Bacterium Streptococcus Mutans Research Articles

TEST OF RESISTANCE OF ALPUKAT (Persea americana mill) FRUIT EXTRACT ON THE GROWTH OF THE Streptococcus mutans

Introduction: Streptococcus mutans is one of the normal flora living in the oral cavity, but in excessive amounts is the main causative agent of dental caries. The active compounds of alkaloids, saponins and quinones in avocado pulp extract is an antibacterial alternative that can inhibit the growth of Streptococcus mutans in the oral cavity. The aim of this research was to determine the inhibitory power that contain in avocado pulp against Streptococcus mutans . Materials and Methods: This study used 4 concentrations namely 25%, 50%, 75% and 100% with each repetition 4 times. Avocado pulp extraction was carried out by maceration method using 96% ethanol solvent. Inhibition zone testing was carried out using the Kirby baurer method. Results and Discussions: The zone of inhibition was determined using the Kruskal wallis test, which showed that there were significant differences in various concentrations against the growth of Streptococcus mutans bacteria. Avocado fruit pulp extract is able to inhibit the growth of Streptococcus mutans bacteria because avocado fruit pulp extract has active compounds that cause antibacterial activity. Active compounds of avocado fruit flesh that are efficacious as antibacterials are alkaloids, saponins and quinones. Conclusion: Avocado pulp extract has been proven to have an antibacterial effect against Streptococcus mutans with an effective concentration level of 100%. Increasing the concentration of avocado pulp extract was also shown to affect the diameter of Streptococcus mutans inhibition zone

Chemical Composition and Antimicrobial Activity of Essential Oils and Hydrosols from Oregano, Sage and Pennyroyal against Oral Pathogens

In this study, the chemical composition of essential oils (EOs) extracted from Origanum vulgare ssp. hirtum Lamiaceae, (oregano), Salvia officinalis Lamiaceae (sage), Mentha pulegium Lamiaceae (pennyroyal), and respective hydrosols (HSs) has been investigated by Gas Chromatography–Mass Spectrometry (GC-MS). The antimicrobial activity was assessed against two oral pathogens: Gram-positive bacterium Streptococcus mutans and the fungus Candida albicans by determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal concentration (MBC/MFC). Three-fold diluted solutions were dispensed into each well of a 96-well microtiter plate and, after incubation, MIC was determined by visual monitoring. The MBC/MFC was determined by transferring a small quantity of sample contained in each replicate well of the microtiter plates to appropriate culture media using a microplate replicator. The EOs of the tested herbs showed antimicrobial properties, especially the EO oil of O. vulgare, which exerted the highest antimicrobial activity. HSs of S. officinalis and M. pulegium exerted no antimicrobial activity, in contrast to oregano HS, which displayed strong antimicrobial activity. In all cases, a higher number of compounds were detected in EOs than in the corresponding HSs. The major compounds of sage EO were detected to be α-thujone (25.1%), 1,8-cineole (15.8%) and β-pinene (10.0%), while the HS was characterized by the presence of 1,8-cineole (32.6%), borneol (22.6%) and α-thujone (22.4%). Pennyroyal EO and HS consists mainly of pulegone (62.1 and 50.6%, respectively). Carvacrol was the major component present in EO (63%) and HS (97.3%) of oregano, probably contributing to the antimicrobial activity. Further research is needed in order to elucidate the antimicrobial mechanisms of specific compounds present in essential oils and hydrosols of Lamiaceae grown in Greece against oral pathogens.

