This paper develops a twist beam for multi-directional energy harvesting using piezoelectric transduction. The working principle of the multi-directional ability is explained by the theory of a pre-twisted beam. A simulation study is performed for concept verification and revealing the potential advantages of the proposed twist-beam vibration energy harvester (VEH). A physical prototype is fabricated and an experimental study is conducted to evaluate the actual performance of the proposed twist-beam VEH for validation. The results show that for the excitation comes from any direction in the plane in parallel with the beam cross-section, the proposed twist-beam VEH can produce a substantial amount of power. The optimal resistance is independent of the excitation direction. Moreover, it is found that the relationships between the output voltage amplitude and the excitation direction are different for the cases around the first and second resonances of the twist-beam VEH. The underlying physics behind this phenomenon is that the dominant motion of the two resonant modes are contrarily different. In addition, the experimental study shows that the first two natural frequencies of the prototyped twist-beam VEH are 22.37 and 41.47 Hz, respectively, which are much closer to each other as compared with those of a conventional plain beam. This feature benefits multi-modal energy harvesting within a targeted frequency range. The prospects regarding further optimization of the proposed twist-beam VEH are discussed.