Bait Boxes Research Articles

Modeling platform to assess the effectiveness of single and integrated Ixodes scapularis tick control methods

BackgroundLyme disease continues to expand in Canada and the USA and no single intervention is likely to curb the epidemic.MethodsWe propose a platform to quantitatively assess the effectiveness of a subset of Ixodes scapularis tick management approaches. The platform allows us to assess the impact of different control treatments, conducted either individually (single interventions) or in combination (combined efforts), with varying timings and durations. Interventions include three low environmental toxicity measures in differing combinations, namely reductions in white-tailed deer (Odocoileus virginianus) populations, broadcast area-application of the entomopathogenic fungus Metarhizium anisopliae, and fipronil-based rodent-targeted bait boxes. To assess the impact of these control efforts, we calibrated a process-based mathematical model to data collected from residential properties in the town of Redding, southwestern Connecticut, where an integrated tick management program to reduce I.xodes scapularis nymphs was conducted from 2013 through 2016. We estimated parameters mechanistically for each of the three treatments, simulated multiple combinations and timings of interventions, and computed the resulting percent reduction of the nymphal peak and of the area under the phenology curve.ResultsSimulation outputs suggest that the three-treatment combination and the bait boxes–deer reduction combination had the overall highest impacts on suppressing I. scapularis nymphs. All (single or combined) interventions were more efficacious when implemented for a higher number of years. When implemented for at least 4 years, most interventions (except the single application of the entomopathogenic fungus) were predicted to strongly reduce the nymphal peak compared with the no intervention scenario. Finally, we determined the optimal period to apply the entomopathogenic fungus in residential yards, depending on the number of applications.ConclusionsComputer simulation is a powerful tool to identify the optimal deployment of individual and combined tick management approaches, which can synergistically contribute to short-to-long-term, costeffective, and sustainable control of tick-borne diseases in integrated tick management (ITM) interventions.Graphical

Effects of residential acaricide treatments on patterns of pathogen coinfection in blacklegged ticks.

Medically important ixodid ticks often carry multiple pathogens, with individual ticks frequently coinfected and capable of transmitting multiple infections to hosts, including humans. Acquisition of multiple zoonotic pathogens by immature blacklegged ticks (Ixodes scapularis) is facilitated when they feed on small mammals, which are the most competent reservoir hosts for Anaplasma phagocytophilum (which causes anaplasmosis in humans), Babesia microti (babesiosis) and Borrelia burgdorferi (Lyme disease). Here, we used data from a large-scale, long-term experiment to ask whether patterns of single and multiple infections in questing nymphal I. scapularis ticks from residential neighbourhoods differed from those predicted by independent assortment of pathogens, and whether patterns of coinfection were affected by residential application of commercial acaricidal products. Quantitative polymerase chain reaction was used for pathogen detection in multiplex reactions. In control neighbourhoods and those treated with a fungus-based biopesticide deployed against host-seeking ticks (Met52), ticks having only single infections of either B. microti or B. burgdorferi were significantly less common than expected, whereas coinfections with these 2 pathogens were significantly more common. However, use of tick control system bait boxes, which kill ticks attempting to feed on small mammals, eliminated the bias towards coinfection. Although aimed at reducing the abundance of host-seeking ticks, control methods directed at ticks attached to small mammals may influence human exposure to coinfected ticks and the probability of exposure to multiple tick-borne infections.

Comparison of baiting strategies in common vole management.

