Bacteriological Profile Research Articles

Clinical Profile, Bacteriological Profile, and Outcomes of Patients with Spontaneous Bacterial Peritonitis with Preexisting Chronic Liver Disease

Clinical Profile, Bacteriological Profile, and Outcomes of Patients with Spontaneous Bacterial Peritonitis with Preexisting Chronic Liver Disease

Bacteriological profile and their antimicrobial susceptibility pattern among clinical suspected adult septicemia admitted patients: A study from tertiary care and teaching hospital

Objectives: The objective of this study is to determine the bacteriological profile and their antimicrobial susceptibility pattern among clinically suspected adult septicemia admitted patients and to study the correlation between the clinical presentation and inflammatory biomarkers (C-reactive protein [CRP] and Procalcitonin [PCT]) in the final outcomes of nosocomial sepsis in adult septicemia patients. Material and Methods: This is a descriptive cross-sectional study of 1-year duration from November 2022 to November 2023. The study has been approved from the Institutional Ethical Committee. Adult patients with a clinical suspected diagnosis of sepsis were admitted to the medicine ward of the tertiary hospital. Blood samples of adult sepsis suspected patients samples were taken for as a part of septicemia screening. Blood culture and sensitivity were performed according to standard guidelines. The sepsis inflammatory prognostic markers CRP and PCT test were evaluated according to manufactures instruction. Results: Out of 300 clinically suspected adult septicemia patients, 93 (31%) patients were isolated culture-positive sepsis while 207 (69%) patients were culture-negative sepsis. Gram-positive organisms were more (69%) as compared to Gram-negative organisms (31%). Staphylococcus aureus was predominant among Gram-positive organisms while Acinetobacter baumanii complex (9%) followed by Enterobacter spp. (8%) isolated in Gram-negative bacilli. Culture-positive sepsis patients have CRP level ≥3.2 mg/dL (80%) and the majority (80%) of these patients have reactive PCT. Conclusions: Successful treatment of bloodstream infection depends on early diagnosis and appropriate use of antimicrobial agents. Rapid identification results and antimicrobial susceptibility tests are essential for guiding clinicians in the selection of the most appropriate treatment for patients with bloodstream infections.

Bacteriological and mycological profile of necrotizing otitis externa in diabetics

Bacteriological and mycological profile of necrotizing otitis externa in diabetics

Aerobic Bacteriological Profile of Surgical Site Infections and their Antibiogram

Aerobic Bacteriological Profile of Surgical Site Infections and their Antibiogram

BACTERIOLOGICAL ANALYSIS OF URINARY TRACT INFECTIONS AT HYDERABAD, SINDH

Urinary Tract Infections (UTI) is a condition in which micro-organisms multiply within the tract to establish infection. The aim of this study was to assess bacteriological profiling of UTI in Hyderabad, Pakistan. Isolation and identification of bacterial uropathogens were carried out using standard microbiological methods such as microscopic analysis, cultural characteristics, and biochemical testing. A total of 320 urine samples were investigated for bacteriological analysis. Among them, 116 (36.26%) urine samples yielded bacterial growth, which included 74 (23.12%) female and 42 (13.13%) male patients. Bacterial distribution revealed that the most frequent isolate was Escherichia. coli which accounted 60.34% (n=70) of the isolates followed by Klebsiella pneumoniae 13.79% (n=16), Pseudomonas aeruginosa 7.76% (n=9), Enterococcus spp. 7.76% (n=9), Streptococcus spp. 4.31% (n=5), Staphylococcus aureus 3.4% (n=4), Proteus mirabilis 2.59% (n=3). It is concluded that the, bacteriological profiling of urine samples revealed seven different uropathogens circulating in Hyderabad with high prevalence of E. coli.

