Behavioral Traits Research Articles

Neuronal Remodelling as a Predictor of Autism Spectrum Disorder Diagnosis

People with Autism Spectrum Disorder (ASD) are a neurodevelopmental symptom group characterized by significant deficits in information connectivity, mainly caused by specific genetic mutations affecting the fluency of the gamma-aminobutyric acid (GABAergic) system. This disruption, to a greater or lesser degree, hinders the interactive process of incoming stimuli with previously stored information, preventing working memory from fulfilling its role of facilitating access to information in permanent memory and later recovery. In addition to the genetic components, environmental processes occurring, above all, in early childhood, related to specific organic and severe psycho-emotional diseases can produce the same effects in the brain system as the genetic karyotypic component through the concept of neuronal remodelling. In this sense, this research aims to empirically show the influence of neuronal remodelling on the GABAergic pathway leading to the traits of autism spectrum behaviour. A total of 175 participants from the three levels of ASD intensity have participated in this study. Data have been analysed through Linear Logistic Regression, which shows that both components, the genetic process and the environmental process, significantly influence the neural connective pathway that relates information, unlike other operationalized covariates, such as gender or age of the participants. Likewise, as shown by the Chi-Square Test, the interactions between genetic and environmental variables showed no differences in their interaction regarding the connectivity level that would explain the different levels of disorder. However, when there is a combination of genetic mutations in the diagnostic karyotype, there are significant differences between the genetic group and the environmental process over the neural network.

Interpopulation variation in dispersal behavior of fat minnow Rhynchocypris oxycephalus jouyi juveniles inhabiting fragmented habitats.

In many organisms, including fishes, downstream dispersal is often phenotype-dependent. Phenotype-dependent downstream dispersal can generate evolutionary pressure via spatial sorting, which non-randomly removes phenotypes enhancing downstream dispersal from upstream populations. Spatial sorting due to downstream dispersal could accumulate in fish populations in above-barrier habitats to which dispersed individuals cannot return, resulting in functional traits that reduce downstream dispersal. This evolutionary mechanism may be more important in smaller above-barrier habitats where downstream emigration occurs over shorter dispersal distances. This study observed the dispersal behavior of fat minnow Rhynchocypris oxycephalus jouyi juveniles in an experimental tank to examine whether juveniles from small above-barrier habitats show more behaviors favorable for reducing downstream dispersal than those from large above-barrier habitats. Juveniles from small above-barrier habitats avoided downstream dispersal for longer durations than those from large above-barrier habitats, but there was no difference in the frequency of ascending attempts. These results support the notion that behavioral traits of juveniles in small above-barrier populations have been refined by spatial sorting to reduce downstream dispersal. The finding that interpopulation variation in dispersal behavior occurred only for certain dispersal directions implies that the behavioral consequences of habitat fragmentation are more complex than previously assumed.

Long-term effects of widespread pharmaceutical pollution on trade-offs between behavioural, life-history and reproductive traits in fish.

