Bacterium Pseudomonas Fluorescens Research Articles

Inoculation With Growth-Promoting Bacteria Associated With the Reduction of Phosphate Fertilization in Sugarcane

Sugarcane holds the prominent position in global economy. Extensive research is needed to enhance the efficiency of phosphorous fertilizers which increase the cost of sugarcane production more specifically in the less fertile soils which are weathered and phosphorous deficit as tropical region soils. We are currently living in a transitional period called the "Green Micro-revolution", in which the use of plant growth-promoting bacteria (PGPB) is justified and although bacteria such as A. brasilense, Bacillus and P. fluorescens in other crops are recognized for increasing production however, little is known about the effects of these microorganisms on sugarcane. The current study aimed to evaluate the effect of inoculation with three species of plant growth promoting bacteria (Azospirillum brasilense, Bacillus subtilis and Pseudomonas fluorescens) without or with combine application of reduced doses of phosphate fertilizer, in the P soil available, P leaf concentration, shoot yield and P accumulation in sugarcane (cane-plant) at the end of the cycle. The experiment was carried out with a sugarcane crop in a Hapludox Rhodic with low available P content, in a randomized block design with 8 x 5 factorial scheme, being eight inoculations and five P doses (0, 45, 90, 135 and 180 kg ha-1 P2O5) as triple superphosphate. The bacterial inoculations influenced the leaf P content, so that inoculation with B. subtilis + P. fluorescens provided the highest concentration of phosphorus in the sugarcane leaf. Both the layers of the soil were differently influenced by inoculation and P doses whereas inoculation with A. brasilense + P. fluorescens at 135 kg P2O5 ha-1 provided the highest soil P content in the soil layer of 0-0.25 m. Although, maximum stalk yield was obtained with inoculation of B. subtilis + P. fluorescens at the dose of 135 kg ha-1 P2O5. The inoculation of A. brasilense + B. subtilis with application of 45 kg P2O5 ha-1 improved dry matter, total P accumulation and stalk production by 38% in sugarcane variety (RB92579) and reduced P fertilization by 75% for the same variety grown in low-P soil.

Disentangling the mechanisms underpinning disturbance-mediated invasion.

Disturbances can play a major role in biological invasions: by destroying biomass, they alter habitat and resource abundances. Previous field studies suggest that disturbance-mediated invader success is a consequence of resource influxes, but the importance of other potential covarying causes, notably the opening up of habitats, have yet to be directly tested. Using experimental populations of the bacterium Pseudomonas fluorescens, we determined the relative importance of disturbance-mediated habitat opening and resource influxes, plus any interaction between them, for invader success of two ecologically distinct morphotypes. Resource addition increased invasibility, while habitat opening had little impact and did not interact with resource addition. Both invaders behaved similarly, despite occupying different ecological niches in the microcosms. Treatment also affected the composition of the resident population, which further affected invader success. Our results provide experimental support for the observation that resource input is a key mechanism through which disturbance increases invasibility.

Genome-wide analysis of NBS-encoding resistance genes in the Mediterranean olive tree (Olea europaea subsp. europaea var. europaea): insights into their molecular diversity, evolution and function

The olive tree is an economically important fruit tree, widely spread in the Mediterranean Basin. The recent availability of two full genomes of this plant offers an opportunity to characterize its disease resistance gene repertoire. The nucleotide-binding site (NBS) gene family accounts for the largest number of known disease resistance genes and is one of the largest gene families in plant genomes. Here, we have identified 270 regular NBS-type genes in the olive genome, roughly representing 0.5% of whole-genome protein-coding genes in this species. A systematic characterization of this gene set was conducted on the bases of conserved protein signatures, gene duplications, phylogenetic relationships, selection pressure, and expression evidence. Several structural features were particularly pronounced in O. europaea, including a very small proportion of TIR-type NBS genes, as well as numerous non-canonical functional domains associated to the NBS. Analyses of duplication and selection pressure strongly suggest that recent duplication, in conjunction with positive selection, played a remarkable role in the evolution of NBS-encoding genes in olive. Based on the phylogenetic tree produced, three pairs of possible orthologs between the olive NBS genes (OeNBS) and those from Arabidopsis thaliana, Oryza sativa Indica, and O. sativa Japonica were identified and could be of interest to characterize the uncharacterized function of the OeNBS genes in olive. Various expression patterns of olive NBS-encoding genes in different tissues were observed using expressed sequence tags (ESTs). Interestingly, we identified a pair of duplicated NLRs integrating an N-terminal RPW8 domain, which are possibly expressed in response to Verticillium dahliae, in addition to a gene encoding C-terminal Armadillo repeat domain, which is possibly induced upon colonization by the endophytic bacterium Pseudomonas fluorescens. This work is the first to characterize NBS family members and report on their architectural, evolutionary, and functional features. Our findings lay an important multifaceted foundation for a deeper understanding of R genes biology in olive tree and provide valuable information for further functional studies.

PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) DARI AKAR BAMBU APUS (Gigantochola apus) MENINGKATKAN PERTUMBUHAN TANAMAN

Abstract: Living things need nutrients to grow and their endurance. The presence of nutrients around plant roots is very important for microorganisms as biological control agents and also for plant pathogens. PGPR Plant Growth Promoting Rhizobacteria (PGPR) has a role in increasing plant growth, crop yields and plant growth. Bacteria in PGPR can actively colonize the rhizosphere. Bamboo roots are colonized by many bacteria (Pseudomonas fluorescens), these bacteria can play a role in increasing the solubility of P in the soil, certain strains of Pseudomonas sp. can prevent plants from fungal pathogens that come from the soil. Rhizobacteria from the Bacillus spp. and Pseudomonas spp., able to dissolve phosphate, while the Serratia spp. group, in addition to being able to increase the availability of P (phosphate) can also fix nitrogen and be able to synthesize IAA. Microbes with the ability to produce phytohormone are an effort to help in increasing plant growth.

Biological protection of plum from shot hole disease in the humid subtropics of the Krasnodar region (Russia)

The article considers the biologization of plum trees protection system from shot hole disease caused by the phytopathogenic fungusWilsonomyces carpophilusin the humid subtropics of the Krasnodar region. Several biological fungicides based on different strains of the bacteriumBacillus subtilis(Alirin-B®, Bactophyt®, Gamair®, Phytosporin-M®, Vitaplan®), the bacteriumPseudomonas fluorescens(Rhizoplan®) and the fungusTrichoderma harzianum(Glyocladin® and Trichocin®) were tested on Stanley plum trees at the experimental base of the Federal Research Centre the Subtropical Scientific Centre of the Russian Academy of Sciences (Sochi) in 2015–2017. According to seasonal features of shot hole disease and the results of experiments, the optimal timetable schedule for plum treatment with the fungicides was found (3 treatments in May–June).

Effect of various biological control agents (BCAs) on drought resistance and spring barley productivity

The aim of the article is to study the effect of various biological agents of biofungicides on plant resistance to drought and formation of spring barley crops. Endophytic bacteria (Bacillus subtilis), rhizospheric bacteria (Pseudomonas fluorescens), actinomycetes (Streptomyces sp.), and micromycetes (Trichoderma viride) were used as bioagents of biofungicides. Preparations based on biological agents were used to treat spring barley seeds. The studies were carried out in laboratory and field conditions. In the conditions of the model artificial acute drought, it was found that seed treatment with Streptomyces sp. Stimulates the leaf and root growth, as well as increases peroxidase activity in the leaves. The maximum content of proline was during seed treatment with Pseudomonas fluorescens. An increase in the content of chlorophyll was observed when treating with Trichoderma viride seeds. In the field conditions, Pseudomonas putida and Streptomyces were more efficient by the content of proline in seedlings. In terms of the spring barley yield, seed treatment with Streptomyces sp., Pseudomonas putida and Bacillus subtilis was more efficient. Streptomyces sp., Pseudomonas putida and Bacillus subtilis are promising for increasing barley resistance to drought.

Mixing and matching genes of marine and terrestrial origin in the biosynthesis of the mupirocin antibiotics.

With growing understanding of the underlying pathways of polyketide biosynthesis, along with the continual expansion of the synthetic biology toolkit, it is becoming possible to rationally engineer and fine-tune the polyketide biosynthetic machinery for production of new compounds with improved properties such as stability and/or bioactivity. However, engineering the pathway to the thiomarinol antibiotics has proved challenging. Here we report that genes from a marine Pseudoalternomonas sp. producing thiomarinol can be expressed in functional form in the biosynthesis of the clinically important antibiotic mupirocin from the soil bacterium Pseudomonas fluorescens. It is revealed that both pathways employ the same unusual mechanism of tetrahydropyran (THP) ring formation and the enzymes are cross compatible. Furthermore, the efficiency of downstream processing of 10,11-epoxy versus 10,11-alkenic metabolites are comparable. Optimisation of the fermentation conditions in an engineered strain in which production of pseudomonic acid A (with the 10,11-epoxide) is replaced by substantial titres of the more stable pseudomonic acid C (with a 10,11-alkene) pave the way for its development as a more stable antibiotic with wider applications than mupirocin.

