Dog Behavior Research Articles

Comparing standard screening questionnaires of canine behavior for assessment of cognitive dysfunction.

Canine cognitive dysfunction (CCD) is a common, yet underdiagnosed neurodegenerative disease affecting older dogs. Treatment is most effective when started early, so identifying mild cognitive decline in the earlier stages of the disease is considered important. To compare the results of three different standard screening questionnaires [Canine Dementia Scale (CADES), Canine Cognitive Assessment Scale (CCAS), and Canine Cognitive Dysfunction Rating Scale (CCDR)] for CCD diagnosis. Trainability, pain sensitivity, and fear were additionally assessed with the Canine Behavioral Assessment and Research Questionnaire (C-BARQ) in order to evaluate associations between the three dementia scales and behavior. An online survey containing all the mentioned questionnaires was designed for and distributed among owners of elderly dogs. Data from 597 dogs were analyzed. Overall, the scores of the three CCD questionnaires correlated well with each other, especially those of the CADES and CCAS. The CADES was more sensitive in identifying dogs with already mild to moderate cognitive impairment, while the others classified them as still undergoing normal aging. CCD scores increased for all questionnaires with age with spatial orientation being a key feature in CCD development. Trainability assessed with the C-BARQ decreased significantly with severity of CCD signs, while pain sensitivity increased. Fear and anxiety was pronounced in animals with mild but not with severe CCD. These associations based on the C-BARQ were more clearly observable in relation to CADES and CCDR than CCAS. The choice of screening questionnaire impacts the evaluation of cognitive status and severity of CCD. Thresholds for severity classification differ significantly and may have an impact on reliable assessment. Further longitudinal studies are required to determine which of the questionnaires investigated in this study is best suited for early detection of CCD.

Differences in small-breed dogs' body language and vocalizations in a negative context

A dog's emotional state is communicated primarily through body language, readily observable by humans and other animals. Additionally, dogs express visual and auditory signals differently according to the situation they are experiencing. However, there is a need for more research on smaller breeds since they tend to display more behavioral issues than larger breeds. The objective of the present study was to identify the behavioral characteristics of 25 dogs of three different small breeds (Chihuahua, Toy Poodle, and Miniature Schnauzer) in a negative context. We used a web-based tool called the Dog Actions Video Annotation Interface (DAVAI) to evaluate the movements of the tail, legs, snout, and vocalizations when the dogs were exposed to unfamiliar people. Our results showed no significant differences in dog behavior associated with breed. However, we identified variations in dog vocalizations explained by the sex, with male dogs whining more frequently than females. We highlight the importance of understanding dog body language and vocalizations, regardless of their breed. Our study is particularly relevant in negative contexts, where dogs may exhibit distress signals that require prompt intervention to alleviate their suffering.

Puppy whines mediate maternal behavior in domestic dogs

In mammals, offspring vocalizations typically encode information about identity and body condition, allowing parents to limit alloparenting and adjust care. But how do these vocalizations mediate parental behavior in species faced with the problem of rearing not one, but multiple offspring, such as domestic dogs? Comprehensive acoustic analyses of 4,400 whines recorded from 220 Beagle puppies in 40 litters revealed litter and individual (within litter) differences in call acoustic structure. By then playing resynthesized whines to mothers, we showed that they provided more care to their litters, and were more likely to carry the emitting loudspeaker to the nest, in response to whine variants derived from their own puppies than from strangers. Importantly, care provisioning was attenuated by experimentally moving the fundamental frequency (fo, perceived as pitch) of their own puppies' whines outside their litter-specific range. Within most litters, we found a negative relationship between puppies' whine fo and body weight. Consistent with this, playbacks showed that maternal care was stronger in response to high-pitched whine variants simulating relatively small offspring within their own litter's range compared to lower-pitched variants simulating larger offspring. We thus show that maternal care in a litter-rearing species relies on a dual assessment of offspring identity and condition, largely based on level-specific inter- and intra-litter variation in offspring call fo. This dual encoding system highlights how, even in a long-domesticated species, vocalizations reflect selective pressures to meet species-specific needs. Comparative work should now investigate whether similar communication systems have convergently evolved in other litter-rearing species.

