BARI Mung-6 Research Articles

Effect of Manures with Fertilizers on Yield and Yield Traits of Mungbean (Vigna radiate L.)

An experiment was carried out at the Agronomy Field of Sher-e-Bangla Agricultural University, Dhaka during the period from March to June 2015 (Kharif-I season) to find out the effect of different manures along with inorganic fertilizers on yield and yield traits of mungbean varieties. The experiment consisted of two factors: factor A: five levels of manures along with inorganic fertilizers [ T0 = Control (no fertilizer or manure), T1 = Recommended dose of fertilizer (R) (45 kg urea ha-1 + 100 kg TSP ha-1 + 58 kg MoP ha-1), T2 = R + cowdung (3 t ha-1), T3 = R + poultry manure (2 t ha-1), T4 = R + vermicompost (2.5 t ha-1)] and factor B: two mungbean varieties; (V1 = BARI Mung 5 and V2 = BARI Mung 6). The experiment was laid out in split- plot design with three replications. The effect of manures along with recommended inorganic fertilizers on yield and yield traits were found significant. The maximum grain yield (1.53 t ha-1) and maximum stover yield (1.88 t ha-1) was recorded from T4 treatment. Mungbean variety had also significant influence on yield and yield traits. The maximum seed yield (1.58 t ha-1) was obtained from var. BARI Mung 6) but Maximum stover yield (1.91 t ha-1) was obtained from var. BARI Mung 5). The combined effects of manures along with recommended inorganic fertilizers and variety were found statically significant on yield and yield traits. The maximum seed yield (2.01 t ha-1) and maximum stover yield (2.61 t ha-1) was recorded from T4V2. There were positive correlations of seed yield with different yield components. It was comparatively stronger correlations in BARI Mung 5 and weaker in BARI Mung 6. So, var. BARI Mung 6 performed the best result with the application of vermi-compost @ 2.5 t ha-1 with recommended dose of fertilizer.Bangladesh Agron. J. 2017, 20(2): 115-122

Physiological Traits and Yield Performance of Mungbean (Vigna radiata (L) Wilczek) as Influenced by Light Intensities

An experiment was carried out at the Crop Physiology and Ecology Research Field, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur during the period from March to June 2016 to evaluate some physiological traits and yield performance of mungbean variety under different light intensities and to find out potential mungbean variety as intercrop. The experiment was laid out in a split -plot design with three replications. Three light intensities (L100 - full sunlight, L75- 75 % of full sunlight and L50- 50% of full sunlight) were assigned in the main plots and four varieties (BARl Mung-6, Binamoong8, Binamoong5 and BU Mug-4) in the subplots. White and red colour Mosquito nets were used for maintaining 75 and 50 percent of full sunlight. Light percentages of mosquito nets were measured by light meter (21YE35). Mosquito nets of different colors and pore size were used for maintaining 75 and 50 percent of full sunlight. The seed yield of BARI Mung-6 and Binamoong8 performing well in under partial shade condition but the grain yield of Binamoong5 and BU Mug-4 was reduced drastically under partial shade condition. Greater proline accumulation in leaf, higher leaf chlorophyll content, higher pods plant-1, higher seeds pod-1, greater seed size and better seed yield plant-1 under partial shade condition were contributed to better tolerance of BARI Mung-6 and Binamoong8 under low light stress.Bangladesh Agron. J. 2017, 20(2): 67-74

Leaf Characteristics and Yield Performance of Mungbean (<i>Vigna radiata</i> L.) Varieties under Different Levels of Shading

