Birth Of Baby Research Articles

“Hecho en México”: a media analysis of the first MRT baby

ABSTRACT 2016 witnessed the birth of the first baby conceived using mitochondrial replacement technology (MRT), a procedure conducted by an international team in a Mexican clinic. The story soon made it to the international press, where authorship was assigned to the USA team and Mexico was described as lacking regulation, thus a suitable location. The Mexican media offered a different version. The baby was stamped as “made in Mexico” and the Mexican physician involved was described as a remarkable citizen and bold scientist. The story served as a counter-narrative to the one circulating in the press and academic circles. It dismantled the notion of Mexico as a country with “no rules” and instead offered a story of a country producing cutting-edge technology and of a government ready to back up scientific progress. In this paper, we analyze the performative acts that transformed this breakthrough into a national success and we ask about the role the Mexican team had in this accomplishment. This analysis contributes to the conversation held within the field of postcolonial science and technology studies, regarding knowledge production, authorship, and the role of science stories in the process of nation building.

Awareness and uptake of pre-conceptional genotype screening among pregnant women in South-South Nigeria

Sickle Cell Disease (SCD) is an inheritable genetic disease occurring worldwide. Nigeria is regarded as the epicenter of the disease, with a high number of SCD babies born annually. The risk of having an SCD baby remains high once there is a union between two heterozygous couple; therefore, it is imperative for intending couples to know their genotypes before marriage and conception. Currently, it is only the churches that insist on genotype before marriage. The result subsequently is a marriage between a heterozygous couple with a high possibility of the birth of an SCD baby. This cross-sectional study surveyed 430 consecutive consenting antenatal clinic clients presenting for booking in Central Hospital, Agbor, Delta State, Nigeria. The women completed a questionnaire with sections on sociodemographic attributes, awareness of their SCD and their genotype, awareness of their partner’s genotype, source of information, and ways to improve uptake of preconception genotype screening. The age range 25-34 years constituted 68.1% of the study population, with the majority of them (95.1%) of the Christian faith. Married women form 90.1% of the participants. Only 55.1% of the participants were aware of their genotype before conception. Higher education, being a Christian, marital status, and the type of marriage significantly affected patients’ awareness of their genotype. The majority of them heard about genotype screening from a health professional (30.5%), while the commonest reasons why many of them did genotype screening were for school admission 13.3%, for knowledge’s sake (12.1%) and as a requirement for marriage (24.7%). Some of the ways suggested to help increase the uptake of genotype screening include community meetings, text and WhatsApp messages, television and radio messages. Despite the fact that Nigeria remains the epicenter of SCD in the world, the uptake of preconception genotype screening is still low. The government, health workers, churches and marriage counselors, and the community have a major role to play in increasing the awareness and uptake of preconception genotype screening.

Commercialization of Colostrum Milk for the Benefit of Human Health in Gunupur City, Odisha

Colostrum milk is the first milk produce after the parturition or the baby birth. It is a necessary food for a new born baby for increase its immune system to protect against pathogen and boost the physiological performance, growth and mental development. Colostrum milk contains polysaturated fatty acids, growth factor, hormones, oligosaccharide, lactoferrin, a greater number of leukocytes (WBC cells) and high concentration of immunoglobulins. Colostrum milk provides passive immunity to the new born. People in the Gunupur block are establishing dairy farms and producing milk on a large scale. So, if we establish a factory of Colostrum milk then, we can easily collect the colostrum milk from the dairymen and supply the products by suitable transport medium to other states of India. The colostrum milk is highly proteinaceous product which is highly beneficial to the patients and build a new born babies’ immune system. My goal is to use government funding to commercialize colostrum milk in the Gunupur block and distribute it to nearby government hospitals and underprivileged citizens.