Puppy Socialisation Experiences in Relation to Age and COVID-19 Lockdown Restrictions in the UK and ROI.

Limited socialisation can contribute to the development of undesirable dog behaviours. The COVID-19 lockdown potentially limited socialisation opportunities, which may negatively impact the future behaviour of puppies raised during lockdown. Data were gathered from longitudinal study participants in the United Kingdom/Republic of Ireland via multiple questionnaires between May 2016 and November 2022. The impact of age and lockdown phase (pre-, during, and post-) on the types of socialisation experiences of 8-to-19-week-old puppies and the recency of socialisation experiences of approximately 6-month-old puppies were examined. Puppies under 19-weeks had significantly more types of socialisation experiences (from a predefined list) as they aged, and pre-lockdown compared to post-lockdown, but not between other lockdown phases. Most 6-month-old puppies had met a new adult or dog outside the household, a familiar dog, and/or a child within the last 1-7 days, and this was similar between lockdown phases. During lockdown, 6-month-old puppies experienced longer periods between meeting a new adult in their home. Puppies were hypothesised to have had fewer experiences during lockdown, but this was not found. However, the quantity and quality of these experiences may have been affected. Future research within this longitudinal study will explore relationships between the timing and type of experiences had by puppies and their subsequent behaviour.

Effects of Gabapentin on the Treatment of Behavioral Disorders in Dogs: A Retrospective Evaluation.

The use of gabapentin in treating dogs with behavioral disorders is not well described. To characterize behavioral effects of gabapentin, this study surveyed 50 owners whose dogs were prescribed gabapentin at a veterinary behavior-focused practice over a five-year period. Most owners (72%) reported that gabapentin was moderately or very effective at improving their dog's behavior. The majority of owners reported at least one side effect (70%), with sedation being the most common. Sedation was more likely to be seen at doses higher than 30 mg/kg. Specific dose ranges (mg/kg) did not correlate with any other reports of side effects nor effectiveness. Dogs with a diagnosis of conflict-related aggression were more likely to have owners report that gabapentin was effective at improving behavior compared to dogs with other behavioral diagnoses (p = 0.04), while dogs diagnosed with aggression secondary to high arousal were less likely to have owners report that gabapentin was effective (p = 0.01). Overall, reports of effect varied widely and, with the exception of sedation, did not correlate with specific mg/kg dose ranges. Results suggest that some dogs may be more sensitive or resistant to adverse and/or therapeutic effects than others and multiple dosage trials may be needed before finding the best fit.

Beyond barks and bodies: Pup play and body image in gay, bi, trans, and queer men through a Deleuzian lens

Body image is a complex issue that affects individuals from all genders and sexual orientations. Within the queer community pup play is a BDSM-related activity where participants take on the role of a pup/dog, complete with barking, crawling, and other canine behaviors. In this research, we explored how identifying as a pup shapes the body image of gay, bisexual, queer, and trans (GBQT) men using a Deleuzian approach. The process of cellphilming included attendance of three online workshops and the creation of a short cellphilm about their body image. Seventeen participants across five groups completed the workshops and submitted a cellphilm. Data was analyzed through discourse analysis, and while our analysis includes workshop quotes and film excerpts, the true depth of the films, capturing elements like lighting and sound, goes beyond the transcribed words. Three overarching discursive considerations are reported: (i) reinforcing body standards for GBTQ men, (ii) capacity for changing feelings about bodies, and (iii) becoming a pup. Participants suggested that although the pup community is more accepting of diverse bodies, hegemonic body standards for GBTQ men still apply. However, participants also described how identifying as a pup and connecting with others in the pup community was a way to navigate body image tensions.

