Banana Research Articles

Survey on Influence of Cultural Practices on Banana Pseudostem Weevil, Odoiporus longicollis Olivier (Coleoptera: Curculionidae) Infestation in Major Banana Growing Districts of Northern Karnataka, India

The production and productivity of banana is governed by many biotic and abiotic factors. Among biotic factors, incidence of insect-pests is considered as one of the major impedimentin successful cultivation of banana. With respect to the Indian condition around eight pests commonly infest the banana crop. However, the banana pseudostem weevil Odoiporus longicollis is cosmopolite insect pest that threatens the banana cultivation. The most common management practices followed for managing this pest includes cultural practices. Diverse cultural practices have been communicated to and/or applied by resource-poor households over the past two decades to improve the overall farm health and crop tolerance to biotic/abiotic factors. Therefore, cultural practices are the only measures available to small-scale farmers for the control of banana pseudostem weevil. Hence the survey was conducted during 2020-21 to study the influence of cultural practices on O. longicollis infestation in major banana growing districts of north Karnataka. From the survey results on the cultural practices reveals that the pest severity was more severe on ratoon crop fields and the frequency recorded was 37.82%. However, the frequency recorded was 1.68% in first crop, wherein no pest infestation was recorded in main crop. Whereas, good number of farmers could not make a difference in infestation of pest between main and ratoon crop (60.50%) and in such fields infestation level was 4.11%. The banana farmers who were interacted mainly used suckers (36.13%) and tissue culture plants (36.13%) for planting followed by rhizome (24.37%), whereas few farmers used suckers and rhizomes (3.36%) for planting in a single piece of land. Accordingly, the per cent infestation of BPW was less when both tissue cultured plants (4.84%) and suckers (5.53%) used for planting. Whereas, infestation was more severe when both suckers and rhizomes were used (27.50%) on same piece of land and which was followed by rhizomes alone (16.00%).

Feasibility of Fruit Waste-Derived Media for Microbial Culture: A Sustainable and Low-cost Approach

The present study, conducted at the College of Agriculture, Latur during 2023-2024, explores the viability of using fruit waste-derived media as a cost-effective alternative to conventional media for the growth of plant pathogens. The research focuses on the growth performance of Pseudomonas fluorescens, Fusarium oxysporum f. sp. udum, and Sclerotium rolfsii on media prepared from fruit peel of eight fruits (Banana, Pineapple, Papaya, Orange, Guava, Pomegranate, Dragon fruit, and Sapota). These fruit-based media were compared to traditional Nutrient Agar (NA) and Potato Dextrose Agar (PDA). Results revealed that Sclerotium rolfsii showed maximum growth on Papaya Dextrose Agar, while Pseudomonas fluorescens exhibited the most robust growth on Banana, Papaya and Dragon Fruit Dextrose Agar. Fusarium oxysporum f. sp. udum demonstrated the highest radial growth on Banana Dextrose Agar. The study also observed that fruit-based media significantly reduced costs compared to Potato Dextrose Agar and Nutrient Agar making them a sustainable and accessible option for microbiological research, particularly in resource-limited environments.

Fusarium oxysporum and soil nutrient amendments provide short-term inhibition of Cosmopolites sordidus raising questions on biopesticide and plant nutrition potentials in tissue culture banana

Endophytic Fusarium oxysporum strain V5w2 has been suggested to offer the ecosystem service of suppressing Cosmopolites sordidus and other pests that attack tissue culture banana plants in agroecosystems. The effects of endophytic F. oxysporum V5w2 and nutrient supply on C. sordidus in potted tissue culture banana plants were investigated. In the screenhouse, rhizome damage by C. sordidus larvae was lower in F. oxysporum V5w2-inoculated plants than in non-inoculated ones. Banana plants inoculated with F. oxysporum V5w2 were larger and suffered less rhizome damage but with low chlorophyll content. Weights of C. sordidus larvae were not different between those reared on F. oxysporum V5w2-inoculated banana plants and the non-inoculated ones. Larval C. sordidus from nutrient-treated plants had lower weight than those that fed on plants that did not receive nutrients. In the field, fewer adult C. sordidus were found on F. oxysporum V5w2-inoculated banana plants than on non-inoculated plants 12 h after insect release. The number of adult C. sordidus and their eggs did not vary between F. oxysporum V5w2-inoculated banana plants and controls at the end of the experiment. Adult C. sordidus did not discriminate between nutrient-treated banana plants and those without nutrient treatment. However, non-beneficial interactions between F. oxysporum V5w2 and plant-parasitic nematodes negate the chances of its application as an endophytic biological control agent. In conclusion, while F. oxysporum V5w2 is not quite viable for application as an endophytic biological control agent for C. sordidus and other banana pests, this fungus may still have some potential to offer alternative ecosystem services through the provisioning of pest-inhibitive organic compounds.

