Bay Leaf Extract Research Articles

Syzygium Polyanthum Extract as A Therapy for Dyslipidemia

Dyslipidemia is a metabolic disease characterized by an increase in total cholesterol, low density lipoprotein cholesterol, triglycerides, or elevated levels of high density lipoprotein cholesterol. Dyslipidemia is one of the main factors that cause atherosclerosis caused by coronary heart disease (CHD), stroke, and peripheral vascular disease that is incorporated in cardiovascular disease (PKV). Factors that cause dyslipidemia in Indonesia are people who need foods low in fiber and high fat. Traditional therapy is still one option that is still being taken into account in Indonesia. The use of herbal therapy as a therapy for dyslipidemia can be one way out with the use of Syzygium polyanthum or often known as bay leaf. From several studies reported, bay leaf extract has substance flavonoids, tannins, saponins and vitamins C, B3, A, and E which in some studies have been linked to studies of fat in the body. Based on this, bay leaf can be a potential alternative for the development of dyslipidemia to reduce mortality from cardiovascular disease.

The Effects of Different Concentrations of Bay Leaf Extract on Shelf Life of Preserved Scad Fish

Scad fish (Decapterus kurroides) is the most productive fish species. To provide the added value, fishermen can process scad fish into various forms of processed products, such as preserved fish, which extend the shelf life. In order to extend the shelf life of canned fish, natural preservatives could be used that contain antimicrobial agents, such as bay leaves. The present study aimed to determine the effects of bay leaf extract in different concentrations on the shelf life of preserved scad fish. In the present study, the completely randomized design was used as an experimental research method. The treatments given differed in terms of the concentration of the bay leaf extract. The groups were treated as the scad fish without bay leaf extract (A), scad fish soaked in 6% of bay leaf extract (B), scad fish soaked in 7% of bay leaf extract (C), scad fish soaked in 8% of bay leaf extract (D), and scad fish soaked in 9% of bay leaf extract (E). The analyzed parameters included the total plate count, pH, and water level test using Analysis of Variance (ANOVA). The supportive parameters observed included an organoleptic test. The results indicated that the lowest total bacterial count from the beginning to the end of the experiment (18 hours) occurred on treatment C (7% of bay leaf extract addition), which was 1.54 × 103 to 5.85 × 106. Regarding the water level test from the beginning to the end of the experiment, treatment A (control) was not significantly different from other treatments. The difference in the concentration of bay leaf extract on scad fish effectively inhibited the growth of bacterial colonies. In conclusion, adding bay leaf extract to scad fish could inhibit bacteria for up to 12 hours. Treatment C (7% of bay leaf extract) gave the best results since this concentration level could inhibit the bacteria on scad fish.

Functional Characteristics Improvement of Edible Film through Addition of Gambier and Bay Leaf Extract

Background: In this study, the composition of the functional edible film focused on local materials was examined. However, the production of the edible film with strong capability as an antioxidant and antimicrobe has not been successful. Therefore, the incorporation of one or more functional compounds such as gambier and bay leaf extract into canna starch is a possible solution. Objective: These compounds work in synergy to improve the functional characteristics of edible film. Furthermore, the film should have mechanical characteristics which satisfy the Japanese Industrial Standard (JIS) (1975), i.e. strong category. Methods: This study examined the effects of gambier and bay leaf extract addition on edible film characteristics, by implementing a completely randomized design method. The two factors examined were gambier (1.0, 2.0 and 3.0 percent, w/v) and bay leaf extract (0.0, 3.0 and 6.0 percent, w/v) addition. Results: The parameters observed include mechanical (thickness, elongation percentage, water vapor transmission rate) and functional characteristics (antioxidant and antibacterial activity). The results showed that thickness, elongation percentage and water vapor transmission rate of the functional edible film were 0.18-0.27 mm, 7.33-9.00% and 30.43-46.07 g.m-2.d-1, respectively. While antioxidant and antibacterial activities (value of inhibition diameter) were 23.24-40.58 mg.mL-1 and 1.33- 1.83 mm, respectively. Conclusion: The edible film produced in this study had a strong category of antioxidant activity with a thickness that satisfied the JIS 1975 standard.

