Spectral fingerprinting has emerged as a powerful tool for unraveling intricate interactions between the intracellular environment and nanomaterials. This study leverages spectral fingerprinting to deepen our comprehension of the interplay between macrophages and nanotubes, utilizing their Near Infrared fluorescence to discern cellular characteristics. This study employs Raman microscopy to showcase significant differences in defect ratio and intracellular processing of nanotubes, among macrophage phenotypes. The near infrared features of DNA-SWCNTs serve as distinctive markers for identifying different macrophage phenotypes. Here we use a support vector machine learning model to accurately identify M1 and M2 macrophages, achieving an impressive accuracy of 95%. The implications of this research extend to the development of nanomaterial-based platforms for cellular identification, holding promise for potential applications in real time in-vivo cell differentiation monitoring.This study underlines the pivotal role of nanotubes in delineating the diverse micro-environments of distinct macrophage phenotypes for clinical research. It emphasizes the potential of short DNA sequences and machine learning in characterizing and classifying cell phenotypes, offering valuable insights into the intersection of nanotechnology and biomedical research.This innovative approach holds great promise in advancing our understanding of dynamic cellular processes, disease states, disease progression, and response to stimuli in-vivo. The platform’s sensitivity to minute changes in the Near Infrared spectrum positions it as a valuable tool for researchers and clinicians. It facilitates the real time monitoring of cellular behavior within living systems, offering insights that extend to both fundamental research and clinical applications. The implications of this research pave the way for future developments in nanotechnology and biomedicine, bridging the gap between spectral analysis and cellular dynamics for enhanced diagnostic and therapeutic interventions. Figure 1
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