Production of cellulose nanocrystals from the waste banana (M. Oranta) tree rachis fiber as a reinforcement to fabricate useful bionanocomposite

It is crucial to produce CNCs from the waste biomass of secondary plants to reduce the extra pressure on primary plants which have other advantageous applications in many sectors. Whereas the useless banana (M. oranta) rachis after harvesting its edible part could be a very new and beneficial one. Meanwhile, several well-known methods could be conducted, namely water retting, scouring, alkali treatment, chlorite bleaching, and acid hydrolysis, to yield high-quality CNCs. The samples of all stages were characterized by several state-of-the-art techniques, namely FTIR-ATR, TGA, FESEM, XRD, UV-vis-NIR, DLS, and zeta potential analysis, for a better understanding of their structural properties/purity. However, obtained results recommended that the CNCs have shown extensive active edges, greater thermal improvement up to 700°C, high crystallinity around 81.07±0.15% with JCPDS-ICDD card number (00-056-1718), a honeycomb-like porous microstructure, and promising spherical shapes along with an average size around 50nm. Additionally, the newly produced CNCs were free from all impurities and coloring materials and revealed a higher negatively charged surface around -45mV. Therefore, due to these outstanding features, banana rachis CNCs with a high yield (around 82.05±0.06%) would be beneficially used as promising reinforcement to fabricate useful bionanocomposite for various applications to replace fossil-based hazardous synthetic materials.

DENSITY OF INDIAN BLUE PEAFOWL (PAVO CRISTATUS) IN DIFFERENT MICROHABITATS AT THANJAVUR DISTRICT, TAMIL NADU

A total of 260 observations were taken into account to obtain thedensity of Indian Blue Peafowl (Pavo cristatus) in all the transects in the study area. This average abundance consisting of 64 Peacocks, 140 Peahens and 56 chicks were obtained from August 2023 to July 2024 (12 months). The present study area actually covering Thanjavur district (10.7870⁰ N 79.1378⁰ E)which includes the taluks of Kumbakonam, Thiruvidaimaruthur, Papanasam, Thiruvaiyaru and Thanjavur. The data on the abundance of Indian Peafowl were obtained from 57 Transects in the study area and which includes 13 micro habitats. The study area which covering the various microhabitats such paddy crop, cotton plant, bamboo, commercial flower gardens, sugar cane field, banana plant, mango tree groove, coconut tree plantation, bushes of acacia sp, palm tree ground, papaya tree, black gram plant and ground nut plant field etc.All the transects were accounted for density estimation in the study area for Indian Blue Peafowl. Totally 57 transects/areas were studied for the density (Density, Upper Confident Level and Lower Confident Level at 95%) of Indian Blue peafowl. Among the transects, the highest mean density was 8.73/km sampled (Density 8.73 UCL 11.03 and LCL 7.23) observed in Erumaipatti area. Contrary, the lowest mean density of Indian Blue Peafowl was 2.45/km sampled in Rajagiri area (Density 2.45 UCL 4.75 and LCL 0.95). The other transects/areas were recorded the moderate density for Indian Blue Peafowl.The study area covered were 13 type of microhabitats in the study area such Paddy crop, Cotton plant, Bamboo, commercial flower gardens, Sugar cane field, Banana plant, Mango tree groove, Coconut tree plantation, Bushes of Acacia sp, Palm tree ground, Papaya tree, Black gram plantation and Ground nut plant field. The density of Indian Blue fowl was 115.85/ km sampled in paddy field (Density 115.85 UCL 118.15 and LCL 114.55 recorded. The minimum density was obtained (4.38/ km sampled) in the commercial flower gardens (Mean density 4.38 UCL 6.68 and LCL 3.08.The density of Indian Blue fowl in different sex were studied and it showed the highest density was found in the Paddy field in the case of cock. The maximum density was 33.27/km sampled (Density33.27 UCL 35.57 and LCL31.97) in the paddy field. The minimum density of cock were recorded in the ground nut 1.45/km sampled (Density 1.45 UCL 3.75 and LCL 0.15).The density of Indian Blue fowl in the case of hen it was (74.91/km sampled) high (Mean density74.91 UCL 77.21 and LCL 73.61). The low density of hen was recorded (2.55/km sampled) in the commercial flower gardens (Density 2.55 UCL 4.85 and LCL 1.25).The chicks were accounted to find out the density of Indian Blue peafowls and the maximum density was 28.73/km sampled in the paddy field (Density 28.73 UCL 31.03 and LCL 27.43). The minimum density of chicks of peafowl 1.09/km sampled in the ground nut plantations (Density 1.09 UCL 3.39 and LCL 0)were obtained in the study area.