Worldwide, pest rodents can cause extensive damage to agriculture, forestry, food storage, and infrastructure and pose a risk to public health and livestock due to the spread of zoonotic pathogens. In Europe, the most common pest rodent species is the common vole (Microtus arvalis). Management during periodic outbreaks largely relies on rodenticidal bait with zinc phosphide. Efficient baiting with rodenticides or possibly anti-fertility products in the future require baiting methods that allow a sufficient proportion of the population to consume an effective dose of bait. We used a bait with the quantitative marker ethyl-iophenoxic acid (Et-IPA) to evaluate baiting strategies in enclosure experiments. This wheat-based bait with Et-IPA was placed in bait boxes or directly into the tunnel system entrances in different seasons and common vole abundances. Voles were live-trapped, individually marked and blood samples were collected to relate Et-IPA blood residues to bait uptake. The percentage of animals consuming bait was not heavily affected by the baiting strategy but voles had higher Et-IPA blood residues if tunnel baiting was used in autumn and if bait boxes were used in winter. Non-reproductive as well as lighter animals tended to have higher Et-IPA blood residues than reproductive individuals, whereas sex had no effect. Population density had a negative effect on the probability of residues present as well as on Et-IPA blood concentration. The results of this study might help to improve baiting techniques to manage overabundant rodent pest species regardless of the compounds to be delivered. © 2024 Julius Kühn-Institut. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Consumption of Rodenticide Baits by Invertebrates as a Potential Route into the Diet of Insectivores.

Non-target species are commonly exposed to anticoagulant rodenticides worldwide, which may pose a key threat to declining species. However, the main pathway of exposure is usually unknown, potentially hindering conservation efforts. This study aimed to examine whether baits mixed with the biomarker rhodamine B can be used to track invertebrate consumption of rodenticides in a field environment, using this to observe whether invertebrate prey are a potential vector for anticoagulant rodenticides in the diet of insectivores such as the European hedgehog (Erinaceus europaeus). Rhodamine B baits were found to create an observable response. Uptake was negligible in captured insects; however, 20.7% of slugs and 18.4% of snails captured showed uptake of bait. Maximum temperature, distance from bait, proximity to buildings, and the addition of copper tape to bait boxes all influenced the rate of bait uptake in molluscs. Based on these data, it seems likely that molluscs could be a source of rodenticide poisoning in insectivores. This research demonstrates which prey may pose exposure risks to insectivores and likely environmental factors, knowledge of which can guide effective mitigation measures. We suggest that further investigation into using mollusc repellents around bait boxes should be considered.

Community-based integrated tick management programs: cost and feasibility scenarios.

Numerous studies have assessed the efficacy of environmentally based control methods to suppress populations of the blacklegged tick (Ixodes scapularis Say), but few of these estimated the cost of control. We estimated costs for a range of tick control methods (including habitat management, deer exclusion or population reduction, broadcast of acaricides, and use of host-targeted acaricides) implemented singly or in combination and applied to a model community comprising 320 residential properties and parklands. Using the high end for cost ranges, tick control based on a single method was estimated to have mean annual costs per household in the model community ranging from $132 for treating only forest ecotone with a broadcast synthetic acaricide to kill host-seeking ticks (or $404 for treating all residential forested habitat) to >$2,000 for deployment of bait boxes (SELECT TCS) across all residential tick habitat to treat rodents topically with acaricide to kill infesting ticks. Combining different sets of multiple methods in an integrated tick management program placed the annual cost between $508 and 3,192 annually per household in the model community, underscoring the disconnect between what people in Lyme disease endemic areas say they are willing to pay for tick control (not more than $100-150 annually) and the actual costs for tick control. Additional barriers to implementing community-based tick management programs within residential communities are discussed.

Wave diffraction and radiation studies of the array of the cylindrical bait boxes with porosities in the middle of the column wall surface under wave action

In this paper, we study theoretically wave diffraction and radiation studies of the array of cylindrical bait boxes with porosities in the middle of the column wall surface under wave action. The middle position of each cylindrical bait box column wall surface is the region with holes, and the upper and lower ends of the cylindrical bait box column wall surface are the region without holes. Under the assumption of the linear theory of potential flow, the eigenfunction expansion method, Bessel's function addition theory, and Darcy's law are used to investigate the wave diffraction and radiation of the array of cylindrical bait boxes with porosities in the middle of the column wall surface. Then, the related analytical expressions for velocity potential and free surface elevation are derived. After the validation process, a detailed parametric study is carried out. Porosity, spacing, porous area size, and incident wave angle are systematically adjusted, and their effects on wave force, added mass, radiation damping coefficient, and wave height are investigated. The related phenomena are also explored. It is found that the variation of porosity coefficient and porous area size greatly influence the cylindrical bait boxed, which can efficiently bait while reducing drag. The study results can help the designer/engineer to design the bait boxes with better structural performance and achieve more rational and better baiting.