ANAEMIA, EXPECTANT MOTHERS AND DIETARY HABITS: ISSUES AND CONCERNS

The problem of anaemia among expectant mothers, instead of declining has increased over the years at the all India level and also in almost all the states of the country though differentially. These revelations come from the comparative analysis of the NFHS- 4 and NFHS- 5 data on anaemia released in the year 2000 and 2022 respectively. A review of the studies attributes the problem to inadequate nutritious food available to the expectant mothers during the period of pregnancy when the need of the body for iron and calcium increases for the proper growth of the foetus. In the absence of which the women are unable to bear the pressures of bleeding in the pre and post delivery period, leading to low weight baby birth and premature births, and at times resulting in high maternal mortality. This paper examines the status of anaemia among expectant mothers and its relationship with dietary habits.

THE EXPERIENCE OF MOTHERING IN MOTHERS OF PRETERM BABIES HOSPITALIZED IN A NEONATAL UNIT

A baby is considered preterm when it is born before 37 completed weeks of gestation, and it is necessary to admit it to a Neonatal Unit (NU) after birth. In addition to breaking with the fantasy of the imaginary baby, prematurity involves an anticipation of the process of recognizing oneself as a mother. This context of crisis provides a space of difficult elaboration around the experience of having a baby who was born prematurely and who is admitted to a hospital, bringing repercussions for the mother-infant relationship and for his psychic constitution. This work aims to investigate the experience of mothers of preterm babies admitted to the UN, based on Psychoanalysis. This is an integrative review study based on publications found in the Scielo, PePsic, LILACS and Redalyc databases in the last 10 years. The descriptors Prematurity, Psychoanalysis, Maternity and Neonatal ICU were used. 14 articles were selected for review of 67 identified general publications. It was found that the experience of mothers of preterm babies admitted to the NU is permeated by feelings of guilt, fear, frustration and ambivalence. The premature baby birth it is considered a risk factor for bonding, but it does not prevent mothers from finding strategies to exercise their role and bond. It is important to support the multidisciplinary team in the mothers' coping process and the psychologist's work in providing a listening space, allowing parents to talk about the experience and elaborate a place of subject for this baby.

Relationship between periodontal disease in pregnant women and premature birth: a comprehensive review

Initially, it should be noted that the birth of premature babies constitutes the fundamental cause of morbidity and mortality and morbidity soon after birth. There are several factors that imply risks and are directly related to its development, such as the use of psychoactive substances, infections of the genito-urinary tract and tobacco. smoke and other infections at a distance. Periodontal disease is considered an independent risk factor for premature low birth weight babies, which places active periodontal disease as an independent risk factor for the birth of premature and low birth weight babies. In this perspective, the objective of this work is to analyze periodontal disease as a risk factor for prematurely born babies. The methodology used in the development of the work is the literature review, with the use of articles from reference periodicals for the theme. It can be stated that in cases of periodontal disease, inflammatory stimuli can induce hyperirritability of the uterine smooth muscle, causing contraction of the uterus and cervical dilation, triggering premature birth. By constituting a chronic infection, it can influence the occurrence of premature birth indirectly or directly. However, it is important to understand that the treatment of periodontal disease is effective, reducing the incidence of premature birth.

Parental vaccine acceptance and refusal among neonatal period; single center retrospective study

INTRODUCTION: Vaccine hesitancy is a world-wide public health problem still persisting despite the mounting scientific evidence of the positive effects of vaccines on community health. The prevalence and causes vary based on cultural and sociological characteristics. In our study, we aimed to evaluate the situation of vaccine acceptance among parents who gave birth in our hospital. METHODS: This study is a retrospective descriptive study. The medical and vaccination records of mothers and babies born between June 2017 and June 2022 at Istanbul Medipol University Education and Research Hospital were examined. Socio-demographic data, mother's age, prenatal, natal and postnatal history, baby's gender, birth weight, vaccines and vitamin K status were recorded on the data form. SPSS software was used for statistical analysis. RESULTS: The total number of births in our hospital over a 5-year period was calculated as 15,417. The population of the study was made up of 192 (1.24%) cases, registered in epicrisis and infant birth records as vaccine refusal. The mean age of mothers was 30.72 (min: 20; max: 45) and the mean gestation week was 38.28 weeks (min: 32; max: 42; missing: 22). mean birth weight of the babies was 3008.78 grams and 56.25% of the babies were male. Considering vitamin K administration status of the cases, 61.73% (n: 121) did not accept. DISCUSSION AND CONCLUSION: Vaccination is the most effective method of preventing infectious diseases. Vaccination, one of the basic elements of preventive medicine in child health monitoring, should be carried out uninterruptedly. As a result, vaccine hesitancy rates can be reduced by partnering with each healthcare worker in solution strategies to increase vaccine acceptance. Education and awareness studies are also needed in this regard.

