Behavioral Diversity Research Articles

Pharmacological and behavioral divergence of ketamine enantiomers: implications for abuse liability.

Ketamine, a racemic mixture of (S)-ketamine and (R)-ketamine enantiomers, has been used as an anesthetic, analgesic and more recently, as an antidepressant. However, ketamine has known abuse liability (the tendency of a drug to be used in non-medical situations due to its psychoactive effects), which raises concerns for its therapeutic use. (S)-ketamine was recently approved by the United States’ FDA for treatment-resistant depression. Recent studies showed that (R)-ketamine has greater efficacy than (S)-ketamine in preclinical models of depression, but its clinical antidepressant efficacy has not been established. The behavioral effects of racemic ketamine have been studied extensively in preclinical models predictive of abuse liability in humans (self-administration and conditioned place preference [CPP]). In contrast, the behavioral effects of each enantiomer in these models are unknown. We show here that in the intravenous drug self-administration model, the gold standard procedure to assess potential abuse liability of drugs in humans, rats self-administered (S)-ketamine but not (R)-ketamine. Subanesthetic, antidepressant-like doses of (S)-ketamine, but not of (R)-ketamine, induced locomotor activity (in an opioid receptor-dependent manner), induced psychomotor sensitization, induced CPP in mice, and selectively increased metabolic activity and dopamine tone in medial prefrontal cortex (mPFC) of rats. Pharmacological screening across thousands of human proteins and at biological targets known to interact with ketamine yielded divergent binding and functional enantiomer profiles, including selective mu and kappa opioid receptor activation by (S)-ketamine in mPFC. Our results demonstrate divergence in the pharmacological, functional, and behavioral effects of ketamine enantiomers, and suggest that racemic ketamine’s abuse liability in humans is primarily due to the pharmacological effects of its (S)-enantiomer.

Flight behavior of four species of Holotrichia chafer (Coleoptera: Scarabaeidae) with different habitat use

Insect flight behavior varies among species and selective pressures underlying the diversification of flight performance remain to be fully elucidated. Comparisons of flight behavior among congeners with differing ecologies can contribute to gaining a better understanding of the mechanisms underlying the diversification of insect flight behavior. In this study, we compared the laboratory arena flight performance of four species of nocturnal Holotrichia black chafers (Coleoptera: Scarabaeidae) based on three-dimensional reconstructions of flight trajectories. Principal component analysis was applied to six kinematic parameters to summarize the flight performance of each species. We found that the flight of H. parallela, which feeds on the leaves of hardwood species, was faster and higher than that of the other three species (H. kiotonensis, H. picea, and H. convexopyga), which typically feed on herbaceous plants. However, maneuverability (rotational component) did not differ significantly among the four species. We further compared the flight velocities of H. parallela and H. kiotonensis using a tethered flight mill system. Similar to the free-flight observations, the velocity of H. parallela flights was significantly greater than that of H. kiotonensis. The interspecific difference in velocities may be associated with differences in spatial distribution of their host plants.

Accelerated Brain Shape Evolution Is Associated with Rapid Diversification in an Avian Radiation.

AbstractNiche expansion is a critical step in the speciation process. Large brains linked to improved cognitive ability may enable species to expand their niches and forage in new ways, thereby promoting speciation. Despite considerable work on ecological divergence in brain size and its importance in speciation, relatively little is known about how brain shape relates to behavioral, ecological, and taxonomic diversity at macroevolutionary scales. This is due in part to inherent challenges with quantifying brain shape across many species. Here we present a novel, semiautomated approach for rapidly phenotyping brain shape using semilandmarks derived from X-ray computed microtomography scans. We then test its utility by parsing evolutionary trends within a diverse radiation of birds: kingfishers (Aves: Alcedinidae). Multivariate comparative analyses reveal that rates of brain shape evolution (but not beak shape) are positively correlated with lineage diversification rates. Distinct brain shapes are further associated with changes in body size and foraging behavior, suggesting both allometric and ecological constraints on brain shape evolution. These results are in line with the idea of brains acting as a "master regulator" of critical processes governing speciation, such as dispersal, foraging behavior, and dietary niche.

