Azadirachta Research Articles

ANTIMICROBIAL SCREENING, VITAMIN ASSAY AND GCMS ANALYSIS OF CHLOROFORM EXTRACT OF Azadirachta Indica (NEEM) LEAVE.

Azadirachta Indica (Neem) plays a pivotal role in various problems associated with human health. Azadirachta indica is a member of maliaceae family and every part of the Neem tree has some medicinal potential which makes it of great demand in our world today. The plant parts such as leaves, flowers, seeds, fruits, roots and bark have been used traditionally for the treatment of inflammation, fever, infection, dental disorder and skin diseases. The present study aims at identifying and characterizing the different bioactive compound from chloroform neem extract by Gas Chromatography and Mass Spectroscopy (GC-MS) in addition to the study of the antimicrobial potency as well as the vitamin content of neem leaves. Antimicrobial result revealed that chloroform extract of Azadirachta indica has a strong antimicrobial activity against all tested bacteria strain (Salmonella spp, Proteus mirabilis, Staphylococcus aureus and Streptococcus pyogene). Result of vitamin content showed that the vitamin contents are in this order C > B1 > B2 > E. GC-MS analysis revealed presence of 32 compounds. Among the 32 compounds, the most predominate compounds are fatty acids, esters, oleic acid, hydrocarbons, alcohols, aromatics, alkamides, cyanides, steroids, halo compounds, pyrazine, isothiocyanate, thiocyanate, urea and unsaturated alkenamides, alkyne, indol and N-hydroxyimine.

ARBORIZAÇÃO URBANA DE UMA AVENIDA NA ZONA NORTE DE SANTARÉM, PA

A análise da arborização urbana é um elemento fundamental para o planejamento apropriado da arborização de vias públicas e, no município de Santarém, estado do Pará, estudos com esse intuito ainda são escassos. Assim, o objetivo do presente estudo foi avaliar e caracterizar a arborização urbana da Avenida Mendonça Furtado, a fim de subsidiar futuros programas de monitoramento e manejo das plantas nas vias públicas. Para isso, foi realizado um inventário quali-quantitativo completo, no qual foram coletadas as seguintes variáveis: espécie, diâmetro à altura do peito a 1,3 m do solo (cm), diâmetro de copa (m), altura total (m), posição da copa, sanidade da copa, posição do indivíduo, sanidade do fuste, posição da raiz, fiação e manejo. Foram inventariados 220 indivíduos, pertencentes a 15 espécies e a oito famílias. As espécies mais comuns foram: Handroanthus serratifolius; Mangifera indica; Andira inermis e Azadirachta indica. Apurou-se que a arborização é composta por 46,6% de espécies nativas e 53,3% de espécies exóticas. Cerca de 30% das árvores apresentaram conflitos com a fiação, indicando a necessidade de ações de manejo. Os sistemas radiculares da maioria dos indivíduos não causaram danos às estruturas urbanas e não sofreram problemas fitossanitários. Para uma arborização urbana ideal, deve ser criado um plano de manejo, obedecendo a critérios para se ter uma arborização adequada e atendendo as exigências da população.

Eco-friendly management of rice weevil, [(Sitophilus oryzae L.) Coleoptera; Curculionidae] in Rupandehi, Nepal

An experiment to manage rice weevil (Sitophilus oryzae L. Coleoptera, Curculionidae) in wheat (Triticum aestivum L. Gramineae) was carried out at Institute of Agriculture and Animal Science (IAAS), Paklihawa Campus, Nepal. The experiment was conducted under completely randomized design with seven treatments viz. neem (Azadirachta indica A. Juss) @ 15 g/kg, tobacco (Nicotiana tabacum L)@ 10g/kg, ginger (Zingiber officinale Roscoe) @20g/kg, garlic (Allium sativum L.)@20g/kg, sichuwan pepper(Xanthoxylum alatum Roxb.)@10g/kg, sweet flag (Acorus calamus L.) @5g/kg, and control and replicated three times. The highest weevil mortality of weevils was observed in the wheat treated with A. calamus (98.33%,), followed by N. tabacum (85.67%), A. sativum (73.34%), A. indica (70.67%), Z. alatum (70.34%)and Z. officinale (58.34%). Similarly, the lowest percent weight loss (3.32 %) and seed damage (4.0%) was observed in wheat treated with A. calamus. Moreover, germination (89%) was observed in seeds treated with A. calamus when tested after 90 days of treatment application. Based on weevil mortality and germination test in this experiment, it is found that sweet flag is the best treatment against rice weevil followed by tobacco and garlic respectively. Therefore, these botanicals could be one of the effective alternatives for the management of weevil especially to the farmers who do not use chemical insecticides in rural area of Nepal.

