Beef Breeds Research Articles

Slaughter qualities and by-product yield in Limousin bull calves of different genotypes

The study aimed to provide a comparative analysis of post- slaughter performance and by-product yield obtained from purebred Limousin bull calves of different genotypes. The purposes of the study were to fatten bull calves, offspring of mothers of different origin, as well as carcasses and by-products obtained as a result of their control slaughter. The bull calves were raised according to the technology of beef cattle breeding with elements of resource saving. The control slaughter of the bull calves was carried out when they reached the required meat condition at the age of 18 months. In the course of the research, it was established that the bull calves obtained from mothers of French selection significantly exceeded (p˂0.01) the peers obtained from mothers of Australian origin in live pre- slaughter weight of 34.6 kg (6.5%), in carcass weight of 30.8 kg (9.8%); in slaughter weight of 30,6 kg (9.4%). Bulls derived from local selection mothers had significantly (p˂0.05) higher slaughter weight by 16.3 kg (4.4%) compared to peers whose mothers were of Australian origin. Significant differences between groups in weight and yield of byproducts were not revealed; however, there was a tendency to increase the weight of organs in the groups of animals with high preslaughter live weight (bull calves of the first and third experimental groups). Based on the obtained data, it can be assumed that breeding of the ancestors of the studied animals for a long period of time under conditions of different breeding, housing and feeding technologies influences the meat qualities of carcasses obtained by their offspring, Limousin bull calves, which are descendants of mothers of different origin.

Effectiveness of the use of the Vytelle system in evaluating bulls in Kazakhstan.

This article presents the results of the introduction of Canadian digital technologies Vytelle GrowSafe in the beef cattle breeding of Kazakhstan to assess Residual Feed Intake (RFI). The experiments were carried out with Qazaq Aqbas Breed in the Privet Farms of North Kazakhstan (Akmola and Pavlodar farms). As part of the ration in Akmola farm for a bull of 350 kg of weight, are contained 77 MJ or 7.6 kg of DM, in Pavlodar farm for a bull weighing 290 kg contains 65 MJ or 6.5 kg of DM. Studies have shown that in the Akmola farm the RFI index ranged from -2.08 to +2.88, in the Pavlodar farm from -1.89 to +2.06. According to the results of research in the Akmola farm, the RFI assessment show, 7 out of 26 bulls are suitable for breeding sale, in the Pavlodar farm out of 55 bulls 18. The assessment of the productive effect of feed on RFI, mainly (by 80%), coincided with the assessment of the ratio of gain to consumed dry matter, and some bulls showed deviations in terms of residual average daily live weight gain and the corrected value of the ratio of feed costs to live weight gain, which led to incomplete manifestation of the productive potential of the consumed feed.

