Behavior Of Dairy Cows Research Articles

Effects of prepartum dietary protein level and feed intake on postpartum lactation performance and feeding behavior of multiparous Holstein dairy cows

An experiment was conducted to determine the effects of low and high metabolizable protein (MP) diets when fed for ad libitum and controlled intake during the prepartum period on postpartum lactation performance and feeding behavior of dairy cows. Thirty-six multiparous Holstein cows were blocked by parity, expected calving date, and previous lactation milk yield at -21 d relative to expected calving and were randomly assigned to 1 of 4 close-up period dietary treatments providing low MP (LMP) or high MP (HMP) diets with controlled intake (CNI) or ad libitum intake (ALI). The concentrations of MP were 65 and 90 g/kg dry matter for LMP and HMP diets, respectively, whereas intake was controlled to supply 100 and 160% of the NRC (2001) energy requirements for CNI and ALI groups, respectively. The concentration of net energy for lactation (NEL) in the treatment diets was 1.50 Mcal/kg. All cows were fed a similar lactation diet after calving (1.50 Mcal/kg of NEL and 83.3 g/kg of MP). The HMP diet increased dry matter intake during the first 3 wk and tended to increase dry matter intake over the 9 wk of lactation. Meal size and eating rate increased in the ALI cows during the prepartum period. Meal frequency increased with the HMP diet during the postpartum period. Milk yield increased by 15.2% with the HMP diet over the 9 wk of lactation. The HMP diet increased energy-corrected milk (ECM) yield in CNI versus ALI cows, whereas the LMP diet increased ECM yield in ALI versus CNI cows over the 9 wk of lactation. The increase in ECM yield of LMP-ALI versus LMP-CNI cows was supported by greater body condition loss and serum β-hydroxybutyrate over the 9 wk of lactation. Taken together, these data indicate that prepartum controlled intake of a high protein diet can provide the benefits of both strategies.

Effects of substituting sweet potato flour for ground corn on performance, feeding behavior, and metabolism of dairy cows.

The objective of this study was to evaluate the effect of substituting sweet potato flour for ground corn in rations fed to lactating dairy on milk yield and composition, blood metabolites, and feeding behavior. Twenty lactating Holstein cows from 30 to 60days postpartum were randomly assigned to one of two groups (n = 10 each) and used in a cross-over design trial with two treatments: a standard concentrate with ground corn as an energy source or experimental concentrate with sweet potato flour (SPF) replacing all of the ground corn. Each of the 35-day periods consisted of 14days for adaptation to diet and 21days for data and sample collection. Milk yield, dry matter intake (DMI), and feeding behavior were evaluated daily throughout the trial. Milk samples were collected weekly and blood samples were collected every 3days. Milk was analyzed for fat, protein, lactose, and total solid constituents. Blood was analyzed for glucose, non-esterified fatty acid (NEFA), gamma-glutamyl transferase (GGT), aspartate aminotransferase (AST), total protein (TP), albumin, and urea concentrations. Milk yield (P = 0.62) and composition (fat: P = 0.71; protein: P = 0.12; lactose: P = 0.82; total solids: P = 0.56) were not affected by dietary treatments. There were no differences between treatments in DMI or meal frequency, but total eating time (P = 0.001), feeding time (P = 0.001), and meal duration (P = 0.001) was higher for control compared with SPF. However, feeding rate (P = 0.001) and serum urea concentration (P = 0.001) were higher for SPF compared with control. No differences were observed in serum metabolites and enzymes measured among treatments. The results of this trial indicate that SPF can be substituted for ground corn without impairing the performance, feeding behavior, and metabolism in dairy cows.

Sensor and Video: Two Complementary Approaches for Evaluation of Dairy Cow Behavior after Calving Sensor Attachment.

