Relevance of the research topic. The systematization and generalization of a wide layer of both theoretical developments and practical achievements in the field of physical culture and sports serve as a basis for stating that maturity is the longest period of ontogenesis, characterized by the tendency for a person to achieve the optimal level of development of his spiritual, intellectual and physical potential. Against this background, the age of maturity in women, according to numerous data from representatives of the scientific community, is a period of motor deficit. The consequences of the latter include, first of all, the gradual unfolding of involutional processes, negative changes in the hormonal background, the appearance of excess weight, a decrease in the elasticity of connective tissue, the appearance of the first signs of structural and functional disorders of the spine and joints. The purpose of the article is to determine the level of physical fitness of women aged 36-40 with different types of posture. Methods. Theoretical analysis of special scientific and methodical literature, pedagogical testing, methods of mathematical statistics. The results of the work. The assessment of physical fitness of women in the second half of mature age showed the following. Firstly, it was found that according to tests of strength, endurance and flexibility, women aged 36-38 were more physically fit, performing pull-ups in a lying position mainly at an average level, tilting the torso forward from a sitting position and lifting the torso in a sitting position at a sufficient level. In contrast, the physical fitness of women aged 39-40 years was much worse, in particular, most of them performed lying pull-ups at a low level, torso bending forward from a sitting position and lifting the torso in a sitting position at an average level. Secondly, the comparison of groups of women aged 36-38 and 39-40 years, taking into account the type of their posture, did not reveal any differences in pairs of different ages of each type. At the same time, it was found that women with normal posture performed much better than the other groups in exercises such as flexion and extension of the arms in a lying position, pull-ups in a lying position, and women aged 36-38 years were superior in the test of lifting the torso into a seated position. Accordingly, women with a round, flat back and scoliotic posture were less physically prepared to perform these exercises.