Syzygium polyanthum Extract as a therapy for dyslipidemia

Dyslipidemia is a metabolic disease characterized by an increase in total cholesterol, low density lipoprotein cholesterol, triglycerides, or elevated levels of high density lipoprotein cholesterol. Dyslipidemia is one of the main factors that cause atherosclerosis caused by coronary heart disease (CHD), stroke, and peripheral vascular disease that is incorporated in cardiovascular disease (PKV). Factors that cause dyslipidemia in Indonesia are people who need foods low in fiber and high fat. Traditional therapy is still one option that is still being taken into account in Indonesia. The use of herbal therapy as a therapy for dyslipidemia can be one way out with the use of Syzygium polyanthum or often known as bay leaf. From several studies reported, bay leaf extract has substance flavonoids, tannins, saponins and vitamins C, B3, A, and E which in some studies have been linked to studies of fat in the body. Based on this, bay leaf can be a potential alternative for the development of dyslipidemia to reduce mortality from cardiovascular disease.

AKTIVITAS EKSTRAK ETANOL DAUN SALAM [Syzygium polyanthum (Wight) Walp.] SEBAGAI PENGHAMBAT ENZIM α-GLUKOSIDASE DAN STUDI SECARA IN SILICO

Diabetes Mellitus (DM) is a degenerative disease that can cause various complications such as cardiovascular, stroke, and hypertension. Antidiabetic drugs that are commonly circulated in the market, have side effects that can harm other organs such as liver, kidneys and other organs. Bay leaves have properties as inhibitors of the α -glucosidase enzyme in vitro and in vivo. The aims of this study was determine the activity of ethanolic extract of bay leaves by ultrasonic extraction method in inhibiting the α -glucosidase enzyme in vitro and in silico. Bay leaves were extracted using 70% and 96% ethanol by ultrasonic method for 15 minutes, and filtered and concentrated using a rotary vacuum evaporator 4 times. The extract obtained was tested for phytochemical content and the activity of α -glucosidase inhibitory assay. The extract with the best IC50 value was then separated using column chromatography (SiO2, dichloromethane, methanol) and produced 7 fractions and tested for α -glucosidase enzyme inhibition. The best fraction (fraction 6) was analyzed by Liquid Chromatography Mas Spectra (LCMS-MS). The results showed that bay leaf extract containing alkaloids, flavonoids, tannins, steroids, phenols, and fraction-6 had the best IC50 of 30.82 ppm. The results of LCMS-MS analysis of chemical compounds as inhibitors of the α -glucosidase enzyme were delphinidin (m/z 303) and had a rerank score of -75.59. In the in silico test using PLANTS docking software.

Phytochemical Screening Mango Bacang Leaf Extract (Mangifera foetida L) and Salam Leaf Extract (Syzygium polyanthum)

Bacang mango leaves and bay leaves are plants that have been used for a long time, even bay leaves themselves are often used as cooking ingredients. Mango Bacang leaves and bay leaves contain alkaloids, phenols, tannins, flavonoids, steroids, saponins, triterpenes, polyphenols as well as essential oils. So this research was conducted to develop the use of mango Bacang leaves and bay leaves as natural medicine. The sample was determined and identified in the laboratory of the Faculty of Mathematics and Natural Sciences, Riau University. Furthermore, the mango Bacang and bay leaves were macerated using 96% ethanol and extracted until a thick extract was obtained which would then be tested for phytochemical screening on the Bacang mango leaf extract and bay leaf extract. The results obtained that the extract of mango Bacang leaf and bay leaf extract were positive for containing flavonoid compounds, saponins and tannins so that they could be developed to be used as natural remedies.