The enemy within the gut: bacterial pathogens in celiac autoimmunity.

Microbially derived peptides that mimic immunogenic gliadin peptides in celiac disease have been identified using a structure- and sequence-guided search. T cell activation assays and crystal structures reveal that a protein from the commensal bacterium Pseudomonas fluorescens can be processed and presented by antigen-presenting cells to potently activate T cells from patients with celiac disease.

UJI EFEKTIFITAS AGENSIA HAYATI DALAM MENGENDALIKAN PENYAKIT LAYU BAKTERI Ralstonia solanacearum DAN MENINGKATKAN PERTUMBUHAN SERTA HASIL TANAMAN CABAI (Capsicum annum)

Chili is an important commodity for most people, because of its function in terms of improving taste and appetite. Chili was seriously developed with the support of government funds for chili farmers. The development of chili plants, has enough obstacles, especially wich caused by plant disturbing organisms whether it is pests or diseases. The important organism that disturbs chili is wilting caused by Ralstonia solanacearum. In South Kalimantan, this disease reportedly attacked chilies spread in the districts of Banjar, Banjarbaru, Barito Kuala, Tapin, Hulu Sungai Tengah and Balangan with a cumulative attack area of 15,7 ha on 2017 (South Kalimantan BPTPH Database). This disease is important because of the nature of the attack which can cause the plants to wilt suddenly all part of the plant. Plants wich attacked by bacterial wilt cannot be cured. The effort that can be done in prevention before pathogens infect the plants (Sastra, 2004). One way to overcome this problem is biological control using biological agents from the type of antagonistic bacteria Bacillus spp, Pseudomonas fluorescens, and type of antagonistic fungus Trichoderma spp. From three biological agents proven able to inhibit the development of R. solanacearum in vitro which produces a zone of inhibition as wide as 13,5 mm for Bacillus spp, 22,25 mm for Pseudomonas fluorescens and 8,42 mm for Trichoderma spp. and can increase plant height growth, chili weight, root weight, wet weight, and dry weight plants. Trichoderma spp as the best biological agents for increasing chili growth and yield.

Weed-Suppressive Bacteria Have No Effect on Exotic or Native Plants in Sagebrush-Steppe

Approaches and techniques for control of exotic annual grasses are a high priority in rangelands including sagebrush steppe. Strains of the soil bacterium Pseudomonas fluorescens have been proposed to be selectively pathogenic to multiple species of exotic annual grasses (“Pf,” weed-suppressive bacteria, “WSB”). However, defensible tests of the target and nontarget effects of these WSB strains in the field are needed. We evaluated the effects of D7 and MB906 strains of Pf WSB in sagebrush steppe invaded by cheatgrass (Bromus tectorum L), medusahead (Taeniatherum caput-medusae L. Nevski), and other exotic annual grasses. We evaluated the WSB strains with and without herbicides (imazapic, rimsulfuron) or discing to mix surface-spray of the WSB into deeper soils, and we replicated these tests in three ecoregions that differed in soils and climate. Over 3 yr after treatment, neither WSB strain affected cover of exotic annual grasses, perennial bunchgrasses, or the total community, either with WSB alone or in combination with herbicides or discing. WSB has received considerable attention and is being applied across large rangeland areas, but the WSB strains and methods applied here were ineffective. We recommend any future use of WSB be applied in an experimental fashion, with experimental design and measurement of responses, until its effects can be proven.