Spiritual Guidance Has an Effect on Decreasing The Level of Anxiety in Mothers During The First Stage of Labor

The birth process is a physiological event. Every mother who will give birth will experience pain as a manifestation of the baby's birth process. The effects of pain experienced by the mother will cause anxiety which often has a negative impact on birth outcomes. Many ways to deal with anxiety during labor are related to reducing pain, but only a few have researched the effect of spiritual guidance on reducing this pain. This study aims to assess the effect of spiritual guidance on the level of anxiety in mothers in the first stage of active labor. This study used a one-group pretest and post-test pre-experimental design on 71 mothers in the first active phase of labor. Anxiety levels were measured using the Hamilton Rating Scale for Anxiety (HSRA). The statistical analysis used is Wilcoxon. The results of the study found that there was a decrease in anxiety from before and after being given spiritual support to mothers in the first stage of labor. It is very important to provide childbirth support such as spiritual guidance to birth mothers in reducing their anxiety levels

Living Quran in the Context of Rural Communities: A Study on the Miracle of the Quran in Gentasari, Kroya, Cilacap

Abstract The Quran is a holy book containing various theological and social teachings and guides humans to the straight path. It’s just that when the Quran is present and consumed by the community, it experiences various responses which are implemented in many living Quran practices – positioning the Quran beyond its capacity as a text in their lives. These phenomena seem to be a concrete indicator of the expression often labeled to the Quran as a ṣāliḥ li-kulli zamān wa-makān or relevant to various situations and conditions. This article examines the practice of living the Quran in the Gentasari Village community, Kroya, Cilacap. Researchers used various instruments to explore data sources, such as interviews, observations, and documentation or studies of related documents. In clarifying the validity of the data, the researchers carried out an extension of participation and triangulation of sources and methods. From the research conducted, it can be seen that: 1) some of the Gentasari Village Community position the Quran as objects that have magical powers, 2) The implications of this view are implemented in their various receptions of the Quran, including: as a medium of treatment, as a medium of protection from spirits, repel reinforcements, as a medium to facilitate life’s problems – such as ease in death, ease of delivery, and requests for the birth of an ideal baby, who inherit the characteristics of figures in the Quran, such as Prophet Yunus, Prophet Muḥammād PBUH, Maryām, and so on.

Association between birth location and short-term outcomes for babies with gastroschisis, congenital diaphragmatic hernia and oesophageal fistula: a systematic review

BackgroundNeonatal care is commonly regionalised, meaning specialist services are only available at certain units. Consequently, infants with surgical conditions needing specialist care who are born in non-surgical centres require postnatal...

Environmental exposures and adverse pregnancy outcomes in Ethiopia: A systematic review and meta-analysis.

