Beany Odor Research Articles

Fermentation of Texturized Pea Protein in Combination with Proteases for Aroma Development in Meat Analogues.

The potential use of texturized pea protein in meat analogues was investigated by comparing the effects of fermentation on pea and myofibrillar pork proteins in a model system including additives, microbial starters, and proteases. Model fermentation was controlled for 15 days by a pH decrease and microbial count and free amino acid increase. Besides, volatile production and sensory properties were evaluated at the end of fermentation. Protein type affected free amino acid generation and volatile profile. Models supplemented with proteases showed an increase in amino-acid-derived compounds (branched aldehydes and alcohols) and fruity odor notes. During fermentation, protease addition significantly reduced the production of linear aldehydes (pentanal, hexanal, and octanal) in vegetal models, while pyrazine compounds were not affected. This changes in the volatile profile reduced the legume beany odor but increased the perception of toasted cereal-like notes generated by the texturization process.

Effects of soybean endogenous enzyme hydrolysis on the quality of soymilk after blanching

This study examined the relationship between the endogenous enzymatic hydrolysis conditions and the quality and flavor of soymilk derived from blanched soybeans. Results represented that following blanching, the lipoxygenase activity of soybeans decreased by 88.50% and the endogenous protease activity greatly improved compared to unblanched. During the subsequent endogenous enzymatic hydrolysis, the stability factor and sensory score dropped as the amount of okara rose, although the content of peptides and amino acids nitrogen increased. At an enzymolysis temperature of 50 °C, the soymilk had the highest sensory score. After 60 min of enzymolysis, the beany odor was weakened maximumly; the levels of polypeptide and amino acid nitrogen increased by 45.00% and 43.33%, respectively. Enzymatic hydrolysis at 50 °C for 60 min without okara generated the finest soymilk. Blanching diminished the primary components of the beany odor of raw soymilk. Enzymolysis of blanched pretreatment soymilk further dropped the content of beany odor components and exhibited a richer variety and concentration of volatile components. Following the hydrolysis of endogenous enzymes generated after blanching, the overall quality of soymilk was enhanced.

Effect of l-cysteine on functional properties and fibrous structure formation of 3D-printed meat analogs from plant-based proteins

The development of three-dimensional (3D) printed meat analogs with fiber, texture, and sensory resembling meat remains challenging. This study investigated the effect of l-cysteine on functionality enhancement and fibrous structure formation in mixtures of mung bean protein isolate (MBPI) and wheat gluten (WG) for meat analog production. 3D printing was used to construct fibrous filaments. Raw MBPI-WG mixtures decreased rheological properties when increasing l-cysteine contents (0.0%–0.6%), promoting ink extrudability. The cys-0.4% ink exhibited the highest printing resolution and structural stability, correlated with its higher mechanical strength and increased disulfide cross-links. After cooking, the cys-0.4% sample showed a pronounced fibrousness in agreement with its microstructure image. This meat analog displayed a muscle-meat-like structure, improved texture, and reduced beany odor and bitter taste. Excessive cysteine contents (0.5%–0.6%) negatively affected the functionality of meat analogs. This study provides guidance for optimizing the amount of l-cysteine in meat analogs to improve product quality.

Effect of defatted rice bran addition on properties of texturized soy and rice protein products

