Bacteriological Water Quality Research Articles

Physicochemical, Bacteriological and Parasitological Quality of Water Used to Wash Vegetables in Dschang, West Cameroon: Health Risk Assessment

The quality of water used for the cleaning fresh vegetables sold in Cameroonian cities is of little concern. The objective of this study was to determine the health risk associated with the water use for washing fruits and vegetables in the city of Dschang, in order to help develop strategies for the control waterborne diseases in Cameroon in general and in Dschang in particular. After a survey of 75 vegetable vendors in the Dschang market, 40 samples of washing water, 20 per season, were collected randomly throughout the main market in the city of Dschang. Physicochemical, bacteriological and parasitological analyses were carried out according to standard methods. Regardless of the season, bacteria indicative of faecal contamination were detected in the water used for washing fresh vegetables, with levels ranging from 0 to 8×10⁴ CFU/100ml for <i>Escherichia. coli</i>, from 1.8×10⁴ to 1.8×10⁸ CFU/100ml for faecal coliforms, from 3×10⁴ to 7.2×10⁸ CFU/100ml for <i>Salmonella</i> spp., from 30 to 1.3×10⁷ CFU/100ml for <i>Shigella</i> spp. and 20 to 7.5×10⁵ CFU/100ml for faecal streptococci. <i>Ascaris</i> spp. eggs, <i>Entamoeba</i> spp. cysts and nematode larvae were detected in vegetable washing water, with a prevalence of 40%. Consumers of fresh vegetables washing by these waters, if raw and poorly washed, are at risk of bloody diarrhoea, gastroenteritis, typhoid and paratyphoid fever or inflammation of the colon tissue and severe necrosis. The authorities should introduce food hygiene in the markets as part of the strategies to combat waterborne diseases in this city. This solution is also valid for other cities in Cameroun.

Epidemiological significance of the occurrence and persistence of rotaviruses in water and sewage: a critical review and proposal for routine microbiological monitoring.

Globally, waterborne gastroenteritis attributable to rotaviruses is on the increase due to the rapid increase in population growth, poor socioeconomic conditions, and drastic changes in climatic conditions. The burden of diarrhea is quite alarming in developing nations where the majority of the populations still rely on untreated surface water that is usually polluted for their immediate water needs. Humans and animals of all ages are affected by rotaviruses. In humans, the preponderance of cases occurs in children under 5 years. Global efforts in advancing water/wastewater treatment technologies have not yet realized the objective of complete viral removal from wastewater. Most times, surface waters are impacted heavily by inadequately treated wastewater run-offs thereby exposing people or animals to preventable health risks. The relative stability of rotaviruses in aquatic matrices during wastewater treatment, poor correlation of bacteriological indicators with the presence of rotaviruses, and their infectiousness at a low dose informed the proposal for inclusion in the routine microbiological water screening panel. Environmental monitoring data have been shown to provide early warnings that can complement clinical data used to monitor the impact of current rotavirus vaccination in a community. This review was therefore undertaken to critically appraise rotavirus excretion and emission pathways, and the existence, viability and persistence in the receiving aquatic milieu. The efficiency of the current wastewater treatment modality for rotavirus removal, correlation of the current bacteriological water quality assessment strategy, public health risks and current laboratory methods for an epidemiological study were also discussed.

Multiresistant bacteria: Invisible enemies of freshwater mussels

Freshwater mussels are among the most endangered groups of fauna anywhere in world. The indiscriminate use of antibiotics has led to the emergence of resistant strains. These antibiotic-resistant bacteria play a key role in increasing the risk allied with the use of surface water and in spread of resistance genes. Two endangered freshwater mussel species, Margaritifera margaritifera and Potomida littoralis, were sampled at 4 sampling sites along a 50 km stretch of River Tua. Water samples were taken at same sites. Of the total of 135 isolates, 64.44% (39.26% from water and 25.19% from mussels) were coliform bacteria. Site T1, with the lowest concentration of coliform bacteria, and site T2 were the only ones where M. margaritifera was found. No E. coli isolates were found in this species and the pattern between water and mussels was similar. P. littoralis, which was present at T3/T4 sites, is the one that faces the highest concentration of bacterial toxins, which are found in treated wastewater effluents and around population centers. Sites T3/T4 have the isolates (water and mussels) with the highest resistance pattern, mainly to β-lactams. Water and P. littoralis isolates (T3/T4) showed resistance to penicillins and their combination with clavulanic acid, and to cephalosporins, precisely to a fourth generation of cephalosporin antibiotics. The analysis provides important information on the risk to water systems, as well as the need to investigate possible management measures. It is suggested that future studies on the health status of freshwater bivalves should incorporate measures to indicate bacteriological water quality.

