Beetle Species Research Articles

Feeding patterns of two sympatric species of Tortoise beetle (Coleoptera: Chrysomelidae)

During a pilot survey, the feeding patterns of Aspidomorpha miliaris (Fabricius), and A. sanctaecrucis (Fabricius) beetles were observed, compared and interpreted. Questions were raised for further study.

Correlation between Coleoptera abundance in cattle excrements and pesticide contamination of nature conservation areas grazed by cattle

The aim of this study was to explore the relationship between pesticide contamination at 16 locations in 14 Dutch nature conservation areas and the abundance of Coleoptera (among which dung beetles) in excrements of grazing cattle. A wide spectrum of pesticides was measured in soil, vegetation and excrements of cattle, obtained from all locations. In the fresh dung pats sampled for chemical analysis, beetle numbers were counted and beetle species were identified. In total, 31 different pesticides (including some metabolites) were detected: 14 in fresh excrements, 17 in soil and 20 in vegetation. Total pesticide concentrations in soil, vegetation and excrements varied between 2.6 and 200 μg kg −1 dry matter. In vegetation, the most frequently encountered classes of pesticides (including some of their metabolites) were fungicides (9), herbicides (4) and insecticides (6). The total number of Coleoptera beetles in dung pats correlated negatively with the total concentration of insecticides in vegetation (Kendall's τ −0.501 at p < 0.05). The total concentrations of herbicides and fungicides were not statistically significant correlated with Coleoptera beetle numbers in the dung pats. Yet, the concentration of one single herbicide, viz. chlorpropham in vegetation correlated significantly negative with Coleoptera counts (Kendall's τ −0.603 at p < 0.01).

Monitoring arthropods under the scope of the LIFE-BEETLES project: I - Baseline data with implementation of the Index of Biotic Integrity.

The urgent need for conservation efforts in response to the global biodiversity crisis is exemplified by initiatives, such as the EU LIFE BEETLES project. This project aims to preserve endangered arthropod species that are crucial for ecosystem functionality, with a focus on endemic beetle species in Flores, Pico and Terceira Islands (Azores, Portugal): Tarphiusfloresensis Borges & Serrano, 2017, Pseudanchomenusaptinoides (Tarnier, 1860) and Trechusterrabravensis Borges, Serrano & Amorim, 2004. These species are single island endemics respectively from Flores, Pico and Terceira. They are threatened by environmental degradation, facing the dual challenge of restricted distribution and habitat degradation due to the spread of invasive plants.The project aims to enhance habitat quality and biodiversity conservation through habitat restoration and plant invasive species control measures. These measures are funded by the European Commission and coordinated by the Azorean Environment Directorate-General. The current Data Paper evaluates the effectiveness of the LIFE BEETLES project in improving habitat quality and offers insights into the balance between habitat restoration efforts and endangered species conservation in island ecosystems, utilising as ecological indicator the Index of Biotic Integrity (IBI) framework. This study establishes a comprehensive database derived from a long-term arthropod monitoring survey that used SLAM (Sea, Land and Air Malaise) traps and pitfall traps. Our findings present a proxy for assessing the overall habitat quality for endemic invertebrates, using arthropods as main indicators.From September 2020 to June 2023, a total of 31 SLAM traps were monitored. The traps were set up as follows: seven in Flores (three in mixed forest and four in native forest), 10 in Pico (four in mixed forest and six in native forest) and 14 in Terceira (three in mixed forest and 11 in native forest). Traps were monitored every three months.In addition, we surveyed the epigean fauna in 19 transects with 15 non-attractive pitfall traps per transect. The transects were set up during two weeks at the end of August every year between 2020 and 2023. Eight transects were established in Flores, consisting of one in pasture, four in mixed forest and three in native forest. Six transects were established in Pico, consisting of two in pastures and four in native forest. Five transects were established in Terceira, consisting of two in mixed forest and three in native forest.A total of 243 arthropod taxa were recorded, with 207 identified at the species or subspecies level. These taxa belonged to four classes, 24 orders and 101 families. Out of the 207 identified taxa, 46 were endemic, 60 were native non-endemic, 80 were introduced and 21 were of indeterminate status. Habitat information is also provided, including general habitat and dominant species composition. This publication contributes to the conservation of highly threatened endemic beetles by assessing habitat quality, based on arthropod communities and habitat description (e.g. native or exotic vegetation).Using the Index of Biotic Integrity (IBI) to comparing pre- and post-intervention data, we found no significant change within the epigean community. In contrast, the understorey community sampled with SLAM traps experienced a slight global decrease in biotic integrity over the study period. These findings suggest that the short duration of the study may not be sufficient to detect significant changes, as ecosystem recovery often requires long-term monitoring. The observed changes in the understorey community may be attributed to disturbances from intervention activities, highlighting the need for ongoing monitoring to assess long-term ecological resilience and recovery.