Abstract The objective of this work was to produce low moisture-texturized vegetable protein (LM-TVP). Firstly, SPI:DRB at ratio of 50:50, 65:35 and 80:20 (w/w) was blended to produce TVP with 50–70 % protein (TSP50–70), respectively. In addition, RPI:DRB was also blended at the same ratio to produce non beany odor TVP with 50–70 % protein (TRP50–70). All samples were extruded using twin screw extruder. Extrusion condition was fixed at 25 % of feed moisture, 400 rpm of screw speed and 130 °C of die temperature. Next, properties of TSP50–70 and TRP50–70 before and after rehydration were characterized. For TSP50–70, studied properties consisting of expansion ratio, density, WAC, WHC and OHC of TSP50–70 were 2.20–2.27 %, 0.26–0.34 kg/m3, 414–446 %, 2.87–4.88 g H2O/g and 2.55–2.73 g oil/g respectively. These properties values were closed and in the same range of commercial-TVP (C-TVP). However, texture of TSP50 and TSP70 was significantly different, as decreasing DRB content from 50 (TSP50) to 20 % (TSP70) improved texture in terms of hardness (from 0.51 to 1.88 N), springiness (from 0.73 to 0.96) and cohesiveness (from 0.46 to 0.81) of TSP. Therefore, to add value to DRB, TSP70 (80 % SPI and 20 % DRB) was proposed to produce TSP. For TRP, expansion ratio, density, WAC, hardness, springiness and cohesiveness of TRP50–70 were 1.07–1.34 %, 0.63–0.88 kg/m3, 78–158 %, 1.03–9.10 N, 0.71–0.85 and 0.27–0.29 which were out of range compared to C-TVP.

Effect of co-fermentation system with isolated new yeasts on soymilk: microbiological, physicochemical, rheological, aromatic, and sensory characterizations.

The beany flavor adversely influences consumer acceptance of soymilk (SM) products. Thus, in this work, the co-fermentation of isolated new yeasts (Kluyveromyces marxianus SP-1, Candida ethanolica ATW-1, and Pichia amenthionina Y) and Kluyveromyces marxianus K (a commercial yeast) along with an XPL-1 starter (including five strains of lactic acid bacteria (LAB)) was utilized to mend the beany flavor of fermented SM (FSM) beverages. Probiotic count, pH, titratable acidity, syneresis, water holding capacity, rheological characteristics, and sensory attributes were investigated. Furthermore, the free amino acids, nucleotides, and volatile compounds (VCs) were analyzed, also presenting the collected VC data by exploiting a principal component analysis (PCA) and a heatmap with a hierarchical cluster analysis. The co-fermentation with Kluyveromyces marxianus SP-1 and K remarkably enhanced the LAB strain growth and acid production, improving the rheological attributes, whereas that of yeast along with XPL-1 as a mullite starter could reduce the beany odor. PCA chart displayed that higher amounts of alcohols, ketones, acids, and esters that significantly improved the flavor quality of FSM beverages were generated throughout the co-fermentation process. The co-fermentation with Pichia amenthionina Y generated the highest acetoin (36.19%) and diacetyl (2.02%), thus improving the overall acceptance of FSM, as well as the sensory characteristics of FSM beverages with the highest umami, sweet, odorless amino acids, and umami nucleotides, and the lowest content of alcohol and inosine. Taken together, the co-fermentation of Pichia amenthionina Y along with XPL-1 within SM provides novel insights regarding the development of FSM and fermented beverages.

Statistical evaluation to validate matrix-matched calibration for standardized beany odor compound quantitation in yellow pea flour using HS-SPME-GC-MS.

Accurate and precise quantitation of beany odor compounds is important in developing yellow pea (Pisum sativum L., YP) flour-based foods. Aiming at establishing standardized external calibration using an internal standard (ECIS) quantitation method, the effect of solvent extraction on matrix deodorization and systematic statistical analysis on quantitation was evaluated. Initially, accelerated dichloromethane extraction on YP flour and starch produced two clearest deodorized matrix-matched matrices. Secondly, due to the heteroskedasticity, weighted least squares regression (WLSR) was introduced to build calibration curves. The curve linearity and regression parameters were further confirmed via a t-test. Lastly, methodology indicators including LOD/LOQ, accuracy and precision, and the matrix effect (ME) were assessed. Results showed that there were no significant differences in the quantity of beany odor compounds interpolated from two deodorized matrices. This study demonstrated for the first time that despite the unignorable ME, deodorized starch is a feasible and affordable alternative to deodorized YP flour in the quantitation of beany odor compounds to achieve a reliable result.