The Effect of Some Biological Fungicides and Liquid Organic Fertilizers on Sigatoka Disease on ‘Mas’ Banana Plants (Musa acuminata Colla)

Banana (Musa acuminata Colla) is a tropical fruit with great economic potential and export prospects. According to the Directorate General of Horticulture, Ministry of Agriculture, banana production from 2009 to 2014 fluctuated due to attacks by Plant Destruction Organisms (PDO), particularly Sigatoka disease caused by the fungus Mycosphaerella musciola. This study investigated biological agents like Trichoderma sp. and Gliocladium sp., along with liquid organic fertilizers, to control the disease without synthetic fungicides.The research, conducted at the University of Muhammadiyah Malang, used a split-plot design with two factors: biological pesticides (Tricogreen, Natural Glio, Trico-G) and liquid organic fertilizers (rabbit urine POC, shrimp shell MOL, banana hump MOL). The experiment had nine treatment combinations with three plant samples each. Results showed that biological pesticides significantly reduced Sigatoka disease intensity, with the J3P3 treatment (Trico-G and Banana Hump MOL) achieving the lowest infection rate of 29.55%, reducing disease intensity by 70.45%. Liquid organic fertilizers also improved plant growth, increasing leaf number and leaf area. This indicates that biological agents and organic fertilizers effectively manage Sigatoka disease and promote healthy banana plant growth.

Exploring nonanol from Bacillus velezensis (YEBBR6) for root-knot nematode management in bananas: Integrating experimental and computational approaches

Nematode infestations pose a severe threat to global banana production, leading to substantial yield losses and economic damage. Among these, the root-knot nematode Meloidogyne incognita is particularly detrimental, causing root galls that severely impair water and nutrient uptake, leading to stunted growth, reduced fruit quality, and significant economic losses for farmers. In the face of increasing agricultural challenges, bacterial endophytes have emerged as essential tools for sustainable agriculture, offering ecofriendly solutions to enhance plant health and manage pests. The present study aims to identify and characterise biomolecules from bacterial endophyte Bacillus velezensis (YEBBR6) with potential nematicidal properties against root-knot nematode Meloidogyne incognita infecting banana. The analysis of volatile and non-volatile organic compounds (VOCs/NVOCs) from the inhibition zone during the ditrophic interaction between B. velezensis (YEBBR6) and Fusarium oxysporum f.sp. cubense (Foc) revealed the presence of several distinctive biomolecules with antifungal and antimicrobial properties. These compounds included linoelaedic acid, nonanol, acetylvaleryl, 5–hydroxyl methyl furfural, clindamycin, allobarbital, 3-thiazolidine carboxamide, azulene, aminomorpholine, procyclidine, campholic acid, 3 amino-4 hydroxy phenyl sulfone, 3–deoxy mannoic lactone, hexadecanoic acid, oleic acid, and dihydroacridine. The in-silico analysis revealed that nonanol, exhibited strong binding efficacy against M. incognita protein targets, including cytochrome C oxidase subunit 1, calreticulin, neuropeptide G-protein coupled receptor, chorismate mutase 1, venom allergen-like proteins, and β-1,4-endoglucanase. Notably, nonanol proved more effective than the commercially used nematicide carbofuran 3G. Molecular dynamics studies further supported the potential of nonanol, and its nematicidal properties were validated through in vitro and pot culture studies. Moreover, nonanol was found to induce the expression of defense-related genes, such as MAPK10, WRKY22, ascorbate peroxidase, catalase, and NADPH oxidase, in banana seedlings, thus enhancing the plant's immune response. These findings highlight the potential of nonanol, derived from B. velezensis (YEBBR6), as a potent and environmentally friendly alternative for controlling nematode infestations in banana cultivation.