In our rapidly changing world, understanding how species respond to shifting conditions is of paramount importance. Pharmaceutical pollutants are widespread in aquatic ecosystems globally, yet their impacts on animal behaviour, life-history and reproductive allocation remain poorly understood, especially in the context of intraspecific variation in ecologically important traits that facilitate species' adaptive capacities. We test whether a widespread pharmaceutical pollutant, fluoxetine (Prozac), disrupts the trade-off between individual-level (co)variation in behavioural, life-history and reproductive traits of freshwater fish. We exposed the progeny of wild-caught guppies (Poecilia reticulata) to three field-relevant levels of fluoxetine (mean measured concentrations: 0, 31.5 and 316 ng/L) for 5 years, across multiple generations. We used 12 independent laboratory populations and repeatedly quantified activity and risk-taking behaviour of male guppies, capturing both mean behaviours and variation within and between individuals across exposure treatments. We also measured key life-history traits (body condition, coloration and gonopodium size) and assessed post-copulatory sperm traits (sperm vitality, number and velocity) that are known to be under strong sexual selection in polyandrous species. Intraspecific (co)variation of these traits was analysed using a comprehensive, multivariate statistical approach. Fluoxetine had a dose-specific (mean) effect on the life-history and sperm trait of guppies: low pollutant exposure altered male body condition and increased gonopodium size, but reduced sperm velocity. At the individual level, fluoxetine reduced the behavioural plasticity of guppies by eroding their within-individual variation in both activity and risk-taking behaviour. Fluoxetine also altered between-individual correlations in pace-of-life syndrome traits: it triggered the emergence of correlations between behavioural and life-history traits (e.g. activity and body condition) and between life-history and sperm traits (e.g. gonopodium size and sperm vitality), but collapsed other between-individual correlations (e.g. activity and gonopodium size). Our results reveal that chronic exposure to global pollutants can affect phenotypic traits at both population and individual levels, and even alter individual-level correlations among such traits in a dose-specific manner. We discuss the need to integrate individual-level analyses and test behaviour in association with life-history and reproductive traits to fully understand how animals respond to human-induced environmental change.

Effects of DNA Methylation of HPA-Axis Genes of F1 Juvenile Induced by Maternal Density Stress on Behavior and Immune Traits in Root Voles (Microtus oeconomus)-A Field Experiment.

The literature shows that maternal stress can influence behavior and immune function in F1. Yet, most studies on these are from the laboratory, and replicated studies on the mechanisms by which maternal stress drives individual characteristics are still not fully understood in wild animals. We manipulated high- and low-density parental population density using large-scale field enclosures and examined behavior and immune traits. Within the field enclosures, we assessed anti-keyhole limpet hemocyanin immunoglobulin G (anti-KLH IgG) level, phytohemagglutinin (PHA) responses, hematology, cytokines, the depressive and anxiety-like behaviors and prevalence and intensity of coccidial infection. We then collected brain tissue from juvenile voles born at high or low density, quantified mRNA and protein expression of corticotropin-releasing hormone (CRH) and glucocorticoid receptor gene (NR3C1) and measured DNA methylation at CpG sites in a region that was highly conserved with the prairie vole CRH and NR3C1 promoter. At high density, we found that the F1 had a lower DNA methylation level of CRH and a higher DNA methylation level of NR3C1, which resulted in an increase in the expression levels of the CRH mRNA and protein expression and further reduced the expression levels of the NR3C1 mRNA and protein expression, and ultimately led to have delayed responses to acute immobilization stress. Juvenile voles born at high density also reduced anti-KLH IgG levels and PHA responses, increased cytokines, and depressive and anxiety-like behaviors, and the effects further led to higher coccidial infection. From the perspective of population density inducing the changes in behavior and immunity at the brain level, our results showed a physiological epigenetic mechanism for population self-regulation in voles. Our results indicate that altering the prenatal intrinsic stress environment can fundamentally impact behavior and immunity by DNA methylation of HPA-axis genes and can further drive population fluctuations in wild animals.

Early social isolation disrupts adult personality expression in group-living mites.

