Basic Structural Units Research Articles

Impact of SnO2 doping on spectroscopic characteristics of the SnO2-Na2O-CoO-B2O3 borate glass matrix

The role of tin oxide (SnO2) on the structure-optical features of borate glasses containing low cobalt oxide impurities was studied. The study was carried out on a glass system [x SnO2 – (80-x) B2O3 – 19.5 Na2O – 0.5 CoO]; x = 0.0, 0.5, 1.0, 2.0 and 3.0 mol%. Glass synthesis was carried out by the melt-quench technique. Structure and optical absorption spectroscopy were carried out to explore the optical transitions of Co cations inside the present glass matrix. The obtained results indicate that, with the further addition of SnO2 content, the molar volume decreased from 31.675 cm3/mol to 29.147 cm3/mol. FTIR studies indicated the basic structural units of trigonal BO3 units and BO4 tetrahedra. Herein also, additional contents of tin oxide increased the nonbridging oxygen contents. The reason behind such increment is attributed to the existence of Sn4+ ions in octahedral coordination acting as network modifiers. Optical absorption spectra indicated the existence of Co ions in both Co2+ and Co3+ oxidation states. Moreover, the existence of significant SnO2 concurred with the significant blue shift in the visible absorption bands and red shift in near infrared absorption bands. The positions absorption bands of Co ions were analyzed in the context of ligand field parameters determinations. The obtained data of the crystal field splitting parameter (10Dqt) showed decreased values from 3451 cm−1 to 3364 cm−1 with more SnO2 addition. While Racah parameter B values were also, attained showing increased values from 967 cm−1 to 985 cm−1 when additional SnO2 is added. Further, the impacted role of SnO2 addition on the structural and optical properties of the present glasses was finally estimated.

Topological states in Penrose-square photonic crystals

Topological edge states (TESs) and topological corner states (TCSs) in photonic crystals (PCs) provide an effective way to control the propagation and localization of light. The topological performance of integrated photonic devices can be improved by introducing the basic structural unit of photonic quasicrystals (PQCs) into PCs. However, the previous works arranged the basic structural unit of Stampfli-type and 12-fold Penrose-type photonic quasicrystals into triangular lattices, which have a complex structure and allow light to only propagate around 60° or 120° corners, limiting their applications. In this paper, a Penrose-square PC is proposed, which realizes both TESs and TCSs, and light successfully propagates around 90° corners. This work may reduce the difficulties encountered in the preparation of topological photonic crystals (TPCs) structured by arranging the basic structural units of PQCs periodically. It also provides a new, to the best of our knowledge, platform for studying TPCs and new ideas for improving the performance of integrated photonic devices.

A Rare-Earth-Based Borate Optical Crystal with Enlarged Dihedral Angles of Distinct [BO3] Groups Exhibiting a Wide UV Transparent Range.

Borates, as advanced optical materials, have garnered wide interest due to their diverse structural configurations and great potential for applications in the ultraviolet (UV) regions. Herein, we synthesized a new rare-earth borate crystal, namely, K2NaYB2O6, which is classified as one of the ABReB2O6 compounds, where A and B represent alkali metal and Re denotes rare-earth metal. K2NaYB2O6 adopts in the monoclinic space group P21/c (No. 14), showcasing a three-dimensional (3D) framework composed of a planar triangular configuration of [BO3] units and distortive [YO7] polyhedra. Notably, both dihedral angles between distinct [BO3] units reach 79.6°, which represents an unprecedented structural feature in monoclinic ABReB2O6-type crystals. Moreover, the compound has a short UV absorption edge at around 204 nm, corresponding to a wide band gap of approximately 5.67 eV. Additionally, it possesses a moderate birefringence of 0.028 at 1064 nm. Further analysis utilizing theoretical calculations suggests that the optical behaviors of K2NaYB2O6 are mainly governed by its basic structural unit [BO3] triangles and distorted [YO7] polyhedra. These findings enrich the structure chemistry of rare-earth borates and offer valuable insights for the design of optical crystals in the UV wavelength range.

