Bean Varieties Research Articles

Response Growth and Yield of Three Broad Bean Cultivars (Vicia faba L.) to Spraying with Different Concentrations of Salicylic Acid Under Saline Soil Conditions

South of Babylon province, at 32 degrees 40 minutes north latitude and 44 degrees 39 minutes east longitude, was the site of a field experiment run by the College of Agriculture at Al-Qasim Green University during the winter of 2022-2023. Three broad bean varieties were tested for their reactions to salicylic acid sprays of varying doses in saline soil. The experiment was conducted with a split plot arrangement according to The Randomized Complete Block Design (RCBD) with three replicates. The experiment included two factors: The first important factor was the cultivation of three cultivars of broad beans (Turkish, Spanish, and Dutch) which were placed in the main plot. As for, the sub-plot was included three concentrations of salicylic acid: control treatment, the spraying treatment with 100 mg L-1, and the spraying treatment with 200 mg L-1. The results showed that the Turkish cultivar was significantly excelled in the studied trait of plant height (118.46 cm), the number of branches (branch-1), and the biological yield (4.81 tons. ha-1), while the Spanish cultivar was excelled in leaf area (2505.8 cm2) and the number of pods (21.74 pods. plant -1), seed yield (2.49 ton. ha-1), and harvest index (56.15%). Where spraying with salicylic acid was significantly excelled on 200 mg L-1 in the studied traits for plant height (97.08 cm), the number of branches (10.87 branch.plant-1), the leaf area (2363.7 cm2), the number of pods (23.01 pods plant-1), seed yield (2.40 ton.ha-1), and the harvest index (49.23%).

The challenge of breeding for reduced off-flavor in faba bean ingredients.

The growing interest in plant protein sources, such as pulses, is driven by the necessity for sustainable food production and climate change mitigation strategies. Faba bean (Vicia faba L.) is a promising protein crop for temperate climates, owing to its remarkable yield potential (up to 8 tonnes ha-1 in favourable growing conditions) and high protein content (~29% dry matter basis). Nevertheless, the adoption of faba bean protein in plant-based products that aim to resemble animal-derived counterparts is hindered by its distinctive taste and aroma, regarded as "off-flavors". In this review, we propose to introduce off-flavor as a trait in breeding programs by identifying molecules involved in sensory perception and defining key breeding targets. We discuss the role of lipid oxidation in producing volatile and non-volatile compounds responsible for the beany aroma and bitter taste, respectively. We further investigate the contribution of saponin, tannin, and other polyphenols to bitterness and astringency. To develop faba bean varieties with diminished off-flavors, we suggest targeting genes to reduce lipid oxidation, such as lipoxygenases (lox) and fatty acid desaturases (fad), and genes involved in phenylpropanoid and saponin biosynthesis, such as zero-tannin (zt), chalcone isomerase (chi), chalcone synthase (chs), β-amyrin (bas1). Additionally, we address potential challenges, including the need for high-throughput phenotyping and possible limitations that could arise during the genetic improvement process. The breeding approach can facilitate the use of faba bean protein in plant-based food such as meat and dairy analogues more extensively, fostering a transition toward more sustainable and climate-resilient diets.

Farmer personality and community-based extension effectiveness in Tanzania

Community-based extension relies on social relationships with farmers to increase the trust in and effectiveness of extension activities. Personality traits play an important role in social outcomes. We develop a conceptual model which shows that farmer personality can influence the likelihood of being aware of and incorporating information from community-based extension activities. We then examine this heterogeneity empirically leveraging a previously implemented randomized control trial and a dataset of Big Six personality traits (agency, agreeableness, openness to experience, neuroticism, extraversion, and conscientiousness) for bean farmers in the Mbeya Region of Tanzania. We find that more extraverted farmers residing in villages where community extension agents were randomly selected to distribute trial packs of improved bean seed along with a demonstration plot comparison (rather than a demonstration plot comparison alone) were more likely to adopt the improved bean varieties. These farmers were also more likely to discuss bean farming with a larger proportion of farmers in their villages, indicating that the benefits of the trial pack treatment may have been greater for more sociable farmers. Our findings suggest that community-based extension effectiveness may be increased by considering the role of personality in the quality and quantity of farmers’ interactions within their communities.

