Baby Lima Bean Research Articles

Multi-Dimensional Liquid Chromatography of Pulse Triacylglycerols with Triple Parallel Mass Spectrometry

We analyzed ten pulses (the dried seeds of legumes), i.e., baby lima beans, black beans, black-eyed peas, butter beans, cranberry beans, garbanzo beans, green split peas, lentils, navy beans, and pinto beans, using three-dimensional liquid chromatography (3D-LC) with parallel second dimensions, LC × (LC + LC). We combined non-aqueous reversed-phase (NARP) chromatography as the first dimension separation, 1D, with argentation UHPLC for separation based on degree and location of unsaturation in the first second dimension, 2D(1), and multi-cycle NARP-UHPLC in the second second dimension, 2D(2). Pulses contained 1.9% to 2.7% lipids, except garbanzo beans, which contained 6.2% lipids. High-resolution, accurate-mass (HRAM) orbitrap mass spectrometry (MS) was used to perform lipidomic analysis of the 2D(2) and percent relative quantification, showing that the most abundant average triacylglycerol (TAG) molecular species across all pulses were PLL at 10.67% and PLLn at 10.45%. Common beans (Phaseolus vulgaris) were clustered together using principal component analysis (PCA), showing the highest levels of linolenic acid, C18:3, in molecular species such as PLnLn, LLnLn, and OLLn, with palmitic (P), C16:0, linoleic (L), 18:2, linolenic (Ln), 18:3, and oleic (O), 18:1, FAs. Calibration curves derived from interweaved sets of regioisomer standards allowed the absolute quantification of 1,2- and 1,3-regioisomers for a subset of TAGs.

Fast chromatography of pulse triacylglycerols

AbstractA short 10‐min ultra high performance liquid chromatography (UHPLC) method was used for separation and lipidomic analysis of triacylglycerols (TAGs) in 10 pulses: baby lima beans, black beans, black‐eyed peas, butter beans, cranberry beans, garbanzo beans, green split peas, lentils, navy beans, and pinto beans. The lipids extracted using chloroform/methanol averaged 1.9–2.7% across all pulses except garbanzo beans, which gave 6.2% lipids. TAGs were analyzed using dual parallel mass spectrometry (LC1MS2), areas were integrated using LipidSearch 4.2 software, and percent relative TAG compositions were calculated. Fatty acid response factors were calculated by comparison to calibrated GC‐FID, which were used to calculate response‐factor adjusted TAG compositions. Principal component analysis revealed that the pulses separated into three clusters, which were further highlighted using hierarchical cluster analysis. A subset of TAGs was quantified using calibration curves made from alternating sets of regioisomers in the non‐linear high concentration range (~2.5 nMol/ml to ~300 nMol/ml). A linear calibration curve for the sum of tocopherols detected by fluorescence was constructed with a coefficient of determination, r2, >0.99 for low concentrations (0.50–50 μg/ml), but tocopherols in pulses appeared to be inefficiently extracted. TAG regioisomers were quantified based on a Critical Ratio (CR), [AA]+/[AB]+, interpolated between the CRs of two pure regioisomer standards taken from alternating calibration standards. TAG mole percent relative compositions are reported for the first time for most pulses and the compositions are given with more detail and specificity than previously reported.

ISOLATION OF BABY LIMA BEAN (PHASEOLUS LUNATUS) PROTEINS FRACTIONS AND EVALUATION OF THEIR ANTIOXIDANT ACTIVITY

The aim of this work was to obtain fractions of baby Lima Bean Protein Concentrate (LBPC) from ( Phaseolus lunatus ) and to evaluate their antioxidant activity. LBPC was prepared by alkaline extraction and isoelectric precipitation at pH 4.5. LBPC was subject to gastrointestinal digestion simulation. LBPC was fractionated using a DEAE Affi-Gel Blue Gel column. LBPC presented a protein content of 77.20% with a protein solubility capacity ranging between 37.34% to 99.98%. The antioxidant activity was evaluated using the FRAP, DPPH and ORAC methods. LBPC cationic fractions presented a high antioxidant activity when using the ORAC method with values ranging between 0.47 to 3.37 ?mol TE/?mol of sample.

Spatiotemporal Attributes and Crop Loss Associated with Tan Spot Epidemics in Baby Lima Bean in New York.