Bacteriological quality of household drinking water in North Gondar Zone, Ethiopia; a community-based cross-sectional study

Ethiopia is one of the developing countries where only 52% of its population have access to safe drinking water. To determine the level of faecal contamination in drinking water, the E. coli has been found to be the most specific indicator. The purpose of this study was to determine household bacteriological drinking water quality and its associated factors in rural North Gondar Zone Ethiopia. A community-based cross-sectional study was conducted in rural parts of North Gondar Zone from April to June, 2016. Water samples from water storage at household level were taken using standardized water sampling techniques to determine the level of faecal contamination. Face-to-face interview using structured questionnaires and checklist were used to identify the associated factors. A total of 736 households participated in this study. The prevalence of positive faecal coliform in the households is found to be 72.6% (95%C.I; 69.4–75.8). In multivariate analysis; occupational status [AOR; 0.35, 95%; CI (0.16,0.77)], income [(AOR);0.45, 95%; CI (0.3, 0.68)], type of solid waste disposal habit [AOR; 0.16, 95%; CI (0.36, 0.66)], source of water [AOR; 0.42, 95%; CI (0.28, 0.65)], shortage of water [AOR; 2.8, 95%; CI (1.88, 4)] were variables identified as predictors for faecal coliform contamination of water in the household. There is high level of faecal contamination of household drinking water in North Gondar Zone. Occupational status, income, solid waste disposal habit, sources of drinking water, and shortage of water were predictor for presence of faecal coliform contamination in household drinking water.

Bacteriological Analysis of Household Water at the Source and Point of Consumption at Karwar, India

Access to safe drinking water is the key to promoting good health. At Karwar, the primary source of drinking water is well, but heavy rain, and deficiencies in underground drainage systems result in well water contamination. However, water handling practices followed at the point of consumption act as a better predictor of water contamination. To assess and compare the bacteriological quality of household water at the source and point of consumption. A prospective cross-sectional study was conducted at Karwar. Households owning private wells were selected by Simple Random Sampling. Semi-structured questionnaires were used to interview the households regarding socioeconomic status (SES), chlorination of well, purification method, and water handling practices. Presumptive coliform count and the differential coliform count were determined. Data thus obtained were analyzed using a Microsoft Excel. c2-test was used to see the association between variables. The present study included 30 households. All the samples taken from the well revealed contamination with E. coli. 80% of samples from the point of consumption revealed coliforms, among which 47% were confirmed with E. coli. RO/RO+UV were found to be relatively better performing than the filtration and boiling methods. Contamination of water at the point of consumption had a significant association with both purification and water retrieval methods. The samples taken from the point of consumption reflect better quality of water sampling than source water. The present study revealed post-collection contamination as major reason for compromising water quality despite improved water cleaning and purification techniques.

Quality Assessment of Underground Water before and afterTreatment: A Case Study of Tobruk City, Libya

The present study was carried out to assess the quality of various wells for drinking purposes and with regard to their current state of repair. Ten wells (W1, W2, W3, W4, W5, W6, W7, W8, W9 and W10) were selected in the study area. This study was designed to determine the physico-chemical and bacteriological quality of wells water before and after water treatment in east Libya, specifically in Tobruk. The bacterial load (E. Coli) of the water samples was determined using standard microbiological methods. Physicochemical properties including pH, total dissolved solids (TDS), electrical conductivity, and Na⁺, K⁺, Ca²⁺, Cl⁻, NaCl concentrations and total alkalinity were determined. The values obtained were compared with the World Health Organization (WHO) standards for drinking water. The results showed that E. Coli bacteria were present in some wells prior to treatment, but were E. Coli-free post-treatment. Furthermore, the physico-chemical parameters of the water samples were generally greater the limits recommended by the WHO with particularly high values seen in well W10, prior to treatment. However, only a few of the bacteriological and physicochemical parameters of the water samples remained above the tolerable limits recommended by the WHO (WHO, 2011) post-treatment. This suggests that regular monitoring and purification of boreholes to ensure good water quality is vital to maintaining the required health standards.