Impacts Over Time of Neighborhood-Scale Interventions to Control Ticks and Tick-Borne Disease Incidence.

Background: Controlling populations of ticks with biological or chemical acaricides is often advocated as a means of reducing human exposure to tick-borne diseases. Reducing tick abundance is expected to decrease immediate risk of tick encounters and disrupt pathogen transmission cycles, potentially reducing future exposure risk. Materials and Methods: We designed a placebo-controlled, randomized multiyear study to assess whether two methods of controlling ticks-tick control system (TCS) bait boxes and Met52 spray-reduced tick abundance, tick encounters with people and outdoor pets, and reported cases of tick-borne diseases. The study was conducted in 24 residential neighborhoods in a Lyme disease endemic zone in New York State. We tested the hypotheses that TCS bait boxes and Met52, alone or together, would be associated with increasing reductions in tick abundance, tick encounters, and cases of tick-borne disease over the 4-5 years of the study. Results: In neighborhoods with active TCS bait boxes, populations of blacklegged ticks (Ixodes scapularis) were not reduced over time in any of the three habitat types tested (forest, lawn, shrub/garden). There was no significant effect of Met52 on tick abundance overall, and there was no evidence for a compounding effect over time. Similarly, we observed no significant effect of either of the two tick control methods, used singly or together, on tick encounters or on reported cases of tick-borne diseases in humans overall, and there was no compounding effect over time. Thus, our hypothesis that effects of interventions would accumulate through time was not supported. Conclusions: The apparent inability of the selected tick control methods to reduce risk and incidence of tick-borne diseases after years of use requires further consideration.

Rodent odour bait: A new bumble bee conservation tool to enhance nest box occupancy

Abstract Bumble bee conservation focuses on supplementing floral resources. Yet, nesting site availability is linked to bumble bee abundance. As a supplement to natural nest sites, nest boxes could be deployed and baited with synthetic lures. As queen bumble bees reportedly establish colonies in abandoned rodent burrows, we hypothesized (1) that queen bumble bees sense, and behaviourally respond to, rodent odour, and (2) that lures of synthetic rodent odour can guide spring queens to nest boxes. We collected headspace odorants from bedding soiled with urine and faeces of house mice, Mus musculus, and identified the 10 odorants that elicited responses from queen antennae. To field‐test attraction of queens to mouse excreta odorants, we tree‐mounted paired nest boxes in florally rich locations, and assigned clean and soiled bedding, respectively, to one box in each pair. Queens established colonies in 17 mouse‐scented boxes and in six unscented boxes. This 43% occupancy rate of mouse‐scented boxes represents a significant improvement over the 10% occupancy rate common for unscented boxes. In a further field experiment, we baited one box in each pair with a synthetic mouse odour lure and found that queens established colonies in 13 baited boxes and in six unbaited control boxes. Specifically, Bombus mixtus established seven colonies in baited boxes and only one colony in an unbaited box. With this proof‐of‐concept that synthetic lures can guide queens to nest boxes, we anticipate that bumble bee conservation programs will soon be able to offer both expanded floral resources and baited nest boxes readily detectable by queens.

Effects of Neighborhood-Scale Acaricidal Treatments on Infection Prevalence of Blacklegged Ticks (Ixodes scapularis) with Three Zoonotic Pathogens.