Animal personalities are characterized by intra-individual consistency and consistent inter-individual variability in behaviour across time and contexts. Personalities abound in animals, ranging from sea anemones to insects, arachnids, birds, fish and primates, yet the pathways mediating personality formation and expression remain elusive. Social conditions during the early postnatal period are known determinants of mean behavioural trait expressions later in life, but their relevance in shaping personality trajectories is unknown. Here, we investigated the consequences of early social isolation on adult personality expression in plant-inhabiting predatory mites Phytoseiulus persimilis. These mites are adapted to live in groups. We hypothesized that transient experience of social isolation early in life, that is, deprivation of any social contact during a sensitive window in the post-hatching phase, has enduring adverse effects on adult personality expression. Newly hatched mites were transiently reared in isolation or in groups and tested as adults for repeatability of various within-group behaviours, such as movement patterns and mutual interactions including sociability, defined as the propensity to associate and interact benignly with conspecifics, and activity patterns when alone. Groups composed of individuals with the same or different early-life experiences were repeatedly videotaped and individual behaviours were automatically analysed using AnimalTA. Social experiences early in life had persistent effects on mean behavioural traits as well as adult personality expression, as measured by intraclass correlation coefficients (indicating repeatability). On average, isolation-reared females moved at higher speeds, meandered less, kept greater distances from others and had fewer immediate neighbours than group-reared females. Group-reared females were highly repeatable in inter-individual distance, moving speed, meandering and area explored, whereas isolation-reared females were repeatable only in the number of immediate neighbours. Activity, quantified as the proportion of time spent moving within groups, was only repeatable in group-reared females, whereas activity, quantified as the proportion of time spent moving when alone, was only repeatable in females reared in isolation. Strikingly, also the early-life experiences of male mates influenced personality expression of mated females, with isolation-reared males boosting the repeatability of behavioural traits of group-reared females. Overall, our study provides evidence that a transient phase of social isolation during a critical period early in life has lasting effects that extend into adulthood, impairing adult personality expression. These effects should cascade upward, changing the phenotypic composition and diversity within populations.

GWAS as a tool for detecting SNPs in cattle to study their relationship to reproduction, productivity, growth, behavior, diseases

The fundamental goal of animal husbandry is the cost-effective production of human food from healthy animals, which includes production, reproduction. The method of whole-genome association study (GWAS) is actively used in various fields, including agriculture. Genome-wide association analyzes were generated as an identifier for genomic variations associated with economically significant traits in different livestock species. This method of genomic selection provides new priorities for improving the productive and reproductive qualities of livestock.The purpose of this review article is a comprehensive analysis of the current state of GWAS in cattle, focusing on the identification of SNPs associated with reproduction, productivity, growth, behavior and genetically determined diseases. The scope of the article covers the study of GWAS results worldwide, both in dairy and beef cattle, with special emphasis on the identification of candidate genes, QTL and genome regions related to the direction of productivity. Additionally, the organization of this review will include a classification of GWAS results based on the specific traits studied, providing a comprehensive overview of the genetic determinants of reproduction, growth, behavior, and disease traits in cattle.

Temperature-mediated dynamics: Unravelling the impact of temperature on cuticular hydrocarbon profiles, mating behaviour, and life history traits in three Drosophila species

In a world grappling with climate change, understanding the enduring impact of changes in temperatures on insect adult traits is crucial. It is proposed that cold- and warm-adapted species exhibit specialized behavioural and physiological responses to their respective temperature ranges. In contrast, generalist species maintain more stable metabolic and developmental rates across a broader range of temperatures, reflecting their ability to exploit diverse thermal niches. Here, we explored this intricate response to temperature exposure in three Drosophila species: Drosophila ezoana originating in Arctic regions, D. novamexicana in arid, hot environments, and in the cosmopolitan species D. virilis. Rearing these flies at 15, 20, 25, and 30 °C revealed striking variations in their cuticular hydrocarbon (CHC) profiles, known to mediate mate recognition and prevent water loss in insects. The cold-adapted D. ezoana consistently exhibited reduced CHC levels with increasing temperatures, while the warm-adapted D. novamexicana and the cosmopolitan D. virilis displayed more nuanced responses. Additionally, we observed a significant influence of rearing temperature on the mating behaviour of these flies, where those reared at the extreme temperatures, 15 and 30 °C, exhibiting reduced mating success. Consequently, this led to a decrease in the production of adult offspring. Also, these adult offspring underwent notable alterations in life history traits, reaching adulthood more rapidly at 25 and 30 °C but with lower weight and reduced longevity. Furthermore, among these offspring, those produced by the cold-adapted D. ezoana were more vulnerable to desiccation and starvation than those from the warm-adapted D. novamexicana and the cosmopolitan D. virilis. In summary, our research demonstrates that Drosophila species from diverse ecological regions exhibit distinct responses to temperature changes, as evidenced by variations in CHC profiles, mating behaviours, fertility, and life history traits. This provides valuable insights into how environmental conditions shape the biology and ecology of insects.