Nongenetic individuality, changeability, and inheritance in bacterial behavior

Isogenic populations often display remarkable levels of phenotypic diversity even in constant, homogeneous environments. Such diversity results from differences between individuals ("nongenetic individuality") as well as changes during individuals' lifetimes ("changeability"). Yet, studies that capture and quantify both sources of diversity are scarce. Here we measure the swimming behavior of hundreds of Escherichia coli bacteria continuously over two generations and use a model-independent method for quantifying behavior to show that the behavioral space of E. coli is low-dimensional, with variations occurring mainly along two independent and interpretable behavioral traits. By statistically decomposing the diversity in these two traits, we find that individuality is the main source of diversity, while changeability makes a smaller but significant contribution. Finally, we show that even though traits of closely related individuals can be remarkably different, they exhibit positive correlations across generations that imply nongenetic inheritance. The model-independent experimental and theoretical framework developed here paves the way for more general studies of microbial behavioral diversity.

Welfare-Related Behaviors in Chickens: Characterization of Fear and Exploration in Local and Commercial Chicken Strains.

Simple SummaryLocally adapted chicken strains are frequently discussed as a favorable alternative in poultry production. They might serve as dual-purpose and robust strains that have advantageous behaviors, such as exploration and reduced fear reactions, which might support animal welfare in general. Therefore, it is important to characterize welfare-related behaviors, such as fear and exploration, and to identify these behavioral traits among the diversity of chicken strains. The aim of this study was to characterize fear reactions and exploration based on the avoidance of a novel object and the avoidance of a human in three different chicken strains. To identify interesting alternatives for future poultry production, a layer hybrid (Lohmann Brown), a dual-purpose hybrid (Lohmann Dual) and a locally adapted strain (Rhinelander) were tested for their fear and exploration behavior towards unknown objects and humans. Results showed clear differences in fear and exploration between all three strains, regarding fear of objects and humans. The strain-specific results illustrate the high diversity of behavioral traits. The understanding of behavioral diversity, including fear and exploration, might contribute substantially to future breeding programs, including of commercial strains, and the preservation of local strains in general, which can improve animal welfare on a genetic and individual basis.Fear and exploration are crucial traits determining how animals behave in novel situations, and thus, they influence animal welfare. The aim of this study was the characterization of these behavioral traits among different strains to identify interesting alternatives for future poultry production. Whereas the Novel Object Test (NOT) focuses on fear and exploration of novel objects, the Avoidance Distance Test (ADT) addresses this in the context of humans. Here, a commercial hybrid line, a dual-purpose hybrid and a local adapted strain were tested. For the differences between strains and development of fear, Lohmann Brown (n = 714), Lohmann Dual (n = 844) and Rhinelander (n = 458) were observed weekly until maturity. Results show that fear and exploration towards unknown objects and humans are breed-specific (all p < 0.01). Additionally, development of fear in NOT and ADT differed between all three strains (both p < 0.01). The expressions of fear of humans or objects should be regarded as characteristics adapted for different husbandry systems and breeding goals, e.g., high exploratory behavior in aviary or high avoidance of predators in free-ranging husbandry or at least a balanced ratio between fear and exploration. Characterization of behavioral traits among different strains, understanding diversity and integrating these behaviors into future breeding and husbandry systems might reflect the need to preserve local strains and the potential to improve animal welfare.

Comparative study on cyclic behavior of marine calcareous sand and terrigenous siliceous sand for transportation infrastructure applications

Calcareous sand has been used as an emerging unbound granular filler to replace siliceous sand in the construction of marine transportation infrastructure. However, the critical feasibility of and differences between using calcareous sand as a filler instead of siliceous sand have yet to be suitably assessed. In light of this, this paper presented a comparative study on the cyclic behavior of calcareous sand and siliceous sand through a series of high-cycle drained triaxial tests. To facilitate comparison, the same grain-size distribution and relative density were used for calcareous sand and siliceous sand to investigate any underlying cyclic behavior divergence caused by their respective distinctive particle shapes and breakage. Owing to the irregular and rough properties of calcareous sand particles, results showed that it exhibited stronger shear strength and resistance to permanent deformation than siliceous sand. However, during the early and middle stages of cyclic loading, the accumulation rate of permanent strain in calcareous sand was found to exceed that of siliceous sand due to its violent particle breakage habit, whereas a more severe dilatancy of calcareous sand induced by irregular particle shape was observed during the subsequent loading stage. Moreover, particle shape and particle breakage were found to have different competitive contributions to the increase of resilient modulus during different loading stages. In this work, the allowable cyclic strength ratio capable of better determining the suitable load level for a practical design was suggested. Furthermore, to capture the particle breakage extent under long-term cyclic loads, a refined hyperbolic equation was projected to model the relationship between relative particle breakage index and plastic work.