Acaricides are hypothesized to reduce human risk of exposure to tick-borne pathogens by decreasing the abundance and/or infection prevalence of the ticks that serve as vectors for the pathogens. Acaricides targeted at reservoir hosts such as small mammals are expected to reduce infection prevalence in ticks by preventing their acquisition of zoonotic pathogens. By reducing tick abundance, reservoir-targeted or broadcast acaricides could reduce tick infection prevalence by interrupting transmission cycles between ticks and their hosts. Using an acaricide targeted at small-mammal hosts (TCS bait boxes) and one sprayed on low vegetation (Met52 fungal biocide), we tested the hypotheses that infection prevalence of blacklegged ticks with zoonotic pathogens would be more strongly diminished by TCS bait boxes, and that any effects of both acaricidal treatments would increase during the four years of deployment. We used a masked, placebo-controlled design in 24 residential neighborhoods in Dutchess County, New York. Analyzing prevalence of infection with Borrelia burgdorferi, Anaplasma phagocytophilum, and Babesia microti in 5380 nymphal Ixodes scapularis ticks, we found little support for either hypothesis. TCS bait boxes did not reduce infection prevalence with any of the three pathogens compared to placebo controls. Met52 was associated with lower infection prevalence with B. burgdorferi compared to placebo controls but had no effect on prevalence of infection with the other two pathogens. Although significant effects of year on infection prevalence of all three pathogens were detected, hypothesized cumulative reductions in prevalence were observed only for B. burgdorferi. We conclude that reservoir-targeted and broadcast acaricides might not generally disrupt pathogen transmission between reservoir hosts and tick vectors or reduce human risk of exposure to tick-borne pathogens.

A Review of Tick and Tick Control Strategies in Pakistan

Background: In Pakistan, ticks are a major problem for livestock and humans. These can spread a wide range of infections including protozoan, viruses, and bacteria such as spirochetes and rickettsiae. Ticks are found in all ecological and geographic zones of Pakistan. Bovine Babesiosis and Theileriosis have been documented in Pakistan. Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral disease that affects those who work with cattle, like slaughterhouse workers, vets, and hospitals. There are at least 40 tick species, mostly Haemaphysalis, Hyalomma, and Rhipicephalus. In Pakistan, CCHF is spread by Hyalomma ticks, posing a severe risk to human health. Ticks are most prevalent in the summer (June–September) and goats rather than sheep in Pakistan. Tick-borne infections include Anaplasma, Babesia, and Theileria spp., more common in sheep than goats. In the previous sixteen years, occasional CCHF outbreaks have been documented in Pakistan, with a 24% fatality rate. Mass tick control efforts have been initiated in Punjab and Sind provinces to control tick populations and zoonotic disease spread. These tick control campaigns use a lot of Deltamethrin and Ivermectin. Deltamethrin and Ivermectin can harm the ecosystem and suggested alternate tick control approaches. Deltamethrin can damage the kidneys of insect-eating birds and disrupt the life cycle of aquatic organisms when mixed with stream water. This is because roughly 60%-80% of the whole dose is excreted by the animal and is not metabolized by the animal. Dung deterioration can be hampered by a reduction of dung beetles. Tick control methods have been used for decades worldwide. But only chemical technique is still practised in Pakistan. Each method's efficacy varies with tick number, dispersion, morphology, and host species. Results: The goal of the literature review was to discuss ticks and tick management measures used on domestic animals in Pakistan and offer new and successful techniques. This article reviews the most widely used tick management methods in Pakistan. According to studies, ticks and tick-borne diseases cause significant economic losses to livestock globally. Chemicals (acaricides) are overused to treat ticks on domestic and wild animals. Tickbots, bait boxes, vaccines, natural fauna, and bio-pesticides should be promoted and used to control ticks. Conclusion: The literature concluded that tick and tick-borne diseases are a significant cause of economic losses to the livestock throughout the globe. The chemicals (acaricides) are excessively applied against ticks on domestic and wild animals. Other new techniques like tickbot, bait boxes, the discovery of vaccines, natural fauna and biopesticides should be promoted and applied to control the ticks. These strategies will have an extreme outcome on reducing the tick population. Keywords: Ticks, Ectoparasites, Congo Haemorrhagic Fever, Wild animals, Integrated tick management, Pakistan

Sanitary measures considerably improve the management of resistant Norway rats on livestock farms.