An experiment was carried out to evaluate the leaf characteristics and yield performances of mungbean (Vigna radiata L.) under different light levels at the Crop Physiology and Ecology Research Field of Hajee Mohammad Danesh Science and Technology University, Dinajpur during March to June 2016. The experiment was laid out in a split plot design with three replications. Three light levels (L100 - 100 % light intensity, L75- 75 % light intensity and L50- 50% light intensity) were assigned in the main plots and four varieties (BARl Mung-6, BINA Mung-8, BINA Mung-5 and BU Mug-4) were assigned in subplots. Mosquito nets of different pore size were used for maintaining 75 and 50 percent light intensity. Leaf area was increased due to reduced light levels in all mugbean varieties but the increment was significant in BINA Mung-5 and BINA Mung-8 only at 75% light intensity at 40 days after sowing and only in BARI Mung-6 with L50 and BU Mug-4 with L75 and L50at 50 days after sowing. Due to reduced light levels, leaf dry weight was affected more in BINA Mung-5 and BU Mug- 4 than BARI Mung-6 and BINA Mung-8. Leaf thickness was reduced under shade in all the mungbean varieties, except in BU Mug-4 at 75% light intensity, and the reduction in leaf thickness was mainly due to the reduction in thickness of spongy layer. The palisade layer thickness was influenced insignificantly but spongy layer thickness was increased in BINA Mung-5 at 100% light intensity. The grain yields (t ha-1) of BARI Mung-6 and BINA Mung-8 remained stable under partial shade condition but the grain yield of BINA Mung-5 and BU Mug-4 was reduced drastically under partial shade condition. Higher leaf dry weight, number of pods plant-1, seeds pod-1, and heavier grains in BARI Mung-6 and BINA Mung-8 contributed to the higher grain yield plant-1 under partial shade condition than in BINA Mung-5 and BU Mug-4.The Agriculturists 2017; 15(2) 40-51

Feasibility of Introducing Rice Based Four Crops Pattern in Rangpur Region of Bangladesh

An experiment was conducted at Bangladesh Rice Research Institute (BRRI) Farm at Rangpur during July 2014 - June 2016 to introduce a 4-crops pattern with the aim of increasing cropping intensity, productivity, income and soil fertility. The experimental design was RCB with 3 replications. Four cropping patterns were tested which were: CP1 = T. Aman (BRRI dhan62)-Potato (Cardinal)-Mungbean (BARI Mung-6)-T. Aus (BRRI dhan48), CP2 = T. Aman (BRRI dhan62)-Mustard (BARI Sorisha-14)-Mungbean (BARI Mung-6)-T. Aus (BRRI dhan48), Farmers’ improved practice (FIP) = T. Aman (BR11)-Potato (Cardinal)-Maize (NK 40) and Farmers’ general practice (FP) = T. Aman (BR11)-Boro (BRRI dhan28)- Fallow. In T. Aman 2014, BRRI dhan62 produced 3.87 and 3.85 t ha-1 grain yields under CP1 & CP2 (4 crops systems), respectively; BR11 gave grain yield of 4.62 and 4.36 t ha-1 in FIP and FP (3 and 2 crop system), respectively. In T. Aman 2015, BRRI dhan62 produced grain yield of 4.23 and 4.19 t ha-1 in CP1 and CP2, and BR11 gave grain yield of 5.95 and 5.92 t ha-1 in FIP and FP, respectively. After T. Aman, potato yield was 24.66 t ha-1 in CP1 and 24.17 t ha-1 in FIP during 2014-15. In CP2, 2nd crop mustard yield was 1.16 t ha-1 and3rd crop mungbean yield was only 0.98 t ha-1.In CP1, 3rd crop mungbean yield was only 0.83 t ha-1. After potato in FIP, maize yield was 8.38 t ha-1. After T. Aman (BR11) in FP, grain yield of BRRI dhan28 was 5.42 t ha-1. The yield of BRRI dhan48 in CP1 and CP2 (as 4th crop) was 4.5 t ha-1. Similar results of different crops were obtained in 2015-16. The rice equivalent yield (REY) was found to be 30.05 & 35.95 t ha-1yr-1 in CP1 during 1st and 2nd yearfollowed by FIP (23.92 & 29.82 t ha-1yr-1, respectively). The REY was higher in 2nd year compared to 1st year. In 2014-15 the highest net return of Tk.2,42,560 ha-1 was observed in CP1 followed by FIP(Tk.1,58,380 ha-1); in 2015-16, the highest net return was in CP1 (Tk.2,16,960 ha-1) followed by FIP(Tk.1,24,620 ha-1). Potato based cropping pattern was thus, the most suitable and profitable pattern in this area. T. Aman (BRRI dhan62)-Potato (Cardinal)-Mungbean (BARI Mung-6)-T. Aus (BRRI dhan48) may, therefore, be the most suitable and profitable cropping pattern in medium high lands of Rangpur region. The Agriculturists 2017; 15(1) 116-126