Tan spot, caused by the pycnidial fungi Didymella americana and Boeremia exigua var. exigua, is a foliar disease affecting processing baby lima bean production in New York. Tan spot epidemics are prevalent, occur annually, and may result in substantial defoliation. The disease is controlled by the prophylactic application of fungicides to maximize green leaf area. Information on yield losses due to tan spot on baby lima bean yield and the benefits of fungicide applications is needed to justify investments in disease management. Four small-plot, replicated trials were conducted over 2 years in commercial baby lima bean fields to evaluate the efficacy of fungicides for tan spot control at Piffard and Leicester, NY. Applications of pyraclostrobin or boscalid significantly reduced tan spot incidence and severity compared with nontreated plots, and increased the number of leaves per stem. In 2016, the increase in green leaf area associated with fungicide application was also documented in canopy reflectance values at 830 nm. Despite the decrease in tan spot incidence and corresponding increase in crop health obtained from fungicides, this effect did not translate into significant increases in pod yield. This finding suggested that the relationship between green leaf area and yield is highly variable in baby lima bean. The spatial and spatiotemporal patterns of naturally occurring tan spot epidemics were also characterized in baby lima bean fields across western New York using disease incidence data collected in transects and grids. The spatial pattern of data collected in transects was analyzed using median runs analysis. Disease incidence data collected in two-dimensional grids were analyzed to quantify spatial pattern using spatial analysis by distance indices (SADIE). The association function of SADIE was used to quantify the spatiotemporal patterns of tan spot epidemics after crop emergence and at harvest. These findings suggested that tan spot is likely to initiate at relatively frequent, randomly positioned foci, and that subsequent, limited spread results in significant local aggregation. Hypotheses for inoculum sources and recommendations for tan spot control in baby lima bean fields in New York are discussed.

Genotypic characteristics in populations of Sclerotinia sclerotiorum from New York State, USA

AbstractWhite mould, caused by the fungus Sclerotinia sclerotiorum, is one of the most destructive diseases of beans globally. In New York State, USA, white mould causes substantial losses in soybean, snap, dry and succulent baby lima beans, which are grown successively in intensive crop rotations. Management strategies for white mould in these crops are reliant upon the prophylactic use of fungicides. No complementary information on the genetic structure of the populations of S. sclerotiorum in New York State, USA is available. Twenty isolates of S. sclerotiorum were collected from symptomatic bean plants within each of 10 fields across New York State, USA in 2014. Eight microsatellite (SSR) markers were used to characterise the genotypic diversity of the hyphal‐tipped isolates. Twenty‐four multilocus genotypes (MLGs) were detected within the population but one MLG was most prevalent. Although STRUCTURE analysis identified two subpopulations, these subpopulations were not associated with geographic location, suggesting no spatial structure to the population. In addition, the pathogen populations were predominantly clonal, with some evidence of infrequent outcrossing. These findings may assist in understanding the durability of management strategies for white mould and support the selection of representative isolates for host resistance screening for pathogen populations in the sampling area.

Physicochemical properties and in vitro digestibility of legume starches

The physicochemical properties and in vitro digestibility of starches from different cultivars (genus) of legumes, namely, black eye bean, baby lima bean, lentil, chick pea, small red bean, and mung bean, were analyzed. All legume starches had oval and spherical granules, with an average diameter of 18.6–27.8 μm. Baby lima bean starch, with high amylose content, high molecular weight (Mw) of amylopection, and low short amylopection branch chains (DP 6–12), showed high gelatinization temperature, gelatinization enthalpy (△H), final viscosity, setback viscosity, and pasting temperature. By contrast, chick pea starch, with low amylose content and Mw of amylopection as well as high short amylopection branch chains, exhibited low gelatinization temperature, △H, peak viscosity, breakdown viscosity, and pasting temperature. Baby lima bean starch was highly resistant to enzyme digestion. The contents of slowly digestible and resistant starches of cooked small red bean were significantly higher than those of other cooked starches.

Managing downy mildew in Delaware lima beans

Delaware is a lima bean powerhouse, competing with California for the top spot of green baby lima bean production. Downy mildew has the potential of doing the most widespread, epidemic damage to a crop in a single year. Researchers at the University of Delaware are busy developing new resistant cultivars and testing an online risk model for fields. Earn 0.5 CEUs in Integrated Pest Management by reading this article and taking the quiz at www.certifiedcropadviser.org/certifications/self‐study/802.