Integration of comparative transcriptomics and WGCNA characterizes the regulation of anthocyanin biosynthesis in mung bean (Vigna radiata L.).

Mung bean is a dual-use crop widely cultivated in Southeast Asia as a food and medicine resource. The development of new functional mung bean varieties demands identifying new genes regulating anthocyanidin synthesis and investigating their molecular mechanism. In this study, we used high-throughput sequencing technology to generate transcriptome sequence of leaves, petioles, and hypocotyls for investigating the anthocyanins accumulation in common mung bean variety as well as anthocyanidin rich mung bean variety, and to elucidate their molecular mechanisms. 29 kinds of anthocyanin compounds were identified. Most of the anthocyanin components contents were significantly higher in ZL23 compare with AL12. Transcriptome analysis suggested that a total of 93 structural genes encoding the anthocyanin biosynthetic pathway and 273 regulatory genes encoding the ternary complex of MYB-bHLH-WD40 were identified, of which 26 and 78 were differentially expressed in the two varieties. Weighted gene co-expression network analysis revealed that VrMYB3 and VrMYB90 might have enhanced mung bean anthocyanin content by inducing the expression of structural genes such as PAL, 4CL, F3'5'H, LDOX, and F3'H, which was consistent with qRT-PCR results. These findings are envisaged to provide a reference for studying the molecular mechanism of anthocyanin accumulation in mung beans.

Effect of Angular Leaf Spot Disease on Bean Inter-cropped with Maize in Foumbot and Bambili, Cameroon

The ability to increase beans output at local levels is often hindered by farmer’s ability to control angular leaf sport disease caused by Phaeoisariopsis griseola, in the field. Studies were carried out in Foumbot and Bambili, using a Randomized Complete Block Design (RCBD) with four treatments and four replications in order to assess the impact of intercropping on angular leaf spot disease. Two varieties of bean were used (white dwarf and red dwarf marked beans). Treatments one and two consisted of mono-cropping of white dwarf and red dwarf marked bean varieties while treatments three and four consisted of the same varieties of beans inter-cropped with maize respectively. The data on disease incidence, disease severity, number of pods and pods weight were recorded in mono-cropped and inter-cropped fields. Pathogenicity assessment was carried out in the screen house by inoculating healthy plants with fungal isolate (3×104 spores / ml), during which the lesion diameter was measured. Beans varieties inoculated with spores’ suspension of P. griseola in the screen house showed symptoms identical to that of infected plants in the fields. The results obtained for disease severity and yield revealed that there was a significant difference (Pd”0.05) on the different treatments and in the different fields. The best yields and least disease severities of beans were observed in fields inter- cropped with maize because wind and rainfall splashes were intercepted by the plant which is not a host to P.gliseola thus a reduction of disease in the inter-cropped fields’ plant which is not a host to P.gliseola thus a reduction of disease in the intercropped fields. This shows that intercropping beans with maize can be used as a best management option to control angular leaf spot disease for the two varieties of beans used in this study.

Assessment of the Nutritional Value of Stems and Leaves of Australian Adzuki Bean.

Adzuki bean has recently been proposed as a viable dual-purpose (grain-and-graze) crop for the Northern regions of Australia because of its successful use in semi-arid regions and its nitrogen fixation capacity to improve soil fertility and animal nutrition. However, there are very few studies on the phytochemical composition and nutritional value of the non-seed material. This study investigated the phenolic composition of the parts grown in the vegetative phase (leaves and stems) of nine Australian adzuki bean varieties for the first time. The total phenolic content (TPC) of the stem material (157-406 mg GAE/100 g) was 23-217% higher than that of commercial livestock feed, while the TPC of the leaf material (1158-1420 mg GAE/100 g) was 9-11 times higher. Using tandem liquid chromatography mass spectrometry (LC-MS), the major phenolic compounds identified were rutin, luteolin, salicylic acid, and quercetin-3-glucoside. The leaf and stem materials showed high levels of apparent in vitro dry matter digestibility, with no significant difference in total gas or methane production compared to lucerne hay. The results suggest that adzuki bean vegetative materials could be a high-value livestock fodder and support pursuing further in-depth studies into their nutritional value for livestock.

