Bachelor Males Research Articles

Male cooperation for breeding opportunities contributes to the evolution of multilevel societies.

A small number of primate species including snub-nosed monkeys (colobines), geladas (papionins) and humans live in multilevel societies (MLSs), in which multiple one-male polygamous units (OMUs) coexist to form a band, and non-breeding males associate in bachelor groups. Phylogenetic reconstructions indicate that the papionin MLS appears to have evolved through internal fissioning of large mixed-sex groups, whereas the colobine MLS evolved through the aggregation of small, isolated OMUs. However, how agonistic males maintain tolerance under intensive competition over limited breeding opportunities remains unclear. Using a combination of behavioural analysis, satellite telemetry and genetic data, we quantified the social network of males in a bachelor group of golden snub-nosed monkeys. The results show a strong effect of kinship on social bonds among bachelors. Their interactions ranged from cooperation to agonism, and were regulated by access to mating partners. We suggest that an ‘arms race’ between breeding males' collective defence against usurpation attempts by bachelor males and bachelor males' aggregative offence to obtain reproductive opportunities has selected for larger group size on both sides. The results provide insight into the role that kin selection plays in shaping inter-male cohesion which facilities the evolution of multilevel societies. These findings have implications for understanding human social evolution, as male–male bonds are a hallmark of small- and large-scale human societies.

Male Dispersal Pattern in Golden Snub-nosed Monkey (Rhinopithecus roxellana) in Qinling Mountains and its Conservation Implication

Golden snub-nosed monkey (Rhinopithecus roxellana) is one of the most endangered primate species found in China, exhibiting multilevel society consisting of several one-male-females together with their offspring units (OMU), and all-male units (AMU). Female dispersal patterns of the species within herd have been well documented, whereas those of the males within or between herds are still poorly understood. Our results based a long-term observation indicate that more than half of sub-adult males, and half of the deposed males that stayed a short period in OMU disperse between herds, three of them established their own OMU in new herd after the dispersal. Smaller number of the sub-adult and adult males, compared with adult females, stayed in natal herd, implying sub-adult males started dispersing and male-biased dispersal occurred between herds. High frequencies of resident males were wounded as their OUMs were taken over, and resident males co-operation defend bachelor males were found. Mating competition among males within the herd may have contributed to the scenarios of male-biased dispersal. The results also suggest that maintaining connection between isolated herds and establishing the corridors among the fragmented habitats for the species will greatly benefit increasing its gene flow and promoting conservation status.

Non-invasive endocrine monitoring indicates seasonal variations in gonadal hormone metabolites in dholes (Cuon alpinus).

To date, there is no information on reproductive endocrinology of dholes (Cuon alpinus). The objectives of the present study were as follows: (i) to characterize longitudinal profiles of gonadal steroids; and (ii) to examine the relationship between gonadal hormones and sexual behaviours in dholes. Three breeding pairs and two bachelor males were included in the study. Among these, four animals (2 males and 2 females; 4 years old) were imported from The Netherlands to Thailand 3 months before the study onset; the remaining individuals (3 males and 1 female; 5-7 years old) were native born. Faecal samples were collected 3-7 days/week for 12 months, extracted and assessed for gonadal hormone metabolites using a validated enzyme immunoassay. Observations of behaviour were conducted in 30min sessions, 3-5 days/week. For the three breeding males, testosterone was elevated (P<0.05) from October to January in the two imported males, whereas the concentration of steroid metabolites was high from April to June and from September to November in the native male. However, there was no clear seasonal pattern of reproductive hormone in the bachelor group. Oestrogen metabolite level of imported females was elevated for 9-12 days in January, followed by a rise in progestagen concentration. For native females, oestrogen metabolites were above the basal values in April and September, each of which was followed by a rise in progestagen concentration that remained elevated for 77 and 112 days, respectively. Sexual behaviours, including solicitation, mounting and copulations, were observed during the oestrogen peak in all females. Our findings indicate that reproductive seasonality of dholes may depend on the animals' origin and social group.

Alternative reproductive tactics and reproductive success in male Carollia perspicillata (Seba's short-tailed bat).