Norway rats (Rattus norvegicus) need to be controlled to prevent transmission of pathogens and damages to stored products and material, leading to considerable economic risks and losses. Given increasing resistance in Norway rats, the most persistent, bio-accumulative and toxic anticoagulant rodenticides are widely used for management, which presents hazards to the environment especially for non-target species. We investigated how sanitary measures improved management of Norway rats on 12 paired livestock farms in a region of Germany with a high population of resistant rats for reducing application of rodenticides. We recorded food intake, and tracked activity and resistance frequency during the pre-treatment, treatment and post-treatment periods. In the post-treatment period, farms using sanitary measures had a higher control success with > 13% more bait boxes without feeding than farms not using sanitary measures. In addition, the reoccurrence of rats was delayed by 85 days. With increasing accessibility to buildings and more precise positioning of the boxes, control success improved, especially when rats could not spread from water-bearing ditches through the sewer system, and when rat-hunting animals were present. Resistant animals were more common indoors than outdoors, and there were more resistant rats recorded before and during treatment than in the post-treatment period. The control success was substantially higher and reoccurrence was delayed using sanitary measures on farms. Sanitary measures can reduce resistance indirectly due to delayed re-colonization and establishment of resistant populations inside buildings. Hence, sanitary measures help to reduce economic losses, rodenticides required for rat management and environmental risk especially in the resistance area. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Integrated Tick Management in Guilford, CT: Fipronil-Based Rodent-Targeted Bait Box Deployment Configuration and Peromyscus leucopus (Rodentia: Cricetidae) Abundance Drive Reduction in Tick Burdens.

Integrated tick management (ITM) is a comprehensive strategy used to reduce presence of ticks and their associated pathogens. Such strategies typically employ a combination of host and non-host targeted treatments which often include fipronil-based, rodent-targeted bait boxes. Bait boxes target small-bodied rodents, specifically white-footed mice (Peromyscus leucopus Rafinesque) that not only play a crucial role in the blacklegged tick (Ixodes scapularis Say (Ixodida: Ixodidae)) life cycle, but also in the transmission of numerous pathogens, primarily Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner (Spirochaetales: Spirochaetaceae), the causal agent of Lyme disease. This study aimed to determine the effect of bait box deployment configuration on tick burden reduction while also further exploring bait consumption and P. leucopus abundances as measures of bait box usage and effectiveness. Boxes were deployed on nine properties within each of six neighborhoods (n = 54) in two different configurations: grid and perimeter. Multiple factors were analyzed as potential predictors for reduction in tick burdens using a backward stepwise selection procedure. Results confirmed the perimeter configuration was a more effective deployment strategy. In addition, overall P. leucopus abundance was a significant predictor of tick burden reduction while bait consumption was not. These findings not only further support the recommended perimeter deployment configuration but provide insight into effective utilization in areas of high P. leucopus abundance. The identification of this significant relationship, in addition to configuration, can be utilized by vector control professionals and homeowners to make informed decisions on bait box placement to make sustained impacts on the I. scapularis vector and associated pathogens within an ITM framework.

Prevention of Lyme and other tickborne diseases using a rodent-targeted approach: A randomized controlled trial in Connecticut.

Tickborne diseases are an increasing public health problem in the northeastern USA. Bait boxes that apply acaricide to rodents have been shown in small field studies to significantly reduce abundance of Ixodes scapularis ticks as well as their pathogen infection rates in treated areas. The effectiveness of this intervention for preventing human tickborne diseases (TBDs) has not been demonstrated. During 2012-2016, TickNET collaborators conducted a randomized, blinded, placebo-controlled trial among 622 Connecticut households. Each household received active (containing fipronil wick) or placebo (empty) bait boxes in their yards over two consecutive years. Information on tick encounters and TBDs among household members was collected through biannual surveys. Nymphal ticks were collected from a subset of 100 properties during spring at baseline, during treatment, and in the year post-intervention. Demographic and property characteristics did not differ between treatment groups. There were no significant differences post-intervention between treatment groups with respect to tick density or pathogen infection rates, nor for tick encounters or TBDs among household members. We found no evidence that rodent-targeted bait boxes disrupt pathogen transmission cycles or significantly reduce household risk of tick exposure or TBDs. The effectiveness of this intervention may depend on scale of use or local enzootic cycles.

Do primates flexibly use spatio-temporal cues when foraging?

