Beckmannia Syzigachne Research Articles

Two Cytochrome P450s, CYP709B1 and CYP704C1, Play Essential Roles in Metabolism-Based Multiple Herbicide Resistance in American Sloughgrass (Beckmannia syzigachne (Steud.) Fernald).

This study confirmed a field population of American sloughgrass (Beckmannia syzigachne (Steud.) Fernald) that developed simultaneously high levels of resistance (resistance index >10) to three divergent modes of action herbicides: fenoxaprop-P-ethyl, mesosulfuron-methyl, and isoproturon. The resistance phenotype observed in this population was not attributed to target-site alterations; rather, the resistant plants exhibited a significant increase in the activity of cytochrome P450s (P450s) and enhanced metabolism rates for all three herbicides. RNA sequencing revealed significant upregulation of two P450s, CYP709B1 and CYP704C1, in the resistant plants both before and after herbicide treatments. Molecular docking predicted that the homology models of these P450s should exhibit a binding affinity for a range of herbicides. The heterologous expression of the identified P450s in yeast cells indicated improved growth in the presence of all three of the aforementioned herbicides. Collectively, the increased expression of CYP709B1 and CYP704C1 likely contributed to the P450s-mediated enhanced metabolism, thereby conferring multiple herbicide resistance in B. syzigachne.

Response of plants and soils to inundation duration and construction of the plant‒soil association mode in the hydro‒fluctuation belt of the reservoir wetland

Hydro-Fluctuation Belt (HFB), a periodically exposed bank area formed by changes in water level fluctuations, is critical for damaging the reservoir wetland landscape and ecological balance. Thus, it is important to explore the mechanism of hydrological conditions on the plant-soil system of the HFB for protection of the reservoir wetland and landscape restoration. Here, we investigated the response of plant community characteristics and soil environment of the HFB of Tonghui River National Wetland Park (China), is a typical reservoir wetland, to the duration of inundation, as well as the correlation between the distribution of dominant plants and soil pH, nutrient contents, and enzyme activity by linear regression and canonical correlation analyses. The results show that as the duration of inundation decreases, the vegetation within the HFB is successional from annual or biennial herbs to perennial herbs and shrubs, with dominant plant species prominent and uneven distribution of species. Soil nutrient contents and enzyme activities of HFB decreased with increasing inundation duration. Dominant species of HFB plant community are related to soil environment, with water content, pH, urease, and available potassium being principle soil environmental factors affecting their distribution. When HFB was inundated for 0–30 days, soil pH was strongly acidic, with available potassium content above 150 mg kg−1 and higher urease activity, distributed with Arundo donax L., Polygonum perfoliatum L., Alternanthera philoxeroides (Mart.) Griseb., and Daucus carota L. communities. When inundated for 30–80 days, soil pH was acidic, with lower available potassium content (50–150 mg kg−1) and urease activity, distributed with Beckmannia syzigachne (Steud.) Fern.+ Polygonum lapathifolium L., Polygonum lapathifolium L., Medicago lupulina L. + Dysphania ambrosioides L. and Leptochloa panicea (Retz.) Ohwi communities. Using the constructed HFB plant-soil correlation model, changes in the wetland soil environment can be quickly judged by the succession of plant dominant species, which provides a simpler method for the monitoring of the soil environment in the reservoir wetland, and is of great significance for the scientific management and reasonable protection of the reservoir-type wetland ecosystem.

Fenoxaprop-P-ethyl, mesosulfuron-ethyl, and isoproturon resistance status in Beckmannia syzigachne from wheat fields across Anhui Province, China

