Bacterial Contamination Research Articles

Preparation and Characterization of Oxide Nanotubes on Titanium Surface for Use in Controlled Drug Release Systems.

Preventing or treating infections at implantation sites where the risk of bacterial contamination is high requires the development of intelligent drug delivery systems. The objective of this work was to develop a production method and characterization of fourth-generation oxide nanotubes on titanium grade 4 surface as a potential drug carrier. This study focused on the anodizing process; physico-chemical characterization using FE-SEM, EDS, and FTIR; in vitro corrosion resistance in an artificial saliva solution; and determining the drug release kinetics of gentamicin sulfate using UV-VIS. The anodizing process was optimized to produce fourth-generation oxide nanotubes in a fluoride-free electrolyte, ensuring rapid growth and lack of order. Results showed that the length of the oxide nanotubes was inversely proportional to the anodizing voltage, with longer nanotubes formed at lower voltages. The nanotubes were shown to have a honeycomb structure with silver particles co-deposited on the surface for antibacterial properties and were capable of carrying and releasing the antibiotic gentamicin sulfate in a controlled manner, following Fick's first law of diffusion. The corrosion resistance study demonstrates that the oxide nanotubes enhance the corrosion resistance of the titanium surface. The oxide nanotubes show promise in enhancing osseointegration and reducing post-implantation complications.

Are Aminoglycoside Antibiotics TRPing Your Metabolic Switches?

Transient receptor potential (TRP) channels are broadly implicated in the developmental programs of most tissues. Amongst these tissues, skeletal muscle and adipose are noteworthy for being essential in establishing systemic metabolic balance. TRP channels respond to environmental stimuli by supplying intracellular calcium that instigates enzymatic cascades of developmental consequence and often impinge on mitochondrial function and biogenesis. Critically, aminoglycoside antibiotics (AGAs) have been shown to block the capacity of TRP channels to conduct calcium entry into the cell in response to a wide range of developmental stimuli of a biophysical nature, including mechanical, electromagnetic, thermal, and chemical. Paradoxically, in vitro paradigms commonly used to understand organismal muscle and adipose development may have been led astray by the conventional use of streptomycin, an AGA, to help prevent bacterial contamination. Accordingly, streptomycin has been shown to disrupt both in vitro and in vivo myogenesis, as well as the phenotypic switch of white adipose into beige thermogenic status. In vivo, streptomycin has been shown to disrupt TRP-mediated calcium-dependent exercise adaptations of importance to systemic metabolism. Alternatively, streptomycin has also been used to curb detrimental levels of calcium leakage into dystrophic skeletal muscle through aberrantly gated TRPC1 channels that have been shown to be involved in the etiology of X-linked muscular dystrophies. TRP channels susceptible to AGA antagonism are critically involved in modulating the development of muscle and adipose tissues that, if administered to behaving animals, may translate to systemwide metabolic disruption. Regenerative medicine and clinical communities need to be made aware of this caveat of AGA usage and seek viable alternatives, to prevent contamination or infection in in vitro and in vivo paradigms, respectively.

Surveillance of household foodborne disease outbreaks in Zhejiang Province, China, 2010–2022