Exogenous dopamine ameliorates chilling injury of banana fruits during cold storage

Exogenous dopamine ameliorates chilling injury of banana fruits during cold storage

Life cycle assessment of bananas, melons, and watermelons from Costa Rica

Banana and watermelon are the two most important fruit crops in the world, and Costa Rica is one of the leading producers worldwide. Even though the importance of banana, melon, and watermelon crops, few life cycle assessments have been done and published. This study aims to provide information on the environmental performance of the banana, melon, and watermelon sectors in Costa Rica to determine the most significant parameters in the life cycle of these fruits. The supply chain of the fruit crops was analyzed as follows: farm production, post-harvest treatment and packaging, distribution and retail, household consumption, and end of life. It means that the study was developed with a cradle-to-grave approach. The functional unit was 1 kg of fresh fruit delivered in a country of the European Union. Mainly, primary data were used from farms with a conventional farming system. The fruit production process was modeled in SimaPro 9.0. ReCiPe midpoint (H) was used for the impact assessment, where 11 impact categories were evaluated, including the categories required in the product category rules for fruits and nuts. In all three fruit crops studied, the most impact stages are farm production, distribution and retail (international transport), and the end-of-life stage. In this study, waste management is relatively high due to the high amount of the non-edible part of the fruit (38.6 % for bananas and 42 % for melon and watermelon). It has a significant influence on the toxicity impact categories. The carbon footprint for the banana supply chain was estimated at 0.805 kg CO2-eq/kg of bananas produced in Costa Rica and consumed in Europe, and for melon and watermelon was 0.822 kg of CO2-eq/kg. Since there are no life cycle assessments of banana fruit with a cradle-to-grave approach, and only studies focused on carbon footprint or with a cradle-to-gate approach are available, more studies and collection of information in situ are necessary.

Agroecological Aptitude of the Northeast of Formosa (Argentinean Subtropical Region) for Banana Production Assessed by Multiple Factor Analysis

Banana (Musa spp.) is an important crop in the economies of many developing countries. In the north of Argentina, a subtropical region, banana plants grow in a suboptimal environment that limits yield because only one harvest per year is achieved. The objective of this work was to characterize the agroecological aptitude of Formosa, Argentina, for banana production through the behavior of three varieties of international use: Williams, Jaffa and Grand Naine, evaluated over five consecutive years. The three-way data analysis technique called Multiple Factor Analysis (MFA) was used for evaluating the varieties’ performances across cycles of production. The results allowed for inferring the existence of a genotype x environment interaction (GEI), corroborated by two-way factorial ANOVA. In order to determine how this suboptimal environment affected the development of each genotype of this perennial crop, Dual Multiple Factor Analysis (DMFA) was applied to jointly analyze the correlation structure between the traits that contributed to the performance of each variety in each year. The correlation structures between variables were different in each population and varied between years. All traits showed great variation between the years and genotypes, with the fruit peel thickness being the most discrepant throughout the years. However, Formosa appeared as a promising subtropical agroecological environment to produce banana because the varieties’ performances were acceptable for large-scale production systems. In addition to evaluating the adequate aptitude for cultivating banana in Formosa considering the significant effect of the GEIs, this research made a methodological contribution by proposing the use of three-way data analysis in Agronomy Science via MFA and DMFA.

High removal of volatile organic compounds on hierarchical carbons prepared from agro-industrial waste of banana fruit production for air decontamination.