Cardioprotectant Effect Of Bay Leaf Extract (Syzygiumpolyanthum) On Myocardium Thickness Of Rat Heart

High salt diet and prednisone administration will increase blood pressure which chronically causes heart muscle remodeling so that it appears macroscopically as left ventricular hypertrophy. Bay leaf extract contains flavonoids which can lower blood pressure and decrease left ventricular thickening. To determine the effect of bay leaf extract (Syzygiumpolyanthum) on the left ventricle thickening of rat heart (Rattus norvegicus wistar strain) in hypertensive model. True experimental post-test only controls group design with simple random sampling technique. The subjects of this study were 25 male Wistar rats aged 2-3 months weighing 150-200 grams. Rats were induced with prednisone 1.5 mg/ KgBW / day in 2% NaCl for 6 weeks followed by administration of bay leaf extract at a dose of 70 mg/ KgBW, a dose of 140 mg/ KgBW, and a dose of 280 mg/ KgBW. Data analyzed by One Way ANOVA, Post Hoc Bonferroni, followed by Linear: One Way ANOVA test showed significant differences between treatments (p = 0.000). The Bonferroni Post Hoc test concluded that there were significant differences in the treatment of the P2 and P3 groups. Linear regression test obtained R square 0.663 and obtained the equation Y = 1965.838 - 1.275X. The extract of bay leaf (Syzygiumpolyanthum) has an effect on decreasing the left ventricle thickening of the white male rat heart (Rattus norvegicus wistar strain) hypertension model.

Uji Daya Hambat Ekstrak Daun Salam (Syzygium polyanthum wight) Terhadap Pertumbuhan Bakteri Porphyromonas Gingivalis Secara In Vitro

Chronic periodontitis is the most common type of periodontitis. The main cause of chronic periodontitis is bacterial colonization of plaque. Porphyromonas gingivalis is the main pathogen and has the highest prevalence of chronic periodontitis. Bay leaf extract (Syzygium polyanthum wight) is believed to have the pharmacological effect that can be used as antibacterial. The purpose of this research was to determine the inhibitory of bay leaf extract on the growth of Porphyromonas gingivalis in vitro. The method of this research was experimental laboratories with posttest only control group design. There were 30 samples divided into 6 groups, namely bay leaf extract concentration of 2.5%, 5%, 10%, 20%, 40% and Dimethyl Sulfoxide (DMSO) as a control. Inhibitory test using the Kirby-Bauer method with a paper disc on Mueller Hinton medium agar. Inhibition zone that created around paper disc was measured with the sliding caliper. The results were analyzed with One Way Anova and Post Hoc LSD test. The results of this research showed that the mean of bay leaf extract 40% had the largest inhibition zone that was 7,6 mm and the lowest formed by bay leaf extract 2,5% that was 1,94 mm. One Way Anova test showed that there was a significant difference among the group (p<0,05). The conclusion of this research there was significant difference inhibition among 2,5%, 5%, 10%, 20%, and 40% concentration of bay leaf extract on the growth of Porphyromonas gingivalis in vitro. The higher concentration of bay leaf extract resulting the greater inhibition zone.

DAYA HAMBAT BERKUMUR EKSTRAK DAUN SALAM (SYZYGIUM POLYANTHUM) TERHADAP PEMBENTUKAN PLAK PADA MAHASISWA JURUSAN KEPERAWATAN GIGI DI BUKITTINGGI

Bay leaves (Syzgium Polyanthum) contain flavonoids, tannins and essential oils which act as antibacterial. Plaque contains bacteria that stick closely to the surface of the teeth which can cause disease in the hard tissues of the teeth and the supporting tissues of the teeth. Plaque control is carried out mechanically and chemically, on of which is gargling bay leaf extract. Plaque measurement using the plaque index. The research objective was to determine the inhibition of gargling of bay leaf extract against plaque formation. This study used a quasi experimental method wth a post test control group design. The sampling technique was simple random sampling, the number of sample was determined Fredere’s formula, the sample devided into 3 groups, each group conssiting of 9 respondents.Group 1 rinsed with bay leaf extract, group 2 rinsed with antiseptic mouthwash and group 3 rinsed with aquadest. Data were tested by using Annova test. The result showed that the average plaque index after rinsing the bay leaf extract was 1.056, the average plaque indexgargling antiseptic mouthwash was 2.478, and the average plaque index after gargling with aquadest was 2167.The significance value is 0.178 and f count > f table (6.238> 3.403). The conclusion was garglingbay leaf extract wwas more effective in inhibiting plaque formation. It is recommended to use bay leaf extract as a mouthwash to maintain oral health.