Mapping neural correlates of biological motion perception in autistic children using high-density diffuse optical tomography

BackgroundAutism spectrum disorder (ASD), a neurodevelopmental disorder defined by social communication deficits plus repetitive behaviors and restricted interests, currently affects 1/36 children in the general population. Recent advances in functional brain imaging show promise to provide useful biomarkers of ASD diagnostic likelihood, behavioral trait severity, and even response to therapeutic intervention. However, current gold-standard neuroimaging methods (e.g., functional magnetic resonance imaging, fMRI) are limited in naturalistic studies of brain function underlying ASD-associated behaviors due to the constrained imaging environment. Compared to fMRI, high-density diffuse optical tomography (HD-DOT), a non-invasive and minimally constraining optical neuroimaging modality, can overcome these limitations. Herein, we aimed to establish HD-DOT to evaluate brain function in autistic and non-autistic school-age children as they performed a biological motion perception task previously shown to yield results related to both ASD diagnosis and behavioral traits.MethodsWe used HD-DOT to image brain function in 46 ASD school-age participants and 49 non-autistic individuals (NAI) as they viewed dynamic point-light displays of coherent biological and scrambled motion. We assessed group-level cortical brain function with statistical parametric mapping. Additionally, we tested for brain-behavior associations with dimensional metrics of autism traits, as measured with the Social Responsiveness Scale-2, with hierarchical regression models.ResultsWe found that NAI participants presented stronger brain activity contrast (coherent > scrambled) than ASD children in cortical regions related to visual, motor, and social processing. Additionally, regression models revealed multiple cortical regions in autistic participants where brain function is significantly associated with dimensional measures of ASD traits.LimitationsOptical imaging methods are limited in depth sensitivity and so cannot measure brain activity within deep subcortical regions. However, the field of view of this HD-DOT system includes multiple brain regions previously implicated in both task-based and task-free studies on autism.ConclusionsThis study demonstrates that HD-DOT is sensitive to brain function that both differentiates between NAI and ASD groups and correlates with dimensional measures of ASD traits. These findings establish HD-DOT as an effective tool for investigating brain function in autistic and non-autistic children. Moreover, this study established neural correlates related to biological motion perception and its association with dimensional measures of ASD traits.

Side effects of five products largely used by humans and studied on ants as models: a mini review

Here we summarize our recent studies on the physiological and behavior effects of five products widely used by humans, using an ant, having received these substances in their usual diet, as a biological model. Nutmeg is a spice which decrease the activity and movement speed of the ants, leading to further motor-linked impairments, but not impairing other behavioral traits as well as learning and memory. Vortioxetine (Brintellix) is an anti-depressant recently appearing reducing obesity and having moderate adverse effects in humans. It negatively affected the ants’ food intake, general activity, tactile sensitivity, state of stress and cognition. Berberine is a supplementary dietary regulating, in humans, glucosemia and several parameters. In ants, food intake, general activity, tactile sensitivity, were also negatively impacted, but not their state of stress and cognition. Cashew nuts and mangoes may have similar allergenic effects in humans. In ants, cashew consumption induces sinuosity of movement, stress and nervousness while mango consumption reduces their general activity and behaviors related to motor activity. The correspondence between dependence to a substance, short duration of its effect after withdrawal and abrupt loss of its effect is discussed.