Bacteriological profile and antimicrobial susceptibility patterns of organisms responsible for blood stream infections in intensive care unit patients

Bacteriological profile and antimicrobial susceptibility patterns of organisms responsible for blood stream infections in intensive care unit patients

Efficacy of single-dose versus triple-doses perioperative antimicrobial prophylaxis in patients undergoing laparoscopic cholecystectomy

Introduction: Although debatable, prophylactic antibiotics may be required for patients undergoing cholecystectomy for symptomatic gallstone disease with certain associated risk factors. Our study aimed to perform a non-randomised prospective comparative trial evaluating a single-dose pre-operative antibiotic versus a triple-dose prophylactic post-operative antibiotic in patients with symptomatic uncomplicated cholelithiasis undergoing laparoscopic cholecystectomy. Methodology: This study was a non-randomised prospective comparative study in a single institution. Two hundred patients were enrolled and allocated to two groups. Group A patients were given a single dose of pre-operative prophylactic antibiotic, and Group B patients received a triple dose of prophylactic antibiotic therapy. We also studied the bacteriological profile of bile in all the patients using bile culture and antibiotic sensitivity, and we analysed their association with post-operative surgical site infection (SSI). Results: All the patients were followed up on post-operative days 7 and 30. On post-operative day 7, 2% of patients in Group A and 1% of patients in Group B were found to have SSI, and no statistically significant difference in SSI between the two groups was noted (P = 0.558). On post-operative day 30, no patient was found to have a SSI in either of the two groups. Moreover, post-operative bile culture was obtained in all the cases, and 6% of patients had a positive bile culture. However, none of them developed SSIs on post-operative day 7. Conclusion: A single-dose pre-operative antibiotic is as effective as three perioperative doses in preventing SSI in laparoscopic cholecystectomy for symptomatic, uncomplicated gallstone disease. Our study did not associate port-site SSI with a positive bile culture.

The bacteriological profile of infections in severely burned patients: a retrospective study in the intensive care unit of Hassan II university hospital, FEZ

The bacteriological profile of infections in severely burned patients: a retrospective study in the intensive care unit of Hassan II university hospital, FEZ

Bacteriological and Antibiotic Susceptibility Profile of Urinary Tract Infection among Online Motorcycle Drivers in Jakarta, Indonesia

Bacteriological and Antibiotic Susceptibility Profile of Urinary Tract Infection among Online Motorcycle Drivers in Jakarta, Indonesia

Epidemiological aspects and resistance profile of bacteremia in the region of Marrakech

Introduction: bacteremia in hospitals is a major public health problem that can lead to mortality and morbidity. The aim of our study is to establish the bacteriological profile of bacteremia and the state of antibiotic resistance in order to optimize probabilistic antibiotherapy at the Avicenna military hospital in Marrakech. Materials and methods: This is a descriptive retrospective study spanning a period of 5 years (January 2019 - December 2023), conducted in the laboratory of bacteriology-virology and molecular biology of the Avicenna Military Hospital in Marrakech, on the all bacteria isolated from blood cultures in hospitalized patients. Results: During this period, we collected 839 blood cultures, 140 of which resulted to isolate a bacterium. The predominant causative organism was Staphylococcus aureus (27%), The resistance profile of the strains was as follows: 3.57% for methicillin for Staphylococcus aureus and 50% for staphylococcus coagulase negative. Regarding Enterobacteria; 30.28% were producing ESBL, ciprofloxacin resistance was involved in 41.8% of Enterobacteria while all strains were sensitive to kanamicin. The resistance of Acinetobacter baumanii was 100% to ciprofloxacin, and 91% to ticarcillin, ceftazidime, imipenem and amikacin while remaining 100% sensitive to colistin. Conclusion: surveillance of bacteria’s epidemiological characteristics and their antibiotic susceptibility profile should be continuous for appropriate adaptation of initial empirical treatment of bacteraemia.