Behavioral Science and Diversity in Software Engineering

The “Practioners' Digest” department in this issue of IEEE Software covers two topics: the behavioral science of software engineering and diversity in software engineering (this issue’s theme) and includes papers from the 42nd International Conference on Software Engineering (ICSE20), 2019 IEEE International Conference on Software Maintenance and Evolution (ICSME19), 13th International Workshop on Cooperative and Human Aspects of Software Engineering (CHASE20), Empirical Software Engineering and Measurement 2020 (ESEM20), and Association for Computing Machinery Joint European Software Engineering Conference and Symposium on the Foundations of Software Engineering (ESEC/FSE20). Feedback or suggestions are welcome. In addition, if you try or adopt any of the practices included in this article, please send me and the authors of the paper(s) a note about your experiences.

Fishers as foragers: Individual variation among small-scale fishing vessels as revealed by novel tracking technology

Effective fisheries management requires an understanding of fisher behavior. Though vessel tracking systems are increasingly used to describe the movements and activities of industrial fishing fleets, their use has been limited within the small-scale fisheries employing the vast majority of the world’s capture fishers. Here we combine novel vessel tracking technology with logbook data to conduct a high-resolution analysis of behavior and decision-making within a small-scale fishery. Our results indicate significant heterogeneity in fisher behavior, catch composition, and profits within a small-scale fleet operating in the central Gulf of California, even amongst fishing vessels using similar gear types. The weekly home ranges (75 % Kernel Utilization Distribution) occupied by fishers spanned 1.5–1121.8 km2 across 13 vessels, while weekly profits ranged from -1810 to 26,160 pesos. Differences in the spatial interactions and catch profiles of observed vessels revealed the existence of behavioral associations linked with distinct fishing strategies. After identifying and describing the contextual factors driving such heterogeneity among vessels using hook and line fishing gear, we interpret emergent patterns and processes using insights from foraging theory and marine social science. In illustrating the applications and opportunities presented by recent advances in vessel tracking technology, we argue for the use of spatially explicit analytical approaches in assessing behavioral diversity within small-scale fisheries and in designing robust and equitable management strategies.

Correction to 'Behavioural hypervolumes of spider communities predict community performance and disbandment'.

Correction to 'Behavioural hypervolumes of spider communities predict community performance and disbandment'.

Spatio-temporal distribution of sexual calling behaviour in domesticated, sterile and wild Queensland fruit fly males under field cage conditions.

The sterile insect technique (SIT) is used in Australia to contain and eradicate outbreaks of Bactrocera tryoni (Froggatt) (Diptera: Tephritidae) in fruit-fly-free regions, and to manage populations in some endemic regions. To assess the likely impact of SIT on wild B. tryoni populations it is important to assess the colocation and synchrony of male calling between sterile and wild flies. We observed the location and timing of calling behaviours of marked mass-reared (fertile and sterile) and wild B. tryoni males in walk-in field cages. We found that wild males called further from the canopy centre than mass-reared (fertile or sterile) males. Mass-reared (fertile or sterile) males called earlier in the evening than wild males and, consequently, mass-reared males called when temperature and light intensity were higher than when wild males called. Male calling is a prerequisite to mating among dacine fruit flies. Therefore, our observations of spatio-temporal divergence in male calling behaviour may lead to assortative mating between mass-reared and wild B. tryoni in SIT applications. The importance of these spatio-temporal differences warrants further inquiry, with particular focus on how environmental conditions modify calling behaviour and avenues to ameliorate differences between sterile and wild flies. © 2021 Society of Chemical Industry.

Anthropogenically modified habitats favor bigger and bolder lizards.