Maternal exposures to environmental hazards during pregnancy are key determinants of birth outcomes that affect health, cognitive and economic status later in life. In Ethiopia, various epidemiological evidences have suggested associations between environmental exposures such as household air pollution, cigarette smoking, and pesticide exposure and pregnancy outcomes such as low birth weight, preterm birth, and birth defects. This review aimed at generating summarized evidence on the association between maternal exposure to environmental factors (household air pollution, cigarette smoking, and pesticide) and pregnancy outcomes (birth weight, preterm birth, and birth defects) in Ethiopia. A systematic literature search was performed using PubMed, Google Scholar, and the Cochrane Library databases. All observational study designs were eligible for inclusion in the review. Quality assessment was conducted using the Newcastle-Ottawa Scale (NOS) quality assessment tools adopted for case-control and cross-sectional studies. The random-effects model was applied in computing the pooled estimates and their corresponding 95% confidence interval (CI). Funnel and Doi plots were used for detecting the potential publication bias. All statistical analyses were performed using comprehensive meta-analysis (CMA 2.0) and MetaXL version 5.3 software. The pooled estimates revealed that prenatal biomass fuel use increased the risk of giving a low birth weight baby by twofold (OR = 2.10, 95% CI: 1.33-3.31), and has no separate kitchen increases the risk of having low birth weight baby nearly by two and half times (OR = 2.48, 95% CI: 1.25-4.92). Overall, using biomass fuel as the main energy source for cooking and /or having no separate kitchen from the main house is 2.37 times more likely to give low birth weight babies (OR = 2.37, 95% CI: 1.58-3.53). Active cigarette smoker women were 4 times (OR = 4.11, 95% CI: 2.82-5.89) more prone to have low birth weight babies than nonsmokers; and passive smoker women were 2.6 times (OR = 2.63, 95% CI: 1.09-6.35) more risked to give low birth weight babies. It was also estimated that active cigarette smoker women were nearly 4 times (OR = 3.90, 95% CI: 2.36-6.45) more likely to give preterm birth babies. Pesticide exposure during pregnancy also increases the risk of the birth defect 4 times (OR = 4.44, 95% CI: 2.61-7.57) compared with non-exposed pregnant women. Household air pollution from biomass fuel use, active and passive cigarette smoking, and pesticide exposures are significantly associated environmental risk factors for low birth weight, preterm birth, and birth defects in Ethiopia. Therefore, Pregnant and lactating women should be aware of these environmental hazards during pregnancy. Promoting clean energy and improved and efficient stoves at the household level will help to reduce household air pollution-related adverse health effects. PROSPERO 2022: CRD42022337140.

Overview of Mother`S Body Mass Index (BMI) Before Pregnancy, Weight Gain, Upper Arm Circumference, and Hemoglobin Levels During Pregnancy, and Baby Birth Weight at The Cilandak District Health Center

Overview of Mother`S Body Mass Index (BMI) Before Pregnancy, Weight Gain, Upper Arm Circumference, and Hemoglobin Levels During Pregnancy, and Baby Birth Weight at The Cilandak District Health Center

Exploring the effect of cryopreservation in assisted reproductive technology and potential epigenetic risk.

Since the birth of the first baby by in vitro fertilization in 1978, more than 9 million children have been born worldwide using medically assisted reproductive treatments. Fertilization naturally takes place in the maternal oviduct where unique physiological conditions enable the early healthy development of the embryo. During this dynamic period of early development major waves of epigenetic reprogramming, crucial for the normal fate of the embryo, take place. Increasingly, over the past 20 years concerns relating to the increased incidence of epigenetic anomalies in general, and genomic-imprinting disorders in particular, have been raised following assisted reproduction technology (ART) treatments. Epigenetic reprogramming is particularly susceptible to environmental conditions during the periconceptional period and non-physiological conditions such as ovarian stimulation, in vitro fertilization and embryo culture, as well as cryopreservation procedure, might have the potential to independently or collectively contribute to epigenetic dysregulation. Therefore, this narrative review offers a critical reappraisal of the evidence relating to the association between embryo cryopreservation and potential epigenetic regulation and the consequences on gene expression together with long-term consequences for offspring health and wellbeing. Current literature suggests that epigenetic and transcriptomic profiles are sensitive to the stress induced by vitrification, in terms of osmotic shock, temperature and pH changes, and toxicity of cryoprotectants, it is therefore, critical to have a more comprehensive understanding and recognition of potential unanticipated iatrogenic-induced perturbations of epigenetic modifications that may or may not be a consequence of vitrification.

