B12 Content Research Articles

Phenolic compounds in grape seed extract inhibiting the formation of benzo[a]pyrene in baked bacon related to scavenging free radicals and attenuating Maillard reaction

Grape seed extract (GSE) is a known natural antioxidant, but the inhibitory effect and mechanism of GSE on benzo(a)pyrene (BaP) in cured meats have not been investigated. This work investigated the effects of two major phenolic compounds in GSE, namely proanthocyanidin B2 and isorhamnetin, on the formation of BaP, free radical scavenging ability, color value, 5-hydroxymethylfurfural (HMF) level and Amadori compound content. The results showed that proanthocyanidin B2 and isorhamnetin significantly inhibited BaP production in bacon (P < 0.05). The inhibition of BaP by 0.003% concentration of procyanidin B2 or isorhamnetin was 65.43% and 52.66%, respectively. Adding these phenolic compounds to bacon significantly reduced b* color value, HMF content, and Amadori compounds. Subsequently, the mechanism of BaP inhibition by these phenolic compounds was further systematically elucidated by modeling the glucose/lysine reaction, which was instrumental in scavenging free radicals and inhibiting the Maillard reaction. These findings provided valuable insights into the mechanism of BaP reduction facilitated by GSE, and propose the potential utility of GSE phenolics to mitigate BaP levels in baked bacon.

Hermetia illucens Fermented with Lactobacillus plantarum KCCM12757P Alleviates Dextran Sodium Sulfate-Induced Colitis in Mice.

Inflammatory bowel disease (IBD) can severely affect humans and animals and is difficult to treat. Black soldier fly (Hermetia illucens; Hi) larvae (BSFL) are a sustainable source of protein. However, no studies exist on the antioxidant and anti-inflammatory functions of BSFL or fermented BSFL with respect to IBD. In this study, riboflavin-producing Lactobacillus plantarum KCCM12757P was isolated from a fish farm tank, and in conjunction with hot water-extracted Hi (HeHi) (termed HeHi_Lp), was used to determine optimal fermentation conditions to increase vitamin B2 concentration. This in vivo study investigated the therapeutic effects and mechanistic role of HeHi_Lp in chronic colitis-induced murine models. Histological changes, inflammatory cytokine levels, and intestinal barrier function were explored. Gut microbial communities and gene expression in the nuclear factor (NF)-κB signaling pathway were also studied. HeHi_Lp remarkably reduced the disease activity index, inflammatory cytokine (inducible nitric oxide synthase, cyclooxygenase 2, tumor necrosis factor α, interleukin (IL-6 and IL-1β) levels, and increased body weight and colon length. HeHi_Lp administration significantly raised zonula occludens 1, occludin and claudin 1 and improved the composition of the gut microbiota and beneficial intestinal bacteria. These results suggest that HeHi_Lp can be used as a dietary supplement in pet food to alleviate colitis.

Investigation of Chemical Composition and Proximate Properties of Bush Mango (Irvingia wombulu) Production Management and Ethno-medicinal Benefits of Rural Dwellers

