Bakuchiol Research Articles

Bakuchiol attenuates myocardial ischemia reperfusion injury by maintaining mitochondrial function: the role of silent information regulator 1.

Ischemia reperfusion (IR) injury (IRI) is associated with poor prognoses in the settings of both cardiac surgery and ischemic heart disease and causes mitochondrial oxidative stress and cell death. Silent information regulator 1 (SIRT1), a member of the histone deacetylase family, exerts anti-IRI effects. Bakuchiol (BAK), an analog of resveratrol and a monoterpene phenol isolated from the seeds of Psoralea corylifolia (Leguminosae), protects tissues from injury. This study was designed to investigate the protective effects of BAK treatment in the setting of myocardial IRI and to elucidate the potential mechanism of those effects. Prior to induction of IR, isolated rat hearts or cardiomyocytes were exposed to BAK in either the absence or presence of the SIRT1 inhibitors Sirtinol and SIRT1 siRNA. BAK exerted cardioprotective effects, as evidenced by the improvements noted in cardiac function following ischemia, attenuated myocardial apoptosis, and changes in several biochemical parameters (including increases in the level of the anti-apoptotic protein Bcl2, decreases in the level of the pro-apoptotic protein Bax, and decreases in the cleaved Caspase 3 level). However, Sirtinol and SIRT1 siRNA each blocked BAK-induced cardioprotection by inhibiting SIRT1 signaling. Additionally, BAK significantly increased the activities of mitochondrial succinate dehydrogenase, cytochrome c oxidase, and mitochondrial superoxide dismutase and decreased the production of malondialdehyde. These findings suggested that BAK significantly attenuated IR-induced mitochondrial oxidative damage. However, Sirtinol and SIRT1 siRNA abolished BAK-dependent mitochondrial function. In summary, our results demonstrate that BAK treatment attenuates IRI by attenuating IR-induced mitochondrial oxidative damage via the activation of SIRT1/PGC-1α signaling.

Self-micro emulsifying formulation improved intestinal absorption and oral bioavailability of bakuchiol.

Bakuchiol (BAK), isolated from the seeds of Psoralea corylifolia L., recently presents a variety of pharmacologic activities. However, the poor oral bioavailability limits its further development and clinical use. The purpose of this study was to establish a self-microemulsifying (SME) formulation for oral delivery improvement of BAK. The optimized liquid SME formulation was comprised of BAK (40%), Cremophor RH 40 (30%) and Labrasol (30%). The emulsion droplets were spherical in shape, and particle size and zeta potential were determined. The in vitro dissolution test of BAK-SME formulation illustrated faster dissolution rate than the bulk drug. The permeabilities of 40μgmL-1 BAK-SME formulation in rat intestinal segments of duodenum, jejunum, ileum and colon were 30.91×10-3, 23.61×10-3, 29.43×10-3 and 23.62×10-3 cmmin-1, respectively, exhibiting 3.99 times in duodenum, 2.59 times in ileum and 2.31 times in colon greater than BAK perfusate. The oral bioavailability of BAK-SME formulation at a dose of 150mgkg-1 was determined in rats. The Cmax and the AUC(0-24h) were 515.4ngmL-1 and 4,327.2hngmL-1, respectively, which were 1.90 fold and 1.73 fold greater than the value of BAK suspension. All these results clearly stated that BAK-SME formulation performed well-improvement on oral bioavailability of BAK.

Chemistry and Biology of Bakuchiol

Chemistry and Biology of Bakuchiol

Meroterpenes and C-Glucosylflavonoids from the Aerial Parts of Plumbago zeylanica

Two meroterpenes bakuchiol and 12-hydroxyisobakuchiol, and three C-glucosylflavonoids, saponaretin, isoorientin and isoaffinetin together with other constituents plumbagin, isoshinano-lone, xanthyletin, xanthoxyletin, psoralen, plumbagic acid, plumbagic acid butyl ester, β-sitosterol and β-sitosteryl glucoside have been isolated from the aerial part of Plumbago zeylanica. This is the first report of isolation of meroterpenes and C-glucosylflavonoids from Plumbago species.

Bakuchiol, a novel monoterpenoid

Bakuchiol, a novel monoterpenoid