Anthropogenic activities often create distinctive but discontinuously distributed habitat patches with abundant food but high risk of predation. Such sites can be most effectively utilized by individuals with specific behaviors and morphologies. Thus, a widespread species that contains a diversity of sizes and behavioral types may be pre‐adapted to exploiting such hotspots. In eastern Australia, the giant (to >2 m) lizard Varanus varius (lace monitor) utilizes both disturbed (campground) and undisturbed (bushland) habitats. Our surveys of 27 sites show that lizards found in campgrounds tended to be larger and bolder than those in adjacent bushland. This divergence became even more marked after the arrival of a toxic invasive species (the cane toad, Rhinella marina) caused high mortality in larger and bolder lizards. Some of the behavioral divergences between campground and bushland lizards may be secondary consequences of differences in body size, but other habitat‐associated divergences in behavior are due to habituation and/or nonrandom mortality.

Behavioural instability as an indicator of personality within captive populations of Rothschild Giraffes

The novel concept of behavioural instability has proven suitable for studying the behavior and personality in zoo animals. Individual personality has an impact on how the zoo best perform environmental enrichment, and behavioural diversity of captive populations intended to potentially repopulate wild habitats. This study aims to prove the presence of recognizable personalities in Rothschild giraffes (Giraffa camelopardalis rothschildi), as well as to investigate whether visitor numbers affect the behavioural expressions in this species. Six giraffes (bot young and adults) were filmed in Aalborg Zoo, and the recordings were subsequently analysed. The procured data was then analysed using a series of tests primarily focusing on behavioural reaction norms. The results shows that distinct personalities exist within the groups of Rothschild giraffes. However, the number of visitors exhibited no statistical significance upon the behaviour of the individuals. It can thereby be concluded that specimens of Rothschild giraffes possess unique personality traits which are worth defining in order to ensure behavioural diversity.

Personality shifts are inconsistent across two rapid range expansions of a non-native spider

Successful biological invasions are characterized by the spread of a population across a landscape. Range expansion conditions can favour different phenotypes at the leading edges of the range compared to those at the core. Although Cyrtophora citricola is a non-native spider species that was documented in Florida, U.S.A. almost two decades ago, the status of its populations remains unstudied. We report two populations that have spread northward along both eastern and western coasts from a putative common origin. We asked whether the leading edges of these two range expansions are composed of different personality types compared to the original core and whether core-to-edge patterns in personality are consistent between the two replicate populations. More voracious, more exploratory and bolder spiders were predicted to occupy the leading edge, because these personality types are assumed to be associated with dispersive, successful colonizers in novel environments. While leading-edge spiders were indeed faster to respond and attack prey stimuli and active longer in novel environments compared to spiders from the core, the two populations diverged in boldness. On average, western spiders were less bold and exploratory towards newer colonization sites, whereas eastern spiders were bolder at newer sites. The divergence in exploratory behaviour and boldness between these populations highlights the difficulties in generalizing how traits may become assorted across an expanding range and stress the importance of using replicate populations when possible. These results suggest that range level processes can be inconsistent and highlight the importance of investigating how the strength of local adaptation may swamp processes such as spatial sorting.

Extensive Linkage and Genetic Coupling of Song and Preference Loci Underlying Rapid Speciation in Laupala Crickets.

In nature, closely related species commonly display divergent mating behaviors, suggesting a central role for such traits in the origin of species. Elucidating the genetic basis of divergence in these traits is necessary to understand the evolutionary process leading to reproductive barriers and speciation. The rapidly speciating Hawaiian crickets of the genus Laupala provides an ideal system for dissecting the genetic basis of mating behavior divergence. In Laupala, closely related species differ markedly in male song pulse rate and female preference for pulse rate. These behaviors play an important role in determining mating patterns. Previous studies identified a genetic architecture consisting of numerous small to moderate effect loci causing interspecific differences in pulse rate and preference, including colocalizing pulse rate and preference QTL on linkage group one (LG1). To further interrogate these QTL, we conduct a fine mapping study using high-density SNP linkage maps. With improved statistical power and map resolution, we provide robust evidence for genetic coupling between song and preference, along with two additional pulse rate QTL on LG1, revealing a more resolved picture of the genetic architecture underlying mating behavior divergence. Our sequence-based genetic map, along with dramatically narrowed QTL confidence intervals, allowed us to annotate genes within the QTL regions and identify several exciting candidate genes underlying variation in pulse rate and preference divergence. Such knowledge suggests potential molecular mechanisms underlying the evolution of behavioral barriers.

