Bali Cattle Population Research Articles

REPRODUCTIVE PERFORMANCE OF BALI COWS IN DIFFERENT PARITIES AT AWANGPONE DISTRICT, BONE DISTRICT


 
 
 The population of Bali cattle in Bone Regency on 2021 is 275,571 cattles or 19.03% of the total population in South Sulawesi. The improvement Bali cattle genetics and increasing population is carried out by the SIWAB UP- SUS government program, that is Artificial Insemination (AI). In fact the result of AI was below of 30%, therefore needed a study was conducted to determine the reproductive performance of Bali cattle on several parity. Repro- ductive performance that is Service per Conception (S/C), Conception Rate (CR), Days Open (DO), Calving Interval (CI), and Postpartum Estrus (PPE) in different parities. The results showed that for Parity 1, Parity 2, Parity 3, and Parity 4, the respective values were for S/C ratio of 1.33, 1.16, 1.23, and 1.13; CR value of 70.00%, 86.66%, 80.00%, and 86.67%; DO value of 93.73 days, 90.33 days, 93.27 days, and 88.93 days; CI value of 367.83 days, 368.00 days, 366.30 days, and 362.77 days; and EPP value of 72.16 days, 71.86 days, 72.13 days, and 68.83 days. The analysis of reproductive performance of female cattle in this region indicates normal fertility, and the best performance was Parity 4. In conclusion, the reproductive performance of Bali cattle differs for each parity in the Awangpone Subdis- trict of Bone Regency, South Sulawesi.
 
 

Extension Performance and Satisfaction Level of Bali Cattle Farmers in South Konawe Regency, Indonesia

Objective: Extension officers play an important role in increasing community participation and are tasked with preparing, implementing, and evaluating the performance that has been carried out by the performance evaluation of extension workers in the Republic of Indonesia Minister of Agriculture Regulation No. 91/Permentan/OT.140/9/2013. Theoretical framework: The increase in livestock population is inseparable from the role of agricultural extension workers who always guide breeders to produce Bali cattle. Livestock rearing in Konawe Selatan Regency is dominated by small-scale community farms with natural and human resources that have the potential to support the development of Bali cattle. However, the development of Bali cattle in this area has not been carried out effectively. Field extension officers have held various counseling regarding cattle development, but many breeders still have not changed their farming methods. Method: The location for the study was determined by purposive sampling, considering that South Konawe Regency is an area designated by the government of the Republic of Indonesia as a source area for Bali cattle breeds. Using statistical data, the sub-district area used as the study location was determined by stratified random sampling based on the criteria for low, medium, and high population numbers of Bali cattle. Furthermore, respondents were determined by snowball sampling up to 300 farmers for each sub-district and descriptively analyzed using independent evaluation scores. Results and conclusion: The findings regarding the performance of livestock extension agents in South Konawe Regency have not given satisfaction to Bali cattle breeders, as evidenced by the analysis of the competence and role of extension agents. This study concludes that to increase the population of Bali cattle, the role of extension agents is very important, and of course, the government must further increase the competence and role of extension agents in the South Konawe Regency. Implications of the research: The findings in this study can be concluded that the competence of extension workers and the role of extension agents both in East Kolono District, Buke District, and Tinanggea District fall into the bad and not good categories according to PERMENTAN RI No. 91/Permentan/OT.140/9/2013 regarding the performance of livestock extension agents. Originality: The novelty of this research lies in evaluating the performance of extension workers by the Regulation of the Minister of Agriculture of the Republic of Indonesia No. 91/Permentan/OT.140/9/2013.

Dynamics of Bali Cattle Population in The Northern of Parigi Moutong Regency

This study aims to analyze the dynamics of the Bali cattle population in the northern part of Parigi Moutong Regency, this study has been carried out in three districts, namely Bolano, Bolano Lambunu and Moutong for two months, from March to May 2022. The method used in this study is a purposive method. sampling. The parameters observed in this study are the income rate consisting of the annual birth rate, the annual purchase rate, the annual cattle subsidy/assistance and the expenditure rate including the mortality rate (mortality), slaughter and sales as well as natural growth/Natural Increase (NI). The results showed that, the income rate for the birth rate to the mother was 54.03% and the birth rate to the population was 22.41% from 362 calves born, while the purchase rate was 6.32% from 102 heads and assistance was 6.13%. of 100 heads. The expenditure rate for the sales level is 7.18% of the 116 birds sold, while the slaughter rate is 2.48% from 40 birds, the mortality rate to the broodstock is 1.94% from 670 birds and the mortality rate to the population is 0.8% from 1615. cattle, so the number in the NI value is 21.61%.

