Vidy Bay Research Articles

Diversity, Functions and Antibiotic Resistance of Sediment Microbial Communities From Lake Geneva Are Driven by the Spatial Distribution of Anthropogenic Contamination.

Lake sediments are natural receptors for a wide range of anthropogenic contaminants including organic matter and toxicants such as trace metals, polycyclic aromatic hydrocarbons, polychlorinated biphenyls that accumulate over time. This contamination can impact benthic communities, including microorganisms which play a crucial role in biogeochemical cycling and food-webs. The present survey aimed at exploring whether anthropogenic contamination, at a large lake scale, can influence the diversity, structure and functions of microbial communities associated to surface sediment, as well as their genetic potential for resistance to metals and antibiotics. Changes in the characteristics of these communities were assessed in surface sediments collected in Lake Geneva from eight sampling sites in October 2017 and May 2018. These sampling sites were characterized by a large concentration range of metal and organic compound contamination. Variation between the two sampling periods were very limited for all sampling sites and measured microbial parameters. In contrast, spatial variations were observed, with two sites being distinct from each other, and from the other six sites. Benthic communities from the most contaminated sampling site (Vidy Bay, near the city of Lausanne) were characterized by the lowest bacterial and archaeal diversity, a distinct community composition, the highest abundance of antibiotic resistance genes and functional (respiration, denitrification, methanogenesis, phosphatase, and beta-glucosidase) activity levels. The second sampling site which is highly influenced by inputs from the Rhône River, exhibited low levels of diversity, a distinct community composition, high abundance of antibiotic resistance genes and the highest bacterial abundance. Overall, our results suggest that local anthropogenic contamination, including organic matter and toxicants, is a major driver of the diversity and functioning of sediment-microbial communities in Lake Geneva. This highlights the need to consider benthic microbial communities and a suite of complementary ecotoxicological endpoints for more effective environmental risk assessments of contaminants in lake sediments.

The Foreign Oligochaete Species Quistadrilus multisetosus (Smith, 1900) in Lake Geneva: Morphological and Molecular Characterization and Environmental Influences on Its Distribution.

Simple SummaryThe presence of the oligochaete species Quistadrilus multisetosus (Smith, 1900), originating from North America, has been mentioned in Europe for some decades and was recently found in Swiss lakes. Here, we report its repartition and abundance in Lake Geneva based on morphological and eDNA surveys and study its ecology and invasive potential in this lake. We also provide an identification key of this species and two closely related species and describe the phylogenetic position of Q. multisetosus within several Tubificinae lineages based on the cytochrome c oxidase marker. Our results showed that this species was restricted to an area close to the outlet of a wastewater treatment plant and to a combined sewer overflow, was highly tolerant to organic matter pollution and had a limited capacity to disseminate in this lake. Even if the trophic status (oligo-mesotrophic) of Lake Geneva seems unfavorable for the development of this species, we recommend continuing monitoring its presence in this lake in the future, as the current warming of waters could contribute to its expansion.The presence of the oligochaete species Quistadrilus multisetosus (Smith, 1900) originating from North America has been mentioned for several decades in Europe, the Middle East and Russia. Its distribution and abundance in Europe is still unknown but it can be considered as potentially invasive. This species was recently discovered in Lake Geneva (Switzerland/France) and three other Swiss lakes. The aims of the present work are to report its repartition and abundance in Lake Geneva, to study its ecology and to determine its invasive potential in this lake. We also provide an identification key for correctly differentiating Q. multisetosus from the closely related species Spirosperma ferox Eisen, 1879 and Embolocephalus velutinus (Grube, 1879), and study the phylogenetic position of Q. multisetosus within several Tubificinae lineages based on the cytochrome c oxidase (COI) marker. Twenty-eight sites have been monitored since 2009 in Lake Geneva. In several sites, the COI sequence corresponding to this species was also searched for in sediment samples using high-throughput sequencing. In addition, we examined specimens collected in this lake before 2009 likely to belong to Q. multisetosus and to have been misidentified. We found that Q. multisetosus was only present in the lake downstream of a wastewater treatment plant and a combined sewer overflow in the Vidy Bay (near Lausanne) and at a site located nearby. These results confirmed the high tolerance of this species to organic matter pollution. Q. multisetosus was already present in this location in 1974 (misidentified as Spirosperma ferox), which suggests that Q. multisetosus has a limited capacity to disseminate in this lake. However, we recommend continuing monitoring its presence in Lake Geneva in the future, especially in the context of warming of waters that could contribute to the expansion of this species.

