Basal Stalk Rot Research Articles

Candidate gene association mapping of Sclerotinia stalk rot resistance in sunflower (Helianthus annuus L.) uncovers the importance of COI1 homologs

Functional markers for Sclerotinia basal stalk rot resistance in sunflower were obtained using gene-level information from the model species Arabidopsis thaliana. Sclerotinia stalk rot, caused by Sclerotinia sclerotiorum, is one of the most destructive diseases of sunflower (Helianthus annuus L.) worldwide. Markers for genes controlling resistance to S. sclerotiorum will enable efficient marker-assisted selection (MAS). We sequenced eight candidate genes homologous to Arabidopsis thaliana defense genes known to be associated with Sclerotinia disease resistance in a sunflower association mapping population evaluated for Sclerotinia stalk rot resistance. The total candidate gene sequence regions covered a concatenated length of 3,791 bp per individual. A total of 187 polymorphic sites were detected for all candidate gene sequences, 149 of which were single nucleotide polymorphisms (SNPs) and 38 were insertions/deletions. Eight SNPs in the coding regions led to changes in amino acid codons. Linkage disequilibrium decay throughout the candidate gene regions declined on average to an r (2) = 0.2 for genetic intervals of 120 bp, but extended up to 350 bp with r (2) = 0.1. A general linear model with modification to account for population structure was found the best fitting model for this population and was used for association mapping. Both HaCOI1-1 and HaCOI1-2 were found to be strongly associated with Sclerotinia stalk rot resistance and explained 7.4 % of phenotypic variation in this population. These SNP markers associated with Sclerotinia stalk rot resistance can potentially be applied to the selection of favorable genotypes, which will significantly improve the efficiency of MAS during the development of stalk rot resistant cultivars.

First Report of Sclerotinia sclerotiorum Infection on Cuphea.

Species of the genus Cuphea (family Lythraceae) are being developed as potential domestic sources of medium length fatty acids (lauric and capric) for use in industrial lubricants and detergents. During September 2004, patches of dead plants were observed in test plots of Cuphea sp. cv. PSR-23 (1) (Cuphea viscosissima Jacq. × C. lanceolata W.T. Aiton) near Morris, MN and Prosper, ND, approximately 200 km apart. Seed yield in the diseased Morris field was 78 kg/ha compared with 516 kg/ha in nearby, nonaffected fields of the same variety, for an 85% yield reduction. Stems were split open to reveal long, cylindrical sclerotia as much as 8 mm long. Isolations from diseased stem tissue and sclerotia were identified as Sclerotinia sclerotiorum (Lib.) de Bary and produced typical sized sclerotia (4 to 6 mm in diameter) after 7 days growth on potato dextrose agar (PDA). Cuphea PSR-23 plants were grown in the greenhouse in individual pots for 5 weeks and then inoculated. Three inoculation methods were used. For the first method, ascospores of a sunflower isolate of S. sclerotiorum were sprayed onto blooming flowers and foliage at a rate of 5,000 spores per ml. The inoculated plants were kept in a dark, 18°C mist chamber for 48 h and then returned to a greenhouse maintained at 24/20°C, day/night temperatures. All 20 inoculated plants were visibly colonized by Sclerotinia sp. after 3 days, and all plants were dead by 7 days. The second inoculation used the petiole inoculation technique employed by canola researchers (2). The blade from the third leaf was excised and a micropipette tip containing an agar disk of mycelia of the Cuphea isolate was placed over the cut end of the petiole. Five days after inoculation, all 30 inoculated plants were dead, while none of the 10 control plants (using sterile agar disks on the cut petiole) were affected. Isolations were made from diseased plants inoculated by all methods, and S. sclerotiorum colonies were observed on PDA medium with typical sclerotia from 4 to 6 mm in diameter. The third inoculation method tested root infection. S. sclerotiorum was grown on autoclaved proso millet (Panicum miliaceum L.) seed for 7 days, and 5 g of colonized millet seed was placed in a hole 6 cm from the base of a Cuphea plant, with one plant per 3.7 liter pot. Sunflower (Helianthus annuus L.; oilseed hybrid Cargill 270) plants served as inoculated controls. None of the 20 Cuphea plants were infected via soil inoculations compared with 70% of 30 sunflower plants that developed basal stalk rot and wilt within 2 weeks after inoculation. To our knowledge, this is the first report of S. sclerotiorum infection on Cuphea sp., and is believed to be the first report of infection on any genus within the Lythraceae (loosestrife family). With over 100 annual and perennial species in the genus Cuphea, the possibility of Sclerotinia spp. resistance needs to be investigated to further develop this potential oilseed crop.