Insecticidal Activities of Some Botanicals on the Three Species of Callosobruchus

Nine medicinal and aromatic plants were evaluated for their efficacy as contact insecticides on the three species of Callosobruchus, i. e. C. maculatus, C. analis and C. chinensis. Aqueous extract of twelve plants, i. e. Synzgium aromaticum (clove floral buds), Azadirachta indica (neem leaves), Allium sativum (garlic bulbs), Piper nigrum (black pepper seeds), Thevetia peruviana (kaner leaves), Moringa oliefera (moringa leaves, flowers, drumstick and root), Prosopis cineraria (khejri leaves), Eucalyptus globulus (eucalyptus leaves) and Curcuma longa (turmeric rhizome) were tested against all the three species of bruchids. The extracts were applied at doses of 0.5, 1.5, 2.5, 3.5 and 4.5 ml/20 g to cowpea seeds by filter paper diffusion method. The higher dose of flower and leaf extracts from M. oleifera, seed extract from P. nigrum and leaves extract from A. indica have shown potent insecticidal activity against all the three Callosobruchus species. Use of these botanicals can be an ecofriendly alternative to the chemical insecticides against the Callosobruchus species.

Dermal Exposure of Morinda Citrifolia Linn. Fruit Extract Coated Gold Nanoparticles: Effects on Haematological and Biochemical Parameters in Wistar Rats

The present study was carried out to evaluate the effect of Morinda citrifolia fruit extract coated gold nanoparticles on subacute dermal toxicity in wistar rats. Gold nanoparticles (GNPs) were synthesized by biological reduction (green synthesis) of chloroauric acid (HAuCl 4 .3H 2 O) with Azadirachta Indica (Neem) 5% leaf extract1. The nanoparticles characterized and were confirmed by colour changes from pale yellow to ruby red. These nanoparticles were coated with Morinda citrifolia fruit extract (McFE)1. The toxicity was evaluated in 50 wistar rats of either sex and randomly assigned to five groups. One group was considered healthy, three groups received dermal application of the combination of 1 mg/kg GNPs with 150 mg/kg McFE (Low dose), 2 mg/kg GNPs with 300 mg/kg McFE (Medium dose) and 4 mg/kg GNPs with 600 mg/kg McFE (High dose) dermal application for 28 days. The last group was considered as satellite group which received 8 mg/ kg GNPs with 1200 mg/kg McFE and animals were observed for extra 14 days beyond trial period for post withdrawal effect. There were no abnormal behavioral signs and no any alteration observed on skin throughout the experimental period. Hematological parameters showed no significant variations as a sign of toxicity except hike in neutrophil count and compensatory increase in TEC count on 28th day of entire treatment period but not in a dose dependent manner. Similarly, serum biochemical values showed a hike in ALT and AST in McFE coated GNPs groups. However, hike in BUN values in the experiment was observed to be non-dose dependent and a significant increase in creatinine dose independently on 28th days was observed. There was non-significant increase in total protein on day 14 was observed in the experiment. There was no significant toxicity in entire dosage, from hematology aspect but slight immune stimulation seen up-to 14th day of experiment (neutrophil) in McFE coated GNPs doses with no relation to dose and coating. However, in serum biochemical value slight liver functions tests affected on 14th day but no specific dose related toxicity is observed in any of the treatment. There is increase BUN and creatinine level on 28th day but proper excretion by kidney filtration units. A chronic toxicity trial is appealed to understand the extent of dermal toxicity.