ASSESSING OF KAPPA-CASEIN POLYMORPHISM IN UKRAINIAN CHAROLAIS CATTLE AND ITS ASSOCIATIONS WITH PRODUCTIVITY TRAITS

Much attention is paid to the study of kappa-casein gene polymorphism in dairy breeds of cattle. Moreover, there is a lack of research on kappa-casein polymorphism in cattle beef breeds. Knowing that different alleles of the kappa-casein gene have different effects on milk yield and milk protein content, it would be important to study the exact allele associations in Ukrainian Charolais cattle. In addition, it would be relevant to find out whether there is an effect of different alleles of the kappa-casein gene on growth parameters in offspring. In addition, the current study would be highly relevant due to no previous research of κ- Cn in Ukrainian Charolais cattle. The polymorphism of the kappa-casein (κ-Cn) gene was studied in the population of Ukrainian Charolais cattle (n=29), "Privilla" agricultural company (Ukraine, Luhansk region) using the PCR-PDRF method. DNA was extracted from blood using the DNA Sorb isolation kit (AmplySens). Hind III restriction enzyme (FastDigest, Thermo Scientific) was used to see 2 allelic variants of κ-Cn polymorphism, which are A (273 bp) and B (182, 91 bp). The frequency of the A allele was 0.57±0.065 and 0.43±0.065 of the B allele. According to the genotyping results, allele frequency distribution in the population of 2021 did not reliably differ from the population of 2012. As a result, allele frequencies of the kappa-casein gene in 2012 for allele A was 0.61±0.054 and for B 0.39±0.054. This indicates the lack of selection pressure on population dynamics such as selective selection and gene drift over a period of 10 years. The frequency of AA genotypes was equal to 0.31, of BB genotype to 0.17 and of AB to 0.52. It was found that the theoretically expected number of genotypes, calculated according to the Hardy-Weinberg principle, did not reliably differ from the actual number. It could be related to current alleles being within an equilibrium state. In cattle with different genotypes of the κ-Cn gene, the values of the liveweight gain (kg) and the average daily gain (g) were calculated. In cattle with the BB genotype, there was an increase in the weight gain of their calves at weaning at 210 days (206.0±5.65 kg). In addition, the average daily gain of calves was 981.0±26.94 g, compared to genotypes AA (201.4±8.08 kg and 958.9±37.85 g, respectively) and AB – (196.8±2.45 kg and 936.9±11.73 g, respectively). Though, there were no significant differences between AA, BB and AB genotypes considering the studied parameters. Keywords: gene, kappa-casein, cattle, Charolais, polymorphism, meat productivity.

Selection for yearling growth rate in Angus cattle results in bigger cows that eat more

Context Measurement of weight provides the basis of most performance-recording schemes for beef cattle around the world. The limitation of faster growth rate as a breeding objective, without considering changes in mature-cow weight, is the expected increase in cow size and, hence, feed requirements. Aims To measure the correlated changes in feed intake and efficiency of cows, calves and the cow–calf unit following divergent selection for growth rate. Methods The cows and their calves came from three lines of Angus cattle selected for either fast weight gain to yearling age (the High-line), slow weight gain (the Low-line), or from an unselected Control-line. Efficiency was evaluated over an annual production cycle. Individual cow weights and feed intakes, and calf growth and feed intake (including milk), were recorded. Milk production, milk composition and body composition were also measured so that correlated changes in efficiency of use of energy and nitrogen could be determined. Key results The High-line cows were 18% (P < 0.05) heavier than the Low-line cows at the start and consumed 7% (P < 0.05) more feed than did the Low-line cows. Feed efficiency of the cow–calf unit was 12% higher (P < 0.05) in the High-line cows and calves than in the Low-line cows and calves. When compared on the basis of feed used relative to their weight and weight gain there was no difference (P > 0.05) between the selection lines. Divergent selection was accompanied by a change in body composition, with the High-line cows containing proportionally less protein and more fat in their bodies than did the Low-line cows. There was no evidence for change in the efficiency of feed energy use, but there was a 10% (P < 0.05) improvement in nitrogen efficiency of the cow–calf unit in the High-line compared with the Low-line. Conclusions Divergent selection for weight gain led to a correlated change in cow size and cow feed requirements. Implications This experiment supported the consensus among earlier reviews that there is little evidence that selection for growth rate or size, without moderating change in mature-cow weight, is associated with improved efficiency of feed energy use in maternal beef breeds.

Dampak Covid-19 terhadap Tingkat Kecemasan dan Kinerja Keluarga Peternak Sapi Potong pada Peternakan Rakyat di Provinsi Nusa Tenggara Barat