Using joint species distribution modelling to identify climatic and non‐climatic drivers of Afrotropical ungulate distributions

The relative importance of the different processes that determine the distribution of species and the assembly of communities is a key question in ecology. The distribution of any individual species is affected by a wide range of environmental variables as well as through interactions with other species; the resulting distributions determine the pool of species available to form local communities at fine spatial scales. A challenge in community ecology is that these interactions (e.g. competition, facilitation, etc.) often are not directly measurable. Here, we used hierarchical modelling of species communities (HMSC), a recently developed framework for joint species distribution modelling, to estimate the role of biotic effects alongside environmental factors using latent variables. We investigate the role of these factors determining species distributions in communities of Artiodactyla, Perissodactyla and Proboscidea in the Afrotropics, an area of peak species richness for hoofed mammals. We also calculate pairwise trait dissimilarity between these species, from a mixture of morphological and behavioural traits, and investigate the relationship between dissimilarity and estimated residual co‐occurrence in the model. We find that while ungulate distributions appear to be predominantly determined (~ 70%) by climatic variables, such as precipitation, a substantial proportion of the variance in ungulate species distributions (~ 30%) can also be attributed to modelled latent variables that likely represent a combination of dispersal barriers and biotic factors. Although we find only a weak relationship between residual co‐occurrence and trait dissimilarity, we suggest that our results may show evidence that biotic factors, likely influenced by historical barriers to species dispersal, are important in determining species communities over a continental area. The HMSC framework can be used to provide insight into factors affecting community assembly at broad scales, and to make more powerful predictions about future species distributions as we enter an era of increasing impacts from anthropogenic change.

Dissociable Roles of the mPFC-to-VTA Pathway in the Control of Impulsive Action and Risk-Related Decision-Making in Roman High- and Low-Avoidance Rats.

Impulsive action and risk-related decision-making (RDM) are associated with various psychiatric disorders, including drug abuse. Both behavioral traits have also been linked to reduced frontocortical activity and alterations in dopamine function in the ventral tegmental area (VTA). However, despite direct projections from the medial prefrontal cortex (mPFC) to the VTA, the specific role of the mPFC-to-VTA pathway in controlling impulsive action and RDM remains unexplored. We used positron emission tomography with [18F]-fluorodeoxyglucose to evaluate brain metabolic activity in Roman high- (RHA) and low-avoidance (RLA) rats, which exhibit innate differences in impulsive action and RDM. Notably, we used a viral-based double dissociation chemogenetic strategy to isolate, for the first time to our knowledge, the role of the mPFC-to-VTA pathway in controlling these behaviors. We selectively activated the mPFC-to-VTA pathway in RHA rats and inhibited it in RLA rats, assessing the effects on impulsive action and RDM in the rat gambling task. Our results showed that RHA rats displayed higher impulsive action, less optimal decision-making, and lower cortical activity than RLA rats at baseline. Chemogenetic activation of the mPFC-to-VTA pathway reduced impulsive action in RHA rats, whereas chemogenetic inhibition had the opposite effect in RLA rats. However, these manipulations did not affect RDM. Thus, by specifically targeting the mPFC-to-VTA pathway in a phenotype-dependent way, we reverted innate patterns of impulsive action but not RDM. Our findings suggest a dissociable role of the mPFC-to-VTA pathway in impulsive action and RDM, highlighting its potential as a target for investigating impulsivity-related disorders.

The Ginkgo—Covid connection

Social Impact StatementGingko biloba is an iconic tree with a surprising connection to the SARS and Covid viruses that have impacted human health since 2003. Natural history studies on the ecology of Gingko seed dispersal by Carnivoran scavengers in Asia over the past 30 plus years have elucidated the deep evolutionary history that not only makes these animals effective at spreading seeds but also predisposes them to become intermediate hosts for spreading diseases to humans. Understanding the behavioral traits that underlie these kinds of plant–animal interactions has the potential to help people limit the spread of future spillover diseases.SummaryRaccoon dogs and masked palm civets are Carnivoran scavengers native to Eastern Asia that have been documented feeding on the malodorous sarcotesta of Ginkgo biloba seeds and effectively dispersing the hard nuts they contain. Research has shown that their immune systems are genetically enhanced to protect them from the disease‐causing organisms they might pick up from their omnivorous diets that include live and dead animals. Both species are farmed for fur and meat across China and, up until recently, were commonly sold in “wet” markets. Research has determined that the palm civet played a role in spreading the SARS virus to humans in 2003 and that the raccoon dog may have played a similar role in spreading Covid in Wuhan in 2019, although this has not been proven. In areas where Ginkgo is cultivated, there are numerous internet reports of Carnivorans feeding on its seeds indicating that its dispersal strategy of attracting scavengers by mimicking the smell of carrion is adaptive and effective.