Xerothermophilous species of rove beetles (Coleoptera: Staphylinidae) in dry-warm habitats prefer their wetter niches

Xerothermophilous species of rove beetles (Coleoptera: Staphylinidae) in dry-warm habitats prefer their wetter niches

Species composition of dung beetles (Scarabaeiade: Scarabaeinae) from different habitat types in a protected area in eastern Africa

AbstractUnderstanding the composition and distribution of dung beetle species across various habitat types is crucial for elucidating their ecosystem functions. Dung beetles play pivotal roles in ecological processes such as nutrient cycling, seed dispersal and parasite suppression. Despite numerous studies on the influence of habitat types on dung beetle communities, knowledge gaps persist, particularly on how these beetles respond to different vegetation types in protected areas. In this study, we surveyed dung beetle species in Nyerere National Park, Tanzania, using baited pitfall traps across four habitat types: closed miombo woodland, open miombo woodland, marshland and riverine. We identified a total of 5534 individuals representing 63 species, 29 genera and nine tribes. Significant variations in species diversity were observed among the habitats, with the closed miombo woodland exhibiting the highest diversity and the open miombo woodland the lowest. Notably, certain species, such as Euoniticellus intermedius, Euonthophagus carbonarius, Gymnopleurus ignites, Onitis alexis, Onthophagus, Onthophagus vinctus, Onthophagus plebejus and Sisyphus goryi displayed a generalist distribution across all habitat types. Our findings underscore the critical importance of maintaining and conserving the quality of diverse habitat types to ensure the long‐term preservation of dung beetle biodiversity and the ecosystem services they provide. This study contributes valuable insights into dung beetle ecology and offers practical implications for ecosystem management and conservation strategies.

A brief overview of the entomofauna of lamellar beetles (Scarabaeidae) of the foothills of the Maly Chimgan (Republic of Uzbekistan)

During July–August 2016, the entomofauna of lamellar-whiskered coleoptera was studied in the Republic of Uzbekistan, in the Bostanly District, in the foothills of the Maly Chimgan, in the vicinity of the village. Chimgan. During a short-term expedition in 2016, we identified 18 species of lamellate beetles belonging to 6 subfamilies (Aphodiinae, Cetoniinae, Dynastinae, Melolonthinae, Rutelinae, Scarabaeinae) and 10 tribes (Adoretini, Aphodiini, Cetoniini, Coprini, Gymnopleurini, Melolonthini, Oniticellini, Onitini, Onthophagini, Oryctini). Taxonomically, among these groups, the Onthophagini tribe contained the most species – 5 species (27,77%), Cetoniini tribe – 4 species (22,22%), Aphodiini tribe – 2 species (11,11%). One species (5,55%) included the tribes Adoretini, Coprini, Gymnopleurini, Melolonthini, Oniticellini, Onitini, Oryctini. By trophic specialization, coprophages prevailed (61,11%), followed by phytophages (33,33%), and aphages were less represented (5,55%). The identification of Melolontha hippocastani hippocastani (Fabricius, 1801) here can probably cause discussion, since according to S.I. Medvedev’s southern border of this species passed through northern Kazakhstan. Although it can be assumed that over a period of almost 70 years, the boundaries of the habitats of many species have changed significantly.