A new framework for predicting and understanding flowering time for crop breeding

Societal Impact StatementAs the growing season changes, the development of climate resilient crop varieties has emerged as a crucial adaptation in agricultural systems. Breeding new varieties for a changing climate requires enhanced capacity to predict the complex interactions between genotype and environment that determine flowering time. Hundreds of experiments with observations of flowering, the environment and plant genetics were used to build a model that can predict when a variety of common bean is going to flower. This model will help breeders to explore the phenological characteristics of their germplasm, speeding up selection for climate adaptation.Summary There is an urgent need to accelerate crop breeding for adaptation to a changing climate. As the growing season changes, crop improvement programmes must ensure that the phenological characteristics of the varieties they develop remain well suited to their target population of environments. Meeting this challenge will require a clear understanding of how existing germplasm behave across Genotype ∗ Environment (G ∗ E) to enhance the efficiency of selection. Recent work calls for the development of simple models that can accurately simulate genotypic variation in key traits across target population of environments. Accordingly, we develop a simple machine learning framework for modelling time to flowering across G ∗ E and apply this to common bean in an equatorial target population of environments. Within this framework, we test three machine learning models and find that the best performing models display high levels of accuracy across G ∗ E. We advance understanding of the environmental drivers of flowering time in equatorial conditions by showing that thermal time and accumulated evaporation are powerful predictors of flowering time across all three models.

Relationship between phytochemical composition of five snap bean varieties and its susceptibility to aphid and whitefly infestation.

Relationship between phytochemical composition of five snap bean varieties and its susceptibility to aphid and whitefly infestation.

Characterizing patterns of seasonal drought stress for use in common bean breeding in East Africa under present and future climates

Common bean (Phaseolus vulgaris L.) is the second most important source of dietary protein and the third most important source of calories in Africa, especially for the poor. In East Africa, drought is an important constraint to bean production. Therefore, breeding programs in East Africa have been trying to develop drought resistant varieties of common bean. To do this, breeders need information about seasonal drought stress patterns including their onset, intensity, and duration in the target area of the breeding program, so that they can mimic this pattern during field trials. Using the Decision Support for Agrotechnology Transfer (DSSAT) v4.7 model together with historical and future (Coupled Model Inter-comparison Project 6, CMIP6) climate data, this study categorized Ethiopia, Tanzania, and Uganda into different target population of environments (TPEs) based on historical and future seasonal drought stress patterns. We find that stress-free conditions generally dominate across the three countries under historical conditions (50–80% frequency). These conditions are projected to increase in frequency in Ethiopia by 2–10% but the converse is true for Tanzania (2–8% reduction) and Uganda (17–20% reduction) by 2050 depending on the Shared Socioeconomic Pathway (SSP). Accordingly, by 2050, terminal drought stresses of various intensities (moderate, severe, extreme) are prevalent in 34% of Uganda, around a quarter of Ethiopia, and 40% of the bean growing environments in Tanzania. The TPEs identified in each country serve as a basis for prioritizing breeding activities in national programs. However, to optimize resource use in international breeding programs to develop genotypes that are resilient to future projected stress patterns, we argue that common bean breeding programs should focus primarily on identifying genotypes with tolerance to severe terminal drought, with co-benefits in relation to adaptation to moderate and extreme terminal drought. Little to no emphasis on heat stress is warranted by 2050s.