BackgroundFoodborne diseases are a growing public health concern worldwide and households are a common setting. This study aimed to explore the epidemiological characteristics of household foodborne disease outbreaks in Zhejiang Province and propose targeted prevention and control measures. MethodsDescriptive statistical methods were used to analyze household foodborne disease outbreak data collected from the Foodborne Disease Outbreaks Surveillance System in Zhejiang Province from 2010 to 2022. ResultsHousehold foodborne disease outbreaks showed an upward trend during the study period (Cox-Staurt trend test, p = 0.01563 < 0.05). These outbreaks mainly occurred from June to September, with 62.08% (352/567) of all reported outbreaks. The number of reported outbreaks varied in 11 prefectures, with a maximum of 100 and a minimum of only 7. Household foodborne disease outbreaks had a wide spectrum of etiologic factors. Mushroom toxins accounted for the largest proportion of all etiologies (43.39 %) and caused the highest proportion of hospitalization (54.18%) and death (78.26%). Such outbreaks are caused by accidently eating wild poisonous mushrooms. Bacterial infection (16.23%) was the second most common etiology, with Salmonella spp. and Vibrio parahaemolyticus being the primary pathogens. These outbreaks were caused by improper storage, improper processing or a combination of factors, and the foods involved were mainly aquatic animals, eggs and cooked meat. Other identified etiologies included plant toxins (9.52%), chemicals (7.23%), animal toxins (3.70%), and viruses (1.76%). Among the above-mentioned etiologies, mushroom toxins, bacteria, and animal toxins had seasonal characteristics. Analysis of regions and etiologies revealed that the proportion of various etiologies was different in 11 prefectures. Wild mushrooms (43.39%), aquatic animals (9.88%), and toxic plants (8.47%) were the top three foods involved in these outbreaks. The most common factors contributing to household foodborne disease outbreaks were inedibility and misuse (59.08%), followed by multiple factors (7.58%), improper storage (7.41%), and improper processing (7.41%). ConclusionsHousehold foodborne disease outbreaks were closely related to the lack of knowledge regarding foodborne disease prevention. Therefore, public health agencies should strengthen residents’ surveillance and health education to improve food safety awareness and effectively reduce foodborne diseases in households. In addition, timely publicity and early warning by relevant government departments, the introduction of standards to control the contamination of pathogenic bacteria in raw materials, and strengthened supervision of the sale of substances that may cause health hazards, such as poisonous mushrooms and nitrites, will also help reduce such outbreaks.

Differential drivers on the occurrence of antibiotic resistance genes in agricultural soils and crops: Evidence from the different fertilization regimes

Organic fertilizer application caused bacterial resistance contamination in farming systems has been widely documented, and long-term fertilization will exacerbate the migration of antibiotic resistance genes (ARGs) to crops and humans. However, it remains unclear whether a combined pattern of chemical and organic fertilizer application can arrest the high prevalence of ARGs in soil-crop. Here we investigated the ARGs occurrence under different fertilization regimes, and explored the mechanisms by which micro-ecological shifts and geochemical factors in modulating the ARGs fate in soil and crop. The results showed that the soil ARGs abundance was highest under 100% organic fertilizer, while the highest ARGs abundance in crops was observed at 100% chemical fertilizer. Application of organic fertilizers with more than 50% ration intensified the soil accumulation and migration of tetA, sul1, sul2 and macA genes. And, multidrug_transporter, macA and sul1 were co-shared in soil and crop, where ARGs potential hosts differed complete in the two, suggesting that these ARGs may be transferred across media by horizontal transfer. Procrustes analysis revealed that soil microbial community was significantly correlated with ARG hosts, and soil microbial evolutionary pathway was congruent with antibiotic resistance, suggesting that fertilizers affect soil ARGs abundance mainly by altering soil microbial composition and their ecological evolutionary trajectories. While, no significant correlation was observed between endophytes and crop ARG host. Structural equations demonstrated that soil nutrients and crop growth characteristics contributed largely to the prevalence of crop ARGs. This outcome will provide new insights into the high prevalence of ARGs in soil and crop, and offer fertilizer recommendations for effectively curbing antibiotic-resistance contamination in farming systems.