In-vitro Studies of Anti-EGFR Tyrosine Kinase Activity of Thai nutraceutical Plants.

Functional foods have emerged as a new approach to improve human health in term of nutraceutical to prevent people from illness rather than cure patients through medical treatment. In Asian society, particularly in Thailand, the utilizations of functional ingredients have been integrated in every parts of ordinary life. In this study, the tyrosine kinase activity of epidermal growth factor receptor (EGFR) inhibiting properties of 23 Thai’s herbs-ethanol extracts have been examined. The crude extracts of only four species that inhibit the activity of EGFR-tyrosine kinase, Azadirachta indica (neem, Sa-dao), Brucea javanica (L.) Merr. (Rajadad), Hibiscus sabdariffa L. (Roselle, Krachiap daeng), and Saccharum chinensis Roxb. (Red sugar cane). Moreover, only ethanol extractions from A. indica and B. javanica were also showed antitumor effect to non-small cell lung cancer, A549 cells.

Immunostimulatory and antifertility effects of neem (Azadirachta indica) leaf extract on common carp (Cyprinus carpio Linnaeu)

180-day feeding trial was conducted on the growth performance, feeding efficacy, immunological parameters and gonadal reproductive parameters of common carp, Cyprinus carpio fed with neem (Azadirachta indica) leaf extract (NLE) incorporated at different levels (0, 0.25, 0.5, 1.0, 1.5 and 2.0 g kg-1 feed) in rice bran and mustard meal (1:1 ratio) basal diet. The study revealed highest Net Weight Gain (NWG) in fish fed on NE 1.0 g kg. Results showed that the good growth response was obtained in fish fed with the NLE 1.0 g kg-1 feed and least in fish fed only on basal diet (without NLE). Whereas, higher gonado-somatic index (GSI), relative fecundity and ova diameter values were obtained from fish fed on basal diet while the least were observed from fish fed only on basal diet, while the least value was fish fed with NLE 1.0 g kg-1 feed. Fish fed with NLE 1.0 g kg-1 feed also showed significantly (p<0.05) enhanced phagocytic activity ratio, Phagocytic index, NBT activity, CAT, SOD, GPx and GR responses in comparison with fish fed only on basal diet, where as LPO is significantly decreased on being fed with NLE up to 1g kg-1 feed. The study indicated that incorporation of NLE in feed improved growth, delayed female gonadal maturation and could be used as a potential immunostimulant and to control the prolificbreeding of C. carpio in aquaculture.

Antimicrobial Activities of Tetracyclic Triterpenoids from the Seeds of A. indica

Two known tetracyclic triterpenoids were isolated from the seeds of Azadirachta indica to evaluate for antimicrobial activities. In this regard, the powdered seeds of Azadirachta indica were extracted using chloroform/methanol (1:1) to provide 60 g of oily crude extract after the removal of the solvent using a rotary evaporator at 50°C. The crude extract was subjected to column chromatographic separation to afford two known compounds identified as azadiradione ( 1 ) and epoxyazadiradione ( 2 ). The crude extract and isolated compounds were evaluated against four bacterial and two fungal strains. Accordingly, the crude extract was found to be active against P. aeruginosa (11 mm) and E. coli (15 mm). Whereas, compound 1 showed significant activity against S.aureus (18 mm) and P. aeruginosa (15 mm).