Assessing the effectiveness of cannabidiol additive supplementation on canine behavior and cortisol levels

In veterinary medicine, Cannabis has been used to treat pain conditions, inflammation, and seizures. However, little is known about its effect on dogs’ behavior. This preliminary research aims to address this knowledge gap by evaluating the effectiveness of cannabidiol (CBD) oil in canine behavioral therapy. Twenty dogs, diagnosed with behavioral disorders and housed in a municipal shelter, participated in a double-blind trial. Ten dogs received CBD oil treatment, while the other ten received a control oil without CBD. Before (T0) and after (T1) the treatment, all the dogs underwent a temperament test to assess their behavior in the presence of four different stimuli: a human stranger, a novel object, a child-like doll, and a conspecific (another dog). Each stimulus was presented individually, and the dogs' behaviors were recorded on video and analyzed. Additionally, hair samples were collected using a shave-reshave technique for cortisol determination through Radio-Immuno-Assay. No behavioral differences were found between the two groups at both T0 and T1. There were no significant differences in the behavioral responses of either group when comparing T0 and T1. However, individual responses to the CBD oil treatment appeared to vary among subjects. A significant increase in hair cortisol levels (p-value <0.05) was observed in the group treated with CBD oil [T0 = 1.60 (1.44–1.93) pg/mg, T1 = 4.81(2.57–6.01) pg/mg]. These findings highlight the importance of individualized treatment when using Cannabis and encourage further research on the use of CBD oil in animal behavioral medicine.

Training Methods Used by Dog Guardians in the United States: Prevalence, Sources of Information, and Reasons for Use.

While there has been recent attention in the scientific community on the ethical and welfare implications of different dog training methods, less research has investigated what methods and training tools United States dog guardians use, where they obtain information about dog training, and the reasons they give for their choices of method. We conducted two surveys with nearly 800 Arizona State University undergraduate students to gain a more realistic look into how dog guardians in the United States train their dogs and where they are receiving their training information. Only 5% of respondents reported utilizing a trainer when they had concerns regarding their dog's behavior; 60% would ask a friend or family member or seek advice online. Few reported taking their dog to any training classes; 70% reported either training the dog themselves or not implementing any formal training. When asked general questions, most respondents reported using rewards-based methods but, when asked about specific problem behaviors, 57% of respondents noted that they would use auditory or physical corrections. Respondents who trained with rewards-based methods reported that these methods of training were more effective significantly more frequently than those who trained with mixed methods reported that those methods were most effective (Fisher's Exact Test, p < 0.01).

Comparison of activity trackers in estimating canine behaviors

Classifying behavior by tracking acceleration has received increased interest lately. Here, we evaluated the performance of three commercial activity trackers in differentiating seven dog behaviors. Adult companion dogs (N = 70) performed still (lying, sitting, standing) and dynamic (walking, sniffing, trotting, playing) tasks, while wearing ActiGraph GT9X Link, Kaunila and FitBark devices placed on the neck collar and ActiGraph GT9X Link placed on the back. Each task was performed for 3 min within a session and repeated in two sessions; the behaviors were confirmed from video recordings. Activity scores of devices were calculated as median values for behavioral differentiation, and as minute-based values for inter-device correlations and cutoff analysis. Measurements of all devices correlated with each other, and median activity scores of all devices − unaffected by dog age, weight or sex − differentiated the still from dynamic behaviors. Dynamic behaviors were also differentiated from each other, with exception of walking vs. sniffing by back-placed ActiGraph GT9X and Kaunila. The definition of cutoffs between behaviors varied from moderate to high accuracy; defined cutoffs for standing and walking were the least accurate. The classification performance of the cutoffs had an accuracy of 80% in all the devices; thus, they performed reasonably well in classifying these behaviors.