Assessment and Mitigation Strategies for Heavy Metals and Bacterial Contamination in Badshahpur Lake, Gurugram, India

Assessment and Mitigation Strategies for Heavy Metals and Bacterial Contamination in Badshahpur Lake, Gurugram, India

Application of augmented simplex‐centroïd mixture design in developing and optimizing new low‐cost microfiltration membranes from clays and cassava peels for bacteria removal

AbstractThe sharp increase in waterborne diseases due to bacterial contamination is limiting the supply of safe water in developing countries. This study focuses on the development and optimization of a low‐cost ceramic membrane based on natural resources and local waste for eliminating bacteria from water. The augmented simplex centroïd mixture design (ASCD) was used. The obtained optimal formulation consisted of 65% Ebebda clay (EB) 18.64% Koutaba clay (KG), and 16.34% cassava peel (PM) with a holding temperature of 1 100°C. The raw materials and membrane were characterized by chemical analysis using X‐ray florescence, X‐ray diffractometry, Thermal Gravimetric/Differential Scanning Calorimetry (TG/DSC), FTIR, scanning electron microscopy, SBET, porosity and flexural strength. The optimal membrane has 43.26% of porosity, 7.46 MPa of strength, 0.55 m2/g of specific surface area, an average pore diameter of 1.31 µm and a water permeability of 4 345,87 L h−1 m−2 bar−1. The ability of the membrane support to retain Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella sp and Staphylococcus aureus bacteria present in contaminated water was finally assessed. Retention tests showed 100% of P. aeruginosa, 97.37% of K. pneumoniae, 93.69% of Salmonella sp, and 90% of S. aureus, making this new, less expensive ceramic membrane a potential candidate for the water treatment.

Rapid detection of bactericidal efficacy of nanoparticles coated polyurethane foam by synchronous fluorescence spectroscopy

The ability of right-angled synchronous fluorescence spectroscopy (SFS) was explored to analyse the bacterial load in water treated with green synthesized silver nanoparticles (AgNPs) coated polyurethane foam (PUF). Gram negative (Escherichia coli, Pseudomonas aeruginosa) and Gram positive (Staphylococcus aureus) bacteria cultured in nutrient broth were diluted in autoclaved water containing NPs-coated PUF. The survival rate of S. aureus and E. coli lowered after ten minutes as compared to P. aeruginosa; however, after thirty minutes, the percentage viability dropped and recorded as 3.4%, 0.9%, and 0.1% for E. coli, P. aeruginosa and S. aureus respectively in the treated suspensions. No spectral change was observed in the fluorescence emission from the positive control and treated bacterial suspension owing to the masking effect of the emission from nutrient broth. In parallel, SF spectra recorded for directly picked bacterial colony dissolved in water showed remarkable drop in tryptophan emission after treatment with NPs-coated PUF. The SF data changes were assisted by hierarchical cluster analysis, which also made it possible to distinguish between positive control and treated bacterial suspensions. SFS has shown to be a reliable substitute for the culture plate approach for the quick identification of bacterial contamination in water.

ИЗУЧЕНИЕ БИОЛОГИЧЕСКОЙ ПОЛНОЦЕННОСТИ СПЕРМАТОЗОИДОВ В СПЕРМЕ С БАКТЕРИАЛЬНОЙ ОБСЕМЕНЕННОСТЬЮ

The aim of the study is to investigate the relationship between bacterial contamination of bull semen and the biological value of spermatozoa, as well as reproductive performance. The object of the study was Holstein bulls (n = 17). The material for the study was frozen-thawed semen of bulls in the amount of 61 samples. Microbiological studies of sperm doses were carried out using standard nutrient media in accordance with GOST 32222-2013. The total number of microorganisms was calculated by the number of grown colony-forming units (CFU/ml) in 1 cm3, with subsequent determination of their morphological pro-perties. Sperm motility and morphology were determined using the Argus CASA program. The state of DNA in spermatozoa was studied by the acridine orange test (AO test) using a fluorescence microscope. The study revealed a high positive correlation (r = +0.940**) between the number of microorganisms in sperm and pathology in individual sperm segments. Analysis of variance confirmed a statistically significant effect of the number of microorganisms on sperm morphology F = 123.2 (p=0.000). The correlation relationship between the fragmentation index and the content of microorganisms has a high value (r = +0.965**). Sperm motility negatively correlates (r = –0.768**) with the number of microorganisms (CFU/ml). In the presence of Mycoplasma spp in the samples, the number of cows with fruitful insemination was 35.5 % due to reduced motility, morphology and high fragmentation of sperm DNA (58 %). Sperm motility in samples containing Mycoplasma spp averages 24.5 %, while in samples without these microorganisms it is 48.9 %. Thus, it can be assumed that the presence of Mycoplasma spp in samples and high bacterial contamination rates lead to to lower reproductive performance in bulls.