Arsenic Removal by Using Low Cost Non-conventional (NLP, PLP, ALP) Adsorbents

Abstract In present study, Azadirachta indica (neem leaves), Ficus religiosa (peepal leaves), Emblica officinalis (amla leaves) used as adsorbent. Batch experiments were conducted to study the adsorption behaviour. The material was characterized by FTIR, XRD, TGA-DTA, and SEM-EDS. The extent of arsenic removal capacity was tested by the material by varying the solution parameters like adsorbent dose, adsorbate concentration and pH of the solution, contact time and temperature. The amount of arsenic adsorption increases with the increase of adsorbent concentration which indicates that adsorption depends upon the availability of active sites for arsenic. The experimental data were correlated reasonably well by Freundlich adsorption isotherm. The optimum conditions for the removal of arsenic from water are initial concentration: 0.1 mg/L, adsorbent dose: 1 g/50 mL, pH: 6.0, temperature 50oC±2oC, contact time: 30 min. and shaking time: 1 h is worked out. Increasing order of arsenic adsorption on leaf powders was as follows: NLP > ALP > PLP. It can therefore, be potentially applied for the treatment of water contaminated by arsenate. Keywords: Adsorption; low cost adsorbent; Arsenic concentration; Water treatment Cite this Article Shikha Saxena, Nisha Singh, Nidhi Jain, Arsenic Removal by Using Low Cost Nonconventional (NLP, PLP, ALP) Adsorbents Journal of Modern Chemistry & Chemical Technology . 2018; 9(3): 29–39p.

Bio-Synthesis of Silver Nanoparticle Using Azadirachta indica Leaves

In this study, simple approach was attempted to obtain a green eco-friendly way for the bio-synthesis of metal nanoparticles (AgNPs) from bioreduction of AgNO3 solutions using Azadirachta indica leaf extracts. The formation of nanoparticle and its stability were examined through UV-Visible spectra, FTIR spectra, and Zeta potential measurements. The exposure of metal ions to these plant extract by UV-Vis confirmed the presence of silver nanoparticles. FTIR revealed the reduction of silver ions, the functional groups present in the plant extract. The stability of the AgNPs was confirmed with the zeta potential measurement.

Natural products in regulation of male fertility.

Medicinal plants may prove useful in developing plant-based strategies for regulation of male fertility. The present review describes the antifertility potential of certain medicinal plants, viz. Azadirachta indica, Curcuma longa, Allamanda cathartica and Bacopa monnieri in Parkes (P) male mice. The results suggested that treatment with the aqueous extracts of these plants caused reversible suppression of spermatogenesis and fertility in P mice and that there were no signs of detectable toxicity in treated mice. Further research needs to be done to develop plant-based strategies for control of male fertility.

HPTLC fingerprinting and antimicrobial activity of Azadirachta indica leaf extracts

Azadirachta indica Juss which also Known as Neem plant used from ancient times for various human ailments as a traditional medicine. To appraise the scientific study for the use of Azadirachta indica, ethanolic extracts of the dried leaves of the plant were subjected to HPTLC finger printing and determination of anti-microbial activity of aqueous and ethanolic extract on five different species of bacteria and a fungus. The ethanolic extract of dried powdered leaves was evaluated by HPTLC finger printing using solvent system of Dichloromethane: ethanol (19:1) which exhibits the identity of different compounds present in the extracts. The antimicrobial activity of the concentrated extracts was evaluated by determination of the diameter of zone of inhibition against the microorganisms using agar well diffusion method. Both plant extracts had antimicrobial effects against the test organisms, however the aqueous extracts were found to show greater anti-microbial effect than ethanolic extract. Thus, the mean diameter zones of inhibition ranged from 0.04mm-50.00mm for aqueous extract and 0.40mm-20.00mm for ethanolic extract at the highest concentration of 50mg/ml. The finding of this study supports the use Azadirachta indica leaves and can be use in the treatment of various microbial infections as alternative systems of medicine.