Physicochemical and Bacteriological Water Quality Parameters in Relation to Land-use Practices at a Rural Catchment, Mbinga District, Tanzania

Data on physicochemical water quality parameters and contamination levels is crucial for protection and sustainable utilization of water resources. This study assessed the physicochemical water quality parameters, faecal coliform bacteria and nutrient loads in water at a catchment used for municipal supply in Mbinga District, Tanzania. Samples were seasonally collected from three areas characterized by distinct geographical features, settlement patterns and land-use practices, and analyzed using standard analytical protocols. Data were subjected to paired-sampled t-test and cluster analysis. The parameters varied as follows: pH (4.75–6.83); temperature (16.4–26.2 ºC); electrical conductivity (14.6–121.1 µS/cm); total dissolved solids (7.0–23.2 mg/L); total suspended solids (BDL–35.5 mg/L); dissolved oxygen (8.6–23.8 mg/L); turbidity (0.1–68.1 NTU); faecal coliform (&lt;1–76 FC/100 mL); nitrite (BDL–0.09 mg/L); nitrate and sulphate were not detected. Spatial variations were associated with land-use disparities. Comparison to risk-based regulatory thresholds for drinking water revealed the levels to be within the acceptable limits, with a few exceptions. Observed seasonal trends indicated an overall decrease in water quality during the wet season. Generally, results showed that water quality at the catchments satisfies requirements for domestic uses. However, the potential contamination risks from human activities call for regular monitoring.&#x0D; Keywords: Physicochemical parameters; faecal contamination; water quality; surface water; Mbinga District

Rapid and improved identification of drinking water bacteria using the Drinking Water Library, a dedicated MALDI-TOF MS database

According to the European Directives (UE) 2020/2184 and 2009/54/EC, which establishes the sanitary criteria for water intended for human consumption in Europe, water suitable for human consumption must be free of the bacterial indicators Escherichia coli, Clostridium perfringens and Enterococcus spp. Drinking water is also monitored for heterotrophic bacteria, which are not a human health risk, but can serve as an index of bacteriological water quality. Therefore, a rapid, accurate, and cost-effective method for the identification of these colonies would improve our understanding of the culturable bacteria of drinking water and facilitate the task of water management by treatment facilities. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is potentially such a method, although most of the currently available mass spectral libraries have been developed in a clinical setting and have limited environmental applicability. In this work, a MALDI-TOF MS drinking water library (DWL) was defined and developed by targeting bacteria present in water intended for human consumption. This database, made up of 319 different bacterial strains, can contribute to the routine microbiological control of either treated drinking water or mineral bottled water carried out by water treatment and distribution operators, offering a faster identification rate compared to a clinical sample-based library. The DWL, made up of 96 bacterial genera, 44 of which are not represented in the MALDI-TOF MS bacterial Bruker Daltonics (BDAL) database, was found to significantly improve the identification of bacteria present in drinking water.

Bacteriological Quality of Potable Water Consumed in Cape Coast and Takoradi Metropolis, Ghana

Background: Bacterial contamination of potable water remains a global canker and has been reported to result in deaths from gastrointestinal infections. Treatment of gastrointestinal infections is becoming difficult due to antimicrobial resistance. This study sought to assess the bacteriological quality of potable water consumed in Cape Coast and Takoradi Metropolis of Ghana.&#x0D; Methodology: Eighty-seven (87) samples of potable water were collected from various vicinities of Cape Coast (43) and Takoradi (44). The samples were analyzed for their bacterial loads using various laboratory bacteriological procedures and the resulting colonies were subjected to standard identification techniques. Antimicrobial Susceptibility Testing (AST) was carried out to determine the susceptibility patterns of the various isolates.&#x0D; Results: A total of 220 bacterial isolates were identified comprising 18 species, with Bacillus cereus. (13.6%), Staphylococcus aureus (8.5%), and Klebsiella sp. (10%), being predominant and Pseudomonas sp. (2.3%), Streptococcus sp. (1.8%), and Serratia sp. (0.5%) being less frequent. Antimicrobial Sensitivity Testing (AST) revealed multiple antimicrobial-resistant bacteria including, Escherichia coli, Pseudomonas sp., and Klebsiella sp. The average HPC and TCC of the various samples ranged from 0.20 - 1.94 x 108 CFU/ml and 0.00 - 2.39 x 108 CFU/ml respectively.&#x0D; Conclusion: Some potable water in Cape Coast and Takoradi metropolis including most sachet water sold on the streets were found to be highly contaminated with bacteria.