The use of alternative reproductive tactics (ARTs) is widespread in animals. Males of some species may change tactics depending on age, body condition and social environment. Many bat species are polygynous where a fraction of males only have access to fertile females. For polygynous bats, knowledge of the reproductive success of males using different ARTs is scarce, and it remains unclear how age of males is related to switching decisions between social statuses. We studied a large captive population of Carollia perspicillata, where males are either harem holders, bachelors or peripheral males. Using a multistate procedure, we modelled the age-related switches in reproductive tactics and in survival probability. From the model, we calculated the reproductive success and the frequencies of males displaying different reproductive tactics. As in mammals, the switch between social statuses is often related to age, we predicted that the transition probability of bachelor and peripheral males to harem status would increase with age. We show, however, that social status transition towards a harem holding position was not related to age. Reproductive success changed with age and social status. Harem males had a significantly higher reproductive success than bachelor males except between a short period from 3.8 to 4.4years of age where success was similar, and a significantly higher reproductive success than peripheral males between 2.6 and 4.4years of age. Harem males showed a clear decrease in the probability of maintaining social status with age, which suggests that senescence reduces resource holding potential.

Aiming low: A resident male's rank predicts takeover success by challenging males in Yunnan snub-nosed monkeys.

In many primate species that form one-male breeding units (OMUs), the threat of a takeover by a bachelor male represents a major challenge to group stability and individual reproductive success. In the case of snub-nosed monkeys, which live in large multilevel or modular societies (MLS) comprising several OMUs that travel, feed and rest together and as well as one or more all male units (AMUs), the process by which rival males challenge resident OMU males for access to females is poorly understood. From September 2012 to October 2013, we recorded 48 cases in which rival males visited an OMU in a MLS of Yunnan snub-nosed monkeys (Rhinopithecus bieti) inhabiting the Baimaxueshan National Nature Reserve, Yunnan Province, China. In 40 cases, rival males engaged in mild agonistic interactions (approaching, staring, teeth-baring and chasing) but failed to take over the group; we counted these visits as failed takeovers, recognizing that they may nevertheless allow rival males to assess the competitive ability of residents. During eight successful takeovers, however, there was severe physical aggression between challenging and resident males, with serious injuries to participants. We found that neither the number of adult and subadult females in an OMU, the number of non-pregnant, non-lactating adult females in an OMU, nor the rank of a resident male relative to other resident males in the MLS predicted which OMU a challenging male targeted for takeover. However, a resident male's rank significantly predicted whether takeover attempts were successful. Specifically, challenging males were more successful in displacing a lower-ranking resident male than a higher-ranking male. Given that a Yunnan snub-nosed monkey MLS may contain as many as 40 resident and 36 bachelor males, continued research is required to determine the set of factors that enable resident males to maintain high social rank and successfully defend their harems. Am. J. Primatol. 78:974-982, 2016. © 2016 Wiley Periodicals, Inc.

Parasitism and grooming behavior of a natural white-tailed deer population in Alabama

We assessed responses in grooming behavior to ectoparasite densities in naturally occurring white-tailed deer (Odocoileus virginianus) in southwest Alabama, and tested predictions of the stimulus-driven and programmed grooming models with respect to intraspecific body size and vigilance. Stimulus-driven grooming predicts greater tick densities would lead to an increase in grooming, whereas the programmed model predicts a higher rate of grooming would decrease tick densities. Within the programmed model, smaller individuals are predicted to groom more and host fewer ticks, and, due to increased vigilance, breeding males will groom less than females and bachelor males, and thus host more ticks during the rut. We used generalized linear models to determine males had a higher average tick density than females and exhibited complete separation of tick parasitism between non-rutting and rutting periods. Our results support the stimulus-driven grooming model as both fawns and yearlings had significantly higher deer ked and combined deer ked/tick densities than adults. We used Mann–Whitney U tests to conclude fawns oral groomed at a significantly higher rate than adults, even in the absence of allogrooming. Programmed and stimulus-driven grooming were not mutually exclusive, but rather ectoparasite and host dependent. Although individuals observed grooming were analyzed separately from harvested individuals, we infer that heavy deer ked densities may lead to higher grooming rates. We suggest white-tailed deer grooming for ticks should be considered within an evolutionary framework, and grooming for deer keds should be viewed as a proximate response to agitation. This is the first study to show that deer keds may have a greater influence in overall grooming behavior of deer than do ticks. We recommend that future studies should consider other ectoparasites along with ticks to understand their effects on grooming behavior in dimorphic terrestrial mammals.

Sexually selected lip colour indicates male group-holding status in the mating season in a multi-level primate society.

