Bean Varieties Research Articles

Exploring intra-allelic and inter-allelic gene interactions influencing seed yield and its components in inter-varietal crosses of Mungbean (Vigna radiata (L.) Wilczek)

Mungbean (Vigna radiata (L.) Wilczek) is a versatile legume widely cultivated for its nutritional value and adaptability. Meeting the increasing global demand for nutritious food requires the development of high-yielding varieties. Therefore, understanding the inheritance of yield and component traits is crucial for defining effective breeding strategies. In the present study, we aimed to investigate the genetic effects and interactions governing inheritance through generation mean analysis. The four crosses viz., IPM409-4×VGG18-002, IPM409-4×WGG42, COGG13-39×VGG16-058 and COGG13-39×VGG18-002 and their five generations (P1, P2, F1, F2, and F3) were evaluated for nine yield and yield component traits during 2023 summer season. The significance of additive, dominance, and epistatic components viz.,additive×additive [i] and dominance×dominance [l] of each trait was found to be different among all the crosses. Mungbean is a self-pollinated crop, so only fixable gene effects can be exploited for trait improvement. In the IPM409-4×VGG18-002 cross, all the traits exhibited additive or additive × additive gene action except for plant height and seed yield per plant (dominance). The scaling test was significant in IPM409-4×WGG42 cross for all the traits, except for the number of pods per cluster. Except for the number of branches per plant in which the dominance effect was evident, additive or additive×additive gene effects were observed for the other traits. In COGG13-39×VGG16-058 and COGG13-39×VGG18-002 crosses, all the yield traits recorded fixable (additive and additive×additive) gene effects except for number of pods per plant in COGG13-39×VGG18-002. Considering the results of all four crosses, gene actions that exhibit consistency across crosses revealed that epistatic interaction (additive×additive) significantly influenced the expression of various mung bean traits. Therefore, the later generation selection of short-duration segregants with high yield, bold seeds, and resistance to yellow mosaic disease from the above populations can be carried out to develop commercially valuable mung bean varieties.

Common bean polyphenolic enriched extracts decrease reactive oxygen species induced by heavy metals and polycyclic aromatic hydrocarbons in Hs27 and Hs68 human fibroblasts

The increase of coarse particulate matter (PM10) due to industrialization and urban sprawl has been identified as a significant contributor to air pollution and a threat to human skin health and premature aging. The objective was to analyze the antioxidant effect of phenolic-enriched extracts (PHE) obtained from black bean (BB) and pinto bean (PB) varieties (Phaseolus vulgaris L.) and pure phenolic compounds (rutin, catechin, and gallic acid) in two human dermal fibroblasts cell lines exposed to PM10. Petunidin-3-O-glucoside was the most abundant anthocyanin, with 57 ± 0.9 mg/g dry extract (DE) in PHE-BB. Gallic acid was the prevalent phenolic acid with 8.2 ± 2.8 mg/g DE in PHE-BB (p < 0.05). Hs27 and Hs68 cell lines were exposed to PM10 (100 μg/mL) to induce oxidative stress; PHE-BB reduced it by 69% ± 12 and PHE-PB by 80% ± 5 relative to PM10 treatment (p < 0.05). Delphinidin-3-O-glucoside showed the highest binding affinity in adenosine monophosphate-activated protein kinase (AMPK) with −9.0 kcal/mol and quercetin-3-D-galactoside with −6.9 kcal/mol in sirtuin 1 (Sirt1). Rutin increased the expression of Sirt1 by 30% (p < 0.05) in the Hs27 cell line treated with PM10. Common bean extracts can potentially reduce oxidative stress induced by PM10 in human dermal fibroblasts.

Mapping of flumioxazin tolerance in a snap bean diversity panel leads to the discovery of a master genomic region controlling multiple stress resistance genes.