New records of Cassidinae and Cerambycidae (Coleoptera: Chrysomeloidea) from Baturité Massif, Brazil, with the description of a new species of Charidotis (Coleoptera: Chrysomelidae: Cassidinae)

The Baturité massif, situated in Ceará state’s central hinterland, is one of the largest humid mountain ranges in the Brazilian semi-arid region. Flora and vertebrate surveys have been conducted in the region, revealing endemic species, as well as a combination of fauna and flora from neighboring biomes, the Atlantic and Amazon rainforests. However, invertebrate inventories have been scarce, leaving a significant portion of the region’s biodiversity unexplored. This paper presents the outcomes of collaborative expeditions conducted by the recently established Museu de História Natural do Ceará Prof. Dias da Rocha and Museu Nacional/Universidade do Rio de Janeiro to fill this knowledge gap by promoting surveys to document the region’s biodiversity. As a result, one new species of tortoise beetle is described from the massif, Charidotis rochai Simões and Sekerka, new species. Additionally, we report thirteen new state records for the beetle subfamilies Cassidinae (Chysomelidae), and Cerambycinae and Lamiinae (Cerambycidae). Among these records, eight genera are documented for the first time in the state of Ceará, and the species Centrocerum variatum (Newman, 1841) constitutes the first record in the northeastern region of Brazil. These findings underscore the significance of survey initiatives and the need for comprehensive biodiversity study within the Baturité massif.

Development of the Pronotal Explanate Margin, a Novel Evolutionary Trait in Tortoise Beetles.

Most tortoise beetles, belonging to the subfamily Cassidinae (Coleoptera: Chrysomelidae), possess distinctive explanate margins, comprising elongations of the pronotum and elytra outer margins. These margins flatten against the ground, serving as a unique defensive mechanism against predators. To understand the developmental and evolutionary origins of explanate margins, we examined the development of the pronotal part of these structures in two tortoise beetle species: Thlaspida biramosa (Boheman) (tribe Cassidini) and Laccoptera nepalensis (Boheman) (tribe Aspidimorphini). Although final (fifth) instar larvae of both species exhibited no external prothoracic structures associated with explanate margins, pupae possessed a plate-shaped structure projecting anterolaterally on their pronotum. This plate-shaped structure was identified as the pupal primordium of the pronotal explanate margin, as the explanate margin emerged from inside the structure during eclosion. In prepupae of T. biramosa, the primordial tissue exhibited three region-specific folding and furrowing patterns, beneath larval cuticles. These epithelial structures expanded within minutes at the onset of pupation, resulting in pupal primordial formation. Thus, pronotal explanate margins originate from pronotal epithelia, with the furrowing and folding patterns of the epithelia shaping pupal and even adult pronotal explanate margins. The presence of morphologically similar pupal pronotal projections in various Cassidinae suggests that the development of pronotal explanate margins is likely common in these beetles.

Alpha and beta‐diversity and community structure of dung beetle assemblages in an East African protected savannah

AbstractFew studies have examined how dung beetle species composition changes across several mammalian dung types. Species composition and abundance of dung beetles were studied in dung samples of seven African mammals along animal trails and using 14 baited‐pitfall traps in both the park and the buffer zone resulting in a combined data set of 113 samples. Both the number of species (18) and the number of individual dung beetles (600) encountered were low for an area close to the Equator. Few species (2) and herbivores (13) tunnellers (9) dominated the dung beetles community, feeding and guild structure respectively. Alpha diversity was moderate 2.06 (1 &lt; H′ ≤ 3). Beta diversity ranged from low 0.8 (high similarity) to high (zero, no similarity). Dung beetle species composition varied with dung type with the degree of pairwise similarity between two dung types in 19 of the total (21) dung beetle communities comparisons showing low similarity (0.4) to no similarity (zero). The observed discrepancies may be attributed to dung size, composition and consistency as well as to the low number of dung of the different animal species sampled. Furthermore, further research is needed as rarefaction curves suggest that sampling was incomplete.