Simple SummaryWe analyzed whether attaching sensors to the tail of cows for the early detection of calving leads to behavioral changes. In this study, we combined conventional video analysis with data from digital sensor technologies. To detect the potential agitation of cows after calving sensor attachment, we analyzed cow activity behavior. Based on the combined results of video and sensor analysis, there was no clear evidence that attaching sensors to the tail generally altered the ethological pattern of all cows analyzed. However, the investigation of individual cows showed an increase in the frequency of tail raising and rubbing the tail after calving sensor attachment. These changes would be worth analyzing in more detail on a larger scale.Studies evaluating calving sensors provided evidence that attaching the sensor to the tail may lead to changes in the cows’ behavior. Two different calving sensors were attached to 18 cows, all of which were equipped with a rumen bolus to record their activity. Two methodological approaches were applied to detect potential behavioral changes: analysis of homogeneity of variance in cow activity (5 days pre-sensor and 24 h post-sensor) and analysis of video-recorded behavior (12 h pre- and post-sensor, respectively) in a subgroup. The average results across the sample showed no significant changes in the variability of activity and no statistically significant mean differences in most visually analyzed behaviors, namely walking, eating, drinking, social interaction, tail raising, rubbing the tail, and the number of standing and lying bouts after calving sensor attachment. In addition to considering mean values across all cows, individual cow investigations revealed an increased number of time slots showing a significant increase in the variability of activity and an increased frequency of tail raising and rubbing the tail on objects after calving sensor attachment in some cows, which should be investigated in more detail on a larger scale.

A Comparison of Rice Husks and Peanut Shells as Bedding Materials on Dairy Cows' Preferences, Behaviour, and Health.

Simple SummaryGood bedding materials can increase the comfort potential of the lying surface and enhance the welfare of cows in intensive dairy farms. The preference, behaviour, hygiene, and health of cows are affected by different bedding surfaces. In the current study, we evaluated the preference, behaviour, cleanliness, and health of cows on three bedding materials, peanut shells (PS), rice husks (RH), and a combination of two-thirds peanut shells, one-third rice husk (PRC). The daily behaviour, serum metabolites, and productivity of dairy cows were all within normal values, and no statistical differences were found between all three bedding materials, although cows showed a preference for rice husk when given access to all three bedding materials at the same time. Finally, the results suggest that bedding comprised of peanut shells and peanut–rice combinations are all suitable for maintaining the health and comfort of dairy cows.The provision and quality of bedding materials affect the behaviour, welfare, and health of dairy cows. The objective of this study was to evaluate the preference, behaviour, cleanliness, and physiological status of cows on three bedding materials, peanut shells, rice husks, and a combination of two-thirds peanut shells, one-third rice husk. In an initial experiment, 15 nonlactating, pregnant Holstein cows had free access to all 3 bedding treatments for 39 d. Cows spent more time lying down on rice husk (337 min/d) than on peanut–rice combination (212 min/d) and peanut shell (196 min/d) (p < 0.05), and lay down most often on rice husk (4.35 bouts/d) than on peanut shell (2.55 bouts/d) (p < 0.05) but did not differ between peanut shells and peanut–rice combinations in terms of lying time and lying bouts. In Experiment 2, 12 nonlactating cows were used to assess the effects of the 3 bedding materials on dairy cow behaviour, cleanliness, serum indicators, and productivity. The total duration of lying down (PS: 699.1 min/d, PRC: 645.6 min/d, RH: 852.5 min/d), the frequency of bouts of lying down (PS: 8.7 bouts/d, PRC: 7.6 bouts/d, RH: 11.1 bouts/d), and the mean duration of lying bouts (PS: 83.5 min/bouts, PRC: 91.8 min/bouts, RH: 81.4 min/bouts) did not differ between treatments. Similarly, no differences in eating or drinking behaviour of dairy cows were observed. In terms of hygiene, cleanliness scores did not differ between the three bedding materials, but udder and flank cleanliness decreased and improved, respectively. In addition, treatments did not affect serum metabolites or productivity of the cows. In summary, daily behaviour, serum metabolites, and productivity of dairy cows were all within the normal range, and no statistical differences occurred between the three bedding materials, although cows showed a preference for rice husk when given access to all three bedding materials at the same time. Finally, the results suggest that bedding comprised of peanut shells and peanut–rice combinations are all suitable for maintaining the health and comfort of dairy cows.