Exposure to thimerosal induces behavioral abnormality in the early life stages of zebrafish via altering amino acid homeostasis

Thimerosal (THI) has become a significant source of organic mercury pollutants in aquatic ecosystems, but there is limited information regarding its adverse effects on fish. In this study, zebrafish embryos were exposed to THI at 0 (control), 5.0, and 50 ng/L from 0–5 days post fertilization (dpf), and variations in their survival, development, behavior, free amino acid contents, and the biochemical responses involved in monoaminergic systems were examined. Although THI exposure did not significantly affect the survival, heart rate, or hatching time of zebrafish embryos, it substantially increased swimming velocity (136–154 % of the control) and reduced exploratory behavior (141–142 % of the control) in zebrafish larvae at 5 dpf. Exposure also significantly altered the amino acid contents (51–209 % of the control) and monoamine levels (70–154 % of the control) in zebrafish larvae, some of which displayed significant correlations with behavioral traits. THI significantly elevated dopamine receptor gene expression and monoamine oxidase activity in zebrafish larvae. Adding extra phenylalanine or tryptophan to the E3 medium facilitates the recovery of zebrafish larvae from the abnormal behaviors induced by THI. These findings reveal for the first time that THI exposure at the level of ng/L is sufficient to induce neurobehavioral toxic effects in the early life stages of zebrafish, and disrupting amino acid homeostasis is a critical underlying mechanism. This study provides valuable insights into the toxicity of THI to fish and highlights the importance of assessing its potential risks to aquatic ecosystems.

The effect of a trampoline exercise program on motor and behavioral characteristics of adolescents with autism spectrum disorder

Purpose The aim of this study is to evaluate the impact of a trampoline intervention program on motor and behavioral characteristics of adolescents with autism spectrum disorder (ASD). Design/methodology/approach Twenty-six male students aged 15–18 years with ASD participated in the study. They were equally divided into two groups of 13 students. The intervention group followed an individualized trampoline exercise program that was applied three times a week for 30 min for 12 weeks. Before the intervention program and after the end of this, the motor performance was evaluated through the recording of the total number of jumps. The behavioral characteristics were evaluated with the Behavior Problem Inventory questionnaire. Findings According to the findings, there were statistically significant differences between the intervention group and the control group across all variables. In conclusion, it seems that an intervention program with the use of a trampoline, adapted to the needs and capabilities of adolescents with ASD can improve their motor performance and behavioral characteristics. Originality/value The authors report on the impact of a trampoline exercise program on motor skills and behavioral traits in adolescents diagnosed with ASD. This is significant because our research focused on investigating the effects of a structured trampoline exercise program both on motor skills and behavioral characteristics. The findings contribute to the growing body of evidence supporting targeted exercise programs as effective interventions for addressing motor and behavioral challenges in adolescents with ASD. The authors believe the research aligns closely with the aims and scope of research in developmental disabilities, and the authors hope that it will make a meaningful impact on the field of autism research and intervention.

Proximity to humans is associated with antimicrobial-resistant enteric pathogens in wild bird microbiomes

Humans are radically altering global ecology, and one of the most apparent human-induced effects is urbanization, where high-density human habitats disrupt long-established ecotones. Changes to these transitional areas between organisms, especially enhanced contact among humans and wild animals, provide new opportunities for the spread of zoonotic pathogens. This poses a serious threat to global public health, but little is known about how habitat disruption impacts cross-species pathogen spread. Here, we investigated variation in the zoonotic enteric pathogen Campylobacter jejuni. The ubiquity of C.jejuni in wild bird gut microbiomes makes it an ideal organism for understanding how host behavior and ecology influence pathogen transition and spread. We analyzed 700 C.jejuni isolate genomes from 30 bird species in eight countries using a scalable generalized linear model approach. Comparing multiple behavioral and ecological traits showed that proximity to human habitation promotes lineage diversity and is associated with antimicrobial-resistant (AMR) strains in natural populations. Specifically, wild birds from urban areas harbored up to three times more C.jejuni genotypes and AMR genes. This study provides novel methodology and much-needed quantitative evidence linking urbanization to gene pool spread and zoonoses.