Pythiogeton zizaniae, a new species causing basal stalk rot of water bamboo in Taiwan

A new species, Pythiogeton zizaniae, was isolated from diseased water bamboo (Zizania latifolia) in central Taiwan. The organism formed a colony with scanty mycelia and mycelial aggregates on rye-water bamboo medium. Special treatments were required for production of sporangia which were terminal, noncaducous and mostly ovoid. Chlamydospores were absent. The fungus was homothallic. Oogonia produced on V-8 water bamboo medium in water were mostly globose to subglobose and each was attached with a club-shaped, monoclinous antheridium by the base of the oogonium stalk. Oospores were plerotic and globose to subglobose. Py. zizaniae caused death of water bamboo suckers but did not infect seedlings of corn, rice, wheat, sorghum, cucumber, tomato, soybean or water spinach. It also did not affect cucumber and tomato fruit, carrot roots or potato tubers.

Pythiogeton zizaniae, a new species causing basal stalk rot of water bamboo in Taiwan

A new species, Pythiogeton zizaniae, was isolated from diseased water bamboo (Zizania latifolia) in central Taiwan. The organism formed a colony with scanty mycelia and mycelial aggregates on rye-water bamboo medium. Special treatments were required for production of sporangia which were terminal, noncaducous and mostly ovoid. Chlamydospores were absent. The fungus was homothallic. Oogonia produced on V-8 water bamboo medium in water were mostly globose to subglobose and each was attached with a club-shaped, monoclinous antheridium by the base of the oogonium stalk. Oospores were plerotic and globose to subglobose. Py. zizaniae caused death of water bamboo suckers but did not infect seedlings of corn, rice, wheat, sorghum, cucumber, tomato, soybean or water spinach. It also did not affect cucumber and tomato fruit, carrot roots or potato tubers.

Crop sequence effects of 10 crops in the northern Great Plains

Dynamic cropping systems, which involve a long-term strategy of annual crop sequencing, require detailed information on management components known to influence crop performance. Considering that proper sequencing of crops is an important component for successful dynamic cropping systems, a research project was undertaken to determine the advantages and/or disadvantages of previous crop and crop residues for numerous crop sequences. A multi-disciplinary team of scientists evaluated crop sequence effects of 10 crops (barley, canola, crambe, dry bean, dry pea, flax, safflower, soybean, spring wheat, and sunflower) on seed yield, soil coverage by residue, soil water use, surface soil properties, and plant diseases in central North Dakota. Two years were required to establish a crop by crop residue matrix (crop matrix). During the second year (site 1, 1999; site 2, 2000) 10 crops were evaluated with a crop matrix. During the third and fourth year spring wheat (site 1, 2000; site 2, 2001) and sunflower (site 1, 2001; site 2, 2002), respectively, were seeded over the crop matrix. The seed yield of four crops (crambe, flax, safflower, and soybean) of the 10 crops evaluated in the crop matrix was influenced by the preceding crop at site 1 in 1999 an above average moisture year. The seed yield of eight crops (canola, crambe, dry bean, flax, safflower, soybean, spring wheat, and barley) was influenced by the preceding crop at site 2 in 2000 a more average precipitation year. Some of the lowest seed yields were obtained when a crop was seeded on its own residue. A synthesis of seed yield data from a given year provided overall values for positive and negative effects of crops and crop residue on subsequent crops. In general, the three legume crops had positive effects in contrast to non-leguminous crops, which usually had negative effects. However, sunflower was an exception among the non-leguminous crops; at site 2 in 2000, sunflower was positive for subsequent crops compared with canola or crambe, which had negative effects. Crop sequences composed of small cereal grains had the highest soil coverage by residue while sequences of two dicotyledonous species had considerably lower coverage. Soil water use among crops varied, ranging from sunflower with the numerically highest soil water use to dry pea with the least. Significant changes in surface soil properties due to crops were generally not detected in this short-term project. Given the variation in Sclerotinia disease incidence for canola, crambe, safflower, and sunflower within the crop matrix, it was difficult to detect significant differences based on the previous crop. Differences were evident two years later when the highest incidence of Sclerotinia basal stalk rot for sunflower was detected in plots where crambe was grown two years earlier. During the third year, when spring wheat was seeded over a crop matrix, spring wheat yields increased following 23 and 19 crop sequence treatments out of a possible 100 at site 1 and site 2, respectively, compared to the continuous wheat treatment. All crop sequence treatments that yielded better than the continuous wheat treatment were comprised of mostly non-cereal crops, demonstrating the positive impact of crop diversity on cereal crop production. The severity of leaf spot diseases on spring wheat were affected by crop sequence and fungal spore production was greatest on the continuous wheat treatment. Even though decreases in leaf spot disease severity and modest yield increases were obtained with some crop sequence treatments, significant yield increases due to reduced leaf spot disease severity were not obtained under our conditions.