Genome characterization of a multi-drug resistant Escherichia coli strain, L1PEag1, isolated from commercial cape gooseberry fruits (Physalis peruviana L.).

Foodborne infections, which are frequently linked to bacterial contamination, are a serious concern to public health on a global scale. Whether agricultural farming practices help spread genes linked to antibiotic resistance in bacteria associated with humans or animals is a controversial question. This study applied a long-read Oxford Nanopore MinION-based sequencing to obtain the complete genome sequence of a multi-drug resistant Escherichia coli strain (L1PEag1), isolated from commercial cape gooseberry fruits (Physalis peruviana L.) in Ecuador. Using different genome analysis tools, the serotype, Multi Locus Sequence Typing (MLST), virulence genes, and antimicrobial resistance (AMR) genes of the L1PEag1 isolate were determined. Additionally, in vitro assays were performed to demonstrate functional genes. The complete genome sequence of the L1PEag1 isolate was assembled into a circular chromosome of 4825.722 Kbp and one plasmid of 3.561 Kbp. The L1PEag1 isolate belongs to the B2 phylogroup, sequence type ST1170, and O1:H4 serotype based on in silico genome analysis. The genome contains 4,473 genes, 88 tRNA, 8 5S rRNA, 7 16S rRNA, and 7 23S rRNA. The average GC content is 50.58%. The specific annotation consisted of 4,439 and 3,723 genes annotated with KEEG and COG respectively, 3 intact prophage regions, 23 genomic islands (GIs), and 4 insertion sequences (ISs) of the ISAs1 and IS630 families. The L1PEag1 isolate carries 25 virulence genes, and 4 perfect and 51 strict antibiotic resistant gene (ARG) regions based on VirulenceFinder and RGI annotation. Besides, the in vitro antibiotic profile indicated resistance to kanamycin (K30), azithromycin (AZM15), clindamycin (DA2), novobiocin (NV30), amikacin (AMK30), and other antibiotics. The L1PEag1 isolate was predicted as a human pathogen, matching 464 protein families (0.934 likelihood). Our work emphasizes the necessity of monitoring environmental antibiotic resistance, particularly in commercial settings to contribute to develop early mitigation techniques for dealing with resistance diffusion.

Daya Hambat Asap Cair Tongkol Jagung Terhadap Bakteri Kontaminan Pada Tuna Loin Segar Diasosiasikan Dengan Kadar Histamin Dan TVB-N

The objective of this study is to analyse the inhibitory power of liquid smoke derived from corn cobs (Zea mays L.) at different levels of distillation on bacterial contaminants in fresh tuna (Thunnus sp) loins, which are associated with histamine levels and TVB-N values. The treatment in this research was the level of liquid smoke distillation, namely 3 times distillation, 2 times distillation, and 1 time distillation. The parameters tested included the inhibitory power of liquid smoke against bacteria, histamine levels, and TVB-N values, which were tested at the 18th hour at room temperature. This research was designed using the Completely Randomized Design (CRD) method. Data analysis used ANOVA and further tested with BNT. The research results demonstrated that the level of liquid smoke distillation had a significant influence (p5%) on all test parameters. The histamine levels produced ranged from 23,68 ppm to 46,18 ppm, while the TVBN values were in the range of 7,77mgN/100g to 11,05mgN/100g. Both were still in accordance with the SNI for Fresh Tuna Loin (2018). The greater the degree of distillation of liquid smoke, the more pronounced the inhibitory effect on bacteria and the greater the potential to inhibit bacterial growth in tuna loin.