Effect of nitrogen on growth and yield on mungbean in low nutrient soil

A pot experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during kharif II season of 2011 to investigate the growth, dry matter production and yield of mungbean genotypes under nutrient stress soil. Ten mungbean genotypes viz., IPSA-12, GK-27, IPSA-3, IPSA-5, ACC12890053, GK-63, ACC12890055, BARI Mung-6, BUmug- 4 and Bina moog- 5 and six nitrogen fertilizer levels viz., 0, 20, 40, 60, 80 and 100 kg N ha-1 were included as experimental treatments. Results revealed that increasing nitrogen level in nutrient stress soil increased growth and dry matter production up to 60 kg N ha-1 irrespective of genotype and thereafter decreased. Among the mungbean genotype IPSA 12 showed maximum leaf area, dry matter production and seed yield (14.22 g plant-1) in nutrient stress soil. The lowest seed yield (7.33 g plant -1) was recorded in ACC12890053 under control condition.Bangladesh J. Agril. Res. 42(1): 77-85, March 2017

Effect of variety and seed rate of mungbean on weed suppression and yield under strip tillage system

Mungbean can successfully be grown under strip tillage system if appropriate seeding rate can determine and adjust with machine. Due to high weed pressure in strip tillage system, selection of such mungbean varieties that have some potentialities to suppress weeds need to find out and moreover, determining optimum seed rate for different varieties could help to achieve best yield. Therefore, a study was conducted at Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh during March-June, 2013 to study the effect of variety and seed rate on weed suppression and yield performance of mungbean under strip tillage system. The study comprised three mungbean varieties (BARI mung-6, BINA mung-5 and BINA mung-8) and five seed rates (20, 25, 30, 35 and 40 kg ha-1). The experiment was laid out in split-plot design with three replications. The study exposed that variety and seed rate had significant effect on plant population of mungbean, weed dry matter, number of pods plant-1, pod length, number of seeds pod-1, seed yield and stover yield of mungbean. The study results clearly found that weed population significantly reduced with increasing seeding rate of mungbean. Seeding of mungbean at 40 kg ha-1 and 35 kg ha-1 could successfully suppress weed under strip tillage system. Moreover, BINA mung-8 and BINA mung-5 had the more potentiality to suppress weeds compare to BARI mung-6. However, BINA mung-5 was found the superior among the three tested varieties that produced the highest seed yield from 35 kg ha-1 seed rate. In case of BARI mung-6 and BINA mung-8, maximum seed yield was also obtained from 35 kg ha-1 seed rate. Therefore, the study concluded that seeding of any of the three mungbean varieties at 35 kg ha-1 rate may help to obtain the satisfactory yield but the study suggest cultivation of BINA mung-5 at 35 kg ha-1 seeding rate under strip tillage system that will give not only the optimum seed yield but also provide good weed suppression.J. Bangladesh Agril. Univ. 14(1): 49-56, June 2016

Polyamines-induced aluminum tolerance in mung bean: A study on antioxidant defense and methylglyoxal detoxification systems.

We investigated the roles of exogenously applied Spd (0.3 mM spermidine) in alleviating Al (AlCl3, 0.5 mM, 48 and 72 h)- induced injury in mung bean seedlings (Vigna radiata L. cv. BARI Mung-2). Aluminum toxicity induced oxidative damage overproducing reactive oxygen species (ROS; H2O2 and O2•-), increasing lipoxygenase activity and membrane lipid peroxidation. The toxic compound methylglyoxal (MG) also overproduced under Al stress. In order to circumvent Al-induced oxidative stress, enzymatic and non-enzymatic antioxidant defense were activated by the application of exogenous Spd. Exogenous Spd increased ascorbate (AsA) and glutathione (GSH) content, AsA/dehydroascorbate (DHA) ratio, GSH/ glutathione disulfide (GSSG) ratio, activity of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR) and catalase (CAT) which reduced ROS production and oxidative stress under Al stress. Spd-induced improvement of GSH pool and Gly II activity alleviated injurious effects of MG. Exogenous Spd positively modulated the endogenous PAs level. Regulating the osmoprotectant molecule (proline), Spd improved plant water status under Al stress. Exogenous Spd was potent to prevent breakdown of Al-induced photosynthetic pigment and to improve growth performances under Al stress. The mechanism by which Spd enhances antioxidant and glyoxalase components might be studied extensively. Spermidine-induced protection of photosynthetic pigment from damages and growth enhancement were remarkable and recommended for further detailed study to understand the mechanism.