The effect of non-genetic factors on Bali Cattle (Bos javanicus) growth traits

Abstract Bali cattle (Bos javanicus) is one of the native cattle of Indonesia, which have excellent performance though reared in hot tropic condition with low quality of feed. As generally known, livestock performance is influenced by genetic and non-genetic such as environmental factors. Therefore, this study aims to determine the non-genetic factors on growth traits of Bali cattle. The material used in this study was Bali cattle that participated in the progeny test program, consisting of 20 bulls, 122 cows and 176 calves born three years in row. The data included sex, birth season, mating system, parity, and data on growth traits which included: birth weight (BW), weaning weight at 205 days (WW205), yearling weight at 365 days (YW365) and mature weight at 730 days (MW730). Data were then analyzed using Analysis of Variance (ANOVA). The result shows that BW, YW365 and MW730 were influenced by birth season and sex (p<0.05), and WW205 was influenced by sex and parity (p<0.05). Based on the result, we concluded that the performance of the Bali cattle population in this study was influenced by non-genetic factors that are birth season, sex and parity affect the growth traits of Bali cattle.

Genomic structure of Bali cattle based on linkage disequilibrium and effective population size analyses using 50K single nucleotide polymorphisms data

Background and aim:Bali Cattle (Bos j. javanicus) is a local breed originating in Indonesia, accounting for 32.3% of the total cattle population. To date, no studies of the genetic structure and demographic status of Bali cattle have been conducted, even though the breeding of Bali cattle has a long and unique history that is likely to have impacted its genetic diversity. Therefore, a study that used molecular breeding technologies to characterize the demography of Bali cattle would be timely. This study aimed to examine genome diversity in Bali cattle and estimate the linkage disequilibrium (LD) and effective population size (Ne) values in the cattle population.Materials and Methods:In this study, we explored the population structure and genetic diversity of Bali cattle using genomic-level analyses. Our study primarily studied cattle that had been bred in livestock breeding centers since these breeds had subsequently spread throughout Indonesia. We focused on characterizing the genetic structure, determining the level of LD present, and estimating the Ne of the Bali cattle population. The genomic data used for this study were obtained from DNA samples of 48 Bali cattle collected at the Breeding Center of Bali Cattle as well as 54 genomic samples from Bali cattle collected elsewhere in Indonesia that had been used in recent publications. This genomic dataset included exclusively 50K single nucleotide polymorphisms (SNP) array (Illumina Bovine 50SNP bead chip, Illumina, USA) data.Results:We found that the LD values of Bali cattle from the breeding center and those raised elsewhere were 0.48±0.43 and 0.39±0.40, respectively. Subsequently, the Ne value of Bali cattle from the breeding center and farmers was 151 and 96, respectively.Conclusion:Our results suggest that the selection program of the breeding center is beneficial for maintaining the genetic diversity of Bali cattle.

Identification of 19-bp indel of the Pleomorphic Adenoma Gene 1 in Bali cattle population

Pleomorphic adenoma gene 1 (PLAG1) is a zinc finger transcription factor gene located on bovine chromosome 14 (BTA14) affecting body size and reproduction traits in cattle. The objective of this study was to identify 19-bp indel of the PLAG1 gene in Bali cattle population. A total of 96 blood samples of Bali Cattle were collected from Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BPTU-HPT) Denpasar. Genomic DNA was extracted from blood samples and used to detect 19-bp indel of the PLAG1 gene using following primer pair 5’-TCCGAACAACAGGTGAGGGAGAAAT-3’ and 5’-CCACTTCAGG-GGTGCTCTAGGTTTG-3’. The polymerase chain reaction (PCR) products using DNA pool samples were sequenced to validate the PCR product and to find out novel polymorphism in Bali cattle population. The result showed that there was no variation found in Bali cattle population based on 19-bp indel of the PLAG1 gene, which is indicated by 123 bp DNA band. However, sequence analysis of the PLAG1 gene resulted in a novel single nucleotide polymorphism (SNP) at nucleotide number 32235 of the PLAG1 gene that changed guanine (G) to adenine (A). This novel SNP could be furthermore genotyped and it might be a potential candidate marker for body size and reproduction traits in Bali cattle.