ANTIBACTERIAL EFFECT OF EUGENIA POLYANTHA WIGHT WITH CORYNEBACTERIUM DIPHTHERIAE NON-TOXIGENIC

Introduction: Corynebacterium diphtheriae is a bacteria that causes diphtheria. In recent years, the bacterium is resistant to antibiotics. Eugenia polyantha wight might be a suitable antibiotic alternative as it has antibacterial effect against Staphylococcus aureus. The content of the antibacterial compound in Eugenia polyantha wight at specific concentrations is expected to be able to kill the Corynebacterium diphtheriae. Purpose: This study aimed to examine the antibacterial effect of Eugenia polyantha wight to Corynebacterium diphtheriae non-toxic. Method: Bay leaf extract (Eugenia polyantha wight) made with maceration method. This research is an experimental study conducted in vitro using a microdilution test on 96 well plates then count value with the streaking method to find out MBC. Results: bactericidal power was quite effective at the concentration of 40%, while at the concentration of 80%-100% has very strong bactericidal power that could destroy the growth of bacteria 99.9%. The higher the concentration of bay leaves extract, the higher the bactericidal power of bacteria Conclusion: Eugenia polyantha wight has an antibacterial effect against Corynebacterium diphtheriae non-toxic.

EKSTRAK DAUN SALAM DALAM MENINGKATKAN EKSPRESI FIBROBLAST GROWTH FACTOR PADA ULKUS TRAUMATIK RONGGA MULUT

Background: Traumatic ulcers are lesions of the oral mucosa caused by mechanical, chemical, and temperature trauma. Traumatic ulcer treatment aims to reduce inflammation, accelerate the healing process, and reduce pain. Bay leaf has anti-inflammatory pharmacological activities. This study aims to determine the effect of bay leaf extract on the expression of fibroblast growth factor in traumatic ulcer oral mucosa in Wistar rats. Method: This is an experimental research with a post-test only control group design, consisting of four groups with 20 sample of male Wistar rats. All animals were injured using a hot burnisher attached to the labial mucosa for 1 second. The gel was applied twice daily until the 3rd and 5th day after the ulcer has formed. Rat mucosal tissues were analyzed by immunohistochemical staining for FGF expressions. Statistical analysis using SPSS Mann-Whitney test. Result: The results showed the mean of cells expressing FGF in the bay gel extract group on the 3rd and 5th days were 8.4 ± 0.8, and 13.6 ± 0.5. Mann Whitney test results showed P<0.05 there is a significant differential expression of FGF traumatic ulcer oral mucosa of experimental animals on days 3 and 5 compared control group. Conclusion: Bay leaf extract increased FGF expression on days 3 and 5

The Relationship Between Lipoprotein (A) And Lipid Profile In Patients Treated With Bay Leaf Extract [Syzygium Polyanthum (Wight) Walp] In Patients Dyslipidemia

Background: Increased Lp(a) accelerates atherosclerosis by means of cholesterol deposits in tunica. The purpose of the study was to assess the relationship of decreased Lp(a) with lipid profile in patients with dyslipidemia given bay leaf extract [syzygium polyanthum (Wight) Walp] treatment. Method: Thirty subjects were divided into 2 groups: group I received salam leaf extract therapy 2 x 200 mg and group II received salam leaf extract therapy 2 x 300 mg per day capsules for 30 days randomly selected and double blind. Examination of Lp(a), total cholesterol, LDL-C, HDL-C and TG before and after research. The manufacture of bay leaf extract is done by maseration, the extraction process using ethanol 70%, then the extract is inserted in the capsule. Result: In groups I and II there were significant differences in variable Lp(a) and lipid profiles after treatment except HDL-C. There is a significant correlation between Lp(a) and LDL at salam leaf extract therapy doses of 2x200 mg per day. Conclusion: Decrease in Lp(a) is significantly correlated with decreased LDL-C. Salam leaf extract therapy can predict decreased cardiovascular risk in patients with dyslipidemia.