First report of Didymella americana on baby lima bean (Phaseolus lunatus)

A new foliar disease was observed on baby lima bean (Phaseolus lunatus) in fields across western New York State, USA. The disease occurred in 10 fields with variable incidence and severity. Symptoms were initially necrotic, tan spots on leaves with red to reddish brown irregular margins that coalesced to encompass the entire leaf and cause abscission. Pycnidia were observed within the lesions. Isolations from diseased leaves yielded several pycnidial fungi, including a Didymella species. These isolates were characterized by morphology and sequencing of multiple reference genes (internal transcribed spacer (ITS), partial actin, β-tubulin (tub2), translation elongation factor 1-α (TEF), 28S rDNA large subunit (LSU), rpb2 and calmodulin). A four gene phylogeny (ITS, tub2, LSU and rpb2) showed that the isolates from baby lima bean belonged to a well-supported clade that contained the type culture of Didymella americana. Pathogenicity of the isolates on three commonly grown cultivars of baby lima bean was confirmed. Symptoms that developed on inoculated plants were similar to those observed on diseased plants in the field. This is the first report of D. americana on baby lima bean.

Managing Pod Rot of Lima Bean Caused by Phytophthora capsici with Fungicides in the Mid-Atlantic Region

The baby lima bean (Phaseolus lunatus) is one of the most economically important crops for Delaware. Pod rot of lima bean caused by Phytophthora capsici is a relatively new disease that threatens production and profitability of lima beans grown in Delaware and the mid-Atlantic region. Insensitivity to the fungicide mefenoxam has made managing the disease challenging in recent years. In 2014, 12 fungicides in two trials were tested for their efficacy against P. capsici in lima bean. Results indicated that all fungicide treatments significantly reduced disease incidence compared to the control, with oxathiapiprolin achieving the best control in Trial 1 and fluazinam and mefenoxam achieving the best control in Trial 2. Fungicide treated plots also yielded significantly greater than controls in Trial 2. These results indicate that there are additional fungicides on the market with efficacy towards managing pod rot of lima bean and warrant further investigation for possible new labels. Accepted for publication 16 May 2016. Published 9 June 2016.

Pie Love You, Cake Do Without You

Pie Love You, Cake Do Without You Marianne Gingher (bio) Click for larger view View full resolution Pie Love You, 2014, illustrated by the author. In the beginning, he acted like a man who hadn’t eaten a home-cooked meal in twenty years. His first wife had been too unhappy to cook and the second one, too angry. He couldn’t get over my cooking. He didn’t cook, but he’d sit in the kitchen and watch me frying up chicken livers or making Lowcountry crab cakes out of a Charleston cookbook or rolling out a pie crust, and his handsome face wore the balmiest, most satisfied look that I’d ever seen on a man. I’d grown up among southern cooks: my maternal grandmother was from Kentucky and my paternal grandmother from North Carolina. They knew their way around the southern [End Page 110] kitchen, and I have their recipes to prove it—though I never saw either of them consult a cookbook. My Kentucky grandmother, Elizabeth, made a twelve-egg angel food cake from scratch that was as tender as fluff and always accompanied by cups of boiled vanilla custard, served chilled, that you dipped slices of the cake into. Her mother, my great-grandmother Mary Yeager, had taught her to churn butter, and Granny had much respect for butter. You never spread anything but on your toast or the yeast-risen bran rolls she baked when you visited her. Granny was a superb pie maker. Her signature dessert was a fairytale concoction called Coronation Butterscotch Pie made with caramelized sugar and slathered with turrets of meringue. She loved to tell the story about that pie saving her marriage. Despite the culinary delights that came out of her kitchen, Granny was a slow and dreamy cook, forgetful, easily distracted, and disorganized. During the Depression, after she and my grandfather lost their house and lived in a rented cracker-box, he located a German girl who, for room and board, helped Granny cook and clean. But Granny never mastered the art of domestic proficiency. Hosting family reunions, my grandfather always employed a cook to help prepare our evening meal and tidy up afterwards. The one who became our patron saint was a soft-spoken, gently efficient African American woman named Maggie. She worked shoulder-to-shoulder with Granny, and at 4 p.m., when they began their dinner preparations, the kitchen was off-limits to us children. Because of Maggie, Granny stayed on task and we ate on time. Many years later, after a disabling stroke, my mother became slow in the kitchen, too, but she did not want to give up cooking. For inspiration, she hung a picture of Maggie above the stove and carried on. My North Carolina grandmother, Ruth, a jackrabbit in the kitchen, made cakes more than she baked pies. She served a killer skillet cornbread and deep-fried her own hush puppies. Both my grandmothers fried chicken up a storm. In the summer Grandmother Ruth stirred up the sweetest succotash I’ve ever eaten, a toss of garden fresh baby lima beans and Silver Queen corn, the kernels as tiny and white as baby teeth with lots of fresh black pepper and just the right amount of bacon fat. We sprinkled vinegar and sugar on our sliced German Johnson tomatoes, and she made a watermelon rind pickle so luminous and translucent green it could have come from the Emerald City of Oz. My mother loved to bake pies, and my first cooking lesson, when I was ten or so, was learning how to make a piecrust. The only pie I didn’t try to bake was the Coronation Butterscotch pie. Its history intimidated me, but Mother was a whiz at it. Only after she stopped cooking did I give it a go, much to my sons’ delight, and for all the trouble it is to make, it’s become a holiday staple. Today, I’m a big fan of The Moosewood Cookbook, Laurie Colwin’s Home Cooking, The Silver Palate, and the many recipes offered by Melissa Clark and Mark Bittman that I’ve clipped from the New York Times. But...