Sexual selection typically produces ornaments in response to mate choice, and armaments in response to male–male competition. Unusually among mammals, many primates exhibit colour signals that may be related to one or both processes. Here, we document for the first time correlates of facial coloration in one of the more brightly coloured primates, the black-and-white snub-nosed monkey (Rhinopithecus bieti). Snub-nosed monkeys have a one-male unit (OMU) based social organization, but these units aggregate semi-permanently into larger bands. This form of mating system causes many males to become associated with bachelor groups. We quantified redness of the prominent lower lip in 15 males (eight bachelors, seven OMU holders) in a group at Xiangguqing, China. Using mixed models, our results show that lip redness increases with age. More interestingly, there is a significant effect of the interaction of group-holding status and mating season on redness; that is, lip colour of OMU males undergoes reddening in the mating season, whereas the lips of subadult and juvenile bachelor males become paler at that time of year. These results indicate that lip coloration is a badge of (group-holding) status during the mating season, with non-adults undergoing facial differentiation, perhaps to avoid the costs of reproductive competition. Future research should investigate whether lip coloration is a product of male–male competition, and/or female mate choice.

Historic and Current Distribution, Abundance, and Habitats of Roosevelt's Sable AntelopeHippotragus niger roosevelti(Heller, 1910) (Cerartiodactyla: Bovidae) in Kenya

Roosevelt's sable Hippotragus niger roosevelti is one of Kenya's most distinctive and threatened large mammals. Historically, sable herds occurred in the vicinity of Taveta, and in the miombo and Diospyros woodlands of the coastal hinterland from the Tanzania-Kenya border northward for at least 210 km. Most of the historic distribution of sable in Kenya lies 15–35 km inland from the coast at 100–200 m altitude where mean annual rainfall is 800–1200 mm. In terms of numbers, however, most sable occurred in the higher and wetter Shimba Hills (150–460 m; mean annual rainfall 1000–1200 mm). Bachelor males sometimes moved >150 km inland. Much of the decline of the distribution and size of Kenya's sable population occurred during 1950–1980. Sable in Kenya not reported outside of Shimba Hills National Reserve after 1994. Geographic distribution of sable herds in Kenya declined from roughly 5000 km2 in 1884 to 70 km2 today (>98% decline in 132 years). The number of sable in Kenya was already small as of 1884, when there were probably 235 individuals in the mid-1970s to ca. 60 individuals in 2015 (>74% decline in 40 years). Given the low number, small distribution, and rapid decline, sable in Kenya qualifies as a nationally ‘Critically Endangered' species. Recommendations for the conservation of sable in Kenya are provided.

Vigilance behaviour of the year-round territorial vicuña (Vicugna vicugna) outside the breeding season: Influence of group size, social factors and distance to a water source

We conducted focal observations of vicuña, a year-around territorial mammal, to compare vigilance behaviour between territorial and bachelor males outside the reproductive season. We hypothesized that the time spent vigilant would depend on male social status, considering the potential effects of several variables: sampling year, group size, distances to the nearest neighbour and to a vega (mountain wetland). We fit GLM models to assess how these variables, and their interactions, affected time allocation of territorial and bachelor males. We found non significant differences between territorial and bachelor males in the time devoted to vigilance behaviour. Vigilance of territorial males was influenced by the sampling year and the distance to the vega. In turn, vigilance in bachelor males was influenced mainly by the sampling year, the group size and the distance to the vega. Our results suggest that sampling year and distance to the vega are more important than social factors in conditioning the behaviour of male vicuñas, during the non-reproductive season. Future studies of behaviour in water-dependant ungulates, should consider the influence of water and forage availabilities, and the interactions between group size and other variables.

Harem-holding males do not rise to the challenge: androgens respond to social but not to seasonal challenges in wild geladas.

The challenge hypothesis has been enormously successful in predicting interspecific androgen profiles for vertebrate males. Nevertheless, in the absence of another theoretical framework, many researchers ‘retrofit’ the challenge hypothesis, so that its predictions also apply to intraspecific androgen comparisons. We use a wild primate, geladas (Theropithecus gelada), to illustrate several considerations for androgen research surrounding male contests that do not necessarily fit within the challenge hypothesis framework. Gelada society comprises harem-holding males (that can mate with females) and bachelor males (that cannot mate with females until they take over a harem). Using 6 years of data from known males, we measured androgens (i.e. faecal testosterone (fT) metabolites) both seasonally and across specific male contests. Seasonal androgen variation exhibited a very different pattern than variation resulting from male contests. Although harem-holding males had higher testosterone levels than bachelors across the year, bachelors had higher testosterone during the annual ‘takeover season’. Thus, harem-holding males did not ‘rise to the challenge’ exactly when needed most. Yet, androgen profiles across male contests indicated that both sets of males exhibit the expected fT rise in response to challenges. Results from male geladas also support the idea that the context before (e.g. male condition) and after (e.g. contest outcome) a contest are critical variables for predicting hormones and behaviour.