Incidence of drug resistance bacterial isolates from different Pyoderma cases attending at tertiary care hospital of Central Nepal

Background & Objectives: Pyoderma is defined as any purulent skin disease and represents infections in epidermis and dermis including hair follicles. Thus, this study was conducted to find out incidence and bacteriological profile of pyodermas as well as to determine their antibiotic susceptibility test of isolated bacteria. Materials and Methods: The samples were collected in dermatological OPD and sent the collected samples immediately to bacteriology laboratory for culture and sensitivity tests from December 2018 to May 2019 A.D. Antimicrobial susceptibility testing was done using Kirby–Bauer disc diffusion method and the results were interpreted according to the CLSI guidelines. Quality control of the test was done by standard ATCC strains of S.aureus (ATCC 25923), E.coli (ATCC 25922). Results: The incidence of pyoderma was 3.24%. Maximum cases were detected in the age group below 13 years of age. Primary pyoderma (61.8%) was more frequent when compared to secondary pyoderma (38.2%). The highest number of organism isolated was S. aureus (24.7%) from different cases of pyoderma followed by E. coli (8.2%), CONS (6.1%) and Klebsiella pneumoniae (5.1%) whereas other organisms were found in less number. Gram positive bacteria mainly S. aureus and other isolates for the cause of pyoderma were mostly resistant to penicillin (100%), ciprofloxacillin (75.0%) and clindamycin (79.2%). Gram negative E. coli (8.2%) was second most bacterial isolates and others GNB isolates were (14/63) where resistance pattern were seen more (75.0%) in each ceftrixone and imipenem. Conclusion: Incidence of pyoderma was low among the patients visiting to the Dermatology Department. Antibiotic resistance is increasing day by day; therefore, routine culture and sensitivity tests are essential to guide proper treatment procedure.

Cytobacteriological testing of drainage pus from peritonsillar abscess is not contributive in clinical practice: A STROBE analysis

PurposePeritonsillar abscess (PTA) is a frequent pathology. Treatment consists in drainage of the collection, associated to probabilistic antibiotic therapy. The usefulness of cytobacteriological testing (CBT) of the drainage pus is controversial. Material and methodsA retrospective study of patients managed for PTA between 2013 and 2020 in our university hospital was performed. The main objective was to assess the usefulness of CBT in the management of PTA. The secondary objectives were to determine the bacteriological profile involved in the onset of PTA and to assess the rate of bacterial resistance to antibiotics prescribed on a probabilistic basis. ResultsThe study included 207 patients: 70 outpatients (33%) and 137 inpatients (67%). Probabilistic antibiotic therapy was implemented in 100% of patients. CBT was performed systematically and was negative in 106 patients, revealing oropharyngeal flora in 40% of cases, polymicrobial flora in 50% and sterile samples in 10%. In the 101 patients with positive CBT, the bacteria isolated were penicillin-sensitive in 99%. All patients were successfully treated. In the light of the bacteriological results, no changes were made to the probabilistic antibiotic therapy introduced on admission. ConclusionCBT on drainage pus had no impact on the management of PTA. CBT is therefore unnecessary in patients with no comorbidities and no signs of severity at admission.

Umbilical cord blood culture for early onset sepsis in preterm infants.

Umbilical cord blood culture (UCBC) may have a diagnostic utility for early onset sepsis (EOS) detection in preterm infants. It may prevent sampling the newborn and collect a higher volume of blood for pathogenic identification. Retrospective analysis at a tertiary care center in Canada of preterm infants ≤ 34 0/7 weeks' gestation with UCBC taken at birth. Of 505 admitted infants, 195 had UCBC. 170 UCBCs were negative; 44 of these had also negative neonatal blood culture (NBC). No infants with negative UCBC showed EOS symptoms in the first week of life. 25 UCBCs were positive: 18 were contaminants (all with negative NBC) and 7 were confirmed as EOS. 18 infants with UCBC contaminants remained asymptomatic. 7 EOS cases were identified, with varying bacteriological profiles; 5 displayed sepsis symptoms while 2 were asymptomatic. Risk of EOS increased with prolonged rupture of membranes. UCBC effectively detected EOS establishing it as a method with possibly better diagnostic performance than NBC in high-risk neonates. Further studies are needed to improve UCBC technique and lower contamination rates. Umbilical cord blood culture has a higher bacterial identification rate than peripheral venous blood culture for the early identification of early-onset sepsis in preterm infants. Umbilical cord blood cultures that showed no growth were reliable predictors of not developing early onset sepsis. Umbilical cord blood culture should be considered as part of the evaluation for early onset sepsis in the preterm infants.