Benefits of Stevia (Stevia rebaudiana B.) and Sirih (Piper betle L.) Leaves Combination Againts Dental Care

Oral health is the most important part, to improve its hygiene by maintaining teeth. Cavities are a common problem. Betel leaf as an antibacterial. Stevia leaves as bacteriostatic and bactericidal also eliminate the cause of tooth decay and gingivitis. The purpose of the study was to determine the antibacterial activity of toothpaste combined with betel leaf extract with stevia leaves against the growth of Streptococcus mutans bacteria. The study used an experimental method consisting of making betel leaf and stevia leaf extracts using the maceration method. Preparation of toothpaste with a combination of betel leaf extract with stevia leaves at a concentration of 15% with the ratio of betel leaves and stevia leaves, namely formula 1 (5%: 10%), formula 2 (7.5%: 7.5%), formula 3 (10%: 5%). Testing antibacterial activity by measuring the clear zone using the disc paper method with blood agar media. Data analysis using ANOVA and Paired T test. The results of the study obtained formula (5%: 10%) clear zone of 16.42 mm, formula (7.5%: 7.5%) of 17.24mm, formula (10%: 5%) of 17.94mm, positive control of 18.66mm, negative control of 2.75mm. Conclusion 10:5 combination toothpaste has antibacterial activity.

UJI AKTIVITAS EKSTRAK ETANOL DAUN TAPAK DARA (Catharanthus roseus L.) SEBAGAI ANTIBAKTERI Streptococcus mutans

Dental caries is a localized dental disease that damages the hard tissues of the teeth formed from the accumulation of plaque on the tooth surface. Tapak dara leaf (Catharanthus roseus L.) is known to have antibacterial activity derived from secondary metabolites, namely Triterpenoid Indole Alkaloid (TIA), flavonoids, saponins, steroids, and tannins. The purpose of the study was to analyze the activity of 96% ethanol extract of tapak dara leaves with the highest inhibitory power against Streptococcus mutans bacteria and determine chemical compounds through phytochemical tests. Antibacterial testing of 96% ethanol extract of virgin tread leaves using disc diffusion method with LDH (Width Inhibitory Power) measurement. Antibacterial tests of ethanol extract of tapak dara leaf were carried out on concentrations of 60%, 65%, 70%, and 75%. DMSO solvent 10% as a negative control and amoxicillin antibiotic 500 ppm as a positive control. The results showed that antibacterial test results at a concentration of 75% showed antibacterial activity with the highest inhibitory power averaging 6.27 mm. Phytochemical test results show that 96% ethanol extract of tapak dara leaf contains alkaloids, flavonoids, tannins, saponins, and steroids

ANTIBACTERIAL ACTIVITY TEST OF CUCUMBER PEEL EXTRACT (Cucumis sativus L.) AGAINST Streptococcus mutans BACTERIA

Background: Dental caries is a process of tooth demineralization caused by acids produced from food metabolism in bacteria. One of the main etiological factors of dental caries is the microorganisms known as Streptococcus mutans bacteria. S. mutans is one of the components of human dental plaque that is associated with sucrose diet and high caries activity. Cucumber peel extract (Cucumis sativus L.) is known to have an antibacterial activity due to the presence of secondary metabolites such as flavonoids, steroids, alkaloids, saponins and phenols found in cucumber peel. Purpose: This study aimed to determine the antibacterial activity at the lowest concentration of cucumber peel extract (Cucumis sativus L.) against Streptococcus mutans bacteria. Method: This study employed the disc diffusion method with a post-test only control group design. Streptococcus mutans bacteria were cultured on Mueller Hinton Agar media, and cucumber peel extract (Cucumis sativus L.) was applied as the antibacterial agent at concentrations of 0.01 g/ml, 0.05 g/ml, 0.1 g/ml, 0.2 g/ml, 0.4 g/ml and 0.5 g/ml with seven repetitions. Results: The results of the study showed that cucumber peel extract (Cucumis sativus L.) formed inhibition zones around the paper discs against the growth of Streptococcus mutans bacteria at concentrations of 0.4 g/ml and 0.5 g/ml. Conclusion: The concentrations of 0,4 g/ml and 0,5 g/ml are the lowest concentrations of cucumber peel extract (Cucumis sativus L.) that can inhibit the growth of Streptococcus mutans bacteria. Keywords : Antibacterial, Cucumber peel, Dental Caries, Streptococcus mutans