Insight into the TiO2 on transport behavior and heat transfer of the CaO–SiO2–FeO–MgO system at different basicities: Molecular dynamics simulation and thermodynamics

In order to promote the resource utilization of Ti-containing converter slag and reduce the loss of metallic Ti, it is necessary to clarify the relationship between the oxygen network structure and thermophysical properties of the slag. The present work uses classical molecular dynamics (CMD) simulation to study the effects of basicity (1.0–3.0) and TiO2 content (0%–10 %) on the local structural properties, viscosity, and thermal conductivity of the CaO–SiO2–FeO–MgO–TiO2 (CSFMT) system at 1873 K. The M − O (M = Ca, Si, Fe, Mg, and Ti) bond length calculated based on CMD is consistent with the experimental results, and the [SiO4] tetrahedron and [TiO6] octahedron are the basic structural units of the CSFMT system. The increase in basicity promotes the break of bridging oxygen (BO) Si–O–Si and the formation of non-bridging oxygen Si–O-M. The increase in TiO2 content promotes the formation of Ti–O–Si in the system. The basicity and TiO2 content affect the thermophysical properties of the CSFMT system by promoting and inhibiting the depolymerization of the oxygen network structure, respectively. The diffusion abilities of different atoms in the CSFMT system are in descending order of Fe > Mg > Ca > O > Si > Ti. The viscosity of the CSFMT system decreases with increasing basicity and increases with increasing TiO2 content. Increasing the proportion of BOs can weaken the anharmonicity in the polyhedral structural units and reduce phonon scattering. With increasing basicity and TiO2 content, the effective phonon mean free path decreases and increases, respectively, resulting in a decrease and an increase in the thermal conductivity of the CSFMT system. The changes in the microstructure properties of the CSFMT system can be reasonably explained by the changes in its thermodynamic properties such as enthalpy. It is worth noting that the basicity has a greater effect on the viscosity, while the TiO2 content has a more significant impact on the thermal conductivity of the CSFMT system.

Amorphous molybdenum sulfide nanosheets composed of [Mo3S13]2- active-site motifs for enhancing conversion of Fe3+/Fe2+ in Fenton reaction under neutral condition

Molybdenum disulfide (MoS2) edges have been considered as effective active sites to promote Fenton reaction, whereas the basal plane is inert. It is dauntingly challenging to maximally exposure edge active sites and active the basal plane. Inspired that [Mo3S13]2- clusters is basic structural unit of MoS2 edge, amorphous molybdenum sulfide (a-MoSx) nanosheets composed of [Mo3S13]2- active-site motifs may be an ideal co-catalyst for Fenton reaction. Herein, accordion-like a-MoSx nanosheets were successfully designed and prepared by one step oil-heating method. The as-synthesized a-MoSx exhibited superior activity in co-catalytic Fenton degradation of tetracycline (TC, 10 mg/L) than that of MoS2, which could reach 94.73 % within 5 min under neutral condition. This is attributed to the better Mo-S coordination mode or surrounding environment to accelerate the rate-limiting step of Fe3+/Fe2+ conversion, with DFT calculations indicating for Fe3+ on Mo4+ or S2-/S22- sites in a-MoSx a higher electron aggregation density and lower adsorption energy. Impressively, all the S atoms in a-MoSx have low adsorption energy with H atom, making that the co-catalytic Fenton reaction can be performed under neutral conditions. For a scale-up experiment, 5 L of TC wastewater (50 mg/L) was almost completely degraded after 120 min in the nickel foam/a-MoSx + H2O2 + Fe2+ system.

Epigenetic Histone Modifications H3K36me3 and H4K5/8/12/16ac Induce Open Polynucleosome Conformations via Different Mechanisms

Nucleosomes are the basic compaction unit of chromatin and nucleosome structure and their higher-order assemblies regulate genome accessibility. Many post-translational modifications alter nucleosome dynamics, nucleosome-nucleosome interactions, and ultimately chromatin structure and gene expression. Here, we investigate the role of two post-translational modifications associated with actively transcribed regions, H3K36me3 and H4K5/8/12/16ac, in the contexts of tri-nucleosome arrays that provide a tractable model system for quantitative single-molecule analysis, while enabling us to probe nucleosome-nucleosome interactions. Direct visualization by AFM imaging reveals that H3K36me3 and H4K5/8/12/16ac nucleosomes adopt significantly more open and loose conformations than unmodified nucleosomes. Similarly, magnetic tweezers force spectroscopy shows a reduction in DNA outer turn wrapping and nucleosome-nucleosome interactions for the modified nucleosomes. The results suggest that for H3K36me3 the increased breathing and outer DNA turn unwrapping seen in mononucleosomes propagates to more open conformations in nucleosome arrays. In contrast, the even more open structures of H4K5/8/12/16ac nucleosome arrays do not appear to derive from the dynamics of the constituent mononucleosomes, but are driven by reduced nucleosome-nucleosome interactions, suggesting that stacking interactions can overrule DNA breathing of individual nucleosomes. We anticipate that our methodology will be broadly applicable to reveal the influence of other post-translational modifications and to observe the activity of nucleosome remodelers.