Effect of Variety and Seed Rate on Yield Performance of Mungbean Under Strip Tillage System

A study was conducted at Bangladesh Agricultural University, Mymensingh during March-June, 2013 to evaluate the performance of mungbean varieties and to determine the optimum seed rate under strip tillage system. Three mungbean varieties (BARI Mung-6, Binamung-5 and Binamung-8) were tested against five seeding rates (20, 25, 30, 35 and 40 kg ha-1). The experiment was laid out in splitplot design with three replications. The study revealed that variety and seed rate had significant effect on plant population, pods plant-1, pod length, seeds pod-1, seed yield and stover yield of mungbean. The highest seed yield was obtained from Bina mung-5 at 35 kg seed ha-1 while the highest stover yield was obtained from 40 kg seed ha-1. For BARI Mung-6 and Binamung-8, 35 kg seed ha-1 also provided higher seed yield than other seeding rates. Nevertheless, the lowest seed and stover yields were obtained from Bina mung-8 with 20 kg seed ha-1. Therefore, the study concluded that machine seeding of mungbean at 35 kg seed ha-1 ensure optimum seed yield for tested varieties under strip tillage system and cultivation of Bina mung- 5 at 35 kg seed ha-1 can produce the maximum seed yield under this system.Bangladesh Agron. J. 2016, 19(1): 81-86

Chitosan Suppresses Antioxidant Enzyme Activities for Mitigating Salt Stress in Mungbean Varieties

An experiment was conducted to evaluate the effect of chitosan on the antioxidant enzyme activity and protein content in four Mungbean varieties (BARI Mung3, BARI Mung6, BINA Mung5 & BINA Mung8) under salinity. Each pot having eight kilograms of soil was ready to grow three plants of each variety. The experiment was comprised with four different conditions in triplicates viz. control, saline (40mM Nacl, 25DAS), saline+chitosan (25ppm chitosan, 30DAS on saline condition) and chitosan (25ppm chitosan on control condition). Seed collections was done at 60 DAS followed by data analysis. Protein content (using H2SO4, CuSO4, K2SO4 & selenium in Kjeldahl method), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) (using potassium phosphate buffer, EDTA, H2O2, ascorbate, guaicol) were measured accordingly. Salinity enhanced antioxidant enzyme (CAT (maximum 2.47 mM/ml in BARI Mung3), POD (maximum 0.406 mM/ml in BARI Mung6) and APX (maximum 11.99mM/ml in BINA Mung8)) activities compare to control groups in all four varieties. On the other hand, the significant reduction in protein content during salt stress was drastically increased with chitosan application. However, chitosan played an outstanding stimulating role to reduce antioxidant enzyme activities by scavenging ROS (Reactive Oxygen Species). Therefore, it is suggested that chitosan could be an effective biostimulator to avoid the salinity stress by scavenging ROS.

Physiological and biochemical mechanisms of spermine-induced cadmium stress tolerance in mung bean (Vigna radiata L.) seedlings.

The role of exogenous spermine (0.25 mM Spm, a type of polyamine (PA) in reducing Cd uptake and alleviating Cd toxicity (containing 1 and 1.5mM CdCl2 in the growing media) effects was studied in the mung bean (Vigna radiata L. cv. BARI Mung-2) plant. Exogenously applied Spm reduced Cd content, accumulation, and translocation in different plant parts. Increasing phytochelatin content, exogenous Spm reduced Cd accumulation and translocation. Spm application reduced the Cd-induced oxidative damage which was reflected from the reduction of H2O2 content, O2•- generation rate, lipoxygenase (LOX) activity, and lipid peroxidation level and also reflected from the reduction of spots of H2O2 and O2•- from mung bean leaves (compared to control treatment). Spm pretreatment increased non-enzymatic antioxidant contents (ascorbate, AsA, and glutathione, GSH) and activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) which reduced oxidative stress. The cytotoxicity of methylglyoxal (MG) is also reduced by exogenous Spm because it enhanced glyoxalase system enzymes and components. Through osmoregulation, Spm maintained a better water status of Cd-affected mung bean seedlings. Spm prevented the chl damage and increased its content. Exogenous Spm also modulated the endogenous free PAs level which might have the roles in improving physiological processes including antioxidant capacity, osmoregulation, and Cd and MG detoxification capacity. The overall Spm-induced tolerance of mung bean seedlings to Cd toxicity was reflected through improved growth of mung bean seedlings.