BACTERIOLOGICAL PROFILE OF CLINICALLY SUSPECTED SEPTICEMIA AMONG NEONATES AND THE ANTIBIOTIC SUSCEPTIBILITY PATTERN OF THEIR ISOLATES: A CROSS-SECTIONAL STUDY IN A TERTIARY CARE HOSPITAL OF JHARKHAND

Objectives: Neonatal septicemia is a generalized bacterial infection that occurs during the first 4 weeks of life and is one of the four primary causes of neonatal mortality and morbidity in India. This study aims to determine the bacteriological profile and antibiotic sensitivity patterns of isolates from blood cultures of suspected septicemic neonates in a tertiary care hospital. Methods: Two hundred and twenty-eight blood samples were collected and processed from patients in accordance with standard protocol. The antibiotic susceptibility of the isolates was determined by the disk diffusion method according to Clinical and Laboratory Standards Institute recommendations. Results: Blood culture results were positive in 44.7% of the patients. Late-onset sepsis was present in 53.92%, and early-onset sepsis was observed in 46.08% of the cases. The best overall sensitivity among Gram-negative isolates was to Amikacin, followed by Gentamycin and Meropenem. Gram-positive isolates had sensitivity to Chloramphenicol, tetracycline, Linezolid, Tetracycline, Vancomycin, and Piperacillin. Conclusion: The most common causes of newborn sepsis in this study were Gram-negative organisms (Klebsiella pneumoniae, Escherichia coli, and Citrobacter freundii) and Gram-positive organisms (Staphylococcus aureus), the majority of which are antibiotic-resistant.

Bacteriological profile and antibiotic susceptibility pattern of neonatal septicemia in a tertiary care hospital

ABSTRACT Background: Among the various reasons for neonatal mortality, septicemia continues to be a major cause of neonatal morbidity and mortality worldwide. Incidence varies from country to country, but it is much higher in developing countries than in developed countries. In India, the incidence of blood culture-proven sepsis was reported as 8.5 per 1000 live births. The bacteriological profile for causative organisms of neonatal sepsis differs significantly between developed and developing countries. Klebsiella pneumoniae is the most common bacterial agent in developing countries. This study was undertaken to determine the common bacterial agents causing neonatal sepsis from the tertiary care hospital and their antibiotic susceptibility and resistance. Methods: About 150 blood samples of suspected cases of neonatal septicemia admitted in NICU. Bacterial identification and speciation were done by standard laboratory procedures. Isolates and susceptibility were performed on Automation system. Results: Ninety-one babies presented within the first 72 h of life constituting early-onset sepsis, whereas 59 presented as late-onset sepsis. Among the 150 samples, blood culture was positive in 47 samples. Out of 47 culture-positive cases, 27 (58%) were Gram-negative bacilli, 18 (38%) were Gram-positive cocci, and two (4%) were fungal isolate. K. pneumoniae was the most commonly isolated Gram-negative bacilli. Conclusion: Neonatal septicemia is a life-threatening emergency and rapid treatment with antibiotics is mandatory for favorable outcomes. There is also a need for regular periodic surveillance of the causative organisms of neonatal sepsis as well as their antibiotic susceptibility patterns to inform the choice of empirical antibiotic prescription while awaiting blood culture results.