Apgar Score and the Amount of Blood of Mother in Labour in Delayed Cord Clamping Period

A delay in cutting the umbilical cord in infants can increase the Apgar score in infants who experience asphyxia. Besides that, it can prevent postpartum hemorrhage from occurring. Most deaths from postpartum hemorrhage occur during the first 24 hours after delivery. This increase in the Apgar value proves that the longer the delay in the umbilical cord is carried out, even until it doesn't pulsate, the better the baby's body will be, which results in an increase in hemoglobin in the baby's body. The population in this study were all mothers in the 3-4 stage of labor in the city area. Bandar Lampung. The sample is part of the number and characteristics possessed by the population with 120 maternal. It is known the length of time delayed cord Clamping is performed on babies born (<30 seconds) as many as 3 (2.5%) respondents, while umbilical cord clamping is delayed for 30-180 seconds as many as 117 (97.5%) respondents, the Apgar score in newborns in the category of mild asphyxia - not asphyxia as much as 100%, the amount of blood that came out <250 grams was 93 (77.5% ) respondents and the amount of blood that came out 250 grams were 27 (22.5%) respondents. Delayed cord clamping does not increase the incidence of asphyxia and bleeding in laboring mothers, so immediate umbilical cord clamping can be performed by observing the baby's birth condition.

ПРОБЛЕМА НЕВЫНАШИВАНИЯ БЕРЕМЕННОСТИ ПРИ АДЕНОМИОЗЕ: ПУТИ РЕШЕНИЯ

The purpose of this study is to substantiate the possibility of successful therapy of miscarriage with adenomyosis based on the study of a clinical case (the patient has a history of 7 unsuccessful IVF attempts). The analysis of this clinical case revealed that despite the presence of clinical signs of endometriosis, in particular, adenomyosis, such as copious, prolonged, painful menstruation with menarche, the absence of pregnancy for 12 months, provided regular sexual activity without the use of contraception by a married couple, unsuccessful IVF attempts in the anamnesis, the diagnosis of adenomyosis was made untimely. Although one of the signs of this disease may be the absence of spontaneous spontaneous pregnancy, despite regular ovulation, patency of the fallopian tubes and a normal spermogram in the partner, as was the case in this case. It is necessary to pay attention to the possibility of this pathology as a cause of infertility in infertile women. At the same time, each unsuccessful IVF attempt can also increase the likelihood of miscarriage, according to research. The adenomyosis revealed in this case apparently caused infertility . The treatment prescribed according to the current clinical recommendations, using progestogens in a continuous mode, in particular, dienogest at a dosage of 2 mg, showed a good result. It is important to take into account that the timing of drug administration and the time of its withdrawal are strictly individual, based on clinical criteria. In this case, as a criterion for drug withdrawal, the achievement of uniformity of the endometrium during ultrasound of the pelvic organs was used, which served as confirmation of the effective suppression of foci of endometriosis in the myometrium. To achieve this result, it took 15 months of hormone therapy with dienogest. The subsequent course of pregnancy was favorable and ended with the birth of a healthy full-term baby. This could also indicate the readiness of both the myometrium and the endometrium for fetal gestation and the normal course of pregnancy. This clinical case demonstrates the effectiveness of using dienogest at a dosage of 2 mg, prescribed with an individual selection of the duration of the course of therapy, in patients with adenomyosis in order to restore reproductive function.

National screening for Egyptian children aged 1 year up to 12 years at high risk of Autism and its determinants: a step for determining what ASD surveillance needs

This study aimed to provide a national estimate of the prevalence of the high risk of autism spectrum disorder (ASD) and their determinants. A national screening survey was conducted for 41,640 Egyptian children aged 1 to 12 years in two phases. Tools used were Vineland's Adaptive Behavior Scales, Modified Checklist for Autism in Toddlers, Gilliam Autism Rating scale, and Denver II Developmental screening test. The overall prevalence of children at high risk of ASD was 3.3% (95% CI:3.1%–3.5%). Children living without mothers in homes, suffered from convulsions (AOR = 3.67; 95%CI:2.8–4.8), a history of cyanosis after birth (AOR = 1.87; 95% CI:1.35–2.59) or history of LBW babies (AOR = 1.53; 95% CI:1.23–1.89) carried higher odds of being at high risk of ASD.