The chemical analyses of bush mango (Irvingia wombulu) was conducted in order to identify the bioactive content, proximate composition as well as to establish the vitamin composition in bush mango production. The extraction processes were carried out with the aid of different solvents and techniques. The analyses was conducted with aqueous extract of the milled seed of Irvingia wombulu which found the bioactive components present in form of tannins, saponin, flavonoids, steroid, protiens, anthraquinnones, protein, reducing sugar, cardiac glycoside, terpenopid, phenolic compounds and alkaloids. Flavonoid, cardiac glycoside andalkaloids were moderately present (+) while tannis, saponins, steroids, protein and reducing sugar were heavily present(++). Further, the results from the analyses displayed the proximate composition in Irvingia wombulu leaves, peels, seeds and seed coats samples. The moisture contents in seeds, leaves, peels and seed coats of Irvingia wombulu were 1.4±0.41%, 22.2±0.11%, 38.7±0.01%, and 57.6±0.90% respectively. The test found that the mineral contents of Irvingia wombulu in the seed coats had the highest concentration of iron (0.395±0.00 mg/kg) and 0.040±0.02mg/kg was found in the seed. The trend in the level of concentration of sodium in I. wombulu revealed that leaves has more sodium than seeds and seeds more than seed coats, peels is the least. For calcium, the analyses revealed that the level of concentration in the seed coats had higher mineral content of 4.992±0.01 mg/kg as compared to the lower level of concentration in the seeds at 3.278±0.01 mg/kg. The analyses further showed vitamin B2 composition known riboflavin as one of the 8 -B vitamins. The value of vitamin B2 was high in Irvingia wombolu at 0.17mg/100g with vitamin B6 content having 0.14gm/100g. The production of these chemical components would contribute to health benefits of the rural dwellers. The high bioactive content potential and the presence of proximates, mineral content, vitamins produced at different levels in bush mango (Irvingia wombulu) should be utilized to develop drugs and supplements for its ethno-medicinal benefits and to generate internal revenue for the FRIN biomedical department. Keywords: Bioactive Components, Metabolites, Phytonutrients, Vitamins, Milled Kernel, Production

Mycotoxin reduction and metabolite profiles of ogi produced using traditional fermentation methods

Mycotoxins are widely present in maize, a favourite staple food in sub-Saharan Africa. Food processing methods, like fermentation, have been suggested as potential ways to reduce mycotoxin contamination levels in the grain and, as a result, limit the exposure of crop consumers to the harmful effects of the toxins. The influence of four traditional fermentation processes [cold (with changed steeping liquor (CSL) and unchanged steeping liquor (USL), Fon and Goun procedures] on the mycotoxin reduction and metabolites profile of ogi, a fermented maize product, was studied. Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) and gas chromatography linked to high resolution time-of-flight mass spectrometry (GC-HR-TOF-MS) were respectively employed for the mycotoxin and metabolite profiles analyses of the samples. Among the nine mycotoxins detected in the raw maize samples, aflatoxin B1 (AFB1) and fumonisin B1 (FB1) + fumonisin B2 (FB2) concentrations were found to exceed the European Union (EU) maximum limit. Both cold (containing USL and CSL) and Goun fermentation techniques were able to lower the AFB1 concentration below this threshold. The metabolomics result revealed that ogi produced using the cold (USL) and Fon fermentation processes had the highest number of most of the detected important compounds, whereas the Goun fermentation process produced the fewest compounds in total. There was no statistically significant difference in the ability of the specified natural fermentation processes to lower FB1, FB2, FB3, deoxynivalenol (DON), sterigmatocystin (STERIG), and zearalenone concentrations in maize (ZEN). In addition, the results demonstrated that the four natural fermentation processes evaluated had varying effects.

Application of multi-omic features clustering and pathway enrichment to clarify the impact of vitamin B2 supplementation on broiler caeca microbiome.