Comparative Analysis of the Conversion of Mandelonitrile and 2-Phenylpropionitrile by a Large Set of Variants Generated from a Nitrilase Originating from Pseudomonas fluorescens EBC191

The arylacetonitrilase from the bacterium Pseudomonas fluorescens EBC191 has been intensively studied as a model to understand the molecular basis for the substrate-, reaction-, and enantioselectivity of nitrilases. The nitrilase converts various aromatic and aliphatic nitriles to the corresponding acids and varying amounts of the corresponding amides. The enzyme has been analysed by site-specific mutagenesis and more than 50 different variants have been generated and analysed for the conversion of (R,S)-mandelonitrile and (R,S)-2-phenylpropionitrile. These comparative analyses demonstrated that single point mutations are sufficient to generate enzyme variants which hydrolyse (R,S)-mandelonitrile to (R)-mandelic acid with an enantiomeric excess (ee) of 91% or to (S)-mandelic acid with an ee-value of 47%. The conversion of (R,S)-2-phenylpropionitrile by different nitrilase variants resulted in the formation of either (S)- or (R)-2-phenylpropionic acid with ee-values up to about 80%. Furthermore, the amounts of amides that are produced from (R,S)-mandelonitrile and (R,S)-2-phenylpropionitrile could be changed by single point mutations between 2%–94% and <0.2%–73%, respectively. The present study attempted to collect and compare the results obtained during our previous work, and to obtain additional general information about the relationship of the amide forming capacity of nitrilases and the enantiomeric composition of the products.

Temperature-dependent changes to host-parasite interactions alter the thermal performance of a bacterial host.

Thermal performance curves (TPCs) are used to predict changes in species interactions, and hence, range shifts, disease dynamics and community composition, under forecasted climate change. Species interactions might in turn affect TPCs. Here, we investigate how temperature-dependent changes in a microbial host-parasite interaction (the bacterium Pseudomonas fluorescens, and its lytic bacteriophage, SBW[Formula: see text]) changes the host TPC and the ecological and evolutionary mechanisms underlying these changes. The bacteriophage had a narrower thermal tolerance for infection, with their critical thermal maximum ~6 °C lower than those at which the bacteria still had high growth. Consequently, in the presence of phage, the host TPC changed, resulting in a lower maximum growth rate. These changes were not just driven by differences in thermal tolerance, with temperature-dependent costs of evolved resistance also playing a major role: the largest cost of resistance occurred at the temperature at which bacteria grew best in the absence of phage. Our work highlights how ecological and evolutionary mechanisms can alter the effect of a parasite on host thermal performance, even over very short timescales.

Fire Ant Venom Alkaloids Inhibit Biofilm Formation

Biofilm formation on exposed surfaces is a serious issue for the food industry and medical health facilities. There are many proposed strategies to delay, reduce, or even eliminate biofilm formation on surfaces. The present study focuses on the applicability of fire ant venom alkaloids (aka ‘solenopsins’, from Solenopsis invicta) tested on polystyrene and stainless steel surfaces relative to the adhesion and biofilm-formation by the bacterium Pseudomonas fluorescens. Conditioning with solenopsins demonstrates significant reduction of bacterial adhesion. Inhibition rates were 62.7% on polystyrene and 59.0% on stainless steel surfaces. In addition, solenopsins drastically reduced cell populations already growing on conditioned surfaces. Contrary to assumptions by previous authors, solenopsins tested negative for amphipathic properties, thus understanding the mechanisms behind the observed effects still relies on further investigation.

Determination of minimum inhibitory concentration and half maximal inhibitory concentration of antibiotics and their degradation products to assess the eco-toxicological potential

Numerous studies have been reported on today’s ubiquitous presence of antibiotics in surface waters causing the emergence of multidrug-resistant bacteria. Advanced water treatment procedures aim at the complete prevention and elimination of antibiotics from entering natural water bodies. In this study, photoinduced degradation processes using UVC-irradiation were applied toward selected fluoroquinolone, tetracycline, macrolide, and sulfonamide antibiotics. Photodegradation products were elucidated using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). Rate constants and quantum efficiencies were determined for the active substances and their photo-transformation products. As a measure of ecotoxicity, minimum inhibitory and half-maximal inhibitory concentrations were determined against the Gram-negative bacterium Pseudomonas fluorescens and the Gram-positive Bacillus subtilis in a standard assay format. These values were further recorded as a function of UV exposure time. The irradiation time-dependent increase of minimum inhibitory concentration (MIC) values agreed well with the kinetic models. After 10 min of irradiation, the compound solutions ceased to inhibit bacterial cell growth, indicating removal of the pharmaceutical activity. These findings were found in agreement with quantitative structure–activity relationship analysis. The combination of microbiological activity testing, molecular structure analysis, chemical kinetic investigation, and quantitative structure–activity relationship models proved to be able to predict irradiation times and evaluate potential ecotoxicological hazard of the irradiated drugs.