Activated carbons were prepared from residues from agro-industrial banana production (banana pseudostem) and evaluated in the capture of five different volatile organic compounds (VOCs): dichloromethane, chloroform, ethyl acetate, hexane, and cyclohexane. The biomass was first submitted to a hydrothermal treatment in the presence of KOH or ZnCl2 as activating agents, followed by a dry pyrolysis. This new advance in methodology contributes to producing activated carbons with hierarchical porosity and high surface areas (701-1312 m2g-1), promoting increased interest in managing waste from banana fruit agricultural production. VOC capture studies were performed by thermal analysis, and capture capacities were similar to or higher than those presented in the literature. Higher adsorption capacities were related to the amount of available micropores, and the capture capacity was enhanced by the contribution of small mesopores. As the highest adsorbed amounts of dichloromethane (933mgg-1 at 25°C) were obtained for the material activated with ZnCl2 (1:3), further studies were carried out for this system. The experimental data was fitted using a pseudo-first-order kinetic model. A study was carried out in different atmospheres (He, N2, air), showing that co-adsorption is occurring. Under simulated environmental conditions, the capture capacity decreased slightly at equilibrium, and the new adsorbent was used for up to ten cycles without significantly losing its efficiency, indicating good application in the field.

In vitro inhibition of Xanthomonas vasicola pv. musacearum, the causal agent of banana Xanthomonas Wilt, using medicinal plant extracts from North Kivu, Eastern Democratic Republic of Congo

Banana Wilt caused by Xanthomonas vasicola pv. musacearum (Xvm), has emerged as a significant threat to food security in eastern Democratic Republic of Congo (Kivu). Currently, the only means of combatting this biotic constraint is through best agricultural practices. The aim of this study was to evaluate the effectiveness of medicinal plants used in the Kivu provinces in inhibiting Xvm. Three in vitro experiments were conducted at laboratories of Uganda's National Agriculture Research Organization (NARO) and the International Institute of Tropical Agriculture (IITA) in South Kivu. The bacterial samples were collected from infected field-grown banana plants in South Kivu and isolated on Yeast Extract Peptone Agar (YPGA). Pure Xvm colonies were used for identification via i) Polymerase Chain Reaction (PCR) with specific primers and, ii) greenhouse inoculation trials. A completely randomized design was used for the three inhibition tests (1) on Mueller Hinton Agar (MHA) using disc diffusion with 10 plant extracts; (2) in liquid YPG Broth using 10 plant extracts; and (3) on MHA using disc diffusion with 19 plant extracts. The first two trials used plant extracts diluted in petroleum ether, while the third trial used 19 plant extracts diluted in methanol. After maceration, filtration, and solvent evaporation, 10 mg of extract was diluted in 80 µl of distilled water + 10 µl of Dimethylsulfoxide (DMSO). Ten µl of this solution was impregnated on perforated discs of Whatman filter paper. Zingiber officinale (ginger) and Ricinus communis (castor) were the most effective plant extracts in suppressing Xvm. Of the thirteen plant species identified as effective against the pathogen, the Myrtaceae and Euphorbiaceae families were the most represented. Based on these results, evaluating the effectiveness of the most promising plant extracts in disinfecting the metal blades of garden tools is recommended. In addition, various phytochemical groups present in plant extracts could be evaluated for their effectiveness in suppressing Xvm, especially phenols and tannins.

Future prospects and challenges in developing saline-tolerant banana

Banana (Musa spp.) is an essential fruit with high economic value worldwide. One of the main environmental constraints in banana cultivation is abiotic stress on marginal land caused by salinity stress. This salinity stress can significantly reduce banana productivity and even cause plant death. Global climate change due to warming and reduced rainfall has increased the number of agricultural areas affected by salinity. This review article will describe current knowledge of biodiversity and various biological responses when banana plants are exposed to saline stress. This article also mentions the challenges in the banana breeding program with the current development of plant breeding and genetic engineering technology that could be used to create saline-tolerant banana accessions, its prospects, and the societal controversy underlying this technology. Thus, the information presented in this article could be a reference in the saline-tolerant banana breeding program.

Novel genetic variants of banana streak MY virus and banana streak IM virus naturally infecting banana in Northeast India.