Effective weed management tools are crucial for maintaining the profitable production of snap bean (Phaseolus vulgaris L.). Preemergence herbicides help the crop to gain a size advantage over the weeds, but the few preemergence herbicides registered in snap bean have poor waterhemp (Amaranthus tuberculatus) control, a major pest in snap bean production. Waterhemp and other difficult-to-control weeds can be managed by flumioxazin, an herbicide that inhibits protoporphyrinogen oxidase (PPO). However, there is limited knowledge about crop tolerance to this herbicide. We aimed to quantify the degree of snap bean tolerance to flumioxazin and explore the underlying mechanisms. We investigated the genetic basis of herbicide tolerance using genome-wide association mapping approach utilizing field-collected data from a snap bean diversity panel, combined with gene expression data of cultivars with contrasting response. The response to a preemergence application of flumioxazin was measured by assessing plant population density and shoot biomass variables. Snap bean tolerance to flumioxazin is associated with a single genomic location in chromosome 02. Tolerance is influenced by several factors, including those that are indirectly affected by seed size/weight and those that directly impact the herbicide's metabolism and protect the cell from reactive oxygen species-induced damage. Transcriptional profiling and co-expression network analysis identified biological pathways likely involved in flumioxazin tolerance, including oxidoreductase processes and programmed cell death. Transcriptional regulation of genes involved in those processes is possibly orchestrated by a transcription factor located in the region identified in the GWAS analysis. Several entries belonging to the Romano class, including Bush Romano 350, Roma II, and Romano Purpiat presented high levels of tolerance in this study. The alleles identified in the diversity panel that condition snap bean tolerance to flumioxazin shed light on a novel mechanism of herbicide tolerance and can be used in crop improvement.

Evaluation and Participatory Variety Selection of Common Bean (Phaseolus vulgaris L.) Varieties in Dawro Zone, South Western Region of Ethiopia

Farmers in the study area exhibit a unique tendency to choose varieties based on selection criteria rather than tested yields in the district. To ensure farmer participation, a study was conducted on six farmers’ farms, utilizing farmers as replicas in a Randomized Complete Block Design. Ten common bean crop varieties underwent evaluation on these farms to assess varieties in terms of farmer participation and identify priority criteria during the 2019 and 2020 production years. Plot sizes were standardized to 4m plot lengths with inter and intra-spacing of 40cm and 10cm, respectively. The seed and NPSB fertilizer rate were set at 100kg/ha and 122kg/ha, respectively. Significant differences were (P&lt;0.05) were observed among treatments for plant height, days to 50% flowering, days to 90% maturity, and yield over two years. Notably, the common bean varieties Remeda, DAB 96, and SER 119 yielded 1641, 1601, and 1588.7kg/ha, respectively. Additionally, the farmers in the district preferred red-seeded varieties for production and consumption due to superior seed per pod, pod per plant, seed size, seed color, and seed yield. Moreover, these farmers preferred varieties established tolerance to major common bean diseases which were common in the district. Consequently, these high-yielding, farmers preferred, and disease-tolerant varieties are recommended for demonstration and large-scale production in the study regions and agro-ecological zones similar to those the trial was implemented in south western region of Ethiopia.

Molecular, physiological and morphological markers assisted selection for salt-tolerant in some Egyptian faba bean varieties

Molecular, physiological and morphological markers assisted selection for salt-tolerant in some Egyptian faba bean varieties

Study of the nutritional quality of the seeds of two varieties of beans (Phaseolus vulgaris L.) grown in Congo

Phaseolus vulgaris L. is a legume belonging to the order Fabales and the family Fabaceae and which is consumed throughout the world for its seeds and pods. The seeds of this species provide a source of dietary protein available in many parts of the world; this is why they are considered “poor man’s meat” because of their high protein content and low cost, compared to animal proteins. The present study relating to the study of the nutritional value of bean seeds cultivated in Bouenza (Boko-Songho) and in the Plateaux (in Lékana) is a contribution to responding to the need for characterization of these varieties. Thus the general objective of this study which is to establish the nutritional value of these two local varieties “Kikata blanc” and “Lekana” is to use the standards established by the AOAC to achieve this objective, namely: the oven to determine the water content, the Soxhlet method to extract the fat, the Kjedahl method to determine the total protein content, the muffle furnace to know the ash rate, we deduced the carbohydrate content and finally we used spectrometry to identify the minerals in the ashes. We obtained the following results: Contents: Water: 7.65% ±0.07; Lipids: 0.81% ±0.02; Proteins: 10.6% ±0.14; Carbohydrates: 76.68% ±0.06; Ashes: 76.68% ±0.06; Energy value: 356.41 Kcal/100g. Among the minerals sought, we obtained: Phosphorus: 0.50% ±0.007; Magnesium: 0.14% ±0.007; Calcium: 0.04% ±0.006; Iron: 0.01% ±0.00. The results of this study allowed us to see that these two plants have good nutritional values. In order to complete this study, it is appropriate to look for other minerals in the ashes from these seeds; to deepen the studies to know the factors responsible for the differences in the biochemical composition observed between the two varieties.