Description and predictors of self-determination in males and females with fragile X syndrome on the verge of adulthood.

Self-determination refers to setting goals and making decisions regarding one's own life with support from others as needed. Research on people with intellectual and developmental disabilities has established the importance of self-determination for quality of life outcomes, such as increased independence and life satisfaction. However, self-determination has not been characterised specifically in fragile X syndrome (FXS), the leading inherited cause of intellectual disability. Relative to youth with other forms of intellectual and developmental disabilities, youth with FXS may face exceptional barriers to the development of self-determined behaviour. In addition to intellectual disability, the FXS behavioural profile is characterised by high rates of autism and anxiety that may further limit opportunities for youth with FXS. The heritable nature of the condition can also yield a distinctive family environment, with siblings and parents also living with fragile X or its associated conditions. Considering these unique challenges, the present study examined self-determination in young adult males and females with FXS and explored whether factors such as language skills, adaptive behaviour and autism traits were associated with self-determination capacity and opportunities. The present study included 9 females and 36 males with FXS between the ages of 17 and 25years. Caregivers (mothers or fathers) completed the American Institute for Research Self-Determination Assessment, which is a questionnaire that yields three scores: self-determination capacity, opportunities for self-determination at home and opportunities for self-determination at school. Caregivers endorsed a wide range of self-determination capacity and opportunities, with ratings for opportunities at home and school exceeding ratings of capacity. Better adaptive behaviour skills were associated with more self-determination capacity, and the presence of more autism traits was associated with fewer opportunities at school. Results from this study contribute to our understanding of avenues to best support young adults with FXS as they transition to adulthood. Our findings also have implications for practice, such that interventions targeting adaptive behaviours and self-determination may be an effective approach for promoting autonomy and independence for young adults with FXS. Additionally, caregivers and educators should continue to provide opportunities to practise self-determination, regardless of their perception of capacity.

Precision medicine for obesity: current evidence and insights for personalization of obesity pharmacotherapy.

Obesity is a chronic and complex disease associated with increased morbidity, mortality, and financial burden. It is expected that by 2030 one of two people in the United States will have obesity. The backbone for obesity management continues to be lifestyle interventions, consisting of calorie deficit diets and increased physical activity levels, however, these interventions are often insufficient to achieve sufficient and maintained weight loss. As a result, multiple patients require additional interventions such as antiobesity medications or bariatric interventions in order to achieve clinically significant weight loss and improvement or resolution of obesity-associated comorbidities. Despite the recent advances in the field of obesity pharmacotherapy that have resulted in never-before-seen weight loss outcomes, comorbidity improvement, and even reduction in cardiovascular mortality, there is still a significant interindividual variability in terms of response to antiobesity medications, with a subset of patients not achieving a clinically significant weight loss. Currently, the trial-and-error paradigm for the selection of antiobesity medications results in increased costs and risks for developing side effects, while also reduces engagement in weight management programs for patients with obesity. The implementation of a precision medicine framework to the selection of antiobesity medications might help reduce heterogeneity and optimize weight loss outcomes by identifying unique subsets of patients, or phenotypes, that have a better response to a specific intervention. The detailed study of energy balance regulation holds promise, as actionable behavioral and physiologic traits could help guide antiobesity medication selection based on previous mechanistic studies. Moreover, the rapid advances in genotyping, multi-omics, and big data analysis might hold the key to discover additional signatures or phenotypes that might respond better to a certain intervention and might permit the widespread adoption of a precision medicine approach for obesity management.