Divergent banana streak viruses (BSV) were characterized from banana plants exhibiting diverse symptoms in the Northeast region (NER) of India. Using rolling circle amplification (RCA), the complete genome sequences of seven episomal banana streak MY virus (BSMYV) isolates, including two novel variants, and two new banana streak IM virus (BSIMV) isolates were characterized. The novel BSMYV genetic variants were associated with conspicuous necrosis on newly emerged leaves, peduncle distortion, pseudostem internal necrosis, in addition to common streak symptoms. For complete genome nucleotide sequences, BSMYV-IN4 and IN5 shared 77-79% identity with other BSMYVs, while BSMYV-IN7 and IN8 exhibited identities of 77-97%. This study reports for the first time, the complete genomes of two banana streak IM virus (BSIMV-IN1 and -IN2) infecting triploid banana hybrids exhibiting leaf distortion, stunted rosette-like growth, and necrosis, sharing 87% sequence identity with reference BSIMV genome (GenBank accession no. HQ593112). Phylogenetic inference based on complete genomes revealed the distinct and congruent placement of BSMYV-IN4 and IN5 within the BSMYV cluster. Pairwise sequence comparisons of the conserved RT/RNase H nucleotide (nt) sequences revealed that the BSMYV-IN7 and IN4 isolates showed 85% and 97% identity to BSMYV (AY805074), respectively, which shared highest nt identity with BSMYV-IN6, IN9, and IN10, at 100%. The RT/RNase H nt sequences of BSIMV-IN1 and IN2 had 98% identity with the BSIMV (HQ593112), but were characterized as novel variants of BSIMV based on complete genomes. An analysis of relative synonymous codon usage (RSCU) pattern in the ORFIII polyprotein of BSMYV and BSIMV isolates revealed AGA and AGG (arginine) as the most frequently overrepresented codons (>1.5), evolutionary conserved in the genome of both species. A total of 14 recombination events were detected among the 36 BSV genomes, with recombination breakpoints mainly located in the ORFI, III, and IGR genomic regions. A novel phylogenetic cluster, comprised of BSMYV-IN4 and IN5 within the clade I was probably derived from heterologous recombination between parents resembling banana streak VN virus (BSVNV; AY750155) and banana streak GF virus (BSGFV; KJ013507) isolates. The present study conclusively reports the infection of genetically and symptomatically distinct variants of BSMYV and BSIMV infecting banana hybrids in NER India.

Иглоподобные листовые органы хвойных. Часть II. Моделирование площади поверхности иглы

In ecological and physiological studies of plant cover, the predominant participation of leaves in the processes of photosynthesis, transpiration and respiration determines the key role of such a morphometric parameter as leaf surface area. The estimation of the area of needle-like leaf organs of conifers often requires an individual approach. The diversity of needle shapes is determined by species affiliation, morphological structure, ecological conditions and age, and, in turn, determines the diversity of methods for estimating the needle surface area. Therefore, the search for simple standard methods for determining the surface area of leaf organs of conifers is an urgent task for plant physiologists. The aim of this work has been to create a universal model for estimating the needle surface area, independent of species. To achieve it, the needle cross-section model proposed by the authors has been used, based on the transformation of the perimeter of the cross-section into an equivalent circle, the perimeter of which is associated with the parameters of the needle cross-section before the transformation. To estimate the surface area of the needle, it is possible to approximate the needle with a geometric body, which is a combination of an ellipsoid of revolution, a cone and a cylinder, with the radius of the cylinder being estimated using the needle cross-section model. The model allows to estimate the surface area of the needle by its 4 morphometric parameters: thickness, width, length of the middle part and total length. Full verification of the model proposed in the article has turned out to be impossible due to the lack of methods for accurate estimation of the needle surface area. The developed method has been compared to other methods for estimating the surface area of needle-like samples by the examples of the needles of Siberian fir (Abies sibirica L.) and common juniper (Juniperus communis L.), as well as banana fruits (Musa paradisiaca L.), and the good predictive ability of the model has been demonstrated. It can be characterized as universal with a theoretical relative error of no more than 5 %.