Automating high-throughput screening for anthracnose resistance in common bean using allele specific PCR

BackgroundCommon beans (Phaseolus vulgaris L.) provide important protein and calories globally. Anthracnose (Colletotrichum lindemuthianum (Sacc. & Magnus) Briosi & Cavara, 1889) is a major disease in common bean and causes significant yield losses in bean production areas. Screening for markers linked to known disease resistance genes provides useful information for plant breeders to develop improved common bean varieties. The Kompetitive Allele Specific PCR (KASP) assay is an affordable genetic screening technique that can be used to accelerate breeding programs, but manual DNA extraction and KASP assay preparation are time-consuming. Several KASP markers have been developed for genes involved in resistance to bean anthracnose, which can reduce yield by up to 100%, but their usefulness is hindered by the labor required to screen a significant number of bean lines. Our research objective was to develop publicly available protocols for DNA extraction and KASP assaying using a liquid handling robot (LHR) which would facilitate high-throughput genetic screening with less active human time required. Anthracnose resistance markers were used to compare manual and automated results.ResultsThe 12 bean anthracnose differential cultivars were screened for four anthracnose KASP markers linked to the resistance genes Co-1, Co-3 and Co-42 both by hand and with the use of an LHR. A protocol was written for DNA extraction and KASP assay thermocycling to implement the LHR. The LHR protocol reduced the active human screening time of 24 samples from 3h44 to 1h23. KASP calls were consistent across replicates but not always accurate for their known linked resistance genes, suggesting more specific markers still need to be developed. Using an LHR, information from KASP assays can be accumulated with little active human time.ConclusionResults suggest that LHRs can be used to expedite time-consuming and tedious lab work such as DNA extraction or PCR plate filling. Notably, LHRs can be used to prepare KASP assays for large sample sizes, facilitating higher throughput use of genetic marker screening tools.

Pertumbuhan dan Produksi Beberapa Varietas Kacang Hijau (Vigna radiata L) dengan Pemberian Beberapa Dosis Pupuk NPK

Abstract - The use of several varieties of green beans supported by NPK fertilizer and relatively easy cultivation techniques means this plant has good prospects as a business opportunity in the agribusiness sector because demand is quite large yearly. This research aimed to evaluate the appropriate dose of NPK fertilizer for the growth of several Mung Bean varieties. This research used a Randomized Block Design to treat several green bean varieties, Vima 1, Vima 2 and Vima 3. At the same time, the NPK fertilizer doses were P0 (without fertilizer), P1 (100 kg/ha), P2 (150 kg/ha) and P3 (200 kg /Ha). The research showed that fertilizer application only affected plant height, number of pods per plant and seed production per ha. At the same time, several varieties affected plant height and seed production per ha. The interaction of fertilizer application and several varieties significantly affects seed production per ha. Based on research results, applying fertilizer at a dose of 200 kg/ha gave the highest results in the growth and production of green beans. Based on the results of this research, it is also known that using several green bean varieties shows that the V-3 variety provides the best results in growth, and the V-2 variety provides the best results in production.

Variety-strain interaction specificity of <i>Bacillus subtilis</i> with salt-stressed <i>Phaseolus vulgaris</i> L. plants

The effectiveness of crop inoculation using growth-stimulating bacteria can depend on the plant variety and environmental conditions. The reactions of three-week-old plants of the Ufimskaya and Zolotistaya green bean varieties to inoculation with strains 26D and 10-4 in normal conditions and under stress (1% NaCl 48 h) were analysed. Although the Zolotistaya variety formed less biomass and leaf area, it surpassed the Ufimskaya variety in terms of chlorophyll and root water content, as well as reacting to stress by reducing the content of photosynthetic pigments and root water content. Inoculation contributed to the preservation of these indicators at the level of non–stressed plants: the positive effect of strain 26D was expressed in the effect on the composition of pigments, while that of strain 10-4 was expressed in the hydration of roots. Under stress, the Ufinskaya variety improved the water status of the roots, on which inoculation had a weak or negative effect; however, the decrease in the level of synthetic pigments under stress was compensated by inoculation with both strains. According to the biomass of three-week-old plants, the Zolotistaya variety was shown to respond positively to inoculation with both strains both normally and under stress, while the Ufimskaya variety tended to react negatively to inoculation with strain 26D; under stress, the effect of inoculation with both strains was positive. Compared with the stress-induced control, the malondialdehyde content in the roots of inoculated plants of both varieties decreased in comparison with that of the uninoculated control both in normal conditions and under stress. The revealed differences in plant reactions to inoculation serve as a basis for further analysis of the effectiveness of variety-strain combinations of symbiotic partners.