The results of omic methodologies are often reported as separate datasets. In this study we applied for the first time multi-omic features clustering and pathway enrichment to clarify the biological impact of vitamin B2 supplementation on broiler caeca microbiome. The caeca contents of broilers fed +50 and +100 mg/kg vitamin B2 were analyzed by shotgun metagenomic and metabolomic. Latent variables extracted from NMR spectra, as well as taxonomic and functional features profiled from metagenomes, were integrated to characterize the effect of vitamin B2 in modulating caeca microbiome. A pathway-based network was obtained by mapping the observed input genes and compounds, highlighting connected strands of metabolic ways through pathway-enrichment analysis. At day 14, the taxonomic, functional and metabolomic features in the caeca of tested broilers showed some degree of separation between control and treated groups, becoming fully clear at 28 days and persisting up to 42 days. In the caeca of birds belonging to the control group Alistipes spp. was the signature species, while the signature species in the caeca of broilers fed +50 and +100 mg/kg vitamin B2 were Bacteroides fragilis and Lactobacillus crispatus, Lactobacillus reuteri, Ruminococcus torques, Subdoligranum spp., respectively. The pathway enrichment analysis highlighted that the specific biochemical pathways enhanced by the supplementations of vitamin B2 were N-Formyl-L-aspartate amidohydrolase, producing Aspartate and Formate; L-Alanine:2-oxoglutarate amino transferase, supporting the conversion of L-Alanine and 2-Oxoglutarate in Pyruvate and L-Glutamate; 1D-myo-inositol 1/4 phosphate phosphohydrolase, converting Inositol 1/4-phosphate and water in myo-Inositol and Orthophosphate. The results of this study demonstrated that the caeca of birds fed +50 and + 100 mg/kg were those characterized by taxonomic groups more beneficial to the host and with a higher concentration of myo-inositol, formic acid, amino acids and pyruvate involved in glycolysis and amino acid biosynthesis. In this study we demonstrated how to perform multi-omic features integration to describe the biochemical mechanisms enhanced by the supplementation of different concentrations of vitamin B2 in the poultry diet. The relationship between vitamin B2 supplementation and myo-inositol production was highlighted in our study for the first time.

Kombucha Fermentation of Olympus Mountain Tea (Sideritis scardica) Sweetened with Thyme Honey: Physicochemical Analysis and Evaluation of Functional Properties.

This study implemented kombucha fermentation of Olympus Mountain tea (Sideritis scardica) sweetened with honey (OMTWH) in order to investigate the potential for producing a novel beverage with functional properties. The increase in the total count of bacteria and yeast suggests that the OMTWH acts as a viable substrate for supporting the proliferation of the microorganisms of the Kombucha symbiotic culture. The fermentation resulted in a reduction in pH and increased total titratable acidity. After fermentation, a statistically significant increase in the vitamins C, B1, B2, B6, B7, and B12 content was observed (p < 0.05). Total phenolics and antioxidant activity of the fermented beverage was significantly enhanced, as assessed by the method of Folin-Ciocalteu and ABTS assay, respectively. Results revealed that OMTWH had a potent inhibitory activity of α-amylase, α-glucosidase, acetylcholinesterase, and butyrylcholinesterase; OMTWH fermented with a kombucha consortium exhibited even higher inhibition. Hence, the process of kombucha fermentation can convert OMTWH into a novel beverage with enhanced functional properties.

Epiphytic microflora and mycotoxin content in meadows-Is plant biodiversity affecting fungal contamination?

Ecosystem services are an important aspect of grasslands utilization; however, they are often contradictory to their main purpose, which is a production of good quality and safe feed. In this study, we evaluated the difference between grass monocultures and species-rich mixtures in terms of epiphytic microflora and mycotoxin contamination levels. We hypothesized that higher species diversity would lead to higher microbial counts, which could lead to higher mycotoxin contamination risk. Differences in epiphytic fungal, yeast and total amount of microorganisms (CFU g -1) depending on the species diversity in the field has been evaluated by cultivation method. Concentration of deoxynivalenol (DON), zearalenone (ZEN) and aflatoxin B1 (AFB1) was measured by ELISA. Results are suggesting that higher total amount of microorganisms were found in monocultures, however, fungal and yeast counts were higher in species-rich mixtures. Higher species diversity of grasses was related to higher total microbial count (TMC) and yeast colonization of phyllosphere. Our results suggest higher risk of fungal phyllosphere colonization of species-rich mixtures with higher biodiversity and therefore higher risk of mycotoxin contamination of such feed.