Adaptation study of Haricot bean (Phaseolus vulgaris L.) varieties in moisture stress areas of Abergelle, Northern Ethiopia

Adaptation study of Haricot bean (Phaseolus vulgaris L.) varieties in moisture stress areas of Abergelle, Northern Ethiopia

Tuber Development and Tuber Yield Potential of Winged Bean (Psophocarpus tetragonolobus (L.) DC.), an Alternative Crop for Animal Feed

The winged bean is only one of the members of the leguminous species that has the potential for duo-purpose for its pod and tuber yields. The protein and nutritional content in the tuber can be used as alternative crops for animal feed. Previous studies identified the winged bean varieties as having high potential for tuber production, although their tuber development remains unclear. This study aims to elucidate the development of winged bean tubers based on their size and weight during eight months of growth. Tuber yields of high-potential winged bean varieties (W018 and W099) were compared to commercial varieties (Ratchaburi) under field conditions over two years. The experiments were laid out in randomized complete block design with three replications in the 2021–2022 and 2022–2023 growing seasons. The tuber formation of winged bean exhibited a significant increase in tuber weight and size in the fourth month of growth in all varieties, with a gradual change from fibrous roots to tubers. Particularly in the W018 and W099 varieties, tuber development persisted up to eight months after transplanting. On the other hand, the Ratchaburi variety had small tuber size, resulting in the tubers weighing less than in the W018 and W099 varieties. The results indicated that winged bean varieties had different tuber development, with the W018 and W099 varieties being suitable for tuber harvesting at eight months. The large tuber size can be an appropriate alternative to animal feed materials. At the same time, the Ratchaburi variety requires only five months since it is cultivated primarily for tuber consumption, which is concerned with size and shape. Moreover, the yields of the W018 and W099 varieties were significantly higher compared to Ratchaburi at 22.4, 19.3, and 7.423 T/ha in the 2021–2022 growing season and 8.4, 7.9, and 5.6 T/ha in the growing season of 2022–2023, respectively. The protein content in the winged bean tubers increased to 13.00% at eight months. The years (Y) and genotypes (G) differed considerably for tuber formation. The variation in both Y and G and the shoot/root ratio are also discussed in this study.

Are the sensory qualities of prestigious traditional varieties of beans preserved in commercial canned products? A comprehensive sensorial and chemical study on Ganxet beans

The commercial production of legume cans often involves exposing products to elevated temperatures, albeit with potential alterations to sensory traits. For premium products such as the Ganxet bean, which holds a Protected Designation of Origin (PDO) quality label, these adverse effects may influence consumer acceptance. This study investigated sensory and chemical differences between commercial cans and traditionally cooked Ganxet beans. A market survey enabled the identification of 15 commercial Ganxet cans, which were profiled for different physicochemical traits. Five were selected to represent maximum variability and were compared to two traditionally cooked references using standardized descriptive sensory and compositional analyses (volatile and non-volatile fractions). Commercial cans presented significantly higher off-flavor intensity and product darkening, while no significant changes were observed for textural traits. Commercial cans exhibited a broad diversity in their volatile profile and significantly lower content of distinctive non-volatile molecules (citric acid, sucrose, raffinose, and stachyose). A multivariate PLS regression model related off-flavor present in commercial cans with (E,E)-2,4-heptadienal, (E)-2-octenal, 1-nonanol (enhancers), and glutamic, malic, (E,E)-2,4-nonadienal, stachyose, sucrose, and glucose (inhibitors). The deviation of commercial Ganxet cans from PDO sensory quality requirements highlights the necessity for innovations in cooking protocols. These innovations are essential for producing high-quality cans from prestigious bean varieties.