Evaluation of common bean genotypes against root rot complex pathogens in West Hararghe, Eastern Ethiopia

Common bean root rot caused by different fungal pathogens is an important disease affecting common bean (Phaseolus vulgaris L.) production and productivity. In Ethiopia, this disease has become one of the most destructive biotic constraints to common bean production. Information on common bean root rot disease management is lacking for the Ethiopian common bean production system in general and West Hararghe in particular. Therefore, in the 2018 main cropping season, a field experiment was performed on 19 common bean varieties in two research fields, the ‘Bareda and Oda Baso’ farmers’ training centers. The experiment was designed to evaluate the level of resistance of a common bean genotype against root rot complex pathogens under natural conditions using a Randomized Complete Block Design (RCBD) design with three replications. The levels of resistance were evaluated based on seedling emergence, disease incidence, and severity in parallel with grain yield data and subjected to analysis of variance (ANOVA). Based on the laboratory results, four pathogenic fungi, namely, Fusarium oxysporum f. sp. Phaseoli (F. o. f. sp. Phaseoli), Rhizoctonia solani (R. soni), Sclerotium rolfsii (S. rolfsii), and Macrophomina phaseolina (M. phaseolina), a Nematode sp., and stem maggot insect were identified. The combined analysis of variance showed very highly significant variation at (p < 0.0001) across the location. There was also very highly significant variation (p < 0.0001) among treatments except for seedling emergence, incidence, and severity, which were highly significant (p < 0.001). From the experiment, seven common bean varieties (Kufanzik, Roba, Hirna, SeR-125, Cranscope, Tinike, Awash-1) that showed resistance characteristics and two susceptible varieties (Choire and Argane) were selected and recommended.

Investigating the interactions of endornaviruses with each other and with other viruses in common bean, Phaseolus vulgaris

BackgroundPlant viruses of the genus Alphaendornavirus are transmitted solely via seed and pollen and generally cause no apparent disease. It has been conjectured that certain plant endornaviruses may confer advantages on their hosts through improved performance (e.g., seed yield) or resilience to abiotic or biotic insult. We recently characterised nine common bean (Phaseolus vulgaris L.) varieties that harboured either Phaseolus vulgaris endornavirus (PvEV1) alone, or PvEV1 in combination with PvEV2 or PvEV1 in combination with PvEV2 and PvEV3. Here, we investigated the interactions of these endornaviruses with each other, and with three infectious pathogenic viruses: cucumber mosaic virus (CMV), bean common mosaic virus (BCMV), and bean common mosaic necrosis virus (BCMNV).ResultsIn lines harbouring PvEV1, PvEV1 and PvEV2, or PvEV1, PvEV2 plus PvEV3, the levels of PvEV1 and PvEV3 RNA were very similar between lines, although there were variations in PvEV2 RNA accumulation. In plants inoculated with infectious viruses, CMV, BCMV and BCMNV levels varied between lines, but this was most likely due to host genotype differences rather than to the presence or absence of endornaviruses. We tested the effects of endornaviruses on seed production and seedborne transmission of infectious pathogenic viruses but found no consistent relationship between the presence of endornaviruses and seed yield or protection from seedborne transmission of infectious pathogenic viruses.ConclusionsIt was concluded that endornaviruses do not interfere with each other’s accumulation. There appears to be no direct synergy or competition between infectious pathogenic viruses and endornaviruses, however, the effects of host genotype may obscure interactions between endornaviruses and infectious viruses. There is no consistent effect of endornaviruses on seed yield or susceptibility to seedborne transmission of other viruses.