Polyamines Confer Salt Tolerance in Mung Bean (Vigna radiata L.) by Reducing Sodium Uptake, Improving Nutrient Homeostasis, Antioxidant Defense, and Methylglyoxal Detoxification Systems.

The physiological roles of PAs (putrescine, spermidine, and spermine) were investigated for their ability to confer salt tolerance (200 mM NaCl, 48 h) in mung bean seedlings (Vigna radiata L. cv. BARI Mung-2). Salt stress resulted in Na toxicity, decreased K, Ca, Mg, and Zn contents in roots and shoots, and disrupted antioxidant defense system which caused oxidative damage as indicated by increased lipid peroxidation, H2O2 content, generation rate, and lipoxygenase activity. Salinity-induced methylglyoxal (MG) toxicity was also clearly evident. Salinity decreased leaf chlorophyll (chl) and relative water content (RWC). Supplementation of salt affected seedlings with exogenous PAs enhanced the contents of glutathione and ascorbate, increased activities of antioxidant enzymes (dehydroascorbate reductase, glutathione reductase, catalase, and glutathione peroxidase) and glyoxalase enzyme (glyoxalase II), which reduced salt-induced oxidative stress and MG toxicity, respectively. Exogenous PAs reduced cellular Na content and maintained nutrient homeostasis and modulated endogenous PAs levels in salt affected mung bean seedlings. The overall salt tolerance was reflected through improved tissue water and chl content, and better seedling growth.

Effects of Sulphur Deficiency on Accumulation of K+, Na+, NO3-, PO43- and transport of K+, Na+ in mungbean (Vigna radiata L. var. BARI MUNG-6)

Sulphur deficiency caused a decrease in accumulation of Na+ but increased the accumulation of K+ in the root, stem and leaf of mungbean seedlings grown in solution culture under light bank. It also decreased K+ and Na+ accumulation in the root, stem and leaf of mungbean plants grown in sand culture under natural environmental conditions. However, sulphur deficiency increased the accumulation of NO3- in the root and stem but decreased that in leaf of mungbean seedlings grown in solution culture. It decreased accumulation of PO43- in the root, stem and leaf except an initial increase in the leaf. It increased net influx, long distance transport and transport index of K+ but decreased those of Na+ in mungbean seedlings. This result indicates that K+ is more mobile in plants than Na+.
 Dhaka Univ. J. Biol. Sci. 25(2): 123-130, 2016 (July)

Chitosan ameliorates growth and biochemical attributes in mungbean varieties under saline condition

The pot experiment was conducted to evaluate the effect of chitosan on the morphological, biochemical parameters of four Mungbean varieties (BARI Mung3, BARI Mung6, BINA Mung5 and BINA Mung8) under salinity condition. Each pot having eight kilograms of soil was prepared to grow three plants of each variety. The experiment comprised with four different conditions in triplicates viz. control, saline (40 mM NaCl, 25 days after sowing- DAS), saline plus chitosan (25 ppm chitosan, 30DAS on saline condition) and chitosan (25 ppm chitosan on control condition). Seed collections followed by data analysis were done. Proline content was measured accordingly. Salinity caused reduction in all growth and yield contributing attributes compared to control groups of all four varieties. Proline accumulation was enhanced due to saline condition, and this accumulation was not enhanced by application of chitosan. However, application of chitosan played as an outstanding stimulating role in all morphological parameters like number of flowers plant-1, number of pods plant-1, number of seeds pod-1 and thousand seeds weight under salinity stress.Res. Agric., Livest. Fish.3(1): 45-51, April 2016

Effect of flooding on growth and yield of mungbean genotypes

The field experiment was carried out with some selected mungbean genotypes viz. IPSA-13, VC-6173A, BU mug 2, BARI Mung-5 and IPSA-12 to observe the effect of 4-days flooding on their growth and yield of mungbean under field conditions at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh during September to November, 2011 maintaining 3-5 cm standing water at 24 days after emergence. Days to flowering and maturity delayed in flooded plants over control depending on the genotypes. Flooding significantly reduced Total Day Matters (TDM), number of pods per plant, seed size and seed yield of the mungbean genotypes over control. Considering higher seed yield, larger seed size and less yield reduction relative to control VC-6173A, BU mug 2 and IPSA-13 were found tolerant to soil flooding condition.Bangladesh J. Agril. Res. 41(1): 151-162, March 2016