Validation of a real-time location system for zone assignment and neighbor detection in dairy cow groups

Tracking data are increasingly used for studying social behavior in dairy cows but guidelines are not available regarding the appropriate system setup and data quality. In this study, we investigated the effects of system calibration, as well as data filtering and smoothing methods on the detection of the location and neighbor preferences of dairy cows in a barn using an ultra-wideband real-time location system (RTLS). We followed a group of 15 or 14 cows during two periods: period 1 with RTLS calibration using a laser distance meter; and period 2 with RTLS calibration using professional surveying technology. During both periods, tracking data were collected every 1.73 s for 3 days for all cows in the group together with data from an electronic feeder system. We performed continuous video analysis of cow locations for 1 day in both periods. We compared the effects of four different filtering and smoothing methods (i.e., Kalman filter, sliding window, jump filter, and median filter) on data preparation using raw tracking data. We assigned specific areas (i.e., lying stalls, walking alley, brush area, feed, and water bins) to the measured X-Y coordinates and compared them with the video-based zone assignments. Sensitivity and precision were calculated to evaluate the quality of the zone assignments corresponding to the different RTLS calibration setups and the effects of filtering and smoothing methods. When the RTLS was accurately calibrated these methods did not result in further improvements. The zone assignments agreed well for the video and all of the prepared tracking data at the lying stalls in both periods, and the agreement was good during period 2 at the feed bunk (sensitivity and precision >0.85). When using zone-based and distance-based approaches for detecting neighbors at the lying stalls and feed bunk based on the prepared tracking data we found high correlations (>0.9) with the neighbor information based on video and electronic bin data in period 2. However, the zone-based approach resulted in the lowest mean absolute error. The quality of the tracking data appeared to be especially important when detecting cows in small areas and with short visit durations (e.g., feed bunk). Our results highlight the importance of accurate RTLS calibration setup and the quality of tracking data when inferring dairy cow behavior based on it.

Effect of dietary non-fiber carbohydrate source and inclusion of buffering on lactation performance, feeding behavior and milk stability of dairy cows

The present study hypothesized that partial replacement of ground corn (GC) by pelleted citrus pulp (PCP) and buffering (BF) inclusion in diets of lactating cows does not change lactation performance, but does reduce milk concentration of ionic calcium (iCa), increasing its stability at ethanol test and heating at 140 °C (heat coagulation time – HCT). We evaluated the effect of PCP and buffer inclusion on digestive metabolism, feeding behavior, milk yield, and milk stability of lactating cows. Sixteen Holstein cows (average milk yield of 28 ± 4.8 L/d and days in milk of 130 ± 81.0; mean ± SD) were distributed in a Latin square design with 4 replicated squares, 4 periods of 21 d (14 d for adaptation and 7 d for sampling) and 4 treatments in a 2 × 2 factorial arrangement. Treatment factor 1 was the partial replacement (134 g/kg) of GC by PCP and factor 2 was the inclusion of buffer (7.1 g/kg of Na bicarbonate plus 2.4 g/kg of Mg oxide; DM basis) in diets composed of 40:60 of forage: concentrate ratio. The partial replacement of GC with PCP reduced dry matter intake (DMI) by 1.35 kg/d, increased dry matter (DM) and neutral detergent fiber assayed with a heat stable amylase and expressed exclusive of residual ash (aNDFom) digestibility, and ruminal concentration of acetic and butyric acids, but no change was observed on milk yield, resulting in higher production efficiency. The partial replacement of GC with PCP also reduced the ruminal concentration of iso-butyric and iso-valeric acids, milk urea nitrogen concentration, and casein content and tended to reduce HCT. There was no isolated effect of GC replacement or buffer addition or their interaction on iCa concentration and milk stability at the ethanol test. For diets without the inclusion of PCP, cows increased drinking time by 8 min/d. When cows were fed diet with the inclusion of PCP, the rumination time decreased 57 min/kg physical effective aNDFom (peNDF) intake compared to when cows were fed diets without partial replacement of GC with PCP. The buffer inclusion increased milk fat content and urinary pH. However, milk stability at ethanol test did not change in response to treatments and HCT tended to be reduced when GC was replaced by PCP. The results of our study suggested that, although partial replacement of GC with PCP and BF inclusion altered DMI, short chain fatty acids (SCFA) concentration, and urinary pH, those factors did not change milk yield and milk stability.

