Objectives: The aim of this study is to examine the factors influencing e-commerce adoption (ECA) among SMEs and evaluate its impact on their business performance. The research employs a mixed-methods approach, combining quantitative surveys and qualitative interviews with small and medium enterprises (SMEs) owners and managers across diverse industries and geographical locations.Methods: The analysis involved collecting and scrutinizing data from various organizations to provide a quantitative understanding of the relationship between organizational culture and ECA perceptions among top management. The factorability of the collected data was tested using the Bartlett’s test of sphericity and the Kaiser-Meyer-Olkin (KMO) test, which are commonly used to assess the appropriateness of data for factor analysis.Findings: This work underscores the importance of acknowledging the role of culture in ECA and emphasizes the significance of further research and strategies for fostering a culture that promotes and supports e-commerce within enterprises. Finally, barriers of ECA and Influencing and user satisfaction factors are identified and the conceptual framework of factors influencing ecommerce adoption in SMEs (Service sector) of ASSAM is developed.Novelty: Applying these tests specifically to the context of ECA in SMEs is a novel application.