Diversity of Qualitative Characteristics and Their Use to Distinguish the Origin of the Bali Cattle Population

Background: The development of local Bali cattle in ex-situ areas allow for the formation of new qualitative phenotypic characteristics. The aim of this study is to characterize Bali cattle that have been created in Jambi Province’s ex-situ environment in several of rearing areas. Methods: A research sample of 478 Bali cattle from Muaro Jambi (MJB) district, Sarolangun (SLG) district, Tanjung Jabung Timur (TJT) District, Jambi City (KJB) and PTPN VI were surveyed. The qualitative phenotype characteristic observed were pattern of head color, dominant head color, inner-ear color, forehead color, side-lip color, horn shape, body-color pattern, dominant body color, back-line color, under-belly color, sock color, rump color and tail color. The frequency and percentage of each qualitative characteristic were calculated using the PROC FREQ function of SAS software version 9.0, resulting in a cross-tabulation of the qualitative characteristics observed in Bali cattle at various locations. Then, PROC CORESP performs multiple correspondence analysis (MCA) between the qualitative characteristics of the variables. The resulting plot depicts the graphical relationship between variables. Result: Research results based on sex showed that only white lower lip and black inner ear were characteristic of the head found to be common, while black backline, light-brown underbelly, white rump and black tail hair were common characteristics for the body. Head phenotype based on district showed that white lower lip and white-black inner ear could be found in male Bali cattle while white lower lip and abnormal horn characteristic could be found in females. Body characteristic analysis showed three-color pattern to be common in male Bali cattle while black backline, white feet and white rump were common characteristics found in females. These characteristics can therefore be seen as differentiating characteristics between sex and district of origin of Bali cattle.

Performan Produksi dan Kapasitas Suplay Sapi Bali Bibit dan Potong di Kabupaten Lombok Barat, Nusa Tenggara Barat

This research was conducted in West Lombok Regency with the aim of knowing the production performance and supply capacity of Bali cattle seeds and beef in West Lombok district. The research was conducted by survey method. The research sample consisted of farmers and the livestock they kept. This research was conducted in two sub-districts and three sample villages were selected for each sub-district. The number of samples is 60 farmer respondents. The variables observed included body weight, body size, birth rate, mortality rate, calf harvest, calving period and age of rejection. The data collected were analyzed descriptively. To determine the level of production and supply capacity of Bali cattle, seeds and cuts, the data were analyzed using Dania guidelines. Structure The population of Bali cattle consists of 33.33% calves, 21.21% young and 45.45% adults with a child harvest rate of 26.97% and a mortality rate of 2.49% of the population. The average body weight of Bali cattle in West Lombok Regency, weaning calf, young and adult, respectively, is 129.65, 247.98 and 275.75 kg adults with gumba height, 99.08 weaning calves, 112.75 young and adults 118.45 cm. The supply capacity of breeder cattle and beef cattle in West Lombok Regency is 24.28% of the population, consisting of 6.51% beef cattle and 17.76% breed cattle.

Dynamics and Population Structure of Bali Cattle Partnerships Maiwa Breeding Center (MBC) in Barru Regency

This study aims to determine the population dynamics, population structure, and increase of Bali cattle population Maiwa Breeding Center partnerships in Barru Regency. This research was conducted from March to April 2020 in Barru Regency, South Sulawesi. The Reproductive Efficiency of Bali cattle was 91%. Population dynamics were only influenced by the calving, mortality, and sale of livestock, with the growth of 27% (2019) and 31% (2020). The population structure was dominated by cows, 31% (2018), 48% (2019), and 54% (2020). Natural Increase (NI) of livestock was 17% (2018), 28% (2019) and 20% (2020). The conclusion of this study is that the growth of the Bali cattle population of MBC partnerships was still low when according to calving and income because it does not reach 80% of cows based on population even shows a tendency to decrease calving. Keywords: Population of dynamics, population of structure, Bali cattle, smallholder partnerships

Detection of growth hormone (GH|MspI,GHR|AluI, Pit1|HinfI) genes polymorphism and its association with body weight of Grati-Bali Cattle (Bos sondaicus)