Severe infestations of American sloughgrass (Beckmannia syzigachne (Steud.) Fernald) in wheat fields throughout Anhui Province, China, pose a significant threat to local agricultural production. This study aims to evaluate the susceptibility of 37 B. syzigachne populations collected from diverse wheat fields in Anhui Province to three commonly used herbicides: fenoxaprop-P-ethyl, mesosulfuron-ethyl, and isoproturon. Single-dose testing revealed that out of the 37 populations, 31, 26, and 11 populations had either evolved or were evolving resistance to fenoxaprop-P-ethyl, mesosulfuron-ethyl, and isoproturon, respectively. Among them, 25 populations displayed concurrent resistance to both fenoxaprop-P-ethyl and mesosulfuron-ethyl, while eight exhibited resistance to all three tested herbicides. Whole-plant bioassays confirmed that approximately 84% of the fenoxaprop-P-ethyl-resistant populations manifested high-level resistance (resistance index (RI) ≥10); 62% of the mesosulfuron-ethyl-resistant populations and 82% of the isoproturon-resistant populations exhibited low- to moderate-level resistance (2 ≤ RI <10). Three distinct target-site mutations were identified in 27% of fenoxaprop-P-ethyl-resistant populations, with no known resistance mutations detected in the remaining herbicide-resistant populations. The inhibition of cytochrome P450s (P450s) and/or glutathione S-transferases (GSTs) substantially increased susceptibility in the majority of resistant populations lacking mutations at the herbicide target site. In conclusion, resistance to fenoxaprop-P-ethyl and mesosulfuron-ethyl was widespread in B. syzigachne within Anhui Province's wheat fields, while resistance to isoproturon was rapidly evolving due to its escalating usage. Target-site mutations were present in approximately one-third of fenoxaprop-P-ethyl-resistant populations, and alternative mechanisms involving P450s and/or GSTs could explain the resistance observed in most of the remaining populations.

Double-spraying with different routes significantly improved the performance of both pre- and post-emergence wheat herbicides applied by unmanned aerial spraying systems

Unmanned aerial spraying systems (UASS) are one of the most important systems for herbicide applications. We applied pre-emergence (PRE) and post-emergence (POST) herbicides via an UASS to wheat (Triticum aestivum) fields, using different spraying volumes (22.5–60 L ha−1) and different spraying route strategies (one-time spraying or double spraying with two flight sorties routed in length and breadth). Both the spraying volume and route strategy significantly (P < 0.05) affected the control efficacy against grassy weeds and broadleaf weeds of both PRE and POST herbicides. Compared with manual spraying with a spraying volume of 450 L ha−1, the formulation of PRE herbicides (132 g·ai·ha−1 flufenacet + 132 g·ai·ha−1 diflufenican + 132 g·ai·ha−1 flurtamone) applied by the UASS with a spraying volume of 45 or 60 L ha−1 and adopting the double-spraying technique showed slightly higher or similar control efficacy against grassy and broadleaf weeds. The formulation of POST herbicides (9.75 g·ai·ha−1 florasulam + 9.75 g·ai·ha−1 halauxifen-methyl) showed control efficacies >98% against Galium spurium applied by the UASS with a spraying volume of 22.5–60 L ha−1, according to fresh weights of aboveground parts at 45 d after treatment; however, this was not the case for the control of Stellaria aquatica. The POST herbicides showed the highest control efficacy against S. aquatica when applied with UASS at a spraying volume of 60 L ha−1 and the double-spraying technique. Similarly, another formulation of POST herbicides (15.75 g·ai·ha−1 mesosulfuron-methyl + 60 g·ai·ha−1 pinoxaden) showed similar control efficacies with manual spraying against Beckmannia syzigachne, applied by the UASS with a spraying volume of 45 or 60 L ha−1, using the double-spraying strategy. Conclusion: Applying PRE or POST wheat herbicides with an UASS, double-spraying routed in length and breadth with a spraying volume of 45–60 L ha−1 should be recommended.

Potential of Macrophytes for Wastewater Remediation with Constructed Floating Wetlands in Cold Climates

Nature-based, low technology wastewater treatment systems can benefit small and remote communities. Adding a constructed floating wetland (CFW) to waste stabilization ponds can enhance treatment efficacy at low cost, depending on appropriate macrophytes. In cold climates, harsh growing conditions may limit CFW success, requiring research under-ambient field conditions. Seven native macrophytes were assessed for the growth, biomass production, and root and shoot uptake of potential contaminants of concern from municipal wastewater in a facultative stabilization pond in Alberta, Canada. All macrophytes established. Scirpus microcarpus had high nitrogen and phosphorus in roots and shoots and phytoextraction potential. Metal and trace elements were highest in Glyceria grandis, Beckmannia syzigachne, and Scirpus microcarpus, mostly greater in roots than shoots, indicating phytostabilization. Tissue contaminant concentrations did not always indicate high contaminant accumulation in the CFW. Total uptake per unit area was greatest for Glyceria grandis, although chromium and molybdenum were greatest in Beckmannia syzigachne and Carex aquatilis, respectively. Beckmannia syzigachne and Scirpus microcarpus have potential for phytoremediation if biomass per unit area is increased. Species variability is high for contaminant accumulation and biomass; in unpredictable climates and wastewaters with suites of contaminants, different macrophytes for wetland water treatment systems are recommended.