Eliciting the aboveground physiological regulation that underlies salinity tolerance in faba bean (Vicia faba L.)

Salinity is a prevalent soil issue that reduces crop growth by a combination of osmotic stress and ion-specific toxicity. The crop response to salt stress is generally described in terms of a two phase model in which growth is initially reduced by osmotic stress and then Na+ toxicity. However, the relative importance of these mechanisms to salt stress is still not well understood. In this study a high-resolution image capture and analysis system was used to monitor shoot growth of faba bean plants non-destructively, while gas exchange measurements were used to examine the effects on photosynthesis in order to gain an understanding of the aforementioned stress mechanisms. The results of this soil-based study suggested that responses of crop growth to salinity stress depend on its severity: osmotic stress was the predominant cause of reduced growth at high levels of salinity, while specific-ion toxicity was more important under mild stress. We showed that the tolerant faba bean variety used dual mechanisms of ion exclusion and osmotic tolerance compared with the sensitive variety. Analysis of photosynthetic responses indicated that the extent to which stomatal closure affects photosynthetic capacity is indicated by the magnitude of the reduction in intercellular CO2 concentration.

Techno-functional properties and allergenicity of mung bean (Vigna radiata) protein isolates from Imara and KPS2 varieties

Mung bean is an increasingly cultivated legume. This study compared mung bean varieties ‘KPS2’ from Thailand (Th) and ‘Imara’ from Tanzania (T) with a focus on protein composition, allergenicity, and techno-functional properties. Two rounds alkaline-acid extraction were performed to produce mung bean protein isolate (MBPI - Th1/T1 and Th2/T2), supernatant (S) and protein-poor residue (PPR). Mass spectrometric analysis revealed high abundance of 8 s-vicilin and 11 s-legumin in MBPI and S. Extraction removed considerable amounts of the seed albumin allergen but increased the relative abundance of cupins in MBPI. Higher vicilin levels were found in Th1 samples, contributed to increased protein solubility above pH 6.5. Th formed stronger gels which were more stable at higher frequencies. In contrast, T proteins were structurally more flexible, leading to its improved foaming ability. This study provides the knowledge and methods for appropriate selection of mung bean varieties for various food applications.

Diversity and Phylogeny of Symbiotic Bacteria Nodulating Common Bean (Phaseolus vulgaris L.) in Côte d’Ivoire

Phaseolus vulgaris L. (common bean) is an agriculturally important legume that benefits from a symbiosis with bacteria belonging to the genus Rhizobium. Growing interest in the use of rhizobia as biofertilizers has led to the identification of a large number of rhizobia strains and studies of their diversity. Although much research has been carried out on rhizobia, there is little data on the diversity of rhizobia associated with common bean in Côte d'Ivoire. This study assessed the species diversity of common bean nodulating bacteria in ivorian soils. This diversity was assessed based on 16S rRNA gene sequencing. Ten high-performance bacterial isolates extracted from common bean nodules were used for genetic analysis. The 16S rRNA gene sequences of the native isolates were closely affiliated with members of the genera Rhizobium, Bradyrhizobium, Allorhizobium and Sinorhizobium demonstrating the presence of a diversity of native bean nodule bacteria. This study also reports for the first time the presence of Allorhizobium taibaishanense in common bean nodules. These results constitute an important step in the development of an effective microbial inoculum and sustainable food production.