Supramolecular cross-linking affords superelastic and fatigue-resistant cellulose-based nanocomposites with excellent thermomechanical properties and waterproofing performance

Nanocellulose is one of the most abundant renewable resources on earth and is used in many applications due to its excellent physical and chemical properties, and it is an important biopolymer raw material to support human well-being. However, current cellulose-based aerogel nanocomposites are plagued by irreconcilable contradictions between thermomechanical and waterproofing characteristics due to their hygroscopicity. Herein, sustainable insulating cellulose aerogel nanocomposites are prepared from tempo-oxidized bamboo cellulose nanofibrils (CNFs) and polyvinyl alcohol (PVA) with a continuous orientation-free pore architecture formed by CNFs/PVA pre-hydrogel aggregates, which combine to become the basic structural unit for producing superelastic properties. The resulting lightweight CNFs/PVA aerogels (CNPA) with a hierarchical orientation-free pore architecture and density about 5–20 kg m3 achieve an optimal thermomechanical and insulation trade-off, demonstrating temperature-invariant compressibility (–100–500 ℃) and a low thermal conductivity of 38.2 mW m–1 K–1 at 300 ℃. Particularly, the flyweight CNPA retained its structural integrity after more than 106 cycles at ɛ=20 %, 105 cycles at ɛ=50 %, and 104 cycles at ɛ=80 %. Furthermore, CNPA also exhibits robust liquid-repellency with a WCA exceeding 160.3°. This lightweight cellulose-based nanocomposites, although constructed using an intrinsically hygroscopicity nanocellulose-constituent, is simultaneously superelastic, fatigue-resistant, thermomechanical, and waterproofing performance.

Photoredox Catalyzed Synthesis of gem-Difluoroalkenes and Monofluorinated Cyclooctenes via 1,5-HAT Process.

gem-Difluoroalkenes and monofluorinated cycloalkenes have emerged as basic structural units in a variety of bioactive molecules and natural products. Thus, developing straightforward and efficient methods for synthesizing fluorinated alkene compounds is of considerable significance. Herein, we disclose a visible-light-induced defluorination of 2-trifluoromethyl-1-alkene via a 1,5-HAT process using N-alkoxyphtalimides as both radical precursor and potential nucleophile. The mild and stepwise reaction leads to a variety of structurally diverse gem-difluoroalkenes and monofluorinated cyclooctenes with high efficiency, respectively.

OsPPG encodes a pseudouridine-5′-phosphate glycosidase and regulates rice spikelet development

Florets are the basic structural units of spikelets, and their morphogenesis determines the yield and quality of rice grains. However, whether and how pseudouridine-5′-phosphate glycosidase participates in rice spikelet development remains an open question. In this study, we identified a novel gene, OsPPG, which encodes a peroxisome-localized pseudouridine-5′-phosphate glycosidase and regulates the development of rice spikelets. osppg mutants exhibited abnormal sterile lemma, lemma, palea, lodicule, stamens, and pistils; male sterility; shorter panicles; and reduced plant height. OsPPG was found to regulate several OsMADS genes, thereby affecting the morphogenesis of rice spikelets. Furthermore, metabolomics revealed that the OsPPG gene was involved in the decomposition of pseudouridine via the pyrimidine metabolism pathway and may affect the jasmonic acid signaling pathway. These results suggest that OsPPG is a key regulator of rice spikelet development.

Seasonal freeze‒thaw processes impact microbial communities of soil aggregates associated with soil pores on the Qinghai–Tibet Plateau