Nitrogen fixating ability of mungbean genotypes under different levels of nitrogen application

A pot culture experiment was conducted at the Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur during kharif II, 2012 to evaluate the nodulation, biological nitrogen fixation and yield potential of genotypes of mungbean under varying levels of N application. There were 10 mungbean genotypes viz. IPSA 12, GK 27, IPSA 3, IPSA 5, ACC12890055, GK 63, ACC12890053, BU mug 4, BARI Mung 6 and Binamoog 5, each genotype treated with six levels of N (0, 20, 40, 60, 80 and 100 kg N ha-1) . Among the genotypes, the IPSA 12 at 40 kg N ha-1 produced the maximum number of nodules (14.54 plant-1) as well as the highest nitrogen fixation (2.684 mmol C2H4). This resulted in the highest seed yield (14.22 g plant-1). The genotype ACC12890053 recorded the lowest nodulation (6 plant-1), nitrogen fixation (1.134) and seed yield (7.33 g plant-1).Bangladesh J. Agril. Res. 41(1): 163-171, March 2016

Insights into spermine-induced combined high temperature and drought tolerance in mung bean: osmoregulation and roles of antioxidant and glyoxalase system.

High temperature and drought stress often occur simultaneously, and due to global climate change, this kind of phenomenon occurs more frequently and severely, which exerts devastating effects on plants. Polyamines (PAs) play crucial roles in conferring abiotic stress tolerance in plants. Present study investigated how exogenous pretreatment of spermine (Spm, 0.2mM) enhances mung bean (Vigna radiata L. cv. BARI Mung-2) seedlings tolerance to high temperature (HT, 40°C) and drought [induced by 5% polyethyleneglycol (PEG)] stress individually and in combination. Spm pretreatment reduced reactive oxygen species (ROS) production including H2O2 and O2•-, lipoxygenase (LOX) activity, and membrane lipid peroxidation (indicated by malondialdehyde, MDA) under HT and/or drought stress. Histochemical staining of leaves with diaminobenzidine and nitro blue tetrazolium chloride also confirmed that Spm-pretreated seedlings accumulated less H2O2 and O2•- under HT and/or drought stress. Spermine pretreatment maintained the ascorbate (AsA) and glutathione (GSH) levels high, and upregulated the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) which were vital for imparting ROS-induced oxidative stress tolerance under HT and/or drought stress. The cytotoxic compound methylglyoxal (MG) was overproduced due to HT and/or drought, but exogenous Spm pretreatment reduced MG toxicity enhancing the glyoxalase system. Spermine pretreatment modulated endogenous PA levels. Osmoregulation and restoration of plant water status were other major contributions of Spm under HT and/or drought stress. Preventing photosynthetic pigments and improving seedling growth parameters, Spm further confirmed its influential roles in HT and/or drought tolerance.

Effect of nitrogen on different genotypes of mungbean as affected by nitrogen level in low fertile soil

A pot experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during kharif- II season (August to November) of 2010 to find out the nitrogen acquisition and yield of mungbean genotypes affected by different levels of nitrogen fertilizer in low fertile soil. Ten mungbean genotypes viz. IPSA-12, GK-27, IPSA-3, IPSA-5, ACC12890053, GK-63, ACC12890055, BARI Mung-6, BUmug- 4 and Bina moog- 5 and six nitrogen fertilizer levels viz. 0, 20, 40, 60, 80 and 100 kg N ha-1 were included as experimental treatments. Results indicated that increasing applied nitrogenous fertilizer in low fertile soil increased nitrogen acquisition of mungbean which increased number of pods plant-1 and seeds pod-1 and finally increased yield of mungbean upto 60 kg N ha-1 irrespective of genotype and thereafter decreased. Genotype IPSA -12 produced the highest seed yield (14.22 g plant-1) at 60 kg N ha-1. The lowest yield (7.33 g plant -1) was recorded in ACC12890053 in control. From regression analysis, the optimum dose nitrogen for mungbean cultivation in the low fertile soil is 54 kg ha-1.Bangladesh J. Agril. Res. 40(4): 619-628, December 2015