Elevated Concentrations of 4-Bromobiphenyl and 1,3,5-Tribromobenzene Found in Deep Water of Lake Geneva Based on GC×GC-ENCI-TOFMS and GC×GC-μECD.

We quantified the concentrations of two little-studied brominated pollutants, 1,3,5-tribromobenzene (TBB) and 4-bromobiphenyl (4BBP), in the deep water column and sediments of Lake Geneva. We found aqueous concentrations of 625 ± 68 pg L–1 for TBB and 668 ± 86 pg L–1 for 4BBP over a depth range of 70–191.5 m (near-bottom depth), based on duplicate measurements taken at five depths during three separate 1 month sampling periods at our sampling site near Vidy Bay. These levels of TBB and 4BBP were 1 or 2 orders of magnitude higher than the quantified aqueous concentrations of the components of the pentabrominated biphenyl ether technical mixture, which is a flame retardant product that had a high production volume in Europe before 2001. We observed statistically significant vertical concentration trends for both TBB and 2,2′,4,4′,6-pentabromobiphenyl ether in the deep water column, which indicates that transport and/or degradation processes affect these compounds. These measurements were enabled by application of a comprehensive two-dimensional gas chromatograph coupled to an electron capture negative chemical ionization time-of-flight mass spectrometer (GC×GC-ENCI-TOFMS) and to a micro-electron capture detector (GC×GC-μECD). GC×GC-ENCI-TOFMS and GC×GC-μECD were found to be >10× more sensitive toward brominated pollutants than conventional GC×GC-EI-TOFMS (with an electron impact (EI) ionization source), the latter of which had insufficient sensitivity to detect these emerging brominated pollutants in the analyzed samples. GC×GC also enabled the estimation of several environmentally relevant partitioning properties of TBB and 4BBP, further confirming previous evidence that these pollutants are bioaccumulative and have long-range transport potential.

High-resolution reconstruction of the 20th century history of trace metals, major elements, and organic matter in sediments in a contaminated area of Lake Geneva, Switzerland

Toxic trace metals in lacustrine sediments are of major concern since they can be hazardous to biota and human health. A high-resolution multiproxy study, including trace metals and major elements (measured by ICP-MS and XRF), total organic carbon, mineral carbon, Hydrogen Index, Oxygen Index, and C/N ratios, was performed on a sediment core from Vidy Bay in Lake Geneva. This bay has been affected by hazardous compounds released via the sewage effluent of a major wastewater treatment plant (WWTP). Anthropogenic trace metals, such as Pb, Cd, Cu, Zn, and Hg, increased following the industrial revolution in Europe. The highest amounts of these toxic metals, together with Ni, Cr, Co, Ag, Bi, and Fe, were recorded in sediments from 1964, the date of the WWTP implementation. During this period, all trace elements exceeded the sediment quality guideline “probable effect concentration” (PEC), with the following maximum concentrations (in mg kg−1): Pb 4000, Cd 23, Cu 1200, Zn 8600, Hg 11, Ni 140, Cr 270, Ag 130, and Bi 310. The geochemistry of detrital elements (Al, Si, Ca, Ti, K, Zr, Rb, and Sr), as well as S, Fe, P, and the nature and quality of organic matter were clearly also affected by the effluent. The sedimentary record revealed that, after some improvements in the wastewater treatment processes and the relocation of the outlet pipe, the sediments tended to return to concentrations similar to those prevailing before the WWTP implementation. However, despite the reduction in the contamination load from the WWTP, which could be reinforced with the construction of a new plant in the near future, the sediments deposited in Vidy Bay represent a major contaminant legacy that constitutes a potential threat to the lake biota in the case of sediment remobilization.

The influence of bottom boundary layer hydrodynamics on sediment focusing in a contaminated bay.