Relationship Between Triglycerides And Glucose Index (TyG) And Lipid Profile In Patients With Dyslipidemia Treated With Bay Leaf Extract [Syzygium Polyanthum (Wight) Walp

Background: Insulin Resistance (IR) causes a metabolic disorder including hyperglycemia, high blood pressure, and dyslipidemia. The purpose of the study was to assess the relationship of Triglycerides and Glucose Index (Tyg) decline relationship with lipid profile after bay leaf extract treatment in dyslipidemia patients. Method: Fifteen dyslipidemia patients were administered 2x200 mg salam leaf extract capsules for 30 days. Criteria dyslipidemia is defined as one of the following: total cholesterol level ≥ 240 mg/dL, triglyceride levels ≥150 mg/dL, LDL cholesterol levels ≥140 mg/dL, HDL cholesterol levels <40 mg/dL, or the use of lipid-lowering drugs. Blood samples lipid profile for biochemical tests were drawn in the morning after fasting 12 h. Anthropometric BMI and waist circumference measures were taken using standard procedures. The TG/HDL-C ratio was calculated by dividing the serum concentration of TG by HDL-C measured in mg/dL. TyG index was calculated based on formula: Ln [TG (mg/dL) x FG (mg/dL). Result: After 30 days of salam leaf extract treatment, there was a significant correlation of TyG reduction with parameters TC, LDL-C, TG, TG/HD ratio (p: 0.025*, p: 0.001*, p: 0.013*, sequentially) Conclusion: There is a significant correlation between Tyg reduction with TC, TG, and TG/HDL, which leads to a decrease in cardiovascular risk in dyslipidemia patients.

The Effect of Salam Leaf Extract (Syzygium polianthum) Against Cholesterol Regulation in Hypercholesterolemic Model White Rats

Cholesterol is an important precursor that plays a role in the production andregulation of various sterol group compounds, especially steroid hormonecompounds, androgen hormone compounds, cortisol compounds and estrogencompounds. This herb is known to have the effect of improving glucose regulationby increasing glucose intake into cells and tissues. Its ability to improve cell andtissue metabolism is mediated by the content of secondary metabolite compounds.This ability is believed to have the potential to improve cholesterol regulation. Bayleaf extract is effective in lowering cholesterol levels through regulation of Acetyl CoAproduction.

Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan

Diabetes mellitus is a chronic condition that disturbs the body's bloodsugar regulation. Bay leaves contain entirely various secondary metabolites,where this plant is rich in flavonoids, alkaloids, terpenes and glycosides. Thisstudy aims to assess the effect of bay leaf extract (Syzygium polyanthum) onblood sugar levels and the expression of GLUT4 protein in muscle tissue. A totalof 30 white rats (Rattus norvegicus) Wistar strain obtained from the EurekaResearch Laboratory (Palembang, Indonesia) weighing between 200 - 250 grams.Bay leaf simplicia was obtained from the Tawangmangu Herbal Research Center,Karanganyar, Indonesia. After 1 week of adaptation, the mice were randomlydivided into the following six groups, each containing 5 animals: Normal controlgroup, diabetes group (negative control), diabetes + metformin group (Met; 45mg / kg), Diabetes + BLE (75 mg/kg), diabetes + BLE group (150 mg / kg) anddiabetes + BLE group (300 mg/kg). Alloxan-induced white rats showed a verysignificant increase in blood sugar levels, where the use of the drug metforminwas able to reduce blood sugar levels significantly even though they had notreached the target blood glucose target of less than 200 mg / dL. The treatmentwith bay leaf extract was able to reduce blood sugar levels significantly. Theadministration of metformin drugs or bay leaf extract showed the ability toincrease the level of GLUT4 protein. In conclusion, bay leaf extract affectsreducing blood sugar levels in diabetes mellitus white rats by increasing glucoseintake to cells and tissues.

