This article concerns the effect of strain accumulation during low-cycle fatigue on the post-fatigue tensile behavior of metal matrix composites (MMCs) with identical layers of aluminum reinforced by Fe3O4 nanoparticles produced via the accumulative roll bonding (ARB) technique. Work-hardening behavior, deduced from ratcheting curves, was evaluated by the post-fatigue tensile test. The key finding in the mechanical properties of homogeneous nanocomposite in terms of reinforcement distribution, evolving due to microstructural evolution during the ARB process, is that accumulation of ratcheting strain decreases, and fatigue and post-fatigue tensile behavior significantly increases. The rupture mechanism, analyzed by scanning electron microscope, revealed that deep hemispherical-shaped dimples, a well-known feature of ductile rupture, were changed to elongated shallow shear dimples by the impact of microstructural evolution due to imposed 1000 cycles of ratcheting test.