Foraging in seasonal environments can be cognitively demanding. Comparative studies have associated large brain size with a frugivorous diet. We investigated the ability of three semi-free-ranging primate species with different degrees of frugivory (Ntrials: Macaca tonkeana = 419, Macaca fascicularis = 197, Sapajus apella = 346) in developing a mental representation of the spatio-temporal distribution of food using foraging experiments. Forty-two boxes were fixed on trees, and each week ("season"), some of them were filled with fruits which were either highly preferred, or less preferred. Spatial (geometrical panels) and temporal (peel skin of the available fruit) cues were present at each season to indicate where (food location), what (which food) was available, and when. To test the flexible use of the cues in primate foraging behaviour, we first removed the spatial and temporal cues one at a time, and then, we manipulated the "seasonal" order of the available fruit. We compared the foraging performances in the absence and the presence of the cues and during the usual and unusual seasonal order. The average proportion of baited boxes chosen by the subjects in presence of both cues was high (between 73% and 98%) for all species. The primates seemed to remember the spatio-temporal food availability (or used other cues) because no difference was found between trials with or without our spatial and temporal cues. When the usual seasonal pattern was changed, they flexibly adjusted the feeding choice by using the provided temporal cues. We discuss these results also in view of a possible experimental bias.

Baiting location affects anticoagulant rodenticide exposure of non-target small mammals on farms.

Commensal rodents such as Norway rats (Rattus norvegicus Berk.), black rats (R. rattus L.) and house mice (Mus musculus L.) damage stored produce and infrastructure, cause hygienic problems and transmit zoonotic pathogens to humans. The management of commensal rodents relies mainly on the use of anticoagulant rodenticides (ARs). ARs are persistent and bio-accumulative, which can cause exposure of non-target species. We compared the baiting strategies to use brodifacoum (BR) in bait boxes indoors only versus in and around buildings in replicated field trials at livestock farms to assess resulting BR residues in non-target small mammals. When bait was used indoors only, the percentage of trapped non-target small mammals with BR residues as well as BR concentration in liver tissue was about 50% lower in comparison to bait application in and around buildings. These effects occurred in murid rodents and shrews but not in voles that were generally only mildly exposed. During the baiting period, BR concentration in murids was stable but decreased by about 50% in shrews. Restricting the application of BR bait to indoors only can reduce exposure of non-target species. The positive effect of this baiting strategy on non-target species needs to be balanced with the need for an effective pest rodent management within a reasonable time. More research is needed to clarify which management approaches strike this balance best.

Administration of an Orally Delivered Substrate Targeting a Mammalian Zoonotic Pathogen Reservoir Population: Novel Application and Biomarker Analysis.

Reservoir-targeted vaccines (RTVs) have the potential to be effective at breaking the transmission cycle of many tick-borne pathogens including, but not limited to, Borrelia burgdorferi, B. miyamotoi, B. mayonii, Babesia microti, and Anaplasma phagocytophilum. To determine what proportion of a wild reservoir species we could effectively target, we distributed an experimental non-RTV Rhodamine B (RhB)-coated pellet formulation devoid of nutrient supplementation using bait boxes with ad libitum access, in battery-operated time-release bait stations, and by hand broadcast. Regardless of distribution method, a total of 208 of 242 (86%) white-footed mouse (Peromyscus leucopus) captures were positive for RhB by either pelage staining or by detecting fluorescent expression in vibrissae under a microscope. In bait box locations, 91% of captured mice were RhB-positive, 89% in hand broadcast locations, and 80% in time-release station locations. Based on results, we are confident that the bait formulation was readily accepted regardless of distribution technique, reached a substantial proportion of the reservoir population, and provides an effective vehicle to deliver a range of RTVs to targeted, wild, pathogen reservoir populations.