A review of progress on torrefaction, pyrolysis and briquetting of banana plant wastes for biofuels

A review of progress on torrefaction, pyrolysis and briquetting of banana plant wastes for biofuels

Nutritive Valorisation of Banana Tree (Musa acuminata) By-Products with Different Levels of Sodium Hydroxide

Nutritive Valorisation of Banana Tree (Musa acuminata) By-Products with Different Levels of Sodium Hydroxide

Structural and Physicochemical Characterization of Resistant Starch from Sixteen Banana Cultivars across Three Genome Groups.

Banana fruits are rich in starch, and unripe banana flour is considered a beneficial ingredient in the food industry because it has high levels of resistant starch, which significantly aids in promoting gut health and regulating blood sugar and lipid levels. However, the associations between banana cultivars with various genotypes cultivated globally and their resistant starch properties remain unclear. Herein, we investigated resistant starches from 16 banana cultivars covering three genome groups (ABB, AAB, and AAA) in order to reveal the differences and similarities among these cultivars. The results showed that there was a genotype-specific pattern in banana resistant starch (BRS) degradation. The AAA genome BRS exhibited a high degree of resistant starch degradation. The genotypes of the banana cultivars also impacted the granular morphology of the resistant starch. The ABB and AAB genome BRS were more conducive to forming resistant starch. The BRS samples from the three genome groups displayed either B-type or C-type structures. Even within the same genome group, the BRS samples exhibited differences in thermal and pasting properties. These findings reveal the impact of genotypes on BRS content and characteristics, providing a basis for future breeding and resistant starch utilization.

Banana fruit (Musa sp.) DNA-magnetite nanoparticles: Synthesis, characterization, and biocompatibility assays on normal and cancerous cells.

Magnet-mediated gene therapy has gained considerable interest from researchers as a novel alternative for treating genetic disorders, particularly through the use of superparamagnetic iron oxide nanoparticles (NPs)-such as magnetite NPs (Fe3O4NPs)-as non-viral genetic vectors. Despite their commercial availability for specific genetic transfection, such as in microglia cell lines, many potential uses remain unexplored. Still, ethical concerns surrounding the use of human DNA often impede genetic research. Hence, this study examined DNA-coated Fe3O4NPs (DNA-Fe₃O₄NPs) as potential transfection vectors for human foreskin fibroblasts (HFFs) and A549 (lung cancer) cell lines, using banana (Musa sp.) as a low-cost, and bioethically unproblematic DNA source. Following coprecipitation synthesis, DNA-Fe₃O₄NP characterization revealed a ζ-potential of 40.65 ± 4.10 mV, indicating good colloidal stability in aqueous media, as well as a superparamagnetic regime, evidenced by the absence of hysteresis in their magnetization curves. Successful DNA coating on the NPs was confirmed through infrared spectra and surface analysis results, while magnetite content was verified via characteristic X-ray diffraction peaks. Transmission electron microscopy (TEM) determined the average size of the DNA-Fe3O4NPs to be 14.69 ± 5.22 nm. TEM micrographs also showed no morphological changes in the DNA-Fe3O4NPs over a 30-day period. Confocal microscopy of HFF and A549 lung cancer cell lines incubated with fluoresceinamine-labeled DNA-Fe3O4NPs demonstrated their internalization into both the cytoplasm and nucleus. Neither uncoated Fe3O4NPs nor DNA-Fe3O4NPs showed cytotoxicity to A549 lung cancer cells at 1-50 μg/mL and 25-100 μg/mL, respectively, after 24 h. HFFs also maintained viability at 1-10 μg/mL for both NP types. In conclusion, DNA-Fe3O4NPs were successfully internalized into cells and exhibited no cytotoxicity in both healthy and cancerous cells across a range of concentrations. These NPs, capable of binding to various types of DNA and RNA, hold promise for applications in gene therapy.

Total Synthesis of (±)-Anigorootin.

An 11-step synthesis of the octacyclic-fused dimeric phenylphenalenone anigorootin (phytoalexin in banana plants) is reported. The synthetic strategy uses an electrochemical dimerization as the key step, which stereospecifically installs the four asymmetric centers. Mechanistic aspects of the dimerization process are discussed.