Bacteriological Profile and Antimicrobial Resistance of Semen Infections among Infertility Cases before and during COVID-19 Pandemic: A Comparative Study

Bacteriological Profile and Antimicrobial Resistance of Semen Infections among Infertility Cases before and during COVID-19 Pandemic: A Comparative Study

Bacteriological profile and antimicrobial susceptibility pattern of patients with urinary tract infections in a tertiary care hospital in a tertiary care center

Bacteriological profile and antimicrobial susceptibility pattern of patients with urinary tract infections in a tertiary care hospital in a tertiary care center

Bacteriological Profile of Common Lab Contaminants Responsible for Spoilage in Biological Laboratory

In biological laboratories, contamination can arise from various sources, including airborne particles, equipment, reagents, and human error. Common contaminants responsible for spoilage in biological laboratories include bacteria, fungi, virus etc. Bacteria are ubiquitous in the environment and can contaminate laboratory samples, cultures, and media. There is a possibility of transferring microorganisms from one laboratory to the other during the course of activities that involves moving of materials. In this present work we had been identify three bacterial species like Staphylococcus aureus, Klebsiella pneumonia, Enterococcus Sp. which responsible for lab spoilage. Therefore measures like cleaning of lab, using protective gears, autoclaving of glassware needed to minimize lab contamination.

Bacteriological profile, antimicrobial susceptibility, and factors associated with urinary tract infection in pregnant women.

Urinary tract infection (UTI) is a common bacterial complication in pregnancy. The study aimed to estimate the prevalence, risk factors, and bacterial etiology of UTI during pregnancy and determine the efficacy of antimicrobial drugs in treating UTIs. Urine specimens and clinical data were collected from pregnant women who attended primary health centers in Erbil, Iraq. All specimens were cultured on appropriate media and identified by standard microbiological methods. The pregnant women were grouped into symptomatic UTI group, asymptomatic bacteriuria group, and the control group. The agar dilution method was used to determine antimicrobial susceptibility. Among the 5,042 pregnant women included in this study, significant bacteriuria was found in 625 (12.40%) of the cases, and 198 (31.68%) had symptomatic UTI, of which 43.59% were diagnosed during the third trimester. Out of the 643 bacteria isolated, 33.28% were symptomatic UTI, of which 43.59% developed during the third trimester. There was a significant difference in the bacterial etiology between symptomatic UTI and asymptomatic bacteriuria (p = 0.002), as well as between cystitis and pyelonephritis (p = 0.017). The most common bacterial species isolated was Escherichia coli, which was susceptible to fosfomycin (100%), meropenem (99.45%), and nitrofurantoin (97.8%). Pregnant women are more likely to develop UTI in the third trimester. Escherichia coli is the predominant pathogen. The study suggests the use of fosfomycin, meropenem, and nitrofurantoin for the treatment of UTI. No Gram-positive isolates were resistant to daptomycin.

Bacteriological Analysis and Plasmid Profiles of Surfaces of Some Hospital Kitchen Equipment in Benin City, Nigeria

The surfaces of hospital kitchen equipment could be a major source of transmission of resistant pathogens to patients. Hence, the objective of this paper was to assess the bacteriological and plasmid profiles of surfaces of tables, sinks, chopping-boards, gas cookers and freezer handles hospital kitchen equipment in Benin City, Nigeria using appropriate standard microbiological techniques. Bacterial plasmids were isolated and separated using the agarose gel electrophoresis. Plasmid curing was performed using acridine orange. The antimicrobial sensitivity pattern showed that the bacterial isolates exhibited varying degree of resistance to the antibiotics. Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were highly resistant to the antibiotics, having multiple antibiotic resistances (MAR) index of 0.6. Micrococcus spp. and Klebsiella pneumoniae were the least resistant to the antibiotics. Plasmid analysis revealed the presence of single and multiple-banded plasmids with sizes ranging from 100-1000bp. After curing, all the isolates (except S. aureus, E. coli and P. aeruginosa) were sensitive to all the antibiotics, indicating a significant reduction in antibiotic resistance after curing. The results revealed that plasmids played a significant role in conferring resistance on the isolates. Regular cleaning and disinfection should be strictly observed in hospital kitchens to prevent outbreaks and spread of resistant pathogens in hospitals.