“Burning scalp” syndrome: relevance, place in clinical taxonomy and therapeutic approaches

One of the sensitive skin types is the “burning scalp” syndrome. It is the most expressed subjective symptom complex of tactile sensations in the projection of the skin of the scalp. The most common manifestations of this syndrome, which significantly reduces the quality of patient’s life, are burning, itching, pain, trichodynia, redness like flushing and persistent erythema, mild exfoliation and hair loss sometimes. The “burning scalp” formation is heterogeneous and can be associated with comorbid dermatoses (seborrheic dermatitis, eczema, atopic dermatitis, psoriasis, rosacea, fungal and bacterial contamination, alopecia, etc.), organ and visceral pathology (diabetes mellitus, multiple sclerosis, tumors of the central nervous system, paraneoplastic conditions, immunodeficiencies, etc.), psychosomatic abnormalities (conversion disorders, sensory hypochondria, somatoform pruritus) and environmental influences (aquagenic pruritus, ultraviolet radiation). Now, there are no general recommendations for the “burning scalp” treatment. For long-term accompanying treatment and prevention, therapeutic cosmetics that normalize the microbiome and have antipruritic and anti-inflammatory effects are promising.

Tingkat Pengetahuan dan Sikap Pada Penerapan Hygiene Perorangan Pengolahan Makanan di Hotel Bumi Senyiur Samarinda

Personal hygiene is an action to maintain cleanliness and health, both physical and psychological. The goal is to maintain cleanliness, prevent contamination of dangerous bacteria from the body to the food being processed, and prevent disease. The aim of this research is to determine the level of knowledge and attitudes of food processing staff regarding the implementation of personal hygiene. This research is survey research, survey research is research that takes samples from a population and uses questionnaires as a data collection tool, one of the uses of survey research is for descriptive purposes. The sample is 12 food processing staff who work at the Bumi Senyiur Samarinda Hotel. Data collection uses questionnaires, questionnaires and observations. Analysis uses descriptive. Based on the results of descriptive analysis research, staff knowledge was 66.7% and staff attitude was 75%. For observation results, food processors got a value of 66.7%. In this way, the researchers found that the level of knowledge and attitudes of food processors at the Bumi Senyiur Samarinda Hotel generally obtained good results

The Influence of Dental Status and Blood Parameters Characterizing Endogenous Intoxication on the Timing of Childbirth.

Background and Objectives: Epidemiological and microbiological-immunological studies have led to the conclusion that periodontal disease may be a risk factor for preterm birth. The aim of this study was to investigate and identify the relationship of some hematological cellular biomarkers characterizing the chronic oral focus of infection with pregnancy outcomes and their impact on those outcomes. Materials and Methods: Clinical and laboratory tests were conducted on 100 pregnant women, grouped by full-term or preterm births, with the assessment of the following markers: DMF, CPI and PIRI, PHP, microbiological examination of periodontal pockets and amniotic fluid, WBS count, WBCSI, LGI, and NMR. A statistical analysis was carried out with SPSS Statistics version 19.0. Results: Women with preterm labor had higher-grade caries (CSL > 0.3), while women with full-term childbirth had moderate caries (CSL < 0.3). A satisfactory level of oral hygiene efficiency was found in 50% (group 1) and 38.1% (group 2) of the expectant mothers. The periodontal status by the PIRI showed tissue lesions in 20.7% (group 1) and 92.9% (group 2) of the women. The WBCSI was 2.27 ± 0.82 and 2.15 ± 0.68, the NMR was 9.29 ± 5.119 and 11.62 ± 7.78, and the LGI was 3.54 ± 1.1 and 3.73 ± 0.81 in groups 1 and 2, respectively. Comparative analysis of bacterial contamination of the amniotic fluid revealed the predominance of Fusobacterium nucleatum (64.3%), Tannerella forsythia (57.1%), Prevotella intermedia (50%), Porphyromonas gingivalis (57.1%), Staphylococcus aureus (45.2%), and Candida albicans (50%) in women with premature birth. Conclusions: In women with preterm birth, the values of the indices characterizing a chronic oral focus of infection evoke more significant correlations with the timing of delivery, which indicates the significant role of an oral focus of infection. The presence of microbial invasion of amniotic fluid may indicate the role of periodontopathogenic bacteria in pregnant women diagnosed with a risk of preterm birth.