Effect of Salam leaf extract (Syzygium Polyanthum) to serum IL-10 levels on Acute Coronary Syndrome in Wistar Strain Mice Model

Background Coronary Heart Disease (CHD) is one of the leading causes of death in developed and developing countries. Syzygium polyanthum leaf (daun salam) are reported to have anti-inflammatory, antioxidant, antidiabetic, antimicrobial, antihypertensive, antitumor, antidiarrheal, acetylcholinesterase inhibition, and lipase inhibitory activity. The purpose of this study was to prove the effect of bay leaf extract (Syzygium polyanthum) on serum interleukin (IL)-10 levels in the heart of Wistar Strain Mice Model of Acute Coronary Syndrome (ACS). Methods This is an experimental study to compare the IL-10 level in 32 ACS mice which were divided into 8 groups. Serum IL-10 values were collected and analyzed according to the corresponding group with and without bay leaf extract administration, on day 1,4,7, and 14. Result: There is an IL-10 value increasing with the duration of examination following the onset of ACS induction. The highest average IL-10 value was found on the 14th day, in the two groups. On the same day, it was seen that the treatment group had a higher IL-10 value compared to the control group (p<0.001). The result showed that the concentration of IL-10 was significantly greater among the treatment group, which reveal the potential benefit of bay leaf extract in patients with ACS. Conclusion: There was found a significant difference of serum IL-10 between Wistar Strain Mice Model of ACS that was given S. polyanthum leaf extracts. S. polyanthum leaf extract has quite extensive benefits in the cardiovascular system.

In Silico Study and Bioactivity of Flavonoid Extract Syzygium polyanthum (Wight) Walp. Leaves Against Salmonella typhi

Salmonella typhi bacteria cause complications of bleeding in the intestine or intestinal perforation. However, Salmonella typhi bacteria will become resistant to chloramphenicol due to the formation of plasmids that produce the chloramphenicol acetyl transferase (CAT) enzyme which works to activate chloramphenicol. So, the search for active groups of natural compounds which are expected to have the work of deactivating the CAT enzyme. One of them is a flavonoid compound. The flavonoid group is widely used as an antimicrobial. This research was aimed at the extraction of bay leaf flavonoids. The presence of flavonoids was proven by phytochemical tests, namely a yellow color change with a slightly acidic amyl alcohol solvent - Mg metal and strengthened by the scanning results of the maximum wavelength on the band 1 407 nm and band 2 338 nm which is a special feature of flavonoid backbone. The results of GC-MS analysis obtained 4 flavonoid compounds from bay leaf extract which have an abundance above 1% and qualification above 90%: 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, alpha- tocopherol, beta-tocopherol, and pyragallol. The correlation between the results of the inhibitory test against Salmonella typhi at a concentration of 100 ppm with the results of docking tocopherol - 3CLA receptors which have an affinity bond energy / ∆G binding = 7.40 kcal / mol, indicates that the bay leaf flavonoid extract can inhibit the formation of chloramphenicol acetyl transferase (CAT).