Interactions between migration and immunity among oriental armyworm populations infected with the insect pathogenic fungus, Beauveria bassiana.

Migration and immunity are behavioral and physiological traits that protect organisms from environmental stressors or pathogen infection. Shifting from migration to residency has become more common in some wildlife populations owing to environmental changes. However, other biological shifts, such as interactions between migration and immunity among populations within a species are largely unexplored for many agricultural migratory pests. In the field, entomopathogenic fungi infection and transmission, particularly Beauveria bassiana, can cause reduced fitness and population declines across a broad range of insect species. Here, we investigated migration-immunity interactions between migrant and resident populations of the oriental armyworm, Mythimna separata, infected with B. bassiana (the sole fungus used in this work). We found that migratory M. separata exerted stronger pathogen resistance, faster development and lower pupal weight than residents. High-dose infections (5.0 × 105 and 5.0 × 106 conidia mL-1) led to seriously decreased reproductive capacity in migrants and residents. Low-dose infections (1.0 × 104 and 5.0 × 104 conidia mL-1) led to significantly increased host flight capacities. Consecutive flight tests showed that five flight nights inhibited the reproduction of paternal infected M. separata populations. The flights also led to far-reaching transgenerational impairment of larval development and immune defense among offspring populations. By contrast, two flight nights enhanced the reproductive capacities of both M. separata populations and did not exert negative transgenerational effects on offspring populations, which may facilitate migration. This study provides insights into interactions between migration and immunity among M. separata populations. These insights will guide development of future monitoring and management technologies of this pest. © 2024 Society of Chemical Industry.

Examining the relationship between functional connectivity and broader autistic traits in non-autistic children

ABSTRACT In the current study, we used functional near-infrared spectroscopy (fNIRS) to examine functional connectivity (FC) in relation to measures of cognitive flexibility and autistic features in non-autistic children. Previous research suggests that disruptions in FC between brain regions may underlie the cognitive and behavioral traits of autism. Moreover, research has identified a broader autistic phenotype (BAP), which refers to a set of behavioral traits that fall along a continuum of behaviors typical for autism but which do not cross a clinically relevant threshold. Thus, by examining FC in relation to the BAP in non-autistic children, we can better understand the spectrum of behaviors related to this condition and their neural basis. Results indicated age-related differences in performance across three measures of cognitive flexibility, as expected given the rapid development of this skill within this time period. Additionally, results showed that across the flexibility tasks, measures of autistic traits were associated with weaker FC along the executive control network, though task performance was not associated with FC. These results suggest that behavioral scores may be less sensitive than neural measures to autistic traits. Further, these results corroborate the use of broader autistic traits and the BAP to better understand disruptions to neural function associated with autism.

Field Exploration for Colony Selection: Evaluating Hygienic Behavior in Apis cerana indica Colonies.

Hygienic behavior (HB) emerges as a pivotal trait, impacting colony resistance to diseases. This study aimed to understand the behavioral traits of Apis cerana indica colonies, with a focus on HB and other key characteristics crucial for colony health, and to screen and identify colonies with superior hygienic behavior and better performance to combat prevailing diseases and pests. This research spans a comprehensive field analysis with different seasons and locations, encompassing the distinct environmental and management factors that influence honey bee behavior. The inclusion of A. cerana indica colonies from various locations provides a novel perspective, offering valuable insights regarding the hygienic behavior of A. cerana indica. Several statistical analyses, including descriptive statistics, principal component analysis (PCA), and Aligned Rank Transformation-Analysis of Variance (ART-ANOVA) for repeated measures, shed light on the distribution of hive metrics, emphasizing the significance of considering seasonality and location-specific factors. PCA highlights unique characteristics in Tirupur and Coimbatore colonies, while correlation analyses uncover relationships among HB, honey, pollen, brood area, and adult population. Moreover, the study's nuanced findings gave the status of hygienic behavior of A. cerana indica colonies and identified colonies with better colony performance, which will be useful for future breeding programs with A. cerana indica.