ANTIBACTERIAL EFFECTIVENESS OF RAMBAI (Sonneratia Caseolaris) LEAVES EXTRACT AGAINST STREPTOCOCCUS MUTANS

Background: The 2018 Riskesdas data stated that dental caries prevalence in Indonesia was 88.8%. Dental caries is a multifactorial disease characterized by hard tissue damage to the enamel, dentin, and cementum of the teeth. One of the causes of caries is the presence of a microorganism called Streptococcus mutans. Rambai leaf extract (Sonneratia caseolaris) is a typical plant of South Kalimantan with content flavonoids, tripernoids, tannins, phenols, steroids that have antibacterial properties against the growth of Streptococcus mutans which can prevent caries. Purpose: To determine the difference in the effectiveness of the antibacterial extract of rambai leaves (Sonneratia caseolaris) against bacteria Streptococcus mutans in vitro. Methods: This research was a true experimental with a post-test-only design and a control group, using six treatment groups, namely 20%, 40%, 60%, 80%, positive control (povidone iodine 1%), and negative control (aquadest) respectively. Each treatment got 3 repetitions. The antibacterial test method used in this study was the liquid dilution method to determine the Minimun Inhibitory Concentration (MIC) by using Spectrophotometer and solid dilution to determine the Minimum Bactericidal Concentration (MBC) by using tools Colony Counter. Results: Based on the results and data analysis, it can be seen that the rambai leaf extract has a MIC of 20% and a MBC of 60%. Conclusion: Rambai leaf extract (Sonneratia caseolaris) can inhibit and have antibacterial activity against the growth of Streptococcus mutans. Keywords: Antibacterial, Rambai leaf extract, Streptococcus mutans

The Effect of Red Ginger Essential Oil on Adherence of Streptococcus mutans

Streptococcus mutans is a bacterium involved in the formation of caries. Red ginger essential oil is extracted from rhizomes, has a content of natural compounds, and is used in medicine for antibacterial, anti-inflammatory, and anticancer. To prove the effect of red ginger essential oil on Streptococcus mutans bacteria. The agar diffusion method is performed to test antimicrobial activity and determine the Minimum Inhibitory Concentration (MIC) against Streptococcus mutans. Furthermore, the adherence test of Streptococcus mutans bacteria was carried out using a spectrophotometer l = 570nm. MIC red ginger essential oil against Streptococcus mutans bacteria at concentrations of 0.78% and 1.56%. In the 0.78% concentrate, an adherence value of 2.12 was obtained and in the 1.56% concentrate, an adherence value of 1.93 was obtained and 3.125% concentrate obtained an adherence value of 1.78. Red ginger essential oil has potential as an antimicrobial agent by inhibiting the adherence of Streptococcus mutans bacteria.

Anti-bacterial and anti-biofilm activities of arachidonic acid against the cariogenic bacterium Streptococcus mutans.

Streptococcus mutans is a Gram-positive, facultative anaerobic bacterium, which causes dental caries after forming biofilms on the tooth surface while producing organic acids that demineralize enamel and dentin. We observed that the polyunsaturated arachidonic acid (AA) (ω-6; 20:4) had an anti-bacterial activity against S. mutans, which prompted us to investigate its mechanism of action. The minimum inhibitory concentration (MIC) of AA on S. mutans was 25 μg/ml in the presence of 5% CO2, while it was reduced to 6.25-12.5 μg/ml in the absence of CO2 supplementation. The anti-bacterial action was due to a combination of bactericidal and bacteriostatic effects. The minimum biofilm inhibitory concentration (MBIC) was the same as the MIC, suggesting that part of the anti-biofilm effect was due to the anti-bacterial activity. Gene expression studies showed decreased expression of biofilm-related genes, suggesting that AA also has a specific anti-biofilm effect. Flow cytometric analyses using potentiometric DiOC2(3) dye, fluorescent efflux pump substrates, and live/dead SYTO 9/propidium iodide staining showed that AA leads to immediate membrane hyperpolarization, altered membrane transport and efflux pump activities, and increased membrane permeability with subsequent membrane perforation. High-resolution scanning electron microscopy (HR-SEM) showed remnants of burst bacteria. Furthermore, flow cytometric analysis using the redox probe 2',7'-dichlorofluorescein diacetate (DCFHDA) showed that AA acts as an antioxidant in a dose-dependent manner. α-Tocopherol, an antioxidant that terminates the radical chain, counteracted the anti-bacterial activity of AA, suggesting that oxidation of AA in bacteria leads to the production of cytotoxic radicals that contribute to bacterial growth arrest and death. Importantly, AA was not toxic to normal Vero epithelial cells even at 100 μg/ml, and it did not cause hemolysis of erythrocytes. In conclusion, our study shows that AA is a potentially safe drug that can be used to reduce the bacterial burden of cariogenic S. mutans.