Three-Dimensional Cultivation a Valuable Tool for Modelling Canine Mammary Gland Tumour Behaviour In Vitro

Three-Dimensional Cultivation a Valuable Tool for Modelling Canine Mammary Gland Tumour Behaviour In Vitro

Human Perception of Emotions from Canis familiaris Barks: An Auditory-Perceptual Study

This study investigates how experience and gender influence the perception of emotions in dog barks. Drawing from Morton's structural-motivational rule and previous research on mammalian vocalizations, we aimed to discern whether humans, especially those experienced with dogs, can accurately identify emotions such as stranger, anger, lonely, and play in dog barks. Using recordings of Indian Lesser Spitz barks in various contexts, we conducted auditory-perceptual experiments with two groups: experienced listeners (with more than 2- 3 years of pet dog experience) and non-experienced listeners. Participants listened to bark sequences and identified the corresponding emotions. Results revealed that experienced listeners consistently outperformed non- experienced ones in identifying emotions, except for 'play,' where no significant difference was observed. Gender did not significantly affect emotion perception. Interestingly, 'anger' was most accurately identified across both groups, followed by 'stranger,' 'play,' and 'lonely' emotions. Analyzing open-ended responses, we found that acoustic cues such as pitch and inter-bark intervals strongly influenced emotional perception. 'Stranger' barks were described as low-pitched, while 'anger' barks had shorter inter-bark intervals. 'Lonely' barks were characterized by high pitch and longer inter- bark intervals than 'play' barks. These findings suggest that experience plays a crucial role in accurately perceiving emotions in dog barks, aligning with the concept of a common mammalian heritage in emotional communication. Gender differences were negligible in this context. Understanding the acoustic cues underlying emotional expression in dogs enhances our comprehension of canine behavior and has implications for fields like animal welfare and neuroscience. Further research could delve deeper into the mechanisms underlying emotional perception in non-verbal communication across mammalian species.

Attachment-like behavioral expressions to humans in puppies are related to oxytocin and cortisol: A comparative study of Akitas and Labrador Retrievers

This study investigated the relationship between urinary hormone concentrations and attachment-related behaviors in two dog breeds, the Akitas and Labrador Retrievers, to elucidate the hormonal and behavioral mechanisms underlying domestication and interspecies attachment to humans. By measuring cortisol and oxytocin concentrations, and conducting the Strange Situation Test (SST), we aimed to investigate breed differences in endocrine secretions associated with domestication and how these differences influence dog behavior toward humans. Our results showed significant breed differences in urinary cortisol concentrations, with Akitas exhibiting higher levels than Labrador Retrievers. This suggests a breed-specific stress response related to genetic proximity to wolves. However, oxytocin concentrations did not differ significantly, which suggests a complex interplay between factors influencing the domestication process and the formation of attachment behaviors. Behavioral observations during the SST revealed breed-specific patterns, with Labrador Retrievers showing more playful and attachment-like behaviors and Akitas showing more exploratory and passive behaviors. The study found correlations between hormones and behaviors within breeds, particularly in Labrador Retrievers, where oxytocin concentrations were associated with attachment-like behaviors, and cortisol concentrations reflected individual differences in physical activity rather than stress responses to social situations. These findings contribute to the understanding of the evolutionary and adaptive processes underlying the ability of domestic dogs to form close relationships with humans while highlighting the role of hormonal mechanisms in mediating attachment behaviors and the influence of breed-specific genetic backgrounds on these processes.

Validation of the shortened version of the Canine Behavioral Assessment and Research Questionnaire (C-BARQ) using participants from the Dog Aging Project.

The Canine Behavioral Assessment and Research Questionnaire (C-BARQ) is a 100-item owner-completed survey instrument used for assessing behavior and temperament of companion dogs. The shortened version of the C-BARQ (C-BARQ(S)) consists of 42 items of the long C-BARQ. We aimed to validate the shortened C-BARQ(S) by comparing it with the long questionnaire in the same human-dog pair. We examined data from a nationwide cohort of companion dogs enrolled in the large-scale longitudinal Dog Aging Project (DAP) study. Among 435 participating owners who completed both the long and shortened versions of the C-BARQ within 60 days of each other, agreement between individual questions of the long and shortened C-BARQ using an unweighted kappa statistic and percent agreement was examined. Associations between the two questionnaires for mean behavior and temperament domain scores and mean miscellaneous category scores were assessed using Pearson correlation coefficients. Of 435 dogs in the study, the mean (SD) age was 7.3 (4.3) years and 216 (50%) were female. Kappa values between the long and shortened C-BARQ for individual questions within the 14 behavior and temperament domains and a miscellaneous category ranged from fair to moderate (0.23 to 0.40 for 21 items and 0.41 to 0.58 for 26 items, respectively). Pearson correlation coefficients above 0.60 between both questionnaires for 12 of the 14 mean behavior and temperament domain scores and a category of miscellaneous items were observed. Kappa values for individual questions between the long and shortened C-BARQ ranged from fair to moderate and correlations between mean domain scores ranged from moderate to strong.

