Abstract
Azadirachta Indica (Neem) plays a pivotal role in various problems associated with human health. Azadirachta indica is a member of maliaceae family and every part of the Neem tree has some medicinal potential which makes it of great demand in our world today. The plant parts such as leaves, flowers, seeds, fruits, roots and bark have been used traditionally for the treatment of inflammation, fever, infection, dental disorder and skin diseases. The present study aims at identifying and characterizing the different bioactive compound from chloroform neem extract by Gas Chromatography and Mass Spectroscopy (GC-MS) in addition to the study of the antimicrobial potency as well as the vitamin content of neem leaves. Antimicrobial result revealed that chloroform extract of Azadirachta indica has a strong antimicrobial activity against all tested bacteria strain (Salmonella spp, Proteus mirabilis, Staphylococcus aureus and Streptococcus pyogene). Result of vitamin content showed that the vitamin contents are in this order C > B1 > B2 > E. GC-MS analysis revealed presence of 32 compounds. Among the 32 compounds, the most predominate compounds are fatty acids, esters, oleic acid, hydrocarbons, alcohols, aromatics, alkamides, cyanides, steroids, halo compounds, pyrazine, isothiocyanate, thiocyanate, urea and unsaturated alkenamides, alkyne, indol and N-hydroxyimine.
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