Reduction of aldehydes with green odor in soy products during fermentation with Lycoperdon pyriforme and analysis of their degradation products

Soy products are rejected by Western consumers because of their green and beany off-aroma. The off-aroma profiles of typical soy products (soy drink and soy protein isolate) were analyzed and forty-five regions imparting green and beany odor impressions were perceived after application of direct immersion-stir bar sorptive extraction coupled with gas chromatography-mass spectrometry-olfactometry. After a fermentation of 28 h with Lycoperdon pyriforme the intensity of the off-aroma was reduced. Accordingly, thirteen and fourteen of the thirty-five quantified compounds were significantly decreased after fermentation of soy drink and soy protein isolate, respectively. The major off-aroma contributors, aldehydes, were reduced during fermentation and their degradation products were analyzed. The following bio-pathways were suggested (i) saturated aldehydes were reduced to the corresponding alcohols with increased odor threshold, leading to less intense sensorial perception, and (ii) di-unsaturated aldehydes did not form any odor-active substance in the volatile spectrum. Both of the phenomena explained the decreased sensorial perception of the off-aroma.

Combining solid dispersion-based spray drying with cyclodextrin to improve the functionality and mitigate the beany odor of pea protein isolate

The beany flavor of pea protein limits its application in the food industry. This study aimed at addressing this problem by combining the advantages of solid-based spray drying technique and the ability of cyclodextrins (CD) to entrap volatiles. Pea protein isolates (PPI) was extracted by alkaline extraction-isoelectric precipitation, followed by co-spray drying with CD. The resulted PPI-CD showed no major structure changes. HS-SPME-GC–MS coupled to untargeted metabolomics successfully identified 23 aroma compounds that represent the different odorants among PPI-control, physically mixed PPI-CD, and co-spray dried PPI-CD samples. Heat map analysis also showed a remarkable beany odor mitigation effect upon the addition of CD, which was further proved to be due to CD entrapping aroma compounds during spray drying. In the meantime, the functional attributes of PPI-CD were not adversely impacted by the addition of CD.

GC-MS olfactometric characterization of odor active compounds in cooked red kidney beans (Phaseolus vulgaris)

Red kidney beans are a staple pulse crop well known for its unique flavor characterized by kidney bean like, smoky, sulfury and earthy aroma notes. However, nature of compounds responsible for the unique beany odor of the cooked pulse has not been established. Steam distillation extracts of red kidney beans were subjected to Gas-chromatography olfactometry (GC-O) techniques namely detection frequency and aroma extract dilution analysis (AEDA). GC-O results suggest that methional with flavor dilution (FD) factor 21 is responsible for imparting the characteristic odor of the cooked red kidney beans. Apart from this p-vinyl guaiacol (FD 13) was identified as most important contributor towards smoky odor note. Sulfury note was mainly contributed by diethyl sulfide (FD 10) while 2-ethyl-3-methyl pyrazine (FD 13) was identified to be responsible for earthy note in cooked red kidney beans. Contribution of these compounds in characteristic aroma of cooked red kidney beans is reported here for the first time.

Soybean residue (okara) fermentation with the yeast Kluyveromyces marxianus

The soybean residue (okara) with high moisture content has generally been discarded, which has led to economic loss and socio-environmental problems. The effects of solid state fermentation using Kluyveromyces marxianus on the nutritional components, beany odor and processing properties of soybean residue were studied. The results showed that K. marxianus fermented soybean residue (FSR) had an increase in crude fat (24.5%), soluble dietary fibers (158%) and polysaccharides (262%), while phytic acid (61.7%) and the activity of a trypsin inhibitor (92.7%) decreased. A significant reduction in the beany odor was also observed. FSR showed higher water absorption and better emulsifying properties. Overall, the study indicated that fermentation with K. marxianus resulted in improved nutritional values and processing characteristics of the soybean residue.