Metabolic resistance to acetolactate synthase inhibitors in Beckmannia syzigachne: identification of CYP81Q32 and its transcription regulation.

Frequent herbicide use selects for herbicide resistance in weeds. Cytochrome P450s are important detoxification enzymes responsible for herbicide resistance in plants. We identified and characterized a candidate P450 gene (BsCYP81Q32) from the problematic weed Beckmannia syzigachne to test whether it conferred metabolic resistance to the acetolactate synthase-inhibiting herbicides mesosulfuron-methyl, bispyribac-sodium, and pyriminobac-methyl. Transgenic rice overexpressing BsCYP81Q32 was resistant to the three herbicides. Equally, rice overexpressing the rice ortholog gene OsCYP81Q32 was more resistant to mesosulfuron-methyl. Conversely, an OsCYP81Q32 gene knockout generated using CRISPR/Cas9 enhanced mesosulfuron-methyl sensitivity in rice. Overexpression of the BsCYP81Q32 gene resulted in enhanced mesosulfuron-methyl metabolism in transgenic rice seedlings via O-demethylation. The major metabolite, demethylated mesosulfuron-methyl, was chemically synthesized and displayed reduced herbicidal effect in plants. Moreover, a transcription factor (BsTGAL6) was identified and shown to bind a key region in the BsCYP81Q32 promoter for gene activation. Inhibition of BsTGAL6 expression by salicylic acid treatment in B. syzigachne plants reduced BsCYP81Q32 expression and consequently changed the whole plant response to mesosulfuron-methyl. Sequence polymorphisms in an important region of the BsTGAL6 promoter may explain the higher expression of BsTGAL6 in resistant versus susceptible B. syzigachne plants. Collectively, the present study reveals the evolution of an herbicide-metabolizing and resistance-endowing P450 and its transcription regulation in an economically important weedy plant species.

Cytochrome P450 BsCYP99A44 and BsCYP704A177 Confer Metabolic Resistance to ALS Herbicides in Beckmannia syzigachne.

Beckmannia syzigachne is a noxious grassy weed that infests wheat fields in China. Previously, we identified that mesosulfuron-methyl resistance in a B. syzigachne population (R, SD04) was conferred by non-target resistance, such as cytochrome P450 mixed-function oxidases (P450s)-based metabolism. RNA sequencing and real-time PCR (qRT-PCR) were used to discover potential P450s-resistant-related genes. Five cytochrome P450s (CYP704A177, CYP96B84, CYP71D7, CYP93A1, and CYP99A44) were found to be highly expressed in R plants. In this study, CYP99A44 and CYP704A177 were cloned from B. syzigachne and transferred into Arabidopsis thaliana to test the sensitivity of Arabidopsis with and without P450s genes to mesosulfuron-methyl and other acetolactate synthase (ALS)-inhibiting herbicides. Transgenic Arabidopsis overexpressing CYP99A44 became resistant to the sulfonylurea herbicide mesosulfuron-methyl, but showed no resistance to pyroxsulam, imazethapyr, flucarbazone, and bispyribac-sodium. Notably, those overexpressing CYP704A177 showed resistance to pyroxsulam and bispyribac-sodium, but not to mesosulfuron-methyl, imazethapyr, and flucarbazone. These results indicated that B. syzigachne and transgenic Arabidopsis displayed different cross-resistance patterns to ALS-inhibiting herbicides. Subcellular localization revealed that CYP99A44 and CYP704A177 protein were located in the endoplasmic reticulum. Furthermore, these results clearly indicated that CYP99A44-mediated mesosulfuron-methyl resistance in B. syzigachne and CYP704A177 may be involved in B. syzigachne cross-resistance to pyroxsulam and bispyribac-sodium.

A new technique for stain-marking of seeds with safranine to track seed dispersal and seed bank dynamics.