In vitro antioxidant activity of extracts from common legumes

The in vitro antioxidant activity of pinto bean, cowpea, baby lima bean, lentil, chickpea, small red bean, red kidney bean, black kidney bean, navy bean, and mung bean extracts were investigated. Significant differences were observed in the phenolic content and the antioxidant activity amongst the legume extracts. Lentils demonstrated the highest phenolic content (47.6mg/g), total antioxidant activity (720.68U/g), DPPH• scavenging activity (38.5%), and total reducing power, whereas baby lima beans and navy beans had the lowest. Amongst the extracts, hydroxyl radicals (•OH) scavenging was higher in black kidney bean (85.68%) and baby lima bean (74.97%) extracts. The total antioxidant activity (r=0.84), DPPH• scavenging activity (r=0.83), and total reducing power (r=0.84) were positively correlated with the total phenolic content. However, •OH scavenging and the phenolic content were not correlated.

First Report of Downy Mildew of Lima Bean Caused by Phytophthora phaseoli in Virginia.

Delaware, the eastern shore of Maryland, and southern New Jersey have been the center of lima bean (Phaseolus lunatus L.) production in the eastern United States for nearly 50 years (1). Downy mildew has been the most important disease of lima bean in the humid eastern United States over that period. The causal agent of downy mildew, the oomycete pathogen Phytophthora phaseoli Thaxt., was first identified on lima bean in Connecticut in 1887 by Thaxter. Signs and symptoms of lima bean downy mildew include infection, necrosis and abscission of flowers, and shepherd's crooking of racemes, shoot tips, and petioles (1). Sporangia develop on shoot tips, petioles, pins (small pods), and pods in the field and on hypocotyls in-vitro. Since 2005, approximately 50% of the baby lima beans processed in the United States have been grown in Delaware and the eastern shore of Maryland. In 2008, commercial lima bean production began on the eastern shore of Virginia in Accomack County but no downy mildew was reported in that season. In 2009, approximately 1,825 ha in Accomack and Northampton counties were planted to baby lima bean. Weather conditions in 2009, including above average rainfall, were conducive for the development of downy mildew on the Delmarva Peninsula. Downy mildew was widespread in growers' fields in August and September in butter bean in southern New Jersey and baby lima bean in Sussex County, DE. In August 2009, a home gardener in Rappahannock, VA sent samples of infected lima bean pods from baby, Fordhook, and pole lima bean plants to the Virginia Tech Plant Disease Clinic in Blacksburg. On the basis of morphometric analysis, samples were determined microscopically to be infected by a Phytophthora sp. with rather uniform sporangia averaging 39 × 22 μm and short pedicels, diagnostic for P. phaseoli (1). On October 27, 2009, field scouts in Accomack County, VA identified two lima bean fields planted to cv. C-Elite-Select exhibiting moderate symptoms of downy mildew. Samples were brought to the Plant Diagnostic Clinic at the University of Delaware under USDA-APHIS permit and determined to be P. phaseoli based on morphometric analysis. Samples were inoculated onto a lima bean cultivar differential to determine pathogenicity to complete Koch's postulates and to determine their physiological race. Samples were inoculated onto lima bean cvs. 184-185 and C-Elite-Select, which are susceptible to race F and resistant to race E, Eastland and 8-78, which are susceptible to race E and resistant to race F, and Concentrated Fordhook, susceptible to all known races (1). Three pots containing five emerging seedlings each were inoculated with sporangia (approximately 103 per ml) prepared by soaking infected pods in 500 ml of sterile distilled water for 1 min with gentle agitation. Plants were placed in a Percival dew chamber with intermittent misting and set at 19. Infection and disease development were assessed daily and signs developed 7 days postinoculation in cvs. 184-85, C-Elite-Select, and Concentrated Fordhook, but not in Eastland and 8-78. Cultivar differential tests indicated that the isolates were P. phaseoli race F. Hypocotyls of infected plants were scraped, and isolations made on lima bean pod agar confirmed the presence of P. phaseoli. To our knowledge, this is the first time that downy mildew of lima bean has been reported in Virginia. Reference: (1) T.A. Evans et al. Plant Dis. 91:128, 2007.

