A field experiment was carried out on February15, 2023 in the Orchard Development Project / Kerbala Agriculture Directorate, located in the Um Gharagher area / Al-Husseiniyah district / Kerbala governorate during the 2023 growing season, with the aim of studying the effect of some micronutrients and spraying with the growth regulator Brassinolide on the chemical content of the Barhi date palm, at the age of 16 years, the experiment was implemented according to a randomized complete block design (R.C.B.D) in the order of factorial experiments and with three replications. The study included two factors, the first factor was adding some macro- and micro-nutrients at three levels (0, 1000, and 1500 g palm-1), while the second factor included four concentrations of spraying with a regulator. Growth Brassinolide (0.0, 0.3, 0.6 and 0.8 mg L-1). The results showed that treatment with chemical compound fertilizer at the level of 1500 g palm-1, significantly affected the chemical content of the leaves by giving them the highest average content of chlorophyll, carbohydrates, nitrogen, phosphorus, potassium and iron (0.893 mg g-1, 12.129%, 1.001%, 0.464%, 1.303% and 168.720 mg kg-1 respectively), while the spraying treatment with brassinolide at a concentration of 0.8 mg L-1 recorded the highest average leaf content of chlorophyll, carbohydrates, nitrogen, phosphorus, potassium and iron (0.943 mg L-1, 12.420%, 1.066%, 0.497%, 1.366% and 171.880 mg kg-1, respectively). As for the interaction between the two factors, it was noted that the treatment (1500 g palm-1 + 0.8 mg L-1) was superior by giving it the highest average for all the traits under study.