Data on the development of the Covid-19 pandemic as of May 17 2020 shows that all areas in West Nusa Tenggara Province are declared as red zones. This condition causes people to become anxious. One community group that feels this anxiety is beef cattle breeders on smallholder farms. The purpose of this study was to determine the impact of the Covid-19 pandemic on the anxiety level of beef cattle farming families on smallholder farms in West Nusa Tenggara Province. The research method is a survey method from May to September 2022 in 10 regencies/cities throughout West Nusa Tenggara Province. There are 20 parameters that are measured as indicators of anxiety levels. Anxiety levels are measured with a Likert scale of 1-5. Data was tabulated using Microsoft Excel and then analyzed descriptively. The results showed that the impact of the Covid-19 pandemic was most felt by the farmers' wives with an average anxiety level of 97.4%; farmer's children 94.0%; and father as the head of the family 93.3%. The performance of beef cattle breeders is measured by seven indicators showing that 81.5% of beef cattle raising families do not stay at home all day long, but 70.4% leave the house to work in the fields, gardens and fields, and earn a living for their families. The conclusion is that all members of the beef cattle farmer's family feel anxious about the Covid-19 pandemic. The farmer's wife is the member of the family who feels the most anxiety. Nonetheless, the Covid-19 pandemic did not have a negative impact on the performance of beef cattle farming families on smallholder farms in West Nusa Tenggara Province.

Genetic Correlations between Days to Calving across Joinings and Lactation Status in a Tropically Adapted Composite Beef Herd

Female fertility is essential to any beef breeding program. However, little genetic gain has been made due to long generation intervals and low levels of phenotyping. Days to calving (DC) is a fertility trait that may provide genetic gain and lead to an increased weaning rate. Genetic parameters and correlations were estimated and compared for DC across multiple joinings (first, second and third+) and lactation status (lactating and non-lactating) for a tropical composite cattle population where cattle were first mated as yearlings. The genetic correlation between first joining DC and mature joining DC (third+) was moderate–high (0.55–0.83). DC was uncorrelated between multiparous lactating and non-lactating cows (rG = −0.10). Mature joining DC was more strongly correlated with second joining lactating DC (0.41–0.69) than with second joining non-lactating DC (−0.14 to −0.16). Thus, first joining DC, second joining DC and mature joining DC should be treated as different traits to maximise genetic gain. Further, for multi-parous cows, lactating and non-lactating DC should be treated as different traits. Three traits were developed to report back to the breeding programs to maximise genetic gain: the first joining days to calving, the second joining days to calving lactating and mature days to calving lactating.

Classification of cattle breeds based on the random forest approach

The determination of breed composition or the population of origin has several practical applications, including the authentication of brand mono-breed products that have recently been developed in several livestock species, including cattle. Therefore, the aim of the present study was to verify the applicability of a random forest (RF) approach to distinguish between different dairy and beef cattle breeds based on a reduced panel of single nucleotide polymorphisms (SNPs). A total of 1,370 animals from 17 cattle breeds were genotyped with a 50 K SNP microarray. After SNP pruning, 5,296 SNPs were retained for further analysis. Six methods were used for determining panels of the most informative SNPs: Wright's fixation index (FST), principal component analysis (PCA), random forest (RF) based on the Gini index (GI; two variants) and RF based on the mean decrease in accuracy (MDA; two variants). There was a similar distribution of selected SNPs per BTA, especially for the FST and PCA methods. Two panels of 96 SNPs obtained with two variants of RF-GI and RF-MDA contained the same SNPs but with a different ranking. The percentage of correct classification on the test set (10% of cases randomly selected from the whole dataset) was 100% for the Angus, Hereford, Holstein, Brown Swiss, and Jersey breeds. For the Simmental, Piedmontese, Romagnola, Shorthorn, Norwegian Red, Charolais, Cika, Tyrol Grey, Limousin, Austrian Pinzgau, Slovak Pinzgau, and Slovak Spotted breeds, it was 75.00 – 87.50%, 0.00 – 66.67%, 66.67 – 100.00%, 80.00 – 100.00%, 0.00 – 50.00%, 75.00 – 100.00%, 0.00 – 66.67%, 90.91 - 100.00%, 91.67%, 81.82 - 100.00%, 93.33 - 100.00%, 55.56 - 100.00%, respectively. The overall correct classification rate (for all breeds together) was 87.14%, 94.29%, 90.00% and 88.57% for RF-GI, RF-MDA, FST and PCA, respectively. Consequently, the most accurate method for distinguishing between cattle breeds was RF based on the MDA, although the differences amongst models were not large.