The lying and rumination behavior of healthy dairy cows housed in tie stalls is associated with bedding depth

The lying and rumination behavior of dairy cows is internationally used as an animal welfare indicator. Dairy cow housing in Switzerland is subordinated to detailed legal requirements. It is particularly described that the lying area for cows must be covered with a sufficient amount of bedding. In the current study, we investigated whether bedding depth influences the lying and rumination behavior and thus, the animal welfare of healthy dairy cows housed in tie stalls. Bedding depth was categorized as ≤ 2 cm and > 2 cm. The lying and rumination behavior of 145 cows from 33 farms was recorded and analyzed considering farm effects; 105 cows were housed on ≤ 2 cm bedding, and 40 cows on > 2 cm bedding. Cows on > 2 cm bedding had on average a one hour longer daily lying time compared with cows on ≤ 2 cm bedding (mixed-effects linear regression: mean difference [cows on > 2 cm bedding- cows on ≤ 2 cm bedding] = 60,7 min/d; standard error = 28,6 min/d; p-value = 0,037). The odds of a cow to lie while ruminating were significantly higher in cows on > 2 cm bedding against cows on ≤ 2 cm bedding (mixed-effects logistic regression: Odds ratio = 1,27; CI95% [1,17-1,38]; p-value 2 cm can make a significant difference in the lying and rumination behavior of healthy dairy cows housed in tie stalls. Consequently, the animal welfare of dairy cows in tie stalls is improved by providing at least 2 cm of bedding.

Partial replacement of corn silage with whole-plant soybean and black oat silages for dairy cows

This study aimed to evaluate the effects of partially replacing corn silage (CS) with whole-plant soybean silage (SS) or black oat silage (OS) on nutrient intake and digestibility, in vitro neutral detergent fiber degradability of silages, feeding behavior, rumen fermentation, and performance of dairy cows. Twenty-four lactating Holstein cows (6 of which were rumen-cannulated) with 32.5 ± 4.92 kg/d milk yield, 150 ± 84.8 days in milk, and 644 ± 79.0 kg of body weight were used in a 3 × 3 Latin square design to evaluate the following treatments: (1) corn silage diet (CSD): using corn silage as the only forage source in the diet [48% dietary dry matter (DM)]; (2) whole-plant soybean silage diet (SSD): SS replacing 16% of corn silage from CSD; and (3) black oat silage diet (OSD): OS replacing 16% of corn silage from CSD. The inclusion of OS and SS decreased intakes of DM, organic matter, and crude protein. Corn silage had the greatest in vivo effective degradability of DM, and SS had the least effective degradability of neutral detergent fiber. The OSD treatment decreased milk and protein yields, whereas SSD increased rumen ammonia nitrogen concentration compared with the other diets. Cows fed OSD exhibited a greater preference for feed with small particles (<4 mm) compared with those fed SSD. Cows fed treatments containing either SS or OS at the expense of CS had increased rumination and chewing activities. Although replacing CS with OS and SS reduced feed intake, SS had no effect on productive performance of dairy cows.

Estrus expression of dairy cows after calving with and without using cooling system

This study aimed to compare the estrus expression of postpartum Friesian Holstein (FH) dairy cows with and without the cooling system in Cendana, Enrekang. A total of 9 postpartum FH dairy cows were used in this study, 3 of them were placed in the cold house and the other 6 cows were placed without a cooling system. The feeding consisted of forage, rice bran, and water, which was free access; ad libitum. The parameters measured in this study were (1) the signs of secondary estrus signs, such as vulva condition, mating behavior, mucus secretions, and (2) the duration of estrus, which was counting from the first estrus to the last observation where the cows were no longer showing the estrus symptoms. These parameters were compared by using the t-test. The results showed that the cervical mucus of dairy cows with the cold house was clearer intensity than those without a cooling system (66.7 vs. 16.7%). The changing behavior of dairy cows with cold houses showed higher intensity than without cold houses (100 vs. 67%). The duration of estrus in dairy cows with cooling had significantly (P<0.05) longer than those without cooling (24.4±1.5 vs. 18.3±2.2 hours). It can be concluded that the signs of secondary estrus for those dairy cows in the cooling system showed a clear appearance than those dairy cows without a cooling system. The duration of estrus with a cooling system was longer than without a cooling system.