Terahertz toroidal dipole metamaterial sensors for detection of aflatoxin B1

Terahertz metamaterial biosensors have attracted significant attention in the biological field due to their advantages of label-free, real-time and in situ detection. In this paper, a highly sensitive metamaterial sensor with semi-ring mirror symmetry based on toroidal dipole resonance is designed for a new metamaterial biosensor. It is shown that a refractive index sensitivity of 337.5 GHz per refractive index unit can be achieved under an analyte of saturated thickness near a 1.33 THz transmission dip. For biosensor samples where aflatoxin B1 is dropped on the metamaterial surface in our experiment, dip amplitudes of transmission varying from 0.1904 to 0.203 and 0.2093 are observed as aflatoxin B1 concentrations are altered from 0 to 0.001 μg ⋅ml−1 and to 0.01 μg ⋅ml−1, respectively. Furthermore, when aflatoxin B1 concentrations are 0.1 μg ⋅ml−1, 1 μg ⋅ml−1, 10 μg ⋅ml−1 and 100 μg ⋅ml−1, dip amplitudes of 0.2179, 0.226, 0.2384 and 0.2527 and dip redshifts of 10.1 GHz, 20.1 GHz, 27.7 GHz and 37.6 GHz are respectively observed. These results illustrate high-sensitivity, label-free detection of aflatoxin B1, enriching the applications of sensors in the terahertz domain.

The influence of a maternal vegan diet on carnitine and vitamin B2 concentrations in human milk.

The maternal diet greatly influences the nutritional composition of human milk. With the rise of vegan diets by lactating mothers, there are concerns about the nutritional adequacy of their milk. Two important nutrients, vitamin B2 and carnitine, are mostly ingested via animal products. We investigated the influence of a vegan diet on the vitamin B2 and carnitine concentrations in milk and serum of lactating women. In this case-control study, 25 lactating mothers following an exclusive vegan diet were comparted to 25 healthy lactating mothers with an omnivorous diet without use of supplements. High-performance liquid chromatography and liquid chromatography-tandem mass spectrometry were used to measure vitamin B2 and carnitine concentrations, respectively. A linear regression model was used to determine differences in human milk and serum concentrations between study groups. Vitamin B2 concentrations in human milk and serum did not differ between study groups. While the human milk free carnitine (C0) and acetyl carnitine (C2) concentrations did not differ between study groups, serum carnitine concentrations were lower in participants following a vegan diet than in omnivorous women (p < 0.0001). A maternal vegan diet did not affect human milk concentration of vitamin B2 and carnitine. Breastfed infants of mothers following an exclusive vegan diet therefore are likely not at increased risk of developing a vitamin B2 or carnitine deficiency.

Testing the Efficiency of Silver Nanoparticles Manufactured Locally by the Alga Spirogyra in Inhibiting the Fungus Aspergillus flavus and Reduction of Aflatoxin B1

This experiment was conducted in the mycotoxins lab at the College of Agricultural Engineering Sciences-University of Baghdad with the aim of evaluating the effectiveness of bio- and locally manufactured silver nanoparticles by the alga Spirogyra locally and biologically manufactured silver nanoparticles in inhibiting the fungus Aspergillus flavus and reduction of aflatoxin B1. The results showed the alga Spirogyra highly efficient in the biologically manufactured silver nanoparticles and the size of the synthesized nanoparticles was 8.504 nm when examined by atomic force microscope AFM, which showed a high efficiency in inhibiting the isolate of the fungus Aspergillus flavus at concentrations 1.5, 2 and 3, and the percentage of inhibition was 86.7%, 97.64, 100%, respectively, It also indicated a high efficiency in preventing the fungus from producing aflatoxin B1 on the stored wheat grains. The concentrations of toxins in stored wheat samples were 6.25 ppb, 4.11 ppb and 0.0ppb for concentrations 1.5, 2, and 3 compared with the control treatment in which the concentration of aflatoxin B1 was 88.4ppb, as the percentage of reduction was 92.92%, 95.35% and 100, respectively. The results of biologically manufactured silver nanoparticles also showed an efficiency in reducing aflatoxin B1 from stored wheat grains. The concentration (3) was superior in reducing aflatoxin B1 to 2ppb compared to concentrations of (1.5 and 2), in which the toxin concentration reached 10.25 ppb and 7.15 ppb, respectively, with a reduction percentage of 88.4%, 91.91% and 97.73%.