Accurate tracking of seed dispersal is critical for understanding gene flow and seed bank dynamics, and for predicting population distributions and spread. Available seed-tracking techniques are limited due to environmental and safety issues or requirements for expensive and specialized equipment. Furthermore, few techniques can be applied to studies of water-dispersed seeds. Here we introduce a new seed-tracking method using safranine to stain seeds/fruits by immersing in (ex situ) or spraying with (in situ) staining solution. The hue difference value between pre- and post-stained seeds/fruits was compared using the HSV color model to assess the effect of staining. A total of 181 kinds of seeds/fruits out of 233 tested species of farmland weeds, invasive alien herbaceous plants and trees could be effectively stained magenta to red in hue (320–360°) from generally yellowish appearance (30–70°), in which the other 39 ineffectively-stained species were distinguishable by the naked eye from pre-stained seeds. The most effectively stained seeds/fruits were those with fluffy pericarps, episperm, or appendages. Safranine staining was not found to affect seed weight or germination ability regardless of whether seeds were stained ex situ or in situ. For 44 of 48 buried species, the magenta color of stained seeds clearly remained recognizable for more than 5 months after seeds were buried in soil. Tracking experiments using four species (Beckmannia syzigachne, Oryza sativa f. spontanea, Solidago Canadensis, and Acer buergerianum), representing two noxious agricultural weeds, an alien invasive plant, and a tree, respectively, showed that the safranine staining technique can be widely applied for studying plant seed dispersal. Identifying and counting the stained seeds/fruits can be executed by specially complied Python-based program, based on OpenCV library for image processing and Numpy for data handling. From the above results, we conclude that staining with safranine is a cheap, reliable, easily recognized, automatically counted, persistent, environmentally safe, and user–friendly tracking-seed method. This technique may be widely applied to staining most of the seed plant species and the study of seed dispersal in arable land and in disturbed and natural terrestrial or hydrophytic ecological systems.

Sweep Sampling Comparison of Terrestrial Insect Communities Associated with Herbaceous Stratum in the Riparian Zone of the Miho River, Korea.

Simple SummaryInsect pests and their natural enemies can harbor in riparian zones. To determine the impact of insect communities on agriculture and ecology, we must quantitatively assess insect populations in riparian areas. To identify the appropriate methodology for effective insect sampling in riparian areas, we assessed sweep sampling within three plant communities using different numbers of subsampling units (50 sweeps carried out twice, or 10 sweeps over 10 times) over two years. The results reveal that effective insect sampling varies between different plant communities and insect orders. The similarities between terrestrial insect communities in the same plant community were relatively high, even in different years. The optimum sampling size to obtain approximately 80% of the total species was estimated for each survey site. Our results lay the foundations for providing techniques to assess insect populations within riparian areas to predict and prevent herbivorous insect pest invasions in the future.To investigate insect and plant community relationships in riparian zones, terrestrial insect communities were compared in plant communities in the riparian zone of the Miho River, Korea. The sweep netting method was used to sample insects in 50 m transects in three herbaceous plant communities. In 2020, each plant community—Chenopodium album, Beckmannia syzigachne, and Artemisia indica—was swept 100 times (50 sweeps × 2). In 2021, two communities had an additional 100 sweeps collected using 10 subsamples of 10 sweeps (excluding C. album communities). The surveyed dominant species or subdominant species of the insect community in each site preyed on the dominant plant species at the site. The Bray–Curtis similarity was significantly higher than the Sørensen similarity when comparing datasets across different years for the same plant species community. The predicted optimum sampling size to obtain approximately 80% of the total species estimated to be at each survey site, for effective quantitative collection of terrestrial insect herbivores in each plant community, was examined. Fifty sweeps were required for the A. indica community and 100 sweeps were required for the B. syzigachne community. The results of this study provide important data for riparian biodiversity conservation and future pest monitoring.

Pollen-mediated flow of herbicide resistance genes in Beckmannia syzigachne.