Physicochemical and Bacteriological Quality of Water from Storage Tanks in a Tertiary Institution in Rivers State, Nigeria

Water quality analysis is essential in assessing the suitability of different water sources used for various purposes, including industrial and domestic uses. This study was therefore aimed at determining the physicochemical and bacteriological quality of water samples from different storage facilities in a tertiary institution in Port Harcourt, Rivers State, Nigeria. The study involved eighty (80) water samples obtained from water storage tanks situated at 16 locations within the premises of the institution, to ascertain the physicochemical property, presence and population of different bacterial groups influencing the quality of these water sources. In-situ and ex-situ physicochemical analyses as well as bacteriological investigations were carried out on all samples, using standard laboratory procedures. Results of physicochemical analysis showed that the pH ranged from 4.15±0.14 to 7.16±0.08; conductivity, from 50.55±0.49 (µs/cm) to 364.00±2.83; salinity, from 0.02±0 (ppt) to 0.18±0; temperature, from 27oC to 28oC; Chloride, from 1.03±0.06 (mg/l) to 10.80±0.79; total alkalinity, from 4.00±0 (mg/l) to 11.00±1.41; Dissolved oxygen from 3.04±.020 to 7.36±0.08 (Mg/l) and BOD ranged from 0.81±0 to 4.23±0.09 (Mg/l). Results for bacterial population showed total heterotrophic bacteria ranging from 1.03±1.19 x 103 CFU/ml in water from reservoir tanks at the Faculty of Engineering, to 5.89±2.59 x 103 CFU/ml at Road A Block B; total coliform count ranged from 0 CFU/ml in most samples, to 10.00±2.36 x 102 CFU/ml in Block B reservoir tanks. Water reservoirs in clinic area recorded the highest Salmonella/Shigella counts (1.00±0.23 x 101 CFU/ml) with other stations having zero counts. Hall F (Hostel Ext) on the other hand had the highest counts for Vibrio (2.20±3.01 x 101 CFU/ml). The phenotypic characterization identified Citrobacter spp. as the most occurring (27.27%) bacterial isolate in the study, followed by Alcaligenes faecalis and Klebsiella spp. (18.18% each). Enterobacter spp., Edwardsiella spp., Erwinia psidii, Acinetobacter spp., Pseudomonas spp., Providentia spp. and Salmonella spp. all appeared as the least occurring, having a percentage of 4.55%, each. Tatumella spp. on the other hand had a percentage occurrence of 9.09%. This study has buttressed the need for increased water hygiene of reservoir tanks as well as water sources in these locations.

Bacteriological Biodiversity and its Relationship with the Quality of Water in Panvel Lakes (Vishrale, Krishnale and Dewale Lake) at Raigad (Maharashtra), India

This research is carried out to study the bacteriological biodiversity and quality of water during premonsoon, monsoon and post-monsoon in Panvel lakes, Maharashtra, India. Most Probable Number (MPN) count was carried out by using Mac Conkey’s broth in order to classify the lakes into different categories. Pathogenic bacteria colonies were isolated from water samples collected from different lakes, by spread plate method. After incubation, different colonies from incubated plates were picked; purified by repeated sub-culturing before being examined for gram reaction, microscopically bacteria were identified into positive or negative, cocci or bacilli, rod or spiral. Biochemical analysis was carried out using different tests. The results were analyzed using Bergey’s manual of systematic bacteriology. Many different types of bacteria were isolated from water samples that helped in classifying the lakes and knowing the bacterial loads of each lake.

Effect of storage container on the bacteriological quality of water from different sources

Effect of storage container on the bacteriological quality of water from different sources

Detection of Bacteriological Contaminants in Hand-Pump Fitted Borehole Water from a Residential Suburb in Ringim Local Government, Jigawa State

Water from borehole source can be contaminated due to indiscriminate waste disposal. This pilot study was conducted to assess the bacteriological quality of water from five (5) hand-pump-fitted borehole sources present in Sabon Gari quarters in Ringim Local Government Area of Jigawa State. Presumptive, confirmed and completed tests were employed to determine total and faecal coliforms from the water samples using most probable number (MPN) technique. Bacterial isolates were identified by standard microbiologicalmethods. Results obtained indicate that all the water samples across all the dilution strengths tested recorded substantial growth of total coliforms at 37.3°C while faecal coliforms were observed at 44°C after incubation for 48 h. Using the MPN table, the five water samples assayed recorded 50 MPN/100mL, 14 MPN/100mL, 3 MPN/100mL, 5 MPN/100mL and 1 MPN/100mL. Results were also positive for gas and acid production in all samples collected characteristic of presence of E. coli. The results obtained in this study show bacterial contamination of the water from the boreholes which may constitute a public health risk. It is therefore recommended that the water pumped from these boreholes should be subjected to adequate treatment to meet World Health Organization (WHO) required standard for potable water meant for drinking.&#x0D; Keywords: Boreholes, hand pump, most probable number, total coliforms and faecal coliforms.