A Method for Assessing Dogs in a Test Evaluating Dogs' Suitability for Animal-Assisted Education.

In objective behavioral rating (BR), dogs are rated for behavior based on the observations made during a behavioral test. However, behavioral definitions can differ between raters, and the definitions proposed by artificial intelligence (AI) can help standardize the applied measures. The aim of this study was to propose a new approach for assessing dogs' suitability for animal-assisted education (AAE) with the use of the BR method and the definitions of dog behaviors proposed by AI. The analysis relied on video footage of 25 dogs that participated in tests assessing the animals' suitability for AAE. The dogs were rated by five independent observers. Inter-rater reliability was satisfactory in 7 out of 9 subtests (0.715-0.856) and low in the remaining 2 subtests (0.667 in Subtest 2 and 0.617 in Subtest 5). The results indicate that the BR method based on AI definitions produced satisfactory inter-rater reliability in a test evaluating dog behavior. A comparison of subtest scores in the BR method and the subjective rating method did not reveal significant differences. Thus, it can be concluded that the proposed approach gives promising outcomes and can be used to develop new tests for assessing dogs' suitability for AAE and, perhaps, other types of work.

Supporting animals and veterans through enrichment: An exploratory study on a brief canine socializing intervention

Abstract Veterans pursuing post-secondary education may experience reintegration challenges and academic-related stress. To support student well-being, colleges and universities have begun to offer animal-assisted interventions (AAI); however, the influence of AAI programs on student veteran populations remains underexplored. Furthermore, the welfare impact of AAI programs on incorporating animals has begun to be assessed. As shelter dogs can benefit from human interaction, a mixed-methods design was used to assess if dogs and student veterans could benefit from participating in a brief 30-min unstructured interaction. We aimed to identify whether: the interaction influenced veteran reported mood state, the dog and the interaction activities completed influenced veteran experience, and the interaction influenced shelter dog behavior. Thirty veterans ( M age = 34, SD = 9.2; 24 males, 4 females) enrolled in a southern United States mid-size public university participated along with screened shelter dogs ( N = 30, M age = 2, SD = 0.9; 17 males, 13 females). Veterans were instructed to engage in the activities of their choosing (i.e., petting, playing, talking, providing treats, brushing) with a shelter dog. To assess changes in veteran mood, a pictorial self-report scale (assessing arousal (activated, deactivated), and valence (pleasant, unpleasant)) was completed pre- and post-interaction. Dog behavior was measured in shelter before the interaction and during the beginning, middle, and end of the interaction. Results reveal veterans reported pleasant post-interaction mood states, an overall positive experience and activity enjoyment. Qualitative results indicated that veterans perceived the dog behavior to be the most memorable aspect of participation, positively influencing their overall interaction experience. Dogs entered the interaction with more behavioral displays of fear (e.g., reduced posture), general activity (e.g., jumping), and soliciting contact behaviors (e.g., soliciting play), with all behavioral activity reducing halfway into the interaction, potentially suggesting increased relaxation over the course of the interaction. Observed decreases in dog fear behavior and veteran reported activity enjoyment suggests benefits for shelter dogs and student veterans.

Two Separate Brain Networks for Predicting Trainability and Tracking Training-Related Plasticity in Working Dogs.