Bacteria solubilization of shenmu lignite: influence of surfactants and characterization of the biosolubilization products

ABSTRACT The biosolubilization experiments of Shenmu lignite were carried out by four kinds of bacteria, Ochrobactrum cytisi, Novospingobium naphthalenivorans, Alcaligenes faecalis, and Pseudomonas fluorescens. The optimal conditions of coal biosolubilization were obtained by combining single-factor and orthogonal experiments, showing the coal biosolubilization rate of dominant bacterium Pseudomonas fluorescens is 53.6%. The effects of four surfactants which were SDS, LAS, Tween 80, and Triton X-100 on coal biosolubilization were studied, it is obvious for Pseudomonas fluorescens the biosolubilization rate can reach to 61.9% and the biosolubilization time has been shortened in the presence of Triton X-100. Adsorption model of surfactants on coal surface was deduced by measuring the contact angle between oxidized coal surface and surfactants solutions, and zeta potential of mixture solution of coal and surfactant. The enhancement of the hydrophilicity of the oxidized coal surface is helpful to improve the coal biosolubilization. The unsaturated compounds with low molecular weight (89–604) such as ethers, esters and the like existed in black liquid products. The carbonyl and nitryl groups in oxidized coal increased after the coal pretreated by nitric acid, while decreased in residual coal. The residual coal surface became loose and rough with corrosive morphology after biosolubilization.

Endophytic Pseudomonas fluorescens induced sesquiterpenoid accumulation mediated by gibberellic acid and jasmonic acid in Atractylodes macrocephala Koidz plantlets

Atractylodes macrocephala is a traditional Chinese medicinal plant, its main bioactive components were sesquiterpenoids. An endophytic bacterium Pseudomonas fluorescens ALEB7B isolated from the stem of Atractylodes lancea was found to enhance sesquiterpenoid content by 20–74% in the root of A. macrocephala. However, the mechanism of endophytes inducing secondary metabolites biosynthesis in A. macrocephala is unknown. To elucidate the signaling mechanism and molecular basis of ALEB7B induced-sesquiterpenoid accumulation in A. macrocephala, the signaling involved in this process and the activity of key enzymes 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) were detected. The results showed that gibberellic acid (GA) and jasmonic acid (JA) signals cooperatively contributed to the bacterium inducing sesquiterpenoid accumulation. The bacterium, JA and GA signals can increase the activities of both HMGR and DXR. By comparing the results of endophytic bacterium interactions with A. lancea, we found that ALEB7B can enhance the sesquiterpenoids content in A. macrocephala and A. lancea; however, the responsive signaling pathways induced by ALEB7B in A. macrocephala and A. lancea were different. In conclusion, endophytes may enhance sesquiterpenoid production in A. macrocephala plants by modulating the GA and JA signaling pathways. This study found that Pseudomonas fluorescens ALEB7B can efficiently promote sesquiterpenoid accumulation in Atractylodes macrocephala root by inducing GA and JA bursting and increasing activities of HMGR and DXR.

Bacteriophage‐based cocktail modulates selected immunological parameters and post‐challenge survival of rainbow trout (Oncorhynchus mykiss)

Recently, a rapid increase in the resistance of pathogenic bacteria to antibiotics and chemotherapeutics admitted for use in aquaculture has been observed. This happens especially often in intensive breeding. The use of drugs in closed circuits is problematic because it can damage biological filters. Therefore, in recent years, there has been a growing interest in natural methods of combating pathogens. These include bacteriophages. The aim of the study was to determine the safety of the new BAFADOR® bacteriophage-based preparation, its effect on selected immunological parameters and the effectiveness of prophylactic and therapeutic use after experimental infections with pathogenic bacteria Aeromonas hydrophila and Pseudomonas fluorescens. The use of BAFADOR® increased the activity of lysozyme, total protein level and immunoglobulin level. The level of ceruloplasmin in the rainbow trout serum remained unchanged regardless of the route of administration of the preparation. Potential killing activity and metabolic activity of spleen phagocytes and proliferation of pronephros lymphocytes were higher compared to the control group. Both therapeutic and prophylactic application of the preparation after mixed experimental infection of A.hydrophila and P.fluorescens limited the mortality of rainbow trout.