Cost analysis of vaccination in tick-mouse transmission of Lyme disease

Lyme disease is one of the most prevalent and fastest growing vector-borne bacterial illnesses in the United States, with over 25,000 new confirmed cases every year. Humans contract the bacterium Borrelia burgdorferi through the bite of the tick Ixodes scapularis. The tick can receive the bacterium from a variety of small mammal and bird species, but the white-footed mouse Peromyscus leucopus is the primary reservoir in the northeastern United States, especially near human settlement. The tick’s life cycle and behavior depend greatly on the season, with different stages of tick biting at different times. Reducing the infection in the tick-mouse cycle may greatly lower human Lyme incidence in some areas. However, research on the effects of various mouse-targeted interventions is limited. One particularly promising method involves administering vaccine pellets to white-footed mice through special bait boxes. In this study, we develop and analyze a mathematical model consisting of a system of nonlinear difference equations to understand the complex transmission dynamics and vector demographics in both tick and mice populations. We evaluate to what extent vaccination of white-footed mice can affect Lyme incidence in I. scapularis, and under which conditions this method saves money in preventing Lyme disease. We find that, in areas with high human risk, vaccination can eliminate mouse-tick transmission of B. burgdorferi while saving money.

Analyzing the cost-effectiveness of Lyme disease risk reduction approaches.

Lyme disease (LD) is endemic in many regions of the Northeastern United States. Given the elusive nature of the disease, a systematic approach to identify efficient interventions would be useful for policymakers in addressing LD. We used Markov modeling to investigate the efficiency of interventions. These interventions range from awareness-based to behavioral-based strategies. Targeting animal reservoirs of LD using fungal spray or bait boxes did not prove to be an effective intervention. Results of awareness-based interventions, including distribution of signage, fliers, and presentations, implementable in different geographical scales, suggest that policymakers should focus on these interventions, as they are both cost-effective and have the highest impact on lowering LD risk. Populations may lose focus of LD warnings over time, thus quick succession of these interventions is vital. Our modeling results identify the awareness-based intervention as the most cost-effective strategy to lower the number of LD cases. These results can aid in the establishment of effective LD risk reduction policy at various scales of implementation.

Evaluating the effectiveness of an integrated tick management approach on multiple pathogen infection in Ixodes scapularis questing nymphs and larvae parasitizing white-footed mice.

We investigated the effectiveness of integrated tick management (ITM) approaches in reducing the burden of infection with Borrelia burgdorferi, Babesia microti, and Anaplasma phagocytophilum in Ixodes scapularis. We found a 52% reduction in encountering a questing nymph in the Metarhizium anisopliae (Met52) and fipronil rodent bait box treatment combination as well as a 51% reduction in the combined white-tailed deer (Odocoileus virginianus) removal, Met52, and fipronil rodent bait box treatment compared to the control treatment. The Met52 and fipronil rodent bait box treatment combination reduced the encounter potential with a questing nymph infected with any pathogen by 53%. Compared to the control treatment, the odds of collecting a parasitizing I. scapularis infected with any pathogen from a white-footed mouse (Peromyscus leucopus) was reduced by 90% in the combined deer removal, Met52, and fipronil rodent bait box treatment and by 93% in the Met52 and fipronil rodent bait box treatment combination. Our study highlights the utility of these ITM measures in reducing both the abundance of juvenile I. scapularis and infection with the aforementioned pathogens.

Availability and Nature of Commercial Tick Control Services in Three Lyme Disease Endemic States

In an update of earlier surveys conducted in Connecticut and New Jersey in the mid-1990s, an online survey of private commercial pest control firms engaged in residential tick control showed that the application of synthetic acaricides continues to be the primary method of control used. The carbamate and organophospate acaricides, previously the most commonly used against ticks, have given way to synthetic pyrethroids and, to a lesser extent, the use of natural product/organic acaricides. Typical costs for a single acaricide application today ($100-$200 for a 1 acre [0.4 ha] property) remain similar to those reported from the earlier surveys, although the frequency of applications and, therefore, also the overall annual cost has increased. The application habitats within residential properties, life stages targeted, and application equipment used have not changed appreciably since the mid-1990s. While most survey respondents expressed knowledge of natural product acaricides and Damminix Tick Tubes, many reported that they either did not employ or knew very little about other alternative tick control methods (including entomopathogenic fungus and topical application of acaricides to tick hosts via 4-Poster deer treatment stations or Select TCS rodent bait boxes). This suggests either a failure to adequately inform the pest management industry and their potential client base of the availability of alternate methods, and/or industry concerns about cost and effectiveness of the alternatives.