Prevalence of aflatoxin along processing points of locally made complementary food formulae in northern Uganda: Safety and children's exposure across seasons

Aflatoxin contamination along the processing points of locally made complementary food composite needs to be ascertained and minimized to reduce exposure to weaning children. The study established the concentrations of total aflatoxin (TAF) and aflatoxin B1 (AFB1) along the processing points of locally made malted millet sesame soybean composite (MMSSC) across season one (wet) and season two (dry) and determined children's exposure to them. A total of 363 samples were collected in 2019. TAF and AFB1 concentrations were determined quantitatively using an enzyme-linked immunosorbent assay (ELISA). Consequently, exposure of individual children was assessed as Estimated Daily Intake (EDI), (ng kg−1 bw day−1). All the samples along the processing points had detectable concentrations of TAF and AFB1 ranging from 0.578 μg kg−1 to 1.187 μg kg−1 and 0.221 μg kg−1 to 0.649 μg kg−1 respectively. Contamination was highest in raw materials; soybean (Glycine max) > sesame (Sesamum indicum), followed by stored composite, freshly prepared composite, and least in millet (Eleusine coracana). Contamination varied significantly across seasons with the wet season having higher contamination than the dry season at P = 0.05. All samples (100%) were within the European Commission (EC) acceptable maximum tolerable level for TAF and AFB1 (4 μg kg−1 and 2 μg kg−1) respectively for processed foods for general consumption. But were below the EU acceptable maximum tolerable level for TAF and AFB1 (0.4 μg kg−1 and 0.1 μg kg−1) respectively for processed baby foods cereals. However, all were within the United States- Food and Drug Authority (US-FDA) and East African Community (EAC) set maximum acceptable limit of 20 μg kg−1 for TAFs, 10 μg kg−1 and 5 μg kg−1 for TAF and AFB1 respectively. Conversely, exposure to these toxins was much higher than the Provisional Maximum Tolerable Dietary Intake (PMTDI) of 0.4 ng kg−1 bw day−1 to 1.0 ng kg−1 bw day−1. A significant difference in exposure to both toxins was observed with the weight. The age of 5 months was the most exposed. A concerted effort is needed to reduce children's exposure to MMSSC to TAF and AFB1, taking sesame and soybean as priority ingredients and proper storage based on season to control contamination.

Complex Evaluation of Storage Impact on Maize (Zea mays L.) Quality Using Chromatographic Methods

Maize (Zea mays L.), a food crop cultivated worldwide, is renowned for its nutritional and economic value. However, its quality can be significantly affected by various storage conditions, leading to changes in its nutritional composition and potential contamination with harmful substances, such as aflatoxins. This article presents a comprehensive evaluation of the impact of storage on maize quality, focusing on several key parameters analyzed using liquid chromatographic methods and FT-NIR spectrometry. The research reveals a decline in the concentrations of key nutrients over the storage period. Specifically, there was a 19.7% loss in vitamin B1, a 12.43% loss in vitamin B3, a 16.96% loss in α-tocopherol, a 13.61% loss in total tocopherols, and an 8.02% loss in β-carotene. Aflatoxins were mostly undetectable, with one exception in January that remained below the maximum permitted level of 0.3 µg/kg. The parameters include the concentration of vitamins B1, B3, α-tocopherol, total tocopherols, β-carotene, aflatoxin B1, total aflatoxins, carbohydrate content, and protein content. Aflatoxins were mostly undetectable, with one exception in January—0.3 µg/kg—that remained below the maximum permitted level. The study underscores the importance of cooling maize immediately after drying to minimize nutrient loss, providing valuable insights for optimizing storage processes to maintain the nutritional quality of maize.