Interspecific difference and frequency dependence in habitat use of coexisting Ohomopterus ground beetle species under reproductive interference

Interspecific difference and frequency dependence in habitat use of coexisting <i>Ohomopterus</i> ground beetle species under reproductive interference

An investigation of the insect component in the diet of the Grey Heron Ardea cinerea and Little Egret Egretta garzetta

ABSTRACT Capsule Aquatic insects made up a relatively small part of the Grey Heron’s diet, while Little Egrets consumed numerous beetles, both aquatic and terrestrial. Aims To explore the extent of insect predation by Grey Herons and Little Egrets. Regurgitated pellets were collected from beneath trees in which herons and egrets were nesting. Methods Seventy Grey Heron and 48 Little Egret pellets were collected from the RSPB reserve at Northward Hill, Kent. These pellets were disaggregated and prey items were collected and sorted under a binocular microscope. Results Grey Herons fed on a relatively small number of aquatic beetles (Coleoptera: seven species, 32 individuals) and bugs (Hemiptera: two species, six individuals). Little Egrets, in contrast, fed on a diverse array of beetle species, both aquatic (three families, 11 species, 48 individuals) and terrestrial (10 families, 35 species, 111 individuals), and also a few aquatic bugs (one family, one species, three individuals). The extent of terrestrial beetles in the diet constitutes new information for Little Egrets. Conclusion Insects, both terrestrial and aquatic, represent an important component of the diet of Little Egrets. For Grey Herons, insects are a very minor component of the diet, involving aquatic species only.

Antifungal capabilities of gut microbial communities of three dung beetle species (Scarabaeidae: Scarabaeinae).

Gut microbial communities are part of the regulatory array of various processes within their hosts, ranging from nutrition to pathogen control. Recent evidence shows that dung beetle's gut microbial communities release substances with antifungal activity. Because of the enormous diversity of gut microorganisms in dung beetles, there is a possibility of discovering novel compounds with antifungal properties. We tested the antifungal activity mediated by gut microbial communities of female dung beetles against nine phytopathogenic fungi strains (Colletotrichum asianum-339, C. asianum-340, C. asianum-1, C. kahawae-390, C. karstii-358, C. siamense-220, Fusarium oxysporum-ATCC338, Nectria pseudotrichia-232, Verticillium zaelandica-22). Our tests included the gut microbial communities of three species of dung beetles: Canthon cyanellus (roller beetle), Digitonthophagus gazella (burrower beetle), and Onthophagus batesi (burrower beetle), and we followed the dual confrontation protocol, i.e., we challenged each fungal strain with the microbial communities of each species of beetles in Petri dishes containing culture medium. Our results showed that gut microbial communities of the three dung beetle species exhibit antifungal activity against at least seven of the nine phytopathogenic fungal strains. The gut microbial communities of Onthophagus batesi significantly decreased the mycelial growth of the nine phytopathogenic fungi strains; the gut microbial communities of Canthon cyanellus and Digitonthophagus gazella significantly reduced the mycelial growth of seven strains. These results provide a basis for investigating novel antifungal substances within gut microbial communities of dung beetles.

Epigeic spider and ground beetles (Carabid) communities of semi-natural and natural habitats in agricultural landscape in Slovakia

Abstract We studied the spatial structure of spiders and beetles in 4 types of habitats in the Poloniny National Park. Between the years 2020 and 2021, we recorded 1,597 beetle specimens and 3,765 spider specimens using the pitfall trap method. The most represented beetles were Poecilus versicolor (53.4%), Nebria brevicollis (10.3%), Staphylinus caesareus (4.4%) and Amara familiaris (4.1%). Among the spiders, there were species Pardosa pullata (21.81%), Pachygnatha degeeri (14.21%), Xerolycosa nemoralis (10.28%), Pardosa palustris (7.86%), Trochosa ruricola (6.24%) and Alopecosa pulverulenta (4.38%). We confirmed the occurrence of two European important species of beetles (Carabus zawadzkii, Carabus variolosus) and 3 species of spiders belonging to the vulnerable species according to the IUCN threat status (Gongylidiellum vivum, Palliduphantes milleri and Xysticus lineatus). We also found the spider Micaria micans, which is new to the Slovak fauna and information about its distribution and habitat preferences in Slovakia is presented. Using multivariate analyzes (CCA, RDA) and classification species into bioindicative groups, we found a greater connection of adaptable and eurytopic species of ground beetles to the mesophilic meadow and of spiders to the habitat Nardetum pasture. Based on bioindicative groups of species (adaptable, eurytopic and relict), we found only a small difference in percentile points between spiders and beetles.