Pheromonal enrichment in the zoo: An empirical approach with Asian elephants (Elephas maximus)

The use of odors is a popular form of sensory enrichment, yet few studies have investigated the longer lasting effects of biologically relevant chemical signals like pheromones. Asian elephants (Elephas maximus) are unique because they are commonly held in zoos and utilize two single-compounds to synchronize reproduction: frontalin (a pheromone produced by male elephants in musth) and (Z)-7-dodecenyl acetate (Z7-12:Ac, a pheromone produced by female elephants around ovulation). While Asian elephant chemical signaling dynamics have been well-studied, no reports exist on the broader behavioral effects (i.e., primer effects) of either of these pheromones. The purpose of this study was to describe the primer effects of both frontalin and Z7-12:Ac for potential enrichment applications. We bioassayed 50 elephants at 10 zoos using synthetic versions of these two pheromones that replicated the concentrations at which they are produced naturally. We found that behavioral diversity in response to these pheromones was influenced both by the degree of exposure to the chemicals and by properties of the receiver, such as sex, sexual experience, age, and social access, with models that included all or most of these factors explaining much of the variation in the bioassay data for frontalin (R2(c) = 0.42) and Z7-12:Ac (R2(c) = 0.59). Furthermore, both males and females exhibited distinct changes in activity levels in the presence of frontalin or Z7-12:Ac, with specific behavioral differences noted. For instance, frontalin stimulated increased activity (and decreased inactivity) among female elephants (P < 0.001). Foraging (P = 0.029) and walking (P = 0.001) increased when males were exposed to frontalin, but it also stimulated increased time spent engaged in stationary stereotypy (P < 0.001). On the other hand, Z7-12:Ac encouraged greater activity levels in female (P < 0.001) and male (P = 0.010) elephants. These results support the use of pheromones as enrichment opportunities for elephants, and suggest appropriate chemical signals should be considered for other species. Because these compounds hold biological, ecological, and/or evolutionary salience, they can have long-lasting effects and are particularly relevant to long-term management strategies. In conjunction with other forms of sensory enrichment—including biologically novel odors—pheromones have the potential to enhance the wellness of zoo-housed species.

Forager age and foraging state, but not cumulative foraging activity, affect biogenic amine receptor gene expression in the honeybee mushroom bodies.

Foraging behavior is crucial for the development of a honeybee colony. Biogenic amines are key mediators of learning and the transition from in-hive tasks to foraging. Foragers vary considerably in their behavior, but whether and how this behavioral diversity depends on biogenic amines is not yet well understood. For example, forager age, cumulative foraging activity or foraging state may all be linked to biogenic amine signaling. Furthermore, expression levels may fluctuate depending on daytime. We tested if these intrinsic and extrinsic factors are linked to biogenic amine signaling by quantifying the expression of octopamine, dopamine and tyramine receptor genes in the mushroom bodies, important tissues for learning and memory. We found that older foragers had a significantly higher expression of Amdop1, Amdop2, AmoctαR1, and AmoctβR1 compared to younger foragers, whereas Amtar1 showed the opposite pattern. Surprisingly, our measures of cumulative foraging activity were not related to the expression of the same receptor genes in the mushroom bodies. Furthermore, we trained foragers to collect sucrose solution at a specific time of day and tested if the foraging state of time-trained foragers affected receptor gene expression. Bees engaged in foraging had a higher expression of Amdop1 and AmoctβR3/4 than inactive foragers. Finally, the expression of Amdop1, Amdop3, AmoctαR1, and Amtar1 also varied with daytime. Our results show that receptor gene expression in forager mushroom bodies is complex and depends on both intrinsic and extrinsic factors.