Antioxidant and Hypocholesterolemic Properties of Functional Soy Yogurts Fortified with ω‐3 and ω‐6 Polyunsaturated Fatty Acids in Balb/c Mice

Increase of ω3 and ω6 polyunsaturated fatty acids (PUFA) intake is currently recommended. Yogurt, which is already popular, offers a functional food matrix to deliver ω3 and ω6 PUFAs. The aim of this study is to investigate the antioxidative and cholesterol‐lowering properties of functional soy yogurts fortified with fish oil (FO) containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and Evening primrose oil (EPO) containing gamma linolenic acid (GLA) in diet induced hypercholesterolemic mice. Adding FO and EPO results in a significant reduction in the serum total cholesterol (TC), triglycerides (TG) and low–density lipoprotein cholesterol (LDL‐C), and the atherogenic index of hypercholesterolemic mice when compared with soy yogurt (control) only. Soy yogurts supplemented with functional lipids had significantly improved the high density lipoprotein cholesterol (HDL‐C) in serum, super oxide dismutase and catalase level in liver homogenate and significantly declined the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and gamma glutamyl transferase activities.Practical Applications: FO and EPO may be considered as novel functional food ingredients for soy yogurt production. The process is commercially economical in view of the uses of seed flour as direct raw material without beany odor problem for making non‐dairy food product.Consumption of poly unsaturated fatty acid fortified soy yogurt results in a significant reduction in the liver total cholesterol of hypercholesterolemic mice when compared with soy yogurt (control) only.

Effects of water absorption of soybean seed on the quality of soymilk and the release of flavor compounds.

The water absorption of soybeans during soaking is directly related to the quality characteristics and the flavor properties of soybeans for processing. In this paper, the effects of water absorption of soybean seed on the quality of soymilk and the release of flavor compounds were investigated during soaking at 4 °C, 25 °C, and 50 °C at different pH values. The results showed that the water absorption rate increased as the soaking temperature and pH increased, while the equilibrium value was relatively stable. Peleg's equation with good fitting of the absorption kinetics was used to predict the hydration characteristics of undehulled soybean. MALDI-TOF/TOF-MS results showed that the major released proteins are basic 7S globulin, which is released in large amounts at high temperature. The water absorption of soybean seed significantly enhanced the extraction yields of protein, fat and solids of the prepared soymilk, and alkaline soaking pH further promoted the extraction of proteins and solids. A high soaking temperature can significantly decrease the required soaking time; however, it is unfavorable to the extraction yields of fat, proteins and solids, as well as the whiteness values and the particle sizes. The beany odor compounds of soymilk mainly consisted of hexanal, trans-2-hexenal, 1-octene-3-ol, hexanol, and 2-pentylfuran, and their contents were positively correlated with soaking temperature. A good balance of soymilk quality and flavor compound release can be achieved with soaking conditions of 25 °C and pH 9.

ผลของสภาวะการอบต่อการคงอยู่ของกลิ่นถั่วและสมบัติทางเคมีของแป้งถั่วดาวอินคา

ผลของสภาวะการอบต่อการคงอยู่ของกลิ่นถั่วและสมบัติทางเคมีของแป้งถั่วดาวอินคา

Aroma characteristics of lupin and soybean after germination and effect of fermentation on lupin aroma

Greater human consumption of Australian sweet lupin (Lupinus angustifolius) and other legume products such as soybean (Glycine max) is often limited due to undesirable beany flavor. The impacts of germination of lupin and soybean seeds and fermentation of lupin flour on aroma profiles were compared by gas chromatography olfactometry using trained sensory assessors. Untreated soybean compared to untreated lupin had significantly higher concentrations of volatiles commonly associated with beany flavor in legumes; (E)-2-hexanal, (E)-2-octenal, 1-octen-3-one, 1-hexanol and 1-heptanol. After germination of lupin and soybean, 2-methylbutanal was the most abundant volatile, increasing meaty and brothy odor characteristics. Germination of lupin and soybean also increased sweet, woody, mushroom and baked aroma notes. Fermentation of lupin increased mushroom, soil, green and nutty aroma characteristics, however beany odor did not decrease. Germination and to a lesser extent fermentation, may be successful strategies to increase the acceptability of legume flavor.