Bacteriological and Physicochemical Analysis of Water from Different Sources in a Rural Community of Jos South Local Government Area (LGA), Plateau State, Nigeria

Surface and underground water polluted by microbes and chemicals exacerbates issues of water scarcity, given its importance to all life forms. This study investigated the impact of microbial and chemical pollution from water sources in the K-Vom community in Jos South Local Government Area of Plateau State, Nigeria. Four water samples from various sources underwent bacteriological and physicochemical analyses following the U.S. Environmental Protection Agency standard guidelines to assess their quality. Escherichia coli emerged as the predominant organism, with tap water showing the lowest contamination levels and well water, particularly from Angwan Madugu, displaying the highest bacterial counts. Physicochemical parameters generally met WHO standards, except for Total Suspended Solids (TSS) and Nitrates (Means: 0.14mg/L, 74.9mg/L) which exceeded recommended limits. Statistical analysis revealed non-significant differences for TSS, Nitrates, and Biochemical Oxygen Demand (BOD), suggesting overall compliance with international guidelines. Despite meeting certain standards, local water sources remain unfit for consumption due to bacterial contamination. Consequently, the study recommends the implementation of simple water treatment measures to mitigate health risks associated with waterborne diseases, emphasizing the urgent need for improved water quality management in the region.

Antimicrobial envelopes and endocarditis: innovative solutions for CIEDs infection

Abstract Introduction Endocarditis recurrence in patients with cardiac implantable electronic devices (CIEDs) remains a serious concern, demanding effective preventive measures. There is not always the possibility of extraction with a contralateral implant or the implantation of innovative devices. In recent years, two innovative technologies, TYRX and CanGaroo, have emerged as promising solutions to reduce the risk of endocarditis recurrence and improve patient outcomes. TYRX and CanGaroo are two cutting-edge medical technologies designed to address a critical concern in the management of patients with cardiac implantable electronic devices (CIEDs): the prevention and treatment of infected pockets. Both TYRX and CanGaroo offer unique approaches to mitigate this risk and improve patient outcomes. The TYRX is composed of a mesh envelope containing two antimicrobial agents, minocycline, and rifampin, that providing local and sustained delivery of antimicrobial agents directly at the device pocket. Instead, the primary function of the CanGaroo is to create a physical barrier between the CIED and the surrounding tissues, reducing the risk of bacterial contamination. The CanGaroo is particularly valuable in cases where patients have a higher risk of endocarditis recurrence due to underlying health conditions, compromised immune systems, or previous episodes of infection. By minimizing the direct contact between the CIED and adjacent tissues, the CanGaroo helps reduce the risk of bacterial adherence and biofilm formation. Objectives Educate the patient and caregiver to recognize signs of infection and avoid contamination. Improve training and education of cardiology nurse specialists for the prevention and recurrence of endocarditis on intra-operative management using these new devices and the post-operative course. Materials and Methods Database research and field evaluations. Outcome and Discussion TYRX and CanGaroo are groundbreaking technologies that demonstrate the potential to reduce the recurrence of endocarditis in patients with CIEDs. Their innovative designs and positive clinical outcomes highlight their importance as valuable tools in infection prevention strategies for CIED recipients. Conclusion Both TYRX and CanGaroo offer innovative approaches to prevent endocarditis recurrence in patients with CIEDs. By utilizing their unique mechanisms, these devices contribute to a multi-pronged infection prevention strategy. The TYRX envelope's localized antimicrobial delivery and the CanGaroo envelope's physical protection complement standard infection control measures during implantation. It is important to note that while TYRX and CanGaroo offer significant advances in infection prevention, they are not standalone solutions. A comprehensive approach, including stringent infection control protocols, patient selection, and diligent post-implantation monitoring, is crucial in reducing the risk of endocarditis recurrence effectively.tryx