Functional brain connectivity based on resting-state functional magnetic resonance imaging (fMRI) has been shown to be correlated with human personality and behavior. In this study, we sought to know whether capabilities and traits in dogs can be predicted from their resting-state connectivity, as in humans. We trained awake dogs to keep their head still inside a 3T MRI scanner while resting-state fMRI data was acquired. Canine behavior was characterized by an integrated behavioral score capturing their hunting, retrieving, and environmental soundness. Functional scans and behavioral measures were acquired at three different time points across detector dog training. The first time point (TP1) was prior to the dogs entering formal working detector dog training. The second time point (TP2) was soon after formal detector dog training. The third time point (TP3) was three months' post detector dog training while the dogs were engaged in a program of maintenance training for detection work. We hypothesized that the correlation between resting-state FC in the dog brain and behavior measures would significantly change during their detection training process (from TP1 to TP2) and would maintain for the subsequent several months of detection work (from TP2 to TP3). To further study the resting-state FC features that can predict the success of training, dogs at TP1 were divided into a successful group and a non-successful group. We observed a core brain network which showed relatively stable (with respect to time) patterns of interaction that were significantly stronger in successful detector dogs compared to failures and whose connectivity strength at the first time point predicted whether a given dog was eventually successful in becoming a detector dog. A second ontologically based flexible peripheral network was observed whose changes in connectivity strength with detection training tracked corresponding changes in behavior over the training program. Comparing dog and human brains, the functional connectivity between the brain stem and the frontal cortex in dogs corresponded to that between the locus coeruleus and left middle frontal gyrus in humans, suggestive of a shared mechanism for learning and retrieval of odors. Overall, the findings point toward the influence of phylogeny and ontogeny in dogs producing two dissociable functional neural networks.

Pharmacological treatment of canine and feline undesirable behaviors by Finnish veterinarians

Pharmacological treatment of canine and feline undesirable behaviors by Finnish veterinarians

Size matters: Natural experiments suggest the dear enemy effect is moderated by pack size in African wild dogs.

Remote monitoring of communal marking sites, or latrines, provides a unique opportunity to observe undisturbed scent marking behaviour of African wild dogs (Lycaon pictus). We used remote camera trap observations in a natural experiment to test behavioural scent mark responses to rivals (either familiar neighbours or unfamiliar strangers), to determine whether wild dogs exhibit the "dear enemy" or "nasty neighbour" response. Given that larger groups of wild dogs represent a greater threat to smaller groups, including for established residents, we predicted that the overarching categories "dear enemy" vs. "nasty neighbour" may be confounded by varying social statuses that exists between individual dyads interacting. Using the number of overmarks as a metric, results revealed an interaction between sender and receiver group size irrespective of familiarity consistent with this prediction: in general, individuals from large resident packs overmarked large groups more than they overmarked smaller groups, whereas individuals from smaller packs avoided overmarking larger groups, possibly to avoid detection. Monitoring a natural system highlights variables such as pack size that may be either overlooked or controlled during scent presentation experiments, influencing our ability to gain insights into the factors determining territorial responses to rivals.

Evaluation of current practices for dogs engaged in assistance and therapy support programs within Australia

Evaluation of current practices for dogs engaged in assistance and therapy support programs within Australia

Investigating individual learning behaviour of dogs during a yes/no detection task

Detection dogs are frequently tested for their ability to detect a variety of targets. It is crucial to comprehend the processes for odour learning and the consequences of training on an expanding set of target scents on performance. To properly evaluate their ability to identify the target, the only true measure is the dogs' initial response to novel sources, since this excludes learning effects. In this study, we evaluated the individual learning processes of three detection dogs that were pre-trained to differentially respond to a faecal sample of a mare in oestrus (S+) and a faecal sample of the same mare in di-oestrus (S-). After reaching criterion during a test with known training samples, the dogs were tested for generalization to a novel source. Average responses to S+ and S- were calculated as a function of presentation sequence, and Signal Detection Theory was used to further analyse characteristic differences in learning. The results of this study suggest that the ability of individual scent detection dogs to learn within an olfactory discrimination test varies considerably. The information obtained in this study could be helpful for mitigation training. We show that through careful monitoring of individual learning processes, the strategy each dog followed becomes apparent: especially the observations on the dogs' responses to first encounters with novel sample sources. This provides us with more detailed information than the more traditional sensitivity and specificity measures and allows us to better predict the dog's capabilities.