Isolation and diagnosis of Streptococcus mutans and Streptococcus sorbinus from patients with tooth decay and gingivitis

The Streptococcus bacteria Streptococcus sobrinus and Streptococcus mutans are one of the most common types in the mouth, where tooth decay, necrosis and gingivitis are among the health problems that the Iraqi society suffers from, as the treatment of bacteria has become a challenge due to their ability to resist many antibiotics. The study aimed to isolate and diagnose Streptococcus mutans and Streptococcus sobrinus and study their resistance to a number of antibiotics, and evaluate the susceptibility of Streptococcus mutans and Streptococcus sobrinus isolates in biofilms. Using biochemical tests and phenotypic diagnostics. The study included the collection of (275) oral swabs from patients with tooth decay from the area of caries on the surface of the tooth and from cysts for infected patients and visitors at the Medical City Hospital in Baghdad Governorate with ages ranging between (6-70) years and of both sexes for the period from 1/9/2022 to 1/11/2022. the number of isolates was 6 (2.18%) belonging to Streptococcus mutans and only one isolate (0.36%) of Streptococcus sobrinus, the sensitivity of all the isolates of the bacteria under study was tested towards 10 antibiotics, and the results showed that the isolates were resistant to most of the antibiotics used in this study, and all the isolates were chosen to test their ability to form biofilms using the microtitration plate, and the results showed that most of the isolates were strong in the formation of biofilms.

Evaluation of Antioxidant, Anti-inflammatory, and Antimicrobial Activities of Raspberry Fruit Extract: An In Vitro Study.

Introduction Rubus idaeus (R. idaeus)is an edible fruit thatcontains numerous significant bioactive compounds that hold important biological properties and are categorized as nutraceuticals owing to the health benefits it imparts including decreasing the risk of chronic diseases like diabetes mellitus, cancer, heart disease, and many other diseases.The objective of the present research was to explorethe antimicrobial, antioxidant, and anti-inflammatory characteristics of the aqueous raspberry extractthrough in vitro assays. Materials and methods R. idaeus aqueous extract was prepared and examined for its antimicrobial activity against Streptococcus mutans (S. mutans) bacteria and Candida albicans (C. albicans) fungi using the agar-well diffusion method, and the antioxidant activity was evaluated using the DPPH (2, 2-Diphenyl-1-picrylhydrazyl hydrate) radical scavenging assay and the hydrogen peroxide radical scavenging assay. The anti-inflammatory activity of the prepared extract was investigated using bovine serum albumin (BSA) and egg albumin denaturation assays. Results R. idaeus fruit extract displayed strong antimicrobial activity at a higher concentration of 100 µL with a 26 mm zone of inhibition against Streptococcus mutans and 24 mm for Candida albicans. The extract showed 87.42% hydrogen peroxide free radical scavenging activity and inhibited 91.12% DPPH free radicals at the highest concentration of 50 µg/mL. The extract showed effective anti-inflammatory activity by preventing the denaturation of bovine serum albumin (80%) and egg albumin proteins (77%) at the highest concentration of 50 μg/mL.The free radical scavenging activity positively correlates with the increased concentration of the prepared extract against DPPH and hydrogen peroxide free radicals, thus showing the raspberry extract's potent antioxidant activity. Similarly, the anti-inflammatory assay result shows that the prepared raspberry aqueous extract has excellent anti-inflammatory activity by preventing the denaturation of bovine serum albumin and egg albumin protein in a dose-dependent manner. Conclusion The meticulously prepared raspberry extract exhibited noteworthy antimicrobial, antioxidant, and anti-inflammatory characteristics, and owing to its astounding therapeutic benefits it holds a tremendous promise as a natural alternative in the field of oral medicine especially in the management of oral mucosal lesions, oral potentially malignant lesions such as lichen planus and leukoplakia, candidiasis, oral cancer and oral mucositis. Further animal studies and clinical trials are recommended to fully reap the therapeutics potential of raspberry extract.