Understanding the dynamics and fate of particle bound contaminants is important for mitigating potential environmental, economic and health impacts linked to their presence. Vidy Bay, Lake Geneva (Switzerland), is contaminated due to the outfall and overflow from the wastewater treatment plant of the City of Lausanne. This study was designed to investigate the fate of particle-bound contaminants with the goal of providing a more complete picture of contaminant pathways within the bay and their potential spread to the main basin. This goal was achieved by investigating the sediment transport dynamics, using sediment traps and radionuclide tracers, and ascertaining how local bottom-boundary hydrodynamic conditions (temperature, turbidity, current velocity and direction) influence these dynamics. Results of the study indicated that sedimentation rates and lateral advections increased vertically with proximity to the lakebed and laterally with proximity to shore, indicating the presence of sediment focusing in the bay. Hydrodynamic measurements showed the persistent influence of a gyre within the bay, extending down to the lake bed, while just outside of the bay circulation was influenced by the seasonal patterns of the main basin. Calculated mean displacement distances in the bay indicated that suspended particles can travel ∼3 km per month, which is 1.7 times the width of the Vidy Bay gyre. This results in a residence time of approximately 21 days for suspended particles, which is much greater than previously modelled results. The calculated mobility Shield parameter never exceeded the threshold shear stress needed for resuspension in deeper parts of the bay. In such, increased lateral advections to the bay are not likely due to local resuspension but rather external particle sources, such as main basin or shallow, littoral resuspensions. These external sources coupled with an increased residence time and decreased current velocity within the bay are the precipitating factors in sediment focusing. While the spread of contaminants from the bay may occur through the transport of fine suspended sediments in shallower zones of the bay (<60 m) by longshore littoral currents, results suggest that particle-bound contaminants are likely to remain within the bay.

Application of δ18O, δ13CDIC, and major ions to evaluate micropollutant sources in the Bay of Vidy, Lake Geneva

Waters were sampled monthly from a profile at the wastewater outlet and a reference point in the Bay of Vidy (Lake Geneva) for a year. The samples were analyzed for 18O/16O of water, 13C/12C of dissolved inorganic carbon (DIC), major ions, and selected micropollutant concentrations. δ18O values, combined with the major ion concentrations, allowed discharged waste and storm-drainage water to be traced within the water column. On the basis of δ18O values, mole fractions of wastewater (up to 45 %), storm-drainage (up to 16 %), and interflowing Rhône River water (up to 34 %) could be determined. The results suggest that the stormwater fractions do not influence micropollutant concentrations in a measurable way. In contrast, the Rhône River interflow coincides with elevated concentrations of Rhône River-derived micropollutants in some profiles. δ13C values of DIC suggest that an increase in micropollutant concentrations at the sediment–water interface could be related to remineralization processes or resuspension.

Into the abyss of Lake Geneva: the elemo interdisciplinary field investigation using the MIR submersibles

In summer 2011, the two Russian MIR submersibles were brought to Switzerland to perform deep water dives in Lake Geneva. Research teams from several environmental science institutes, both national and international, participated in this interdisciplinary effort to investigate the deeper parts of Lake Geneva. Using the MIRs allowed the scientists to see and precisely select the sites where they could extract specific sediment cores and carry out detailed in situ measurements at the sediment–water boundary. One focus site was the surrounding of the outlet of the wastewater treatment plant of the City of Lausanne, which discharges into the Vidy Bay. The investigations concentrated on the pollution of the local sediments, pollution-related ecotoxicological risks, microbial activity and spreading and removal of the effluents from the bay to the open waters of the lake. The other focus site was the Rhone River delta and its subaquatic canyons, which formed as a result of the long-term interplay of the deposition of river-borne sediments and flood-triggered canyon erosion events.

Colloid characterization at the sediment-water interface of Vidy Bay, Lake Geneva

Colloids play a critical role in the transport of particle-bound contaminants. Knowledge of colloids and their aggregates provides insight into contaminant transport and fate within a given aquatic environment. Here, colloids and aggregates at the sediment-water interface of Vidy Bay, Lake Geneva, Switzerland, were characterized with a combination of analytical techniques to understand their structure, size distribution, concentration, and stability (the potential for aggregation). Vidy Bay is known to be the most contaminated part of Lake Geneva, being influenced by the effluents of a municipal wastewater treatment plant. Colloids were a heterogeneous mix of inorganic constituents (diatom fragments, quartz, clay, endogenic calcite, iron oxy-hydroxides) bridged together by rigid biopolymer strands or gels. The presence of rigid biopolymers was quite significant and they were typically found to have iron oxy-hydroxides embedded within their structure. Ion chromatographic data were comparable to previous values attained for the water column of Lake Geneva; however, single particle counting results indicated the presence of a nepheloid layer in Vidy Bay. In such, the stability of colloids was likely influenced by their proximity to the sediment-water interface. Zeta potential results inferred charge neutralization and destabilization of colloids and aggregates. Self-assembly of rigid biopolymers, along with cationic bridging between rigid biopolymers and inorganic constituents readily aggregated colloids. Taken together, colloids at the sediment water interface of Vidy Bay appeared to be unstable and to play a minor role in the transport of contaminants over long distances.