Knowledge and Problems Encountered During Teenage Pregnancy in Afgoi District, Somalia: A Descriptive Cross- Sectional Study

Pregnancy in a female under the age of 19 is referred to as teenage pregnancy or adolescent pregnancy. Due to the detrimental effects on the prospective bride and her offspring, teenage pregnancy has been referred to as a breach of human rights and a destructive practice. This paper investigates the knowledge and problems encountered during teenage pregnancy in Afgoi District, and the important implications for effective teenage pregnancy interventions. In the Afgoi District, this crosssectional study was carried out. Information was gathered from 150 teenagers. The data that was gathered thrown the use of the questionnaires that the respondents answered comprised determined the characteristics of the respondents, the extent of knowledge on early pregnancy among the respondents, and problems encountered about the experience of adolescent pregnancy. After analysing our data, we found; In about (PS=47.33) of the respondents were moderately knowledgeable about knowledge of Pregnancy and problems encountered. The current study found; (PS=45.33) of the respondents were moderately knowledgeable of Teenagers are more susceptible than mothers of average age to high blood pressure associated with pregnancy and its complications. However, the majority of the respondents are slightly knowledgeable that Smoking during pregnancy has been found to diminish birth weight and result in premature birth in babies (PS=20.67), while drugs and alcohol can have highly harmful effects on a mother and her unborn child (PS=22%).We found that most of the respondents sometimes encounter physical problems namely bleeding (WM=3.18); low Birth Weight (WM=2.99); premature birth (WM=2.93); miscarriage (WM=2.84); and hypertension (WM=2.62). Age, marital status, educational attainment, and source of income were positively correlated with the knowledge and Problem encountered in Teenage Pregnancy. Teenagers knew moderately about pregnancy and the issues that can arise during it. The majority of respondents also had a basic understanding that smoking during pregnancy has been found to lower birth weight and result in premature birth in babies, as well as the severe impact that drugs and alcohol may have on a mother and her unborn child. Age, marital status, educational attainment, and source of income were positively correlated with the knowledge and Problem encountered in Teenage Pregnancy. Therefore, in order to increase young teenagers' understanding of pregnancy, it is crucial to design a policy that outlines the promotion of educational awareness and improve their awareness of it through health education programs.

Effect of Oxytocin Massage on Breast Milk Production among Postpartum Mothers

Exclusive breastfeeding in the world is still very low. Various obstacles can arise in providing exclusive breastfeeding during the first six months of a baby's life. This causes a lack of provision of nutrients to infants, which can cause infant death. In a preliminary study conducted in the Postpartum Room at Bhayangkara Lumajang Hospital in February 2022, based on the results of interviews with 10 normal postpartum mothers, it was found that postpartum mothers complained that their milk did not come out and was not smooth and felt that their milk production was lacking, especially on the first day of the baby's birth. This study aimed to analyze the effect of oxytocin massage on breast milk production in postpartum mothers at Bhayangkara Lumajang Hospital. This type of research is pre-experiment by designing one group pre-post test design. Sampling using the method of simple random sampling with a sample of 36 respondents was carried out in August 2022. The instrument used for data collection was a checklist sheet. The results showed that almost all 36 respondents to produce milk after having oxytocin massage for postpartum mothers at Bhayangkara Lumajang Hospital in 2022 were in the current category with a total of 33 respondents (91.7%). It obtained a p-value=0.000, which means that oxytocin massage affects breast milk production (ASI) in postpartum mothers at Bhayangkara Lumajang Hospital.