Debarking harvesters simultaneously combat the European spruce bark beetle (Ips typographus) and conserve non‐target beetle diversity

Abstract In the face of climate change, the European Spruce Bark Beetle (Ips typographus) breeding predominantly in Norway spruce (Picea abies) led to exceptional amounts of damaged timber in European forests. Up to now, if pest control is applied, damaged or weakened P. abies trees are either extracted by salvage logging or, when quantities are low, made unsuitable for breeding by manual debarking techniques. Both pest control interventions are costly, are often limited by the short timeframe of effectiveness and come with negative impacts on the non‐target biodiversity. As alternatives for timely removal, a debarking head for harvesters for large scale disturbances and a bark gouging device for motor‐manual treatment have been developed in recent years to make breeding material unsuitable for bark beetles and reduce existing larvae. Based on data from an experimental design with infested Norway spruce logs, we show that the harvester debarking head and the motor‐manual bark gouging regulate I. typographus populations efficiently, whereas a conventional harvester did not reduce the emerging bark beetles. Species assemblages of non‐target beetles living in the infested Norway spruce logs were altered from the natural species assemblages in control logs by processing logs with the debarking head or the bark gouging device but not by the conventional harvester. None of the bark treatments reduced non‐target beetle species richness in this experiment. Practical implication. We endorse the debarking head and bark gouging as alternatives to salvage logging and manual debarking. This uncouples pest control from in‐time dependencies on the availability of transport capacities. The debarking head and bark gouging open up the opportunity to retain dead wood biomass in the forest, supporting ecological benefits and conservation goals. Particularly for protected areas these two new management options better balance requirements of pest control and biodiversity conservation.

Structure and composition of a canopy-beetle community (Coleoptera) in a Neotropical lowland rainforest in southern Venezuela.

Species richness, community structure and taxonomic composition are important characteristics of biodiversity. Beetle communities show distinct diversity patterns according to habitat attributes. Tropical rainforest canopies, which are well known for their richness in Coleoptera, represent such a conspicuous life zone. Here, I describe a canopy-inhabiting beetle community associated with 23 tree species in a Neotropical lowland rainforest. Adult beetles were sampled manually and in aerial traps using a large tower crane for a cumulative year. The sample revealed 6738 adult beetles, which were assigned to 862 (morpho-)species in 45 families. The most species-rich beetle families were Curculionidae (n = 246), Chrysomelidae (n = 121) and Cerambycidae (n = 89). The most abundant families were Curculionidae (n = 2746) and Chrysomelidae (n = 1409). Dominant beetle families were found in most assemblages. The beetle community consisted of 400 singletons (46.4%). A similar proportion was evident for assemblages of single tree species. I found that 74.5% of all beetle species were restricted in their occurrence on host trees to the phenological season and time of the day. This daily and seasonal migration causes patterns similar to mass effects and therefore accounts for the high proportion of singletons.

First report of dung beetle species of genus Onthophagus (Coleoptera: Scarabaeidae) from Valmiki Tiger Reserve, Bihar, India

First report of dung beetle species of genus Onthophagus (Coleoptera: Scarabaeidae) from Valmiki Tiger Reserve, Bihar, India

Fires and Clear-Cuttings as Local Areas of Arthropod Diversity in Polar Regions: Khibiny Mountains