IDENTIFICATION OF CONTAMINATION OF SALMON MEAT BY DNA FROM BACTERIA OF THE PSEUDOMONAS FLUORESCENS GROUP IN THE IMPLEMENTATION OF THE DNA BARCODING OF PRODUCTS OF JAPANESE CUISINE

DNA barcoding as a tool for genetic certification and identification of taxonomic membership of organisms has recently become very popular. We have applied DNA barcoding method for taxonomic identification of fish ingredients (salmon, tuna, flying fish roe, shark) in product of Japanese cuisine. We have analyzed 27 samples from 6 retail outlets and 3 restaurants of the city of Voronezh. It was found that for products designated as containing “salmon” in all selected outlets were used as a fillet of Atlantic salmon (Salmo salar), which is considerably less valuable than Pacific salmon. Results of DNA barcoding showed that one of the samples of «salmon» was in fact, yellowfin tuna, whereas one sample of “tuna” was a fillet of Atlantic salmon. However in general, the “salmon” and “tuna” samples were substituted infrequently. Analysis of “flying fish roe” samples revealed that only one sample from 6 was really Fourwing flyingfish (Hirundichthys affinis). The remaining samples were identified as DNA of capelin (Mallotus villosus). By analyzing the “double” sequences in 2 samples, we have found that two samples of «flying fish roe» were mixtures of Fourwing flyingfish roe and capelin roe. A sample labeled “shark” was identified as Mozambique tilapia. This species is a freshwater fish. Bacterium Pseudomonas fluorescens was identified in one of the “salmon” samples. Analysis of “double” sequences of “salmon” samples revealed presence of P. fluorescens DNA. Analysis of salmon meat with the use of species-specific primers for this bacterium revealed contamination of all samples by P. fluorescens. This bacterium causes the disease in salmon and can be harmful to patients with compromised immune systems. Primers used for DNA barcoding were shown to have high homology to DNA of bacterial group P. fluorescens.

Ribosome Provisioning Activates a Bistable Switch Coupled to Fast Exit from Stationary Phase.

Observations of bacteria at the single-cell level have revealed many instances of phenotypic heterogeneity within otherwise clonal populations, but the selective causes, molecular bases, and broader ecological relevance remain poorly understood. In an earlier experiment in which the bacterium Pseudomonas fluorescens SBW25 was propagated under a selective regime that mimicked the host immune response, a genotype evolved that stochastically switched between capsulation states. The genetic cause was a mutation in carB that decreased the pyrimidine pool (and growth rate), lowering the activation threshold of a preexisting but hitherto unrecognized phenotypic switch. Genetic components surrounding bifurcation of UTP flux toward DNA/RNA or UDP-glucose (a precursor of colanic acid forming the capsules) were implicated as key components. Extending these molecular analyses—and based on a combination of genetics, transcriptomics, biochemistry, and mathematical modeling—we show that pyrimidine limitation triggers an increase in ribosome biosynthesis and that switching is caused by competition between ribosomes and CsrA/RsmA proteins for the mRNA transcript of a positively autoregulated activator of colanic acid biosynthesis. We additionally show that in the ancestral bacterium the switch is part of a program that determines stochastic entry into a semiquiescent capsulated state, ensures that such cells are provisioned with excess ribosomes, and enables provisioned cells to exit rapidly from stationary phase under permissive conditions.

Promotion of the root development and Zn uptake of Sedum alfredii was achieved by an endophytic bacterium Sasm05

Endophyte-assisted phytoremediation has gained increasing attention. However, the interacting mechanisms of endophytes and metal hyperaccumulators are still not clear. An endophytic bacterium Pseudomonas fluorescens Sasm05 inoculation promoted Sedum alfredii Hance rooting and root development, in which the specific root length (SRL) and average number of root tips (ART) increased to 2.09- and 3.35-fold, respectively. Sasm05 inoculation promoted plant growth, increased the chlorophyll content, and elevated Zn uptake of plant at excess Zn supply. At 200 μM Zn treatment level, Sasm05 inoculation increased plant biomass and the chlorophyll content by more than 40%, and root Zn content by 40%. Furthermore, Sasm05 inoculation upregulated the expression of the Zn transporter SaIRT1 to 3.43-fold in the roots, while another transporter SaNramp1 expression was increased to 38.66-fold in the roots and 7.53-fold in the shoots. Time course study showed the best effects of Sasm05 on plant biomass and the chlorophyll content were detected at 30 d, while for Zn content at 3 d. These results firstly provided molecular evidences of endophytic bacteria in facilitating host plant Zn uptake, which will absolutely benefit the understanding of interacting mechanisms between hyperaccumulators and their endophytes.