Pythiogeton zeae sp. nov. Causing Root and Basal Stalk Rot of Corn in Korea

In South Korea, a pythiaceous fungus was consistently isolated from the diseased tissues of field corn plants (Zea mays) showing severe rot of the roots and basal stalks. Based on the morphology, i...

Pythiogeton zeae sp. nov. causing root and basal stalk rot of corn in Korea

In South Korea, a pythiaceous fungus was consistently isolated from the diseased tissues of field corn plants (Zea mays) showing severe rot of the roots and basal stalks. Based on the morphology, it was described as Pythiogeton zeae sp. nov., and a taxonomic key was constructed to differentiate it from the other known species of Pythiogeton. It is characterized by small to extremely large, ovoid, cylindrical, bursiform, ventricose, bilobulate, trilobulate to irregular saccate sporangia, large globose to ellipsoidal oogonium, plerotic oospore with thick wall and mostly single, diclinous or monoclinous antheridium. Its pathogenicity to corn plants was confirmed by artificial inoculation and successful re-isolation. This is the first documented report of a plant-pathogenic species of Pythiogeton, a genus known to be saprophytic usually on plant debris in freshwater.

Association of Phoma terrestris, Pythium irregulare, and Fusarium acuminatum in Causing Red Root Rot of Corn.

Greenhouse and field tests were conducted in 1992 and 1993 to determine the causal pathogen(s) of red root rot (RRR) of corn. Corn hybrids Dekalb DK 522, DK 572, DK 677, and DK 582 were utilized. Phoma terrestris, Pythium irregulare, and Fusarium acuminatum were used alone or in combination to infest potting mix in greenhouse tests or soil in field tests. Results indicated that P. terrestris is the primary pathogen in the RRR complex of corn in Delaware. When P. terrestris and Pythium irregulare were associated, the disease progressed faster and was more severe, resulting in significantly higher (P ≤ 0.01) root rot, basal stalk rot, and wilt. F. acuminatum played a minor role in causing the disease, with little additional symptom development when combined with P. terrestris or Pythium irregulare.

Sclerotinia and Phomopsis — two devastating sunflower pathogens

Cultivated sunflower (Helianthus annuus L.) is prone to many diseases caused by fungi, bacteria, and viruses, of which the most detrimental are those caused by fungi. Two of the most important fungal pathogens are Sclerotinia and Phomopsis. Within the genus Sclerotinia, both S. minor and S. sclerotiorum cause a basal stalk rot and subsequent wilt of sunflower, with S. sclerotiorum more widely distributed around the world. Sclerotinia sclerotiorum also incites a head rot and mid-stem rot of sunflower. Since Sclerotinia spp. have a very wide host range and can persist in soil as dormant sclerotia for up to 10 years, the pathogens have been extremely difficult to control with either generic resistance or fungicides. The most effective control is the use of long rotations, tolerant hybrids, and sclerotium-free seed. Gray stem spot is a relatively new disease of sunflower first observed in the early 1980s in Yugoslavia and now considered the most devastating sunflower disease in eastern Europe. Symptoms include large stem cankers and subsequent wilt. Phomopsis helianthi has been identified as one incitant of gray stem spot, but another Phomopsis species may also be involved, both in Europe and in the United States. Foliar fungicides can control gray stem spot, but the development of resistant hybrids has been much more cost-effective.

