BioTechniquesVol. 24, No. 5 BenchmarksOpen AccessFusion PCR, a One-Step Variant of the “Megaprimer” Method of MutagenesisChristiaan KarremanChristiaan Karreman*Address correspondence to Dr. Christiaan Karreman, GSF–National Research Center for Environment and Health, Institute of Clinical Molecular Biology and Tumour Genetics, Marchioninistr. 25, 81377, Munich, Germany. Internet: E-mail Address: karreman@gsf.deGSF–National Research Center for Environment and Health, Munich, GermanySearch for more papers by this authorPublished Online:15 Aug 2018https://doi.org/10.2144/98245bm08AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByThe horizontal transfer of Pseudomonas aeruginosa PA14 ICE PAPI-1 is controlled by a transcriptional triad between TprA, NdpA2 and MvaT13 October 2021 | Nucleic Acids Research, Vol. 49, No. 19Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation21 July 2021 | Biomolecules, Vol. 11, No. 8Streptococcus pneumoniae Senses a Human-like Sialic Acid Profile via the Response Regulator CiaRCell Host & Microbe, Vol. 20, No. 3Development of a simple and rapid protocol for the production of customized intertypic recombinant poliovirusesJournal of Virological Methods, Vol. 186, No. 1-2Construction of Tandem Repeats of DNA Fragments by a Polymerase Chain Reaction-Based MethodDNA and Cell Biology, Vol. 31, No. 4Identification of antigen-specific residues on E2 glycoprotein of classical swine fever virusVirus Research, Vol. 152, No. 1-2A lentiviral vector encoding two fluorescent proteins enables imaging of adenoviral infection via adenovirus-encoded miRNAs in single living cells16 September 2009 | The Journal of Biochemistry, Vol. 147, No. 1Rapid and Efficient Gene Splicing Using Megaprimer-Based Protocol24 June 2008 | Molecular Biotechnology, Vol. 40, No. 3Identification of the Acyltransferase that Octanoylates Ghrelin, an Appetite-Stimulating Peptide HormoneCell, Vol. 132, No. 3Modulation of Ca2+ entry and plasma membrane potential by human TRPM4b15 December 2006 | FEBS Journal, Vol. 274, No. 3Leishmania infantum LeIF protein is an ATP-dependent RNA helicase and an eIF4A-like factor that inhibits translation in yeastFEBS Journal, Vol. 273, No. 22Structural cooperativity in spectrin type repeats motifs of dystrophinBiochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1764, No. 5Pyruvate Oxidase Is a Determinant of Avery's Rough MorphologyJournal of Bacteriology, Vol. 186, No. 24Domain Swapping Localizes the Structural Determinants of Regioselectivity in Membrane-bound Fatty Acid Desaturases of Caenorhabditis elegansJournal of Biological Chemistry, Vol. 279, No. 38Large-Scale Exogenous Protein Production in Higher Animal CellsCystathionine?-synthase deficiency in Georgia (USA): Correlation of clinical and biochemical phenotype with genotype17 November 2003 | Human Mutation, Vol. 22, No. 6PCR-Generated Truncated Positive Control for PCR Screening of Embryonic Stem Cell ClonesDirk Gründemann, Petra Censarek & Edgar Schömig24 September 2018 | BioTechniques, Vol. 34, No. 6A rapid and convenient variant of fusion-PCR to construct chimeric flavivirusesJournal of Virological Methods, Vol. 108, No. 1The Q MotifMolecular Cell, Vol. 11, No. 1Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of LipaseApplied and Environmental Microbiology, Vol. 68, No. 12Marker-fusion PCR for one-step mutagenesis of essential genes in yeast1 January 2002 | Yeast, Vol. 19, No. 2Nuclear Localization of the Saccharomyces cerevisiae HMG Protein NHP6A Occurs by a Ran-Independent Nonclassical Pathway21 December 2001 | Traffic, Vol. 2, No. 7Genetic analysis of the strong gyrase site (SGS) of bacteriophage Mu: localization of determinants required for promoting Mu replicationMolecular Microbiology, Vol. 37, No. 4Stable High-Level Expression of Heterologous Genes In Vitro and In Vivo by Noncytopathic DNA-Based Kunjin Virus Replicon VectorsJournal of Virology, Vol. 74, No. 9[9] Use of fusions to thymidine kinaseStability and peptide binding specificity of Btk SH2 domain: Molecular basis for X-linked agammaglobulinemiaProtein Science, Vol. 9, No. 12Site-Directed Mutagenesis by Fusion of Contiguous DNA FragmentsSergio Carmona, Marc Passman, Michael Kew & Patrick Arbuthnot22 August 2018 | BioTechniques, Vol. 26, No. 3A new set of positive/negative selectable markers for mammalian cellsGene, Vol. 218, No. 1-2 Vol. 24, No. 5 Follow us on social media for the latest updates Metrics Downloaded 414 times History Published online 15 August 2018 Published in print May 1998 Information© 2018 Author(s)PDF download