IDENTIFIKASI BAKTERI PADA PRODUK DARAH THROMBOCYTE CONCENTRATE

TThe Thrombocte Concentrate component is the blood component that is most easily contaminated with bacteria due to its processing and storage at room temperature. Blood or blood products that are ready to be transfused must be safe and free from the risk of infection by infectious diseases and contamination by microorganisms. Sources of bacterial contamination can come from donor skin that is less than aseptic, donor bacteremia and processing of blood products. This type of research is Pre-Experiment. The design of this research is One-Shot Case Study. In One-Shot Case Study design. The sample in this study used 1 Thrombocyte Concentrate blood product. Then it was replicated into 10 samples for Nutrient Agar media and 10 samples for Mac conkey Agar media. From the results of research conducted on 10 samples of Nutrient Agar (NA) media, it was found that there was bacterial growth in all petri dishes on Nutrient Agar (NA) media. ), while in petri dish number 10 it was found that 10% of gram-positive bacteria were in the form of bacilli (rods). From the results of research conducted on 10 samples of Mac Conkey Agar (MCA) media, no bacterial growth was found in all of the MCA media petri dishes. There was contamination of Gram-Positive bacteria in the form of Cocci (Rounds) and Bacillus (Sticks) in Thrombocyte Concentrate blood products using NA media in 10 samples out of the total samples examined were 10 samples of NA media. There was no Gram-Negative bacterial contamination in Thrombocyte Concentrate blood products using MCA media in 10 samples from the 10 samples examined.

Platelet Storage-Problems, Improvements, and New Perspectives.

Platelet transfusions are routine procedures in clinical treatment aimed at preventing bleeding in critically ill patients, including those with cancer, undergoing surgery, or experiencing trauma. However, platelets are susceptible blood cells that require specific storage conditions. The availability of platelet concentrates is limited to five days due to various factors, including the risk of bacterial contamination and the occurrence of physical and functional changes known as platelet storage lesions. In this article, the problems related to platelet storage lesions are categorized into four groups depending on research areas: storage conditions, additive solutions, new testing methods for platelets (proteomic and metabolomic analysis), and extensive data modeling of platelet production (mathematical modeling, statistical analysis, and artificial intelligence). This article provides extensive information on the challenges, potential improvements, and novel perspectives regarding platelet storage.

Assessment of bacterial and parasitic contamination of fruits gathered from specific local markets in Addis Ababa, Ethiopia

BackgroundAround the world, food-borne illnesses are still a frequent and significant hazard to public health. Human infection outbreaks brought on by eating raw fruits have happened more frequently. Fruits have been connected in recent years to a number of outbreaks of food-borne illness around the globe, including in Ethiopia.ObjectiveTo assess bacterial and parasitic contamination of fruits gathered from specific local markets in Addis Ababa, Ethiopia.MethodsA cross-sectional study was carried out using fruits gathered from Addis Ababa local marketplaces. Convenient sampling were applied. With the assumption that each vendor provided 30 samples, a total of 120 fruit samples were gathered. Fruit samples were gathered in a plastic bag that had been sanitized and then brought to the lab for bacterial and parasitological investigation. All of the samples were checked for intestinal parasites and bacterial contamination. SPSS software version 25 was used to analyze the data. The Pearson's Chi-square test was used to assess categorical variables. The student's t-test was utilized to compare continuous variables, which were represented as the mean ± standard deviation. Using both univariate and multivariate analysis, odds ratios (OR) with 95% confidence intervals (CI) were computed. Statistical significance was defined as a P &amp;lt; 0.05.ResultsThere was a 100 (83.3%) level of bacterial contamination. While Salmonella and Shigella species were not recovered, S. aureus isolates 16 (13.3%) were the most common bacterial contamination, followed by E. coli isolates 8 (6.7%). The samples of bananas, mangos, papayas, and avocados from all four sites had total coliform (TCC) bacteria ranging from 2.1 × 103 to 3.2 × 104, 8.3x 102 to 1.8x 105CFU/g, 1.6x 102 to 3.7 x 104, and 1.2x 102 to 3.8 x 104 CFU/g, in that order. No parasites were found in this investigation.Conclusion and recommendationIt is usually recommended that customers in this research region wash and handle infected fruits properly to prevent bacterial illnesses. In addition, fruit dealers should have their bacterial contaminations routinely inspected. Large-scale research is advised to support this discovery.