The well-known phenomenon of attracting untypical animals to disturbed territories has been poorly investigated in the polar mountains. We studied arthropod diversity in self-healing industrial clear-cuts and burn areas in the Khibiny Mountains, Kola Polar region. Fieldworks were conducted at four sites, including a control mountain taiga forest and its three transformed variants: burnt forest, uncleared clear-cut, and twice-disturbed burnt clear-cut. Arthropods were collected using formalin traps 2–3, 5–6, and 8–9 years after industrial deforestation in 2012 and an extensive grass-roots fire in 2013. Out of 124 identified species (spiders—61; ground beetles—41; and rove beetles—22), 79 (or 64%) were collected in disturbed, primarily burned areas and were absent in control forest. We note ten species of rove beetles, nine species of ground beetles, and eight species of spiders for the first time in the well-studied arthropod fauna of the Khibiny Mts. We found that grass-root fires transform the soil vegetation covers in the polar mountain forests more powerfully in comparison with extensive deforestation and attract a greater diversity of arthropods with different preferences, enriching the fauna of the polar mountains and the Subarctic region as a whole. The attraction effect persists for, at least, a decade after the violations.

New species and records of subgenus Aethina from China (Coleoptera: Nitidulidae)

Two new species of sap beetles, A. (A.) similinconspicua sp. nov. and A. (A.) shizhuica sp. nov., of the subgenus Aethina Erichson, 1843 are described and illustrated from southwestern China. And four new species, A. (A.) cyaneipennis Grouvelle, 1903, A. (A.) dubitabilis Kireitshuk, 1986, A. (A.) kirejtshuki Jelínek, 1995, and A. (A.) lindskogi Jelínek &amp; Kirejtshuk, 1986 are recorded for the first time from China.

Bark beetles on logging residues of European larch: Effects of shading and diameter of logging residues on infestation density

Abstract Ips cembrae, an important pest of European larch (Larix decidua), has caused local outbreaks in the last two decades and is becoming increasingly important as the proportion of European larch in forests increases. In 2021–2023, larch logs and piles of branches were placed into shaded forest and sunlit areas every month to study bark beetle species on logging residues. After adult emergence, the logs and branches were debarked, and the infestation density of bark beetles was analysed. The results indicate that shading, log diameter and top/bottom parts of log had a significant effect on infestation density of I. cembrae on logs. The infestation density of I. cembrae was higher on logs felled between December and June than on logs felled between July and November. Shading of branches was the most significant variable affecting the bark beetle species composition on branches. All four of the bark beetle species recorded were affected by diameter of the branches. Ips cembrae primarily infested sunlit branches while Cryphalus intermedius preferred shaded branches. In contrast, the infestation densities of Pityogenes chalcographus and Pityophthorus pityographus seemed to be affected primarily by moisture content of the branches.

Environment-Mediated Vertical Transmission Fostered Uncoupled Phylogenetic Relationships between Longicorn Beetles and Their Symbionts.

The Coleoptera Cerambycidae (longicorn beetles) use wood under different states (living healthy, freshly snapped, completely rot, etc.) in a species-specific manner for their larval diet. Larvae of some Cerambycidae groups have mycetomes, accessory organs associated with the midgut that harbor fungal symbiont cells. The symbionts are thought to improve nutrient conditions; however, this has yet to be shown experimentally. To deduce the evolutionary history of this symbiosis, we investigated the characteristics of the mycetomes in the larvae of longicorn beetles collected in Japan. Lepturinae, Necydalinae, and Spondylidinae are the only groups that possess mycetomes, and these three groups' mycetomes and corresponding fungal cells exhibit different characteristics between the groups. However, the phylogenetic relationship of symbiont yeasts does not coincide with that of the corresponding longicorn beetle species, suggesting they have not co-speciated. The imperfect vertical transmission of symbiont yeasts from female to offspring is a mechanism that could accommodate the host-symbiont phylogenetic incongruence. Some Lepturinae species secondarily lost mycetomes. The loss is associated with their diet choice, suggesting that different conditions between feeding habits could have allowed species to discard this organ. We found that symbiont fungi encapsulated in the mycetomes are dispensable for larval growth if sufficient nutrients are given, suggesting that the role of symbiotic fungi could be compensated by the food larvae take. Aegosoma sinicum is a longicorn beetle classified to the subfamily Prioninae, which does not possess mycetomes. However, this species contains a restricted selection of yeast species in the larval gut, suggesting that the symbiosis between longicorn beetles and yeasts emerged before acquiring the mycetomes.