P-728 Impact of calling mosaicism for a less stringent threshold on clinical pregnancies in more than 6000 PGT-A cycles

Abstract Study question How does calling mosaicism for a higher threshold affect euploidy, the chance of finding at least one euploid embryo and clinical pregnancy? Summary answer Increasing the threshold significantly increased euploidy percentage and the probability of finding at least one euploid embryo, as expected, but also the pregnancy outcomes. What is known already NGS technology has shed light on the occurrence of chromosomal mosaicism at the preimplantation stage of development. Evidence regarding clinical outcomes after transfer of embryos with putative mosaic results are accumulating (ESHRE Working Group on Chromosomal Mosaicism, 2022). A series of studies reporting the birth of healthy babies after the transfer of embryos with a chromosomal mosaic result on PGT-A (Greco et al., 2015; Kahraman et al., 2020; Viotti et al., 2019 and 2021), have been published. Data suggested lower implantation rates and higher miscarriage rates when mosaics were compared with euploid embryo transfers. Study design, size, duration This retrospective study covers a period from January 2017 to October 2022 and includes 6063 PGT-A cycles. A total of 18425 blastocysts were biopsied and analyzed with NGS. From January 2017 to December 2020, the cut-off value for reporting mosaicism and euploidy was %20; the rate of which was increased to 30% after January 2021. Chromosomes with PGT-A profiles that deviate from 2 copies by less than 30% were reported as normal. Participants/materials, setting, methods A total number of 12751 and 5674 blastocysts were biopsied between January 2017-December 2020 and January 2021-October 2022, respectively. PGT-A was performed on Ion Torrent S5 (Thermo Fisher Scientific). The mean female age for 20% and 30% cut-off groups were 36.7+-5.1, 35.9+-5.2, respectively. As this age difference was statistically significant, further analysis was performed in age subgroups < =38 and >38. Main results and the role of chance For PGT-A cycles with female age < =38, cycles with at least one euploid embryo in the 30% cut-off group increased from 74.0% to 77.5% whereas cycles with at least one mosaic embryo decreased from 40.8% to 37.0% (p = 0.0212; p = 0.0274). Cycles with at least one mosaic embryo without any euploid embryos decreased by 2% (9.2% to 7.2%) (p = 0.0522). The euploidy percentage out of all tested biopsies was significantly increased from 40.4% to 45.8% (p = 0.0001). For cycles with female age >38, ratio of PGT-A cycles with at least one euploid embryo increased from 28.1% to 30.3% in the 30% cut-off group but this was not statistically significant (p = 0.28). Cycles with at least one mosaic embryo were decreased from 15.1% to 10.8% and cycles with at least one mosaic embryo without any euploid embryo decreased from 9.5% to 5.6% in the 30% cut-off group (p = 0.006; p = 0.0018). The euploidy percentage was increased by 2.8% from 15.1% to 18.1% (p = 0.0115). The pregnancy outcomes were significantly higher for euploid embryos with the 30% cut-off compared to 20%, as clinical pregnancy was 64.5% vs. 59.1% and clinical pregnancy loss was 7.9% vs. 12.4% (p = 0.0022; p = 0.0011). Similarly, ongoing pregnancy was 5.2% higher, but non-significant. Limitations, reasons for caution These are the results of a single ART center which is using the same protocols for ovarian stimulation and endometrial preparation in the two periods above mentioned. Although not expected, minor changes may have occurred; similar practices have to be reported. Wider implications of the findings Mosaicism is undeniably a true biological phenomenon. However, trophectoderm biopsy and NGS are prone to create an artefactual false mosaicism. Relaxing the mosaicism calling rate by 10% may have added some better-quality blastocysts to the euploid pool giving the opportunity to embryologists of transferring blastocysts with higher implantation potential. Trial registration number Not applicable

P-270 The timing of intracytoplasmic sperm injection does not impact live birth rate and neonatal outcomes