Utilization of Hydroxyapatite-Chitosan-Bay Leaf Oil (Syzygium Polyanthum W.) as Antibacterial for Streptococcus Mutants

Dental caries is a disease that can damage hard tooth tissue caused by the activity of Streptococcus mutans bacteria. Streptococcus mutans bacteria are the leading cause of dental caries disease. Efforts to prevent dental caries can be made using toothpaste, but toothpaste cannot reach areas that are difficult to reach with a toothbrush. Another method that can be used is mouthwash. This study was developed with the active ingredient compound HAp-chitosan-bay leaf oil in mouthwash to have an antimicrobial effect to prevent dental caries. This study aimed to determine the chemical physics characteristics and antibacterial activity of mouthwash preparations based on hydroxyapatite-chitosan-bay leaf oil. In this study, the active ingredients hydroxyapatite-chitosan-bay leaf oil was used with variations in the concentration of bay leaf oil, namely 0.5, 1.5, and 2.5 %. FT-IR characterization showed the presence of OH, N-H, CH, C=O, C=C, C-O, and PO43- functional groups. The pH test on the mouthwash showed compliance with the quality standard for mouthwash pH, which has a pH range of 5-7. The homogeneity test results in the mouthwash preparation showed no sediment or separation. Antibacterial activity testing showed that the HAp-chitosan-bay leaf oil mouthwash preparation inhibited the growth of Streptococcus mutans bacteria. The inhibition zone in the strong category with an average of 12.75 mm was shown in the HAp-chitosan-bay leaf oil mouthwash preparation formulation with a concentration of 2.5% bay leaf oil with the addition of sodium lauryl sulfate (SLS).

Antibacterial Effect of Starfruit Extract (Averrhoa blimbi L.) against Streptococcus mutans

Dental caries is caused by gram-positive bacteria Streptococcus mutans, which is part of the normal flora of the mouth. Herbal medicines have powerful antibacterial compounds that can be used to limit the growth of germs that cause infections. The aim of the research was to determine whether starfruit extract (Averrhoa blimbi L.) has antibacterial properties against Streptococcus mutans. This type of research uses a posttest only control group design in the laboratory. Streptococcus mutans in pure culture were used as the research sample. This study had two control groups, one with four repetitions of DMSO as a negative control and one with a positive control (0.2% chlorhexidine), and four treatment groups with starfruit extract concentrations (20%, 40%, 60%, and 80 %). The disc diffusion method was used to test the antibacterial effect. One-way ANOVA and post hoc LSD statistical tests were then used to test the results. The results showed that, for Streptococus mutans bacteria, the mean and standard deviation of the diameter of the inhibition zone of starfruit extract at concentrations of 20%, 40%, 60%, and 80% were 12.03 ± 0.33 mm, 16.70 ± 0, 48 mm, 19.98 ± 0.13 mm, 22.25 ± 0.26 mm, and 19.25 ± 0.91 mm in the positive control, but no zone of inhibition was observed in the negative control. The width of the inhibition zone in starfruit extract (Averrahoa blimbi L.) at doses of 20%, 40%, 60%, 80%, positive control, and negative control was significantly different, according to the results of the one-way ANOVA test (p=0.000; p≤ 0.05). Antibacterial efficacy against Streptococcus mutans varied significantly (p≤0.05) among all star fruit extract groups and positive and negative controls, based on LSD post hoc test findings. Based on the research results, it can be said that star fruit extract at the most effective concentration, namely 80%, has antibacterial properties against Streptococcus mutans.