Research on Technical Efficiency of Feed Use for Sustainable Beef Cattle Breeding in China: Evidence from 169 Beef Cattle Farmers

Sustainable green development, cost saving, and efficiency improvement have become the main theme of the high-quality development of China’s animal husbandry and the problem of overuse of feed should be paid more attention. Based on the 3-year input–output data of 169 beef cattle farmers, a trans-logarithmic stochastic frontier function was used to study the relationship between feed utilization efficiency and beef cattle breeding scale. The results showed that the average technical efficiency of feed utilization was 0.56, and the technical efficiency of feed utilization increased year by year. Simultaneously, it showed that Chinese beef cattle farmers pay more attention to feed utilization efficiency at this stage; the feed utilization efficiency of retail and medium-sized beef cattle farmers was in the best state in 2015 and 2016. In 2017, the feed utilization efficiency of small-scale beef cattle farmers was the best; the technical efficiency of beef cattle breeding will increase with the expansion of scale. The technical efficiency of beef cattle breeding will increase with the expansion of the scale, and the feed utilization efficiency of large-scale farmers is also better than that of retail farmers, and the scale of beef cattle breeding can bring better benefits. However, from the perspective of feed utilization efficiency, it is not the largest scale that represents the best efficiency, and from the perspective of breeding technology efficiency, the gap between various scales is gradually narrowing. This should also prove that under the condition of hard resource constraints, the large-scale development of beef cattle breeding is in line with the basic national conditions of China at this stage.

Physiological responses of several beef cattle breeds based on environmental conditions in Beef Cattle Research Station

Physiological conditions were one factors that can affect the growth of cattle. The study aimed to determine the physiological responses of several beef cattle breeds that are reared in Beef Cattle Research Station (BCRS), Grati District, Pasuruan Regency, East Java Indonesia, was carried out by measure temperature, humidity, Temperature Humidity Index (THI), rectal temperature, respiration frequency, body temperature, Head Tolerance Coefficient (HTC) and heart rate of 159 cows consisting of Bali, Madura, Jabres, Galekan, Rambon, Peranakan Ongole (PO), Belgian Blue x PO cross, PO x Bali cross (POBA) which were observed during 4 months in pens. Data were analysed descriptively. The result of the study were ambient temperature and relative humidity of the pen ranged from 25-34.12°C and 54.88-84.27%, THI ranged from 74.92-84.97, body temperature ranged from 35.06-38.53°C, respiration frequency ranged from 20-54 times/minute, HTC ranged from 1.81-3.34, and heart rate ranged from 65-92 times/minute. It was concluded that several breeds of beef cattle in BCRS have mild to severe heat stress, high heat tolerance, respiration rate and heart rate. This condition does not cause production problem in several beef cattle, but in BB x PO cross it has potential to low productivity.

Genetic variation of the candidate loci controlling twinning in the beef and dairy cattle breeds in Indonesia

Multiple births or twinning in cattle is viewed as a natural phenomenon that still needs to be explored in the genetic aspect. The occurrence of twinning birth in cattle has been found in Indonesia both on beef and dairy breeds, however, the frequency is very low. This study aimed to identify the genetic variation of the candidate loci of Bovine chromosome 10 (BTA10) to control twinning in beef and dairy cattle in Indonesia. At least 57 historical twin births of both beef and dairy breeds across Central Java with the highest twins in cattle, were identified in 2017 during the dry season. The ratio of twin calves was relatively similar between female and male calves. Male twins showed the highest number (37.8%), while freemartin heifers were also highly born (28.9%) among twin births. Forty-nine heads out of 152 heads were sequenced on the partial regions in BTA10 that were highly homologous with the ovulation rate gene. DNA sequences polymorphism was identified at three regions in the BTA10 among twins of Simmental, Ongole, Limousin, Simmental-Ongole, and Holstein Friesian cattle. A significant association was not revealed between identified SNP and twinning birth potential in all breeds. Notably, one SNP (A/G) at 410 nucleotides in the consensus sequence of BTA10-2 appeared to be highly significantly associated with twinning birth potential in beef cattle of the Simmental. Taken together, this SNP would be a potential genetic marker for early selection of twinning birth which is promising for dramatic improvement of beef cattle production.

