Pus Bacteria Research Articles

A Study to Evaluate the Antibacterial Activity of Homoeopathic Medicine Myristica Sebifera Mercurius Iodatus Rubrum Hamamelis Virginica Against Staphylococcus Aureus - in Vitro

Staphylococcus aureus is a Gram-positive, non-moving cocci found in grape-like clusters in the upper respiratory tract and skin. It is a member of the Bacillota and can grow without oxygen. Although it acts commensally, it can also become an opportunistic pathogen, causing skin infections, respiratory infections, and food poisoning. In 1880, Scottish surgeon Alexander Ogston discovered that Staphylococcus can cause wound infections after observing bacteria in pus from surgical abscesses. S. aureus is a common bacteria that can be found on the skin of the host, but most of its carriage occurs through the nasal passages and ears. The purpose of this study is to assess the antibacterial efficacy of the homoeopathic remedies Hamamelis virginica, Myristica sebifera, and Mercurius iodatus ruber against Staphylococcus aureus.As a result, the Agar Well-Diffusion assay is employed to screen the bacteria at 30C, 200C, and 1M potencies. Findings: Myristica sebifera 30C(11) mm, 200C (9 ) mm, 1M (10) mm; Mercurius iodatus ruber 30C (6 ) mm, 200C ( 8 ) mm, 1M ( 7) mm; Hamamelis virginica 30C(3 ) mm, 200C( 4) mm, 1M ( 5) mm; Mercurius sebifera in 1M showed maximum Growth inhibitory zone (GIZ) against Staphylococcus aureus following comparison with Vehicle control (dispensing alcohol or ethanol 90%) and Positive control (Amoxyclavatum).

Study of bacteria in pus with Antimicrobial Sensitivity Pattern

Material and methods: This retrospective study was conducted in the Department of Microbiology at Desh Bhagat Dental College January 2017 to June 2017 for a period of 6 month. The pus samples were collected from the patients who were visited in General hospital of Desh Bhagat University Mandi Gobindgarh Punjab, India, with skin and soft tissue infection. Bacteria were detected by culture and biochemical test and antibiotic susceptibility test done by disc diffusion method.

Immunoglobulin- and complement-coated bacteria in pus from peritonsillar abscesses.

Fifty-five samples of pus were collected from 51 acute, non-perforated, two spontaneously ruptured and two recurrent peritonsillar abscesses (35 males and 18 females; median age 18 years) and analysed regarding (i) aerobic and anaerobic bacteria (standard culturing), (ii) morphology of bacteria and inflammatory cells (direct microscopy of acridine orange-stained material), and (iii) the percentage of bacteria coated with immunoglobulins IgG, secretory IgA (SIgA) and IgM and complement cleavage product C3b (immunofluorescence assay). Seventy-one per cent of the abscesses harboured a mixed bacterial flora of various aerobes and anaerobes. In none of the cases with a single bacterial species (27 per cent) could immunoglobulin- or complement-coated bacteria be found. In abscesses with a mixed flora, 18 per cent harboured IgG-coated, 15 per cent SIgA-coated, five per cent IgM-coated and five per cent C3b-coated bacteria, respectively. All pus samples contained inflammatory cells in abundance but they were mostly deformed and only occasionally could intracellular bacteria be recognized. Insufficient immunoglobulin-coating of bacteria might be an important aetiopathogenic factor in the development of a peritonsillar abscess. Bactericide in the abscesses is accomplished chiefly by protective mechanisms not dependent on antigen recognition by antibodies.

Effect of metronidazole on Escherichia coli in the presence of Bacteroides fragilis: an investigation in mice.

The observation that, in rats, metronidazole exhibited antimicrobial activity against resistant Escherichia coli (when this was accompanied by susceptible Bacteroides fragilis) prompted us to attempt to reproduce this phenomenon in another species and under other experimental conditions. In experiment 1, mice injected intraperitoneally with an E. coli/B. fragilis mixture were treated with metronidazole, 250 mg/kg given by mouth at 0 and 10 h, or left untreated. At 24 h, viable counts of bacteria in blood and peritoneal washings were determined. In experiment 2, mice with 5-day-old subcutaneous abscesses containing E. coli and B. fragilis were also either given metronidazole as above or left untreated. At 24 h, viable counts of bacteria in pus were determined. Metronidazole affected neither the frequency with which E. coli persisted at the three sites, nor the viability of E. coli at these sites. This was so despite the fact that, in each of the sites, a significant B. fragilis kill was registered. Thus, pending elucidation of the mechanism by which the phenomenon operates, its non-appearance in these experiments cautions against the extrapolation of the original observations beyond the circumstances under which they were first made.