&lt;i&gt;Damunwha&lt;/i&gt; in South Korea: A Case Study of Divergences in Cognition and Behavior

In South Korea, over 10% of new couples involve a foreign bride. Most come from Southeast Asia (Vietnam, Philippines, Thailand, Cambodia, Indonesia), and others from East Asia (China, Japan). Such couples now produce almost 6% of births. Their children tend to do badly at school and many drop out, the commonly cited reason being the child’s poor acquisition of language skills from a foreign mother. In reality, Damunwha (“multicultural children”) have no trouble with spoken Korean. Their deficiency is in written Korean, particularly in literary and specialized vocabulary that is largely learned at school. They actually do well in subjects that emphasize the spoken language and social interaction, like music, painting, and physical education. They do badly only in those subjects that require abstraction and memorization, like mathematics and social studies. Damunwha children are also more prone to hyperactivity, impulsivity, and non-compliance with rules. These divergences in cognition and behavior seem confined to children of Southeast Asian mothers, since children of Chinese or Japanese mothers perform as well as those of unmixed Korean parentage. It looks as if the country’s social norms, particularly those of Confucianism, favored the spread of certain cognitive and behavioral traits within the Korean population, and more broadly among East Asians. These traits include not only high cognitive ability but also a high capacity to obey rules, to defer gratification, and to control impulsive behavior.

Coupled dynamics of behaviour and disease contagion among antagonistic groups.

Disease transmission and behaviour change are both fundamentally social phenomena. Behaviour change can have profound consequences for disease transmission, and epidemic conditions can favour the more rapid adoption of behavioural innovations. We analyse a simple model of coupled behaviour change and infection in a structured population characterised by homophily and outgroup aversion. Outgroup aversion slows the rate of adoption and can lead to lower rates of adoption in the later-adopting group or even behavioural divergence between groups when outgroup aversion exceeds positive ingroup influence. When disease dynamics are coupled to the behaviour-adoption model, a wide variety of outcomes are possible. Homophily can either increase or decrease the final size of the epidemic depending on its relative strength in the two groups and on R0 for the infection. For example, if the first group is homophilous and the second is not, the second group will have a larger epidemic. Homophily and outgroup aversion can also produce dynamics suggestive of a 'second wave' in the first group that follows the peak of the epidemic in the second group. Our simple model reveals dynamics that are suggestive of the processes currently observed under pandemic conditions in culturally and/or politically polarised populations such as the USA.

Coping and Co-creation: One Attempt and One Route to Well-Being. Part 1. Conceptual Framework

Background. All life strives to be well, but not all life is well. This suggests that cognition aimed at improving and protecting well-being might share a common core across all life forms: core cognition Objective. In this first of a two-part theoretical article, we systematically specify the evolutionary core cognition of well-being from the perspective of general living agents. In Part 2 we apply this to identity development and the theoretical approaches to well-being. This first part aims to identify the strategies and conditions for the creation and protection of generalized well-being and describes associated behavioral ontologies. Results. We defined a set of key terms that, together, specify core cognition. This set comprises quite naturally concepts like agency, behavior, need satisfaction, intelligence, authority, power, and wisdom, which are all derived from the defining properties of life. We derived coping and co-creation as two essentially different, but complementary, behavioral ontologies. Coping is for survival and targeted problem solving and aims to end the need for its activation. Co-creation is for thriving and problem prevention and aims to perpetuate its activation. Co-creation can explain the growth of the biosphere. While both strategies are essential, the successful interplay of their strengths leads to the dominance of one of them: co-creation. Absence of success leads to a dominance of coping: a coping-trap and a strong urge to curtail behavioral diversity. We summarize the key terms of core cognition and the ontologies in two tables with defined terms.

Odonate ethodiversity as a bioindicator of anthropogenic impact

The increasing use of dragonflies and damselflies as models in studies on biodiversity in the last decades has unraveled several features of natural processes and mechanisms for species conservation. Nevertheless, biodiversity is a polysemic concept that resolves multiple dimensions that, together, enroll what we observe as species and lineages diversity. One of these dimensions is Ethodiversity, which may represent the individual diversity of behavioral traits and higher organization levels. Hence, measures of Ethodiversity may be used as indicator tools to measure such dimensions of biodiversity. However, we still lack methods and protocols to measure this diversity. Therefore, here we addressed whether damselfly behaviors may act as indicators of environmental impacts. We collected behavioral data of 120 males in two sites, one in an ecological reserve and another in an impacted habitat. Our results show differences in behavioral syndromes and behavioral integrity when comparing populations in impacted and conserved environments. In conclusion, we hope that these results stimulate future endeavors to create a methodological framework to assess behavioral diversity.