BackgroundSeasonal freeze‒thaw (FT) processes alter soil formation and cause changes in soil microbial communities, which regulate the decomposition of organic matter in alpine ecosystems. Soil aggregates are basic structural units and play a critical role in microbial habitation. However, the impact of seasonal FT processes on the distribution of microbial communities associated with soil pores in different aggregate fractions under climate change has been overlooked. In this study, we sampled soil aggregates from two typical alpine ecosystems (alpine meadow and alpine shrubland) during the seasonal FT processes (UFP: unstable freezing period, SFP: stable frozen period, UTP: unstable thawing period and STP: stable thawed period). The phospholipid fatty acid (PLFA) method was used to determine the biomass of living microbes in different aggregate fractions.ResultsThe microbial biomass of 0.25–2 mm and 0.053–0.25 mm aggregates did not change significantly during the seasonal FT process while the microbial biomass of > 2 mm aggregates presented a significant difference between the STP and UTP. Bacterial communities dominated the microbes in aggregates, accounting for over 80% of the total PLFAs. The microbial communities of soil aggregates in the surface layer were more sensitive to the seasonal FT process than those in other soil layers. In the thawing period, Gram positive bacteria (GP) was more dominant. In the freezing period, the ratio of Gram-positive to Gram-negative bacterial PLFAs (GP/GN) was low because the enrichment of plant litter facilitated the formation of organic matter. In the freezing process, pores of 30–80 μm (mesopores) favored the habitation of fungal and actinobacterial communities while total PLFAs and bacterial PLFAs were negatively correlated with mesopores in the thawing process.ConclusionsThe freezing process caused a greater variability in microbial biomass of different aggregate fractions. The thawing process increased the differences in microbial biomass among soil horizons. Mesopores of aggregates supported the habitation of actinobacterial and fungal communities while they were not conducive to bacterial growth. These findings provide a further comprehension of biodiversity and accurate estimation of global carbon cycle.

Structural pathway for nucleation and growth of topologically close-packed phase from parent hexagonal crystal

The solid diffusive phase transformation involving the nucleation and growth of new phase particles is universal and frequently employed but has not yet been fully understood at the atomic level. Here, our first-principles calculations reveal a structural formation pathway of a series of topologically close-packed (TCP) phases within the hexagonally close-packed (hcp) matrix. The results show that the nucleation follows a nonclassical nucleation process, and apart from atomic diffusion, the entire hcp→TCP transformation only involves shuffle-based displacements, with a specific 3-layer unstable hcp-ordering serving as the basic structural transformation unit (BSTU). The thickening of plate-like TCP phases relies on the formation of these unstable hcp-orderings at their coherent TCP/matrix interface to nucleate a ledge, but the ledge lacks the dislocation characteristics which is commonly considered in the conventional view. Additionally, the atomic structure of the critical nucleus for the Mg2Ca and MgZn2 Laves phases was predicted in terms of Classical Nucleation Theory (CNT). The formation of polytypes and off-stoichiometry in TCP precipitates is found to be related to the nonclassical nucleation behavior. These insights contribute to a deeper understanding of solid diffusive phase transformations at the atomic level and provide a foundation for investigating other technologically significant transformations.

Structural Properties of Amorphous Vanadium Pentoxide Under Compression

Structural properties of amorphous vanadium pentoxide (V2O­5) under compression investigated by molecular dynamics simulation. The simulated amorphous V2O5 is composed of basic structural units of type VO5, VO6 at low-pressure and VO6, VO7 at high-pressure. These basic structural units connected by vertex-, edge- and face- shared links to form a structural network. The random distribution of atom and void clusters leading to a high degree of disorder in the V2O5 structure. Under compression, the fraction of average vertex-, edge- and face- shared links increased strongly. The number of VCs and VTs also increases as the large voids are divided into smaller voids. Our results also elucidated the decreasing of cross-section and increasing of length in VTs are main causes for increasing ion conductivity of the amorphous V2O5.

All-organic heterojunctions used for the excellent photocatalytic H2O2 synthesis: The key role of bay-position Cl in PDI

Melem is the basic structural unit of g-C3N4. When directly utilized as a photocatalyst, the separation efficiency of photogenerated electron-hole pairs is low. In this study, we constructed a Melem-based all-organic heterojunction (PICN) by reacting the -NH2 at the terminal of the Melem unit with the anhydride of PDA. The photocatalytic efficiency for H2O2 production of the heterojunction is 4.2 times that of CN. Introducing -Cl at the bay position of PDI further enhances the photocatalytic H2O2 production efficiency by 1.6 times. This enhancement is attributed to the introduction of -Cl, which not only broadens the UV–visible light absorption range of the sample but also improves the interface electron transfer efficiency between Melem and PDICl. Moreover, the introduction of -Cl can also reduce the overpotential of the O2 reduction reaction on the surface of the sample. These novel findings may provide insights for the design of organic heterojunction photocatalysts.