Effects of Baby Lima Bean (Phaseolus Lunatus) Flour as a Partial Substitute for all Purpose Flour on the Sensory and Objective Characteristics of Muffins

Effects of Baby Lima Bean (Phaseolus Lunatus) Flour as a Partial Substitute for all Purpose Flour on the Sensory and Objective Characteristics of Muffins

An antifungal peptide from baby lima bean

A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with an IC(50) of 4 muM. The peptide exhibited an N-terminal amino acid sequence similar to those of leguminous defensins. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl (DEAE)-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on carboxymethyl (CM)-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The peptide was unadsorbed on DEAE-cellulose and Affi-gel blue gel but was adsorbed on CM-cellulose.

The 'Maffei 15' Lima Bean Compensates for Reduced Plant Stand

'Maffei 15' baby lima bean seeds were sown every 6 cm in rows 76 cm apart to yield a nominal stand of 215,000 plants/ha at two locations in Delaware over 2 years. Seedlings were thinned within 2 weeks of planting to provide 0%, 16.7%, 33.3%, and 50.0% stand reduction at two in-row spacing patterns to determine subsequent effects on vegetative and reproductive growth. Shoot fresh weight per square meter was decreased only in 2003 by 21% and bean fresh weight per square meter was decreased only in 2004 by 13.8% when plant stand decreased to 50%. This disproportional vegetative and reproductive growth response to stand reduction resulted from a compensatory linear increase in shoot fresh weight, usable pod number, and bean fresh weight of individual plants. Thus, 'Maffei 15' lima bean tolerates a considerable loss of plant stand with little or no effect on yield.

A Precision Planter for Large Seeds in Small Plots

A mechanical planter was developed to sow seed of baby lima beans (Phaseolus lunatus) in small plots. The mechanical seeder allowed small plots to be quickly and consistently seeded at a fixed spacing. Seeds were manually spread along a 10-ft (3.0 m) base plate containing 50 holes of slightly larger diameter than the seed length and at the desired seed spacing [2.4 inches (6 cm)]. Once all the holes were filled, a slider plate below the base plate containing holes of the same diameter and spacing, but which were slightly offset, was slid horizontally so that the holes of the base and slider plates aligned and the seeds dropped to the bottom of the furrow. Compared to manual planting, the mechanical planter increased the precision of seed placement and reduced the time needed to plant 50 seeds. The planter was easy to use and transport, and was inexpensive.

First Report of Phytophthora capsici Infecting Lima Bean (Phaseolus lunatus) in the Mid-Atlantic Region.

Lima beans are an important crop in Delaware and the Mid-Atlantic Region. In the summer of 2000, five commercial cultivars (3-28, 184-85, C-elite Sel, Butter Bean, and Jackson Wonder) of lima bean in Delaware, Maryland, and New Jersey were observed with white, appressed mycelia on infected pods that appeared distinctly different from signs of downy mildew infection caused by Phytophthora phaseoli. Isolations were made by placing diseased pods between layers of rye media (1). A fungus that produced white mycelia with sporangia was consistently isolated. All Phytophthora isolates from the infected pods were heterothallic, grew at 35°C, had as much as 100 μm long pedicles on varying shapes of caducous sporangia with tapering base and >2 papillae, and were identified as P. capsici (2). Initially, three surface-disinfected pods from cv. Early Thorogreen plants grown in the greenhouse were floated on 20 ml of sterile water in a petri dish, and each was inoculated with a disk of P. capsici. This was repeated for nine isolates obtained from lima bean. After incubation for 7 days at room temperature, all 27 pods were infected, and P. capsici was reisolated from all the pods. A pathogenicity test was performed on the same cultivars from which the original field isolates were collected. Three seedlings and two plants with mature pods were inoculated with a sporangial suspension of each of the nine isolates and placed in a dew chamber for 5 days at 20 to 25°C and 100% relative humidity. White mycelial growth was observed on seedlings and mature pods. One inoculated plant developed brown-to-black stem lesions with white mycelia. All pods on the mature plants showed appressed, white mycelia identical to that observed in the commercial lima bean fields. P. capsici was consistently reisolated from all inoculated plants. In 2000, most infected pods in infested fields were observed low in the plant canopy or touching the soil. However, in 2001, infected pods were mostly in the lower and mid-portion of the plants observed in baby lima bean fields in Kent County, DE.