Forage particle size: it s implications on behavior, performance, health and welfare of dairy cows

Forage particle size: it s implications on behavior, performance, health and welfare of dairy cows

Identification of discriminating behavioural and movement variables in lameness scores of dairy cows at pasture from accelerometer and GPS sensors using a Partial Least Squares Discriminant Analysis

The behaviour and movement of lame dairy cows at pasture have been studied little, yet they could be relevant to improve the automatic detection of lameness in cows in pasture-based systems. Our aim in this study is to identify behavioural and movement variables of dairy cows at pasture that could discriminate lameness scores. Individual cow behaviours were predicted from accelerometer data and movements measured using GPS data. Sixty-eight dairy cows from three pasture-based commercial farms were equipped with a 3-D accelerometer and a GPS sensor fixed on a neck collar for 1–5 weeks, depending on the farm, in spring and summer 2018. A lameness score was assigned to each cow by a trained observer twice a week. Behaviours were predicted every 10 s based on accelerometer data, and then combined with the GPS position. Segmentation on behavioural time series was used to delineate each behavioural bout within each outdoor period. Thirty-seven behavioural and movement variables were then calculated from the behavioural bouts for each cow. A partial least square discriminant analysis was performed to identify the variables that best discriminate lameness scores. Time spent grazing, grazing bout duration, duration before lying down in the pasture, time spent resting, number of resting bouts, distance travelled during grazing, and dispersion were the most discriminant variables in the PLS-DA (VIP > 1). Severely lame cows spent 4.5 times less time grazing and almost twice as much time resting as their sound congeners, especially in the lying position. Exploratory behaviour was also reduced for both moderately and severely lame cows, resulting in 1.2 and 1.7 times less distance travelled respectively, especially during grazing. These variables could be used as additional variables to improve the performance of existing lameness detection devices in pasture-based systems.

The use of an impeller mowing conditioner during haymaking had no effects on feeding behavior, feed intake or performance of organic dairy cows

AbstractImpeller mowing conditioners are commonly used to speed up the drying process on the field, making forage preservation (haying, ensiling) less dependent on weather conditions. However, the effects of this technique on the nutritive value of the forage and dairy cows' responses have not been investigated yet. Each half of two fields of grass-dominated swards, first regrowth, was cut either with or without the use of an impeller mowing conditioner (experimental hay and control hay, respectively).Ceteris paribusconditions were guaranteed by the same cutting and wilting times (roughly 48 h), number of teddings, field pickup technique and barn-drying method. At the beginning of the feeding trial, 19 lactating Holstein cows were allocated to one of two groups, one control (nine cows) and one experimental group (10 cows) and were fed the respective forage plus a fixed amount of concentrate [3.6 kg d−1; dry matter (DM) basis]. After a 14-d adaptation period, data were collected over 21 consecutive days. Covariate data of cows were collected prior to the experimental feeding period, over a time span of 9 d, and included in the statistical model. Results revealed that control and experimental hay showed significant (P&lt; 0.05) differences in the nutrient profile. However, the magnitude of these differences was not enough to affect intakes of hay (18.4 ± 0.29 kg DM d−1), total dietary energy or chewing activity, but did lead to a decreased intake of water-soluble carbohydrates and an increased crude protein intake, thus affecting ruminal nitrogen balance (P&lt; 0.01). This resulted in a higher milk urea content [23.3vs17.9 mg (100 mL)−1;P&lt; 0.01] in cows fed the experimental hay, whereas other milk performance parameters remained unaffected. In conclusion, the use of the impeller mowing conditioner did not affect the overall forage utilization by cows when the diet contained about 16% concentrate (DM basis). As this is the first study dealing with the effects of an impeller mowing conditioner on cows' responses, future research should consider investigating the effects of mowing conditioners when cows are fed only forage or diets with lower concentrate amounts.

A Preliminary Investigation of Social Network Analysis Applied to Dairy Cow Behavior in Automatic Milking System Environments.

Simple SummaryCows are social animals, therefore understanding the ways that they interact can help improve their management and welfare. We used social network analysis (SNA) to data on voluntary cow movement through a sort gate in an automatic milking system to identify pairs of cows that repeatedly passed through a sort gate in close succession (affinity pairs). Results from this exploratory study showed that when cows were separated from their affinity-pair cow the day-day variability in milk production increased by a factor of 3, a possible indicator of stress. The results of this exploratory study suggest that SNA could be used as a tool to better understand the social dynamics of dairy cows and inform group and regrouping process to produce positive outcomes.We have applied social network analysis (SNA) to data on voluntary cow movement through a sort gate in an automatic milking system to identify pairs of cows that repeatedly passed through a sort gate in close succession (affinity pairs). The SNA was applied to social groups defined by four pens on a dairy farm, each served by an automatic milking system (AMS). Each pen was equipped with an automatic sorting gate that identified when cows voluntarily moved from the resting area to either milking or feeding areas. The aim of this study was two-fold: to determine if SNA could identify affinity pairs and to determine if milk production was affected when affinity pairs where broken. Cow traffic and milking performance data from a commercial guided-flow AMS dairy farm were used. Average number of milked cows was 214 ± 34, distributed in four AMS over 1 year. The SNA was able to identify clear affinity pairs and showed when these pairings were formed and broken as cows entered and left the social group (pen). The trend in all four pens was toward higher-than-expected milk production during periods of affinity. Moreover, we found that when affinities were broken (separation of cow pairs) the day-to-day variability in milk production was three times higher than for cows in an affinity pair. The results of this exploratory study suggest that SNA could be potentially used as a tool to reduce milk yield variation and better understand the social dynamics of dairy cows supporting management and welfare decisions.