Wastewater as a point source of antibiotic-resistance genes in the sediment of a freshwater lake

Antibiotic-resistance genes (ARGs) are currently discussed as emerging environmental contaminants. Hospital and municipal sewage are important sources of ARGs for the receiving freshwater bodies. We investigated the spatial distribution of different ARGs (sul1, sul2, tet(B), tet(M), tet(W) and qnrA) in freshwater lake sediments in the vicinity of a point source of treated wastewater. ARG contamination of Vidy Bay, Lake Geneva, Switzerland was quantified using real-time PCR and compared with total mercury (THg), a frequently particle-bound inorganic contaminant with known natural background levels. Two-dimensional mapping of the investigated contaminants in lake sediments with geostatistical tools revealed total and relative abundance of ARGs in close proximity of the sewage discharge point were up to 200-fold above levels measured at a remote reference site (center of the lake) and decreased exponentially with distance. Similar trends were observed in the spatial distribution of different ARGs, whereas distributions of ARGs and THg were only moderately correlated, indicating differences in the transport and fate of these pollutants or additional sources of ARG contamination. The spatial pattern of ARG contamination and supporting data suggest that deposition of particle-associated wastewater bacteria rather than co-selection by, for example, heavy metals was the main cause of sediment ARG contamination.

Influence of a wastewater treatment plant on mercury contamination and sediment characteristics in Vidy Bay (Lake Geneva, Switzerland)

Previous direct observations of the sediment surface in Vidy Bay, Lake Geneva (Switzerland), revealed a range of sediment characteristics in terms of colour, texture and morphology. Dives with the MIR submersibles during the éLEMO project permitted the exploration of a large portion of Vidy Bay. It is the most contaminated part of Lake Geneva, due to inputs of treated and untreated waters from a large wastewater treatment plant (WWTP). To evaluate the influence of WWTP effluent on mercury contamination and sediment characteristics, 14 sediment cores were retrieved in the vicinity of the wastewater treatment plant effluent. Total mercury concentrations in sediments ranged between 0.32 and 10.1 mg/kg. Inorganic mercury and monomethylmercury concentrations in overlying and pore waters were also measured. The total partition coefficients of mercury (logK d) ranged from 3.6 to 5.8. The monomethylmercury concentration in pore waters of surface sediments was a large proportion of the total mercury concentration (44 ± 25 %). A Spearman test showed a negative correlation between the distance to the wastewater treatment plant outlet and the concentrations of total mercury in sediments and pore waters. Visual observations from the submersible allowed recognizing six different types of sediment. The areal distribution of these different sediment types clearly showed the influence of the wastewater treatment plant outlet on the sediment surface patterns. However, no relationship with mercury concentrations could be established.

Trace metal speciation at the sediment–water interface of Vidy Bay: influence of contrasting sediment characteristics

Trace metal analysis and speciation were performed at the sediment–water interface of Vidy Bay (Lake Geneva, Switzerland). This bay is impacted by hazardous compounds released via the sewage effluent of a major wastewater treatment plant (WWTP). Sediment cores and overlying water were sampled simultaneously at 12 sites characterized by contrasting sediment surface characteristics (color, methanogenic activity, bacterial mat) using corers deployed from a MIR submarine or research boat. The concentrations of trace metals in particulate form in the sediment and dissolved in the interstitial water, as well as the particulate, colloidal and dynamic fractions of trace metals in the overlying water were determined by combining an in situ and laboratory multi-method analytical approach. The results indicate differences in trace metal speciation in the sediment and overlying water at the 12 investigated sites. The observed differences were found to be more correlated to bacterial community, abundance, type and activity than to distance from the WWTP sewage outlet.

