young-group. Quantitative real-time polymerase chain reaction revealed a significant decrease in the plc gene levels encoding alpha-toxin of Clostridium perfringens in the Young-group after parasynbiotic administration (P=0.0078). In the Young-group, parasynbiotic administration significantly increased the plasma levels of total alpha-globulin (P=0.0234), which is associated with inflammatory responses. Furthermore, exposure of dendritic cells to heat-killed L. plantarum FM8 promoted the secretion of interleukin 10, a major anti-inflammatory cytokine. Overall, parasynbiotic administration enhanced the activity of gut Lactobacillaceae, decreased the levels of C. perfringens and its toxin encoding plc gene, and reduced inflammatory response in penguins. These results provide novel insights into the potential benefits of parasynbiotics for improving penguin health.

Влияние пробиотиков на молочную продуктивность и технологические свойства молока коз зааненской породы

The article presents the results of experimental studies to study the effect of feeding probiotic feed additives Kluver Pro and BeneFIT Basic on milk productivity, composition and technological properties of milk from Saanen goats. It has been established that feeding probiotics Kluver Pro and BeneFIT Basic as part of the diets of lactating goats of the Saanen breed helps to increase milk yield by 9,5‑4,4% (p≤0,001), the mass fraction of fat in milk - by 0,73% (p≤0,05) and 0,11%, mass fraction of protein in milk - by 0,25% (p≤0,05) and 0,19%, as well as milk clotting time - by 7,3% and 5,4% (p≤0,001) and the formation of a denser rennet curd, which is a desirable property in the production of fermented milk products and cheese making

Probiotics in the Treatment of Inflammatory Bowel Diseases.

Inflammatory bowel diseases (IBD) are chronic, incurable inflammatory condition of the gut. They comprise Crohn's disease and ulcerative colitis. Crohn's disease (CD) may affect any tract of the gastrointestinal (GI) tract and is a transmural inflammatory condition; ulcerative colitis (UC), on the other hand, is limited to the mucosal layer of the rectum and colon. Treatment options available for both IBD are notoriously loaded with potentially serious side effects and risks. Although the pathogenesis of IBD involves a complex interaction between genetic, environmental, microbial and immunological factors, there is evidence that the interplay between the microbiota and the GI mucosa has a preponderant role. It is therefore no surprise that in recent years, a growing interest for effective and safer alternatives has focused on the potential role of prebiotics and-especially-probiotics.The mechanisms of action underlying the potential benefits of probiotics in IBD have been largely and quite extensively investigated in vitro and in vivo experiments. In terms of clinical evidence, the results of trials in the induction of remission of active CD or the maintenance of its remission with probiotics have been so far largely disappointing, to the point that their use in this disease cannot be at present recommended.On the contrary, for the treatment as well as for maintenance therapy of UC, there is clinical evidence of efficacy for some specific strains or multi-strain preparations.It is evident that this is a rapidly evolving and promising field; more data are very likely to yield a better understanding on what strains and in what doses should be used in different specific clinical settings, as we expect new and exciting developments of precision and even personalized therapy by the fast-growing field of probiogenomics.

DRUG INDUCED LIVER INJURY: A SYSTEMATIC REVIEW

Drug-induced liver injury (DILI), which has a variety of pathophysiological characteristics, is an important clinical problem. This review offers a thorough analysis of DILI, covering its pathophysiological mechanisms, the interaction between the microbiota and liver disease, the potential benefit of probiotics in preventing or treating DILI, diagnostic and prognostic methods, risk factors for DILI, current prevalence rates, and available treatments. The emergence of liver damage is influenced by the complex interplay of drug metabolism, immunological responses, and hereditary variables. An extensive list of medications, nutritional supplements, herbal products, and chemotherapeutic medicines that have been linked to DILI is provided based on the various research papers. The impact of the gut microbiota on healthy liver function and liver disease has been clarified by recent studies. Drug metabolism, immunological responses, and hepatic inflammation can all be affected by changes in gut microbial makeup and function, thereby worsening DILI. Probiotics’ impact on the gut-liver axis and their potential therapeutic advantages in the prevention and treatment of DILI are investigated. For effective management of DILI, a precise diagnosis and prognosis are essential. The outcomes of patients are predicted and treatment choices are guided by prognostic variables, such as liver function tests and drug causality evaluation tools. Inherited liver illnesses, genetic differences, patient characteristics, concurrent medication use, and other risk factors all have a role in the development of DILI. Identification of those at higher risk and the facilitation of specialized preventative interventions and patient monitoring is made possible by the recognition of these risk factors. DILI prevalence rates differ between populations and geographical areas. Planning for public health and allocating resources depend on tracking the incidence and prevalence of DILI. Although supportive care and medication withdrawal are the mainstays of DILI treatment, severe cases may call for a liver transplant. As a result, DILI is a multifactorial disorder that necessitates a thorough understanding of its processes, risk factors, diagnosis, and therapy. New insights into the gut microbiota and the possible impact of probiotics present intriguing directions for further study and treatment approaches to reduce liver damage caused by DILI.