The Effect of Bay Leaf Extract (Syzygium polyanthum) on Reducing Overexpression of Matrix Metalloproteinases-13 in Acute Coronary Syndrome

AIM: The objective of the study was to determine the inhibitory effect of bay leaf (Syzygium polyanthum) on matrix metalloproteinases (MMPs) (MMP-9 and MMP-13) inhibitory effect in surgery-induced acute coronary syndrome (ACS) rat model.
 METHODS: This study was experimental animal study. Thirty-two surgery-induced ACS Wistar rats (Rattus norvegicus) divided into 16 bay leaf extract (treatment) group and 16 control groups were sacrificed on days 1, 3, 7, and 14 after induction. Expressions of MMP-9 and MMP-13 were determined by immunohistochemistry and its scoring was assessed by two-blinded experienced pathologists. The immunohistochemical scoring of MMP-9 and MMP-13 between groups was analyzed using independent sample t-test and within groups by using one-way ANOVA test.
 RESULTS: The expression of MMP-13 in treatment group was decreased compared to control group on days 7 and 14 (p = 0.001 and p = 0.015, respectively). Compared to day 1, the reduction expression of MMP-13 was significant in both control group and treatment group (p = 0.009 and p = 0.003, respectively). There were no significant changes in expression of MMP-9 between groups, and within control group and treatment group.
 CONCLUSION: Bay leaf can reduce the overexpression of MMP-13 in surgery-induced ACS rat model. Bay leaf extract can be considered to be given as an adjuvant to prevent cardiovascular adverse event and adverse cardiac remodeling post-ACS.

BAY LEAF (SYZYGIUM POLYANTHUM) EXTRACT GEL EFFECT ON TNF- Α EXPRESSION IN TRAUMATIC ULCERS HEALING PROCESS

Background: Traumatic ulcers were injuries that occur to the epithelium and basement membrane, due to physical, mechanical, or chemical trauma. The healing process of traumatic ulcers was divided into three main phases, namely inflammatory, proliferation, and maturation phase. TNF-α as a proinflammatory mediator worked 2-3 days which later would be replaced by an anti-inflammatory mediator. This research aimed to determine the impact of bay leaf extract gel, which had a very high anti-inflammatory content, on TNF-α expression in accelerating the ulcers healing process. Method: This research method was an in vivo experimental laboratory research using a post-test only control design. The sample consists of 20 rats divided into four groups, namely, the control group on day 1, control groups on day 3, treatment groups on 1st day, and treatment groups on 3rd day. Rats were made traumatic ulcers on the lower lip mucosa and were given bay leaf extract gel in the morning and evening. The tissue was then made into a histological preparation and given immunohistochemical staining to see TNF-α. Result: The results showed the mean of cells expressing TNF-α in the bay leaf gel extract group on the 1st and 3rd days has P> 0.05. Independent T-test on 1st day dan 3rd group showed a significant difference in TNF-α expression in the control and treatment groups. Conclusion: The conclusion obtained in this study was bay leaf extract gel had a significant effect on the expression of TNF-α in the traumatic ulcers healing process.

Oxidation Lipid on Fish Cake Catfish (Pangasius sp.) Dissolved in The Extract of Bay Leaf (Eugenia polyantha)

Catfish (Pangasius sp.) As a source of animal protein. Fish catfish have a thick white meat so that it can be processed into a wide range of diversified products. The process of fried fish cake can result in fat or autooxidation process. Autooxidation can be inhibited by the presence of antioxidant compounds. One source of natural antioxidants are bay leaf Eugenia polyantha.This research was carried out by experimental methods and the use of experimental design of randomized Complete Design (RAL). RAL is used when the material and condition of the experiment are homogeneous. This research consists of four concentrations of extract treatment bay leaf, namely 0%, 10%, 20%, 30%. Fish cake has been fried done soaking for 30 minutes. Water content showed no significant difference (p> 0.05) at 24 hours to 28, this indicated that the addition of bay leaf extract did not affect the moisture content during storage. The high water content at 0 hour is caused by the process of soaking the fish cake in the extract solution. The highest value is shown by the concentration of 30% at the hour to 0 that is 52.78%, this indicates that the fish cake by soaking can meet the quality score SNI 7757:2013 which is a maximum of 60%. The fat content in the fish cake continues to decrease, indicating the occurrence of oxidation during storage. Levels of TBA in fish cake continue to increase that can be slowed by the extract of bay leaves with a concentration of 30%.