Formulasi Antibakteri Mouthwash Ekstrak Etanol Daun Kirinyuh (Chromolaena odorata (L.) R.M.King & H.Rob.) Pada Bakteri Streptococcus mutans STCC 31987

Kirinyuh leaves (Chromolaeina odorata (L.) R.M.King &; H.Rob.) contain secondary metabolites in the form of flavonoids, saponins, steiroids, tannins that can inhibit the growth of antibacterial growth. Based or its antibacterial properties, kirinyuh leaf extract can be developed into mouthwash to prevent dental plaque. The purpose of this study was to determine the physical stability test of the kirinyuh leaf ethanol extract mouthwash preparation and to determine the antibacterial activity of the kirinyuh leaf ethanol extract against Streptococcus mutans ATCC 31987 bacteria, so that a formulation of the kirinyuh leaf ethanol extract mouthwash preparation could be made. Mouthwash is an antiseptic preparation that is used to clean the oral cavity and is used by gargling. The extraction used was remaceration method with 70% ethanol solvent, made in various extracts with concentrations of 10%, 20%, 30%. Further evaluation of the preparation includes : organoleptic test, pH test, viscosity test. The results of the One Way ANOVA analysis showed that there was no significant difference in the pH test, viscosity test with a p>0,05 value. Organoleptic test during 6 cycles of storage in a stable state during storage. The results showed that the ethanol extract of kirinyuh leaf mouthwash was able to inhibit the growth of Streptococcus mutans bacteria, namely in F1 the weak category was 2.43±0.81 mm, F2 was 10.21±3.56 mm the strong categori, F3 was 13.38±2.72 mm strong category.
 
 Keywords : Antibacterial, Kirinyuh leaves, Mouthwash

Effectiveness of Wuluh Starfruit Leaf (Averrhoa bilimbi Linn) Extract on The Growth Streptococcus mutans In Vitro

Background: Starfruit (Averrhoa bilimbi Linn) contains vitamin C, flavonoids, tannins, and triterpenoids. This active compound is reported to prevent the growth of bacteria, including Streptococcus mutans. Objective: This study aims to determine the effectiveness of starfruit leaf extract (Averrhoa bilimbi Linn) in inhibiting Streptococcus mutans. Method: This type of research is true experimental with a post-test-only control design, where this research uses the Kirby-Baur diffusion method with samples of Streptococcus mutans bacteria and uses wuluh leaf extract with concentrations of 50%, 25%, 12.5%, 6 .25%, 3.125%. And DMSO solution as a negative control. Results: The formation of a clear zone shows the ability of starfruit leaf extract to inhibit the growth of Streptococcus mutans, with an average inhibitory diameter of 12.05 ± 0.7188, 9.50 ± 0.3464.8, 65 ± 0.2380, 7.80 ± 0.4546, 6.78 ± 0.8016. Conclusion: Based on the research results, it can be concluded that Averrhoa bilimbi Linn can inhibit Streptococcus mutans.

Antimicrobial Activity Of Mouthwash Ethanol Extract of Curcuma purpurascens Bl. (Temu Blenyeh) as Affected by Sodium Lauryl Sulfate Concentration Variations