Spatio-Thermal Variability and Behaviour as Bio-Thermal Indicators of Heat Stress in Dairy Cows in a Compost Barn: A Case Study.

Simple SummaryThe thermal distribution inside a compost-bedded pack barn and the behavioural aspects plays an important role in terms of welfare and sustainability for dairy cows. Through a spatial variability assessment of thermal conditions in a compost barn, we found different regions with comfortable or stressful conditions based on air and bed temperature, as well as wind speed. Regarding the behaviour of cows with different number of lactations, we observed a higher probability of water intake in primiparous cows and increased walking behaviour in multiparous cows during the hottest periods. We suggest that special attention must be given to environmental control in a compost barn, mainly during hot seasons, to avoid different hot spots inside the facility. Additionally, with unbalanced environmental resources, the hierarchy of multiparous over primiparous cows might predominate the alleviation of the herd’s thermal stress.The spatial variability and behavioural aspects of compost-bedded pack barns remain unknown in subtropical regions. In this study, we investigated whether spatial variability occurs in the thermal environment of a compost barn and how the behaviour of dairy cows with different numbers of lactations differs in this system. The spatial sampling design comprised a grid of 108 locations inside the facility. At each location, we measured air temperature, relative humidity, wind speed, and bed temperature at 9:00, 12:00, and 15:00. We performed 24-h behavioural observations. Regarding spatial variability, the north face showed high air temperature values, and the distribution of relative humidity varied from the north to the south face. Kriging maps revealed a high bedding temperature trend, indicating heterogeneous ventilation management. Primiparous cows visited the water trough during the hottest hours of the day, whereas multiparous cows displayed a higher probability of walking during these periods. In conclusion, we observed a heterogeneous management of ventilation through the spatial distribution of the thermal environment inside the compost-bedded pack barn, with multiparous cows exhibiting dominance over primiparous cows seeking environmental resources.

Administration of ketoprofen affects post-partum lying behaviours of Holstein dairy cows regardless of whether parturition is assisted.

Assistance at parturition in cattle is common. Although analgesia is commonly provided, its value in terms of welfare improvement is not known. Few previous studies include both parturition assistance and analgesic treatment status as well as appropriate control groups in a factorial design. Seventy-two Holstein cattle (37 assisted and 35 unassisted) were randomly allocated to receive a single dose of the NSAID ketoprofen or a saline placebo within 3 h of parturition. Detailed behavioural observations allowed a time budget to be constructed for each cow for 48 h post-partum. Cows experiencing assisted parturition spent more time in lateral recumbency (overall and with the head rested) (p < 0.05) - an infrequently adopted posture suggestive of illness or pain. Cows treated with ketoprofen spent less time in lateral recumbency (p < 0.05) (overall and with the head rested); this was independent of assistance status, and lateral recumbency was not affected by the interaction between assistance and treatment status. Additionally, cows treated with ketoprofen spent more time with the head rested when in sternal recumbency (p = 0.009) - a behaviour associated with comfortable resting. These differences in lying postures exhibited by cows receiving ketoprofen analgesia suggest that, regardless of whether parturition is assisted, a single dose of ketoprofen in the immediate post-partum period has the potential to improve cow comfort in the first 48 h post-partum.