Beckmannia syzigachne (Steud.) Fernald has evolved herbicide resistance due to the long-term sole use of herbicides and has become a dominant weed in wheat fields in the middle and lower reaches of the Yangtze River in China. In addition to the selection pressure imposed by herbicides, pollen-mediated gene flow (PMGF) has been reported to cause the spread of herbicide resistance between populations within a certain range in some farmland weeds. It is not clear whether the same is true for the self-pollinated grass weed B. syzigachne. In this study, we confirmed and quantified the level of PMGF in B. syzigachne through concentric circle planting and herbicide resistance tests. Results show that when the B. syzigachne pollen donor was close to the recipient (0.5m), the average gene flow was 0.66%. Gene flow was detected as far as 10m (the farthest distance studied) and decreased exponentially with increasing distance, which could be described by a double exponential decay model. Temperature also affected gene flow, whilst the average level of gene flow in all directions of wind was similar and wind speed caused insignificant difference in gene flow. The results of this study confirmed that PMGF can occur between B. syzigachne populations in adjacent fields. Although the level of resistance spreading by pollen was low, especially across long distance, the results were relevant for smallholding farms, which is the dominant form of agricultural operation in China. It is therefore important to take proactive measures and integrate chemical and ecological weed control methods to prevent the spread of resistant B. syzigachne via both seeds and pollens. © 2022 Society of Chemical Industry.

Влияние микроэлементов на продуктивность многолетних аборигенных трав и качество кормов в условиях крайнего северо-востока России

The improvement of the fodder base of the full nutrition of farm animals is a priority direction of the development of agriculture in the region. Prevention of the process of degradation and reduction of productivity of meadow phytocenoses occurring under the influence of natural and anthropogenic factors determined the relevance of this work. The research was carried out on the basis of the Department of Agroecology, experimental nurseries and the chemical-analytical laboratory of the Magadan Research Institute of Agriculture (Magadan). The aim of the work was to obtain experimental data on the study of the effect of trace elements (Cu, Zn, B) on the productivity of meadow agroecosystems and the quality of forage phytocenoses. The effect of the combined use of macro- and microelements on the productivity of old-age crops of Eastern beckmania (Beckmannia syzigachne (Steudal) Fernald) and reed foxtail (Alopecurus arundinaceus Poire) and feed quality was studied. According to the results of the experiment, the introduction of copper against the background of a full mineral fertilizer had a positive effect on the yield of the eastern bekmania in the year of application, increasing the hay yield by 2.7 times and the protein yield per hectare by 3.8 times. Foliar fertilizing with boron and zinc in the year of application with a lack of moisture sharply increased the yield of crops of moisture-loving eastern bekmania by 7.9 times. The productivity and quality of the reed foxtail grass has been increased with the addition of copper (37.6%) and zinc (44.9%). The greatest increase in the protein content in foxtail hay was provided by the introduction of boron, in the year of fertilizing the herbage – 14.2%, in the aftereffect - 17.4%. Under the influence of copper, the content of carotene in the dry matter of the eastern bekmania hay significantly increased – in the year of application – by 124%, in the aftereffect – by 43%; and fat – by 15% and 8%, respectively, the calcium content increased significantly: by 25% compared to the control.

Overexpression of a Glycosyltransferase Gene from a Metabolically Poly-Resistant Beckmannia syzigachne Population Alters Growth and Confers Herbicide Resistance to Brachypodium distachyon

Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China. The B. syzigachne (AH-02) population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl. To investigate the function of GT73C1 in this population, the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction, and the sequence was 100% consistent with the transcriptome data. Its phylogenetic tree was displayed and annotated using FigTree v1.4.4. The plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon plants. Furthermore, the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl, which was consistent with the findings from the whole plant bioassay. These results indicate that GT73C1 is closely related to the metabolic resistance of B. distachyon.

Resistance to mesosulfuron-methyl in Beckmannia syzigachne may involve ROS burst and non-target-site resistance mechanisms