Oral and dental health problems are usually caused by pathogenic microbes such as Streptococcus mutans and Candida albicans. Sodium lauryl sulfate is a foamer that can remove microorganisms in the oral cavity. Using mouthwash is considered the easiest and most effective because it can reach between the teeth. Therefore, the aim of this research is to create and see the effect of varying the concentration of sodium lauryl sulfate in mouthwash preparations of ethanol extract of temu blenyeh (Curcuma purpurascens Bl.) on inhibiting the growth of microbes such as Streptococcus mutans and Candida albicans. The results of testing the antimicrobial activity of mouthwash preparations against Streptococcus mutans bacteria were classified as very weak in formulation (0), namely 3.00 mm, then in the medium category, namely in formulation (I) 5.30 mm, formulation (II) 6.66 mm, and formulation (III) 10.33 mm, while the fungus Candida albicans is classified as very weak in formulation (0), namely 4.00 mm. Then it is classified as medium with an average diameter of the inhibition zone, namely formulation (I) of 5.53 mm, formulation (II) of 8.00 mm, and formulation (III) of 8.58 mm. From these results, it can be concluded that there is an effect of using varying concentrations of sodium lauryl sulfate in each formula on the antimicrobial activity of Streptococcus mutans and Candida albicans.

Pengaruh Konsentrasi Ekstrak Biji Kakao Terhadap Diameter Zona Bening Pada Bakteri Streptococcus Mutans

Cocoa beans can inhibit the growth of pathogenic bacteria such as Streptococcus Mutans. Utilization of cocoa bean extract as an anti-bacterial inhibitor containing chemical compounds extracted in simplicia aims to increase the content in the form of alkaloids, flavonoids, tannins, saponins and polyphenols. Streptococcus Mutans is a gram-positive facultative anaerobic coccus that is usually found in the human oral cavity and one of the causes of tooth decay or cariogenic bacteria capable of creating an acidic atmosphere in the mouth and can metabolize carbohydrates. This paper describes the effect of the concentration of cocoa bean extract on the diameter of the clear zone of Streptococcus Mutans bacteria. The antibacterial test was carried out using the Mueller Hinton Agar (MHA) method, samples of cocoa bean extraction were prepared by maceration method with varying concentrations of 2.5% to 12.5% ​​at 2.5% intervals. With an interval of three hours, measurements were carried out for 48 hours of observation. The results showed that the greater the concentration of cocoa bean extract, the larger the diameter of the clear zone, with the smallest diameter (8.44 ± 1.61) mm (for a concentration of 2.5%) and the largest diameter (9.70 ± 1.66) mm (for a concentration of 12.5%).

Comparison of The Antibacterial Activity of Bentonite and Amoksisilin Against Streptococcus Mutans Causes of Caries

Streptococcus mutans is a normal flora bacteria in the oral cavity. Streptococcus mutans colonizes the oral cavity during the eruption of the first teeth, which in turn causes the teeth to experience caries. Bentonite is a clay consisting of the mineral smectite, especially montmorillonite. Montmorillonite is a member of smectite with the chemical formula (Al2O3 4 SiO2 x H2O). The high cation exchange capacity and fine particle size can inhibit bacterial growth. This study aims to compare the antibacterial activity of bentonite and amoksisilin against Streptococcus mutans. Materials and research methods divide the bentonite solution into five groups with concentrations of 5%, 10%, 20%, 40%, and 100%, and one group of amoksisilin and one control group. The solution was tested for antibacterial activity by diffusion on blood agar against Streptococcus mutans. The results showed that all bentonite solution groups differed from the amoksisilin group (p 0.05). Meanwhile, the antibacterial activity of the bentonite solution group was no different from the negative control group (p 0.05). The conclusion from this study was that there was a difference in antibacterial activity between the bentonite and amoksisilin groups, where bentonite did not show inhibition of the growth of Streptococcus mutans bacteria

Oral bacterium Streptococcus mutans promotes tumor metastasis through thrombosis formation.