Some Classic Formulation used by the Native Practitioners of South Tripura District, Tripura, North-East India

This article highlights some classic formulations used by the traditional practitioners of Bangalee community in Belonia bio – region of South Tripura district for the treatment of various ailments. A field survey revealed nine classic formulations those are important folklore medicines for various ailments, where 23 different plants species and five organic and inorganic entities have identified those used as components for nine formulations and two species of plants Citrus limon Linn, Azadirachta indica (A). Juss recorded in more than one formulation. The name and address of traditional practitioners, the composition of formulations, disease, sign and syndrome have detailed in this article. Apart from traditional knowledge few traditional mindset on ghost action, restriction for pregnant women has also documented. With the advent of the modern exotic culture and the parallel development of modern or allopathic medicines, the rich Indian traditional system of health care practices have sidelined and neglected. Resultantly, the village-based wisdom of the traditional system of health care practices has not seriously referred, not well documented and conveyed properly over generations. With this background, our survey on Tripura traditional medicine (TTM) may help in the discovery of safe, natural remedy for some dreaded ailments and sustainable development of ethno-medicinal bioresources.

Antifungal potential and defense gene induction in maize against Rhizoctonia root rot by seed extract of Ammi visnaga (L.) Lam.

Methanol extracts from five medicinal plants ( Eucalyptus tereticornis Sm., Ammi visnaga (L.) Lam., Azadirachta indica A. Juss., Rheum Palmatum L., and Adansonia digitata L.) were assessed in vitro for antifungal activity against Rhizoctonia solani Kuhn, the causal agent of Rhizoctonia root rot of maize. All tested extracts showed antifungal activity with varied extents. Ammi visnaga (khella) extract showed the greatest activity compared with the untreated experimental controls. Observations using transmission electron microscopy showed ultrastructural changes in hyphal cells as a response to exposure to khella extract. Gas chromatography-mass spectrometric analysis of khella extract showed the presence of 69 compounds. The antifungal properties of the extracts were mainly attributed to their content of coumarins and fatty acids. In the greenhouse experiment, treatment of maize plants with khella extract at 15% gave the least incidence of Rhizoctonia root rot. Results of DD-PCR showed up- and down- regulations of some genes in maize as response to the treatment with khela extract. Identification of the randomly selected genes from DD-PCR revealed that they were defense-related, as S-domain class receptor-like kinase 3 and glutathione-S-transferase1. Real-time PCR showed induction in the gene expression of the pathogenesis-related protein chitinases (2.36 fold) and thaumatin-like proteins (8.99 fold) by treatment with khella extract at 15%, which was greater expression than detected at 10 and 20%. This indicates triggering effects from the extract on the maize immune system against the R. solani infection in a concentration-dependent manner. The efficient, low-cost and eco-friendly characteristics of khella extract indicate that it could be used for the control of Rhizoctonia root rot of maize.

P-gp modulating effect of Azadirachta indica extract in multidrug-resistant cancer cell lines.

The extract of Azadirachta indica, commonly known as neem, has found extensive use in traditional medicine for treating various human diseases. In this study, the effect of the 50% ethanol extract of A. indica (AI01) on P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) was examined using MDR cell lines, specifically paclitaxel-resistant HepG2 (PR-HepG2) and doxorubicin (DOX)-resistant (DR) colon-26 cells. 96-h treatment of the two cell lines with AI01 (30 μg/mL) showed no effect on the expression of P-gp mRNA (human MDR1 and mouse mdr1b) and protein, while AI01 increased the accumulation of rhodamine 123, a P-gp substrate, in both PR-HepG2 and DR-colon-26 cells. The cytotoxic effects of 48-h treatment with AI01 on the viability of PR-HepG2 and DR-colon-26 cells were not observed. Therefore, 30 μg/mL AI01 may have no cytotoxic and P-gp-inducing effects. Finally, AI01 potentiated the sensitivity of PR-HepG2 and DR-colon-26 cell lines to DOX by 8.6- and 15.3-fold, respectively. These findings suggest that A. indica may be a promising source for a new class of P-gp modulators without cytotoxic/P-gp induction effects.