Sneaker males are not necessarily similar to females in colour in a sexually monochromatic cichlid

In species with alternative reproductive tactics (ARTs), males employing different tactics usually have different appearance. The clearest difference is body size: bourgeois males that monopolize access to females are larger than sneaker males that steal fertilizations from them. Sneakers are also known to be often dull in colour compared with bourgeois males and rather resemble females. However, this typical colour pattern is unlikely in the Lake Tanganyika cichlid Neolamprologus mondabu: we observed sneaking by two distinctive colour morphs, namely, black (which is apparently conspicuous against the background) and white (which is apparently background-matching). Because breeding females are black, this observation indicates that one type of sneakers contrasts female appearance. In this study, we conducted field studies to determine the expression of body colour in relation to ARTs in this fish. Body colour was dependent on size, but not sex and maturation, with black individuals being larger than white ones. Together with the presence of intermediate grey colour expressed by intermediate-sized individuals, this fish could ontogenetically changes their body colour from white to black. Both sexes of black individuals occupied feeding territories, but white individuals were non-territorial, indicating that the black body signals the possession of a feeding territory. Sexually active females were invariably black, whereas sexually active males were both black and white in colour. Few of the largest black males held harems, which included several female territories, whereas the remaining males were bachelors with no female territories. These bachelor males invested more in testes than harem males, suggesting that bachelors employ sneaking tactics, which is corroborated by our sneaking observations. To our knowledge, this is the first study showing that sneakers are entirely dissimilar to females in appearance. Herein, we discuss why sneakers are dichromatic in relation to their life histories.

Factors affecting low resident male siring success in one-male groups of blue monkeys

In species that live in one-male/multi-female groups, resident males have more access to females than do bachelor males and should have a within-group reproductive advantage. We used a genetic analysis of 13 microsatellite loci to assign paternity to 111 offspring born over 10 years in 8 groups of wild blue monkeys. Resident males sired a maximum of 61% of the offspring conceived in their groups, indicating that despite their greater access to females, residents lost a substantial number of offspring to outsiders. A resident was less likely to sire an offspring when multiple females were in conceptive estrus, suggesting that it is difficult to monitor many fertile females simultaneously. Moreover, multiple estrous females likely attract competitor males, whose presence also decreased the probability that a resident sired an offspring. The negative effect of intruders on resident siring success may occur because females prefer competitors or because an increase in the number of intruders increases the challenge of effective mate guarding by a resident, leading him to miss rare mating opportunities. Tenure length did not affect resident siring success. Identifying the factors affecting patterns of paternity within species will help us to better understand the considerable variation in resident male siring success that occurs in one-male groups.

Males collectively defend their one‐male units against bachelor males in a multi‐level primate society

Group-level male-male co-operation, which has been documented in several primate and non-primate societies, may be mutualistically advantageous to the participants when confronted with threats such as takeovers and cuckoldry by external males. Co-operation among members of distinct social units-while universal among humans-is extremely rare in non-human primates. We present the first observations of collective action or co-operation among males of different one-male units (OMU) in a multi-level society of Rhinopithecus roxellana. A total of 59 instances of male co-operation were recorded. Male co-operation included coordinated chasing, joint vigilance, and patrolling behavior directed at lone adult males trying to enter an OMU. Male co-operation was significantly more frequent during the mating season when the risk of incursions and extra-group paternity was higher. Paternity of infants born in the subsequent birth season and kin relationships among resident males were identified using microsatellite genotype. All infants were sired by OMU males, which we interpret as possible evidence for their success at thwarting mating attempts by satellite males. OMU males were principally unrelated suggesting that male co-operation is best understood in terms of the mutual direct benefits individuals obtain through collective action. Our findings lend support to the bachelor threat hypothesis in which the cooperative behavior of several individuals is more effective than the lone action of a single individual in providing mate defense. Our research has implications for understanding male bonding, higher-level collective action, and the evolution of social co-operation in human societies.