A study on elastic properties of CdO–B2O3 glasses by using ultrasonic pulse-echo technique

Binary xCdO–(100−x)B2O3 glasses (45 mol% ≤ x ≤ 90 mol%) were successfully prepared by the melt-quenching technique. The effect of CdO on structure and elastic properties of the borate network was studied by using ultrasonic pulse-echo technique. Density and molar volume of the glass show an increase with increasing CdO content, whereas, ultrasonic velocities, elastic moduli, micro-hardness and Debye temperature revealed a broad peaks around 75 mol% CdO. These findings were interpreted in terms the types of the basic structural units that composing the glass network. In the composition range 45 mol%≤x≤75 mol%, the addition of CdO, a network modifier, resulted in formation of extra bridging oxygens due to BO3ÆBO4 conversion and, which expands the borate network and stiffening the structure. Beyond x=75 mol%, some of CdO4 structural units and weaker B–O–Cd linkages are formed, besides the creation of NBOs. This opens the structure and decrease the rigidity of the glass. Correlations between elastic properties and compositional parameters are achieved by applying the ring deformation model and Abd El-Moneim’s approach.

Effect of hydrofluoric acid on Guizhou anthracite structure modification and response to enhanced hydrolysis and permeability of coal

ABSTRACT To investigate the structural modification of Guizhou anthracite through the application of hydrofluoric acid and its corresponding response in terms of enhanced gas desorption and permeability, we utilized Guizhou anthracite from a mining area as the focal point of our study. We conducted a series of experiments encompassing X-ray diffraction (XRD), initial gas release velocity measurements, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and nuclear magnetic resonance (NMR). Our investigation identified hydrofluoric acid as the primary acid solution, with an optimal concentration of 6% and a soaking duration of 12 hours. Furthermore, our theoretical analysis unveiled the physicochemical structural evolution of Guizhou anthracite under the influence of hydrofluoric acid and its subsequent response to enhanced gas desorption and permeability within the coal. Regarding chemical structure, hydrofluoric acid effectively dissolves inorganic minerals in anthracite and reduces the condensation of its regular basic structural units. Peak fitting of functional groups on the surfaces of the experimental coal samples revealed that hydrofluoric acid induces side chain reactions in the molecular structure of anthracite, promoting the gradual ordering of the aromatic structure. Simultaneously decreasing the content of cyclically closely bonded and self-associated hydroxyl hydrogen bonds. These alterations in irregular basic structural units can diminish coal’s adsorption capacity for gas, thereby enhancing gas desorption. Regarding the physical structure, treatment with hydrofluoric acid leads to the dissolution of minerals within specific pores and fissures of the coal samples, generating numerous dissolution pores. These pores and fissures expand and interconnect. The surface morphology quantified by fractal dimension has decreased by about 25.8%, indicating that pore and fracture structures often become relatively simple, which is conducive to gas flow and desorption in coal. Furthermore, the effective porosity and total porosity of the coal samples increase, as does the total movable fluid porosity. This enhances pore connectivity within the coal seam. Calculations reveal that after acidification, the permeability of the experimental coal samples increases by approximately 1.37 times, confirming the permeability-enhancing effect of hydrofluoric acid on anthracite.

Superpentagraphene C34: A two-dimensional network structure of C20 fullerene

The well-known smallest icosahedral fullerene C20 is comprising five-membered carbon-rings in all sp2 bonding states. Here we identify by ab initio calculations a new quasi two-dimensional (2D) carbon allotrope in sp2-sp3 bonding states, comprising the unusual five-membered C20 fullerene as the basic structural units, with a 34-atom hexagonal cell in P6/mmm(D6h1) symmetry, and term it as spg-C34 superpentagraphene. Total energy calculations show that it is energetically more stable than the previously reported 2D carbon forms such as pentagraphene and ph-graphene. Its structural stability has been verified by phonon mode analysis and ab initio molecular dynamics simulations. Electronic band calculations reveal that this 2D spg-C34 carbon is a semiconductor with a sizable indirect band gap of 3.31 eV. Moreover, we show that such 2D spg-C34 carbon structure has a larger layer modulus (281 N/m), a larger in-plane stiffness (524 N/m), and a smaller Poisson's ratio (0.069) than the values for graphene. These results establish a new exotic quasi-2D carbon phase and offer insights into its outstanding electronic and mechanical properties of two-dimensional carbon structure.