콩나물 식미 결정 성분 요인 분석

Soybean sprout is a year-round traditional vegetable that is easily produced and relatively inexpensive in Korea. In addition, the sprout is known as a good source of protein, vitamins and minerals. The quality of the soybean sprout has been mainly evaluated only by its appearance like length, width, color, and the others without considering any odor or taste attributes. We studied the chemical factors affecting taste of soy sprouts cultivated with 5 recommended soybean cultivars through evaluation of chemical constituents in relation to their sensory characteristics. Correlation coefficient among the chemical constituents and sensory characteristics of soybean sprout showed that the linolenic acid and Ca contents were positively correlated with total acceptability of soybean sprout and histidine, aspartic acid, and serine showed a negative association with beany odor of soybean sprout. Multiple regression analysis was done to formulate selection criteria for good taste of soy sprout. The estimation of step-wise regression analysis conducted by 47 chemical components for major quality-related characteristics showed that linolenic acid and mineral contents were the main components increasing the acceptability of soybean sprout.

Effect of Green Gram Semolina (P haseolus aureus ) on the Rheology, Nutrition, Microstructure and Quality Characteristics of High-Protein Pasta

Split green gram (Phaseolus aureus) was roller milled into semolina and was substituted at 20, 40, 60 and 80% levels in vermicelli making in this study. The ash and protein content increased in blends as the level of green gram semolina (GGS) increased. The quality characteristics of pasta showed marginal increase in cooking loss up to 60% level of GGS. The firmness value increased from 36.20 to 36.80 N with 20–60% GGS in pasta. At 80% level of GGS, the cooking loss and stickiness value were highest (7.8% and 0.80 N), whereas firmness value and overall quality score were lowest (32 N and 27.5/40.0), indicating that the pasta had mushy, indiscrete, sticky strands and had a prominent beany odor making it unacceptable. Hence, 60% GGS was considered optimum in vermicelli. The pasta made with 60% GGS had a protein and dietary fiber contents of 16 and 6%, respectively, with in vitro protein digestibility value of 62%. PRACTICAL APPLICATIONS In India, pasta is becoming very popular and the consumption is increasing. Pasta is generally made from durum semolina, and the protein content is generally around 10–11%. To enrich the nutritive value of pasta, many ingredients are added out of which protein sources occupy an important place. Legumes are added in the powdered form to make high-protein pasta. This study aims at using roller mill for producing semolina from legume and its utilization in high-protein pasta. This offers lot of convenience to pasta industries as legume semolina can be made in large quantity.

설렁탕 육수의 이화학적 특성과 기호인자 간의 상관성에 관한 연구

Seolleongtang, a traditional broth in Korea, is expected to become a hot global item for its unique flavor and healthiness. In this study, the correlations between sensory attributes and physicochemical characteristics were studied for the popular seolleongtang stock products through descriptive analysis, sensory evaluation and analysis of salt, soluble solid, protein, viscosity and color for the quality control. Our results indicate that color, concentration, viscosity, and freshness are important attributes in evaluating the quality of seolleongtang stock. There were significant differences between preference and sensory attributes among the stock products. Significant correlations were found between flavor and texture and overall acceptability, as well as beany odor and aroma. Preference and physicochemical aspects also correlated, and indicate that a range of salt, solubility, and viscosity are applicable as quality control factors in seollengtang stock.