Herbicide resistance to chemical herbicide is a global issue that presents an ongoing threat to grain production. Though it has been frequently implicated that the production of detoxification enzymes increased in resistance development, the mechanisms for overexpression of these genes employed by herbicide-resistant weeds remain complicated. In this study, a mesosulfuron-methyl resistant Beckmannia syzigachne population (R) was found to be cross-resistant to another herbicide pyriminobac-methyl. No known target-site mutations were detected in the R population. In contrast, the decreased uptake and enhanced metabolic rates of mesosulfuron-methyl were detected in the R than the susceptible (S) population. Two candidate ATP-binding cassette (ABC) transporter genes (ABCB25 and ABCC14) that were constitutively up-regulated in the R population were identified by RNA-sequencing and validated by RT-qPCR. Alteration of antioxidant enzyme activities and gene expressions implied that mesosulfuron-methyl-induced antioxidant defenses provoked reactive oxygen species (ROS) burst. ROS scavenger assay showed that ROS induces ABCB25 and ABCC14 expression. This study reported for the first time that ABC transporters mediated non-target-site resistance contributes to mesosulfuron-methyl resistance in a B. syzigachne population, and implicated that ROS burst might be involved in the overexpression of ABC transporter genes in weeds.

ПОЛИПЛОИДИЯ У ВОДНЫХ И ПРИБРЕЖНО-ВОДНЫХ СОСУДИСТЫХ РАСТЕНИЙ СЕВЕРО-ВОСТОКА АЗИИ

The chromosome numbers of aquatic and semiaquatic vascular plants growing in extreme North-East Asia, east of the Lena River, were analyzed. We have reviewed the information about the karyological peculiarity of the aquatic flora based on published data, including our own definitions. The karyological data are considered depending on the taxonomic position, distribution and ecological characteristics of the species. The chromosome numbers are known for 119 out of 123 species. There is no data on Zannichellia komarovii, Eleocharis termale, Stuckenia subretusa and Potamogeton sibiricus. In the aquatic flora of North-East Asia, the proportion of polyploids is higher (79%) than that reported for the entire Beringian flora (69%). We analyzed species for the presence of polyploidy depending on the type of area. Analysis by latitudinal groups revealed that all Arctic and hypoarctic species of aquatic plants are polyploids. The minimum proportion of polyploids is observed among arctoboreal species. Analysis by longitudinal groups revealed that the lowest proportion of polyploids was noted among species found only in Eurasia and absent in North America (61%), and it was the highest among pluriregional species (96%). The species of the families Cyperaceae, Lemnaceae, Ranunculaceae and Poaceae are characterized by a high level of variability in the number of chromosomes. The greatest variety of chromosome numbers is observed in the polymorphic species Caltha palustris s. l., Agrostis stolonifera, Dupontia fisheri s. l., Phragmites australis, in all species of the genus Eleocharis, and in Nymphaea tetragona, Acorus calamus, Calla palustris. The isolated cases of a high level of ploidy were found in the extreme northeast, at the northern borders of ranges, in 3 species - Hippuris vulgaris, Arctophila fulva and Beckmannia syzigachne. Only diploids are known in the families Equisetaceae, Typhaceae, Zosteraceae from the flora of North East Asia. We revealed the existence of several chromosomal races in Ranunculus gmelinii and Caltha palustris (Ranunculaceae) in the Magadan region; the distribution of chromosomal races is partly related to geographic location and partly to plant habitats. Rare karyotypes in these species are observed in aquatic forms with mainly vegetative reproduction, growing in non-freezing watercourses.

Effect of an adjuvant, Jijian, on isoproturon to control fenoxaprop-P-ethyl-resistant Beckmannia syzigachne

Continuous and exclusive use of fenoxaprop-P-ethyl has resulted in serious resistance in Beckmannia syzigachne. Isoproturon, a photosystem II inhibitor, is very frequently used in wheat fields, and there are currently few reports about isoproturon resistance in B. syzigachne. However, excessive use of isoproturon is detrimental to the ecological environment. Therefore, adding additives is a good choice to enhance the effect of isoproturon and reduce its usage. Jijian is a non-ionic and polyhydric alcohol surfactant. Greenhouse whole-plant bioassay results showed that 0.05% (v/v) of Jijian + 600 g ai ha−1 of isoproturon achieved the best synergistic effect on controlling fenoxaprop-P-ethyl-resistant B. syzigachne. Jijian significantly reduced the surface tension and contact angle of the isoproturon solution by 15.8 mN m−1 and 33.2°, respectively, and significantly increased the expanded diameter and maximum retention by 0.92 mm and 8.39 mg cm−2 respectively. In conclusion, Jijian had a synergistic effect on isoproturon in the control of fenoxaprop-P-ethyl-resistant B. syzigachne and Jijian may achieve the synergistic effect by changing the physical properties of isoproturon solution on weeds.