Sex‐specific participation in inter‐group conflicts within a multilevel society: the first evidence at the individual level

Inter-group conflicts are common among many group-living animals and involve potentially complex motivations and interactions. Mammals living in multilevel societies offer a good opportunity to study inter-group conflicts. This study is the first to explore the function of sex-specific participation during inter-group conflicts within a multilevel society at the individual level. The Sichuan snub-nosed monkey (Rhinopithecus roxellana) is an endangered seasonal breeding species living in a multilevel society. From Sep 2007 to May 2008 we recorded 290 inter-group conflicts of a free-ranging provisioned band of R. roxellana in the Qinling Mountains of China to investigate the function of individual aggression during inter-group encounters. Our findings show that adult males were the main participants in inter-group conflicts, while females took part in them only occasionally. The male participation rate during the mating season, when adult females were estrous, was significantly higher than that during the non-mating season. Furthermore, males directed their aggression to other males, and directed more intense aggression towards bachelor males than towards other resident males. For both sexes, the participation rate as initiators was higher in the winter than that in the spring; and there was a significant positive correlation between group size and the participation rate as initiators. Our results suggest that inter-group aggression in Sichuan snub-nosed monkeys is linked to both mate defense and resource defense.

Detection of estrus in Indian blackbuck: Behavioural, hormonal and urinary volatiles evaluation

The determination of the reproductive status is one of the most important factors for effective wild life conservation and management, and effective use of assisted reproductive techniques like artificial insemination, in vitro fertilization/embryo transfer depends on the knowledge of the basic reproductive physiology. In this context the reproductive status of female blackbucks (Antelope cervicapra L.) was assessed by behaviour and determination, sex steroid hormones in faeces and urinary volatile compounds. The male and female blackbucks exhibited as many as 31 different reproductive/courtship behaviour patterns. Particularly, the males showed a more extensive repertoire: i.e. 23 behavioural patterns by territorial males, 11 by bachelor males and 4 by females. The behaviours such as, mounting, Flehmen, clockwise and anticlockwise movements were significantly higher in male blackbuck when exposed to estrus. By contrast, such courtship behaviours were completely absent in male when exposed to diestrus. It clearly indicates that, the estrus female produces specific chemical cues (pheromone) through urine, which would involve in attracting the conspecifics. In addition, the average faecal oestrogen concentration was significantly higher (p<0.05) during the estrus faecal than the proestrus and diestrus periods. In contrast, the faecal progesterone concentration was significantly higher (p<0.05) during the diestrus faecal sample than that of proestrus and estrus faecal sample. Twenty-eight volatiles are identified, across the three reproductive phases (i.e. proestrus, estrus and diestrus) of sexually mature and prepubertal females. Amongst, the compounds 2-methyl-3-butyn-2-ol, 3,7-dimethylnonane, 3-phenyl-2-propen-1-ol and 2-hydroxybenzoic acid occurred only during estrus which may be considered as marker for detection of estrus which would ultimately help for artificial insemination in captive condition. The findings of the present study suggest that the non-invasive approaches like reproductive behaviours, faecal steroids and estrus-specific urinary volatiles could serve as good indicators for detection of estrus for blackbuck.

Social ‘predators’ within a multilevel primate society

Group living confers a variety of benefits to individuals, particularly in predator detection and defence. Hamilton's selfish herd hypothesis ( Hamilton 1971 , Journal of Theoretical Biology, 31, 295–311) posits that individuals come together to reduce their own risk of predation, and numerous studies have shown that predators affect both the grouping and spacing patterns of their prey. We suggest that this hypothesis is also useful for understanding group responses to a very different threat: that posed by nonbreeding, potentially infanticidal males. In such cases, males may act as a predator-like force on the grouping patterns of breeding individuals. We hypothesized that nonbreeding males, like predators, can affect the spacing patterns of conspecifics. Specifically, we examined the effect of bachelor males on both the grouping and spacing patterns of gelada, Theropithecus gelada , reproductive units. First, we demonstrated that the number of bachelors was positively correlated with the number of animals in a group. Second, and more importantly, we found that bachelors exerted an acute pressure on the spacing of individuals; as bachelors approached, breeding individuals moved closer to their nearest neighbours. By approaching other breeding individuals, reproductive males and females may dilute the costs of associating with bachelor males.

