Accurately assessing the changes in soil organic carbon storage (SOCS) before and after the Grain for Green Project (GFG) in the Loess Plateau (LP) and exploring the relationship between its spatial and temporal distribution and the influencing factors were important references for the development of regional recycling as well as the formulation of ecological protection policies. Based on the data of climate, human activities, and SOCD in the surface (0-20 cm) and deep (0-100 cm) soil before and after GFG in the LP from 2001 to 2020, we investigated the changes in SOCD at different spatial and temporal scales by using the methods of trend analysis, the kriging method, and variance partitioning analysis. The results showed that: ① Before and after the GFG, the surface SOCS of the whole region increased by 8 338.7×104 t; the deep SOCS increased by 1 160.02×104 t. ② In each bioclimatic subregion, the whole-region average SOCD of Ⅰ (Semi-Humid Forest Region), Ⅱ (Semi-Humid Semi-Arid Forest and Grassland Region), and Ⅲ (Semi-Arid Typical Grassland Region) showed a significant increasing trend, with a decreasing trend in Ⅳ (arid semi-arid desert grassland area) and Ⅴ (arid desert area). ③ The average surface SOCS increase in different ecosystems was ranked as follows: cropland > grassland > woodland > shrubs > bare land and sparse vegetation. The deep soil increase was ranked as follows: grassland > cropland > woodland > shrubs > bare land and sparse vegetation. ④ Climate factors were the most important driving factors for changes in SOCD; the annual average temperature and precipitation were significantly positively correlated with changes in SOCD. The results of the study could provide data support for regional ecological management and land use policy formulation to promote high quality development of the ecological environment in the LP.
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