Bacteriological Quality of Water Used by Street Food Vendors in Jabalpur City of Central India

Bacteriological Quality of Water Used by Street Food Vendors in Jabalpur City of Central India

Microbiological quality and determination of pesticide residues in the lettuce produced in Bahía Blanca's horticultural belt, Argentina

The aims of this research were 1) to find out the possible microbiological contamination and residues of pesticides (chlorpyrifos and cypermethrin) in lettuce grown in Bahía Blanca’s horticultural belt, and bacteriological quality of waters used for irrigation and post-harvest washing, 2) to investigate and compare the internalization capacity of a strain of Escherichia coli isolated from irrigation water with another hospital uropathogenic strain. Some strains of bacteria can internalize into the plant tissue. This capability presents food safety threats as they cannot be removed with standard sanitation practices. To determine bacteriological quality of lettuce, E. coli, Salmonella spp and E. coli O157: H7/NM (n = 10) were investigated. Bacteriological quality of water was determined by quantification of fecal coliforms/100 mL (n = 20). Pesticides were determined by using an improved and validated QuEChERS method combined with GC-MSD. Irrigation water in 19/20 samples did not present health risk. Bacteriological quality of lettuce met demands in 9/10 samples. Pesticides were detected in half of the samples, values exceeding the FAO recommendations. An effort is strongly recommended to implement both, best phytosanitary practices and monitoring programmes. Uropathogenic strain was able to internalize into plant tissue, while the nonpathogenic one was not.

Bacteriological and Physicochemical Quality of Drinking Water in Adis Kidame Town, Northwest Ethiopia.

Drinking water pollution and the resulting waterborne diseases have been among the major public health burden in low-income countries such as Ethiopia. A high prevalence of waterborne diseases, up to 65%, has been reported in Adis Kidame Town (Ethiopia). Moreover, there have been poor waste disposal practices in this town. Thus, this study aimed to assess bacteriological and physicochemical drinking water quality in Adis Kidame Town to get insight into any potential health risks due to waterborne diseases. A total of 90 water samples were analyzed for enumeration of coliform bacteria and basic physicochemical parameters. In this study, total and faecal coliform counts (CFU/100 ml) ranged from 0 to 23 and 0 to 18, respectively. In all cases, 89 and 77% of the water samples in terms of total and faecal coliforms, respectively, did not comply with WHO guidelines. The highest level of coliforms was found in drinking water samples from households' containers and taps, where 100% and 90% of samples were tested positive for total and faecal coliforms, respectively. Among the physicochemical parameters recorded, only temperature and residual chlorine did not comply with WHO guidelines. Temperature values in all samples ranged from 20 to 28°C, while the values of residual chlorine were below the recommended range in the 56.7% of water samples from the reservoir and almost in all (96.7%) water samples from the taps and households' containers. High coliform count in the water system demands proper chlorination, regular inspection of the distribution line, and good hygienic practices to improve the microbiological quality of drinking water in Adis Kidame Town.

Bacteriological and physicochemical quality of drinking water in Kobo town, Northern Ethiopia

Abstract Despite drinking water supply in Kobo town is from a borehole through pipes, a high incidence of waterborne diseases are frequently reported. Hence, this study aimed to assess the bacteriological and physicochemical drinking water quality in Kobo town. One hundred and twenty water samples were collected from four sampling sites (the source, reservoir, taps, and households' containers) from February to April 2020. Total and fecal coliforms were counted from the water samples using membrane filtration while selected physicochemical parameters were determined using standard methods. The mean counts of total and fecal coliforms ranged from 3.9 to 22.9 and 1 to 13.6 CFU/100 mL, respectively. Hence, all water samples did not satisfy the WHO guidelines and national standards. There were statistically significant differences in the coliform counts between the different sampling sites, and the counts were significantly higher in the taps and households' containers compared to the counts in the source and reservoir (p &amp;lt; 0.05). All physicochemical parameters, except for temperature, were within the recommended acceptable limits. High coliform count in the water system demands proper maintenance of the distribution line and good hygiene practices at household level to improve the microbiological quality of drinking water in Kobo town.