Assessment of knowledge, attitudes, and practices regarding microbiota composition and influencing factors among the general population in Jazan province: A cross-sectional study.

The human gut microbiota is crucial to maintaining health and preventing diseases. The general population's knowledge, attitudes, and practices regarding microbiota composition and the factors affecting it remain poorly understood in Saudi Arabia. The present cross-sectional study aimed to evaluate the level of knowledge, attitudes, and practices among the general population in the Jazan Province of Saudi Arabia regarding the gut microbiota and its main influencing factors. A descriptive, cross-sectional design was employed, utilizing a validated self-administered online questionnaire to collect data from participants aged 18 years and older. The study population excluded nonresidents of the Jazan region, individuals below 18, and those who declined to participate. Nonrandomized convenience sampling was used to recruit participants from the Jazan Province, targeting the general population. One thousand one hundred twenty-six participants completed the survey, resulting in a response rate of 93%. Most participants (50.4%) had average microbiota knowledge, 14.7% demonstrated good understanding, and 34.9% had poor knowledge. Regarding probiotics, the findings indicated that 76.4% of participants exhibited insufficient knowledge, 21.1% had average knowledge, and 2.5% displayed good knowledge. Most participants (65.7%) held a neutral attitude toward antibiotics and probiotics. This study highlights a significant gap in understanding probiotics among the general population in the Jazan Region. Comprehensive education and awareness campaigns are urgently needed to promote a better understanding of microbiota composition, its significance for health, and the potential benefits of probiotics. Effective public health initiatives should be developed to provide accurate and up-to-date probiotic information, fostering positive health outcomes.

Heat-killed Limosilactobacillus reuteri ATCC PTA 6475 prevents bone loss in ovariectomized mice: A preliminary study.

Osteoporosis is an important health problem that occurs due to an imbalance between bone formation and resorption. Hormonal deficiency post-menopause is a significant risk factor. The probiotic Limosilactobacillus reuteri has been reported to prevent ovariectomy (Ovx)-induced bone loss in mice and reduce bone loss in postmenopausal women. Despite the numerous health benefits of probiotics, as they are live bacteria, the administration is not risk-free for certain groups (e.g., neonates and immunosuppressed patients). We evaluated the effects of L. reuteri (ATCC PTA 6475) and its heat-killed (postbiotic) form on Ovx-induced bone loss. Adult female mice (BALB/c) were randomly divided into four groups: group C-control (sham); group OVX-C-Ovx; group OVX-POS-Ovx + heat-killed probiotic; group OVX-PRO-Ovx + probiotic. L. reuteri or the postbiotic was administered to the groups (1.3x109 CFU/day) by gavage. Bacterial morphology after heat treatment was accessed by scanning electron microscopy (SEM). The treatment started one week after Ovx and lasted 28 days (4 weeks). The animals were euthanized at the end of the treatment period. Bone microarchitecture and ileum Occludin and pro-inflammatory cytokines gene expression were evaluated by computed microtomography and qPCR techniques, respectively. The Ovx groups had lower percentage of bone volume (BV/TV) and number of bone trabeculae as well as greater total porosity compared to the control group. Treatment with live and heat-killed L. reuteri resulted in higher BV/TV and trabecular thickness than the Ovx group. The heat treatment caused some cell surface disruptions, but its structure resembled that of the live probiotic in SEM analysis. There were no statistical differences in Occludin, Il-6 and Tnf-α gene expression. Both viable and heat-killed L. reuteri prevented bone loss on ovariectomized mice, independently of gut Occludin and intestinal Il-6 and Tnf-α gene expression.