Bacterial communities in trace metal contaminated lake sediments are dominated by endospore-forming bacteria

Lake sediments in areas close to the outlet of wastewater treatment plants are sinks for pollutants. Bacterial communities in sediments are likely affected by the released effluents, but in turn they might modify the distribution and bioavailability of pollutants. On the shore of Lake Geneva, Switzerland, wastewater from the City of Lausanne is treated and discharged into the lake via an outlet pipe in the Vidy Bay. The objectives of this study were to assess (1) the impact of the treated wastewater release on the bacterial communities in the Vidy Bay sediments and (2) the potential link between bacterial communities and trace metal sediment content. Bacterial community composition and abundance were assessed in sediments collected in three areas with different levels of contamination. The main factors affecting bacterial communities were inferred by linking biological data with chemical analyses on these sediments. Near to the outlet pipe, large quantities of bacterial cells were detected in the three upper most cm (3.2 × 109 cells assessed by microscopy and 1.7 × 1010 copies of the 16S rRNA gene assessed by quantitative PCR, per gram of wet sediment), and the dominant bacterial groups were those typically found in activated sludge (e.g. Acidovorax defluivii and Hydrogenophaga caeni). Three samples in an area further away from the outlet and one sample close to it were characterized by 50 % of endospore-forming Firmicutes (Clostridium spp.) and a clear enrichment in trace metal content. These results highlight the potential role of endospore-forming Firmicutes on transport and deposition of trace metals in sediments.

Direct effects of dominant winds on residence and travel times in the wide and open lacustrine embayment: Vidy Bay (Lake Geneva, Switzerland)

Numerical simulations were carried out to determine the residence (or flushing) time of water in Vidy Bay (north shore of Lake Geneva) for different meteorological conditions. A hydrodynamic model (Delft3D-FLOW) was applied to simulate the flow field in the embayment during 2010 and January 2011. Using these results, particle tracking was applied to estimate transport of wastewater effluent discharged into the embayment. The model predictions compared well with published field measurements of dissolved species (as given by electrical conductivity profiles) within the wastewater. The pelagic boundary of the embayment was defined by the largest within-bay gyre. Based on this definition, particle tracking was used to quantify the residence time under dominant wind conditions. Similarly, particle tracking was used to determine the travel time (i.e., time to exit the embayment) for each of Vidy Bay’s three inflows (stream, stormwater and wastewater effluent). Although the wind field over the lake is variable, current patterns in the embayment can be simulated using the hydrodynamic model forced by a spatially uniform wind field. For a given wind speed, the main factor influencing residence and travel times is the wind angle. The presence of gyres leads to high mean residence times with large variability. As the wind direction becomes more aligned with the shoreline (i.e., with increasing westerly or easterly components), longshore currents dominate. These disrupt gyre formation and markedly reduce the mean and variability of embayment residence time. The numerical model was utilized to assess the potential for plume movement (in plan) from above the wastewater effluent outfall towards one of Lausanne’s drinking water intakes. In the most direct pathway, westward longshore currents can move water from the embayment to the water column above the intake location.

Iron isotope geochemistry of biogenic magnetite-bearing sediments from the Bay of Vidy, Lake Geneva

Dissimilatory microbial iron oxide reduction (DIR) has been hypothesized to be an important respiratory pathway on early Earth, potentially generating significant quantities of Fe(II) that have been preserved in Proterozoic and Archean sedimentary rocks. In particular, DIR has been implicated in the formation of magnetite in Precambrian marine sediments. To date, however, only one modern sedimentary environment exists where in situ magnetite formation has been linked to DIR: the Bay of Vidy in Lake Geneva, Switzerland. Previous work at this locality has characterized a magnetic susceptibility anomaly that reflects the presence of fine-grained magnetite produced via microbial reduction of amorphous Fe(III) oxides that enter the Bay of Vidy from a nearby sewage treatment plant. In this study, we report on the Fe isotope composition of aqueous and solid-phase Fe in the Bay of Vidy sediments. Extensive Fe(III) reduction has occurred, resulting in the conversion of nearly all reactive (non-silicate) Fe(III) to a variety of Fe(II)-bearing phases, with mixed Fe valence magnetite being a minor but easily detectable component (0.5–8wt.%). Very little Fe isotope variation was observed in any solid phase Fe components, including magnetite, although significant fractionation was observed between aqueous and solid-phase Fe(II). Because Fe mass-balance was dominated by the solid phase, little net change in δ56Fe values for Fe(II)-bearing components was produced despite clear evidence for DIR. This study provides a basis for interpreting instances in the rock record where DIR was the driving force for Fe(II) production and magnetite formation, yet no significant deviations in δ56Fe values were preserved. A key implication of the results is that Fe isotope homogeneity is not sufficient to rule out a biological mechanism for magnetite formation, and this should be taken into account when examining the Precambrian rock record.