Effect of nitrogenous fertilizer on yield of mungbean [Vignaradiata (L.) Wilczek] in Patuakhali district of Bangladesh

An experiment was conducted at the research field of the Horticulture Research Center at Labukhali, Patuakhali during the period from January to March 2014 to find out the most suitable BARI mungbean variety and optimum rates of N concerning higher seed yield under the regional condition of Patuakhali (AEZ–13). Two BARI mungbean varieties namely BARI mung–5 (V1) and BARI mung–6 (V2) and five levels of N fertilizer including control viz. 0 kg N ha–1 (N0), 30 kg N ha–1 (N30), 45 kg N ha–1 (N45), 60 kg N ha–1 (N60), and 75 kg N ha–1 (N75) were used for the present study as level factor A and B, respectively. In case of variety, BARI mung–6 produced significantly longest pod (7.56 cm), maximum pods (9.14) plant–1, maximum seeds (9.14) pod–1, higher weight of 100–seed (4.48 g), highest seed weight (4.33 g plant–1) and highest seed yield (1.56 t ha–1) than BARI mung–5 at harvest. In case of N fertilizer, longest pod (7.96 cm), maximum pods plant–1 (10.45), maximum seeds pod–1 (9.70), higher weight of 100–seed (4.52 g), higher weight of seed (5.73 g plant–1) and greater seed yield (1.85 t ha–1) were also obtained in 45 kg N ha–1 compare other N levels. The BARI mung–6 × 45 kg N ha–1 for seed yield was found under the regional condition of Patuakhali (AEZ-13).Asian J. Med. Biol. Res. December 2015, 1(3): 508-517

Efficacy of Herbicides in Controlling Weeds in Mungbean (<i>Vigna radiata</i> L. Wilczek) Field

A field experiment was conducted at the Pulse Research Centre of Bangladesh Agricultural Research Institute, Ishurdi, Pabna during Kharif-II season of 2010 to evaluate the efficacy of five herbicides for controlling weeds associated with mungbean (BARI Mung-6). The five herbicides such were: Paraxon (27.6% WV Paraquat dichloride salt), M-clor 5G (Butaclor), Topstar 40 WP (40% Oxadiargyl), Hammer 24 EC (Carfentrazone ethyl), and Panida 33 EC (Pendimethalin) with one control (no herbicide and also no weeding). Weed was collected species wise during weeding at 40 days after sowing from 1 m2 area of each plot and oven dried to estimate weed growth. Among the herbicides, Panida performed the best for reducing the number and dry weight of weeds. The maximum reduction of weed population, the highest weed control efficiency, seed yield (1222 kg ha-1), and maximum economic benefit were also obtained in the treatment receiving Panida 33 EC @ 2 ml L-1.The Agriculturists 2015; 13(1) 127-132

Polyamine and nitric oxide crosstalk: Antagonistic effects on cadmium toxicity in mung bean plants through upregulating the metal detoxification, antioxidant defense and methylglyoxal detoxification systems

Cadmium (Cd) contamination is a serious agricultural and environmental hazard. The study investigates cross-protection roles of putrescine (Put, 0.2mM) and nitric oxide (sodium nitroprusside; SNP, 1mM) in conferring Cd (CdCl2, 1.5mM) tolerance in mung bean (Vigna radiata L. cv. BARI Mung-2) seedlings. Cadmium stress increased root and shoot Cd content, reduced growth, destroyed chlorophyll (chl), modulated proline (Pro) and reduced leaf relative water content (RWC), increased oxidative damage [lipid peroxidation, H2O2 content, O2∙− generation rate, lipoxygenase (LOX) activity], methylglyoxal (MG) toxicity. Put and/or SNP reduced Cd uptake, increasd phytochelatin (PC) content, reduced oxidative damage enhancing non-enzymatic antioxidants (AsA and GSH) and activities of enzymes [superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST), and glutathione peroxidase (GPX)]. Exogenous Put and/or SNP modulated endogenous polyamines, PAs (putrescine, Put; spermidine, Spd; spermine, Spm), and NO; improved glyoxalase system in detoxifying MG and improved physiology and growth where combined application showed better effects which designates possible crosstalk between NO and PAs to confer Cd-toxicity tolerance.