Bacteriological and Physicochemical Quality of Drinking Water in Wegeda Town, Northwest Ethiopia.

Waterborne diseases continue to challenge communities in low-income countries like Ethiopia. Clinical information in Wegeda town showed that the prevalence of waterborne diseases was 58%. This study aimed to evaluate bacteriological and physicochemical drinking water quality in Wegeda town. This study will add valuable scientific data for future intervention. Water samples from protected and unprotected springs, hand-dug well, taps, and households' containers were collected from November 2018 to June 2019 for bacteriological and physicochemical analyses. Besides, information about the potential risk factors was collected using a structured questionnaire. A total of 120 water samples were collected and analyzed for total and fecal coliform counts using the multiple tube fermentation method (MPN). The presence of Escherichia coli was also checked from fecal coliform positive samples collected from households' containers. Selected physicochemical parameters were also determined using the standard methods. In all cases, the median values of total and fecal coliform counts ranged from 5 to 27 and 2 to 13 MPN/100 ml, respectively. Accordingly, all of the drinking water samples did not comply with the standards. Coliforms were significantly higher in the households' containers than in the sources (p < 0.05) and also significantly varied by water sources. The highest and lowest coliform counts were recorded in unprotected spring and taps, respectively. Besides, 18.33% of water samples collected from households' containers were tested positive for E. coli. Regarding physicochemical parameters, most values were within the acceptable limit values recommended by the WHO. However, water samples from unprotected spring and hand-dug well did not satisfy the turbidity limit value set by the WHO. Drinking water systems in Wegeda town were likely contaminated with pathogenic bacteria likely due to poor protection and sanitation practices. Providing the community with potable water, toilets, domestic and animal waste disposal systems, and intensive health education and sanitation practices for the community are highly recommended.

Assessment of water quality of River Ganga during COVID-19 lockdown

We have done a qualitative and quantitative analysis of Ganga River water in two areas namely Palta and Diamond Harbour, in the state of West Bengal, India. Anthropogenic activity is very high in these regions. Restriction of human activity near river basins due to the prolonged COVID-19 lockdown has brought remarkable changes in the environment. A comparison of the pre-lockdown period and the lockdown period was done. The study covered the years from March 2019 to May 2020. Results demonstrate improvement in surface water quality of River Ganga, during the lock-down period as there was less anthropogenic activity. The water quality test revealed that tur-bidity has reduced to <94% during the lockdown. River Ganga was one of the polluted rivers, unfit for a bath but physicochemical properties like turbidity, total suspended solids, and total dissolved solids have improved enormously during the lockdown. The chemical oxygen demand, biochemical oxygen demand has changed from 12 and 3 mg/L to <6 and 1.2 mg/L, respectively. Consecutively, dissolved oxygen level has increased from 6 to 12 mg/L. Low total coliform and fecal coliform counts indicated improvement in the bacteriological quality of water. The results of the present investigation establish a significant improvement in water quality. © 2021 Desalination Publications. All rights reserved.

Assessment of health risks related to groundwater consumption in Al-Hoceima region

Water is a precious natural resource for life, and more vulnerable to different contaminations; it has become less and less drinkable because it is polluted and can carry pathogenic germs that cause water diseases. The population of the Al-Hoceima region is supplied with drinking water from the network of the National Drinking Water Board, which is not widespread in rural areas where there is the frequent use of communal water points (spring or well). Health concerns remain related to the consumption of untreated well water, in the absence of a department responsible for the management of well water (development, equipment, treatment, protection, and quality control). To preserve the health of the population from any source of contamination carried by water, the Ministry of Health, carries out, through the provincial hygiene services, the control and monitoring of the physical-chemical and bacteriological quality of water in the framework of the fight against water diseases. The objective of this work was to determine the state of groundwater quality in the Al-Hoceima region, based on the study of physicochemical and bacteriological parameters. A control of 41 collective water points was carried out, including 30 wells and 11 springs. The collected samples were analyzed to measure some physical parameters: electrical conductivity, pH, temperature, and dissolved oxygen, possibly looking for indicator germs of fecal contamination, namely coliform bacteria, Escherichia coli, and intestinal enterococci. The results revealed fecal contamination of all collective water points by coliform bacteria and Escherichia coli. Appropriate measures must be taken for the disinfection of these waters before their use.