Graphitization: Microstructural and microtextural transformations of residual char from international coal combustion ash

Natural graphite is a critical raw material and its substitution in some applications will contribute to reduce its supply risk. Coal ash, which is still produced in high amounts, contains a carbonaceous solid residue (char) that may be a substitute of natural graphite. For this purpose, char may be graphitized and used in electrocatalysis reactions, however char properties depend on coal rank, composition and combustion conditions that affect the graphitization process. Research on industrial coal char graphitization is limited, resulting in restricted comprehension of the contributing factors. In order to provide novel insights, industrial residual chars (derived from Poland, Portugal, Romania and South Africa) subjected to high-temperature treatments (carbonization at 1000 °C followed by treatment at 2600 °C under environmental pressure) were examined regarding their microstructural and microtextural transformations. The samples were studied by focussing on its heterogeneity, namely its specific properties and interactions, including elemental composition, optical character, and structure. Furthermore, the evolution of structural order of selected char morphotypes in samples derived from coal of similar rank was assessed using Raman microspectroscopy.The results highlighted a set of aspects that could have influenced the transformations experienced by the different chars during high temperature treatments (graphitization). It was found that Hydrogen might play a role in the graphitization ability of isotropic chars derived from low rank coal. The prior preferential orientation of the Basic Structural Units (BSU) contributes to achieve a higher graphitization degree, but it can be enhanced or hindered by other factors, e.g., hydrogen content. Raman microspectroscopy showed that char morphotypes from the same group underwent different transformations during graphitization, indicating that morphotypes or sections with unfused optical character may be more prone to graphitization. However, the existence of disordered domains and specific microtextures, such as polyhedral pores identified under TEM, likely hindered further graphitization. Further research on this topic is needed.

Path for Room-Temperature Superconductivity in Q-Carbon-Related Materials

We present the atomic structures and nonequilibrium synthesis of a new class of materials, where the basic structural unit is a diamond tetrahedron. When units of one, two, and three tetrahedra are randomly packed, we create distinct phases of amorphous Q-carbon. Four tetrahedra in two adjacent layers lead to crystalline diamond lattice, which has four missing tetrahedra alternately. When these four missing tetrahedra are filled, we create subunit cell of crystalline Q-diamond. Theoretical calculations show that the superconducting transition temperature (Tc) in 50 atomic % B-doped Q-diamond can reach room temperature at ambient pressures. This is consistent with our earlier results using low-loss EELS measurements in 50 atomic % B-doped Q-carbon, which had mostly amorphous QB3 phase mixed with some crystalline Q-diamond phase. These EELS results showed that the Tc for these samples was between 90 K and 300 K. Theoretical calculations of density of states, Eliashberg function, electron–phonon interaction parameter, and root-mean-square and logarithmic average of frequency in crystalline Q-diamond show Tc in the range of 268 K to 300 K, which is in complete agreement with our EELS results in QB3.

Poly(ADP-ribosyl)ation enhances nucleosome dynamics and organizes DNA damage repair components within biomolecular condensates

Nucleosomes, the basic structural units of chromatin, hinder recruitment and activity of various DNA repair proteins, necessitating modifications that enhance DNA accessibility. Poly(ADP-ribosyl)ation (PARylation) of proteins near damage sites is an essential initiation step in several DNA-repair pathways; however, its effects on nucleosome structural dynamics and organization are unclear. Using NMR, cryoelectron microscopy (cryo-EM), and biochemical assays, we show that PARylation enhances motions of the histone H3 tail and DNA, leaving the configuration of the core intact while also stimulating nuclease digestion and ligation of nicked nucleosomal DNA by LIG3. PARylation disrupted interactions between nucleosomes, preventing self-association. Addition of LIG3 and XRCC1 to PARylated nucleosomes generated condensates that selectively partition DNA repair-associated proteins in a PAR- and phosphorylation-dependent manner invitro. Our results establish that PARylation influences nucleosomes across different length scales, extending from the atom-level motions of histone tails to the mesoscale formation of condensates with selective compositions.

Progress in the Metabolic Engineering of Yarrowia lipolytica for the Synthesis of Terpenes.

Terpenes are natural secondary metabolites with isoprene as the basic structural unit; they are widely found in nature and have potential applications as advanced fuels, pharmaceutical ingredients, and agricultural chemicals. However, traditional methods are inefficient for obtaining terpenes because of complex processes, low yields, and environmental unfriendliness. The unconventional oleaginous yeast Yarrowia lipolytica, with a clear genetic background and complete gene editing tools, has attracted increasing attention for terpenoid synthesis. Here, we review the synthetic biology tools for Y. lipolytica, including promoters, terminators, selection markers, and autonomously replicating sequences. The progress and emerging trends in the metabolic engineering of Y. lipolytica for terpenoid synthesis are further summarized. Finally, potential future research directions are envisioned.