Chemical composition of neem and lavender essential oils and their antifungal activity against pathogenic fungi causing ginseng root rot

The aim of this study was to assess in vitro, the antifungal activity and characterize the chemical constituents of the essential oils of Azadirachta indica (neem) and Lavandula angustifolia (lavender). The essential oils of A. indica and L. angustifolia plants were tested against several isolates of pathogenic fungal genera causing root rot disease of ginseng. Agar plate assay indicated that lavender oil at 10% exhibited the highest inhibition index of (86.0±0.7%) against Sclerotinia nivalis mycelial growth. Neem and lavender oils at 5% v/v showed inhibition index against Alternaria panax (72.9±2.1 and 45.0±1%, respectively). Lavender oil at 5% (v/v) inhibited growth of S. nivalis (83.1±0.2%) and Cylindrocarpon destructans (49.2±1%). The gas chromatography-mass spectrometry (GC-MS) analysis showed that the major constituents of neem oil were fatty acids (94.8%). However, sesquiterpenes were the dominant constituents of the lavender oil (57.6%). The antifungal indices demonstrated in this study are a clear evidence of the potentiality of neem and lavender essential oils to control plant diseases caused by phytopathogenic fungi. Key words: Ginseng root rot, fungi, essential oils.

Studies on antibacterial spectrum of extracts of stem bark of Azadirachta indica A. Juss on some clinical isolates associated with urinary tract infection

This investigation was aimed at determining the antibacterial activity of extracts of stem bark of Azadirachta indica A. Juss on some clinical isolates. Results of preliminary analysis showed that the stem bark of A. indica possesses alkaloids, flavonoids, tannins, cardiac glycosides, phlobotannins and phenols. The ethanol extract produced higher antibacterial activity against all test microorganisms compare to the acetone extract, the highest Proteus vulgaris (15 mm) with ethanol extract at 500 mg/ml, and Staphylococcus aureus (13.5 mm) with acetone extract at 500 mg/ml respectively. Both the extracts produced definite strong antibacterial activity against test organisms at 50 to 1000 mg/ml concentration. Statistical analysis (t cal.-0.42) revealed that there was no significant difference among the antibacterial activities of ethanol and acetone extracts of A. indica against the test organisms. At pH value 2.0, the antibacterial activity of ethanol extract at 500 mg/ml was increased significantly against P. mirabilis (diameter of zone 20.0 mm) compared to the untreated one at pH 4.2 (diameter of zone 9.0 mm). In the case of S. aureus at pH 8.0 there was an increase in antibacterial activity of the ethanol extract (diameter of zone 29.0 mm) when compared to untreated extract at pH 4.2 (diameter of zone 14.0 mm). The minimum inhibitory concentration (MIC) values for acetone extract ranged from 100 to 500 mg/ml for the test organisms, and for ethanol extract the value was 200 mg/ml for all the test organisms. In case of test organisms, the minimum bactericidal concentration (MBC) value was 500 mg/ml for both extracts. So, the use of A. indica for treatment of urinary tract infection may be justified. Key words: Phytochemicals, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), Azadirachta indica, Proteus mirabilis.

Preliminary findings on provenance trial of Azadirachta indica in Western Terai, Nepal

No Abstract

Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: Evidence from anti-inflammatory, antioxidant and anti-apoptotic studies.

Chronic neuropathic pain is a common and widely recognized pain syndrome for patients and difficult to manage for physicians. Azadirachta indica (AI) possesses analgesic, anti-inflammatory, and antioxidant properties. To evaluate the neuroprotective effect of AI standardized extract in an animal model of peripheral neuropathy induced by partial sciatic nerve ligation (PSNL). PSNL was induced in male Wistar rats (180-200 g) with tight ligation of the nerve. Rats received treatment with either vehicle i.e. distilled water (PSNL control), Pyridoxine (100 mg/kg, p.o.) or AI (100, 200 and 400 mg/kg, p.o.) for 28 days. Various behavioral parameters, biochemical, molecular and histological parameters were evaluated. PSNL resulted in a significant decrease (p < 0.05) in allodynia, hyperalgesia, motor coordination and motor nerve conduction velocity (MNCV) whereas chronic treatment with AI (200 and 400 mg/kg) significantly attenuated (p < 0.05) these behavioral changes. Enhanced activity of oxidative-nitrosative stress, inflammatory mediators (TNF-α, IL-1β, and NF-κB) as well as mRNA expression of Bax, Caspase-3, and iNOs were significantly attenuated (p < 0.05) by AI treatment. It also significantly increased (p < 0.05) peripheral blood oxygen content and Bcl-2 mRNA expression. The flow cytometric analysis revealed that AI (200 and 400 mg/kg) treatment significantly attenuated neural apoptosis and reactive oxygen species levels. PSNL induced histological aberrations were also decreased by AI treatment. Azadirachta indica exerts its neuroprotection against PSNL induced neuropathic pain via inhibition of oxidative-nitrosative stress, the release of pro-inflammatory cytokines and apoptosis to improve MNCV (graphical abstract, Figure 1(Fig. 1)).