Bali cattle is the only native Indonesian cattle that still exist and well developed. Bali cattle are known to have adaptability and a higher percentage of carcasses than other local cattle. Molecular selection related to growth traits can be done by exploration of growth hormone genes in the population of Bali cattle. This study aims to detect the level of polymorphism of GH, GHR and Pit1 genes and their association with the body weight of Grati-Bali cattle as a first step to produce a MAS (marker assisted selection). A total of 171 DNA samples Grati-Bali cattle were collected from the Beef Cattle Research Station experiment cages and its body weight data of 2014-2018 include birth weight (BW), weaning weight (WW) and yearling weight (YW). Blood samples were isolated using a zymo extraction kit. Detection of the diversity of growth hormone genes (GH, GHR and Pit1) using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with MspI, AluI, HinfI restriction enzymes. Data analysis used Chi-Square test for genotype, allele frequencies and Hardy-Weinberg Equilibrium (HWE). Association analysis used the GLM model. The results showed that the average BW, WW and YW of Grati- Bali cattle in the study were 13.3±0.3 kg, 73.7±2.4 kg and 121.3±4.2 kg, respectively and there was no statistical difference in body weight for male and female calves. The analysis showed that GH and Pit1 genes were detected by 1 genotypes respectively, each with an allele frequency > 99% and monomorphic. GHR gene was detected 3 genotypes namely AA, AG, GG, with allele frequencies A were 0.579 and G were 0.421. The GHR gene PIC value was in the moderate category. It was concluded that the GHR|AluI gene in Bali cattle is polymorphic but has not been significantly associated with the body weight of Grati-Bali cattle.

Analysis of the Development of Bali Cattle Population in Agriculture Ecosystem of Timor Island using System Dynamics

Developing Bali cattle population in Timor Island, East Nusa Tenggara province carries a potential issue regarding cattle population. The objective of this research was to conduct an analysis and projection of the development of Bali cattle population in the agricultural ecosystem in Timor Island using system dynamics. The research was conducted from January to September 2018. The research location was determined purposively based on the characteristics of the agricultural ecosystem located in Kupang Regency and Kupang City. The research used 60 Bali cattle and involved 102 rancher respondents. Interviews and field observations were conducted to obtain primary and secondary data. Data analysis was done by building a system dynamics using Powersim and interpreting the simulation results for the next 30 years. The analysis results of the system dynamics showed that a declining population of Bali cattle over the next 19 or 25 years due to the ranchers' current behavior. The contributing factors included calf mortality that reached 29.6% per year; Bali cow mortality was up to 14.1% per year; calving interval was 22 months; traditional farm management that it is not economical; and the rate of livestock export reached 29.5% per year of the total population. Rancher management interventions and farm policies to reduce the inhibiting factors of cattle productivity are needed to maintain the population of Bali cattle in Timor Island, especially in the agricultural ecosystem.

Identification of Worm Types that Infest Bali Cattle in Bolo District

Bali cattle are the primary genetic resources of Indonesia. Besides beef cattle are used as breeds, they are also distributed to all regions in Indonesia. One of the requirements for good quality of beef cattle is to be free from parasitic diseases. Bima Regency is one of the areas of Bali cattle population centers in West Nusa Tenggara, but the Bali cattle maintenance system in Bima Regency is generally still extensive, certainly increasing the potential for diseases. The disease can cause economic losses, disrupt the health of livestock and the health of people who consume meat derived from parasitic-infected animals. This study used 50 stool samples of Bali cattle aged 5 to 12 months taken randomly. The sedimentation and flotation method was used to identify nematode worm eggs. The parameters observed were gastrointestinal parasitic morphology, and then the data were analyzed descriptively. It was found two types of parasites that infected bali cattle in Bolo Sub-district, Ascaris spp, and Trichuris sp. As caris spp infected as many as 72% of 50 bali cattle faecal samples, 72% were infected by Trichuris sp., and mixture of Ascaris spp and Trichuris spp infected 54 % of the sampled cattle. The improvement of cage management and cattle maintenance system are necessary to implement. Those are by taking into high-quality nutrition feeding, regular anthelminthic treatment, and avoiding the feeding of green forage to avoid worm infection and re-infection.

Analysis of Dynamic System Toward Governor Policy of Nusa Tenggara Timur about Quota of Beef Cattle Export (Case Study in Plantation Agroecosystem of Timor Island)

