As the centrifugal pump is running, the fluid usually flows into the impeller along pump shaft, and the fluid flows out radially by the force of the impeller. The force is mutual, so the impeller is also subjected to the reaction force of the fluid, but the distribution of this force on the blades is uneven. In addition, the front and rear shrouds of the impeller are asymmetric, which are the main causes of axial force. This paper adopts numerical calculation method studying the mechanism of axial force of impeller at all stages of multistage pump at various working conditions, and exploring the formation mechanism of shroud pressure differential force and blade twisting axial force and its variation laws of similarities and differences, analyzing the steady state and transient characteristics between axial force and hydraulic property of double-casing multistage pump. The results show that the rotational angular velocity of the fluid in the front and rear pump chamber at each stage impeller is distributed along the axial direction in three regions, the regions are pump body boundary layer, core region, and impeller boundary layer. The working surface and back surface of the blade twist have the high and low axial force area, and its distribution is staggered, at the same number of stages, the greater the flow rate, the smaller the blade twisting axial force. The shroud pressure differential force with the increase of impeller stages presents a linear increasing trend, conforms to the principle of linear superposition of cover pressure differential force. The total axial force pulsation of multi-stage pump is related to the number of secondary impeller blades, its primary frequency coincides with the secondary impeller blade frequency, increasing the flow rate can reduce the multi-stage pump axial force pulsation amplitude. The pulsation period of single-stage impeller head and efficiency are related to the number of impeller blades, the smaller the number of impeller stages, the stronger the pressure dynamic, and static interference effect of the impeller inlet and outlet. Rotation of the secondary impeller causes dynamic and static interference, which is the main reason for the pulsation of the axial force coefficient in double-casing multistage pumps, the pulsation intensity is related to the periodic generation and shedding of the blade vortex. The results of the study can be used as a reference for optimizing the axial force of double-casing multistage pumps.