Abstract Study question Does the timing of intracytoplasmic sperm injection (ICSI) relative to ovulation trigger impact live birth rates and neonatal outcomes? Summary answer ICSI timing relative to ovulation trigger impacts neither live birth rates nor neonatal outcomes. What is known already When conducting ICSI, the timing of the insemination relative to ovulation trigger is difficult to standardise in clinical practice, especially in busy ART laboratories. Flexibility when scheduling ICSI procedures would therefore be beneficial from a laboratory management perspective. However, the impact of this timing on live birth rates and neonatal outcomes remains inconclusive in the literature. Study design, size, duration This retrospective study included a total of 1547 consecutive single autologous embryo transfer cycles (maternal age 35.2 ± 4.8 years) performed at Fertility North between January 2017 and August 2021. ICSI was performed immediately after denudation without timing restrictions relative to ovulation trigger. A total of 422 (27.3%) transfers resulted in a live birth, including 415 singletons, 5 twin deliveries, and 2 lost to follow-up. Only singleton babies were included for neonatal outcome analysis. Participants/materials, setting, methods ICSI timings relative to ovulation induction trigger (mean ± SD 42.5 ± 1.4 hours, range 37.0-46.5 hours) were extracted from the electronic witnessing database. Cycles were grouped according to ICSI timing quartiles (groups Q1-4: <41.7, 41.7-42.7, 42.8-43.4 and 43.5+ hours). Logistic/linear regressions were used to assess impacts of ICSI timing on the subsequent live birth and birthweight outcomes in the singleton babies. Mixed effect model was used to account for clustering effect from repeat cycles by same patients. Main results and the role of chance Q1 group had significantly lower live birth rate (22.7%) but higher maternal age (37.3 ± 5.2 years) in comparison to Q2 (28.5% and 34.5 ± 4.6, P < 0.05 respectively), Q3 (30.2% and 34.8 ± 4.5, P < 0.05 respectively) and Q4 groups (27.7% and 34.4 ± 4.4, P < 0.05 respectively). However, following multivariate logistic regression adjusting for potential confounders including maternal age, body mass index, aetiology, sperm origin (ejaculated or surgical), sperm type (donor/partner), embryo stage at transfer (cleavage or blastocyst), and fresh/frozen transfer; observed differences in live birth rates became no longer significant (Q2 vs Q1, aOR = 0.982, 0.692-1.394, P = 0.919; Q3 vs Q1, aOR = 1.058, 0.884-1.266, P = 0.538; Q4 vs Q1, aOR = 0.969, 0.859-1.094, P = 0.615; Q3 vs Q2, aOR = 1.153, 0.830-1.600, P = 0.396; Q4 vs Q2, aOR = 0.963, 0.818-1.134, P = 0.649; Q4 vs Q3, aOR = 0.836, 0.601-1.164, P = 0.289). Gestational age and birthweight of singletons arising from 4 groups were also comparable (Q1 38.1 ± 2.2 weeks and 3209.1 ± 581.4 grams, Q2 38.2 ± 1.6 weeks and 3159.9 ± 508.2 grams, Q3 38.7 ± 1.8 weeks and 3304.7 ± 546.9 grams, and Q4 38.4 ± 2.0 weeks and 3297.5 ± 526.9 grams; P > 0.05 respectively). Multivariate linear regression further confirmed the absence of significant impacts (standardised coefficient = 0.001, P = 0.980) by ICSI timing on the birthweight of resulting newborns, taking into account gestational age and baby sex plus all above mentioned 7 potential confounders. Limitations, reasons for caution Our study is limited by its retrospective nature, where it is impossible to control all known and unknown confounding factors. The varied denudation duration as a result of different egg numbers was unable to be analysed, although ICSI was aimed to be performed immediately after completion of denudation. Wider implications of the findings Our findings indicate a wide time window in human oocytes to initiate fertilization via ICSI. This data offers reassurance in applying flexible ICSI timing without impacting live birth rates and neonatal outcomes. This is clinically valuable by providing evidence-based guidance to daily practice and patient counselling. Trial registration number not applicable