Rumen function and grazing behavior of early-lactation dairy cows supplemented with fodder beet

Fodder beet (FB) is a source of readily fermentable carbohydrate that can mitigate early spring herbage deficits and correct the negative energy balance experienced during early lactation in pastoral dairy systems of New Zealand. However, the low-fiber and high-soluble carbohydrate content of both FB bulb and spring herbage are factors that promote subacute ruminal acidosis, impairing rumen function and limiting the marginal milk production response to supplement. In a crossover experiment, 8 Holstein Friesian × Jersey early-lactation dairy cows were used to test the effect of supplementing 16 kg of dry matter (DM) of a grazed perennial ryegrass herbage with 6 kg of DM/d of FB bulb (FBH) versus herbage only (HO) on changes in rumen function and grazing behavior. Following 20 d of adaptation to diets, DM disappearance (%) of FB bulb (FBH cows only) and herbage were measured in sacco, separately. Cows were fasted overnight, and the ruminal contents were bailed the following morning (~0930 h) again to determine the pool size of volatile fatty acids, ammonia, and particle size of digesta, as well as to estimate the rate of ruminal outflow and degradation of neutral detergent fiber. The FBH diet did not alter DM intake, milk yield, or milk solid (fat + protein) production compared with HO. Supplementation of herbage with FB reduced ruminal pH compared with HO between ~0800 h and 1300 h each day. During each period, 1 cow experienced severe subacute ruminal acidosis (pH <5.6 for >180 min/d) during final adaptation to the target FB allocation. The FBH diet reduced the ruminal pool of acetate and ammonia, but increased the ruminal pool of butyrate and lactate compared with HO. When fed FB, rumination and grazing time increased and grazing intensity declined compared with cows fed HO. Despite increased rumination, the comminution of large particles declined 28% between the first and second rumen bailing when cows were fed FB, and in sacco DM disappearance of perennial ryegrass declined 18% compared with cows fed HO. These results indicate that grazing dairy cows supplemented with FB (40% of daily intake) increase rumination and mastication intensity to counteract reduced ruminal degradation of ryegrass herbage due to low ruminal fluid pH.

Possible causes of aberrations in adverse grouping behavior of dairy cows: A field study

In the Dutch national surveillance system, an increasing number of reports were received in the summer of 2017 from farmers about unusual behavior of their cows. The cows were grouping during the day in summer in one part of the barn and did not move for several hours, which, according to the farmers, led to reduced food and water intake and lying time and resulted in decreased milk production and increased risk of lameness. Many farmers perceived magnetic fields from, for instance, high-voltage lines, automated milking systems, or solar panels as possible causes for the behavior of their cows. Our aim for the study was to study potential factors such as magnetic fields and other factors such as barn climate and insect burden for adverse grouping behavior of dairy cows in the barn. For each case herd, 2 control herds were selected in the same postal area code. A case was a herd in which cattle grouped at least on 7 occasions in a month for several hours. In a control herd, the cows were in the barn during the same time period as in the matching case herd but did not show adverse grouping behavior. A questionnaire was administered by telephone in 31 case herds and 62 control herds. The questionnaire gathered information on behavior of the cows and potential risk factors. In addition, data on the distance of the herd to high-voltage lines was obtained. From a total of 74 variables, all variables with a P-value ≤0.10 were included in full multivariable logistic regression model. Backward selection was carried out at P ≤ 0.10. The grouping behavior of the cows started in most herds in June, was seen only during the day, and lasted mostly 6 to 8 h, with cows often grouped in the northern part of the barn. Identified risk factors appeared to be recently constructed barns, measured stray voltage in barns, and presence of fans in barns. Given the cross-sectional design of the case-control study, causality for these risk factors leading to adverse behavior of the cows could not be proven. Dissemination of the results to farmers hopefully results in measures that can prevent the unusual grouping behavior of cows.