B vitamin quantification in lentil seed tissues using ultra-performance liquid chromatography-selected reaction monitoring mass spectrometry

Lentils are a nutritious food in the human diet. High in protein and with low glycemic index, lentils are also a source of folate and other B vitamins. Understanding variability in B vitamin contents among lentils will allow breeders to select for increased levels. We analyzed 34 cultivated and three wild genotypes for vitamins B1, B2, B3, B5, B6, B7, and B9 in the cotyledons and seed coats. Variation for all B vitamins was observed across the genotypes. Cotyledons had higher concentrations of B1 and B3, while seed coats had higher concentrations of B2, B5, B6, and B9. Wild accessions had the highest concentrations of vitamin B9 and were among the highest for B2. Differential distribution of B vitamins across seed tissues and lentil genotypes has implications for consumption and for breeding. There is useful genetic variability which could be used to increase B vitamin levels in future lentil varieties.

Optimization of vitamin B12 nano-emulsification and encapsulation using spontaneous emulsification.

In this study, the use of low-energy methods for nanoemulsification of vitamin B12 was investigated to protect this bioactive substance. The effects of sunflower oil concentrations (4-8%), Tween 80 (8-16%), and vitamin B12 (5-15%) on the physicochemical properties of B12 nanoemulsion were evaluated using response surface methodology (RSM). The results indicated that the quadratic model was the most fitting model for experimental data. Optimization revealed that the optimal formulation contained 6.5% sunflower oil, 9.6% Tween 80, and 13% vitamin B12, resulting in maximum efficiency, viscosity, and vitamin B12 content, as well as minimum pH, turbidity, p-Anisidine index, particle size, and polydispersity index (PDI). Under optimal conditions, pH, viscosity, turbidity, efficiency, vitamin B12, p-Anisidine index, PDI, and particle size were 7.24, 17.0024 cp, 2.19, 51.98%, 5.54ppm, 0.01, 0.34, and 322nm, respectively. This study highlights the effectiveness of spontaneous emulsification as a carrier for the encapsulation of bioactive compounds.

The Effect of Immersing the Bud Cuttings with a Combination of NAA and BA Growth Regulators and the Addition of Optimus Plus Nanofertilizer in Improving some Vegetative Qualities of Ber Seedlings Apple Variety

The study was carried out during the growing season 2022 in one of the orchards located in Al-Sajla area / Al-Khairat district / Holy Karbala governorate On seed Sidr varieties two years old. To find out the effect of immersing bait pens, an apple cultivar was treated with a combination of growth regulators in three concentrations (0, 40 + 20, 40 + 80 mg.L-1) and symbol (A0, A1, A2) in sequence, and the addition of Optimus Pius nano fertilizer in three concentrations (0, 20, 40 m. L-1) and its symbol (B0, B1, B2) sequentially and the overlap between them in the vegetative traits of Sidr seedlings, apple cultivar. The experiment was carried out according to the randomized complete block design (RCBD) as a factorial experiment with three replications. The results showed that immersing the grafting pen with a combination of growth regulators at A1 concentration had a significant effect on vegetative traits (percentage of vaccination success, main stem length, main stem diameter, number of lateral branches, number of leaves and leaf area) amounted to (88.900%, 142.567 cm, 40.047 mm 17.447 branch. Sapling, 827 leaf. Sapling, 22.567 cm2. sapling-1) sequentially compared to the comparison treatment, Ground fertilization with nano-fertilizer at a concentration of B2 also led to a significant increase in (percentage of vaccination success, main stem length, main stem diameter, number of lateral branches, number of leaves and leaf area) amounting to (88.900%, 144.900 cm, 40.150 mm, 17.667 branch. Sapling, 828 leaves. Seedling-1, 22.967 cm2. sapling-1) sequentially compared with the comparison treatment. The bilateral interaction between the two study factors also led to a significant effect on all vegetative characteristics of Sidr seedlings of an apple variety, especially when treated (A1 B2) compared to the comparison treatment of the overlap.