Model‐based multi‐genotype characterization of drought stress Target Population of Environments for the common bean in East Africa

AbstractEfforts toward the development of common bean varieties that can perform better under the drought conditions in Eastern Africa are constrained by significant genotype–environment–management (G × E × M) interactions. To address this, an attempt was made previously to assess drought stress for Eastern Africa using the Target Population of Environments (TPE) approach, albeit by using a single cultivar. Here, we extend earlier TPE work with a larger sample of representative genotypes from East Africa including commercial and breeding lines. By using a larger genotype pool, we are able to (1) assess the extent to which TPEs identified vary across genotypes (i.e., generality of the TPEs) and (2) identify best‐performing genotypes for the various environments identified. To perform the analyses, we used the CROPGRO‐DRYBEAN cropping system model available in the Decision Support System for Agrotechnology Transfer (DSSAT) v4.7 calibrated and evaluated from field trials in Africa and Colombia with 10 genotypes. Our findings indicate that despite some level of genotype‐related variation, the dominant drought stress patterns, and their priorities—drought stress‐free conditions remain the dominant TPE, followed by terminal drought stress—remain unaltered. Moreover, we find that it is possible to reduce the extent and intensity of drought stress in various TPEs by adequately targeting genotypes from the pool considered herein. Importantly, the genotype characteristics for stress‐free, extreme terminal drought and moderate terminal drought stress are significantly different, confirming a differentiated breeding strategy is required between the TPEs. The findings from this study are useful for the modernization of the bean breeding programs in Africa.

First report of leaf spot caused by Colletotrichum camelliae in mung beans (Vigna radiata (L.) R. Wilczek) in South Korea.

Mung bean (Vigna radiata (L.) R. Wilczek) is a legume with high nutritional and economic value that is cultivated widely across Asia (Kang et al. 2014). In March 2022, a leaf spot disease in mung bean was observed at the Gangneung-Wonju National University Experimental farm (Gangneung, South Korea, 37.77°N, 128.86°E). The affected plants had irregular brown-gray leaf spots, and the bottom of the leaves showed concentric brown-gray rings that eventually progressed to necrotic lesions. Regardless of the cultivar, approximately 30% of the plants in the field were infected. To isolate the pathogen, the affected leaves were surface-sterilized by washing with 70% ethanol for 1 min, followed by washing with 2% NaClO for 2 min, then rinsing with sterile distilled water. We placed 3-mm sized diseased lesions on potato-dextrose agar (PDA), then incubated them at 27 ± 1 °C in the dark for 7 days and we obtained 1 isolate (CC1). The fungus on PDA had white aerial mycelia that became gray toward the center. Single spore cultures were obtained from fungal isolate. Isolated conidia were single celled, hyaline, cylindrical, and measured between 20.75 to 22.07 μm × 5.85 to 6.92 μm (n = 20), similar to other reports of C. camelliae(Wang et al. 2016). Mycelium from the single spore isolate was used for DNA extraction using Exgene™ Plant SV / (GeneAll®, Cat.No. 117-152), and we amplified the ITS region with primers ITS1 + ITS2 and ITS3 + ITS4, a portion of the actin gene with primers ACT-512F + 738R, and a portion of the beta-tubulin gene with primers BT2aF + BT2bR (Carbone et al. 1999; Glass et al. 1995; White et al. 1990). The amplified regions were sequenced by by Macrogen® (Seoul, South Korea). Sequences were deposited under GenBank accession numbers OR523262 (ITS), OR582483 (Actin), and OR566953 (beta-tubulin). MegaBLAST analysis of the ITS1, ITS2, ACT, and TUB regions showed 99% (216/217 bp) similarity with C. camelliae strain HNCS-26 (MK041440.1), 99% (303/305 bp) similarity with C. camelliae strain G3 (ON025203.1), 99% (242/244 bp) similarity with C. camelliae strain FWT41 (MN525820.1), and 99% (456/460 bp) with C. camelliae strain LF152 (KJ955239.1), respectively. To fulfill Koch's postulates, we conducted a pathogenicity teston the mung bean cultivar VC1973A (Seonhwanokdu) grown for four weeks at 25 °C with a 16-h day/8-h night cycle, simulating the field conditions when the symptoms were observed. We tested the pathogenicity on six plants , three control and three infected plants. Using three leaf replicates per plant resulting in total of nine leaves per group. Leaves were first injured using a sterile needle then either sterile 5 mm PDA plugs or plugs with C. camelliae were placed on the leaf for control and infected conditions, respectively. Irregular gray leaf spots were observed on the abaxial and adaxial of the infected leaf after 2 weeks, like the symptoms observed in the field. This was observed only on infected leaves and nowhere else on the plant and the control plants had no infection. We re-isolated the pathogen from diseased leaves and identified it as C. camelliae using the same molecular markers described previously, completing Koch's postulate. To the best of our knowledge, this is the first report of leaf spot caused by C. camelliae in mung bean plants in Korea, previously the fungi was reported to infect tea plants in Korea (Hassan et al. 2023). More studies are required to investigate potentially resistant mung bean varieties to minimize future damage caused by this fungus.