The ATP1A1 Gene Polymorphisms in Indonesian Beef Cattle

In this research, the direct sequencing method was used to identify the polymorphism and genetic diversity of the ATP1A1 gene in Indonesian beef cattle. The study used five breeds of local beef cattle with a total of 60 DNA samples (POGASI = 20, Jabres = 10, Bali = 10, Galekan = 10, and Madura = 10). The amplification of the ATP1A1 gene was using one set of primers (F = 5′- AGG GGT AGC CAG AGT TCC TA – 3′ and R = 5′ – CCC AAA GGT CAC GTG CTT TT – 3′). The result showed 6 SNPs in the APT1A1 gene, namely SNPs g.17293G/A, g.17356C/T, g.17359G/A, g.17541A/G, g.17585 A/G, and g. 17682C/T. Three SNPs were located in coding sequence nine, and the other SNPs were in intron 9 of the ATP1A1 gene. Based on the total population, the Chi-square test indicated that only two polymorphic loci (.17585 A/G and g. 17682C/T) fitted Hardy-Weinberg equilibrium (χ2<5.99). In conclusion, the polymorphic loci of the ATP1A1 gene can be used for genetic diversity and further association study to anti-heat stress traits of Indonesian beef cattle.

Fattening of bulls of Aberdeen-Angus Kalmyk and Charolais breeds in conditions of environmentally safe feeding and keeping

In a scientific and economic experiment on bulls of the Aberdeen-Angus, Kalmyk and Charolais breeds of pasture fattening in the foothill raw material zone of the Labinsk district of the Krasnodar region, the productivity, interior, quality and safety of meat raw materials were studied in a comparative aspect. At the fattening complex of the agricultural company, six-month-old bulls of Aberdeen-Angus, Kalmyk and Charolais breeds were formed into three groups by age according to the principle of analogues (n=12), which were raised from birth using the technology of specialized beef cattle breeding. The first group - Aberdeen-Angus bulls, the second - Kalmyk bulls and the third - Charolais bulls, which had a live weight at the beginning of fattening 189.5 ± 13.8; 175.5±11.5 and 177.1±7.6 kg, respectively. The results of the slaughter of bulls of three breeds revealed a direct relationship between pre-slaughter live weight and slaughter indicators. The pre-slaughter weight of Charolais bulls and, to a lesser extent, Aberdeen-Angus bulls was significantly higher than the mass of Kalmyk bulls. The difference between the slaughter rates of the three breeds of beef bulls is significant in terms of such indicators as slaughter yield, boneless beef yield with the advantage of carcass morphology of Charolais bulls. The slaughter yield was higher in bulls of the Charolais breed compared to groups 1 and 2 by at least 3.2 % and amounted to 69.5 %. In the study of the chemical composition of muscle tissue, a reliably maximum proportion of protein and a minimum proportion of fat in the longissimus muscle of carcasses of Charolais bulls were determined, which amounted to 21.7 and 7.6 %, respectively. A direct positive relationship was determined between meat productivity and the mass fraction of protein in the muscles of bulls of three breeds with such interior indicators as the mass fraction of total protein, protein index. The results of the research allowed to conclude that the biological status of animals has a significant impact on meat productivity, technological properties and the biological value of meat raw materials necessary for the production of baby and functional nutrition.