Thrombosis is a well-known cardiovascular disease (CVD) complication that has caused death in many patients with cancer. Oral bacteria have been reported to contribute to systemic diseases, including CVDs, and tumor metastasis. However, whether oral bacteria-induced thrombosis induces tumor metastasis remains poorly understood. In this study, the cariogenic oral bacterium Streptococcus mutans was used to examine thrombosis invitro and invivo. Investigation of tumor metastasis to the lungs was undertaken by intravenous S. mutans implantation using a murine breast cancer metastasis model. The results indicated that platelet activation, aggregation, and coagulation were significantly altered in S. mutans-stimulated endothelial cells (ECs), with elevated neutrophil migration, thereby inducing thrombosis formation. Streptococcus mutans stimulation significantly enhances platelet and tumor cell adhesion to the inflamed ECs. Furthermore, S. mutans-induced pulmonary thrombosis promotes breast cancer cell metastasis to the lungs invivo, which can be reduced by using aspirin, an antiplatelet drug. Our findings indicate that oral bacteria promote tumor metastasis through thrombosis formation. Oral health management is important to prevent CVDs, tumor metastasis, and their associated death.

The influence of surface topography and microstructure of zirconia on initial bacterial adhesion of streptococcus mutans– in vitro study

The purpose of this research in this paper is to study the physical and chemical characteristics of the surfaces and the topography of the surfaces of zirconium materials used for the processing of fixed prosthetic works and their influence on the surface adhesion of Streptococcus mutans bacteria. The samples used for examination are produced from zircon material, they are divided into two groups, the first group after sintering are polished without glaze, while the second group, after sintering, are covered with a layer of glaze. For the realization of the experimental part of this research, the following methods and analyzes were used: surface roughness measurement (SR), contact angle measurement, surface free energy determination (SFE), biofilm formation and preparation for scanning with a microscope electronic (SEM) Specimens produced from zirconia, polished but unglazed showed higher contact angle values 90.340, while glazed samples showed lower values than unglazed ones 61.640, Surface free energy measurements of zirconia samples polished without glaze showed significantly lower values (24.31mJ/m2 ) compared to the values obtained in the zircon samples covered with glaze (40.66mJ/m2 ), The surface roughness values are higher in unglazed zirconia samples and is 0.65μm, while in the polished and glazed zirconia samples, the roughness of the surfaces is less 0.29μm, SEM analysis results show that the smallest number of attached bacteria is found on the surfaces of samples with unglazed zircon (8 bacteria), while in samples with zircon covered with glaze, a much larger number of bacteria (26 bacteria) were found on their surfaces. The roughness of the surfaces was shown to have no effect on the bacterial surface adhesion of these samples.

Evaluation of Antibacterial Efficacy of Cinnamomum Burmanii Leaf Essential Oil in Toothpaste Formulation Against Streptococcus Mutans: An Experimental Study

Background: Traditional cinnamon bark harvesting practices often discard valuable leaves, neglecting their potential benefits such as essential oil with antibacterial properties. Previous studies have highlighted the antibacterial effects of Cinnamomum burmannii leaves from Indonesia, including their potential as antibacterial agents. The study aimed to evaluate the antibacterial effect of Cinnamomum burmanii leaf essential oil in toothpaste formulation against Streptococcus mutans
 Method: This quasi-experimental study compared the antibacterial effectiveness of toothpaste containing essential oil with Streptococcus mutans cultures in Brain-heart Infusion Broth (BHIB) overnight. Six wells were created on agar plates using sterile 6 mm punches. Each well was filled with the following: Positive control (Chlorhexidine), pure essential oil, 100% essential oil toothpaste, 75% diluted essential oil toothpaste, 50% diluted essential oil toothpaste, and negative control (aquadest). The diameter of inhibition zones was measured after incubating all plates at 36°C for 24 hours.
 Result: The inhibition zone diameter was measured to evaluate the effectiveness of essential oil toothpaste against dental plaque bacteria. Non-parametric Kruskal-Wallis statistical analysis revealed no significant difference in the diameter produced between Cinnamomum zeylanicum leaf essential oil and the positive control minosep (p=0.10). However, after formulation into toothpaste, a significant decrease in effectiveness was observed (p=0.015).
 Conclusion: It can be concluded that cinnamon leaf essential oil is effective in inhibiting the growth of Streptococcus mutans bacteria. However, when formulated into toothpaste, its effectiveness decreases with lower concentrations of the toothpaste dilution.