AbstractHINOTORI (Hybrid Integration Project in Nobeyama, Triple-band Oriented) has constructed a higher sensitivity 22/43 GHz and a 22/43/86 GHz simultaneous observation systems in the Nobeyama 45-m telescope by introducing new frequency separation filters in the telescope’s quasi-optics. The performance of the observation systems, such as the beam squint, the aperture efficiency, the system noise temperature when inserting the filters, and the phase stability of the signal path have been evaluated. It is indicated that the established systems have sufficient performance for single-dish and VLBI observations. The single-dish observation demonstrations using the triple-band system were successfully conducted in acquiring scientific data including multiple maser lines.