Influence of Blanching and Grinding Process with Hot Water on Beany and Non‐Beany Flavor in Soymilk

A total of 8 beany odor-active compounds and 4 non-beany aroma-active compounds of traditional soymilk were identified through dynamic headspace dilution analysis (DHDA) and gas chromatography-olfactometry-mass spectrometry (GC-O-MS). To eliminate the beany flavors, soymilk was processed with hot water blanching and grinding for 2, 4, 6, 8, and 10 min with a temperature between 80 and 100 °C. A total of 5 beany odor-active compounds and 3 non-beany aroma-active compounds of this soymilk were analyzed by headspace solid phase microextraction (HS-SPME). As a result, lipoxygenase (LOX) activity gradually decreased by hot water treatment with time, and with the decrease of Lox activity, the 5 beany odor-active compounds and 3 non-beany aroma-active compounds were significantly decreased. However, the reduction in non-beany flavor compounds was smaller than for beany odor compounds. Therefore, a balance between beany and non-beany flavors can be reached in soymilk. When the soaked soybeans were blanched and ground with hot water for 2 to 6 min, the LOX activity was between 38% and 57% of the beginning activity. For these processing times, the non-beany compounds could be largely maintained. The ratio of the total peak area of the 3 non-beany aroma compounds and 5 beany flavor compounds was between 0.07 and 0.12, and the sensory scores of the aromas were higher than that of the off-flavors. Practical Application: Beany flavors in soymilk could be reduced with hot water blanching and grinding at temperature above 80 °C. However, the treatment of hot water blanching affected the non-beany aromas of soymilk. A suitable blanching and grinding time is necessary to achieve a balance of soymilk flavors.

Selected Odor Compounds in Soymilk As Affected by Chemical Composition and Lipoxygenases in Five Soybean Materials

Protein, fat content, and beany odor affect consumers' choice of soy foods. Our objective was to characterize protein, lipid, and lipoxygenase activities and fatty acid compositions in food soybeans and to determine how chemical composition and enzyme activities related to the generation of beany odor components, including hexanal, hexanol, trans-2-nonenal, 1-octen-3-ol, and trans-2,trans-4-decadienal in soymilk. Protein, lipid, and fatty acid compositions and lipoxygenase activities of five soybean materials, including Proto, IA2032, IA 2064, IA3017, and L-Star varieties, were analyzed. Soymilk was prepared by a traditional method involving soaking, grinding, and cooking processes. Selected odor compounds in raw and cooked soymilk were analyzed by solid-phase microextraction and gas chromatography. Results showed that soybeans differed in crude protein and lipid content, lipoxygenase activities, and fatty acid compositions. L-Star had the highest and Proto the lowest lipid content among the five soybean cultivars. Protein content, lipoxygenases, and linoleic acid were positively correlated with beany odor content in soymilk made from the selected soybean materials. After boiling for 20 min, the soymilk made from L-Star and IA2032 retained the lowest odor profiles among the soymilk products made from the five selected materials.

SENSORY CHARACTERISTICS OF COMBINATIONS OF CHEMICALS POTENTIALLY ASSOCIATED WITH BEANY AROMA IN FOODS

ABSTRACT Combinations of chemicals often associated with beaniness in foods were studied using descriptive sensory analysis. A highly trained descriptive panel evaluated combinations (two chemicals at a time) of chemicals containing either one beany and one non‐beany chemical, two beany chemicals, or two non‐beany chemicals to determine whether an overall beany characteristic was present. Overall beany character was found in various combinations from each grouping, including combinations of chemicals that alone were not considered beany. As in previous research, not all levels of chemicals imparted beaniness. When beaniness was present, the intensity was in the low range. The highest overall beany intensity was detected in the combination of 1‐octen‐3‐one, a beany chemical, and hexanal, a non‐beany chemical. Hexanal is abundant in volatiles of soybean products, but does not impart beany aroma individually. Also, combinations of hexanal and other non‐beany chemicals produced an overall beany odor that was not found in the individual chemicals.