Response of Mixed Nano Micronutrients on Growth and Quality of French Bean

Micronutrients are essential for the enzymatic activity of plants, which affects fruit quality and growth. The study was conducted in the Rabi season of 2021 at SHUATS, Prayagraj. The purpose of the study was to investigate how the French bean variety "Arka Komal" was affected by mixed nano micronutrients in terms of growth and quality. The experiment was set up using a FRBD one factor being application of mixed nano micronutrients and other being application of nano fertilizers. The mixed nano micronutrients given were M0-control; M1- 0.2ml/ L of nano mix micronutrient/L of water as foliar application; M2- 0.4ml/L of nano mix micronutrient/ L of water as foliar application; M3 -0.6ml/ L of nano mix micronutrient/L of water as foliar application. The nano-fertilizers treatment given were F0– Control (without fertilizer); F1- 100% RDF as traditional fertilizer; F2-5ml/ L each of Nano NPK/ L of water as foliar application; F3- 4ml/L each of Nano NPK/ L of water as foliar application; F4- 3ml/L each of Nano NPK/ L of water as foliar application. 50% traditional fertilizers were applied in all treatments excluding control. From above experimental findings it was concluded that treatment M3 performed best for growth parameters like plant height; leaf area studied in French bean. M3 performed best for quality parameters like TSS, ascorbic acid content.

Differential Carbon Catabolite Repression and Hemicellulolytic Ability among Pathotypes of Colletotrichum lindemuthianum against Natural Plant Substrates.

Colletotrichum lindemuthianum is a phytopathogenic fungus that causes anthracnose in common beans (Phaseolus vulgaris) and presents a great diversity of pathotypes with different levels of virulence against bean varieties worldwide. The purpose of this study was to establish whether pathotypic diversity is associated with differences in the mycelial growth and secretion of plant-cell-wall-degrading enzymes (PCWDEs). We evaluated growth, hemicellulase and cellulase activity, and PCWDE secretion in four pathotypes of C. lindemuthianum in cultures with glucose, bean hypocotyls and green beans of P. vulgaris, and water hyacinth (Eichhornia crassipes). The results showed differences in the mycelial growth, hemicellulolytic activity, and PCWDE secretion among the pathotypes. Glucose was not the preferred carbon source for the best mycelial growth in all pathotypes, each of which showed a unique PCWDE secretion profile, indicating different levels of carbon catabolite regulation (CCR). The pathotypes showed a high differential hemicellulolytic capacity to degrade host and water hyacinth tissues, suggesting CCR by pentoses and that there are differences in the absorption and metabolism of different monosaccharides and/or disaccharides. We propose that different levels of CCR could optimize growth in different host tissues and could allow for consortium behavior in interactions with bean crops.

Improving the Growth and Productivity of Two Varieties of Mung Bean (Vigna radiata L.) by using Different Cultivation Methods and Dates

Background: Mung bean is characterized by its ability to withstand water scarcity due to its short life span, so it can be introduced into agricultural rotation systems and despite especially when there is a lack of humidity and contemporary environmental changes. Despite the importance of this crop, its productivity rate in Iraq is still low compared to global production. The dates and methods of cultivation of the mungbean crop must be determined accurately to suit the specifications of local varieties adapted to the changing environmental conditions. Methods: The field study was applied in two fields: the first in Nineveh Governorate and the second in Erbil Governorate / Republic of Iraq. Three factors included, first: two varieties of local mung bean (black and green), second: different cultivation methods (furrow and lines) and the third: planting dates (May 15, June 15 and July 15). The study was designed as a factor experiment a design (R.C.B.D) with three replicates. Result: The first variety superior significantly in the two sites with the qualities of (plant height, leaf area, leaf area index, seed yield, Biological yield and harvest index. From the results of the dates, we note a significant superiority of the first date number of seeds.pod-1 while the third date significantly outperformed the germination percentage for the two sites. First planting method significant superiority in most of the studied characterists and for the two research sites. Significant superiority was recorded for the interactions of research factors and their levels.