Intra- and cross-field dispersal of Beckmannia syzigachne seed by a combine harvester.

Beckmannia syzigachne (Steud.) Fernald has become a dominant weed that has evolved resistance to major herbicides used in the wheat fields of rice-wheat double cropping areas of the middle and lower reaches of the Yangtze River, China. Seed dispersal occurs over long distances via irrigation water. As mechanical harvesting services popularize, there is concern that combine harvesters could play an increasing role in B. syzigachne seed dispersal. Random sampling of 30 combine harvesters at wheat harvest determined that an average of 8000 B. syzigachne seeds remain in the combine after wheat harvesting, predominantly on the metal plate. These seeds could potentially be transported into adjacent fields. A double exponential model predicted that seeds remaining on the metal plate could be dispersed over 7885 m2 into the next field. Within a field, the number of fallen seeds and their dispersal distance were positively correlated to panicle density. Combines spread seeds away from the source potentially creating new weed patches. During irrigation and rotary tillage ploughing, 70% of B. syzigachne seeds scattered in the field floated on the water surface and were moved away by the wind. Both wheat combine harvesters and water flow effectively spread B. syzigachne seeds. Areas with high B. syzigachne population density should be carefully harvested separately, and the metal plate should be carefully cleaned to prevent spreading the weed across fields and region. Floating B. syzigachne seeds displaced to field edges by water can be physically removed with nets to prevent further distribution by water.

Modeling the Effects of Nitrogen Fertilizer and Multiple Weed Interference on Soybean Yield

Understanding the effects of nitrogen (N) fertilizer on soybean-weed competition is essential for establishing a practical tool for N application and weed management. A two-year field experiment was conducted in a soybean field located in Bogatyrka (43.82° N, 131.6° E), Primorsky krai, Russia, to investigate the effects of N fertilizer and multiple-weed interference on soybean (Glycine max) yield and to model these effects. Soybean yield loss caused by the interference of multiple weeds including common ragweed (Ambrosia artemisiifolia), barnyard grass (Echinochloa crus-galli), and American slough grass (Beckmannia syzigachne) at different levels of N fertilizer was accurately described by a combined model incorporating inverse quadratic and exponential models into the rectangular hyperbolic model for two parameters Y0 and β, respectively. The combined model used in our study indicated that the application of N up to 36 kg N ha−1 can increase weed-free soybean yield by 2.2 Mg ha−1 but soybean yield under multiple-weed interference can sharply decrease with increasing total density equivalent, particularly at 36 kg N ha−1. These results, including the combined model, thus can support decision making for weed management under different N uses in soybean cultivation.

The Ile-2041-Val mutation in the ACCase gene confers resistance to clodinafop-propargyl in American sloughgrass (Beckmannia syzigachne Steud).

Exploring the mechanisms of herbicide resistance in weeds is an important part of designing resistance management strategies and rationalizing herbicide use. Beckmannia syzigachne is one of the most important agricultural weeds in China. Long-term use of acetyl-CoA carboxylase (ACCase)-inhibiting herbicides has led to the evolution of herbicide resistance in B. syzigachne. ACCase-inhibiting herbicides comprise three chemical families: aryloxyphenoxypropionates (APPs), cyclohexanediones (CHDs) and phenylpyraxoline (DENs). Based on whole-plant dose-response experiments, a B. syzigachne population (BS-R) was confirmed to be 12- and 20-fold resistant to the APP herbicides quizalofop-P-ethyl and clodinafop-propargyl, and 2.2-, 2.8- and 2.8-fold resistant to fenoxaprop-P-ethyl, the CHD herbicide sethoxydim and the PPZ herbicide pinoxaden, respectively, compared with its susceptible counterpart (BS-S). Resistance to clodinafop-propargyl in the BS-R population could not be reversed by the known cytochrome P450 (CYP450) inhibitor malathion and the glutathione S-transferase (GST) inhibitor 4-chloro-7-nitrobenzoxadiazole. In addition, no difference in CYP450 and GST activity was confirmed between the BS-R and BS-S populations. ACCase gene sequencing revealed an Ile-2041-Val mutation in the BS-R population. A derived cleaved amplified polymorphic sequence marker was developed for rapid detection of the specific Ile-2041-Val mutation. Correlation quantification of resistance in homo- and hetero-resistant versus wild-type plants showed that resistance to clodinafop-propargyl in this population is conferred by the Ile-2041-Val mutation. Unlike previous reports on the unique cross-resistance pattern conferred by the 2041 mutation, this study demonstrates that the Ile-2041-Val mutation in BS-R population confers resistance to certain ACCase-inhibiting APP, CHD and PPZ herbicides in B. syzigachne. © 2021 Society of Chemical Industry.