Spatial extent and ecotoxicological risk assessment of a micropollutant-contaminated wastewater plume in Lake Geneva

In this study, the spatial extent of a wastewater-influenced water mass (plume) originating from a wastewater treatment plant outlet in Vidy Bay (Lake Geneva) was monitored by two manned submersibles from June to August 2011. The main goal was to assess whether micropollutants in the wastewater mass cause an ecotoxicological risk to the aquatic environment, and to determine how far the zone of risk extends beyond the wastewater outlet. Real-time measurements of elevated electrical conductivity were used as a proxy to indicate the presence of wastewater-influenced water. Conductivity was highest in immediate proximity to the wastewater outlet, though if all measurements obtained over the duration of the sampling campaign were integrated, elevated conductivity extended over an area of at least 1 km2 surrounding the outlet. Additionally, water samples were collected within and outside Vidy Bay, and were analyzed for 39 micropollutants (pharmaceuticals, pesticides, and corrosion inhibitors). Micropollutant concentrations were generally in the low ng/L range, though for some substances >100 ng/L was measured. The concentrations of most pharmaceuticals, which are primarily wastewater-derived, decreased with decreasing conductivity and with increasing distance from the wastewater outlet. Pesticide concentrations, in contrast, were homogeneous throughout Vidy Bay and the lake. An ecotoxicological risk assessment based on the cumulative risk exerted by all measured substances indicated that the wastewater caused a zone of potential ecotoxicological risk that extended well into the deep lake and in the direction of a downstream drinking water intake.

Deployment of a dynamic penetrometer from manned submersibles for fine‐scale geomorphology studies

Dynamic penetrometers reveal information about seafloor strength, stratification, stability, and sediment remobilization. However, positioning is often limited to a range of meters making it difficult to target small‐scale geomorphologic features. Deployments from submersed vessels (manned or remotely operated) can extend the possibilities of in situ geotechnical surveying in areas of complex bathymetry. The lightweight dynamic penetrometer Nimrod was modified to enable its deployment from the MIR submersibles, and was deployed during two dives in the Rhône Delta and Vidy Bay (both Lake Geneva). In the Rhône Delta, five positions at the floor/levee complex of a submarine canyon were sampled with ~1 m spacing, with good reproducibility of the results. In Vidy Bay, so‐called pillow‐hollow structures on the lake bottom with dimensions of about 50 cm were targeted. At both sites, the penetrometer was released from a height of about 1‐2 m above the lake bottom using the starboard robotic arm of the MIRs leading to impact velocities of ~ 3 m s−1. The probe reached penetration depths of up to 80 cm with maximum decelerations of up to 2.9 g. Stratification in the deceleration versus penetration depth profiles hinted at recent sediment remobilization processes. Pressure transducer results of the probe were suitable to determine water depth, and estimate trends of excess pore pressure. This article describes the modification of the dynamic penetrometer Nimrod for deployments from the MIR submersibles, assesses the deployment performance, validates the results, and gives an outlook on the application of this technique and the results.

Micropollutant Dynamics in Vidy Bay—A Coupled Hydrodynamic-Photolysis Model to Assess the Spatial Extent of Ecotoxicological Risk

The direct discharge of effluent wastewater into Vidy Bay (Lake Geneva) results in the formation of an effluent plume with locally high concentrations of wastewater-derived micropollutants. The micropollutant hotspots above the wastewater outfall present a potential ecotoxicological risk, yet the spatial extent of the plume and the associated ecotoxicological risk zone remain unclear. This work combines the two main processes affecting the spreading of the plume, namely dilution of micropollutants due to mixing and degradation by photolysis, into a coupled hydrodynamic-photolysis model, with which we estimated the spatial extent of the risk zone in Vidy Bay. The concentration of micropollutants around the wastewater outfall was simulated for typical wind scenarios and seasons relevant to Vidy Bay, and the resulting ecotoxicological risk was evaluated. Specifically, we determined the direct and indirect photolysis rate constants for 24 wastewater-derived micropollutants and implemented these in a hydrodynamic particle tracking model, which tracked the movement of water parcels from the wastewater outfall. Simulations showed that owing to thermal stratification, the zone of ecotoxicological risk is largest in summer and extends horizontally over 300 m from the outfall. Photolysis processes contribute to reducing the plume extent mainly under unstratified conditions when the plume surfaces. Moreover, it was shown that only a few compounds, mainly antibiotics, dominate the total ecotoxicological risk.