Physicochemical and Functional Characterization of Baby Lima Bean(Phaseolus lunatus) Starch

One potential source of starch is the tropical legume baby lima bean (Phaseolus lunatus) that contains around 56—60% of starch. The objectives of this work were to evaluate this starch's physicochemical and functional properties and compare it with the properties of other starches. The chemical composition of lima bean starch was: 10.16% moisture, 0.20% protein, 0.67% fiber, 0.14% ash, 0.54% fat, 98.43% starch and 0.013% phosphorus. The amylose content was higher (32.7%) than that of other cereal and tuber starches but similar to other legume starches. The average granule size (diameter 17.9 μm) was comparable to that of corn starch and of other legume starches. The granule was heterogeneous, presenting an oval shape. The gelatinization temperature was 80.16 °C (range 75—87 °CC), which is similar to other legume starches but higher than that of corn starch. The molecular size (alkali number 3.22), was smaller than that of potato starch but similar to that of corn starch. Compared to corn starch, the gels were firmer and presented a higher degree of retrogradation even at high concentrations. The water solubility was positively correlated with the temperature: i.e., 1.8, 3.4, 8.5 and 12.3% at 60, 70, 80 and 90 °C, respectively. The swelling power had the same behaviour: 2.6, 3.3,12.8 and 19.9 g of water/per gram of starch at 60, 70, 80 and 90 °C, respectively. The amylogram showed that the viscosity (680 Brabender units) and stability were higher than those of commercial corn starch (252 Brabender units). The use of this starch in the preparation of syrups with high glucose contents, as well as in baked and canned products that require heating, is suggested.

A Comparison of Production Practices and Fruit Development for ‘M-15’ Lima Beans Grown in Delaware and California

ABSTRACT Average yields of ‘M-15’ baby lima beans (Phaseotus lunatus L.) are consistently lower in Delaware (1,905 kg/hectare) than in California (3,923 kg/hectare). Comparison of production practices revealed differences in plant populations, soil type, irrigation, relative humidity, and plant size for the two areas. Documentation of lima bean ontogeny revealed more than one flower cluster at a single node in an inflorescence can be produced. Although multiple flower clusters can be produced, pods at harvest were the products of initial flower clusters. Inflorescence location, above or within the plant canopy, did not affect yield per inflorescence. Consistent with commercial yields, ‘M-15’ research plots in California had greater yields than those in Delaware in Experiment I. In Experiment II, ‘M-15’ was grown at the same plant population density in both locations. Again, California had significant increases in plant mass and shelled bean masses over those planted in Delaware.

Tryptic fragments of phaseolin from protein isolates of Phaseolus beans

Crystalline and amorphous protein isolates from white kidney and navy beans (Phaseolus vulgaris) and baby lima and large lima beans (Phaseolus lunatus) were subjected to tryptic hydrolysis. The hydrolysis was monitored by the nitrogen solubility index (NSI) and reverse phase-high performance liquid chromatography/mass spectrometry (RP-HPLC/MS) analysis of the hydrolysates. The results of the NSI and RP-HPLC showed that the tryptic fragments of the crystalline isolates possessed higher solubility properties than the fragments of the amorphous isolates. The results of the MS study of phaseolin peptides in the RP-HPLC fractions of the hydrolysates showed that trypsin-specific bonds located in β-structured regions of β-phaseolin are resistant to tryptic hydrolysis, and that the most susceptible bonds to trypsin hydrolysis are located in regions not directly involved in the formation of secondary structural units, but in regions of disordered structure, or in regions interconnecting secondary structural units. Some trypsin-specific bonds located within α-helical structures were also cleaved by trypsin hydrolysis.