Comparative analysis of the quality of beef obtained from crossbred steers of beef breeds for the livestock industry development

The scientific article presents the results of the scientific experiment on the study of qualitative indicators of beef obtained from crossbreeding of beef cattle breeds in the conditions of Volgograd agricultural enterprise JSC “Berdievsky elevator”. Obtained crossbred steers at the age of 9 months were divided into 4 groups: I experimental - ½ of a cross (Hereford х Russian Komolaya), II experimental - ½ of a cross (Kazakh Whitehead х Kalmykian), III experimental - ½ of a cross (Kalmykian x Aberdin-Angusian) and IV experimental - ½ of a cross (Aberdin-Angusian x Simmentalian). Formed groups of animals were put on fattening until 16 months of age. Growth dynamics of steers, morphological composition of blood and carcasses, qualitative characteristics of beef were evaluated.

Association of polymorphisms in lipid and energy metabolism-related genes with fattening performance in Simmental cattle

Lipid and energy metabolism are major constituents of mammal growth and thus fattening performance of cattle. This study was designed to evaluate the effects of polymorphisms in lipid and energy metabolism-related genes including oxidized low-density lipoprotein receptor 1 (OLR1), lactoferrin (LTF), stearoyl-CoA desaturase (SCD), beta-lactoglobulin (LGB), thyroglobulin (TG), annexin A9 (ANXA9), myogenic factor 5 (MYF5), protein kinase AMP-activated non-catalytic subunit gamma 3 (PRKAG3), and pituitary-specific transcriptional factor 1 (PIT1), on fattening performance in Simmental cattle. A total of 72 purebred Simmental bulls with a similar initial age and weight were fattened on the same farm for 10 months. Association analysis was performed using linear mixed models. The OLR1 marker was significantly associated with the final weight (FW), hot carcass weight (HCW), chilled carcass weight (CCW), dressing percentage (DP), and total weight gain (TWG). SCD affected the FW, TWG, and average daily live weight gain (ADWG). The present results clearly demonstrated the significant impact of the TG marker on fattening performance. It was highly significantly associated with the FW, HCW, CCW, and TWG. The SCD × TG and the OLR1 × TG interactions had remarkable effects on the traits analyzed. The GACC and CCCC haplotypes of the SCD × TG and OLR1 × TG, respectively, were found to be powerful markers for fattening performance in Simmentals. Novel associations in this study may be useful for further genetic evaluations to improve beef cattle breeding.

BIOLOGICAL VALUE AND TECHNOLOGICAL INDICATORS OF MEAT IN BEEF BULLS OF DIFFERENT GENOTYPES IN THE CONDITIONS OF THE AGRICULTURAL HOLDING "BAISERKE-AGRO"

Annotation The most important biological feature of beef bulls is their ability to consume and process a large amount of cheap low-nutritional feed, including waste from crop production and the food industry, into valuable food products for humans. The meat products and leather raw materials obtained at the same time are of high quality. The development of new directions in breeding, for example, early prediction of the productive qualities of livestock, contributed to a greater opportunity for selection, a reduction in the interval between generations and more effective breeding of cattle in the meat direction of productivity. We need animals that have requirements that provide for obtaining the most "economical animals", combining in their genotype the strength of the constitution and high productivity, good fattening and meat qualities, resistance and immunity to stress, having good adaptive abilities to living conditions and economic use. In other words, it is necessary to create animals with high productivity and well adapted to intensive use. The aim of the research was to improve and optimize breeding methods to increase the rate of genetic progress of different beef breeds of cattle. Based on the interaction of genotypes, to determine effective methods for improving the breeding and productive qualities of meat breeds of different genotypes to increase the production of high-quality beef. This article presents the results of studies of meat productivity of beef bulls of different genotypes in the conditions of the agricultural holding "Baiserke-Agro". According to the results of the research, the quality of carcasses and slaughter indicators, the morphological composition of carcasses and individual anatomical parts, the chemical composition of meat and the energy value of raw fat were established. The conducted studies contributed to the fullest realization of the genetic potential of the productivity of the Aberdeen Angus, Hereford and Kazakh white-headed breeds, as a result, an additional reserve for obtaining high-quality beef was revealed. The proposed methods and techniques of organizing the breeding process allowed to create highly productive beef cattle in the conditions of the agricultural holding "Bayserke-Agro", which contributed to reducing the cost of 1 kg of live weight gain by 5-8% and obtaining additional profit for 1 head by 14-16%. &nbsp; &nbsp