Reversed Sex Change in The Haremic Protogynous Hawkfish Cirrhitichthys falco in Natural Conditions

AbstractBi‐directional sex change has recently been reported in a range of reef fishes, including haremic species that were earlier thought to be protogynous (female to male). However, the occurrence of this phenomenon and the social conditions driving the reversion of males to females (reversed sex change) have been poorly documented under natural conditions. Reversed sex change is predicted to occur in low‐density populations where facultative monogamy is common. However, few studies have evaluated this over a long period in such populations. We documented the occurrence of bi‐directional sex change during a 3‐yr demographic survey of a population characterised by small harem sizes in haremic hawkfish Cirrhitichthys falco. New males were derived following a change in sex of functional females (secondary males; n = 3) and juveniles always matured first as females (n = 3). Thus, C. falco exhibited a typical protogynous sexual pattern, consistent with a range of haremic fish species. We observed reversed sex change in two males. In both cases, all the females disappeared from their harems and the neighbouring males expanded their territories to encompass the territories of the sex changers. However, bachelor males did not always revert to females. A dominant male experienced bachelor status twice but regained mating opportunities following the immigration of a female into his territory or by taking a female from a neighbouring harem. Thus, we conclude that bachelor males use reversed sex change as a facultative tactic to regain reproductive status in a haremic mating system. In addition, we discuss the influence of harem size upon occurrence of reversed sex change.

How do Southern House Wrens Troglodytes aedon musculus achieve polygyny? An experimental approach

I designed two experiments to evaluate how polygyny is achieved in Southern House Wrens (Troglodytes aedon musculus) breeding in a south-temperate population. In the first experiment, I manipulated territory attractiveness by adding high quality nesting sites (nest-boxes) to 50% of monogamous territories (n = 24) and evaluated if males can attract a second female when defending high quality resources. In the second experiment, I simulated high male mortality early in the breeding season by removing 30 territorial males in plots where wrens had been breeding in nest-boxes (n = 47 territories). The first experiment did not induce polygyny: although females switched from breeding in tree cavities to nest-boxes when boxes were erected on their territory, none of the neighbouring females who did not receive a box moved to breed as a secondary female. The male removal experiment did induce polygyny: of 21 experimentally widowed females, 38% bred as secondary females of neighboring males—who expanded their territories in the absence of a defending male—and accepted polygyny even in the presence of neighbouring territories held by bachelor males. Secondary females mated to polygynous males were rarely helped by the male while feeding nestlings, but primary and secondary females overlapped very little in the use of space. Hence, females mated to polygynous males may share parental care disproportionally but not territorial resources. Female attachment to territories and exclusive use of space together with male’s expansion of territories to achieve polygyny suggests that Southern House Wrens engage in sublease polygyny.

Colony size, sex ratio and cohabitation in roosts of Phyllostomus hastatus (Pallas) (Chiroptera: Phyllostomidae)

Phyllostomus hastatus bat is species broadly distributed over the Neotropical region, which uses as diurnal roosts caves, hollow trees, palm leaves and human buildings. Thirteen diurnal roosts of P. hastatus were analysed from 1990 to 2009 in several localities of Rio de Janeiro State, regarding environment (rural, urban or protected area), type of roost (hollow tree, basement or roof), sex ratio and cohabitation. A nocturnal roost was also analysed. Sex ratio of P. hastatus varied considerably among roosts what may be explained by the fact this species can roost alone, in couples, in harems or in groups of bachelor males. Phyllostomus hastatus was observed in cohabitation with three other species: Molossus rufus, Molossus molossus and Myotis nigricans. Due to the frequency of cohabitation observed between P. hastatus and species of the genus Molossus, one or more advantages for the members of this association may be expected. The simultaneous usage of a feeding roost by a group of bachelor males is unknown information in the literature, and may suggest that this kind of group may interact with each other even when away from their diurnal roosts.

Preliminary insights into the level of genetic variation retained in the endangered echo parakeet (Psittacula eques) towards assisting its conservation management

This study examined a suite of microsatellite markers, originally developed for other parrot species, for their usefulness in providing details about the amount of genetic variation that has been retained in the endangered echo parakeet of Mauritius. Biological samples were available from previous studies and management activities. Four microsatellite markers, originally developed for the African grey parrot, were characterized for use in the echo parakeet. Given its recent population bottleneck, a relatively high level of genetic diversity has been retained in the wild population (Ho = 0.57). The genetic data are examined for their value in the conservation management of this species, both captive and wild. A number of conservation and breeding management issues are highlighted which warrant further investigation, for example in selecting captive breeding pairs and bachelor males for public display. Whilst four mircosatellite loci can only provide a limited insight, they do provide useful information relating to the remaining echo parakeet population and highlight that further genetic studies would prove invaluable to the conservation management of this species.