Risk Assessment of Residual Pesticides of Phaseolus vulgaris L. Consumed in Port Harcourt, Rivers State, Nigeria

Pesticides are important and necessary in reducing the loss caused by insect infestation on grains. However, its toxicity and persistence in the environment is of health concern. Beans (Phaseolus vulgaris L.) preserved with insecticide are considered to be good for consumption if its content of insecticide is not higher than the maximum residue limits (MRLs). The aim of this research was to quantify the residue of pesticide in four beans samples and determine the health risk associated with consuming these foods by residents of Port Harcourt, Rivers State, Nigeria. Pesticides content of beans samples were analyzed using Gas Chromatography (GC). The results of the study showed the presence of 17 different pesticide residues in all samples of beans. 2,4-Dichlorophenoxyacetic acid (2,4-D) and 2,2-dichlorovinyl dimethyl phosphate (DDVP) were detected in all samples of beans and at levels above European Union’s (EU's) MRL except in iron beans samples. Glyphosate was detected in all the samples at concentration above EU’s MRL. However, pesticides in Raw iron beans such as carbofuran (0.43±0.03 mg/kg), Endosulphan (0.18±0.01 mg/kg), HCB (0.62±0.10 mg/kg) Profenos (0.55±0.04 mg/kg) and t-nonachlor (0.32±0.00 mg/kg) decreased significantly to 0.23±0.10 mg/kg, 0.09±0.01 mg/kg, 0.31±0.01 mg/kg, 0.38±0.01 mg/kg, and 0.22±0.00 mg/kg respectively in Parboiled iron beans. This indicates reduced Health Risk Index (HRI) to pesticides of parboiled beans samples. Similar results were also recorded in other raw and parboiled beans varieties studied. This calls for the attention of regulatory agencies in foods and food products to effectively monitor the use and application of pesticides on foodstuff and also encourage parboiling before cooking to eat.

Informal Commercial Seed Systems: Leave, Suppress or Support Them?

Smallholder farmers require seed systems that can meet diverse functions: move a range of planting material; spread specialty varieties (climate-resilient or nutrient-dense varieties); reach last-mile areas; and perform in high-stress contexts. Acknowledging that smallholders use both formal and informal systems, this article focuses on the latter and on a component largely unexamined to date: informal commercial seed systems (ICSSs). Four evidence-based cases show how ICCSs contribute to varied seed system functions. In Tanzania, traders have moved multiple modern bean varieties countrywide and within just a few years. In the remote Ugandan north, traders have commercialized the sale of sweetpotato vines (produced off-season) to those lacking their own critical marshlands. In Bolivia, traders routinely sell native and modern varieties of seed tubers to farmers, along with their commerce in ware potatoes. In central Mali, a cluster of villages produces and sells pearl millet seed that is specially adapted to extreme drought conditions. All four cases share key characteristics: they distinguish seed vs. grain, serve local, regional, and international customers, and, perhaps most importantly, are sustained without subsidy or project support. As ICSSs meet farmers’ demands for seed that is not supplied by other actors, a question remains as to whether ICSSs should be left alone, leveraged, or improved further. Recognizing possible legal and operational challenges, this article suggests that ICSSs first be studied in-depth—characterizing their variations, locales, and system functions—so that future debates on possible support can be grounded in concrete evidence of ICSSs’ strengths, weaknesses, and unique benefits.