Evaluation of Growth Performance of Grade-2 Brahman Crossbred Progeny in a Farmers’ Participatory Beef Breeding Program

The study was carried out to compare the growth performance of grade-2 Brahman crossbreds to other available cattle genotypes in Bangladesh. Growth traits were birth weight, weight at 3-, 6-, 9-, 12-month of age and average daily weight gain from birth to 12-month of age. The birth, weight at 3-, 6-, 9- and 12-month were 19.87±0.06, 52.01±0.49, 85.03±0.62, 127.12±0.81 and 171.19±1.20 kg, respectively. The average daily weight gain of calves was 426.00±4.99 g. Area and sex had significant effect (p&lt;0.01) on the birth weight, body weight at 3-, 6-, 9- and 12-month of age and average daily weight gain of Brahman calves. Growth performance results on indigenous, Red Chittagong, Pabna, and crosses of Holstein-Frisian, Jersey, Sahiwal and Sindhi cattle were also collected from published literatures, which were compared with 25% Brahman cross calves. Birth weight (19.87±0.87 kg) and yearling or 12-month weight (171.19±17.9 kg) was highest in Brahman cross calves, whereas birth weight (14.81±2.50 kg) was lowest in indigenous calves and yearling weight (92.28±1.73 kg) was in lowest in Sahiwal cross calves. Average daily weight gain was highest (426.00±4.99 g) in Brahman and lowest (146.78±91.96 g) in Indigenous calves. Thus, it may be concluded that growth performance of Brahman cross calves was better than other existing cattle genotypes of Bangladesh. However, further study with large number of samples at different management systems would help to draw a final conclusion. J. of Sci. and Tech. Res. 3(1): 85-94, 2021

1H NMR Metabolic Profiling and Meat Quality in Three Beef Cattle Breeds from Northeastern Thailand

The increasing need for effective analytical tools to evaluate beef quality has prompted the development of new procedures to improve the animal sector's performance. In this study, three beef breeds-Thai native (TN), crossbred Brahman × Thai native (BT), and crossbred Charolais × Brahman (CB)-were compared in terms of their physicochemical and metabolic profiles. The findings demonstrated that TN beef was lighter and tougher than other beef. Beef odor was stronger in BT. In addition, CB beef was the most tender and had the highest intramuscular fat content. Twenty-one different metabolites were found overall through NMR and chemometric approaches. The primary factors contributing to the difference in OPLS-DA loading plots were acetylcholine, valine, adenine, leucine, phosphocreatine, β-hydroxypyruvate, ethanol, adenosine diphosphate, creatine, acetylcholine, and lactate. The multivariate analysis indicated that these metabolites in beef cattle breeds could be distinguished using NMR spectroscopy. The results of this study provide valuable information on the quality and meat metabolites of different breeds. This could help in the development of a more accurate assessment of the quality of beef in future research.