The purpose of this research is to analyze the influence of the policy of the Governor of Nusa Tenggara Timur regarding the quota of beef cattle exports to areas of a national beef consumer toward the development of the Bali cattle population on Timor Island, East Nusa Tenggara. Determination of the location by purposive sampling based on physical criteria (land area), biology (availability and population of Bali cattle), and socio-culture (policy) so that the sampling locations representing the agroecosystem of plantations in Kupang district. The material used was 57 Bali cattle and involved 107 respondents of Bali cattle farmers. Respondents were interviewed using a questionnaire of reproduction status of Bali cattle. In addition to interviews, field observations were also conducted to measure the response of Bali cattle production. This research uses primary and secondary data. Data analysis is done by building a dynamic system using Powersim and interpreting simulation results for the next 30 years.The results showed a significant decrease in the population of Bali cattle over the next 30 years due to the high mortality of calves and female parent, low reproductive status of the female, and the export of cattle from uncontrolled agroecosystems. The results of this study indicate that the beef cattle export quota currently determined by local governments needs to be reviewed. The strategy to improve the quality of Bali cattle production in plantation agroecosystems to support cattle export quotas from Timor Island namely (1) farmers and local governments in striving to increase the population of Bali cattle in the agroecosystem of plantation can be done with a technical approach including prevention of death of cattle to the lowest level (<5% per year) and limitations on cattle exports according to the ability of the region; (2) improving the reproductive quality of Bali cattle through artificial insemination or controlled mating management so that it is more efficient; and (3) the government and stakeholders need to review the policy to determined cattle export quotas and improve the reproductive status of cattle in good breeding practice.

Productivity of Bali Cattle on Peatland in Central Kalimantan

This study aims to compare the productivity of Bali cattle bred on wet peatland and on dry peatland. This research was conducted in Pulang Pisau Regency, Central Kalimantan Province on September-November 2019. The research method used were a survey, observation and measurement of calf directly in the field. The samples of the area used were four districts using purposive sampling based on the largest population of Bali cattle on dry peatland and wet peatland. The total number of Bali calves were 186 tails. The observations consist of birth weight, weaning weight, and weight gain.The data were processed using the t-test (Independent t-test) with SPSS 2.2. The results obtained that productivity of male calf and female calf were higher productivitiy in dry peatland than wet peatland.

Distribution and density of Bali Cattle in South Sulawesi Province

The purpose of research was making a map of zoning tem tamper with the distribution and density of the population of Bali cattle in the province of South Sulawesi. This research was conducted in the district; Barru, Bone and Enrekang where the district was the center of the development of pure Bali cattle breeds. Method of sampling was the multi stage sampling method. Data were analyzed descriptively which included analysis of the distribution and density by using ArchGIS. The distribution and density of Bali cattle followed the spread and density of population following the spread of humans who inhabit a region.

Genetic Variation of Calpastation Gene of Indigenous Bali Cattle (Bos javanicus) in Indonesia

Calpastatin is one of gene markers affecting meat tenderness. The study aimed to evaluate genetic variation of calpastatin (CAST) gene of Bali cattle (Bos javanicus) in lndonesia. A total of 61 samples consisting of 21 Bali cattle, 22 Ongole cattle (Bos indicus), and 18 Friesian Holstein (FH) cattle (Bos taurus) were applied. The Ongole and FH cattle were involved for breed comparison. DNA was extracted from fresh blood using a High Salt method and measured their quality by a Spectrophotometer. A 523 bp of Calpastatin gene fragment was amplified by Polymerase Chain Reaction and Restriction Fragment Polymorphism (PCR-RFLP) technique with RsaI restriction enzyme for genotyping. Result showed that two variants alleles (C and G) and three genotypes (CC, GC, GG) were found in those Bali, Ongole and FH samples. Allele G was dominant allele with the highest G allele was in Bali cattle population (0.88). The higher percentage of allele C was found in Ongole and Friesian Holstein compared to that in Bali cattle. The Ongole breed tends to have a potential source of lean meat quality. This finding identified that genetic variation of CAST gene was exist in Bali cattle and adapted cattle of Ongole and FH in Indonesian.

Genetic polymorphism of MHC DRB3 (Major Histocompatibility Complex) of Bali cattle at Maiwa Breeding Center, South Sulawesi Indonesia

One of the obstacles in the development of Bali cattle is a common disease such as Jembrana, Brucellosis or Anthrax. Breeding programs for disease resistance are an effort to produce superior livestock resistant to disease. The Major Histocompatibility Complex (MHC) is a group of loci that consists of a collection of important (major) genes that control the immune response. It is known that alleles variations of the MHC genes have a relationship with natural immunity to several diseases in cattle. The aim of this study was to identify the diversity of MHC genes in the Bali cattle population at the Maiwa Breeding Center (MBC). A total of 629 heads of Bali cattle were used as samples in this study. The MHC DRB3 gene was amplified with primers flanking the region of exon 2 using a PCR machine. The product of amplification is 284 bp in lenght. Genotyping and identification of the MHC alleles were carried out by the PCR-RFLP method using PstI restriction enzymes. The results showed that the alleles variation of the MHC gene showed that P allele frequency were 0.856 and 0.938, while the p allele were 0.145 and 0.062 in Barru and Enrekang population, respectively. Observed heterozygosity values obtained in 0.144 showed that the genetic diversity of the MHC genes in the Bali cattle population at MBC was low. The chi-square values (p<0.05) showed that the MHC gene of the Bali cattle at MBC was not in the Hardy Weinberg equilibrium and indicated the possibility of a strong selection of the MHC gene. MHC gene diversity data can be used as one of the references to develope Bali cattle at the MBC.