Estimation of Caffeine and Vitamin B-Complex (Vitamin B2, B3, B5 and B6) Constituents of Selected Energy Drinks

Vitamins are important nutrients that are contained in food and carry out distinct and important bodily tasks that are required for sustaining good health. A xanthine alkaloid known as caffeine is widely distributed in natural plant sources such as coffee, cocoa beans, cola nuts, and tea leaves. Additionally, it is a raw material in several drinks. Its usage increases blood pressure, diuresis, blood sugar, generation of stomach acid and pepsin, plasma levels of fatty acids, cortisol, and epinephrine, intraocular pressure, and calcium loss, which causes bone loss. The phrase "energy drinks" refers to beverages that advertise that they will give you an energy boost, which has led to an increase in the popularity of "energy drinks" that include caffeine. The caffeine and vitamin B-complex concentrations of a few different energy drinks were tested and compared in this study project. Samples of ten (10) different energy drink brands were chosen at random from Nkwo Ogbe Market in Ihiala, and their caffeine and vitamin B1, B2, B6, and B12 concentrations were determined using a spectrophotometric technique. The findings revealed that caffeine concentrations varied from 1.11 mg/L to 2.13 mg/L, and that vitamin B1 in energy drinks had the lowest value (0.014 mg/kg). All samples of energy drinks had caffeine levels below the 400 mg/L benchmark set by the National Agency for Food and Drug Administration and Control (NAFDAC). In order to avoid negative consequences, it is important to monitor any decrease in intake of these beverages because other criteria, such as aspartame and sugar, were also often below the established levels.

Traditional Asian plant‐based fermented foods as vitamin B12 sources: A mini‐review

AbstractPlant‐based foods, such as vegetables and beans, are deficient in vitamin B12 (B12). If plant‐based foods containing adequate B12 can be produced microbially, incorporating them into a vegetarian diet would prevent B12 deficiency. Asian indigenous fermented foods from soybeans, vegetables, and tea leaves, are commercially accessible. The B12 level in the fermented products has been characterized to determine whether they may serve as a viable source of B12 in humans. Since their B12 content is very low (&lt;0.5 μg/100 g wet weight), most traditional Asian plant‐based fermented foods do not appear to serve as adequate daily B12 sources. However, some stinky tofu and pickled vegetable products contain &gt;10 μg of B12 per 100 g wet weight. Nonetheless, further clinical research is warranted to clarify whether these products can increase the serum B12 levels of humans.

High-flux Hemodialysis Does Not Increase Vitamin B Loss Compared With Low-flux Hemodialysis

A consistent effect of hemodialysis (HD) on vitamin B loss has not been fully demonstrated and the effect of high-flux hemodialysis (HFHD) is also inconclusive. The aim of this study was to identify the loss of vitamin B1, B3, B5, and B6 in a single HD session and to evaluate the effect of HFHD on vitamin B removal. Patients on maintenance HD were enrolled in this study. They were divided into low-flux hemodialysis (LFHD) group and HFHD group. Vitamin B1, B3, B5, and B6 (pyridoxal 5'-phosphate [PLP]) concentrations in blood pre- and post-HD sessions, as well as in the spent dialysate were measured. Loss of vitamin B was calculated and the difference in vitamin B loss between the 2 groups was compared. The association between HFHD and vitamin B loss was estimated using multivariable linear regression analysis. Seventy-six patients were included, of whom 29 were on LFHD and 47 were on HFHD. The median reduction ratio of serum vitamins B1, B3, B5, and B6 after a single HD session was 38.1%, 24.9%, 48.4%, and 44.7%, respectively. The median concentration of vitamins B1, B3, B5, and B6 in the dialysate was 0.3μg/L, 2.9μg/mL, 2.0μg/L, and 0.4ng/mL. There was no difference in either the reduction ratio of vitamin B in blood, or the concentration in dialysate between LFHD and HFHD groups. After adjusting for covariates by multivariable regression, HFHD had no effect on vitamin B1, B3, B5, or B6 removal. Vitamins B1, B3, B5, and B6 can be removed by HD and HFHD does not increase the loss.