Impact of paper bedding on lying behaviour and welfare in lactating dairy cows

Lying is a highly motivated behaviour in dairy cows. The level of comfort provided by the lying surface not only affects lying time, but can also affect several other aspects of welfare. We used a crossover design to compare shredded paper and sawdust bedding in relation to lying behaviour, activity, lameness, integument damage, cleanliness and productivity. Cubicles were bedded with a thin layer of bedding, which was replaced as necessary to retain its hygienic state. Twenty-eight lactating Holstein-Friesian dairy cows were divided into two balanced groups that experienced each bedding treatment for a 2 week period in opposing order. Paper bedding resulted in significantly less time spent lying down (paper: 45 %, SD ± 6.7, sawdust: 48 %, SD ± 7.3, P < 0.01). Paper had a more beneficial effect on lameness development than sawdust did (lameness decreased for eight cows and increased for one cow whilst on paper, whereas it decreased for one cow and increased for ten cows whilst on sawdust, P < 0.005). However, the magnitude of improvement in mobility score whilst on paper (0.5 points) was small compared to the deterioration seen whilst on sawdust (1–2 points). Bedding adhesion tended to be slightly increased during the paper bedding treatment (paper: 0.14 ± 0.16 SD, sawdust 0.03 ± 0.26 SD, P = 0.09). No treatment effect was observed for lying frequency, the speed of transitions towards lying, pre-transition intention movements, or the risk of collisions during the transition (P > 0.10). Furthermore, no significant effects on step count, damage to the integument, cleanliness or milk yields were observed (P > 0.10). Slips were too rare overall for analysis. These findings indicate that paper bedding and sawdust were mostly comparable in terms of impact on behaviour, welfare and productivity when provided on a short-term basis. Future trials are recommended to determine if treatment effects persist following prolonged exposure to paper bedding. Assessing the longitudinal development of lameness and the long-term impact of reduced lying time will be essential.

Virtual fencing technology to intensively graze lactating dairy cattle. II: Effects on cow welfare and behavior

Virtual fencing technology uses a neckband-mounted device to deliver an audio cue when an animal nears a virtual boundary that is set via a global positioning system, followed by an electrical stimulus if it crosses the boundary. The flexibility offered by this technology could revolutionize grazing management on dairy farms, but its application and effects on lactating dairy cattle have not been assessed. This experiment reports on the effects of an electric or a virtual front-fence on dairy cow behavior and welfare. Two temporally separated treatments were applied to a herd of 30 multiparous cows. Cows were provided an estimated 14 to 15 kg of dry matter/cow of fresh pasture in a new paddock every 24 h. From d 1 to 10 cows were grazed using a conventional electric front-fence (control treatment) and from d 14 to 23 they were grazed using a virtual front-fence (eShepherd, Agersens Pty Ltd.). Cows were trained to the technology from d 11 to 13. The milk production and live weight of individual cows were recorded daily. Cortisol concentrations were obtained from milk samples collected from individual cows on 3 d during each of the control and the virtual fence grazing periods, plus the first day of training. From d 6 of the experiment, 6 focal cows were fitted with a RumiWatch (Itin + Hoch GmbH) noseband sensor to monitor grazing and ruminating time, and 8 focal cows were fitted with an IceTag (IceRobotics Ltd.) sensors to monitor activity. Milk production, live weight, and the time cows spent standing and lying did not differ between the electric and virtual fence periods. Milk cortisol concentrations, activity, and the times spent ruminating and grazing were comparable between the electric and early virtual fence periods (i.e., d 1-3 with a virtual fence). However, at d 4 to 6 with a virtual fence, activity (steps taken and motion index) and time spent grazing were lower, and time spent ruminating was greater, compared with an electric fence. Further, least significant difference tests suggest milk cortisol concentrations were higher at d 5 with a virtual fence than at d 8 with an electric fence and d 1 with a virtual fence. We conclude there is no evidence of behavioral and welfare effects of virtual fencing on dairy cows in the days immediately following implementation of the technology in a simple intensive grazing regimen, but a longer study is required to fully elucidate effects beyond this period.

The effects of heat stress on the behaviour of dairy cows – a review

Abstract Heat stress in livestock is a function of macro- and microclimatic factors, their duration and intensity, the environments where they occur and the biological characteristics of the animal. Due to intense metabolic processes, high-producing dairy cows are highly vulnerable to the effects of heat stress. Disturbances in their thermoregulatory capability are reflected by behavioural, physiological and production changes. Expression of thermoregulatory behaviour such as reduction of activity and feed intake, searching for a cooler places or disturbances in reproductive behaviours may be very important indicators of animal welfare. Especially maintain of standing or lying position in dairy cattle may be a valuable marker of the negative environmental impact. Highly mechanized farms with large numbers of animals have the informatic system can detect alterations automatically, while small family farms cannot afford these type of equipments. Therefore, observing and analysing behavioural changes to achieve a greater understanding of heat stress issue may be a key factor for developing the effective strategies to minimize the effects of heat stress in cattle. The aim of this review is to present the state of knowledge, over the last years, regarding behavioural changes in dairy cows (Bos Taurus) exposed to heat stress conditions and discuss some herd management strategies provided mitigation of the overheat consequences.