Current variability in a wide and open lacustrine embayment in Lake Geneva (Switzerland)

Field measurements and numerical simulations were used to determine the effects of dominant meteorological conditions on the hydrodynamics of a wide (aspect ratio ~2), relatively deep (seasonally stratified) and open lake embayment (Vidy Bay, Lake Geneva). A three-dimensional hydrodynamic model (Delft3D-FLOW) was employed to simulate flow in the lake. High-resolution maps of wind, temperature and humidity (over the lake) were applied as input to drive the model. Because wind was the main force driving flow in the lake, currents in the embayment were investigated systematically for different wind conditions and seasonal stratification. Satisfactory model validation was achieved using drifter and moored measurements within the embayment. Markedly different circulation patterns were measured within the embayment, with the transition from one pattern to another occurring abruptly for small changes in wind direction. These distinct patterns resulted from relatively small changes in the large gyre of Lake Geneva's main basin, especially the angle between the current in front of the embayment and the embayment shoreline. The boundary between the embayment and the pelagic zone was defined by the largest gyre within the embayment. This study shows that, (i) in a large lake, complex current patterns can occur even within a minor embayment, and (ii) that these patterns can transition rapidly over a small range of wind directions. Near-shore gyre can occur for lengthy periods, which has implications for flushing of discharges within the embayment.

Substance flow analysis as a tool for mitigating the impact of pharmaceuticals on the aquatic system

Pharmaceuticals constitute an important environmental issue for receiving waters. A holistic approach, taking into consideration the sources of these compounds (hospitals, domestic use), discharges (wastewater effluent, combined sewer overflows) and related risks to the environment, is therefore needed to develop the best protection strategy.The substance flow analysis (SFA) approach, applied, for example, to the city of Lausanne, Switzerland, is an ideal tool to tackle these issues. Four substances were considered: one antibiotic (ciprofloxacin), an analgesic (diclofenac), and two anti-epileptics (carbamazepine and gabapentin). Consumption data for the main hospital of the city (916 beds) and for the population were available. Micropollutant concentrations were measured at different points of the system: wastewater inlet and outlet (WWTP), combined sewer overflows (CSO) and in the receiving waters (Vidy Bay, Lake Geneva). Measured and predicted concentrations were in agreement, except for diclofenac, for which analytical uncertainties were expected. Seven different scenarios were considered (supplementary treatment at the WWTP, at the hospital or at both places, etc.). Based on the results obtained, the supplementary treatment at the WWTP decreases the load of pharmaceuticals reaching surface water by a factor between 2 and 27, depending on the compound and on the technique. The treatment at the hospitals only influences the amount of ciprofloxacin reaching the environment and decreases the release by one third. The contribution of CSO to surface water pollution is low compared to that of the WWTP for the selected compounds. Regarding the risk for the receiving waters, ciprofloxacin was found to be the most problematic compound, with a risk quotient far above 1. In this particular case, a treatment at the WWTP is not sufficient to reduce the risk, and additional measures at the CSO or at the hospital should be considered. SFA is an ideal tool for developing the best strategy for pharmaceutical elimination, but its application depends on data availability and local conditions.

Water Pollution History of Switzerland Recorded by Sediments of the Large and Deep Perialpine Lakes Lucerne and Geneva

This review addresses the quantification of anthropogenic pollutants in lacustrine sediments by multidisciplinary analyses including: chronostratigraphy using radioisotopes (137Cs) and radiocarbon dates (14C), trace metal analysis, faecal indicator analysis, as well as antibiotic-resistant genes by molecular analysis. Sediment cores from lakes Lucerne and Geneva that are located at a distance of 150 km from each other reveal a synchronous increase in anthropogenic trace metals (Pb, Cu, Zn, and Mn) following the industrial revolution in Europe about 1850. In both lakes, the peak of water pollution by toxic metals due to discharge of industrial wastewaters was reached in the middle of the twentieth century. During the second part of the twentieth century, both sites show a decrease in metal pollution following the implementation of wastewater treatment plants. On the contrary, the Vidy Bay of Lake Geneva where the treated wastewaters from the city of Lausanne are released since 1964 points out a dramatic increase in trace metal deposition. Later, a high increase in organic matter deposition, in bacteria (Escherichia coli and Enterococcus faecalis) activity as well as antibiotic-resistant genes and bacteria occurred into the bay, simultaneously with the eutrophication of the large and deep perialpine lakes in the 1970s due to excessive external nutrient loading.