Abstract A061: Nuclear lamin content as a biomarker for prostate cancer progression

Abstract Lamins are proteins that help to structure the cellular nucleus via the formation of an intermediate filament weave that grants rigidity to the nuclear membrane. Lamins also tether the chromatin to the nuclear envelope, contributing to the formation of heterochromatin, necessary for adequate gene expression control. The purpose of this study was to use a combination of immunostaining, ChIP-seq, and chromosome conformation capture (Hi-C) to a) characterize the expression and deposition patterns of Lamin A, Lamin B1, and Lamin B2 in cell lines and patient samples, b) to evaluate chromatin associations with the lamina and, c) to characterize chromatin organization phenomena related to lamina conformation. In this study, we used a collection of cells that model the continuum of progression in vitro as follows: RWPE (normal prostate epithelium), LNCaP (adenocarcinoma), VCaP and MDAPCa2a/b (bone metastatic). We also evaluated whether the phenotypes for lamin deposition observed in vitro correlate with that of patient samples, via immunohistochemistry of tissue microarrays. We have identified that Lamin A content is significantly lower in bone metastatic cell lines. While VCaP cells lack Lamin A deposition, MDAPCa2a cells have a heterogeneous distribution: cells that grow in contact with the cell culture surface express Lamin A while cells that grow atop 3D clusters of cells do not. In contrast, Lamin B1 and B2 content is consistent across cell lines. Analysis of tissue microarray immunohistochemistry showed congruent results to the in vitro findings: patient samples derived from higher-grade adenocarcinomas show a decreased number of lamin A positive cells.​ This endogenous lack of Lamin A in VCaP results in an altered lamin-associated domain distribution that can potentially result in abnormal heterochromatin formation, as characterized by ChIP-seq. Further,​through a comprehensive analysis of genome architecture via chromosome conformation capture, we observe a change in the radial positioning of the chromosomes when comparing cells that model normal epithelium, adenocarcinoma, and bone metastasis. Given that the primary metastatic site for prostate cancer is the bone, we evaluated the effect of bioavailable calcium on lamin expression in vitro. Interestingly, we find that in cells cultured on vessels coated in amorphous calcium phosphate, lamin expression is ablated both in normal epithelium and adenocarcinoma cell lines, independent of testosterone levels. Taken together, our results show that lamin content, and in particular loss of lamin A, could be an early predictor of disease progression potential, affecting nucleus mechanics and pliability, ultimately facilitating metastasis. Further, the altered chromatin organization dynamics can potentially act as a prelude for pro-metastatic transcription Citation Format: Rebeca San Martin, Priyojit Das, Sanaz Hossain, Rachel Patton McCord. Nuclear lamin content as a biomarker for prostate cancer progression [abstract]. In: Proceedings of the AACR Special Conference: Advances in Prostate Cancer Research; 2023 Mar 15-18; Denver, Colorado. Philadelphia (PA): AACR; Cancer Res 2023;83(11 Suppl):Abstract nr A061.

P40 | Influence of oral exposure to low concentration of fumonisin B2 on the immune system

Journal of Veterinary Pharmacology and TherapeuticsVolume 46, Issue S1 p. 104-105 POSTER PRESENTATIONS P40 | Influence of oral exposure to low concentration of fumonisin B2 on the immune system First published: 11 June 2023 https://doi.org/10.1111/jvp.13319Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article. Volume46, IssueS1Special Issue: 15th International Congress of the European Association for Veterinary Pharmacology and Toxicology held Bruges, Belgium, July 2–5, 2023June 2023Pages 104-105 RelatedInformation