Synergistic effects of intercropping green beans with okra on the bacteria blight disease

Green bean is a widely consumed as vegetable mostly for its nutritional, health and economic values. In Cameroon, the production is hindered by bacterial blight disease cause by Xanthomonas campestris. The aim of this study is to evaluate the synergistic effects of intercropping green bean with okra on bacterial blight disease of green beans. Beans varieties (local green bean, jackpot green bean and dolly green bean) were planted in a field in a Randomized Complete Block Design with six treatments and three replicates in Department of Crop Production Technology of the University of Bamenda research farms. The first, second and third treatments consisted of inter-cropping of dolly green bean, jackpot green beans and local green with okra and fourth, fifth and sixth consisted of mono-cropped of dolly green bean, jackpot green beans and local green beans varieties which serves as the control experiments. Data was collected for disease incidence, disease severity, and number of pods and weight of pods were recorded in all the fields. Pathogenicity assessment was conducted in the green house by inoculating healthy green bean plants with bacterial blight isolate and lesion area measured. Significant variations (P≤0.05) were observed in disease incidence, disease severity and yield. The highest mean disease incident (100 %) severity (64 %)and low number of pods (4.3) were recorded in monocropped fields and the intercropped fields had lowest mean disease incidence(40 %), severity (23 %)and high number of pods (27.3 ).Inter-cropping green beans with okra can be recommended to control bacterial blight disease of green beans varieties.

Changes in biochemical composition of Ethiopian Coffee arabica with growing region and traditional roasting.

Updating the biochemical composition of coffee beans across the years is necessary. This is important to understand the vulnerability of coffee toward climate adaptation longitudinally. Accordingly, in this study the influence of growing area and traditional roasting on the biochemical composition of five common Ethiopian Arabica coffee beans collected in the harvest year of 2021/22 were investigated. With an average of 11.34 g/100 g, the Hararge and Jimma coffee beans had the highest crude fat content (p < 0.05). The crude protein content of the five varieties was in the range of 13-15 g/100 g, with respective highest and lowest contents in the (Hararge and Nekemte) and (Sidama and Yirgachefe) coffee beans (p < 0.05). The total phenolic content (TPC) of the coffee beans was in the order of Jimma (46.52) > Nekemte (44.55) > Sidama (44.31) > Hararge (39.02) > Yirgachefe (34.25) mg GAE/100 g. The 50% inhibitory concentration (IC50) of ascorbic acid, coffee bean extract from Jimma and Hararge against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical was 19.86, 20.22 and 20.02 μg/mL, respectively. The respective highest and lowest caffeine concentration was obtained in the Yirgachefe (10.38) and Hararge (7.55 g/100 g) coffee beans (p < 0.05). The Jimma, Sidama, and Nekemte coffee varieties had the highest chlorogenic acid content of 45 g/100 g (p > 0.05); whereas the lowest content was in Hararge coffee (36.78 g/100 g). While the caffeine concentration did not show significant (p > 0.05) difference, with all the coffee beans the roasting has reduced significantly the TPC, trigonelline and mainly the chlorogenic acid (p < 0.05). These data can update the existing facts on biochemical diversity of coffee beans in the country which can be used for evidence based innovations of climate adaptation in predicting the quality of coffee. Further recommendation of optimizing the traditional coffee processing method is supported from this study.

Effects Of Storage Container and Storage Condition on Seed Health Quality of Common Bean Variety Ennepa in Southern Ghana

Effects Of Storage Container and Storage Condition on Seed Health Quality of Common Bean Variety Ennepa in Southern Ghana