The Potency of Neem (Azadirachta indica A. Juss) Product and Factors affecting The Neem Leaf Production in Lombok

ABSTRACT Neem ( Azadirachta indica A. Juss ) is a type of non-timber forest product, the leaves could produce plant-based for bio-pesticides and antiseptics. This study aimed to obtain information on potential stands, leaf production and its oil content and the factors that affecting the neem leaf potency. Research was done purposively by the surveying method and tree measurements was census in each location. The neem leaf potency per tree in East Lombok is 7.4 to 21.6 kg, oil content and rendement is 0.07 to 0.20 kg and 1.88 to 2.33%. North Lombok are, 8.5 to 21.2 kg for potential leaf, and 0.14 to 0.18 kg for oil content and 2.17 to 4.34% for ist oil rendement. Neem leaf production is influenced by branches and sub number, diameter and crown width, while a site less influential (elevation and soil pH). The parameter use of sub-branches number to get the best model to estimate the potential of leaves, good for one or more variables. The use of all the identified variables (diameter, pertajukan, and a place to grow), obtained 48.9% R-square. Means more than 50% of other variables that have not been identified that affect the potential of neem leaves. Key words : Neem (Azadirachta indica A. Juss), leaf, East Lombok, North Lombok ABSTRAK Mimba ( Azadirachta indica A. Juss ) merupakan jenis hasil hutan bukan kayu (lHHBK) yang produk daunnya sebagai penghasil bahan pestisida nabati dan antiseptik. Keberadaan mimba cukup potensial di Lombok yang tumbuh alami di lahan-lahan kering. Penelitian ini bertujuan untuk memperoleh informasi potensi biomassa daun dan kandungan minyaknya serta faktor-faktor yang mempengaruhi potensi daun mimba. Penelitian dilakukan secara purposive dengan metode survai dan pengukuran dimensi pohon secara sensus pada setiap lokasi. Potensi daun mimba per pohon di Lombok Timur adalah 7,4 – 21,6 kg, kandungan minyak dan rendemennya 0,07 – 0,20 kg dan 1,88 – 2,33 %. Di Lombok Utara potensi daun, kandungan minyak dan rendemennya adalah 8,5 – 21,2 kg per pohon, 0,14 – 0,18 kg per pohon dan 2,17 – 4,34 % . Stok biomassa daun mimba per kecamatan adalah Jerowaru 0,31 ton, Keruak 0,31 ton, Sakra 0,32 ton, Pringgabaya 0,13 ton, Sambelia 0,13 ton dan Kecamatan Bayan 0,69 ton. Produksi daun mimba sangat dipengaruhi oleh jumlah ranting, jumlah cabang, diameter dan lebar tajuk pohon, sedangkan faktor tempat tumbuh kurang berpengaruh (elevasi dan pH tanah). Penggunaan parameter jumlah ranting mendapatkan model terbaik untuk menduga potensi daun, baik untuk satu variabel maupun lebih. Penggunaan semua variabel yang teridentifikasi tersebut (diameter, pertajukan, dan tempat tumbuh), diperoleh R-square 48,9 %. Berarti lebih dari 50 % variabel lain yang belum teridentifikasi yang berpengaruh terhadap potensi daun mimba.