Changes in dominant weeds of wheat in a rice–wheat rotation system as affected by composted manure and straw amendments

AbstractA study was conducted to identify whether composted manure and straw amendments (replacement of a portion of chemical fertilizer [50% of the total nitrogen application] with composted pig manure, and straw return [all straw from the previous rice crop] combined with chemical fertilizer) compared with no fertilization and chemical fertilizer only would change the dominant species of wheat-associated weeds as well as influence their growth and seed yield in a rice (Oryza sativaL.)–wheat (Triticum aestivumL.) rotation system. The study was initiated in 2010, and the treatment effects on the species, density, plant height, shoot biomass, seed yield of dominant weeds, and wheat yields were assessed in 2017 and 2018. Fertilization significantly increased the height, density, and yield of wheat, as well as the shoot biomass of wheat-associated weeds, but decreased the weed species number. A total of 17 and 14 weed species were recorded in the experimental wheat fields in 2017 and 2018, respectively. The most dominant weed species were American sloughgrass [Beckmannia syzigachne(Steud.) Fernald] and catchweed bedstraw (Galium aparineL.), which made up more than 64% of the weed community in all treatments. When the chemical fertilizer application was amended with pig manure compost and straw return, the relative abundance ofB. syzigachnesignificantly decreased, while the relative abundance ofG. aparinesignificantly increased. The application of the chemical fertilizer-only treatment resulted in increases in the density, shoot biomass, and seed yield ofB. syzigachne, while the composted manure and straw amendments applied together with chemical fertilizer led to significant increases in the density, shoot biomass, and seed yield ofG. aparine. Consequently, further research on ways to promote greater cropping system diversity will be needed to prevent the selection of weed species that are adapted to a limited suite of crop management practices.

RNA-Seq transcriptome analysis to identify candidate genes involved in non-target site-based mesosulfuron-methyl resistance in Beckmannia syzigachne

American sloughgrass (Beckmannia syzigachne Steud.) has become a dominant weed in fields with rice-wheat rotation. Moreover, herbicide resistance has rendered weed control difficult. We identified a biotype showing resistance to ALS inhibitor mesosulfuron-methyl with a resistant index 3.3, but without any ALS mutation. This study aims to identify and confirm the factors associated with non-target site resistance of this biotype to mesosulfuron-methyl using RNA-Seq. 118,111 unigenes were assembled, and 50.9% of these were annotated across seven databases. Eleven contigs related to metabolic resistance were identified based on differential expression via RNA-Seq which include a novel resistance-related transcription factor (MYC3) and two disease resistance proteins were also identified (At1g58602 and At1g15890). Fold changes in expression of these genes in comparison M-R vs. M-S ranged from 3.9 to 11.6, as confirmed by qPCR. The expression of a contig annotated as cytochrome P450 (CYP86B1) in resistant individuals was over 3 times higher than that in sensitive individuals at 0–72 h after mesosulfuron-methyl treatment. A similar trend was noted for three other genes annotated as glutathione S-transferase (GST), namely GST-T3, GST-U6, and GST-U14; the expression of GST-U6 in resistant individuals was up to 142.3 times higher than that in sensitive individuals at 24 h after mesosulfuron-methyl treatment. In addition, GST activity in resistant individuals was 2.1 to 5.3 times higher than that in sensitive individuals. The GR50 of resistant biotype decreased from 24.4 to 11.3 g a.i. ha−1 after P450 inhibitor malathion treatment. This study identified a cytochrome P450 gene CYP86B1 and three GST genes GST-T3, GST-U6, and GST-U14 that have higher expression in mesosulfuron-methyl resistant B. syzigachne, suggesting that both P450- and GST-based activities could be involved in resistance.