Evidence on the benefits of probiotics for preterm infants.

This article reviews the evidence for the use of different strains of probiotics in the prevention of prevalent pathologies in premature neonates. A systematic review was conducted of the use of probiotics in neonates with less than 37 weeks of gestational age, based on a search for systematic reviews and observational and experimental studies performed during the period from January 2014 to February 2021. For this purpose, the PubMed, MEDLINE and Cochrane Library databases were consulted. The aim of this article was to review the existing data on the relationship between the administration of probiotics (with different strains and doses) and the risk of necrotising enterocolitis, mortality, late sepsis and other disease parameters in premature infants. The literature search obtained 240 articles, of which we selected 16, representing a total sample of over 200,000 premature infants. Analysis of the data obtained reveals statistical evidence that the combined administration of probiotics (especially of Lactobacillus and Bifidobacterium strains) reduces the incidence of grade II or higher necrotising enterocolitis, all-cause mortality, late sepsis, length of hospital stay and time until complete enteral nutrition is achieved. However, no benefits were apparent with respect to alleviating bronchopulmonary dysplasia, retinopathy of prematurity or intraventricular haemorrhage. Further research is needed to determine the most appropriate strains, doses and treatment duration for preterm infants to achieve the health benefits identified.

Both live and heat killed Lactiplantibacillus plantarum DPUL-F232 alleviate whey protein-induced food allergy by regulating cellular immunity and repairing the intestinal barrier.

Postbiotics have been proposed as clinically viable alternatives to probiotics, addressing limitations and safety concerns associated with probiotic use. However, direct comparisons between the functional differences and health benefits of probiotics and postbiotics remain scarce. This study compared directly the desensitization effect of probiotics and postbiotics derived from Lactiplantibacillus plantarum strain DPUL-F232 in the whey protein-induced allergic rat model. The results demonstrate that administering both live and heat killed F232 significantly alleviated allergy symptoms, reduced intestinal inflammation, and decreased serum antibody and histamine levels in rats. Both forms of F232 were effective in regulating the Th1/Th2 balance, promoting the secretion of the regulatory cytokine IL-10, inhibiting mast cell degranulation and restoring the integrity of the intestinal barrier through the upregulation of tight junction proteins. Considering the enhanced stability and reduced safety concerns of postbiotics compared to probiotics, alongside their ability to regulate allergic reactions, we suggest that postbiotics may serve as viable substitutes for probiotics in managing food allergies and potentially other diseases.

Exploring the Potential Benefits of Probiotics in Managing Periodontal Disease and Promoting Oral Health

Abstract Background: Periodontal disease is a chronic inflammatory condition that affects the supporting structures of teeth, leading to tissue destruction and tooth loss if left untreated. Traditional nonsurgical treatments, such as scaling and root planing (SRP), are effective but often fail to fully eradicate pathogenic bacteria, leading to disease recurrence. Probiotics, which are beneficial microorganisms, have emerged as a potential adjunctive therapy for managing periodontal disease by modulating the oral microbiome and reducing inflammation. Objective: To evaluate the clinical and microbiological outcomes of probiotic supplementation in conjunction with standard periodontal therapy in patients with chronic periodontitis. Materials and Methods: A randomized, double-blind, placebo controlled trial was conducted with 128 participants diagnosed with moderate-to-severe chronic periodontitis. Participants were randomly assigned into two groups: Group 1 (Probiotics + SRP, n = 64) and Group 2 (Placebo + SRP, n = 64). The clinical outcomes, including probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP), were measured at baseline and 3 months posttreatment. Subgingival microbiome composition was assessed using 16S rRNA sequencing. Statistical analysis was performed using the independent t-tests and Chi-square tests. Results: The probiotics group showed significant improvements in PD, CAL, and BOP compared to the placebo group (P &lt; 0.05). The microbiome analysis revealed a significant reduction in periodontal pathogens and an increase in beneficial bacteria in the probiotics group (P &lt; 0.05). Patient-reported outcomes also indicated better oral health-related quality of life in the probiotics group. Conclusion: Probiotic supplementation as an adjunct to standard periodontal therapy significantly enhances clinical outcomes and positively alters the subgingival microbiome in patients with chronic periodontitis. Probiotics offer a promising strategy for managing periodontal disease and promoting oral health.