Rooms for genetic improvement in Indonesian Bali cattle population

Bali cattle is a species of Bos javanicus d’Alton, a local cattle in Indonesia. They are loaded with potential as meat producer and well adapted to tropical climate and limited feed resources. Studies have been made to characterize the species. This paper presents a rough estimate of the opportunity to improve the Bali cattle population genetically. Our aim is to endorse that the Bali cattle could be both superior and efficient as tropical meat producer cattle. Results shows that Bali cattle population size is decreasing for the last years with a possibility to be accompanied by genetic quality decline. However, there is hope in improving Bali cattle genetically by a proper breeding strategy. This could also be an answer to the challenge of climate change which leads to global warming; where species adaptable to such environment is more beneficial in the future.

Integrated Bali Cattle Development Model Under Oil Palm Plantation

Bali cattle have several advantages such as high fertility and carcass percentage, easy adaptation to the new environment as well. Bali cattle productivity has not been optimal yet. This is due to one of the limitation of feed resources, decreasing of grazing and agricultural land. The aim of this paper is to describe Bali cattle development integrated with oil palm plantations, which is expected to improve productivity and increase Bali cattle population. This integration model is carried out by raising Bali cattle under oil palm plantation through nucleus estate scheme model or individual farmers estates business. Some of Bali cattle raising systems have been applied in the integration of palm plantation-Bali cattle. One of the intensive systems can increase daily weight gain of 0.8 kg/head, calfcrop of 35% per year and has the potency for industrial development of feed and organic fertilizer. In the semi-intensive system, it can improve the production of oil palm fruit bunches (PFB) more than 10%, increase harvested-crop area to 15 ha/farmer and reduce the amount of inorganic fertilizer. The extensive system can produce calfcrop ³70%, improve ³30% of PFB, increase business scale ³13 cows/farmer and reduce weeding costs ³16%. Integrated Bali cattle development may provide positive added value for both, palm oil business and cattle business.

Genetic Diversity of Bali Cattle Based on Microsatellite Marker in Indonesian Breeding Centre

Genetics characterization in livestock based on microsatellite has been widely implemented including for Bali cattle in three different breeding centres in Indonesia. This study aimed to determine diversity of Bali cattle microsatellites in three breeding centers namely BPTU Bali cattle in Bali, BPT-HMT Serading Sumbawa in West Nusa Tenggara, and Village Breeding Center in South Sulawesi. The number of animals used in this study was 95 head of cattle consisted of 32 heads from BPTU Bali Province, 32 heads from BPT-HMT Serading Sumbawa, and 31 heads from Village Barru Breeding Center Barru distric. Microsatellite loci used to determine the diversity was the locus SPS115, INRA037, MM12, and ETH185 based on flourescently labeled fragment method. Data analysis of microsatellite in Bali cattle at three different locations was performed by using POPGEN 1.2, Cervus, and POPTREE2 programs. The results showed that microsatellite diversity in Bali cattle detected 32 alleles from three different locations, and there were specific alleles at each location. Average values of observed heterozygosity (Ho) and expected heterozygosity (He) were 0.418 and 0.604 respectively, while the average value of polymorphism informative content (PIC) was 0.579. The Hardy-Weinberg equilibrium in general suggested that the loci used in the Bali cattle in three populations were in equilibrium, except INRA037 and ETH185. The genetic diversity between populations of Bali cattle was 0.033 (3%), while the inbreeding coefficient index in all populations was 0.296 (29.6 %). Bali cattle phylogeny tree with three populations showed that the populations of Bali cattle in BPTU Bali and VBC Barru had close genetic distance compared to the population of Bali cattle in BPT-HMT Serading Sumbawa. The results of this study provide information that the characteristics of Bali cattle breeding centers in three locations are different, so we need a directed breeding program in each population.Key words: Bali cattle, genetics diversity, microsatellite