Structural conformation of triple-helical poly(dT)-poly(dA)-poly(dT) has been a very controversial issue recently. Earlier investigations, based on fiber diffraction data and molecular modeling, indicated an A-form conformation with C ′ 3 − e n d o sugar pucker. On the other hand, Raman, solution infrared spectral, and NMR studies show a B-form structure with C ′ 2 − e n d o sugars. In accordance with these experimental results, a theoretical model with B-form, C ′ 2 − e n d o sugars was proposed in 1993. In the present work we investigate the dynamics and stability of the two conformations within the effective local field approach applied to the normal mode calculations for the system. The presence of counterions was explicitly taken into account. Stable equilibrium positions for the counterions were calculated by analyzing the normal mode dynamics and free energy of the system. The breathing modes of the triple helix are shifted to higher frequencies over those of the double helix by 4–16 cm −1. The characteristic marker band for the B conformation at 835 cm −1 is split up into two marker bands at 830 and 835 cm −1. A detailed comparison of the normal modes and the free energies indicates that the B-form structure, with C ′ 2 − e n d o sugar pucker, is more stable than the A-form structure. The normal modes and the corresponding dipole moments are found to be in close agreement with recent spectroscopic findings.