Indicators of resistance of sunflower plant to basal stalk rot (Sclerotinia sclerotiorum): Symptomatological, biochemical, anatomical, and morphological characters of the host

The object of this work was a study of the relationship between the field reactions of different sunflower genotypes to basal stalk rot (in terms of severe (dead plants) and incipient wilting, and lesion length) and some biochemical (phenol concentration), morphological (plant height, and stem and flower-bud diameters) and anatomical (xylem and cortical indexes) characters of the host. Plants from 8 inbred lines at closed flower-bud stage were artificially inoculated with mycelium at the base of the stem. The percentage of dead plants for each inbred line and the lesion length and wilting range for individual plants after 7 days were recorded. A positive and highly significant correlation coefficient between the percentage of dead plants and lesion length was found for the three years of the study (r=0.83; P<0.01). A highly significant association between lesion length and wilting range for individual plants was always found (P=0.00). Postinfectional phenol content exhibited a strong negative correlation with lesion length and the percentage of dead plants in all the experiments (P=0.05). Association between postinfectional phenol content and wilting range for individual plants was significant for all the years studied (P<0.05). No correlation between phenol levels in healthy plants of the different sunflower genotypes and their susceptibility was found. Morphological characters positively correlated with lesion length but only plant height exhibited significant values for the three years. Associations between wilting range and morphological characters for individual plants were significant for one of the two years analyzed (P<0.05). Xylem index showed a negative correlation with lesion length which was significant one of the two years studied. The lesion length measure seems to be a simple and direct method for resistance screening before the flowering period. Although strong relations with postinfectional phenol levels were found, their determinations would be too much time consuming and not completely reliable. The relationships between other characters measured and disease resistance would indicate that physiological mechanisms could be related to resistance.

Effects of acidic fog on productivity of celery and lettuce and impact on incidence and severity of diseases

SummaryThe effects of acidic fog on productivity of celery and lettuce were examined using a portable fogging apparatus to expose field plots to simulated fog episodes. Acidic solutions were formulated to simulate fog reported for southern California. Celery (Apium graveolens var. dulec cv. Bishop) and lettuce (Lactuca sativa cv. Mesa 659) were exposed in separate experiments once or twice each week during crop growth and maturation to 1 ‐ to 2‐h of fog at specific acidity levels between pH 1.6 and 3.8. Necrosis occurred as small, irregularly shaped white (celery) or brown (lettuce) lesions on the most exposed portions of the plants. Fog necrosis sufficient to reduce marketability of celery was evident after single exposures at pH 2.4. Although injury occurred on wrapper leaves of lettuce at acidity levels as high as pH 3.4, marketability was reduced from foliar necrosis only after exposure to acidity levels of pH 2.6 or lower. Repeated exposure of lettuce to acidity below pH 2.0 was necessary to reduce yield. The field experiments demonstrated that acidic fog influenced natural incidence of disease. At low pH fog exposure, celery had increased occurrence of basal stalk rot and lettuce exhibited increased bacterial soft rot. Indirect effects of acidic fog on host‐pathogen relationships and subsequent crop marketability may be more important than the direct effects of acidic fog exposure on plant tissue.

PRELIMINARY STUDIES OF ROOT AND BASAL STALK ROT OF MATURING CORN IN ONTARIO

In Ontario, basal stalk rot of corn occurs in the autumn and is of major importance, whereas bacterial stalk rot is of no economic significance. The latter disease, which results from stomatal penetration by Erwinia dissolvens, may occur on a few susceptible corn plants in early July during extremely hot humid weather, although ordinarily temperatures are sufficiently low in the province to limit its development. Common basal stalk rot of corn is usually initiated by the spread of organisms from diseased corn roots up into the stalks; rotting may begin also in tunnels produced by corn borers. Diplodia zeae and Gibberella zeae, the two pathogens that are responsible for most of the stalk rotting in the central part of the American corn belt, were of no practical importance during the last two years. Bacteria, Pythium arrhenomanes, and Fusarium moniliforme were commonly found in the necrotic part of the corn stalk. Diseased plants begin to appear in mid-August and daily become more numerous until the first killing frost occurs or the plants become senescent. The leaves suddenly droop and wither, and are dead within four or five days after the first symptoms appear. By this time, the basal part of the stalk is necrotic and at least some of the roots are decayed. Pith tissues are destroyed and the stalk frequently breaks over near the ground level. All stalk rot pathogens finally produce the same syndrome. P. arrhenomanes causes a root necrosis at first which later spreads up into the stalk and, in Ontario, is of major importance in the corn stalk rot disease. Pratylenchus pratensis has been found in corn roots but its importance is unknown.