Detection of genetic variation in bovine CRY1 gene and its associations with carcass traits

The biological clock (also known as circadian clock) is closely related to growth and development, metabolism, and diseases in animals. As a part of the circadian clock, the cryptochrome circadian regulator 1 (CRY1) gene is involved in the regulation of biological processes such as osteogenesis, energy metabolism and cell proliferation, however, few studies have been reported on the relationship between this gene and animal carcass traits. Herein, a total of four insertion/deletion (InDel) loci within the CRY1 gene were detected in Shandong Black Cattle Genetic Resource (SDBCGR) population (n = 433). Among them, the P1-6-bp-del locus was polymorphic in population of interest. Moreover, the P1-6-bp-del locus showed two genotypes, with a higher insertion/insertion (II) genotype frequency (0.751) than insertion/deletion (ID) genotype frequency (0.249). Correlation analysis showed that the P1-6-bp-del locus polymorphisms were significantly associated with twenty carcass traits (e.g., slaughter weight, limb weight, and belly meat weight). Individuals with II genotype were significantly better than those with ID genotype for eighteen carcass traits. Therefore, the P1-6-bp-del locus of the CRY1 gene can be used as a molecular marker for beef cattle breeding.

INTERMUNICIPAL AND INTERREGIONAL COOPERATION AND ITS ROLE IN THE DEVELOPMENT OF ETHNIC ENTREPRENEURSHIP IN RURAL AREAS

One of the priorities of modern state policy in relation to rural territories is the development of small and medium-sized enterprises, peasant (farmer) and personal subsidiary farms, agricultural production and consumer cooperation. However, Russian small and medium-sized agribusinesses are now actively developing mainly in the sphere of trade and traditional branches of agriculture: crop production, dairy and beef cattle breeding. Rural entrepreneurship has not yet received proper development in the field of processing agricultural products, forestry, and a number of other branches of the rural economy, including in the field of ethnic business. In the frames of this article, the need for the development of intermunicipal and interregional cooperation in the field of ethnic entrepreneurship of the Republic of Bashkortostan is actualized, which is considered as a "point of growth", one of the promising directions of diversification and clustering of the economy of depressive and subsidized rural areas, increasing incomes and employment of villagers. On the example of specific south- and north-eastern regions of Bashkortostan, the prospects of organizing agricultural production and consumer cooperatives of the second and third levels as a factor of clustering of agro- and ethnic business of a village are analyzed.

The Influence of Altitude of Place on Birth Weight and 3-Month Weight of Bali Cattle on Beef Cattle Breeding Participatory System in Barru Regency

This study aimed to determine the difference in birth weight and 3-month weight of Bali cattle that were reared at different heights. This research was carried out on January 20, 2022, located in Barru Regency. The results showed that Bali cattle at an altitude of &lt;100 masl ( 14.26±0.45a ) differed from birth weight at an altitude of &gt;200 masl (14.00 0.75 b). This indicates that the altitude has a significant effect on cattle birth weight,;the higher the place, the lower the birth weight. The low birth weight of cattle is due to the semi-intensive cattle rearing management ssystem,and the grazing process for cows in lowland areas tends to be better than in highland areas with hilly topography. Based on the body weight of 3 months old Bali cattle from the results of the study the average weight of Bali cattle at an altitude of &lt;100 masl was of 64.86 ± 7.37 kg in average, while at an altitude of 100-200 masl was of 64.06 ± 7.98 kg in average and at an altitude of &gt;200 was of 62.90 ± 7.83 kg in average. This shows that the weight of the cow calf at 3 months’ measurement is not different, and that the place’s altitude has no significant effect on body weight of 3 months old cattle. Artificial insemination (AI) and natural mating methods had a significant effect on birth weight. The average birth weight of calves from cows using the AI method was 16.48 ± 1.27 kg and in natural mating, the average birth weight was 14.08 ±0.63 kg. The average calf weight with the AI mating method was 70.68 4.11 kg, and the average calf weight with the natural mating method was 57.20 3.77 kg, based on the weight of 3-month-old Bali cattle and the results of research with the AI mating method. This is because the growth rate at 3 months of age is still considered fast growth and the weaning period is still dependent on the parent. The conclusion of this study was that the birth weight and 3-month weight of Bali cattle are influenced by different altitudes. The weight of Bali cattle calves at an altitude of &lt;100 masl was significantly different from an altitude of &gt;200 masl. Birth weight and 3-month weight of cattle with the AI mating method were better than the natural mating method