Maximizing Productivity of some Faba Bean Varieties by Foliar of Wood Vinegar and Algae under Sandy Soil Conditions

Maximizing Productivity of some Faba Bean Varieties by Foliar of Wood Vinegar and Algae under Sandy Soil Conditions

Technical efficiency of early variety country bean cultivation in selected areas of Bangladesh: a stochastic frontier approach

The present study was undertaken to measure the profitability and technical efficiency of early variety country bean cultivation in selected areas of Chatmohar upazila of Pabna district. A total of 60 early variety country bean-growing farmers were selected from three villages of Chatmohar upazila using a simple random sampling technique. Stochastic frontier production function was applied to determine the technical efficiency of the country bean growers. Technical inefficiency effects model was also applied to identify the demographic and socio-economic factors by which inefficiency effects are influenced. The study revealed that Benefit Cost Ratio (BCR) was the highest (2.29) for large farmers followed by medium (2.27) and small farmers (2.21). The result of the Maximum Likelihood Estimates (MLE) showed that human labor, fencing and manure had significant positive impact whereas the irrigation and insecticides had a negative impact on the production of country bean. The results of technical inefficiency effect model showed that farmer’s age, farm size had negative impact while education, family size and farm credit had positive impact on technical efficiency. The inefficiency analysis results indicated that farm level technical efficiency ranged between 43.06% and 98.01% with a mean technical efficiency of 91.9% implied that there was potential to increase country bean production among smallholder farmers in the study area by 8.1% through efficient use of present technology. The main constraints to country bean (early variety) production were identified as- infestation of pest and disease, high price of inputs, paucity of quality seed and fertilizer, scarcity of human labor, lack of technical knowledge, limited access to institutional credit etc. Despite the constraints, there is a great prospect of early variety country bean production in the study areas. Country bean (early variety) production could be increased in a sustainable manner if farmers would be provided with government support regarding lower price of input, fair price of output, marketing facilities, training for farmers and farm credit at low interest rate, reducing the activities of syndicate of middlemen. Government should provide necessary support to the farmers for ensuring sustainable production of early varieties of country bean. Res. Agric. Livest. Fish. Vol. 10, No. 2, August 2023: 155-164

Fluoride stress affects seed germination and seedling growth by altering the morpho-physiology of an African local bean variety

Increased fluorine pollution represents a serious limitation for the productivity of important crops such as beans. The present study was conducted to detect antagonistic/synergistic ion mobility during seed germination in the presence of F contamination (KF and NaF). NaCl was used as a benchmark. The results showed that germination of Jesca, an African (Tanzania) bean variety, significantly dropped with high F levels (10% KF and 3% NaF). High F levels reduced Jesca growth and decreased root and shoot biomass (by 50% and 95% with KF and NaF, respectively). NaF 200 mg kg−1 had the most depressive effect on the seedling stage. Elevated F levels negatively affected seedling health, revealing toxicity symptoms such as chlorophyll degradation and low photosynthetic activities that degraded after a threshold level of 80 mg kg−1. In addition, an inhibitory effect of F on the mineral status of the seedlings, especially on the Ca content, was observed. An opposite trend of endogenous Ca response to NaCl stress was observed. Indeed, while endogenous Ca content increased with increasing NaCl concentration, it decreased when the F level increased. Therefore, tolerance to F at the germination and seedling stages might be used as a criterion for selecting F-tolerant bean varieties.

Yield Components of Red Yardlong Bean Pod Genotypes (Vigna unguiculata (L.) ssp. sesquipedalis)

One of the legume plants with development potential is the yardlong bean. Because they have a high anthocyanin content, yardlong bean red pods are crucial for maintaining human health. There are multiple red-purple pod lines as a result of the crossing of the two parents purple pod and green pod. The purpose of this study was to assess the yield component of bean lines that were thirteen yards long. The study was carried out in Tembalang, Semarang, Central Java’s Agrotechno Park. Using genotype as the only component, the experiment used a randomized complete block design (RCBD). Agronomic characteristics such as harvesting time, number of pods, pod weight, length, number of seeds per pod, and weight of 1000 seeds were observed. The result showed that one genotype from F7 generation FPT A 3-4-10-1 had superior characters compared to the cultivar check in harvesting time, the number of pods, pod weight, and the number of seeds per pod. The agronomic characters that has been characterized can be may be used as potential donors for future hybridization programs to develop superior yard long bean variety with good yield components.