Inhibition Rates of Disinfectants on Bacterial Isolated from Student Dormitory

Background: Although most of human life is spent inside, where bacteria are always present on things, little is known about the microbial variety of indoor habitats. Objectives: The major objective of this study was to determine the rate and types of bacterial contaminants in female and male student dormitory of Al-Kufa university campus, and to explore the inhibitory effect of certain disinfectants. This study was conducted for the period from October 2022 to Jun 2023. A total of 400 bacteriological swabs were collected aseptically from different sites of male and female student dormitory of Al-Kufa university. Swabs were streaked as soon as possible on blood agar and MaConkey agar plates and incubated overnight. Identification of bacterial species was based on standard bacteriological criteria. The inhibitory effect of 40% and 60% concentrations of three commercially available disinfectants; dettol (chloroxylenol), Al-Emlaq (Alkyl benzene sulfonate), and the house bleach (sodium hypochlorite) were explored by agar diffusion method. Data were statistically analyzed. Results: The results revealed that the overall bacterial contamination rate in student’s dormitories of Al-Kufa university campus was 78%. Male dormitory unit had significantly higher contamination rate compared to female dormitory unit (57.5% vs 42.5%). Bacillus spp. Was the most predominant contaminant in both male and female units. Al-Emlaq super gel (Alkyl benzene sulfonate) was found as the most effective disinfectant against the majority of bacterial isolates. Conclusion: Authorized and public awareness toward bacterial contamination in student's dormitories is recommended. The selection of an effective disinfectant should be based on its active ingredient and concentration.

Stimuli-Responsive Release-Active Dressing: A Promising Solution for Eradicating Biofilm-Mediated Wound Infections.

Biofilm-mediated wound infections pose a significant challenge due to the limitations of conventional antibiotics, which often exhibit narrow-spectrum activity, fail to eliminate recurrent bacterial contamination, and are unable to penetrate the biofilm matrix. While the search for alternatives has explored the use of metal nanoparticles and synthetic biocides, these solutions often suffer from unintended toxicity to surrounding tissues and lack controlled administration and release. In this study, we engineered a pH-responsive release-active dressing film based on carboxymethyl cellulose, incorporating a synthetic antibacterial molecule (SAM-17). The dressing film exhibited optimal mechanical stability for easy application and demonstrated excellent fluid absorption properties, allowing for prolonged moisturization at the site of injury. The film exhibited pH-dependent release of cargo, with 78% release within 24 h at acidic pH, enabling targeted antibacterial drug delivery within the wound microenvironment. Furthermore, the release-active film effectively eliminated repeated challenges of bacterial contamination. Remarkably, the film demonstrated a minimal toxicity profile in both in vitro and in vivo models. The film eliminated preformed bacterial biofilms, achieving a reduction of 2.5 log against methicillin-resistant Staphylococcus aureus (MRSA) and 4.1 log against vancomycin-resistant S. aureus (VRSA). In a biofilm-mediated MRSA wound infection model, this release-active film eradicated the biofilm-embedded bacteria by over 99%, resulting in accelerated wound healing. These findings highlight the potential of this film as an effective candidate for tackling biofilm-associated wound infections.